Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau
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Collaboration between
IRVT- OIE RL Brescia- Anses
Labib Bakkali, Stéphan Zientara, Pascal Boireau
ANSES
Animal Health Laboratory
Maisons-Alfort
Email: pascal.boireau@anses.fr
www.anses.fr
Maisons-Alfort Animal Health
Laboratory
Animal Health Laboratory
Created in1901
Bull.soc.fr.hist.méd.sci.vét., 2006, 6 : 79-107
virulentes, premiers vaccins à base de porteurs sains, règles de la prophylaxie
d’aphtes ou de tissus de cobayes sanitaire (Vallée et Carré), premier
neutralisés (Carré , Rinjard et Vallée : leurs
travaux ouvriront la voie au vaccin de
Schmidt et Waldmann ), hémo-prévention
(Rinjard), culture in vivo sur divers tissus
de fœtus de bovin (Thomas, Dhennin) ou
sur lapin nouveau-né (Verge, Dhennin,
Asso), vaccination par voie intradermique (
L.et L. Dhennin, Paraf, Larenaudie),
absence d’immunité croisée entre virus
OAC et SAT 1 (Santucci et coll.),
diagnostic et épidémiosurveillance
(Goureau, Kaiser, Moutou)
Fièvre charbonneuse : diagnostic,
surveillance, contrôle (Vaissaire).
Fièvre catarrhale ovine et Fièvre du Nil
occidental : surveillance de ces deux
Edmond Nocard (1900) Emile Roux
maladies, diagnostic et surveillance de la Henri Carré
maladie, enquêtes sérologiques évaluation Figure 2 : Henri Carré dans son
des vaccins (Zientara) laboratoire
Germes pyogènes : production de sérums diagnostic de l’avortement à virus de la
REFERENCE MISSIONS
Agrement Agriculture ministry, Health ministry, DG SANCO, OIE, FAO
2 European Reference laboratories
Brucellosis – Equine diseases
OIE reference laboratory - FAO Collaborative center
Brucellosis | Tuberculosis | EHDV
OIE Collaborative center
Food borne parasites
Human reference center
Brucellosis
REFERENCE MISSIONS
Agrement Agriculture ministry
2 LABORATOIRES
15 REFERENCE
DE national mandates
COMMUNAUTAIRE
Brucellose – Maladies équines
Anthrax,
Chlamydiosis
Brucellosis and tularemia
LABORATOIRE DE RÉFÉFENCE OIE - CENTRE
Tuberculosis
FMD COLLABORATEUR FAO
BluetongueBrucellose
and EHDV | Tuberculose | Paratuberculose
West Nile
Swine vesicular disease
Centre national de référence
Viral horse diseases and glanders, dourine (with
Brucellose
DOZULÉ Laboratory)
Centre national de référence associé
Parasite transmitted by food Trichinellosis,
Charbon bactéridien
Protozoa, Anisakis, (arthropod (ticks and
phlebotomes) borne diseases (Bartonella,
Anaplasma, Leishmania…))
Schmallenberg virus, Bunyavirus
Campus Alfort
New lab C3FMDV, tick
borne diseases « I3 »
CRBM
FMDV C3 lab
Training and international cooperation on
FMDV
Training and technology transfert
Research project for the
development of new tools
Networks REMESA and
RESOLAB
Bilateral actions on FMDV
Maisons-Alfort
18/10/10 to 29/10/10
- 2 training weeks
- Providing Reagents
Marocco Tunisia Algéria
Mustapha Soufien Nadera
HAMMOUCHI SGHAIER CHAOUACH
Workshop and training for FMDV diagnosis tests.
FAO Mauritanie
Tchad
Bénin Niger Tunisie Liban
Pakistan
Algérie
ANSES – Laboratoire de Santé Animale, Maisons-Alfort, France
21 – 25 May 2012
Training seminar Accra
Vet laboratory ACCRA (Ghana)
17 – 21 SEPTEMBER 2012
Kees van Maanen Labib Bakkali Kassimi
CAMEROON,
Dr. Simon Dickmu Jumbo
CENTRAL AFRICA REPUBLIC
Dr. Marie-Noel Ouaimon
COTE D’IVOIRE
Dr. Gnabro H. P. Godji
D. R.CONGO
Dr. Leopold Mulumba
GHANA
Dr. J. A. AWUNI
NIGERIA
Dr. Hussaini G. Ularamu
REPUBLIC OF CONGO
Dr. Ngakeni Faramalala
SENEGAL
Dr. Papa Serigne SECK
TOGO
Mr. Felix Kodzo WOUMETraining seminar on FMDV ACCRA: practical traning
Training for direct
indirect tests
LFD
Ag-ELISA & NSP ELISA
RT-PCRWorkshop on epidemiological surveillance of FMD and the
capacity of the laboratory
Dakar, 3-4 June 2014
Mali, Mauritania and Senegal
Project of common
epidemiological surveillance
among the three countriesMolecular epidemiology
Isolation and characterisation of FMDV strains
PhD Ghana Nigeria Cameroon
Benin
Type O Type O SAT 2 SAT 2
Type A
Gorna et al.
