AlpBioEco Results and Replicable Roadmap - Analysis of the bio-based value-chains apples, walnuts and herbs
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AlpBioEco Results and Replicable Roadmap Analysis of the bio-based value-chains apples, walnuts and herbs www.alpine-space.eu /alpbioeco
2 3 Contents Bioeconomy CONTENT BIOECONOMY Bioeconomy.......................................................................................................................... 3 Bioeconomy means using natural substances dependency on fossil fuels and improves sustain- for new and alternative products. The most ability. New business models and cross-industrial AlpBioEco project..............................................................................................................4 important goals are to replace fossil resources innovations are the basis for such transitions. with renewable materials and to find applications With its raw material sources and diverse eco- 1. APPLES .................................................................................................................................... 5 for by-products and waste materials. Synergies nomy, the Alpine region has good starting of technology, ecology and sustainability are conditions. At this point, the AlpBioEco project 1.1. Market analysis.................................................................................................................... 5 harnessed to create new opportunities and comes into place to foster development towards 1.2. Laboratory analysis ..........................................................................................................6 prospects for economic activities. By targeting bioeconomy by investigating the valorisation an economic cycle, the concept of bioeconomy of innovative potentials along bio-based food 1.3. SWOT apples .....................................................................................................................10 comprises raw material sources of agriculture, and botanical extract value chains. In the first 1.4. Conclusion............................................................................................................................10 forestry, water management and waste (food work package, AlpBioEco examines the bio- and feed, materials, energy). The transition from based potential of the three value chains apples, a fossil to a bio-based economy reduces society‘s walnuts and herbs. 2. WALNUTS............................................................................................................................... 11 2.1. Market analysis................................................................................................................... 11 2.2. Laboratory analysis.........................................................................................................14 2.3. SWOT walnuts....................................................................................................................16 2.4. Conclusion............................................................................................................................16 3. HERBS..................................................................................................................................... 17 3.1. Market analysis herbs in general.......................................................................... 17 3.2. Laboratory analysis ........................................................................................................19 3.3. SWOT herbs ....................................................................................................................... 23 3.4. Alpine hay............................................................................................................................24 3.4.1 Market analysis.................................................................................................................24 3.4.2. SWOT Alpine hay............................................................................................................ 25 APPLES 3.5 Conclusion........................................................................................................................... 25 4. Replicable roadmap & bottom line.................................................................... 26 WALNUTS 5. Bibliography.......................................................................................................................28 HERBS Photo: apple © planet_w lee n kim /// Photo: Walnut picking season walnut tree branches of walnuts opened the shell and the collected walnuts are kept in bulk and broken walnut which is composition on the table Shell nut © allamimages /// Photo: close view on fresh herbs bunch © marcin jucha AlpBioEco Results and Replicable Roadmap AlpBioEco Results and Replicable Roadmap
4 5 AlpBioEco project 1. Apples & 1.1. Market analysis ALPBIOECO PROJECT 1. APPLES AlpBioEco was launched in 2018 and investigates with 13 project partners in the Alpine region new bioeconomic potentials. It will contribute to the framework conditions for innovations that Apples are mostly associated with table fruits or juice. It appears that the apple market will lead to eco-innovative business ideas and concepts for SMEs. The aim is to promote and is a saturated one economically. This chapter examines the individual levels of the value implement eco-innovation in practice while applying a cross-sector, multi-level stakeholder chain (production, processing and marketing) such as the associated and researched approach. In the first phase, market and laboratory analyses for the exemplary value chains of bioeconomic potentials of apples and its by-products (laboratory research). The chapter apples, walnuts and herbs including Alpine hay are conducted to gain a better understanding outlines the economic and market dimensions as well as the capabilities of the sectors. and to develop new products. In the next phase, feasible business models for these ideas are created for strengthening local and regional value chains. These concepts will be tested in the third phase before recommendations can be made in phase four. For the geographical delimitation of the study area, the Alpine Space was defined according to the EU Interreg Alpine Space through which AlpBioEco is co-financed (www.alpine-space.eu). 1.1. MARKET ANALYSIS First of all, desk research surveyed secondary potential, which was rated as ‘very good’ especially The Alpine Space is characterised by a great diversity of raw materials, local products and statistical sources, which include international for organically cultivated table apples by the industries with great bioeconomic potentials. In order to create future perspectives for the local databases (e.g., FAOstat, Eurostat) and which experts in the online survey. Besides drinks like actors of the Alpine Space and to point out new business opportunities, AlpBioEco focusses were integrated with regional analyses of the juice with high market potential, non-food pro- on the common value chains of apples, walnuts and herbs including Alpine hay. In the first respective state institutions from the single ducts like animal feed, fertilisers (based on po- work package the potentials of the three value chain products were focused by a two-stepped countries. Furthermore, available results of mace) and apple wood were assessed with low process – market and laboratory analyses. For guiding the research and ensure transferability, publications and statistics on agricultural