LEADING THE OMEGA-3 FUTURE - POWERED BY TRANSBIODIESEL - EnzymoCore

LEADING THE OMEGA-3 FUTURE

 POWERED BY TRANSBIODIESEL

Leading the Omega-3 future

 Cutting-edge proprietary enzymatic technology for the production of concentrated Omega- 3 (>60%) with:
   Advanced nutritional potential

   Unique, high-potency EPA + DHA combinations, designed for specific functions such as brain and heart support

   Predetermined ratio of DHA and EPA in the form of mono-, di- and tri-glycerides, ethyl esters, phospholipids and glycolipids

   Higher bioavailability

 Our revolutionary biocatalysts OmegaZyme ET™ and TransZyme ET ™ :
   Enable smart engineering and restructuring of lipids

   Increase processes efficiency

   Significantly lower production costs

                                                                                                               Powered by TransBiodiesel

General: Health benefits offered by the
                                     consumption of EPA/DHA

 Reducing LDL-cholesterol, blood pressure and coronary heart diseases such as heart attacks and strokes

 Improving body functions including blood clotting, fertility, muscle activity, digestion and cell division & growth

 Infants: DHA promotes brain, nerve and eye development.

                                                      http://www.grandviewresearch.com/industry-analysis/omega-3-market

The Omega-3 PUFA market is segmented

Type: Docosahexaenoic acid (DHA), Eicosapentaenoic acid (EPA) and Alpha Linolenic acid (ALA)
Application:
  Dietary supplements
  Functional food & beverages
  Pharmaceuticals
  Infant formula
  Others.
Source: Marine and plant
  Marine source is further segmented into fish oil, algal oil, krill oil, and others.
  Plant source is further segmented into flax and chia seeds oils, and others.

                                              http://www.nutritionaloutlook.com/omega-3/2016-ingredient-trends-watch-food-drinks-and-dietary-supplements-omega-3
                                              https://www.fractovia.org/news/industry-research-report/omega-3-ingredients-market

High concentrates source is estimated to be a major revenue contributor
                        in omega-3 ingredients market
   A new sub-category (end of 2015): Ultra-high concentrates with EPA and DHA concentrations of up to 90%.
    These ultra-high concentrates are nearing pharmaceutical levels—without being called drugs.
   In October 2015, DSM launched a new line of high concentrate of DHA and EPA products sourced from fish oil
    and a high concentrate of DHA from algal oil. The products contain 85% omega-3 to support cardiovascular
    and cognitive health.
   In June 2015, Croda introduced Incromega DHA, which is ideal for cognitive health and has certification of
    EFSA (European Food Safety Authority).
   Last November, Nordic Naturals launched Omega ONE, an ultra-high concentrate with 80% EPA+DHA (with
    90% or more of that in the absorbable triglyceride form).

Conventional technologies for omega-3 concentrates

 Winterization of fish/algal oils to reach up to 30% in average of omega-3 PUFA

 Transesterification of fish/algal oils to produce fatty acid ethyl esters (FAEEs) which are subsequently:
  1. Subjected to fractional distillation to produce FAEEs with different ratios of EPA/DHA up to 70% in sum

  2. Supercritical carbon dioxide fractionation/column chromatography to reach 50-90% omega-3 concentrates

  3. Urea complexation to reach 60-90% omega-3 concentrates

 Reverse transesterification catalyzed by strong alkaline bases (Sodium/Potassium hydroxide/methoxide) to

   produce r-TAGs with EPA/DHA in the range of 50-90%

Omega-3 PUFA challenges
 Pill size is too big and difficult to swallow
 Fishy burp and taste can make for an unpleasant consumer experience
 Products aren’t potent enough
 Too few specialized products are available to meet specific health needs and perceived value
 Bio-availability
Stability
 Methods of production
 Exposure to high temperatures, low recovery yields and generating large amounts of wastes in production processes

Market size and growth
With the increasing health-consciousness among the consumers and multi-benefits of Omega-3 PUFA, the
demand for Omega-3 PUFA is expected to enhance the market growth from 2015 to 2020.
Global omega-3 market size was USD 1.82 billion in 2014 and is expected to witness substantial growth over the
forecast period owing to increasing demand for functional foods in various countries including India, China, U.S.,
Japan, Australia, Germany, and Italy.
According to Global Market Insights, Inc., “Omega-3 ingredients market size is estimated to grow at a CAGR of
11% over the period of 2015-2022.” The omega-3 ingredients market share is anticipated to surpass USD 3 billion
by 2022.

