YOU CULTURE - WE CARE! - EPPENDORF INFOCUS | CELL CARE - PREMIUM SOLUTIONS FOR CELL APPLICATIONS
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Eppendorf InFocus | Cell Care ‡ Sterility ‡ Reproducibility ‡ Performance You Culture – We Care! Premium solutions for cell applications
The Knowledge to Nurture. Eppendorf InFocus Cell Care Cell biology is more than just another scientific Working with cells means to care. There are many For laboratories looking to assure the quality of discipline; it is one of the most important areas risk factors associated with the cell culturing their cells, improve overall efficiency, and streamline of life science research, and one of the fastest process. These risk factors can alter the quality the analysis process, Eppendorf provides com- evolving fields with groundbreaking discoveries of cells and must be avoided. Ensuring a sterile prehensive laboratory technologies ranging from taking place almost every day. work environment and avoiding sample liquid handling tools and systems, to sample contamination are some of the many everyday handling solutions, to cell handling equipment. With each new insight, significant intellectual challenges for cell culture. and practical challenges remain and confront As a leading life science company we aim to use researchers. Developing new methods of manipulating cells our extensive knowledge and competence to and analyzing results demands trusted research develop, manufacture and provide researchers methods and reliable tools that support indisputable the best tools and systems for every step of the results. cell culture process. Dr. Ben Davies, Transgenic Core Head/Group Head, Wellcome Trust Centre for Human Genetics, Oxford, UK “We work with unique, genetically modified stem cell clones. As a result, ensuring that culture conditions are optimal and reproducible are the most important priorities for our cell culture laboratory.” 2
Eppendorf InFocus | Cell Care Whether you are studying functional biology in academic research, pursuing cell-based drug discovery in large-scale industrial laboratories or anything in between, Eppendorf delivers solutions that will help you obtain the most reliable results, and to work more productively and efficiently. Dr. Nicole Kühl, Head of PromoCell Academy and teacher Dr. Mark Slack, Group Leader Cellular Assays at Evotec, in cell culture courses, Heidelberg, Germany Hamburg, Germany “There are many prerequisites to avoid contamination of cell “We work with various primary cell lines and run a lot of a culture – antibiotics should not be one of them.” cell-based high-throughput screens. To obtain reliable results, I need to rely on repeatable working conditions. Therefore reproducibility is very important for me.” 3 4 The Right Tools, Every Step of the Way. Eppendorf InFocus Cell Care:
‡ Growing Cells ‡ Manual Liquid Handling Reliability and advanced control are Outstanding precision and accuracy, the hallmarks of New Brunswick CO2 reduced risk of contamination, incubators, biological shakers, and ergonomic design and easy handling cell culture bioreactors. are the key features of our pipettes, pipettors and dispensers. Cu ed lt ur Se e ‡ Automated Liquid Handling ‡ Mixing, Heating and Cooling epMotion 5070 CB, designed to fit Our Thermo/Mixer family: high precision on a cell culture bench, is the and reliability in its performance, ideal tool for automated pipetting together with a broad selection of of cell culture applications. exchangeable thermoblocks, build a helpful tool for cell analysis. Passage Store ‡ Freezers ‡ Transfection Keep your sample safe, secure Cell manipulation at its best: whether and easily accessible with the energy you want to transfect, electrofuse and space saving New Brunswick or micromanipulate your cells, Innova and Premium ULT freezers. we have the ideal solution for gentle treatment of your cells. M an ze ip u a l y lat An e ‡ Analysis ‡ Centrifugation Fast and accurate nucleic acid The right tools, eve ry step of the way. Our centrifuges provide high quantification and amplification temperature accuracy, speed with easy to use Instruments from and applicational flexibility. Eppendorf. Benefit from preserved sample integrity and reliable results. ‡ epServices ‡ Consumables for Analysis Enjoy peace of mind with technical, Almost 50 years experience in applicational and quality services manufacturing laboratory consumables for your Eppendorf instruments are the foundation for the outstanding by the people who made them. quality, purity and performance Eppendorf InFocus | Cell Care of Eppendorf consumables.