Vet.Microbiol. 2014
→ University of Liege, Belgium Accra Veterinary FMD Research Centre, Laboratoire
→ University of Abomey-Calavi Laboratory, National Veterinary National
in Cotonou, Benin Ghana Research Institute, Vétérinaire
→ Belgian Development Nigeria Cameroun
Collaboration Department
Institute of Tropical
Medicine (Antwerp)
CollaborationsBilateral actions on FMDV
Maisons-Alfort
16 juin 2014 au 27 juin 2014
- 2 weeks for training on emerging
outbreak
- To provide reagents
- Practical training in Tabat between
29/9 and 3/10/2014
Nadia CHAFFAI Amina AQARI
Biopharma
Rabat MaroccoPractical training in the field for FMDV
Stephan
ZIENTARA
Nakuru, Kenya 25-29 August 2014Support actions to REMESA for FMD
Workshops and Training
Bilateral action program of French
ministry of agriculture:
Welcome trainees
Training in countries by experts
Actions supported by EuFMD
Organization of workshop at
Maisons-Alfort
Participation to real time trainingSupport actions to Tunisia for FMD
Collaboration project between IRVT, OIE RL Brescia and Anses
IRVT IZSLER
Genetic and antigenic characterization of FMDV
strains circulating in Tunisia
Funded by:
French Ministry of Agriculture, Food and Forestry
Agency for the development of international cooperation in the areas of agriculture, food
and rural space (Adecia)
Instituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER)Context
FMD was introduced in
Tunisia in April 2014
First case was reported in
North East of country
29/4/2014 13/10/2014
Then, different outbreaks were
reported in different region
and in a short time
Virus identified : serotype O of WRLFMD
topotype Ind-2001dObjective
Investigation of FMDV evolution during the outbreak
Use of genome sequence and antigenic profiling data
to study:
dynamic of FMDV transmission
the virus matching with vaccine strains
Identify risk factors for the spread of the
disease in the countryPlanned activities
Action Month
IRVT will send samples to Anses 1
Welcome trainee from IRVT at Anses for 3 weeks 2
Analysis of samples by trainee : virus isolation, RT- 2 et 3
PCR, typing
Genome amplification of selected samples at Anses 4 et 5
Send isolats and amplicons to IZSLER 5
Full genome sequencing from amplicons by IZSLER 6à9
Antigen profiling of isolats by IZSLER 6à9
Data analysis 10-11
Repport 12Output Complete sequences of isolates of FMDV in Tunisia Phylogenetic analysis of sequences Antigenic characterization of isolates Analysis of epidemiological links between isolates based on phylogenetic analyzes Analysis of antigenic homology between the vaccine strains and isolates
Conclusion Global control of FMD needs coordinated action at national, regional and international level Collaborations between veterinary services and laboratories at regional and international levels are essential for the success of the control of FMD Anses participates and will continue to participate actively in the global fight against FMD In particular Anses-Animal health Lab, continues to provide support to REMESA members through training, expertise and collaboration project Collaboration projects such as that between IRVT, IZSLER and Anses should be encouraged
Thank you for your attention
“Unity makes strength”New diagnostic tools Improve the existing tests
Multiplex RT-PCR in one step for West Africa advantages: Detection and typing of four serotypes in one reaction Internal amplification control Cost
Duplex RT-PCR in one step.
advantages:
Simple (faster)
Less handling
Less risk of contamination
sensitive
IRES/β-actine
Internal amplification control
Or Stability Mastermix
3D/β-actine
20µl 5µlAlternative way for sample sendings
Inactivated
virus
Pen-Side test
advantages:
Less risk
Shipping Costs
Extraction
ARN
RT-PCR Transfection
VirusResearch project
Ensemble pour mieux comprendre
Identification and characterization of
circulating strains in West AfricaYou can also read
