MICORPS MONITORING MACROINVERTEBRATE
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WATERSHED Muskegon River Watershed Drains ~2,723 mi2 of land Starts at Houghton/Higgins Lakes down to its mouth at Muskegon Lake and, eventually, Lake Michigan
AQUATIC MACROINVERTEBRATES Small organisms that you can see without a microscope that lack an internal skeletal system and live part or all of their lives in water. Why Collect Macros? Good Indicators Good for Volunteers o Good indicators of stream conditions o Easy to sample (live there all the time) o Generally abundant communities o Macro diversity = Healthy stream o It is a unique experience o Threats to bug diversity o Sedimentation o Habitat loss o Chemical/organic pollution
NEW DATA SHEET Ordered from sensitive to tolerant 0 = Least tolerant; 10 = most tolerant Only 19 to know! Need at least 100 specimens < 30 – These are the worst of the worst samples. If you can't get 30 bugs in an hour's work, the stream is heavily degraded. Score a 10. < 60 – Certainly a problematic stream that should not be given a good score. Score a 7. 60-100 – No penalty, but always strive to get over 100 insects per site.
SAFETY GUIDELINES FOR STREAM WORK Keep your collector in Move slowly and sight cautiously Beware of instream items that could be scientific equipment or Wear life jackets if dangerous (like rebar appropriate and chunks of concrete)
COLLECTION PROCEDURE Collector gets in the water Wadable water 35-45 minutes of total effort 300-foot stream section Work upstream, always heading toward clean water (for visibility purposes) Sample all representative microhabitats: •Riffles • Stream Margins •Pools • Leaf packs •Cobbles • Undercut banks •Aquatic plants • Overhanging vegetation •Runs • Woody debris
COLLECTION PROCEDURES Shuffling, pulling, scraping, dragging, grabbing with hands The collector is not stopping to examine their net and never dumps debris back into the river...that's not their job! Collectors bring debris over to the team for immediate picking or put debris into a bucket for later picking Muck is miserable. Minimize muck mass.
PICKERS DUTIES Place macroinvertebrates into small jars of 70% ethanol or 70% isopropanol Strive for at least 60 creatures (>100 is preferable) They don't need to count them, just have a good general sense Keep everything found within the 60- minute picking time limit Aside: a head picker or Leader (with experience or trained), is a very helpful person to have on the stream bank.
DIRECT YOUR PICKERS TO... Don't prefer one type of creature over another Optional: If they pass >150 macroinvertebrates, you can have them stop Count and release any clam, mussel, snail, fish, and crayfish Exception: Provide New Zealand Mudsnail pictures. They should keep these snails and tell you about them if they saw them If you find a New Zealand Mudsnail, get in touch with an EGLE biologist
DECONTAMINATION New from 2021: Equipment decontamination with dilute bleach or other chemical like 409 is a required component of MiCorps sampling
IDENTIFICATION Don't do identification during picking except for light education purposes; the goal of this time is to pick An ID expert needs to confirm or do all identifications You can do ID after collection, or hold a different ev ent in the following weeks You should NOT... Do the official ID in the field Do the official ID on liv e creatures Hav e the v olunteers only sav e a few insects of each type Aside: sav e all samples for at least 5 years
SCORING- What is organic pollution? HILSENHOFF METHOD Pollution delivered to a stream through both point and non-point method • Strongly connected to oxygen Scores are secondarily Measures sensitivity to levels organic pollution (0-10) connected to habitat quality and flow • From natural, agricultural, and urban sources • Waste-water Areas with higher organic pollution will have More bank erosion; fine degraded habitat through sediment; flashy water • Fertilizers and nutrients landuse/development flows impacts • Pesticides
WATER QUALITY RATING (WQR) 1. Add Total Abundance 2. Multiply: Count x Sensitivity for each line 3. Sum the Count x Sensitivity Column 4. Divide that Sum (Count x Sensitivity) by the Total Abundance
