NEWS 2020 Life Science - Carl Roth
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NEWS 2020
Life Science
NEW Products and Programme Extensions
Life Science
Table of contents
Electrophoresis - PAGE Precast Gels p. 2
Electrophoresis - Marker p. 3
Electrophoresis - Tank-Blotting p. 4
Electrophoresis - Agarose/Accessories for Units p. 5
Electrophoresis - Gel Documentation p. 6-7
Molecular Biology - Enzymes p. 8-10
Microbiology - Agar/Granulated Media p. 11
Histology - Reagents p. 12
Electrophoresis · PAGE Precast Gels
Q-PAGE™ Precast Gels Store at +4 °C
Transport temp.: cooled
The proven GoPAGETM TGN precast gels under a new name in a new design and with
optimized separating properties:
• Even faster separation - clear, sharp bands already after 30-40 minutes
• Wide separation range - available as homogeneous gels (10 %) and adjusted gradient gels
(4 to 15 %) in 12- and 15-well formats
• Size of the gel cassettes - Mini (10 x 8,3 cm) and Midi (10 x 10 cm), suitable for most mini-gel tanks
• Particularly long shelf life - up to 12 months from date of manufacture when stored at 4 °C
• Easy to monitor during the run - transparent reference lines on the gel cassette
• Easy to load samples - numbered wells with separators
NEW Products and Programme Extensions
Compatibility:
Q-PAGETM TGN Mini (10 x 8,3 cm):
• Bio-Rad® Mini PROTEAN® Tetra Cell
• Chambers of similar size of other manufacturers
Q-PAGETM TGN Midi (10 x 10 cm):
• Roth PROclamp Mini (Art. No. 3501.1-3503.1)
• Roth Mini Vertikal (Art. No. Y001.1-Y004.1)
• Invitrogen® XCell SureLock® Mini-Cell
• Invitrogen® Mini Gel Tank
• Hoefer SE260 Solid blister tray for safe transport of the gels.
• Chambers of similar size of other manufacturers Gel cassettes with comb to protect the gel pockets.
Q-PAGETM-Precast Gels
Gels are supplied shrink-wrapped in Tris/Glycin buffer (preserved).
Q-PAGETM TGN MINI 10 x 8,3 cm
Product Wells Max. vol. / well Buffer system Gel strength Gel plates (W x L) Gel size (W x L) Art. No. Pack Qty. €
Q-PAGE™ 10 % TGN Mini 3681.1 2 gels 39,90
12 25 μl Tris/Glycin w/o SDS 1 mm 10 x 8,3 cm 8,3 x 6,5 cm
Continuous matrix for proteins of 25 to 200 kD 3681.2 10 gels 169,00
Q-PAGE™ 10 % TGN Mini 3682.1 2 gels 39,90
15 22 μl Tris/Glycin w/o SDS 1 mm 10 x 8,3 cm 8,3 x 6,5 cm
Continuous matrix for proteins of 25 to 200 kD 3682.2 10 gels 169,00
Q-PAGE™ 4-15 % TGN Mini 3670.1 2 gels 39,90
Matrix in Gradient for proteins of 5 to 200 kD 12 25 μl Tris/Glycin w/o SDS 1 mm 10 x 8,3 cm 8,3 x 6,5 cm
3670.2 10 gels 169,00
Q-PAGE™ 4-15 % TGN Mini 3671.1 2 gels 39,90
15 22 μl Tris/Glycin w/o SDS 1 mm 10 x 8,3 cm 8,3 x 6,5 cm
Matrix in Gradient for proteins of 5 to 200 kD 3671.2 10 gels 169,00
Q-PAGETM TGN MIDI 10 x 10 cm
Product Wells Max. vol. / well Buffer system Gel strength Gel plates (W x L) Gel size (W x L) Art. No. Pack Qty. €
Q-PAGE™ 10 % TGN Midi 3683.1 2 gels 39,90
Continuous matrix for proteins of 25 to 200 kD 12 40 μl Tris/Glycin w/o SDS 1 mm 10 x 10 cm 8,3 x 8,1 cm
3683.2 10 gels 169,00
Q-PAGE™ 10 % TGN Midi 3684.1 2 gels 39,90
