THE GROWTH AND YIELD OF RICE AFFECTED BY THE APPLICATION OF LIME, ORGANIC COMBINED WITH INORGANIC FERTILIZER DURING TWO CROPS OF 2020-2021
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Turkish Journal of Physiotherapy and Rehabilitation; 32(3) ISSN 2651-4451 | e-ISSN 2651-446X THE GROWTH AND YIELD OF RICE AFFECTED BY THE APPLICATION OF LIME, ORGANIC COMBINED WITH INORGANIC FERTILIZER DURING TWO CROPS OF 2020-2021 Nguyen Van Chuong1, Le Ngoc Tuan2 1 Department of Crop Science, Faculty of Agricultural, 2 Master student of crop science 1,2 An Giang University, Vietnam-Vietnam National University, Ho Chi Minh City, Viet Nam, 18 Ung Van Khiem St., Long Xuyen city, An Giang province 880000, Vietnam 1 nvchuong@agu.edu.vn ABSTRACT: The aim. Overuse of inorganic fertilizers has significantly been its negative effects on the soil health, environment and crop yield. Co-appliation of cow manure, lime and mineral organic fertilizer (Vian), which has been organic fertilizer sources replaced to reduce the use of inorganic fertilizers, environmental pollution and ensure sustainable crop production in agriculture.Materials and methods.This studyevaluate the effects of lime, cow manure, Vian with inorganic fertilizers on the growth and yield of rice. A field experiment, which was executed during two crops of 2020-2021 in ChauThanh district, An Giang province included the NPK application (85kgN-45 kgP2O5 -45 kgK2O per ha) of four treatments with four replications for both crops. In the Winter-spring (crop1), different rates of lime, Vian and cow manure were applied by the control-T1 (0), T2 (500 kg CaO/ ha), T3 (320 kg Vian/ ha) and T4 (10 tons cow manure per ha). On the prior experimental sites of Winter-Spring crop were laid by the NPK application four treatments in the Summer-summer-autumn crop (crop 2). Results. Co-application of cow manure, Vian as well as lime with NPK raised positive effects on yield composition, quality and yield of rice Jasmine85 in the crop 2.The amendment of 10 tons cow manure with 85 kgN - 45 kgP2O5 - 45 kgK2O per ha was maximum values of yield components, yields, grain protein, grain lipide of rice in crop 2. The rice Jasmine85 yield of the cow manure combined with NPK treatment raised24.5% compared to the NPK treatment in crop 2. Conclusions. The effect of cow manure was clearly showned after one rice crop and lime has been remained until crop 2.The further studies of different lime ratios with different organic fertilizers are carried out to draw valid conclusions. Keywords: Cow manure, lime, Vian, rice Jasmine85, yield. nutrient uptake. I. INTRODUCTION The continuous flooding cultivation of rice has led to soil degradation, increased plant diseases and rice yield.The crop intensification and increase of rice, which has been one of the reasons for reducing the soil fertility, increasing pathogens and weeds reduced the quality and productivity of rice (Thuc et al., 2021).Although inorganic fertilizers used to raise the level of nutrients in the soil. The nitrogen, phosphorus and potassium nutrients, which required for plant growth have applied to the soil in the form of chemical fertilizers. However, There are significantly disadvantages of inorganic fertilizers compared to naturally organic fertilizers. It produced several acids (HCl, H2SO4) that reduced soil pH and soil porosity(Ning et al., 2017). Organic amendments, which have been a positive method to optimize the use of inorganic fertilizers in rice cultivation decreased pathogens, pests and weeds and increased soil fertility and rice yield (Bolan and Duraisamy, 2015).The organic fertilizers, which content the high organic matter and nutritional