CloneSelect Imager Objective, quantitative assessment of cell growth - Swapcard
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CloneSelect™ Imager Objective, quantitative assessment of cell growth
Assess cell confluence objectively and quantitatively
Rapid determination of the growth of cell lines is important for a number of processes,
such as optimization of cell culture conditions and verification of monoclonality.
However, conventional techniques are time-consuming, subjective and may risk interference with cell growth:
Tracking cell growth in 96-well plates is challenging and labor-intensive.
Produce consistent results – in less time
Save time and produce objective, quantitative, and consistent
results by using the CloneSelect™ Imager system to overcome the Confluence (%)
challenges associated with conventional techniques.
• Label-free white light imaging of living cells
• Suitable for adherent and settled suspension cells
• Growth rates accurately determined in every
well of a single-well or multi-well plate Time (hours)
Visual readouts for label-free, cell migration assays
• Determine migration rates, maximum migration rate and total migration area
• Screen one microplate within 3 minutes
• Easy to read, numerical and graphical output
0.0000 mm 2
0.6419 mm 2
1.2207 mm 2
0.0 hrs 6.1 hrs 23.9 hrs
Wound healing time course Wound healing heat map
Key benefits
• Assess cell confluence objectively and quantitatively
• Streamline workflow: image, analyze, report
• Image every well anytime to track colony formation and verify monoclonality
Track and record cell growth
CloneSelect Imager system estimates cell confluence and cell number
• Automatically scans every well in every plate • Enables viewing and tracking of every well in every plate
• Generates growth curves for each well • Reveals additional information on cellular morphology
and an understanding of growth characteristics
Sample by eye within Scan every well in every plate
wells across each plate
Conventional technique: subjective, time-consuming CloneSelect Imager: objective, automatic
Inconsistent results: cannot determine whole well confluence – Quantitative, whole well cell confluence for every well
well after well
Streamlined workflow: images, analyzes, reports
Imaging
Use adherent or settled suspension cells in microwell plate
CloneSelect Imager White light image
Optimize clonal outgrowth
The system is particularly useful for optimizing clonal outgrowth strategies when platform
approaches are not suitable e.g. when investigating new cell lines or variants.
Analysis Report
• Cell confluence and cell number estimation • Make confident, image-driven decisions
displayed for each well throughout plate history
• Growth curves calculated and displayed • Track and view growth of every cell line
Growth curves calculated and displayed
• Electronically track and store
plate data: cell confluence,
cell number estimation,
and growth curve
View every growth
Well selection Cell distribution curve in every well
is highlighted by
software overlay
01 02 03 01
04 02
05 03
06 04 05 06 01 02 03 01
04 02
05 03
06 04 05 06
3% Day 1 5% Day 2
A 36 38 A 31 36
29 3817 3118 29 17 18 A A
B1: 43% B1: 43% B1: 24390 B1: 24390
B 43 39 B 29 43
30 3919 2919 30 19 19 B B
C 43 38 C 36 43
31 3817 3618 31 17 18 C C
D 44 39 D 29 44
26 3917 2917 26 17 17 D D
E 44 35 E 25 44
27 3517 2517 27 17 17 E E
F 44 40 F 25 44
25 4015 2516 25 15 16 F F
G 48 38 G 29 48
27 3817 2916 27 17 16 G G
H 45 34 H 25 45
26 3416 2517 26 16 17 H H
15% Day 3 55% Day 7
Cell confluence & cell number estimation Repeated over
for each well displayed several days
Automate with robotic solutions
Electronic data tracking ensures control of high throughput processes
CloneSelect Imager integrated with robotics Process up to 75 lidded plates in a single run.
from Beckman Coulter. automate-it scara robot is recommended and supplied through Molecular
Photo courtesy of Beckman Coulter Corp., shows first generation Devices – optimized for CloneSelect Imager.
CloneSelect Imager system.
Verify monoclonality
After initial seeding, CloneSelect Imager system can image every well, at any time point,
using a ‘loci of growth’ functionality to highlight those wells that contain a single colony.
• Seed one cell per well and image at any point
• Focus on wells with a single loci of growth and view image history to verify monoclonality
• Verify colony origin by tracking image history of each well
Day 0 Day 3 Day 9 Day 11
Single loci
View image history Is colony
Yes! of colony development monoclonal?
Colony forming assay
After seeding in a matrix that enhances colony formation, such as a semi-solid media, cells are typically
incubated with compounds that may affect colony growth. The CloneSelect Imager system is used to image
every well to count colony number, estimate colony area and track colony growth.