eco- to mean market potentials. All other non-food criteria were developed based on the project partners experience (see Table 1). nomics, marketing and fruit cultivation were products were rated as having a low or medium systematically evaluated in collaboration with market potential in the online survey. Value chain steps, criteria and FAQs Information access the AlpBioEco partners. Secondly, the gathered data were validated and supplemented through Regarding the market potential of processed general: What seems to be the bioeconomic potential? > lab analysis quantitative and qualitative expert interviews. goods, according to the survey a high market > market analysis potential was estimated for the organic com- raw material: Which ingredients are purchasable or easy to access? > literature review The apple cultivation in the Alps is concentrated modities. By-products of the juice production Which ingredients are already used for what? > market analysis on some primary regions. The largest contigu- like pomace show high bioeconomic potentials. > online research ous cultivation territory in terms of area is in the Other innovative products like apple leather are processing: Which by-products or leftovers in the value chain can be identified? > expert interviews region of Trentino Alto Adige, which is also the currently expensive niche products. Through > lab analysis most intensively cultivated one (see Figure 1). In innovative procedures (Gramm et al., 2019), the contrast to other regions small farms are very im- by-products could be used for medical purposes processing: What are product and process innovations? > literature review portant (Garming et al., 2013) and well organised (pectin, food supplements), cosmetics (apple > online research within a cooperative system, while for instance essence, seed oil), cleaning agents and other economic/comparative advantage: Can local products substitute imports? > experts-questionnaires/ in Baden-Württemberg a trend towards larger products (apple leather, paper). All involved fields literature review farms was noticed. In general, the research has such as production, processing and marketing shown that apples are currently mainly proces- are the basis for the development of novel inno- enterprises: What are the involved companies? > expert interviews > market analysis sed into products in the food sector for eating vations and further scientific analyses. It appears > online research apple (Eurostat, 2018), juice, vinegar, puree, pec- that apple, regarding this matter, is a mature tin, chips. Eating apples obtain prices up to three saturated market. environmental: Which type of energy is consumed in the VC, at what level? > stakeholder involvement times higher than other products. Therefore, external – public funding and interest, subsidies: Are there ways to finance > expert interviews achieving the highest possible proportion of ta- innovative approaches (coming up)? Are there institutes of the education > online research ble apples has a great priority for the producers. sector which can cooperate (with low financial risks)? The monetary value is reflected in the market risks – environmental/economic/social/legal/regulatory: What are strengths or > SWOT weaknesses? What are accelerators and brakes? social: Is there a risk of the VC causing conflicts/tensions in society? > expert interviews > literature review > online research/social media Table 1: Criteria for the two ways of analysis SWOT analyses for all value chains were conducted to evaluate internal and external influencing factors. Internal factors are divided into strengths (S) and weaknesses (W) looking at processes, structures and preconditions. External factors are opportunities (O) and threats (T) occurring through the market and the surrounding environment (see chapter 1.3, 2.3, 3.3, 3.4.2). Subsequently, a repli- cable roadmap as a graphical representation of the methodological approach for application in other bioeconomic value chains was created. The following chapters describe these value chains based on the conducted market and laboratory analyses followed by the SWOTs. AlpBioEco Results and Replicable Roadmap AlpBioEco Results and Replicable Roadmap
6 7 1.2. Laboratory analysis 1.2. Laboratory analysis This chapter describes fundamental results regarding raw material characterisation, feasibility analysis for apple wax extraction, phenol extraction as a food additive, yogurt fermentation with apple pomace for a dietary fibre enrichment, smoothies and 3D-printed snacks (figure 2). Additional discovered potential ideas, which have not been analyzed in the laboratory are listed in the following table 2. OH WAX OH Non-food: bio-degradable wax, wraps, HO O ski wax, floor wax (extracted from pomace) POMACE OH Food: aroma compounds, OH O flavoring, dietary supplement, POLYPHENOL EXTRACTS oil, 3D printed snacks Non-food: pellets, Food: supplement, yogurt supplement, high-protein fermentation feedstock, high-fiber Entails bioactive substances, liquid or powder form (extracted from pomace) Figure 1: Most important apple-growing regions (NUTS 3) in the Alpine Space. Source: Gramm et al., 2019 The existing bioeconomic potential of apples and its by-products are rarely used in the APPLE Alpine Space. Many applications are still under development and lack in economic efficiency. The research indicates that in particular the apple pomace has great potential and could be utilised for various applications, for example with compression moulding for cutlery and Figure 2: Applications for apple pomace /// Photo: apple pomace © hjochen /// Photo: Fresh red apple isolated on white. © irin-k straws. Innovative products are currently niche products with high prices in the Alpine Space. New applications were analysed in the laboratory analysis, which is described in the following. The total colony count, particle size and dry tration was determined in dry apple pomace matter are determined as a basic raw material with 8,77*102 CFU/g. The difference in bacteria characterisation for further product specific concentration between humid and dry apple 1.2. LABORATORY ANALYSIS experiments. All apple pomace samples of the pomace is logically comprehensible. The The basic research is an important skill to provide a technical overview of already investigated microbiological analysis have been tested bacterial growing was minimised due to the fields regarding the reuse of apple waste material and by-products. Also, it is a basic structure on PCA for a total bacterial count with an drying process. The diversity of bacterial conta- for discovering potential and novel innovations. That is the reason, why basic research was done incubation period of 72 h at 30°C. The highest mination in various humid apple pomaces is by a mind map method. By pre-defined target material and focus areas as cleaning agents and bacteria concentrations with 6,27*104 CFU/g explainable with different processing steps, cosmetics, food additives, high tech methods, and renewable products, 13 ideas were selected. and 1,53*103 CFU/g were found in two different storage conditions and raw material properties. Several ideas, which are not yet on the market, were chosen to investigate them on a laboratory apple pomace batches. The lowest concen- scale. The value and necessity of laboratory activities are to verify available functionalities. All required methods and references can be found on our homepage. AlpBioEco Results and Replicable Roadmap AlpBioEco Results and Replicable Roadmap