15 28 μl Tris/Glycin w/o SDS 1 mm 10 x 10 cm 8,3 x 8,1 cm
Continuous matrix for proteins of 25 to 200 kD 3684.2 10 gels 169,00
Q-PAGE™ 4-15 % TGN Midi 3673.1 2 gels 39,90
12 40 μl Tris/Glycin w/o SDS 1 mm 10 x 10 cm 8,3 x 8,1 cm
Matrix in Gradient for proteins of 5 to 200 kD 3673.2 10 gels 169,00
Q-PAGE™ 4-15 % TGN Midi 3676.1 2 gels 39,90
Matrix in Gradient for proteins of 5 to 200 kD 15 28 μl Tris/Glycin w/o SDS 1 mm 10 x 10 cm 8,3 x 8,1 cm
3676.2 10 gels 169,00
For further information and detailed instructions-for-use see carlroth.com ▶ Life Science ▶ Electrophoresis ▶ PAGE
2 Carl Roth GmbH + Co. KG · Schoemperlenstr. 3-5 · 76185 Karlsruhe · www.carlroth.com · Phone +49 721/5606 510 · Fax +49 721/5606 111
Electrophoresis · PAGE
Markers
Marker TRICOLOR
Art. No. 8271 (fig. 3)
Size Range (kD) 10 - 245
Number of Fragments 12
Reinforced band at (kD) -
Quantitation not recommended
Staining blue prestained
Additional reference bands 25 kD green, 75 kD red
Detection in Western-Blot no detection
Number of Lanes in minigel 200 / ml
Delivery ready-to-use
(kD)
NEW Products and Programme Extensions
- 245
- 180
- 140
- 100
- 75
- 60
- 45
- 35
- 25
- 20
- 15
- 10
Roti®-Mark TRICOLOR
Protein marker, prestained
Size range: 10-245 kD
Number of protein bands: 12
Number of lanes/ml: approx. 200 (mini gels)
Storage temp.: -20 °C • Transport temp.: room temp.
Protein marker including tricolour-prestained proteins of 10 up to 245 kD. In
order to visualise separation during the gel run and for better orientation in
the gel, two proteins of approx. 25 and 75 kD have been prestained in green
and red, respectively, in addition to the blue coloured bands. All proteins
excel by having a very even running behaviour and form distinct bands. The
marker is designed as ready-to-use solution for direct application to the gel.
Fig.: 4-20 % gradient SDS-PAGE (Tris)
8271.2 250 μl New size plastic 89,50 €
8271.1 2 x 250 μl plastic 165,00 €
Please note: Reliable size determination of proteins cannot be performed using prestained markers. For this,
we recommend the use of our unstained marker Roti®-Mark 10-150 PLUS (Art. No. X879) with one prestained protein.
For ordering and information about prices and delivery in your country please contact us or your local distributor · info@carlroth.com 3
Electrophoresis · Blotting
Tank Blotting
NEW Products and Programme Extensions
Rotiphorese® PROclamp MINI Wide
Tank Blotting System
Maximum blotting area: 4 gels of up to 20 x 10 cm
• Suitable for all gels of maximum 20 x 10 cm
• Compatible with the Rotiphorese® PROclamp MINI Wide unit
for vertical electrophoresis (Art. No. 1395.1)
The tank blotting system Rotiphorese® PROclamp is ideally suited for the
gentle transfer of peptides and proteins of any size. It is ideal for large,
temperature sensitive or native proteins.
Delivery incl. 1 Rotiphorese® PROclamp MINI Wide buffer tank, 1 safety
lid with cables and 1 PROclamp MINI Wide blotting module for max. 4 gels,
incl. 4 cassettes and 16 fibre blotting mats, 1 cooling pack.
