Product Datasheet p21/CIP1/CDKN1A Antibody (WA-1) - Novus Biologicals
←
→
Page content transcription
If your browser does not render page correctly, please read the page content below
Product Datasheet
p21/CIP1/CDKN1A Antibody (WA-1)
NBP2-29463
Unit Size: 0.1 mg
Store at 4C.
Publications: 13
Protocols, Publications, Related Products, Reviews, Research Tools and Images at:
www.novusbio.com/NBP2-29463
Updated 8/24/2020 v.20.1
Earn rewards for product
reviews and publications.
Submit a publication at www.novusbio.com/publications
Submit a review at www.novusbio.com/reviews/destination/NBP2-29463Page 1 of 4 v.20.1 Updated 8/24/2020
NBP2-29463
p21/CIP1/CDKN1A Antibody (WA-1)
Product Information
Unit Size 0.1 mg
Concentration 0.2 mg/ml
Storage Store at 4C.
Clonality Monoclonal
Clone WA-1
Preservative 0.05% Sodium Azide
Isotype IgG1 Kappa
Purity Protein A or G purified
Buffer 10 mM PBS with 0.05% BSA
Target Molecular Weight 21 kDa
Product Description
Host Mouse
Gene ID 1026
Gene Symbol CDKN1A
Species Human, Mouse, Rat, Primate
Specificity/Sensitivity This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor
suppressor protein. This MAb is highly specific to p21 and shows no cross-
reaction with other closely related mitotic inhibitors. p21WAF1 is a specific
inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a
variety of malignancies. The expression of this gene acts as an inhibitor of the
cell cycle during G1 phase and is tightly controlled by the tumor suppressor
protein p53. Its expression is induced by the wild type, but not mutant, p53
suppressor protein. Normal cells generally display a rather intense nuclear p21
expression. Loss of p21 expression has been reported in many carcinomas
(gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA,
MAb WA-1 is useful either as a solid phase or for detection of p21 protein.
Immunogen Recombinant human p21 protein was used as immunogen to generate the
antibody.
Product Application Details
Applications Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence,
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-
Paraffin
Recommended Dilutions Western Blot, Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry 0.5-
1ug/ml, Immunocytochemistry/Immunofluorescence 1-2ug/ml,
Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-
1ug/ml
Application Notes Human Chromosome Location: 6p21.31 Use in ELISA has been reported in
literature.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate
buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Use in
Western blot reported in scientific literature (PMID 28137862). The 7mL size is a
pre-diluted size and no additional dilutions are required before using this item for
the intended application.Page 2 of 4 v.20.1 Updated 8/24/2020 Images Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
Page 3 of 4 v.20.1 Updated 8/24/2020 Publications Couture R, Mora N, Al Bittar S, et al. Luteolin modulates gene expression related to steroidogenesis, apoptosis, and stress response in rat LC540 tumor Leydig cells Cell Biol. Toxicol. 2019 Jun 14 [PMID: 31201582] Aratani S, Tagawa M, Nagasaka S et al. Radiation-induced premature cellular senescence involved in glomerular diseases in rats. Sci Rep. Nov 14 2018 12:00AM [PMID: 30429495] (IHC-P, Rat) Huang X, Huang R, Gou S et al. Platinum(IV) complexes conjugated with phenstatin analogue as inhibitors of microtubule polymerization and reverser of multidrug resistance Bioorg. Med. Chem. 2017 Jul 08 [PMID: 28728896] (Human) Xiang J, Yang S, Xin N et al. DYRK1A regulates Hap1-Dcaf7/WDR68 binding with implication for delayed growth in Down syndrome Proc. Natl. Acad. Sci. U.S.A Jan 30 2017 12:00AM [PMID: 28137862] (WB, Mouse) Ge X, Pettan-Brewer C, Morton J et al. Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis 2015 Sep 24 [PMID: 26400209] (IHC, Mouse) Uy D, White ET, Oka T. Observation of Delta|k - l | = 3 Transitions in the nu3 Band of H3 O+ J Mol Spectrosc. 1997 Jun [PMID: 9252294] (WB) Details: WB (NIH3T3, MEF, ES EPL cells), Figs 1D, 3A. Rossner P Jr, Binkova B, Milcova A et al. Air pollution by carcinogenic PAHs and plasma levels of p53 and p21(WAF1) proteins. Mutat Res. 2007 Jul 1 [PMID: 17408702] (Human) Details: Direct ELISA (human blood plasma), CLONE SPECIFC Tables 1-3. Note: The specificity of the p21 antibody for p21 was validated with recombinant p21 by direct ELISA in Tables 1-3. Rossner P Jr, Binkova B, Chvatalova I et al. Acrylonitrile exposure: the effect on p53 and p21(WAF1) protein levels in the blood plasma of occupationally exposed workers and in vitro in human diploid lung fibroblasts. Mutat Res. 2002 May 27 [PMID: 12034325] (WB) Details: Direct ELISA (human blood plasma), Tables 3-5; WB (primary human lung fibroblasts), Fig 3. The specificity of the p21 antibody for p21 was validated with recombinant p21 by direct ELISA in Tables 3-5. Nobre RJ, Herraez-Hernandez E, Fei JW et al. E7 oncoprotein of novel human papillomavirus type 108 lacking the E6 gene induces dysplasia in organotypic keratinocyte cultures. J Virol. 2009 Apr [PMID: 19153227] (WB, IHC-P, Human) Details: Human keratinocyte oragnotypic raft cultures: IHC (paraffin), Fig3A; WB, Fig 3B. Blaydes JP, Luciani MG, Pospisilova S et al. Stoichiometric phosphorylation of human p53 at Ser315 stimulates p53- dependent transcription. J Biol Chem. 2001 Feb 16 [PMID: 11078726] (WB) Details: WB (SAOS), Fig 5. Scott MT, Ingram A, Ball KL. PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein. EMBO J. 2002 Dec 16 [PMID: 12485998] (WB) Details: WB: (HeLa), Fig 3D. Romanov VS, Pospelov VA, Pospelova TV. Cyclin-dependent kinase inhibitor p21(Waf1): contemporary view on its role in senescence and oncogenesis. Biochemistry (Mosc). 2012 Jun [PMID: 22817456] More publications at http://www.novusbio.com/NBP2-29463
Novus Biologicals USA Bio-Techne Canada
10730 E. Briarwood Avenue 21 Canmotor Ave
Centennial, CO 80112 Toronto, ON M8Z 4E6
USA Canada
Phone: 303.730.1950 Phone: 905.827.6400
Toll Free: 1.888.506.6887 Toll Free: 855.668.8722
Fax: 303.730.1966 Fax: 905.827.6402
novus@novusbio.com canada.inquires@bio-techne.com
Bio-Techne Ltd General Contact Information
19 Barton Lane www.novusbio.com
Abingdon Science Park Technical Support: technical@novusbio.com
Abingdon, OX14 3NB, United Kingdom Orders: orders@novusbio.com
Phone: (44) (0) 1235 529449 General: novus@novusbio.com
Free Phone: 0800 37 34 15
Fax: (44) (0) 1235 533420
info.EMEA@bio-techne.com
Products Related to NBP2-29463
HAF007 Goat anti-Mouse IgG Secondary Antibody [HRP (Horseradish
Peroxidase)]
NB720-B Rabbit anti-Mouse IgG (H+L) Secondary Antibody [Biotin]
NBP1-43319-0.5mg Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1)
NBP2-22976 Recombinant Human p21/CIP1/CDKN1A Protein
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis.
Primary Antibodies are guaranteed for 1 year from date of receipt.
For more information on our 100% guarantee, please visit www.novusbio.com/guarantee
Earn gift cards/discounts by submitting a review: www.novusbio.com/reviews/submit/NBP2-29463
Earn gift cards/discounts by submitting a publication using this product:
www.novusbio.com/publicationsYou can also read