• Image every well at any time point
• Analyze wells of interest e.g. showing inhibition of colony growth
• Export colony number and colony area for each well
Colony counting and colony area
“CloneSelect Imager has become an essential system for
verification of monoclonality within our cell line development workflow”
—Dr. Howard Clarke, Senior Staff Scientist in Process Development,
CMC ICOS Biologics Inc., USA
Optimize cell culture conditions
CloneSelect Imager has been used to rapidly screen culture variables to
identify optimal culture conditions for low density or clonal outgrowth.
• Identify low-cell density growth conditions over a two-week period
• Achieve a robust and extended growth range over base-case data
Base-case – Day 7 data.
Additional information gained on cellular morphology and understanding of growth characteristics.
Identify multiple nucleation points versus “edge only” growth Identify sub-optimal environmental conditions or “edge-effects”
Assess cell viability Accelerate cell line development
Replace cumbersome colorimetric MTT assays with a Monitor and evaluate outgrowth and productivity of cell lines that
non-invasive technique that enables monitoring over time* have been screened and selected using a ClonePix™ system.
• Direct overview of initial results per well
• Screen one microplate within 3 minutes
• Avoid costly colorimetric kits – no staining required
*Accurate non-invasive image-based cytotoxicity assays for cultured cells,
Marques-Gallego et al., BMC Biotechnology 2010, 10:43
“Maximize success rate for serum-free colony outgrowth in
chemically-defined media by prior optimization of growth conditions”
—Ben Hughes, Senior Bioprocess Engineer, NCRIS Biologics Facility,
Australian Institute for Bioengineering & Nanotechnology (AIBN), University of Queensland
Accelerate cell line development with a range
of Molecular Devices platforms
ClonePix 2 Mammlian Colony Picker
Automatically screen more clones in less time than conventional techniques, select cells
with optimal expression levels, and pick colonies with accuracy with the ClonePix™ 2 System.
ClonePix systems are now used in over 100 laboratories around the world to increase workflow
productivity, leaving more time to better characterize target proteins and run new projects.
QPix 400 Series Microbial Colony Picker
The QPix™ 400 series of microbial colony pickers offer you the unique option to simultaneously
detect colonies and quantify fluorescent markers in a prescreening step before picking.
Our QPix systems are used worldwide in over 600 installations in research institutes,
biotech, and pharmaceutical companies. QPix robotics developed a famous reputation
for reliability and accuracy in sequencing centers during the Human Genome project.
SpectraMax i3x Multi-Mode Microplate Reader
The SpectraMax® i3x multi-Mode microplate reader measures spectral-based
absorbance, fluorescence, and luminescence with the added functionality of
modular upgrades for western blot, imaging, and fast kinetics with injectors.
Unrivalled solutions based on excellent imaging and intelligent image analysis Products from Molecular Devices offer scientists unrivalled solutions that utilize imaging and intelligent image analysis to support basic research, pharmaceutical and biotherapeutic development. The company’s systems continue to establish industry standards in areas such as picking microbial colonies for genomic studies or screening and selection of mammalian cell lines. Other systems use imaging platforms to monitor cell growth, evaluate cellular responses and quantify protein production. Through its expertise in robotics, cell and molecular biology, image analysis and interpretation, supported by a strong IP portfolio, the company is committed to the continual development of innovative solutions for life science applications. CloneSelect Imager system specifications Imaging Software Dedicated imaging software pre-installed on a high specification PC, Microsoft Windows 7 White light imaging Trans-illumination Data tracking 1 x internal barcode reader for data tracking of each plate Camera High-resolution CCD camera Imaging speed 96-well microplate: 90 sec Objective 4x Resolution Standard: 3.6 micron; Maximum: 1.8 micron Instrumentation Source plate type Range of 6-, 12-, 24-, 96-well SBS microplates Source plate capacity 1 x plates Instrument dimensions 720 mm (width) x 428 mm (height) x 575 mm (depth) Instrument weight 45 kg Regulatory approval Compliance CE Quality ISO9001:2008 certified Automation compatibility API suite available for robotic integration. Please contact us for details. For more information, visit www.moleculardevices.com. Contact Us Regional Offices Phone: +1.800.635.5577 USA and Canada +1.800.635.5577 China (Beijing) +86.10.6410.8669 Japan +81.3.6362.9109 Web: www.moleculardevices.com United Kingdom +44.118.944.8000 China (Shanghai) +86.21.3372.1088 South Korea +82.2.3471.9531 Email: info@moldev.com Europe* 00800.665.32860 Hong Kong +852.3971.3530 Check our website for a current *Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Netherlands, Spain, Sweden and Switzerland listing of worldwide distributors. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. ©2020 Molecular Devices, LLC Specifications subject to change without notice. Patents: www.moleculardevices.com/productpatents 8/20 1941E FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Printed in USA
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