Art. No. Pack Qty. €
Compatible with the vertical electrophoresis system
3697.1 1 unit 899,00 Rotiphorese® PROclamp MINI Wide (Art. No. 1395.1)
PROclamp MINI Wide Blotting Accessories
Type Art. No. Pack Qty. €
PROclamp MINI Wide tank blotting module
3699.1 1 unit 459,00
(incl. 4 cassettes and 16 blotting mats)
PROclamp MINI Wide compression cassette
3702.1 1 unit 53,60
(incl. 2 blotting mats)
PROclamp MINI Wide blotting mats 3654.1 8 mats 52,00
4 Carl Roth GmbH + Co. KG · Schoemperlenstr. 3-5 · 76185 Karlsruhe · www.carlroth.com · Phone +49 721/5606 510 · Fax +49 721/5606 111
Electrophoresis · Agarose/Accessories for Units
Now in blister pack!
Typical PROfessional runVIEW gel: Marker DNA with EvaGreen® under
blue light. Picture taken during the run. 100 bp Ladder, pBR328 Mix I and
1 kbp Ladder (1,2 µg each) in 1 % agarose / 0.5x TBE.
Agarose-Tablets
Roti®garose 0,5 g agarose/tablet
Standard agarose with low EEO, for routine analyses (≥300 bp).
• Easy-to-use and safe tablets for constant gel strength and
well reproducible gels
• Reliable agarose for routine analyses
• For detection of inserts or PCR amplificates of over 300 bps
NEW Products and Programme Extensions
• Agarose with high gel strength, applicable from 0,5 to 2,5 %
• Compatible with all standard running buffers and nucleic acid-staining
methods
1 % agarose in 0.5 x TBE Rotiphorese® PROfessional runVIEW lid
Special lid for real-time electrophoresis with the runVIEW system.
kb kb
The lid has a built-in fan and an integrated spectral emission filter for blue
10 - light, which is suitable for red and green fluorescence. Together with the
4-
-5 runVIEW system they make stained DNA visible during the run.
-3
2- -2 Type Use Accessories for Art. No. Pack Qty. €
for unit 9958.1 (runVIEW
PROfessional I Base MINI I) 3663.1 1 unit 185,00
1- -1
for unit red and green 9961.1 (runVIEW
PROfessional II fluorescence Base MINI II) 3680.1 1 unit 189,00
0.6 -
- 0.5 for unit 4580.1 (runVIEW
PROfessional III Base) 3077.1 1 unit 145,00
0.2 -
- 0.25 Further information on runVIEW is available on the Internet under
Art. No. 4849.1
Guarantee analysis:
Electroendosmosis (EEO). ..............................................................................0,05-0,1
Water content. ....................................................................................................≤10 %
Sulphate (SO4). .................................................................................................
Electrophoresis · Gel Documentation
Accessories Felix Gel Documentation Systems
NEW Products and Programme Extensions
Basic ImageSystem Felix 3000
By Biostep. Basic package for gel documentation, consisting of Felix 3000
SLR camera with filter (EtBr or optional), biostep argusX2® basic control
software and biostep argusX2® camera control module.
Minimum requirements for PC: Pentium/AMD 1.8 GHz, 2 GB, Win 7/8/10, 2-
3 free USB ports.
System features:
• Very fast, lag-free live image
• Automatic focussing
• High resolution
• Very good detection sensitivity
• Dynamic range up to 16 Bit (greyscales) due to software addition
• Connection of different lenses (selection depending on application)
Technical data camera:
Type High-resolution reflex camera
Lens Manuel zoom lens F3.5
Sensor CMOS (22,3 x 14,9 mm)
Resolution 24 megapixels (6000 x 4000)
Greyscale 8 bit (256)
Colours 48 bit (16,7 x 106)
Computer interface USB 2.0 port to control via argusX2®
Weight approx. 580 g
Delivery incl. SLR camera, 1 filter (EtBr 600 nm or optional), control cable,
biostep argusX2® basic control software and biostep argusX2® camera
control module. Filters for Felix systems
Please order transilluminator and dark hood separately if required. Type Wavelength (nm) Art. No. Pack Qty. €
Art. No. Pack Qty. € Red light filter 600 1749.1 1 485,00
3696.1 1 2595,00 Green light filter 530 2104.1 1 489,00
6 Carl Roth GmbH + Co. KG · Schoemperlenstr. 3-5 · 76185 Karlsruhe · www.carlroth.com · Phone +49 721/5606 510 · Fax +49 721/5606 111
Electrophoresis · Gel Documentation
Accessories Felix Gel Documentation Systems
Set-top Dark hood DH-10
By Biostep.
