Thrive Bioscience Analytics. Automation. Better Biology - Introduction to Company & Products February 22, 2021 - RESI Longevity
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Thrive Bioscience
Analytics.
Automation.
Better Biology.
Introduction to Company & Products
February 22, 2021
“50 Fastest Growing Companies of 2020” -- Silicon Review
“20 Best Biotech Companies of 2019” -- CIO Review
© Thrive Bioscience, Inc. 2021
Introduction to Thrive
© Thrive Bioscience, Inc. 2021 2
Good science needs good cells!
Current Status of Cell Culture:
• Cell culture is at the center of bio-medical research -- 1952 - 2020
$22 billion per year market.
• Cell culture is conducted similarly to 65 years ago --
significant unsolved pain points and limited innovation.
• Cells vary significantly in quality across labs & experiments --
high rates of mislabeling, cross-contamination and stress.
Result:
Billions of dollars are spent each year on assays and instruments
to study cells of variable and often poor quality. 2020 +
Thrive Bioscience’s Solution:
Instruments and software that provide imaging, analytics, and
automation for reproducible cell culture.
Thrive Instruments
© Thrive Bioscience, Inc. 2021 3
Thrive Bioscience -- Key Points
ü Commercialization of Family of Instruments and Software -- for small, medium & large labs
with common database and analytics
o CellAssist launched late 2020 (12 sites in US, Canada, Japan and Korea)
CellAssist o CellAssist 50 started shipping December (2 sites)
(2020)
o Hired Commercial Team August to September
ü Strategic Collaborations --
o Collaboration with $18 billion big pharma for development of versions of Thrive instruments for
bioprocessing & cell therapies
o Academic collaborations for software applications with Harvard Stem Cell Institute, Massachusetts
General Hospital Center for Genomics Medicine and University of Texas Medical Galveston
CellAssist 50
(2021) ü Limited Competition / Extensive IP Portfolio -- 64 applications (of which 14 issued) covering
cell, tissue & pathogen culture, IoT, blockchain, and AI for research and bioprocessing
ü $25.4 Million of Financing Raised To-date --
o Family offices (Lauder Partners, Milstein Family, Parker Bros Family)
o Angel groups (Landmark Angels, Life Science Angels, SideCar Angels)
o
o Industry executives (former CEO’s of Novartis, Cytyc, Exact Sciences, Brammer Bio, Patehon)
Cell Culture
Management System
(2023) ü Financing -- Series B -- $12M total, starting initially with $2M convertible note to close in April; use of
proceeds for sales expansion, development of software applications, and 3rd instrument engineering
© Thrive Bioscience, Inc. 2021 4
Leadership Team
Thomas Forest Farb-Horch o President and COO of Indevus Pharmaceuticals (acquired by Endo)
President , Co-Founder and o VP of Corporate Development of Cytyc (acquired by Hologic)
Board Director
o Founder / Board Member of HNC Software (neural networks/acquired by Oracle and Fair Isaac),
Exact Sciences (NASD: EXAS)
Alan-Philippe Blanchard o Founding Member of Rosetta Inpharmatics (acquired by Merck)
Ph.D., CSO o Research Fellow, Life Technologies/Thermo Fisher
Co-Founder and Board
o Senior Fellow, Leroy Hood Laboratory, University of Washington
Director
Taka Kiyoizumi, M.D., Ph.D. o Founder and CEO, MediciNova (NASDAQ: MNOV)
EVP of International o President and CEO, Tanabe Research Laboratories, USA, US affiliate of Mitsubishi Tanabe Pharma
Herb Kenny, o VP of Commercial Operations, Scientific Instruments, Thermo Fisher ($2 billion of sales)
EVP of Commercialization o 34 years in global sales, service and marketing experience
Jeff Goldman, M.S. o R&D Manager, Seahorse Bioscience (acquired by Agilent)