8 9 1.2. Laboratory analysis 1.2. Laboratory analysis For a wax extraction from apple pomace the Scope Potential References standard Soxhlet extraction method was used. Also, as pre-treatment, acid digestion was done apple wax + alternative of ski waxes organofluorine compounds possible ski wax: Nordic following Matissek et al. 2014 for improving the changing regulations regarding ski wax compositions Ecolabeling 2018 ; wax structure and increasing the yield. This study + different labelling companies chose to set requirements for Rogowski et al. 2005 and research of Günther Lang et al. 1996 have other constitute substances Floor treating: Smith and Murphy 1986 obtained comparable results in testing diffe- + possible maintenance & polishing ability rent apple pomace extraction methods in the + natural & degradable wax cosmetic sector. According to our performed + wax amount of various floor treating & cleaning agents experiments, a higher yield is reachable with in- replaced good odour dustrial dried and depectinized pomace. Equally, subsequent treatment of the wax extract with a wood like + alternative energy source Verma et al. 2012 vacuum evaporator had a positive effect on pellets + agro pellets for domestic heating boilers the yield. Wax wraps are promising and an ideal + highest combustion efficiency (91,3%) after wood pellets (92,4%) alternative to plastic wraps to preserve food. Usually, they are made from expensive bee’s leather/ + natural imitation leather environment risks Alberto Volcan 2009 wax, which can be replaced with extracted wax paper + bio-degradable from apple pomace. Figure 3 shows an exem- + advantages: not inflammable & not toxic energy source plarily apple wax wrap model. The layer of the wrap from apple wax was softer and more agile disposable + bio-degradable plastic replacement: sustainable alternative with Gautam and Caetano 2017; in comparison to bee wax. From this, it can be cutlery & minimal impact when obtained in sustainable manner Razza et al. 2009 concluded that apple wax can be additional straws + existing cutlery from areca palm & coconut tree www.wisefood.de raw material for making wax wraps and an + interesting for fast food restaurants, canteens & festivals Figure 3: Exemplarily apple wax wrap model Photo: Apple wax © MCI alternative of plastic. removal of + removal potential of different dyes used in various industry sectors Robinson et al. 2002 dyes by (textile, food, plastics, paper) biosorption + alternative of expensive active carbon A polyphenol extract from apple pomace can be used as a food additive in various products. + for the removal of dyes from a synthetic effluent 1 g of pomace The result of first polyphenol extraction experiments shows a low yield of 1,38 g Gallic acid (particle size 600 µm) equivalent/kg pomace. The efficiency and realisation of profit have to be assessed individually depending on the effort and feasibility of each eligible company. cat litter + alternative for bentonite cat litter www.jelu-werk.com + existing alternatives: litter from wood (absorbs moisture quickly, www.yarrah.com binds odours, ecologically, compostable), clay & peanut hulls Stephen L. Ivie 1992; The apple pomace in the yogurt fermentation has a potential for a dietary fibre enrichment and Raymond J. Fry 1974 can influence the texture positively. The result of the germ differentiation by MALDI-TOF shows us + high absorption potential of apple pomace the necessity of suitable hygiene measures and after treatments directly by the manufacturer. dietary fibre + non-caloric bulking agents for partial replacement of flour Galanakis 2018; enrichment (carbohydrates), fat & sugar Struck et al. 2018; Printed smoothie snacks (figure 4) are a possibility to increase the consumption of healthy food. + apple dietary fibre granules Quiles et al. 2018; Regarding to first impressions, the printing quality can be improved with the added apple pomace, Rodríguez Madrera especially the viscosity and firmness. + increased content of phenolic compounds after fermentation et al. 2015 of dried pomace in bread production Table 2: Results of additional discovered potential ideas with apple pomace A meaningful outcome of all performed experiments in the laboratory is the finding that high efficiency can only be reached with the usage of the entire by-product. Preceding processes like extraction can decrease efficiency through further costs and time exposure. Figure 4: Printed cube from apple pomace /// Photo: Printed cube from apple pomace © MCI AlpBioEco Results and Replicable Roadmap AlpBioEco Results and Replicable Roadmap
10 11 1.3. SWOT apples & 1.4. Conclusion 2. Walnuts & 2.1. Market analysis 1.3. SWOT APPLES 2. WALNUTS Internal factors External factors Walnut trees are well known for their largeness, beauty, good workable wood and tasty edible nuts. But there is more to discover: this chapter focuses on cultivation, processing, Strengths Weaknesses Opportunities Threats refining and marketing aspects of walnuts in the Alpine Space as well as potentials of barely used raw materials and by-products coming out of the laboratory analysis. • huge network of actors • organic apple production • increase in exports • competitive pressure due • many different sorts is more cost-intensive than • product quality, variety to increased production conventional diversification for market outside Europe • already established quality marks • pests definition • economic uncertainties • Italy: one of EU‘s most im- • high production costs • apple industry waste in residual inventories 2.1. MARKET ANALYSIS portant apple producers • unattractive appearance (kernels, shells) to produce • global warming vs. (33% export share) of the growing areas sustainable materials variety suitability In order to get an overview of the market for the value chain of walnuts, several information sources • production chain simple, (tourism) • waste from the apple • high costs for external were used. Extensive desktop research was used to gain the available knowledge from databases and well organised • lack of processing, industry for production of equipment publications. Secondly, the relevant information was prepared for an e-mail survey on sub-regional technology structures, phenolic antioxidants • high dependency characteristics in the Alpine Space. These tailor-made questionnaires were then presented to experts • organic cultivation increa- singly important lack of innovative strength • transnational networked (diseases, crop failures) from all targeted countries, who were identified in close cooperation with the project consortium. in marketing structures research • tourism (landscape, • many years of experience The response rate was low but the quality of the expert knowledge and the intensity of the exchange in fruit growing • classic production • experience in mountain conflicts of interest) competes with agriculture (machinery) guarantee a solid information base. Figure 5 illustrates the different steps taken in this approach. • specialised research & • shortage of land bioeconomic use • production units adapted (housing, prices) educational institutions with focus on apples • subsidies are weak to climatic zones • subsidy reduction • orchards are under • large amount of STEP 1 STEP 2 STEP 3 POTENTIALS • different climate zones • water (quality, quantity) Draft Qualified with good conditions for pressure (near to outer by-products Desktop Expert Reporting edge of settlements) • production costs vs. market market different varieties • high bioeconomic market prices Research involve- potential (tourism, energy profile profile • successful regional (international comparison) ment projects sector, quality products with image) • price fluctuations • extensively used orchards • lack of acceptance for use promote biodiversity • high market potential for of pesticides in society + Data research + Email survey + (Carto)graphic processing companies, + Literature review among experts output • regional traditions technology providers • regional construction (recipes) boom in rural areas + Expert interviews + Textual interpretation • regionality • important for characteris- tic in the regions Figure 5: Method of market analysis Table 3: SWOT Apples In food applications, kernels are used (complementary or oil) for fresh consumption, dry fruit/snack (e.g. nut mixes), as valuable ingredients in butter, pesto, cheese, salads, cakes (e.g. Engadiner Nusstorte, 1.4. CONCLUSION Baklava, Grison’s cake), bread (Bavarian fruit bread) and meat. Unripe green walnuts are used for local traditional dishes like black nuts, liqueur (Nocino) and pickled green walnuts. The comprehensive analyses of apples indicate into opportunities by applying the listed useful bioeconomic potentials. The outcome of strengths coupled with subsidies and extension In non-food applications shells can be used as cat litter, building material and thermoplastic, dried this research is that the ideal target material for of the corresponding opportunities. Research leaves for feed, paper and tea, oil for skin care/soap because of its higher contents of polyunsaturated an innovation process is apple pomace because and the corresponding expertise in cultivation, fatty acids which may give walnuts additional anti-atherogenic and cosmetic value (Milind & Deepa 2011). it is available in large quantity as a waste product processing and marketing will serve as the The green shells are utilised for colouring (e.g. hair or textile dye) and as a blasting agent. The walnut in the juice industry. The technical screening basis for exploiting and implementing the wood is used for furniture, jewellery and decoration. Besides these applications walnut products are depends on which kind of reuses and upcycling opportunities and counter threats along the established as folk medicine in naturopathy and as house remedy (use of walnut oil, kernels, walnut possibilities already exists with apple pomace value-added chain. leaves, walnut seeds, etc.). Other usages are grated walnut shell as a cleaning and polishing agent and especially where still potentials are. Howe- (e.g. for metals, fiberglass), walnut granulate for cleaning intake systems in diesel engines, textile ver, all performed experiments in the laboratory Further information can be found dyes (use of the green peel), animal feed, briquettes/lighters, insulating material or organic pesticide show that high efficiency can only be reached in the download section on the (use of walnut tree leaves). The cultivation areas in the Alpine Space are visualized in Figure 6. The with the usage of the entire by-product. Prece- AlpBioEco-homepage. opportunities for bioeconomic exploitation of walnuts and their by-products are shown in table 4. ding processes like extraction can decrease effi- ciency through further costs and time exposure. It will be possible to turn the listed weaknesses AlpBioEco Results and Replicable Roadmap AlpBioEco Results and Replicable Roadmap
12 13 2.1. Market analysis 2.1. Market analysis Figure 6: Cultivation areas of walnuts localized (experts‘ assessments) GREEN SHELL HARD SHELL Walnut shell ointment, Cosmetics, cat littler, tincture colorants in building material peeling, cosmetics, textiles, tanning granules as blasting agent KERNEL GREEN WALNUTS Food; Cosmetics; Food: pickled green Press cake from oil pressing: walnuts, liqueur, syrup Food, feed, cosmetics LEAVES WOOD, ROOT, BARK Food: tea; other: fish narcotics; Furniture, carvings, … macerate, volatile oil, tincture, bug spray, fertiliser, paper WALNUT TREE (Juglans regia) Figure 7: Known applications of walnuts /// Photo: Branch of walnut isolated on white background © Anton-Burakov /// Photo: Green walnuts with leaves isolated on a white background © Valentyn Volkov /// Photo: Green walnuts on a white background © TADDEUS /// Photo: Empty walnut shell isolated on white background © NattikaPhoto: Kernel walnut isolated on the white background closeup © Urfin /// Photo: Dry branches isolated on white background © Chengyu /// Photo: Old walnut tree on a white background © Zerbor Bioeconomic exploitation opportunities Limits + organic pesticides of leaves - know-how and knowledge The walnut tree is a very valuable plant which offers a great variety of raw material for manifold uti- + shell for biofuel, briquettes, lighters, animal feed or - marketing strategies to support the sale lizations (seen in figure 7). The market segments nutrition, wood processing, cosmetics, medicines insulating material of bioeconomic products and also other usages of walnuts and their by-products (e.g. tree leaves, green peel, walnut shell) pre- + hull for textile (Milind & Deepa 2011: 14) - complete exploitation of the nuts and their sent the processing possibilities for walnuts. In summary, the market of walnuts in the Alpine Space + grated walnut shell (walnut flour) as cleaning and by-products by the producers can be described as a small-scale market with mainly small producers and a few major processing polishing agent (Milind & Deepa 2011: 13) - interest of producers and processors: economically inefficiency (costs for machines) companies. The market is characterised by an investment backlog, especially regarding investments + aromatic essentials from green nuts - information by the growers of walnuts about in processing infrastructure. Positive general market developments can be recognised during the the quantities of by-products possible for last five years (rising sales prices, supportive consumer trends, regionalisation in the value chain). processing companies However, these positive market developments have not yet fully reached the local level. Moreover, regional walnut farmers within the Alpine Space are in serious competition with large and cheaper Innovation potentials Obstacles to innovation imports of international walnut producers (from China, Iran, Latin America, USA). Securing or rea- ching a competitive position means in particular to ensure quality. + process innovation: expansion of regional - limited processing capacities (cracking machines, processing capacities and service offers for walnut harvesters) and structures (great distance to the producers (cracking machines, oil mills) nearest processing service) Amongst the multiple experts‘ proposals, many ideas and recommendations for + innovation/regionalisation in the value chain: sale - partially insufficient quality of the walnuts action seem promising. The following four types of activities are potentially efficient: of walnut kernels to regional customers (health (too small, moist) food shops, processors of refined walnut products) - small harvests due to small-scale cultivation cannot + establishment of joint quality assurance guidelines by producers and processors + product innovation: bioeconomic exploitation/ meet the demand for raw materials complete processing of kernel, membrane, shells +s upported knowledge exchange between producers and processors (quantities, processing - decentralised cultivation structures limit the processing quantities of the walnut possibilities of certain species, bioeconomic potentials) (100 % processing is currently unrealistic) + exploring alternative processing possibilities - high competition with cheap imports +n o one-size-fits-all solution, but activities should be established concerning the local cultivation and processing situation (establishment of cooperatives, funding of regional Table 4: Bioeconomic exploitation opportunities of walnuts and their waste residues (experts’ assessments) mills, cracking machines) AlpBioEco Results and Replicable Roadmap AlpBioEco Results and Replicable Roadmap