• For placing over a transilluminator
• Compatible with various transilluminators of different producers up to
(W x D) 30 x 23 cm filter size
• Bracket for cameras, max. camera weight: 1 kg
• Suitable for the image documentation outside the dark hood,
e.g. with the Basic ImageSystem Felix 3000 (Art. No. 3696.1)
Technical Information:
Type DH-10
Footprint (W x D) 30 x 25 cm
Height 37 cm (without camera bracket)
Art. No. Pack Qty. €
NEW Products and Programme Extensions
3695.1 1 295,00
Analysis software CLIQS, single license
By Biostep. Affordable analysis software with modules for evaluation of 1D
gels, blots, array images, colony counting and toolbox for non-specific
evaluations. Can be used under the operating systems Win 7, 8, 10.
Software functions:
• Import of files of all common image formats (Tif, jpeg, bmp, png etc.)
• Export data to other Windows applications
• Labeling and printing functions
• Extensive options for displaying the evaluation results
Modules:
• Evaluation of 1D electrophoresis gels, blots
• Evaluation of dot/slot blots, microtiter plates and arrays
• Colony counting and spot detection
• Evaluation of TLC plates, autoradiographies and unspecific structures
Type Art. No. Pack Qty. €
for gels, blots, arrays, colonies, TLC 3694.1 1 999,00
For ordering and information about prices and delivery in your country please contact us or your local distributor · info@carlroth.com 7
Molecular Biology
Enzymes for DNA/RNA Isolation
Ribonuclease H Proteinase K DNAse-/RNAse-free
5 U/µl, for Molecular Biology lyophilised, ≥35 U/mg, BioScience-Grade
For hydrolytic cleavage of phosphodiester bonds between RNA and DNA. High-quality endopeptidase for molecular biology. Isolated from fungi
Storage temp.: -20 °C • Transport temp.: cooled (Tritirachium album).
Non-specific endoribonuclease that specifically cleaves RNA into Storage temp.: -20 °C • Transport temp.: cooled
RNA:DNA hybrids. At least four continuous base pairs (RNA:DNA) n g Danger H315-H319-H334-H317-H335
are required for activity. The RNase H cleaves RNA to release
5´ oligoribonucleotides. Endopeptidase for the degradation of proteins with very high solubility,
RNase H does not degrade single and double-stranded DNA specific activity and DNA purity.
or unhybridized RNA.
The kit consists of color coded tubes for easier identification to avoid • High solubility
confusion of reagents. • High specific activity
• Extremely low DNA content
The kit contains:
RNase H Proteinase K (from Tritirachium album) is a non-specific protease of the
Reaction buffer (10x) serine protease family. Proteinase K is used for the cleavage of proteins in
The individual components of this kit cannot be purchased separately. nucleic acid preparations. It is mainly used in nucleic acid purification or for
250 U correspond to 50 preparations with a volume of 20 µl. the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including
NEW Products and Programme Extensions
Type analysis: elevated temperatures and presence of SDS.
Purity. .................................................................................................................>90 %
Concentration. .................................................................................................... 5 U/µl Activity: ≥35 U/mg lyophilizate
DNase activity...........................................................................................................no Specific activity: ≥45 U/mg protein
DNA content: ≤0,1 pg/mg
3728.1 250 U plastic 165,00 € Foreign activity: RNase and DNase not detectable
Optimum temperature: +65 °C.
3728.2 1250 U plastic 669,00 € Activity at +65 °C is approx. 12 x higher than at +25 °C.
Over +65 °C, inactivation due to denaturation.
Stability: pH 4,0-12,5
Also stable even when denaturing agents, e.g. SDS and urea are present.
