Workprogramme EURL-Salmonella June 2017-2018 General discussion Closure - Kirsten Mooijman

Workprogramme EURL-
Salmonella
June 2017-2018
General discussion
Closure
Kirsten Mooijman

Interlaboratory comparison studies - Detection (I)
Up to and including 2016:
● Feb/March: interlaboratory comparison study on detection of
  Salmonella in samples from the primary production stage (pps)
● Sept/Oct: interlaboratory comparison study on detection of
  Salmonella in food or feed samples

!! Regularly problems with choice of pps matrix due to Avian Influenza
related to migration of wild birds in fall and winter
 Better to reverse the order of the studies, thus:
● Feb/March: interlaboratory comparison study on detection of
   Salmonella in food or feed samples
● Sept/Oct: interlaboratory comparison study on detection of
   Salmonella in samples from the primary production stage

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Interlaboratory comparison studies – Detection (II)

Problems when changing the order of the studies:
● Two studies with similar matrix organised closely after one and
  other
● In one year a study for either pps or food/feed will not be organised

Proposed solution:
Organise a study with a matrix which can be tested as ‘food sample’
and as ‘pps sample’

                Swabs

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Interlaboratory comparison studies – Detection (III)
 Swabs/sponges used for:
 ● Sampling after cleaning and disinfection in the primary production
   stage (ISO 13307)
 ● Carcass sampling (ISO 17604)
 ● Sampling of surfaces in the food industry environment and food
   processing plants (ISO 18593)

 EURL-Salmonella already performed some experiments (early 2017),
 by contaminating sponges with a mixture of Salmonella and
 ‘background flora’ (as possible alternative for chicken faeces)
  Good (stable) results

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Interlaboratory comparison studies – Detection (IV)
 Proposal detection studies:
 ● September/October 2017: Detection of Salmonella in swabs 
   open for NRLs-Salmonella for pps ánd food – use relevant method
   for own work field (for analysis pps samples or for analysis food).
   Indicate in reporting form if analysis done as NRL-pps or NRL-food
 ● February/March 2018: Detection of Salmonella in animal feed
 ● September/October 2018: Detection of Salmonella in samples from
   the primary production stage
 ● February/March 2019: Detection of Salmonella in a food matrix

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Interlaboratory comparison studies - Typing

● Organisation study on typing: October/November 2017
● For all participants: 20 (+1) serovars for serotyping
● Optional: PFGE for 10 serovars

From 2018 (??) replace PFGE or add: (??)
● MLVA typing of Salmonella Typhimurium (and Salmonella
  Enteritidis?)??
● Whole genome sequencing (WGS)??

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Summary results questionnaire MLVA (I)
● Most replies 26/01/2017 – 24/02/2017
● Sent to all NRLs-Salmonella (36 countries)
● Number of replies (countries): 26 (72%)

1. Do you perform MLVA for non-human isolates?
  No: 15 (CY, CZ, EE, FI, GR, LV, LT, NO, PL, PT, RO, SK, SI, ES, CH)
  Yes: 11 (AT, BE, BG, DK, FR, DE, IT, LU, NL, SE, UK)
  Note: 3 NRLs (EE, FI, NO) mentioned sending isolates to other
  (human health) institutes for MLVA typing e.g. in case of outbreaks
2. For which Salmonella serovars do you perform MLVA?
  Salmonella Typhimurium: 11
  Salmonella Enteritidis: 8
  Salmonella Dublin, Salmonella Derby: 1

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Summary results questionnaire MLVA (II)
3. Where do the isolates come from?
   Official controls: 9
   Outbreak investigations: 9
   Research: 9
4. MLVA protocol used for Salmonella Typhimurium?
   EFSA SOP (2014), incl. set of standardised strains: 4
   ECDC SOP (2011), incl. set of standardised strains: 7
5. MLVA protocol used for Salmonella Enteritidis?
   ECDC SOP (2011), incl. set of standardised strains: 8
6. Number of isolates typed with MLVA in 2016?
   0: 1                 500-1000: 3
   10-100: 4            >1000: 1 (including human isolates)
   100-500: 2

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Other activities Work programme 2017/2018 (I)
● Supporting activities:
  – Test different samples for interlaboratory studies
  – Ad hoc (e.g. confirmation of typing)
  – Support in outbreaks (e.g. typing strains)
● Activities for ISO/CEN:
  – Activities for (draft) standard on PCR for monoSTM
  – Project leader for development standard on validation of
    confirmation methods (EN ISO 16140-6)
  – Project leader for preparing guidance document for drafting
    standards for microbiology
  – Analysis results experiments incubation at 35/37 °C and conclude
  – Preparing draft manuscript validation study CEN mandate
  – Member of several (other) working groups

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Other activities Work programme 2017/2018 (II)

● Assistance to the Commission:
  – Ad hoc
  – Participation in working groups EFSA/DG-Sante
  – Support in case of outbreaks

● Communication:
  – Keep EURL website up to date
  – Newsletter every 3 months
  – Reporting results in reports (available at website) and
    when possible in publications

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Other activities Work programme 2017/2018 (III)
● Training
  – Upon request of NRL (e.g. for molecular typing) or indicated by
    EURL (in case of poor performance)
  – Use of BioNumerics, in cooperation with EURLs Listeria and STEC;
    4 NRLs per EURL network – Rome, July 2017 (NL 2018)
  – >2018: WGS? In cooperation with other EURLs (?)
● Molecular typing
  – Member of EFSA/ECDC steering committee molecular typing data
  – Curation of data (PFGE) for EFSA database
  – Curators meetings with EURLs Listeria, STEC and curator ECDC,
    to harmonise curation of data of EFSA/ECDC molecular database
  – Organisation interlaboratory studies for molecular typing
  – Use of WGS especially in outbreaks
  – Creation of a Working group on WGS of EURLs
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Location workshop 2018

                                               Uppsala, Sweden

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Other subject
● EURL-Listeria monocytogenes (Lm) prepared a guidance document
  ‘on outsourcing part of proficiency testing trials organised by NRLs
  for national networks’
● NRLs-Lm expressed a need for a guidance document, listing:
  – Criteria to outsource parts of PT schemes organised and
     supervised by NRLs for national networks;
  – Criteria to select PT providers, including steps of PT schemes that
     can be outsourced or not, frequency, details on method used by
     participants, together with minimum values if possible
● EURL-Lm proposed to make it a general guidance document for all
  biological EURL networks

 Need/interest NRLs-Salmonella in a (general) guidance document
for outsourcing part of PTs?

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Other subjects to discuss??

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Closure
● Presentations soon on website – allowed to publish all?
● Make sure you have signed the attendance lists
● Make sure you have checked the list with contact names and
  addresses of the NRLs
● Please complete evaluation form and hand over to EURL
● Make sure to send electronically completed (and signed)
  declaration form, together with boarding passes and all
  invoices by e-mail (scans), to EURL-Salmonella before 17
  June 2017
  Mind: no receipts  no reimbursement
● For the ones who gave a presentation(s): please send
  abstract(s) as soon as possible, before 13 June 2017
● Before leaving we will have lunch in the hotel

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THANK YOU!!
● European Commission
● Speakers
● Participants
● EURL-staff and secretariat

                           Safe journey home!

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