Eppendorf InFocus | Cell Care We Care for You … Preserve sterility and prevent contamination. Optimize cell handling at all times. To minimize the time cells are exposed to non- Preventing contamination in the cell culture lab Cell culture requires appropriate temperatures and physiological conditions, the steps for changing is essential. High-purity consumables and gas mixtures that resemble physiological conditions. media and seeding cells are facilitated and products that can be easily cleaned are important A change in the environment can result in different accelerated by in maintaining a sterile work environment. phenotypes being expressed; for example, when the Whether you are studying functional biology in media is changed or the cells are passaged. ‡ Our heated centrifuge 5702 RH – keeps cells at academic research, pursuing cell-based drug ‡ Aerosol-tight rotors in Eppendorf Centrifuges physiological temperatures during centrifugation discovery in large-scale industrial laboratories provide protection against contamination and can ‡ Multiple inner-glass door option on steps. or anything in between, Eppendorf delivers be fully autoclaved – for sterilization. New Brunswick Galaxy CO2 incubators ‡ Multipette plus in combination with Combitips solutions that will help you obtain the most ‡ Each batch of the Biopur purity standard is preserve chamber temperature and gases – provides contamination-free dispensing of reliable results, and to work more productively controlled and certified by an independent during sample access. volumes in long series. and efficiently. laboratory – ensuring the highest safety. ‡ The lightweight, hand-held Easypet – offers ‡ ep Dualfilter T.I.P.S. feature a unique two-phase accurate pipetting with adjustable speed and filter – effectively protects pipettes and prevents fine control of the meniscus movement. cross-contamination of samples. ‡ epMotion 5070 CB automated pipetting system – ‡ Eppendorf pipettes are UV resistant and optimized for cell culture applications – fits inside autoclavable – for secure decontamination. a laminar flow or fume hood. ‡ New Brunswick Galaxy CO2 Incubators feature a unique fanless design and seamless, weld-free chamber – to prevent contamination and simplify cleaning. Dr. Nicole Kühl, Head of PromoCell Academy and teacher Dr. Mark Slack, Group Leader Cellular Assays at Evotec, in cell culture courses, Heidelberg, Germany Hamburg, Germany “There are many prerequisites to avoid contamination of cell “We work with various primary cell lines and run a lot of a culture – antibiotics should not be one of them.” cell-based high-throughput screens. To obtain reliable results, I need to rely on repeatable working conditions. Therefore reproducibility is very important for me.” 6 3 5 4 your research results. The Right Tools, Every Step of the Way. instruments are all crucial factors that can influence as well as investments in quality consumables and lab adheres to stringent best practices. to detail at every step of the cell culture process, it’s essential to ensure that everyone in your Eppendorf InFocus Cell Care: Proper laboratory set-up and procedures, attention Cell culture is critical to your success, so
We Care for You … Preserve sterility and prevent contamination. Optimize cell handling at all times. To minimize the time cells are exposed to non- Preventing contamination in the cell culture lab Cell culture requires appropriate temperatures and physiological conditions, the steps for changing is essential. High-purity consumables and gas mixtures that resemble physiological conditions. media and seeding cells are facilitated and products that can be easily cleaned are important A change in the environment can result in different accelerated by in maintaining a sterile work environment. phenotypes being expressed; for example, when the media is changed or the cells are passaged. ‡ Our heated centrifuge 5702 RH – keeps cells at ‡ Aerosol-tight rotors in Eppendorf Centrifuges physiological temperatures during centrifugation provide protection against contamination and can ‡ Multiple inner-glass door option on steps. be fully autoclaved – for sterilization. New Brunswick Galaxy CO2 incubators ‡ Multipette plus in combination with Combitips ‡ Each batch of the Biopur purity standard is preserve chamber temperature and gases – provides contamination-free dispensing of controlled and certified by an independent during sample access. volumes in long series. laboratory – ensuring the highest safety. ‡ The lightweight, hand-held Easypet – offers ‡ ep Dualfilter T.I.P.S. feature a unique two-phase accurate pipetting with adjustable speed and filter – effectively protects pipettes and prevents fine control of the meniscus movement. cross-contamination of samples. ‡ epMotion 5070 CB automated pipetting system – ‡ Eppendorf pipettes are UV resistant and optimized for cell culture applications – fits inside autoclavable – for secure decontamination. a laminar flow or fume hood. ‡ New Brunswick Galaxy CO2 Incubators feature a unique fanless design and seamless, weld-free chamber – to prevent contamination and simplify cleaning. 