Activators: Denaturating agents (SDS, urea).
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol.
Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotrypsin
inhibitors.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.
Application:
Reaction buffer: 50 mM Tris-HCl; pH 7,5; 5 mM CaCl2; 0,5 % SDS
Stock solution: 20 mg/ml in water
Storage temperature (stock solution): -20 °C
Working solution: 50 µg/ml
Guarantee analysis:
Appearance. ............................................................................................white powder
Activity. ......................................................................................................... ≥35 U/mg
Specific activity. ............................................................................................ ≥45 U/mg
Solubility in H2O.......................................................................................... ≥50 mg/ml
DNA content. ..............................................................................................≤0,1 pg/mg
Exonucleases. ....................................................................................... none detected
Endonucleases. ..................................................................................... none detected
Ribonucleases....................................................................................... none detected
3726.1 100 mg glass 145,00 €
3726.2 1g glass 690,00 €
8 Carl Roth GmbH + Co. KG · Schoemperlenstr. 3-5 · 76185 Karlsruhe · www.carlroth.com · Phone +49 721/5606 510 · Fax +49 721/5606 111
Molecular Biology
Enzymes for DNA/RNA Isolation
S DNAse-/RNAse-free Protease TEV
Proteinase K - Solution 1 U/µl, for Molecular Biology
20 mg/ml Highly site-specific cysteine protease from Tobacco Etch Virus.
Non-specific protease for degrading proteins in biological samples. Isolated Storage temp.: -20 °C • Transport temp.: cooled
from fungi (Tritirachium album). Suitable for the removal of fusion marks from recombinant proteins after
Storage temp.: -20 °C • Transport temp.: cooled protein purification.
n Danger H317-H334 • For removal of affinity tags from fusion proteins
• Specific activity: 1 U/µl
• Enhanced enzyme stability
• Sterile ready-to-use solution • Activity over a broad range
• Specific activity: ≥800 U/ml
• Stable over a wide pH range: 4,0-12,5 Recombinant TEV Protease is a highly site-specific cysteine protease,
• Active at high temperatures and denaturing conditions which is found in the Tobacco Etch Virus. Due to its sequence specificity,
the TEV protease is a very powerful reagent for the removal of fusion tags
Proteinase K (from Tritirachium album) is a non-specific protease of the from recombinant proteins after protein purification. TEV Protease specifi-
serine protease family. Proteinase K is used for the cleavage of proteins in cally recognizes a seven amino acid sequence of the general form Glu-X-X-
nucleic acid preparations. It is mainly used in nucleic acid purification or for Tyr-X-Gln↓ (Gly/Ser). Most commonly Glu-Asn-Leu-Tyr-Phe-Gln↓ Gly and
the removal of nucleases. cleaves between glutamine and glycine or serine.
NEW Products and Programme Extensions
Guarantee analysis:
Appearance. ................................................................................clear, colorless liquid The kit contains:
Concentration. ............................................................................................... 20 mg/ml TEV protease
Specific activity. ........................................................................................... ≥800 U/ml Reaction buffer (20x)
DNA content. ............................................................................................. ≤ 0.25 pg/U DTT solution (100 mM)
Exonucleases. ....................................................................................... none detected The individual components of this kit cannot be purchased separately.
Endonucleases. ..................................................................................... none detected 100 U correspond to 10 preparations with a volume of 100 µl.
3719.1 1 ml glass 29,90 € Type analysis:
Purity. .................................................................................................................≥90 %
3719.2 5 ml glass 115,00 € Concentration. .....................................................................................................1 U/µl
Functionality. .................................................................................................... passed
3750.1 100 U plastic 140,00 €
3750.2 1000 U plastic 940,00 €
For our extended assortment of Proteinase K, see chapter Biochemistry/Enzymes in our
current catalogue or at www.carlroth.com!
For ordering and information about prices and delivery in your country please contact us or your local distributor · info@carlroth.com 9
Molecular Biology
Enzymes for Cloning
Ligase T4 Ligase Tth
5 U/µl, for Molecular Biology 5 U/µl, for Molecular Biology
ATP-dependent recombinant ligase for molecular cloning. NAD-dependent ligase for molecular cloning.