VP of Engineering and o Engineering Director, 454 Life Sciences (acquired by Roche)
Manufacturing
o Product Development Director of MJ Research (acquired by Bio-Rad)
© Thrive Bioscience, Inc. 2021 5
Outside Board of Directors
Guy Broadbent o Former President, Thermo Fisher Scientific, Laboratory Products Division
Chair of Board o Former CEO, XcellereX (acquired by GE Life Sciences) ns Argotec (acquired by
Schweitzer-Mauduit)
o Board Member at Advanced Instruments, Aquapak Polymers (Chair), Aurora
Plastics and Clock Spring|NRI (Chair)
Michael Finney, Ph.D. o Managing Director of Finney Capital and active Member of Life Science Angels
(MIT, Biology) o Former CSO, MJ Research (acquired by Bio-Rad) and CEO, Vaxart
o Board Member at The Genome Partnership, Innerscope Research, Orion
Genomics, Sage Science (Chair) and Vaxart
Brock Reeve, MPhil (Yale), o Executive Director, Harvard Stem Cell Institute
MBA (Harvard) o CEO of IVIVA Medical
o Former Managing Director and COO of Life Science Insights, an IDC company
o Former Healthcare Practice Director of Viant and Principal of SRI Consulting
© Thrive Bioscience, Inc. 2021 6
Thrive’s Solution -- Family of Cell Culture Instruments & Software
Integrates imaging, robotics, fluidics & machine learning
to grow high quality adherent cells consistently
o Common database, image processing & modules across products
o No FDA clearance or approval required
CellAssist CellAssist 50 Software Modules Cell Culture System
On Market Early Access On Market Available 2021 - 2023 Available 2023
Bench-top imaging & 24/7 Automated CellAssist for Stand-Alone Application Walk-away intelligent 100
analytics 50 plates Packages Solving Major plate cell culture
Problems in Cell Culture
• 12 Customer & • 2 Customer & Collaborator Sites • Organoids & 3D Culture • In alpha test
Collaborator Sites • Integrates the CellAssist,
• Single Cell & Single Colony • 1,050,000 list (U.S.
robotics, & an automated
incubator Tracking
• $95,000 list (U.S.)
• $275,000 list (U.S.) • Infectious Disease Rapid
Viral Plaque Assays
• Stem Cell Differentiation
(.5m x .5m x .6m) 7m x .7m x 1.7m Detection & Monitoring 2.4m x 1.0m x 1.8m
© Thrive Bioscience, Inc. 2021 7
CellAssist Family -- The Right Data and Lots of Data for Cell Biology
Data.
Analytics.
Analytic Documentation.
Workstation Reproducible Cell Culture.
in Lab or Office
Image Collection Example
6-Well Plate at 4x
• 1,470 images in 10 mins
ü 7 z-heights @ 20 µm increments
ü 35 image tiles per well at each z-
CellAssist Imager in Lab height
• 6-well to 96-well plates ü 6 wells
• 4x & 10x magnification
• Phase contrast and • Image Registration
bright-field modes ü 15 microns between scans (after
• Single and 50-plate removing and reloading plate)
versions
• Genomics Quantity of Data Now
Available for Cell Biology
ü 7.4 Gb data-set per plate scan
ü 1.0 TB per month for many labs
© Thrive Bioscience, Inc. 2021 8
Example Software Applications for CellAssist Family
Viral Plaque Assays:
Examples of software modules to be sold in
addition to the instruments include:
Ø Viral plaque assays (plaques visible in 24 to 36
hours)
Ø Organoid /spheroid imaging
Clonality Determination:
Ø Automatic determination of monoclonality or
polyclonality of stem cell colonies
Ø Automatic tracking of single colonies or cells
Organoid / Spheroid Imaging: Single Colony & Single Cell Tracking:
Day 2 Day 4 Day 6
#1: 24-Dec-2018 #2: 31-Dec-2018 #3: 07-Jan-2019 #4) 14-Jan-2019 #5) 22-Jan-2019
© Thrive Bioscience, Inc. 2021 9
CellAssist 50 Screen Shot (example of imaging every four hours)
© Thrive Bioscience, Inc. 2021 10CellAssist Family Capabilities & Benefits
Analytics. Automation. Better Biology.