6 5 your research results. instruments are all crucial factors that can influence as well as investments in quality consumables and lab adheres to stringent best practices. to detail at every step of the cell culture process, it’s essential to ensure that everyone in your Proper laboratory set-up and procedures, attention Cell culture is critical to your success, so
… Caring for Your Cells. No more sample mix-ups. Eppendorf also offers a wide range of consumables Minimize sample loss. As anyone working in a lab can attest, mistakes to support the cell handling workflow such as deep- Research at the single-cell-level to fully immerse happen – even in cell culture work: From incorrect well plates, microplates and microcentrifuge tubes. into cellular pathways and the use of ex-vivo material sample tracking, well-to-well cross contamination, are nowadays established methods in life science to mis-loading of microplate formats and sample ‡ OptiTrack Matrix, a contrast rich alpha- research. Invaluable samples undergo routine swaps. These issues can be minimized through numeric coding of plates – provides fast and workflows and there is a strong need for quality proper procedures and use of the best technologies secure well tracking. tools tailored to minimize sample loss. Eppendorf and tools. Eppendorf consumables and automated ‡ Laser engraved inscription – ensures high meets this need with its tradition to ensure the liquid handling systems help to eliminate the chemical and UV resistance. highest reliability of tools and the willingness risk of sample mix-up, preserving the integrity of ‡ Wide variety of colors – facilitates lab to implement new technologies that best fit the your cell culture work. organization. ever-changing needs of researchers. ‡ Suitable labeling surfaces – allow for safe The epMotion 5070 CB, designed to fit on a labeling or barcoding. ‡ Outstanding Eppendorf pipettes combined in a cell culture bench, is the ideal automated pipetting ‡ Optimized sealing system – prevents well-to-well system with epT.I.P.S. – ensure high precision system for cell culture applications. cross contamination. during liquid handling workflow processes. ‡ Eppendorf Deepwell Plates with RecoverMax ‡ Automated precision workflow – eliminates design – for maximum sample recovery. well-to-well cross contamination and mis-loading ‡ Eppendorf LoBind tubes and plates – provide of microplates. optimal yield of DNA and protein samples derived ‡ Programmed liquid handling tasks – minimize from limited material. human errors. ‡ epT.I.P.S. LoRetention – prevent loss of valuable ‡ Optical sensor identifies plate orientation – samples when pipetting detergent solutions. assures correct worktable setup and sample volumes. Eppendorf InFocus | Cell Care 7
Eppendorf InFocus | Cell Care Focus on the Essentials! eppendorf®, epMotion®, Eppendorf Thermomixer®, epT.I.P.S.®, Eppendorf Multipette®, Eppendorf Combitips®, Easypet®, OptiTrack®, RecoverMax®, New Brunswick is a subsidiary of the Eppendorf Group. New Brunswick is a trademark used by New Brunswick. Eppendorf Biopur® and Eppendorf LoBind® are registered trademarks of Eppendorf AG, Hamburg, Germany. epServices™ and Eppendorf Deepwell Plates™ are trademarks of Eppendorf AG, Hamburg, Germany. All rights reserved including graphics and images. Copyright © 2011 by Eppendorf AG. Order no. AQ22312 020/GB2/5T/0911/FEEL · Printed climate neutrally in Germany Further information available at www.eppendorf.com/cellcare Your local distributor: www.eppendorf.com/worldwide Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 53801-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA Tel: +1 516 334 7500 · Toll free phone: +1 800-645-3050 · Fax: +1 516 334 7506 · E-mail: info@eppendorf.com Application Support Europe: Tel: +49 1803 666 789 (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt. Festnetz; Mobilfunkhöchstpreis 42 ct/min) E-mail: support@eppendorf.com North America: Tel: +1 800 645 3050 · E-mail: techserv@eppendorf.com Asia Pacific: Tel: +60 3 8023 6869 · E-mail: support_asiapacific@eppendorf.com
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