Storage temp.: -20 °C • Transport temp.: cooled Storage temp.: -20 °C • Transport temp.: cooled
• Cloning of PCR and restriction fragments • Stable at high temperatures
• Self-circulation of linear DNA • Ligates double-stranded DNA
• Site-directed mutagenesis • Repairs single strand breaks in double stranded DNA
ATP-dependent recombinant ligase for molecular cloning, site-directed Tth DNA Ligase catalyzes the NAD-dependent formation of phosphodiester
mutagenesis, pitch repair in duplex DNA, RNA or DNA/RNA hybrids and bonds between adjacent 3'-hydroxyl and 5'-phosphate termini in double-
ligation-mediated PCR. stranded DNA. This ligase is inactive against single-stranded DNA or RNA
T4 DNA Ligase catalyses the formation of a phosphodiester bond between and blunting DNA.
adjacent 5'-phosphate and 3'-hydroxyl end groups in duplex DNA or RNA. The Tth DNA ligase is stable and active at much higher temperatures than
The kit consists of color coded tubes for easier identification to avoid conventional DNA ligases. The optimal ligation temperature range is 7-
confusion of reagents. 10 °C higher than that of the T4 DNA ligase and is determined by the Tm of
the substrates. High ligation temperature eliminates the nonspecific ligation.
The kit contains: The kit consists of color coded tubes for easier identification to avoid
T4 DNA Ligase confusion of reagents.
Ligation buffer (10x)
NEW Products and Programme Extensions
ATP solution (25 mM) The kit contains:
The individual components of this kit cannot be purchased separately. Quick Ligase
500 U correspond to 100 preparations with a volume of 20 µl. Ligation buffer (10x)
The individual components of this kit cannot be purchased separately.
Type analysis: 250 U correspond to 50 preparations with a volume of 25 µl.
Concentration. .................................................................................................... 5 U/µl
DNA nucleases....................................................................................... not detecable Type analysis:
Concentration. .....................................................................................................5 U/µl
3729.1 500 U plastic 89,90 € DNA nucleases....................................................................................... not detecable
3729.2 2500 U plastic 299,00 € 3736.1 250 U plastic 79,90 €
3736.2 2500 U plastic 499,90 €
Ligase quick RNase Inhibitor DNAse-/RNAse-free
5 U/µl, for Molecular Biology 20 U/µl, for Biochemistry and Molecular Biology
ATP-dependent DNA ligase for efficient ligation of sticky and or blunt ends. Inhibitor common enzymes such as RNase A, B and C.
Storage temp.: -20 °C • Transport temp.: cooled Storage temp.: -20 °C • Transport temp.: cooled
• Ligation in 5 to 15 minutes • Inhibits RNase A, B and C
• Cloning of PCR products and restriction fragments • For RNA isolation and purification
• Suitable for site-directed mutagenesis • Suitable for cDNA synthesis, RT-PCR and RT-qPCR
• Wide pH range
ATP-dependent recombinant ligase for molecular cloning, site-directed
mutagenesis, pitch repair in duplex DNA, RNA or DNA/RNA hybrids, The RNase Inhibitor is a 50 kDa recombinant human placental protein
ligation-mediated PCR. expressed in Escherichia coli. It inhibits ribonuclease (RNase) activity
Quick Ligase catalyses the formation of a phosphodiester bond between of common eukaryotic enzymes such as RNase A, RNase B, RNase C.
adjacent 5'-phosphate and 3'-hydroxyl end groups in duplex DNA or RNA.
The kit consists of color coded tubes for easier identification to avoid The RNase inhibitor is intended for use in applications where the presence
confusion of reagents. of RNases may pose a risk to RNA quality and experimental results, e.g.
RNA isolation, cDNA synthesis, RT-PCR, in vitro transcription and
The kit contains: translation or RNase-free monoclonal antibody production.