VARIATION IN PROCEDURE AND LOGISTICS Total collection effort should be 35- 45 minutes no matter the number of collectors Collectors must be trained ahead of time w/ practice sampling session 300-foot stream sections Follow MiCorps collection procedures: Don't pick for more than an hour (can be less if full time isn't needed) Don't do your official ID's out in the field. Kill the specimens in alcohol, ID in a controlled setting with an expert. Keep the specimens. All macros must be collected in a 2-week window that is similar year after year
HABITAT ASSESSMENT Helps us understand WHY we are finding theses macroinvertebrates or why their populations are changing It is a qualitative assessment, not quantitative Your team will fill out a form answering questions on Stream Size • Plant community Flow • Size/condition of riparian Presence of foam/oil/trash zone • Sources of degradation Stream bank condition • Optional pebble count **We will walk through assessment during the field sampling day
HABITAT ASSESSMENT How often? Once every 5 years When to hold it? After collecting macroinvertebrates Summer is a good time to conduct a habitat assessment (vegetation types and density are part of the assessment)
HABITAT ASSESSMENT How to hold the assessment 1. The group needs to spend some time at the stream before answering the habitat questions. Explore and poke around the entire 300-foot reach 2. One person walks the group through the data sheet 3. The group, by consensus, rates different aspects of the habitat 4. If the group can't agree on the answer, you take averages (when numbers) or record comments
MACROINVERTEBRATE IDENTIFICATION
KEY BODY PARTS
ORDER – MEGALOPTERA: TWO FAMILIES Sensitive Hellgrammite-Dobsonfly/Fishfly Alderfly Sensitive 0→ • Family-Corydalidae • Family-Sialidae 1 1 2 2 3 Characteristics Characteristics 3 4.0 → 4 • Lateral projections • Lateral projections 4 5 • Three pairs of legs • Three pairs of legs 5 6 6 • No distinct tail; prolegs • Distinct single tail 7 7 8 • Generally larger • Generally smaller 8 9 • Often found on wood • Often found in silt 9 10 10 Tolerant Tolerant Family-Sialidae
Sensitive 1.3 → 1 ORDER-PLECOPTERA: STONEFLIES 2 3 Characteristics 4 • Two tails 5 • Three pairs of legs 6 • Two tarsal claws • Long antennae 7 • Sometimes gills are on underside of thorax 8 but never running up and down the top of the abdomen 9 • Often found in gravel or on cobble and 10 wood Tolerant
Sensitive 1 ORDER-TRICHOPTERA: CADDISFLIES 2 3.2 → 3 Characteristics • Three pairs of legs 4 • Wormlike 5 • Often with plates on thorax • Prolegs on end of abdomen with claw 6 • Often in a case (sand, leaves, twigs, etc.) 7 • Some types are free-living 8 • Found in many habitats 9 10 Tolerant
Sensitive ORDER – EPHEMEROPTERA: MAYFLIES 1 2 Characteristics 3.5 → 3 • Three tails 4 • Long antennae • Three pairs of legs 5 • Feathery or plate like gills on abdomen 6 NOT thorax • Found in many habitat types 7 8 9 10 Tolerant
Sensitive ORDER-ODONATA; SUB-ORDER: 1 ANISOPTERA (DRAGONFLY) 2 3 4.0 → 4 Characteristics 5 • Stout body 6 • Labium "mask" to catch prey 7 • Two pairs of wing pads • Large eyes 8 • Three pairs of legs 9 • Five stiff points on end of abdomen 10 Tolerant
Sensitive ORDER-ODONATA; SUB-ORDER: 1 ZYGOPTERA (DAMSELFLY) 2 3 4 5 Characteristics • Slender body 6 • Two pairs of wingpads 7.7 → 7 • Three pairs of legs 8 • Labial mask • Three tails (gills) 9 10 Tolerant
THREE TAIL CONFUSION... Damselfly Mayfly Does not have gills along abdomen Has gills along abdomen
Sensitive ORDER-COLEOPTERA: 1 BEETLES 2 Characteristics 3 • Generally harder bodies (Elytra) 4 • Oval shaped 5.1 → 5 • Chewing mouthparts 6 • Well-developed eyes • Three pairs of legs 7 • Found in many habitats, but many like slower 8 flowing water and vegetation 9 • Both adults and larvae live in water and look • very different 10 Tolerant
BEETLE LARVAE Characteristics • Eye spots and antennae usually visible • Mandibles often visible • Three pairs of segmented legs (4-5 segments of each) • Sometimes lateral appendages at end of abdomen
CONFUSION EXPECTED!! BEETLES, ALDERFLIES, AND DOBSON FLIES 1. Beetle (Hydrophilidae) 2. Beetle (Dytiscidae) 3. Alderfly (Sialidae) 4. Dobson Fly (Corydalidae) 5. Beetle (Gyrinidae)