14 15 2.2. Laboratory analysis 2.2. Laboratory analysis 2.2. LABORATORY ANALYSIS Besides uses in the food industry, new ideas for the utilisation of walnut components in cosmetics were developed such as peeling cream with grinded walnut shells, after shave balm with oil & extract The literature shows knowledge gaps concerning randomly selected press cake flours and one as a and shower oil including feasible conservation tests. Other applications are the use of leaves in ingredients of walnuts (Parle/Khanna, 2011; Schle- bar. All press cakes show differences in colour from tea or tincture and paper production. Paper characteristics regarding brittleness, color and grain gel, 2016; Böllersen, 2017). For this reason, sensory light to dark brown and in their texture which depend mainly on composition and leave proportion (figure 10). Furthermore, the investigation of and chemical analyses for some components supports the assumption of inconsistent materials. hard and green shells showed colouring properties that can be used for cosmetic and for textiles. were conducted. Various application tests followed For high-quality levels, the material should be First tests for colouring natural fibres (cotton, regional fabrics from nettle, hemp fibres) demonstrate this review. tested beforehand or purchased from the same a great colour spectrum depending on the pre-treatments. tree and mill. Firstly, a sensory evaluation with a trained panel The first results showed a wide range of possibilities and ideas with all parts of the walnuts. Its be- of regional walnut kernels was conducted. The indi- Thirdly, for finding bioeconomic potentials, known nefits can be used in the food, cosmetics, textile and paper industry and various other areas. Many vidual batches indicated no significant differences: applications with walnuts for food, cosmetics and applications of walnut kernels (oil, press cake), green nuts and walnut leaves were tested in our pilot probably kernels from one tree have more varieties others were searched. Based on this, new ideas plants in the kitchen lab. All products showed the typical taste of walnuts. Therefore, it is advisable to than the ones from other trees. These results sug- were generated by using creative combine the walnut press cake with other starchy products to minimise the taste (mostly bitter, as- gest that there is no consistent raw material which methods like mind-mapping. tringent, tart, nutty) and to use the sticky properties of conventional flour. After revising food recipes, can affect the end-products. The concepts for innovative they can be scaled up for industrial processing. food recipes were developed Secondly, the chemical analyses underline the in a kitchen lab with samples The processing of walnuts comes with several difficulties. The tasting and sensory evaluations for potential of kernels as valuable food due to the and tested in terms of taste food products indicated the need for further tests for improving the marketability, durability, storage high oil and protein rates. Thus, kernels with and and sensory. capability, scalability and processability. It should be noted that walnut oil-containing products can without shells are used for oil production. For quickly become rancid. The products may contain a high number of harmful aflatoxins after impro- remaining oil press cakes, which can be used as Figure 9: Walnut Pasta /// Photo: AlpBioEco per storage. That means that careful treatment and refrigerated storage must be guaranteed for animal feed as well as for human nutrition, applies Walnut Pasta © AlpBioEco direct human consumption. Additionally, it is necessary to sort out and dispose mouldy and rotten the same protein composition (Bakkalbasi et al. nuts before processing. In order to make sure that no toxins are present in the end product, samples 2015). The following figure 8 shows four different should be analysed by laboratory tests. The use of walnuts in cosmetics produced some promising results. However, the developed recipes The analyses showed that walnut trees and should be fundamentally revised, consumer test conducted and legal requirements considered to their components have high potentials. The make it marketable. So far, it has not been possible to produce products with all desired properties walnut and its oil contain healthy simple and and which can maintain its state of aggregation over a long time. Furthermore, the amount and the unsaturated fatty acids as well as a variety of properties of the end products may vary significantly depending on the nature and quality of the minerals including calcium, iron, magnesium, used walnut part. For this reason, it is difficult to make general statements about their applicability phosphorus, potassium, sodium, zinc, thiamine, and basic recipes. riboflavin, niacin, folate, and vitamin A, B-6, B-12, C, D, E, K (USDA 2018). Currently, product designs are being optimised and other applications of walnut components are being investigated. In addition to the processing in the food industry, the use for papermaking and dyeing of textiles is of particular interest here, as leftovers from food production (leaves or shells) could be utilised here. Fig 8: Press cakes /// Photo: AlpBioEco Press cakes © AlpBioEco For transferring the knowledge from the sensory and chemical analyses, several tests ran in the kitchen lab with the following products: + Syrup from green walnuts & aroma extracts from leaves + Snack & energy balls with kernels, press cake + Pasta (fig. 9), noodles, dough & dumpling with press cake, oil, kernels or leaves + Spread sweet or salty with kernels, press cake, leaves or oil including slime + Milk & yoghurt from press cake + Dessert and ice cream with kernels, press cake + Extruding of press cake: tests are running Figure 10: Walnut paper /// Photo: Walnut paper © AlpBioEco + Extrusion-based 3D food printing (Procusini Pro+ of Print2Taste): sweet and savoury mass AlpBioEco Results and Replicable Roadmap AlpBioEco Results and Replicable Roadmap