Quick Ligase The presence of 0,5-1 mM DTT is essential for optimal RNase inhibitor
Ligation buffer (2x) activity.
ATP solution (25 mM)
The individual components of this kit cannot be purchased separately. Guarantee analysis:
250 U correspond to 50 preparations with a volume of 20 µl. Purity. .................................................................................................................>90 %
Concentration. ...................................................................................................20 U/µl
Type analysis: RNase contamination. ...................................................................................... passed
Concentration. .................................................................................................... 5 U/µl Endonuclease contamination. .......................................................................... passed
DNA nucleases....................................................................................... not detecable Exonuclease contamination.............................................................................. passed
3733.1 250 U plastic 109,90 € 3727.1 200 U plastic 115,00 €
3733.2 750 U plastic 249,90 € 3727.2 10000 U plastic 440,00 €
10 Carl Roth GmbH + Co. KG · Schoemperlenstr. 3-5 · 76185 Karlsruhe · www.carlroth.com · Phone +49 721/5606 510 · Fax +49 721/5606 111Microbiology
Gelling Reagents
Agar
Agar-Agar, Noble quality
ultra pure
Ultrapure agar for immune diffusion and electrophoresis, culture media for
tissue cultures, bacteriology and mycology as well as tissue and cell culture.
Storage temp.: +20 °C
Due to an addtionional purification process the product obtained has an
excellent clarity, absence of color, excellent diffusion characteristics,
absence of toxic inhibitors, very low ash content and other impurities.
Guarantee analysis:
Particle size. ................................................................ >95 % through 60 mesh ASTM
Gel strength (1,5 % Nikan)...................................................................500-1200 g/cm2
Gelling temperature (1,5 % gel). .....................................................................34-38 °C
Melting temperature (1,5 % gel)......................................................................85-90 °C
Turbidity (1,5 % solution). ...............................................................................≤10 NTU
pH (1,5 % gel) before autoclaving......................................................................5,0-7,0
Electroendosmosis (EEO). ................................................................................≤0,450
NEW Products and Programme Extensions
Absorption (450 nm). .........................................................................................≤0,100
Resistance (1 % suspension). ................................................................. ≥20000 Ohm
3558.1 250 g plastic 139,00 €
3558.2 500 g plastic 239,00 €
Original pack 6 x 500 g 227,05/500 g
3558.3 1 kg plastic 419,00 €
Original pack 6 x 1 kg 398,05/1 kg
3558.4 2,5 kg plastic 819,00 €
Molecular Biological Nutrient Media
Granulated Media
Benefits of granulated Media: Terrific Broth, granulated
• Dust minimized for Molecular Biology
Nutricious medium for the culture and proliferation of E. coli.
• High solubility Storage temp.: +20 °C
• Without additives Granulated, dust-minimized version with high solubility and superior
homogeneity.
Terrific Broth is a nutritious medium for the culture and proliferation of E. coli.
Compared to LB medium Terrific Broth supports higher cellular density,
therefore increasing the yield during plasmid and protein preparations.
Application: Use 50,8 g for 1 l broth. Before autoclaving add 4 ml glycerol
per litre medium.
Formulation:
Casein, enzymatically digested............................................................................12 g/l
Yeast extract. .......................................................................................................24 g/l
K2HPO4...........................................................................................................12,54 g/l
KH2PO4.............................................................................................................2,31 g/l
pH value.......................................................................................................... 7,2 ±0,2
Granulation. ................................................................................................... complies
3556.1 500 g plastic 88,90 €
Original pack 6 x 500 g 84,46/500 g
3556.2 1 kg plastic 160,00 €
Original pack 6 x 1 kg 152,00/1 kg
3556.3 2,5 kg plastic 350,00 €
For ordering and information about prices and delivery in your country please contact us or your local distributor · info@carlroth.com 11Histology · Reagents
Roti®-Histol Nuclear fast red aluminium sulphate solution 0,1 %
for histology for microscopy
Clearing agent on base of limonene. For visualising nuclei e.g. during Elastica Staining.