ü Fits into current daily lab workflow when examining cells
ü More thoroughly images & inspects cells & identifies
problems
ü Automatically documents what is rarely captured
currently -- who, what, when, where, how
ü Builds a database of cells, experiments and results
ü Enables labs to review & verify within & across labs
CellAssist significantly improves many
workflows from research to bioprocessing
Basic Pre-Clinical Pilot Process Yield & Quality
Research Development Production Optimization Cost
© Thrive Bioscience, Inc. 2021 11Cell Culture Market Size and Applications of Thrive Instruments
Cell Culture -- $18.6 Billion Market Per Year in 2020 Worldwide
• 72% Pharma/Biotech/Cosmetics/Industrial
• 28% Research / Academic Labs
Relevant Fields of Life Sciences Relevant Uses in Life Sciences
Virus Research
(CoV, RSV, HIV, Zika)
CellAssist Enables
Vaccine • Precise measurements of Immuno-
Research cell characteristics therapy
• Engineered cells
Cancer Drug
Research • Autologous therapies Discovery
• Building multi-cell
Artificial structures Stem Cell
Organs Research
Regenerative Screening on
Medicine Cell Lines
© Thrive Bioscience, Inc. 2021 12Example Customer
Applications
© Thrive Bioscience, Inc. 2021 13Customer Application:
Analysis of Outliers to Improve Quality
CellAssist improves quality and documentation of cells
Problem: Current tools do not collect
images and data along with tools that
enable users to visualize outliers. Mislabeled Cell Culture Plates
Outliers are important because they
may indicate problems in quality that
require investigation.
Objective: Document and analyze
images and data on cells over time to
improve quality and reproducibility of
cell culture.
CellAssist Solution: CellAssist
provides comparable data over time
that allows for identification of
outliers.
(In this example, upon further
investigation, the customer found
(statistical anomaly highlighted)
cases of mislabeling.)
© Thrive Bioscience, Inc. 2021 14Customer Application:
Confluence in Center and Outer Rings
CellAssist provides objective metrics and images for consistent decision-making
Problem: Individuals vary in their
determination of confluence. Further,
particularly when using bright-field,
they may ignore the outer ring of the
wells.
Objective: Standardize measurement of
confluence.
CellAssist Solution: CellAssist
objectively measures confluence in the
center and more difficult to read outer
ring of the well.
© Thrive Bioscience, Inc. 2021 15Customer Application:
Single Colony Tracking & Monoclonality Probability Determination
CellAssist improves quality and increases yields by using data to grade and pick colonies
Problem: Gene editing transfection rates can be poor, so it is
important that monoclonal colonies be selected for passaging.
Objective: Accurately determine whether a colony is monoclonal
by capturing series of time lapse images with excellent
registration across scans.
CellAssist Solution: The CellAssist time-lapse imaging enables
the lineage of candidate colonies to be tracked to a single starting
cell.
See video of time lapse images that allow researchers
to select monoclonal or polyclonal colonies:
Day 2 Day 4 Day 6 https://vimeo.com/348386015/dc51c9038c
© Thrive Bioscience, Inc. 2021 16Customer Application:
Comparison of Cell Growth on Various Plate Surface Coatings
CellAssist analyses provide optimization, yield enhancement , and cost savings
Problem: (a) The coating of plates can profoundly affect the survivability and growth of colonies (in this case gene edited ones starting
with very small quantities). (b) Some coatings are also significantly more expensive.
Objectives: Compare two coatings to choose the optimal one by characterizing and comparing the cells under both conditions over
time. (b) Tailor, test and directly compare the impacts of new protocols to select the best coating AND protocol.
CellAssist Solution: The CellAssist’s documentation, colony counting curves and examination of images are used to characterize the
number of colonies for each condition, and the results are collated and easily compared across conditions.
© Thrive Bioscience, Inc. 2021 17Customer Application:
Time-Stamped Barcode Documentation for Reproducible Cell Culture
CellAssist automatically tracks cells, metrics, plates and reagents throughout the life cycle of a project
© Thrive Bioscience, Inc. 2021 18Analytics. Automation. Better Biology.
Contact:
Thomas Forest Farb-Horch, CEO
tom@thrivebio.com
Thrive Bioscience, Inc., 11 Audubon Road, Wakefield, MA 01880
USA
© Thrive Bioscience, Inc. 2021 19You can also read
