ZOONO Z-71 Efficacy Test Results

Efficacy Test Results

ZOONO Z-71

Zoono Z-71 Residual Antimicrobial Technology
                              A Game Changer

Zoono Antimicrobial Residual Technology
Zoono is an antimicrobial technology that works differently from the common liquid traditional
disinfectants that have been used for many years. Antibiotics have lost efficiency in the
treatment of infection, due to resistance developed by potentially harmful microorganisms.
This is the result of a global misuse of antibiotics through poor prescribing, agriculture and
availability without prescription. Some bacteria are now totally resistant to previously
considered most effective antibiotics such as Carbapenemas. The latter (Carbapenemas)
will be inactivated and destroyed by the bacteria producing an enzyme called
Enterobacteriaceae (CPE).
Zoono technology is not an antibiotic but it is a game changing solution to reduce the
transmission of pathogenic microorganisms in the environment. It is the next generation of
antimicrobial surface protection as it continues to kill microorganisms for up to 30 days.
Zoono works when it is dry, it can be applied to any surface with a spray, a wipe or more
sophisticated fogging techniques. Once it has been applied Zoono binds to the surface
producing a protective antimicrobial barrier. Aesthetic cleaning can be carried out if
necessary.
Why is protecting surfaces important?
It is now widely acknowledged that both the environment and surfaces are a significant
source of microbiological contamination and are considered a major vector for harmful
microorganisms to cause infection (cross-infection). Bacteria, viruses, fungi and yeasts
contaminate surfaces and can form bio-films that are extremely resilient to hygiene
processes i.e. cleaning and disinfection. Harmful microorganisms can be transferred by
cross contamination via touch or the air in the form of aerosols (in water droplets) or
generally as single cells or “clumps”. Aerial contamination is especially significant for viral
infections but also for other infections such as those caused by spores such as Clostridium
Difficile. Bacteria such as Pseudomonas spp can also be found in water systems. Each
species and family of microorganisms can have its own unique mode of transmission and
portal of entry. People with a weak immune system, the very young and very old can be
especially vulnerable and ideal targets. However, there are a plethora of microorganisms
“pathogens” that can infect any person, such as Staphylococcus aureus (MRSA), Norovirus,
Aspergillus Niger and extremely pathogenic viruses such as Ebola. The most likely to
succumb to these pathogens, however, are patients that are immune-suppressed, have
open wounds or who are generally prone to illness at any age.

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The Benefit of Zoono
Any unprotected environment with surfaces (especially hospital environment), can be a
source of infection. Routine liquid disinfectants only work when the surface is wet. Once the
disinfectant is wiped off or dried, it no longer kills pathogens, hence the risk of re-
contamination of the disinfected surface by harmful microorganisms. In many cases this
occurs within 1-2 hours and the surface are contaminated to the same levels as prior to
disinfection.

Zoono technology is the next generation of biocide. It is water based and non-toxic and
therefore, does not requiring any special precautionary considerations as the more toxic and
corrosive biocides do.

“Normal disinfectants kill pathogens at point of use and are wet, once dry they
  no-longer work and many don’t achieve the contact time stipulated in their
laboratory tests – Zoono works when dry and continues to protect for up to 30
                           days and we can prove it”

Zoono Formulation
Zoono is a polymer that can adhere to any surface by covalent bonding, utilising silicon-
based polymers that form a monomolecular layer and combining this technology with a
quaternary ammonium biocide in aqueous solution. Once dry, the Zoono Z-71 becomes an
antimicrobial protective layer that remains active for up to 30 days, even after routine
cleaning applications. The layer uses a physico-chemical mechanism to draw
microorganisms onto the bonded polymer, which then disrupts and lyses the organism with
lethal effect. Unlike many other silicon formulations, Zoono is a clear liquid containing no
suspended polymer complexes that can reduce efficacy and block spray nozzles. The
formulation has also been enhanced by Zoono chemists to increase the binding capability to
surfaces and enhance stability.

 “The Zoono Z-71 formulation has been designed to increase surface bonding
                    and eliminate polymer precipitation”

The Proof
Zoono technology was tested using the most up-to-date laboratory tests carried out by a
UKAS accredited laboratory with years of experience in the testing of biocidal technologies.
The first tests carried out were the liquid and semi-liquid phase tests that are stipulated for
“wet” disinfectant products under “clean & dirty conditions”, all of which were successfully
passed.

   •   EN 1276 (bactericidal)
   •   EN 1650 (yeasticidal/fungicidal)
   •   EN 13697 (non-porous surface test in liquid phase, bactericidal)

The term liquid phase are tests carried out in test tubes. These test reports are available on
request.

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Residual Tests

                            Zoono Z71 Microbe Shield Residual Testing

                PAS 2424 is a test to measure residual efficacy over a 24-hour period, designed as close to
                the EN standards as possible and in use (in-vivo) conditions. The test is the most difficult to
                pass as a result of the abrasion steps that would remove non-residual biocidal technologies.

                The test involves applying the test organism to a steel disc and allowing it to dry. Test
                disinfectant is then applied and allowed to dry. Over a period of 24 hours the disc
                undergoes a series of abrasion cycles simulating cleaning and re-application of test
                organism to simulate recontamination of the surface. After 24 hours the disc is re-applied
                with the test organism and the surviving organisms counted. The final contact time being 5
                minutes.
  PAS 24/24
                Test organisms:

                     •    Pseudomonas aeruginosa
                     •    Staphylococcus aureus
                     •    Enterobacter hirae
                     •    Escherichia coli
                     •    Candida albicans

                             PAS 2424 - Result Z-71 – PASS

7 & 30 Day Test
After completion of the PAS 2424 testing the Z-71 trial continued on from 24 hrs to 30 days.
The table below demonstrates that there was still significant residual activity after 30 days.
In the absence of an official EN test there is no pass or fail criteria for residual antimicrobial
efficacy.

                               Zoono Z71 (Microbe Shield) Surface Biocide

     Test                                         Description and Explanation

                The EN 13697 test protocol requires the initial application of the test organism to a steel disc
                and application of the test biocide to establish the log kill (previously described).

                In the reverse engineered test, the biocide (Zoono Z71) is applied to the steel disc at the
                required concentration and allowed to dry and bond, providing the residual layer.
  Reverse
 Engineered     The test organism is then applied after the required time periods, in the case of the test 7
  EN 13697      and 30 days after application, to measure the stability of the mono-molecular layer and to
                establish if there was any antimicrobial activity.

                As this is not an official EN test, there is no official pass criteria

                Test organisms: Escherichia coli, Staphylococcus aureus

 Reverse Engineered EN 13697 (7 day) – both test bacteria 99.99%
        reduction after 7 days and 71 – 99.8 after 30 days.

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Conclusion
Zoono is a surface protection technology. It modifies surfaces to become antimicrobial
providing a long-lasting barrier to microbiological contamination, functioning and remaining
active when it has dried. Zoono technology cannot be compared to liquid phase or “wet
disinfectants” because it works differently. This is why there are currently no official
European residual biocidal efficacy tests.

    “Zoono provides an antimicrobial barrier that protects the environment
    continuously from harmful pathogens that could cause infection, “wet
 technologies only provide protection for a short period of time and surfaces
                         become re-contaminated”

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