16 17 2.3. SWOT walnuts 3. Herbs & 3.1. Market analysis herbs in general 2.3. SWOT WALNUTS 3. HERBS Internal factors External factors Herbs is a wide ranged field with various applications. The research targets at finding Strengths Weaknesses Opportunities Threats the bioeconomic needle in the haystack. This chapter focuses on cultivation, processing, refining and marketing aspects of herbs, with a closer look on Alpine hay (German • easy to cultivate (alter- • structure of cultivation • great demand, good price • maybe looser of climate ‘Magerheu’) in the Alpine Space. Raw materials and by-products were examined at the native for extensive (dispersed trees) • winner of the climate change (early shoot vulne- laboratory to discover new or unused potentials. orchards), no danger • lack of logistics change (tolerates dryness) rability towards late frost) of voles, deep-rooted, • price competition with tolerates heavy soils, • harvesting costs, proces- • undetected varieties/seed- sing, technologies (decen- lings with new characteris- other countries (with work occasionally soil-wetness • falling fruits get not tralised, manual work) tics/potential costs, degree of mechani- 3.1. MARKET ANALYSIS HERBS IN GENERAL zation) damaged • danger of late frost • diversity of products: • different pests leaves (insecticides, plant • new providers on global In order to get an overview of the market for the value chain of herbs, several information sources • broad diversity of varieties market (China, Kyrgyzstan), (fungus, fruit fly) protection), green nuts were used in line with the walnut value chain approach (see chapter 2.1). The analysis was conducted • well known, popular also with their research cultural assets, proudness • suitability for storage • less technical solutions, stepwise starting with a broad desktop research, followed by expert consultations and evaluation to potential for innovation • ‚niche issue‘ – not much on own trees if incorrectly handled, promotion & subsidies narrow down possible investigation directions in the laboratory analysis because the superordinated • interesting/valuable sensitivity for mold • substitution of imports market of herbs is not clearly demarcated. possible, demand exists • walnuts and oil get rancid substances • no advertising for medicine products • positive image (regional, • no consistent product Due to its great variety and small-scale • demand for wood from walnut trees possible (”popular” untreated) quality cultivation, the cultivation of herbs is poorly New trends and opportunities for developing new • some knowhow exists medicine) • organic products, a possi- • seedlings: difficult to achie- captured. Figure 11 shows the cultivation business models are related to the possibilities to: • less supply with seedlings bility for collective certifi- ve sufficient quantity (also with “IG Nuss” quality) of new area of herbs in respected countries. The + supply special varieties of different varieties cation (association) • contribution to biodiversity European demand for spices and herbs • no research on varieties for • some actors gain interest, • allelopathy: walnut tree + focus on product characteristics (insects, feed for winter- more than a century, alto- start-ups produces juglone bio- steadily grows due to trends like healthy time, early bloomer) (better colour & taste) gether a lack of research chemical that decreases living, medical uses and sustainability. • subsidies for organic • confederates (SlowFood, (for example biodiversity) growth of other organisms European buyers continuously look for new + customise according to buyer’s preferences cultivated permanent-crop www.mundraub.org) • multiple uses possible • erosion of knowledge • demand from (organic) local suppliers to meet the high demand + produce sustainably (organic, fair trade) (agro-forest-systems, (most specialists already bakeries and improve traceability. pasture) are pensioned) • cooperation with • widely consumed due • less knowledge about handicapped people For healthy living, unhealthy food ingredients such as salt, sugar and synthetic additives, are increa- to positive health effects numbers of trees / planta- • buildup of regional singly being replaced by natural ingredients like herbs. Another important health trend in Europe is (rich in polyunsaturated tions wild growing trees, varieties, characteristics know-how, competencies the intake of various protein products as an alternative to meat consumption and, in this sense, spices fatty acids-omega3 and omega6) • information about healthy • social elements (meeting and herbs play an important role in the food industry for the possibility to season and imitate the ingredients must be better point, neighbourhood taste of meat. The interest in the use of herbal substances and preparations for medicinal purposes is • walnut market constantly activities) growing, since walnuts distributed growing continuously worldwide. The interest in herbal products is mainly based on the assumption have beneficial properties • new applications in cosme- • lack of cooperation of better tolerability compared to synthetic drugs and sufficient efficacy. This is due to the long on health (+53,3 % from between professional tics, other areas: antibac- terizid tradition of natural medicine and the frequent belief that herbal and medicinal products are natural 2000 to 2010) institutions and producers (growers) • restoring orchards: and safe if compared to synthetic pharmaceuticals. • walnut leaves: adstringent, hyperemic, antioxidant, renovation with modern antibacterial varieties (contemporary Figure 11: assortment) Cultivation areas of herbs – • deep root tree, consists localised experts‘ assessments deep ground & occasional • woodworking/ stagnant moisture carpentry: very hard, used • traditional specialities for premium & highly (recipes) valued furniture, one most expensive wood • environmentally-friendly form of cultivation, well- established and widely accepted Table 5: SWOT Walnuts 2.4. CONCLUSION The analyses show excellent potential for walnuts within the bioeconomic framework in the laboratory and the markets. However, the lack of know-how, logistics, unknown types of walnuts and the vulnerability of the nut are significant obstacles for creating an effective strategy for taking advantage of the promising opportunities related with this tree. A proper strategy, long-term relations between farmers and producers through associations and developing well-defined value chains could overcome these problems. Further information can be found in the download section on the AlpBioEco-homepage. AlpBioEco Results and Replicable Roadmap AlpBioEco Results and Replicable Roadmap
18 19 3.1. Herbs in general 3.1 Herbs in general & 3.2. Laboratory analysis The demand for certified cosmetic ingredients is growing. The popularity of organic (mostly with the New products based on herbal by-products New processes COSMOS standard) and fairtrade certifications are also driven by the use of these certifications in food products. Germany, France and the United Kingdom also have the highest number of natural product launches and a strong focus on innovation. Cosmetic companies put substantial effort into research for + paper + fl oral waters for seed tanning in organic + new textile products from nettle or broom agriculture, farms new natural ingredient coming from plants that will replace some synthetic molecules. + cosmetic products with antioxidants + essential oils as herbicide-antimicrobial-fungicide It can be seen that bioeconomic possibilities for herbs and their residues are, in particular, the utilisation + nutraceutical products with active ingredients + essential oil as food preservatives of plant components for biogas plants, as fertiliser, the production of paper or textile material from plant + novel ingredient from fungicide or herbicide + composting of solid residues from by-products fibres. The respondents to the market study showed that they are not highly activated by producers or + new edible plants direct human consumption processors. Only the possibility of using raw materials for biogas plants is used more frequently. The experts stated that they have currently no interest in recycling waste residues because it is not econo- Table 6: New possibilities for Alpine herbs mically feasible yet (due to transport costs, personnel costs). Furthermore, there are currently no struc- tures for the purchase of waste residues, possibly because small amounts are used for composting. The results of the market study also indicate that herb producers know little about the bioeconomic potential. 