elements improve the physical and chemical properties of the soil. It can also improve the biological and biochemical properties of the soil and optimize the microbial community in the soil structure (Mitu et al., 2017).the toxicity of aluminum and iron that restricts the plant growth exists on acidic soils. Co-application of lime and manure fertilizers, which both increases soil pH and decreases Absorption of heavy metals from the soil to plants helps the well plant growth and high yield (Chuong et al., 2021; Mitu et al., 2017). This study, the incorporation of lime, cow manure, mineral organic and www.turkjphysiotherrehabil.org 6786
Turkish Journal of Physiotherapy and Rehabilitation; 32(3) ISSN 2651-4451 | e-ISSN 2651-446X NPK fertilizer that was applied during both rice crops of 2020 -2021 investigated effects of organic fertilizer and lime amendment on the growth and yield of rice Jasmine85. II. MATERIALS AND METHODS Fertilizers and Seed collection Cow manure, with was significantly dried and mixed with Trichodermaspp. applied before 15days before sowing. The rice Jasmin85 and mineral organic fertilizer (Vian) of LocTroi group was used during both crops. The cow manure, which was purchased from local farmers mixed Trichodermasp. to decompose. Cow manure and lime were applied fifteen days before rice seeding. Vian and NPK were applied at stages -1, 6, 17, 37 and 6, 17, 37, 48 days after seeding, respectively. Location site and experimental structure: Fourfield treatments and four replications were performed during Winter-Spring and Summer-Autumn crops of 2020-2021 at ChauThanh district, An Giang province, Vietnam. Total experimental area was 320 m2(each plot area was 20 m2x 4 replications x 4 treatments = 320 m2). Cow manure was significantly decomposed by Trichoderma sp.Application of chemical fertilizer (NPK) combined with Cow manure (CM), mineral organic fertilizer (Vian) and lime (CaO) amendment, which was only used in the season 1 (Winter-Spring) was not used in the season 2 (Summer-Autumn).In the Summer-Autumn crop, all treatments that were only applied the NPK fertilizer repeated on prior experiment sites. The experimental setup of Winter-Spring crop has four treatments with four repeats: The control-P1(85 kgN - 45 kgP2O5 – 45 kgK2O per ha; P2- 0.5 ton CaO + NPK;P3- 320 kg Vian + NPK; P4-10 tons CM. The Summer-Autumn season (crop 2), the prior treatment sites of crop 1 were repeated for crop 2. All treatments of crop 2 applied NPK (Without CM, Vian and lime)(Table 1). Table 1. All different fertilizers of treatments from to crop 1 to crop 2 Fertilizers Inorganic Organic Lime Mineral organic Treatments (kg/ha) (ton/ha) Nitrogen Phosphorus Potassium Cow manure CaO Vian Winter-Spring Control (T1) 85 45 45 0 0 0 T2 85 45 45 0 0.5 0 T3 85 45 45 0 0 0.32 T4 85 45 45 10.0 0 0 Summer-Autumn Control (T1) 85 45 45 0 0 0 T2 85 45 45 0 0 0 T3 85 45 45 0 0 0.32 T4 85 45 45 0 0 0 Table 2.physical and chemical characteristicof Soil before the experiment at 20 cm depth Parameters Value Parameters Value Silt (%) 30.5 Total N (%) 0.276 Clay (%) 64.0 Available P (ppm) 14.3 Sand 5.5 Exchangeable K (ppm) 178 Textural class Silt clay Loam Organic matter (%) 4.69 pH 5.5 C/N ratio 9.85 Collection of Soil and crop samples Soil samples that were collected before and after the experiment for each cropanalysed at the central laboratory of An Giang university. The rice growth data, which were the number of tillers and plant height determined at 15, 35, 55 and 100 days after sowing. the area of each frame, which was 4 × 0.5 m2were counted for tillers. Plant heights that were measured average from 10 plants per plot selected during 15, 35, 55 and 100 days after sowing. Yield component (filled and unfilled, 1000-grain weight, moisture) was measured average the whole panicles per m2.The rice yield was measured tonnes of rice grains per ha at 14% of moisture content. Soil chemical parameters that are www.turkjphysiotherrehabil.org 6787