BEETLE ,ALDERFLY OR DOBSONFLY LARVAE? Alderfly Beetle Beetle Hellgrammite- Dobsonfly Beetle
CADDISFLY BEETLE LARVAE LARVAE
ORDER-DIPTERA "TRUE FLY" Larvae General Characteristics • So many kinds!!! • Very small • Soft bodied/worm like • No legs • No visible wing pads • Many have poorly developed head • Some have fleshy or filamentous things that may look like a tail
MICORPS SIMPLIFIED TAXA LIST FOR FLIES Listed by sensitivity 0 = least tolerant 10 = most tolerant
Sensitive ORDER-DIPTERA: THREE FAMILIES OF 1.0→ 1 TRUE FLIES (SENSITIVE) 2 Water Snipe Fly (Athericidae) 3 Characteristics 4 • Found in fast flowing water • Burrow in gravel 5 Net-Winged Midge (Blephariceridae) 6 Characteristics Dixid Midge (Dixidae) Characteristics • Suction cups on each body division 7 • Flat terminal lobes fringed with hair • Prolegs with hooks on abdominal 8 sections 1-2 9 • Can see head • Found in shallow/slow water with 10 plants Tolerant • Found on surface of water when picking
Sensitive ORDER-DIPTERA: TRUE FLIES 1 TOLERANT TO POLLUTION 2 Mosquito (Culicidae) Characteristics 3 • Head capsule visible • Fused thoracic segments (bulb shape) 4 • Found in slow water 5 • Breathe air with spiracle Soldier Fly (Stratiomyidae) Characteristics 6 • Flattened body 7 • Leathery looking • Can see head 8 8.7→ Rat-tailed Maggot/Hover Fly (Syphridae) • Found in muck Characteristics 9 • Pollution tolerant • Cannot see head 10 • Mouth hooks Tolerant • Long spiracle to breathe air • Pollution tolerant
DIXID MIDGE MOSQUITO Sensitivity = 1 Sensitivity = 8.6
ORDER-DIPTERA: TRUE FLIES NOT LISTED AS Sensitive SENSITIVE (1) OR TOLERANT (8.7) SOMEWHAT SENSITIVE 1 2 Black Fly (Simulidae) Characteristics 3 • Bowling pin shape 4 • Stand up in water column • Filter fans at head 5 • Found attached to vegetation, rocks, logs 6.0 → 6 Midges (Chironomidae) Characteristics 7 • Head exposed • Thoracic and anal prolegs 8 9 Cranefly (Tipulidae) Characteristics • Found in leaf packs 10 • Cannot see head Tolerant • Abdomen end varies • Can get large
Sensitive 1 ORDER-HEMIPTERA: TRUE BUGS 2 3 4 Characteristics 5 • Piercing/sucking mouthparts • Wings hardened near the base and membranous 6 everywhere else • Three pairs of legs 7.7 → 7 • Adults look same as young 8 • Found in many habitats, but many like slower flowing water and vegetation 9 10 Tolerant
Sensitive ORDER-AMPHIPODA: SCUD 1 (FRESHWATER SHRIMP, SIDESWIMMERS) 2 3 4.0 → 4 5 6 Characteristics 7 • Two pairs of antennae 8 • Seven pairs of legs • Flattened (laterally) 9 • Often found in silt or along stream banks 10 Tolerant
Sensitive 1 ORDER-ISOPODA: SOWBUGS 2 3 Characteristics 4 • Two pairs of antennae 5 • Seven pairs of legs 6 • Flattened (dorsally-ventrally) 7 • Leg-like gills on posterior end 8.0 → 8 • Adults look same as young 9 10 Tolerant
Sensitive 1 ORDER-DECAPODA: CRAYFISH 2 3 Don't collect, but count them 4 5 6.0 → 6 7 8 9 10 Tolerant
Sensitive ORDER-VARIOUS CLAMS AND 1 FRESHWATER MUSSELS (BI-VALVES) 2 3 4 Don't collect, but count them 5 Characteristics • Very old, slow growth 6.9 → 6 • Many are special concern 7 • Place carefully back into sediment 8 the way you found them • Don't count empty shells 9 • Some are tiny 10 Tolerant
Sensitive 1 ORDER-VARIOUS SNAILS 2 3 4 Characteristics • Many types 5 • Watch for New Zealand Mudsnail* 6.9 → 6 • Don't count empty shells 7 Don't collect, but count them 8 Collect zebra mussels 9 *If you suspect a mudsnail at a location that is 10 not known to be infested, please contact Paul Steen or EGLE Tolerant
Sensitive 1 ORDER-VARIOUS: LEECHES 2 3 Characteristics 4 • Many, many striations (surficial only) 5 • Two suckers: front and back 6 7 8 9 10 → 10 Tolerant
Sensitive 1 ORDER-VARIOUS: AQUATIC WORM 2 3 4 5 6 Characteristics 7 8 • Segmented 9 • May look like an earth worm 10 → 10 Tolerant
QUESTIONS?
NEXT TRAINING SESSION Marshall Memorial Park, Newaygo: 246 State Rd, Newaygo, MI 49337 10am-1pm Meet in parking lot Need to bring: water, sunscreen, bug spray, waders (we can supply), good walking/hiking shoes (there will be walking) Official Sampling: May 20th 11am-2pm @ Ed Henning Park, Newaygo (Wheeler Drain and Brooks Creek) SarahKrzemien@ferris.edu
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