         North America Omega-3 market revenue, by                     Europe EPA/DHA ingredients (Omega- ,ezis tekram )3
            application, 2012 - 2022 (USD Million)                         2022 &2014 ,noitacilppa yb (USD Million)

Our proprietary Enzymes
TransBiodiesel has developed two new immobilized enzymes for the enrichment of omega-3 fatty acids in
fish/algal oils for the food supplements/nutraceutical industries:

1.    TransZyme ET with different selectivity towards saturated/unsaturated fatty acids/fatty acyl groups for the preparation
      of fatty acid alkyl esters of fish/algal oils

                                      TransZyme ET
      Fish/Algal Oil                                                     FAEEs        +   Glycerol
                                      Ethanol

FAEEs – Fatty acid ethyl esters enriched with up to 70% EPA and DHA.

2. OmegaZyme ET with different selectivity towards saturated/unsaturated fatty acids/fatty acyl groups for the preparation
of enriched omega-3 fatty acids in the form of glycerides

                                     OmegaZyme ET
      Fish/Algal Oil                                                     Glycerides       +   FAEEs
                                     Ethanol
Glycerides enriched with 50-70% EPA and DHA in the form of glycerides.

TransBiodiesel’s proprietary enzymatic technology
TransBiodiesel's technology platform, consisting of two elements:
  Modification and adaptation of enzymes (lipases) for use in solvent-free systems
  Immobilization of modified enzymes for industrial applications, which is achieved by binding the enzymes to specific carriers

TransBiodiesel's technology overcomes the major drawbacks of enzyme-based catalysts of Omega-3 products:
  High cost of the enzymes
  Low efficiencies
  Low long-term stability

  The modified immobilized enzymes developed by TransBiodiesel can be used in multiple batch and in continuous processes
  They are highly active in solvent-free systems
  TransBiodiesel's biocatalysts are capable of catalyzing the esterification/transesterification/interesterification reactions simultaneously,
   which allows them to be used for the production of mono-, di- and tri-glycerides, ethyl esters, glycolipids and phospholipids

HighOmega

 Ultra-pure, high-potency EPA and DHA >> 60%
 Tailored solutions with advanced nutritional potential
 HighOmega technology can create unique EPA + DHA combinations, designed for specific health benefits
  such as brain and heart support
 Produced using enzymatic reactions, lipid- engineering independent of raw material variations

 Smaller, easier to swallow pills
 Reduced costs for encapsulation, transport, and display of finished supplements

Competition
Company name                Trademark        High+       Enzymatic   Production   Ethyl esters   Mono- , di--, & tri-   Phospholipids/    Source
                                           Ultra high                   Cost                        glycerides            Glycolipids
                                          concentrates
TransBiodeiesel           HighOmega          50-80%         +           low            +                  +                   +          Fish/ algal

DSM Nutritional           MEG-3®             > 85%           -          high           +                  +                   -          Fish/ algal
Products

BASF Human Nutrition      Lovaza             >85%            -          high           +                  +                   -             Fish

Croda International PLC   Incromega DHA     70% min          -          high           +                  -                   -             Fish
(U.K.)                    OmeRx™.
Polaris, france           Omegavie®          30-80%         +           high           +                  +                   -          Fish/alga

GC-Reiber                 VivoMega           75-90%         +           high           +                  -                   -             Fish
Norway
Arctic Nutrition          Romega®            50-80%          -          high           +                  -                   +             Fish

Organic Technologies      AlaskOmega®        50-80           -          high           +                  -                   -             Fish

COMPANY PROFILE
                                                                                                            FOUNDED IN 2007 BY DR. SOBHI BASHEER
TransBiodiesel produces immobilized enzyme-based catalysts for the production of fatty acid alkyl
esters using high-acid oils feedstocks of potential application for:
                                                                                                            DEVELOPED THE WORLD'S ONLY IMMOBILIZED
1.      The 2nd and 3rd generation biodiesel fuel complying with the specs.                                 ENZYME-BASED CATALYST FOR THE INDUSTRIAL
                                                                                                            SCALE PRODUCTION OF BIODIESEL

2.      The enrichment of omega-3 fatty acids in the form of fatty acid alkyl esters and in the form of
        glycerides.
                                                                                                            7 PATENTS & PROPRIETARY KNOW-HOW

Our biocatalysts are environment-friendly, enable the catalysis of esterification and transesterification   EU ENTERPRENEUR OF THE YEAR 2014
of oil feedstocks,with different selectivity to the various fatty acids, while lowering significantly the
total production costs of production.                                                                       STATE OF ISRAEL - 2010 START-UP OF THE
                                                                                                            YEAR

                                                                                                            2015: ESTABLISHED FIRST JV IN LIMA / PERU
                                                                                                            FOR THE PRODUCTION OF BIODIESEL

                 TransZyme A/ TransZyme ET/ OmegaZyme

CONTACT DETAILS

FOR FURTHER INFORMATION:

EMAIL:        INFO@TRANSBIODIESEL.COM
PHONE:        +972-4-770-9182
FAX:          +972-4-770-9188
CELL:         +972-54-330-1110

WEB:          WWW.TRANSBIODIESEL.COM

ADDRESS:      P.O. BOX 1063 | SHFAR-AM
              20200 | ISRAEL