Turkish Journal of Physiotherapy and Rehabilitation; 32(3) ISSN 2651-4451 | e-ISSN 2651-446X pH, organic matter, total nitrogen, available phosphorus and exchangeble potassium, which analyzed by the AOAC and the Bray–Krutz method were the most suitable for determination methods of the laboratory(Wayne Sabbe, 1980, Bray and Kurtz, 1945). Results of soil physical and chemical properties were presented in Table 2. Statistical Analysis The analysis of variance (ANOVA) was done by Statgraphics Centurion XVII. Means of variance that was separated by Duncan’s Multiple Range Test was represented at Pvalue= 0.05probability distribution. III. RESULTS AND DISCUSSION Agronomic characteristics Plant height Table 3.The rice Jasmine85 height (cm) in both cropsof 2020-2021 Days after seeding (DAS) Treatments 15 35 55 100 Winter-Spring(A) T1 33.8 64.6a 76.9ab 86,1ab T2 33.7 65.4a 74.8a 84.7a T3 33.5 67.7a 78.0ab 89.5ab T4 35.3 72.6b 80.2b 90.8b Summer-Autumn(B) T1 32.4 54.3a 65.9a 80.5a T2 32.7 56.4a 68.9ab 81.7ab T3 34.3 57.9a 73.8c 86,7bc T4 34.8 66.5b 73.5bc 87.8c F(A) ns ** ** ** F(B) ns ** ** ** CVA(%) 5.10 5.26 4.20 4.47 CVB(%) 5.64 9.61 5.98 5.29 ns: insignificant differencesat the Pvalue=0.05; **significant differencesat the Pvalue= 0.01 The results of Table 3 proved that rice Jasmine85 heights were significantly different at 1% during growth stages except for the first stage(15 DAS) was insignificant. Co-application treatments of lime, cow manure, Vian with NPK had higher plant height than the control treatment during development stages of rice in both crops. The amendment treatments of lime, cow manure and Vian, which impacted clearly the rice height during the rice growth of two crops developed slowly at 15 days after seeding. The growth of the rice height is related by environment factors such as water, fertilizer, soil, farming techniques. However, fertilizers is one of the most important factors. Nowadays, there are different types of fertilizers and rice applied techniques that have affected on the growth and yield of rice (Anas et al., 2020; De Ngoc Nguyen et al., 2017). Table 3.The stillers of rice Jasmine85 (cm) in both crops of 2020-2021 Stillers (DAS) Treatments 15 35 55 100 Winter-Spring (A) T1 518a 549a 393a 350a T2 577ab 575ab 446a 402ab T3 729bc 683bc 533b 482b T4 766c 762c 538b 484b Summer-Autumn(B) T1 655ab 496 397 361a T2 661b 539 407 383ab T3 578a 553 413 393ab T4 654ab 549 428 405b www.turkjphysiotherrehabil.org 6788
Turkish Journal of Physiotherapy and Rehabilitation; 32(3) ISSN 2651-4451 | e-ISSN 2651-446X F(A) ** ** ** ** F(B) ** ns ns ** CVA(%) 21.8 17.8 16.5 16.9 CVB(%) 9.25 12.2 8.42 7.31 ns: insignificant differencesat the Pvalue= 0.05; **significant differencesat the Pvalue= 0.01 the Table 3 results performed sufficiently that Co-application treatments of lime, cow manure, Vian with NPK had higher number of tillers than the control treatment during growth stages of rice in both crops. The amendment treatments of lime, cow manure and Vian affected on the number of tillers during the rice growth of two crops. However, rice stillers were insignificant difference at 35 and 55 days after seeding in the Summer-Auturnm season (crop 2).Different kinds of cow manure, Vian and lime application were insignificantly affected on rice tillers during growth stages. Tillers developed rapidly at 15 DAS and reached the highest