For dewaxing of histological sections. Alternative product for xylene.
h Danger H318
n l j g Danger H304-H226-H400-H410-H315-H317
N069.1 500 ml glass 30,90 €
Roti®-Histol is a natural product, manufactured from untreated orange peel.
It consists of 96-98 % limonene, a terpene with a characteristic odour of
NEW Products and Programme Extensions
lemon or orange.
N069.2 1l New size glass 69,90 €
Roti®-Histol replaces current clearing agents without the need for changing Original pack 6 x 1 l 66,41/1 l
working procedures. No harmful vapours! It can be mixed with IPA, N069.3 2,5 l New size plastic 139,00 €
butanol, ethanol and all standard embedding media. Original pack 4 x 2,5 l 132,05/2,5 l
Clearing with Roti®-Histol prevents tissue samples from hardening and
brittleness. It is particularly suitable for manual work which usually exposes
the user to solvent vapours in high concentrations.
Roti®-Histol produces excellent results with Roti®-Histokitt mounting Paraformaldehyde
medium (Art. No. 6638) and special paraffins such as Roti®-Plast extra pure
(Art. No. 6642). Fixative
Please contact us for your free sample! Polyoxymethylene
(CH2O)n
6640.1 1l glass 27,90 € Storage temp.: +4-15 °C
Original pack 6 x 1 l
6640.4 2,5 l glass
26,51/1 l
56,50 €
l h n g Danger
H228-H302+H332-H315-H317-H318-H335-H341-H350
Original pack 4 x 2,5 l 53,68/2,5 l
Store product below 15 °C.
6640.2 10 l tinplate 160,90 €
Type analysis:
6640.5 5l aluminium 94,90 € Assay (acidim.). ...............................................................................................≥95,0 %
Original pack 2 x 5 l 90,16/5 l Ash content........................................................................................................≤0,1 %
Free acid (as HCOOH). .....................................................................................≤0,1 %
6640.6 25 l New size tinplate 379,50 €
0964.1 250 g plastic 11,90 €
Ethanol 96 %
0964.2 500 g plastic 14,90 €
Original pack 6 x 500 g 14,16/500 g
96 %, Ph.Eur., extra pure
0964.3 1 kg plastic 21,90 €
Ethyl alcohol, EtOH
Original pack 6 x 1 kg 20,81/1 kg
l g Danger H225-H319 0964.4 5 kg plastic 79,90 €
P075.1 1l glass 67,90 €
Original pack 6 x 1 l 64,51/1 l
P075.3 1l plastic 66,90 € To prepare a 4 % paraformaldhyde solution:
Original pack 6 x 1 l 63,56/1 l Mix 4 g of paraformaldehyde into 60 ml distilled water (milky liquid), heat with frequent
agitation to 60 °C. Add drop wise 1N NaOH, at pH 7 the solution becomes clear.
P075.2 2,5 l glass 142,90 € Add distilled water until the volume reaches 100 ml then let it cool down.
Original pack 4 x 2,5 l 135,76/2,5 l The solution is to be used immediately, because it is not stable at room temperature.
P075.4 2,5 l plastic 139,90 € Store in aliquots at -20 °C, is stable for approx. 6 months.
Original pack 4 x 2,5 l 132,91/2,5 l
P075.5 5l plastic 262,90 €
Original pack 2 x 5 l 249,76/5 l
P075.6 10 l tinplate 419,00 €
P075.7 10 l New size plastic 419,00 €
P075.8 25 l New size plastic 899,00 €
*Prices include German tax on alcohol
Contact international
Phone +49 721/5606 510 · Fax +49 721/5606 111 · info@carlroth.com · www.carlroth.com
Carl Roth GmbH + Co. KG · Schoemperlenstr. 3-5 · D-76185 Karlsruhe
Export prices might be higher.
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All Carl
andRoth GmbH
deliveries are+subject
Co. KG · Schoemperlenstr.
to the 3-5
terms and conditions of ·sale
76185 Karlsruhe
and delivery · www.carlroth.com
of Carl · Phone
Roth GmbH + Co. KG, +49 721/5606 510 · Fax +49 721/5606 111
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