3.2. LABORATORY ANALYSIS The market for herbs in the Alpine Space is a niche market with mainly small-scale cultivation areas and One of the common industrial processes in the researched area is the distillation of herbs for essential oils small producers mainly using their equipment. The market for aromatic and medicinal herbs (especially as described in Figure 12. for dried herbs) is characterised by strong competitive pressure and a high price pressure because of the buyers‘ power. Also, the cultivation of herbs is cost-intense due to the need for specific technology. 1 2 Condenser 3 Separator Despite these challenging market conditions, the identified potentials along the value chain are rarely C° exploited within the growing regions (e.g. quality orientation, product diversification, collecting of wild +++ Result A herbs, cultivation of Alpine-specific herbs). The exploitation of the potentials differs in the countries Essential oil of the Alpine Space. In South Tyrol, for example, can be found other applications than in Slovenia. The Delicate survey experts mentioned various (innovation) approaches that could be used to activate unused flower petals C° value creation potentials. in water - Water and Condenser essential oils Taken the whole Alpine Space into account, the following four types of cools Result B the steam activities can be seen as particularly supportive: Heat Floral water + establishment of joint quality guidelines by producers & processors that ensure a Figure 12: Distillation process high-quality-standard of Alpine herbs Moreover, essential oils only recently began to be investigated regarding their biological activities and + supporting cooperation with regional organisations, umbrella brands & other relevant possible alternative uses. Essential oils could be considered as antimicrobial agents enabling a decrease or elimination of preservatives in the cosmetic formulation (Lesage-Meessen, 2015). Moreover, several market players to improve a supra-regional marketing studies have pointed out the possibility to use essential oils and their components in medical and plant pathology as well as in the food industry for the control of micro-organisms pathogenic to consumers + supporting communication between the market players to identify region-specific and responsible for food spoilage (Cantore, 2009). With the process for essential oils, floral waters (called innovation potentials & possibilities for their joint implementation hydrolates) and solid wastes occur as by-products. A bibliographic study was done by taking into consi- deration scientific articles on hydrolates and essential oils from different types of Alpine plants. + involvement of machine manufacturers (harvesters, seeders, processing machines) to get information about new technological standards & their implementation possibilities This analysis highlights that hydrolates seem to be promising for innovative uses since they still have active molecules: +w ater extract of Rosemary – Rosmarinus Officinalis (Wollinger, 2016) entails rosmarinic acid There is a variety of species of herbs and different cultivation processes in the Alpine Space, for this reason, a multi-approach methodology has been used to set the criteria for the definition of herbs that has strong antibacterial activities, usable both in food industries as well as excipient and the evaluation of the relative typologies of laboratory analysis. A preliminary questionnaire has ingredient in cosmetic & nutraceutical industries been prepared and fulfilled with information on typologies of herbs, distribution, types of cultivation, use and companies by all PPs in order to give a regional overview of the herbs value chain. +w ater extract of Lavander – Lavandula Angustifolia (Śmigielski, 2015) entails linalool & linalyl The results obtained from the market analysis revealed the actual uses of herbs, the actors and the acetate that have good antibacterial & antifungal activities value chain organisation together with the most common industrial processes and related by-pro- ducts or wastes. Moreover, an in-depth scientific literature analysis was done for the evaluation of + Mentha Piperita extracts (Meng, 2008), show concentration of antioxidants & functional ac- their potential for the future bio-based application. Throughout these analyses, possible new scenarios tive compounds that prevent the propagation of the lipid peroxidation process in a complex in the value chain of herbs have been hypothesised with new products and processes. These possibilities are described in the following table 6. lipid matrix, such as a foodstuff, preservative; it is feasible for seeds tanning in organic agriculture due to their antibacterial activities as substitute of copper AlpBioEco Results and Replicable Roadmap AlpBioEco Results and Replicable Roadmap
20 21 3.2. Laboratory analysis 3.2. Laboratory analysis On this basis, a French farm distillery could be identified which produces 27 floral waters, coming Plants Essentail Oil/Hydrosol Compounds Specific Activities References from the essential oil distillation process. Samples were analysed for the evaluation of the total antioxidant activities (by DPPH assay) and the phytochemical profile for the determination of the chillea millefolium/ A 1,8-cineole, Alpha-thujone, Azulene Antioxidant activity Eghdami et al. (2010) main families of compounds. For all the samples, antioxidant activity was not revealed with DPPH Yarrow assay on the sample as it is (in contrast with scientific literature), but three different categories of compounds (sugars, peptides and non-polar compounds) were found for all the samples. Only in the case of Artemisia vulgaris hydrosols a different methodology was used to concentrate the Artemisia vulgaris/ 1,8-cineole, sabinene, alpha-thujone, Antioxidant activity Blagojevic et al. constituting molecules for the phytochemistry of the extract for the subsequent HPLC analysis. Mugwort alpha-caryophyllene (2006); Temraz et al. (2008) In this case, the concentrated hydrosol revealed the content of polyphenols and flavonoids. Centaurea cyanus/ Hydrosol: Chlorogenic, caffeic, Anti-hemolytic, anti- Bragueto Escher A different methodology was used for evaluating the presence of active molecules in Lavender Cornflower ferulic, p-coumaric acids, hypertensive activity in et al. (2018) water flowers without the concentration step. The gas-chromatography-mass spectrometry isoquercitrin, coumarin vitro; antioxidant activity (GC-MS) is an analytical method that combines the features of gas-chromatography and mass spectrometry to identify different substances. This analysis reveals that still linalool together with Juniperus oxycedrus/ a-pinene, myrcene, limonene, Antifungal and Salido et al. (2002); 1,8-Cineole (eucalyptol) and borneol are present in the hydrolysate. These compounds are very Cade germacrene D, gamma-muurolene antioxidant activity Cavaleiro et al. interesting for their use as a scent in perfumed hygiene products and their strong activities as (2005); Chaouche et al. (2015) an insect repellent (see figure 13). Daucus carota ssp. geranyl acetate, Alpha- pinene/ Hydro- Antifungal activity Zatla et al. (2017) Maximus/ Daucus sol: Root hydrosol: myristicine, (E)-me- Food preservative carota thyl-iso-eugenol, methyl-eugenol. Aerial parts hydrosol: caryophyllene oxide, p-cymene-8-ol Inject repellent Cedrus atlantica/ alpha-pinene, Himachalene, Cadinene Bactericidal activity Derwich et al. (2010) Cedar branch Cupressus semper- Alpha-pinene, Cedrol Moderate antibacterial, Mazari et al. (2010) virens/ Cupressus antifungal