number of tillers at 35 DAS in the both crops. Maybe young stage of rice might be affected by soil fertility soil and rich rooting. Tillers of 55 and 100 DAS, which slightly reduced in both seasons were periods of rice to produce flowers. Rice tillers were already lessening from flowering stage until harvest. The highest number of available shoots reached at treatments of cow manure combined with NPK (NT4) and the lowest value of rice shoots were at control treatments (NT1) in the Winter-Springand Summer-Auturnm crop (Table 3).The positive ability of rice produces many stillers, which is an important role to help the good growth and raise the yield of rice (Duan et al., 2015). productivity and its components Table 3. The yield components of rice Jasmine85 in both crops of 2020-2021 Yield components Treatments 1000-grain Panicle length (cm) Fill grain (%) Fresh moisture(%) weight (gr) Winter-Spring (A) T1 20.8 92.0 30.5a 23.0 T2 21.2 91.2 33.2ab 24.9 T3 20.7 89.6 30.3a 26.5 T4 21.1 92.6 34.4b 25.5 Summer-Autumn(B) T1 21.4a 74.0a 29.7 22.2 T2 22.2ab 75.8ab 30.8 24.8 T3 22.3ab 78.8bc 29.2 24.1 T4 23.0b 80.0c 32.2 24.5 F(A) ns ns ** ns F(B) ** ** ns ns CVA(%) 4.24 2.25 7.20 11.0 CVB(%) 4,67 3,65 9.27 7.79 ns: insignificant differencesat the Pvalue= 0.05; **significant differencesat the Pvalue= 0.01 Panicle length Co-application of lime, cow manure, Vian and NPK was insignificant effects the fresh moisture of rice Jasmine85 in both crops. Panicle length of rice, which ranged from 20.7 to 23.0 cmand insignificant differences in the Winter- Spring season had significant differences in the Summer-Autumn season (Table 3). Fill grain The rate of fill grains depends on the organic fertilizer condition after one crop. Table 3 showed The percentage of fill grains increased significantly with application of cow manure application in crop 1.The fill grain of rice had no differences in crop 1 and varied in the crop 2. Fill grains of rice Jasmine85 ranged from 74.0% to 92.4% in both crops. Co-application of lime, cow manure, Vian and NPK (T4) was higher than that of other treatments. The number of fill grains is affected by rice varieties, fertilizers, and cultivation techniques (Nguyen, 2009). The prior stydy of Chuong et al., 2021 proved that application of cow manure, lime combined with NPK fertilizer increased the yield of peanuts in the next crop. www.turkjphysiotherrehabil.org 6789
Turkish Journal of Physiotherapy and Rehabilitation; 32(3) ISSN 2651-4451 | e-ISSN 2651-446X 1000-grain weight and Fresh moisture Results in Table 3 showed components of rice Jasmine85 that were the 1000-grain weight and fresh moisture had insignificant differences (Except 1000-grain weight of Winter-Spring season).The weight of 1000 grains is due to the genetic stability of the variety because the grain size is tightly controlled by the size of the hull. Therefore, grains cannot grow larger than the rice husk despite advantage conditions of soil fertily (Yoshida, 1981). Yield and quality Fresh yield of rice The rice yield was strongly affected by cow manure, lime and Vian amendment. The environmental conditions of Winter-Spring crop were more suitable for growth of rice than those in the Summer-Autumn crop. the maximum yield was 8.1 t/ha (T1) in the Winter-Spring crop and 5.83 t/ha (T4) in the wet season (Summer-Autumn crop) (Table 4). Fresh yield of rice valued from 6.79 t/ha to 8.10 t/ha (Winter-Spring crop) and 4.40 t/ha to 5.83 t/ha, respectively. The highest yield (8.10 t/ha) was the control and lowest value (6.79 t/ha) reached at the cow manure treatment in the crop 1. Application of organic fertilizer could reduce the yield because soil microorganisms take soil nutrients to increase its biomass (Hoqueet al., 2018; Chuong et al., 2021; Anaset al., 2020). the rice yield of Summer-Autumn crop, which was lower than the yield of Winter-Spring crop due to weather conditions was no application of lime, Vian and cow manure (Table 1). However, The rice yield of T2, T3 and T4 resulted higher than that of control treatment. The highest value reached 5.83 t/ha at the T4 treatment and lowest yield (4.40 t/ha) at the control; although the cow manure was not added in crop 2 but on the experimental background of crop. Many prior studies proved that application of organic fertilizers for crops increased the yield of plants after one crop (about three months) because organic fertilizers are completely digested by microorganisms and become useful nutrients for plants (Przemysław et al., 2020; Chuong et al., 2021, Hoque et al., 2018) Table 4. The yield and quality of rice Jasmine85 in both crops of 2020-2021 Treatments Characteristic Protein (%) Lipide (%) Yield (t/ha) Winter-Spring (A) T1 8.50 1.20c 8.10b T2 8.20 0.90b 7.22ab T3 8.10 1.00b 6.79a T4 8.50 0.70a 7.83ab Summer-Autumn(B) T1 7.00a 0.90a 4.40a T2 7.10ab 1.00a 4.84b T3 7.40ab 0.80a 5.55bc T4 7.50b 1.60b 5.83c F(A) ns ** ** F(B) ** ** ** CVA(%) 3.94 22.6 9.05 CVB(%) 4.50 31.7 12.3 ns: insignificant differencesat the Pvalue= 0.05; **significant differencesat the Pvalue= 0.01 Similar results in Table 4 were showed for the quality of rice Jasmine85 grains. The maximum protein and lipide values of Winter-Springcrop (8.50% and 1.20%, respectively) had at control treatment while the highest protein and lipide values (7.50% and 1.60%, respectively) were observed for T4 treatment in the Summer-Autumn season. There was insignificant difference among treatments for protein and lipid contents in rice grains in the crop 1. In Summer-Autumn crop, the values for protein and lipide ranged from 7.00 to 7.50% and 0.90 to 1.60%, respectively (Table 4). The highest results for protein and lipide of rice grains, which were reached at T4 treatment (7.50 and 1.6%), respectively obtained on the prior experimental background of the Winter-Spring crop.The prior studies has proved that application of cow manure with NPK fertilizers obtained higher concentrations of protein and lipide in rice Jasmine85 grains compared to the control treatment (Saha et al., 2014; Hossain et al. 2010; Myint et al., 2010) www.turkjphysiotherrehabil.org 6790
Turkish Journal of Physiotherapy and Rehabilitation; 32(3) ISSN 2651-4451 | e-ISSN 2651-446X IV. CONCLUSION Application of NPK fertilizer amended cow manure, Vian and lime for rice, which has insignificantly resulted positive effects on yield components, yield and nutrient values of rice in the Winter-Spring crop were positive impacts on growth, yield and quality of rice Jasmine85in the Summer-Autumn crop. The cow manure and lime residue of the Winter-Spring cropaffected on the the growth and yield of the Summer-Autumn crop. The amendment of 10.0 tons cow manure +85 kgN - 45 kgP2O5 – 45 kgK2O per ha had higher values of grain protein, grain lipide and rice yield than the control treament. The highest yield of rice jasmine85 reached 5.83 tons per haat the T4 treatment (cow manure amendment treatment of crop 1), which raised 24.5% compared to the T1 treatment in the Summer-Autumn crop. REFERENCES 1. AnasIqbalID, Liang He, Izhar Ali, SaifUllahID, Ahmad Khan, Aziz Khan, KashifAkhtar, Shangqin Wei, Quan Zhao, Jing Zhang, Ligeng Jiang. 2020. Manure combined with chemical fertilizer increases rice productivity by improving soil health, post-anthesis biomass yield, and nitrogen metabolism. PLoS ONE,15(10): e0238934. https://doi.org/10.1371/journal.pone.0238934 2. AtiyaSharminMitu, MdAsaduzzaman Khan and MdRaisUddinRashed. 