activities Fungicide branch herbicide Juniperus communis/ Alpha-pinene, myrcene, sabinene, Antioxidant activity Höferl et al. (2014) Juniperus branch limonene, Beta-pinene Organic and berry ESSENTIAL OIL Cosmetic FLORAL WATERS seed products tanning Helichrysum Alpha-pinene, a-cedrene, aromadendre- Antimicrobial activities, Mastelic et al. (2005) italicum/ ne, Beta-caryophyllene, limonene, neryl in paticular against Helichrysum acetate, 2-methylcyclohexyl pentanoate Staphylococcus aureus & octanoate, geranyl acetate & Candida albicans Figure 13: By-products from distillation /// Photo: Bottles with rosemary oil on white background © Pixel-Shot /// Photo: flask with green liquid isolated – illustration © Wth Hyssopus officinalis/ isopinocamphone, (-)-Beta-pinene, Moderate antioxidant Venditti et al. (2015); Hyssop (-)-terpinen-4-ol, pinocarvone, carvacrol, & antimicrobial activity, Aazza et al. (2012); p-cymene, myrtenal potential chemopreven- Kizil et al. (2010) For completing this study, a review on the typologies of active molecules in hydrolates and the tive & immunostimulant essential oils was done. For every single plant, the most relevant articles were evaluated by taking effects into consideration the pedo-climatic zone of the plants for giving pertinent information for the Alpine Space territories. A comprehensive overview of the main active molecules found in the Lavandula angustifolia linalool, linalyl acetate, borneol/ Antimicrobial and Śmigielski et al. & cl./ Fine & Maillette Hydrosol: linalool, borneol antioxidant properties. (2013); Prusinowska essential oils and the hydrolates as well as the specific activity together with the references can Lavender et al. (2015) be found in the following table 7. Lavandula hybrida linalyl acetate, linalool/ Antimicrobial, Baydar et al. (2013) abrialis & super/ Hydrosol: Linalool antioxidant properties Lavandin Abrialis & super Origanum majorana/ terpinen-4-ol, cis-sabinene hydrate, Antioxidant, free radical Méabed et al. (2018); Marjoram p-cymene, -terpinene/ Hydrosol: scavenging activities Vera et al. (1999) Chlorogenic, Rutin, Ferulic acid Table 7 – Part 1: Main active molecules found in essential oils and hydrolates AlpBioEco Results and Replicable Roadmap AlpBioEco Results and Replicable Roadmap
22 23 3.2. Laboratory analysis 3.3. SWOT herbs 3.3. SWOT HERBS Plants Essentail Oil/Hydrosol Compounds Specific Activities References Internal factors External factors Mentha x piperita/ limonene, Carvone D/ Hydrosol: Antioxidant properties De Sousa Barros Strengths Weaknesses Opportunities Threats Peppermint 1,8-cineole, Menthone, Menthol et al. (2015); Amr E. Edris (2009); • high production: almost • increased disease in laven- • increasing demand for • production must be Dorman et al. (2003) 20,000 ha lavender, 2,500 der 85 % of the acreage essential oils adapted to climate change ha clary sage makes up a variety • significant development • impact of regulatory Hypericum perfora- nonane, p-cymene, 3-methylnonane Antibacterial activity Saddiqe et al. (2010); • high export share • competition from Bulgaria of new markets requirements (range) tum/ Hypericum Rančić et al. (2005) perfume plants of essential oils on cloned lavender: tend to • high demand for products • damage caused by • professionalised produc- lower prices drought • new types of lavender, tion, processing, quality • helpers needed for manual more resistant to diseases • overproduction of clary Perilla frutescens/ 2-furyl methyl ketone, decahydro1- Insecticidal toxicity and Chun Xue You control along value chain flower harvesting sage: price drops • positive external effects: Perilla Shiso methyl-2-methylene-naphthalene, antioxidant activity et al. (2014); • efficient distillation • small-scale farms tourism, lavender honey, • price fluctuations limonene, caryophyllene Meng et al. (2009) through innovations: • scarce associationism organic, herbal cosmetics reduction of energy among producers leads • linking tourism (sales & Rosmarinus alpha-pinene, 1,8-cineole, Linalool Radical-scavenging, Gachkar et al. (2006); losses, better profitability to fragmented offers awareness raising / herbal officinalis/ Rosemary antioxidant properties Deok Hyeon Jeon et of distillation process • few species cultivated & education) al. (2013); Wollinger • altitudes optimal for insufficient quantity to • alternative source of et al. (2016) cultivation of different meet demand income for mountain herb varieties (Alpine to • potential water shortages farmers Mediterranean) Santolina artemisia ketone, camphor, Demirci et al. (200) chamaecyparissus/ beta-phellandrene Santoline • professional production • high production costs & • strong traditional markets • international competition structures: cooperatives, for new producers • new markets in develop- • competitive foreign Satureja montana/ thymol, geraniol Antimicrobial activity Ćavar et al. (2008) large companies, plant • necessary imports to meet ment (plant extracts, production costs Savory development (especially demand nutritional supplements), • price fluctuations aromatic plants fresh, frozen plants) identified future uses • limited plant protection Salvia officinalis/ 1,8-cineole, Alpha-thujone, Bacteriostatic and Chalchat et al. (1998); • high production growth methods, high demand for • consumer expectations: Sage officinalis Beta-thujone, Camphor, bactericidal activities Delamare et al. • good quality assurance alternatives local production, high Alpha-Humulene (2007); Aazza et al. traceability • increasing organic pro- • increasingly strict (2011) duction of aromatic plants regulations • linking tourism (sales & • strong tradition of gather- • see last 5 points of awareness raising / herbal Salvia sclarea/ linalyl acetate, linalool, geranyl Antifungal activity Pitarokili et al. (2002) ing, using medicinal and perfume plants education) Clary Sage acetate, R-terpineol aromatic herbs (SLO) • alternative source of • see last points of income for mountain Thymus vulgaris thymol, p-cymene, Carvacrol Antimicrobial activity Rota et al. (2008) perfume plants farmers linaloliferum/ Thyme • increasing poppy • high production costs • see aromatic plants • international competition production • imports to meet demand • linking tourism (sales, • other production Table 7 – Part 2: Main active molecules found in essential oils and hydrolates • high proportion of land in • limited crop protection awareness raising/herbal methods: fermenters organic farming methods, high need for education) or biotechnology • professional production, alternative methods • alternative source of • increasingly stringent processing structures • possibility of using me- income for mountain regulations: pesticide along the value chain dicinal plant extracts as farmers residues, pyrrolizidine • strong tradition of gather- natural insecticides alkaloids; Nagoya Protocol: restriction of research, medicinal plants ing and using medicinal • renewal of farmers have and aromatic herbs (SLO) regulatory threats related high-cost investments to development of • 41 % of area planted • see last 4 points of standards: hygiene, with medicinal plants perfume plants health, quality is organic (IT) • price fluctuations • medicinal plants and extracts are widely used in food, -supplements, body care and pharma- ceutical products • 41 % of the area planted with medicinal plants is organic (IT) • see last points of perfume plants Table 8: SWOT Herbs AlpBioEco Results and Replicable Roadmap AlpBioEco Results and Replicable Roadmap
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