2017. Effect of Fertilizer Manure and Lime on Growth and Yield of Boro Rice in Acidic Red Soi. Agri Res & Tech, 5(5): 1-5. 3. Bolan, N.S. and Duraisamy, V.P., 2015. Role of inorganic and organic soil amendments on immobilization and phytoavailability of heavy metals: a review involving specific case studies. Aust. J.Soil Res., 41:533-555. 4. Bray, R. H., and L. H. Kurtz. 1945. Determination of total, organic and available forms of phosphorus in soils. Soil Science 59:39–45. doi:10.1097/00010694-194501000-00006. 5. Chuong, N.V., Liem, P.V. and Tuan, H.T. 2021.Effects of Lime, Cow Manure Application with RhizobiumInoculation on Yield and Quality of the Peanut in the Grey Degraded Soil of Tri Ton town.Asian Journal of Soil Science and Plant Nutrition, 7(2): 1-10. 6. Chuong, N.V., Liem, P.V. and Tuan, H.V. 2021 .Effects of Lime, Cow Manure Application with Rhizobium Inoculation on Yield and Quality of the Peanut in the Grey Degraded Soil of Tri Ton town.Asian Journal of Soil Science and Plant Nutrition, 7(2): 1-10, doi:10.9734/AJSSPN/2021/v7i230106 7. De Ngoc Nguyen, TrucThanh Nguyen, Quy Ngoc Tran, Ben Macdonald, TuongPhuc Toc, Dung Van Tran and Qui Van Nguyen. 2017.Soil and Rice Responses to Phosphate Fertilizer in Two Contrasting Seasons on Acid Sulfate Soil. Communications In Soil Science And Plant Analysis,48(6): 615– 623;doi.org/10.1080/00103624.2016.1253719. 8. HoqueT.S., JahanI., IslamM.R. and Ahmed, A. 2018. Performance Of Different Organic Fertilizers In Improving Growth And Yield Of Boro Rice.SAARC J. Agri., 16(2): 153-166 9. KifayatullahKakar, Tran Dang Xuan, ZubairNoori, Shafiqullah Aryan and GulbuddinGulab. 2020. Effects of Organic and Inorganic Fertilizer Application on Growth, Yield, and Grain Quality of Rice. Agriculture, 10(544):1-11; doi:10.3390/agriculture10110544. 10. Kopittkea, P. M., Menziesa, N.W., Peng Wang, McKennaa, B.A., Lombic, E. 2019. Soil and the intensification of agriculture for global food security.Environment International, 132:1-8, 1050782. Doi.org/10.1016/j.envint.2019.105078 11. Mitu, A.S., Khan, Md. A. and Rashed, Md. R. U. 2017. Effect of Fertilizer Manure and Lime on Growth and Yield of Boro Rice in Acidic Red Soil.Agri Res & Tech, 5(5):1-6. 12. Nguyen, N. D. 2009. Rice textbook. Ho Chi Minh City, Vietnam: National University Publishing House. 13. Ning, C.C., Gao, P.D., Wang, B.Q., Lin, W.P., Jiang, N.H., Cai, K.H. 2017. Impacts of chemical fertilizer reduction and organic amendments supplementation on soil nutrient, enzyme activity and heavy metal content.Journal of Integrative Agriculture, 16(8): 1819–1831.Doi: 10.1016/S2095- 3119(16)61476-4. 14. PrzemysławBarłóg, LukášHlisnikovskýand EvaKunzová. 2020. Effect of Digestate on Soil Organic Carbon and Plant-Available Nutrient Content Compared to Cattle Slurry and Mineral Fertilization. Agronomy,10(379):1-16; doi:10.3390/agronomy10030379 15. Thuc, L.V., Jun-Ichi, S., Khuong, N.Q. Susan, O., Huu, T.N., Lang, N.T.T., and Nhan, P.P. 2021. Effects of Spraying Gibberellic Acid Doses on Growth, Yield and Oil Content in Black Sesame (Sesamumindicum L.).Asian J. Crop Sci., 13: 1-8. DOI: 10.3923/ajcs.2021.1.8. 16. Wayne Sabbe, (1980).Standardizing Soil Testing Methods. Journal of Association of Official Analytical Chemists, 63(4):763–765, doi.org/10.1093/jaoac/63.4.763. 17. Yoshida, S. (1981).Fundamentals of rice crop science. IRRI. Philippines. 18. Yoshida, S. (1985), Fundamental of rice crop science, International Rice Research Institute, Philippines. www.turkjphysiotherrehabil.org 6791
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