SMART MATERIALS FOR NEXT GENERATION SCIENCE TM
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SMART MATERIALS FOR NEXT GENERATION SCIENCE TM
Contents
FluoroSurfactants p. 02
High Throughput Biology p. 04
Chip-Free Engineered Droplet Generation p. 05
Superparamagnetic Gel Beads p. 07
Barcoded Gel Beads p. 08
Universal Gel Beads p. 09
Microbe Capture & Detection p. 10
Ordering Information p. 12
1
FluoroSurfactants
Smarter surfactants. Stronger Science.
aqueous
n Your findings can only go as far as your tools allow. For
droplet
lsio
biologists working with droplet microfluidics, it means
interior
emu
trusting your rare materials and key experiments to the
amphiphiles
quality of your FluoroSurfactants. Low-performing chemicals
ulsio n
stabiliized cost time. And maybe more.
interface
em
er
s TOOLS TO
e
rev
TAKE YOU
oil
FURTHER.
008-Fluorosurfactant stabilizes custom and commercially available reaction mixes
Hot Start High Fidelity RT-qPCR
PCR Master Mix PCR Master Mix PCR Master Mix Master Mix
Pre Thermal
Cycling
Post Thermal
Cycling
Droplet PCR
+ Control
100 Base Ladder
RAN Biotech develops non-ionic
THE HIGHEST STANDARDS
FluoroSurfactants specifically for the most
rigorous researchers. We’ve worked for
> Chemical & biological compatibility
years with our customers to achieve
> High purity biocompatibility, reproducibility and
> Reproducibility stability that meets the highest standards.
> Custom chemical modifications
BECAUSE SUCCESS IN YOUR
> Custom product packaging LAB BEGINS IN OURS.
> Experienced technical & scientific
support Our FluoroSurfactants have been
optimized for droplet microfluidics and
for emulsion-based molecular biology.
2Products | FluoroSurfactants
Neat (un-dissolved) 008-FluoroSurfactant
Product Catalogue Number
Neat (un-dissolved) 008-FluoroSurfactant
1g 008-FluoroSurfactant-1G
Product Catalogue Number
5g 008-FluoroSurfactant-5G
1g 008-FluoroSurfactant-1G
10g 008-FluoroSurfactant-10G
5g 008-FluoroSurfactant-5G
50g 008-FluoroSurfactant-50G
10g 008-FluoroSurfactant-10G
50g 100g
008-FluoroSurfactant-50G 008-FluoroSurfactant-100G
100g 0.5kg
008-FluoroSurfactant-100G 008-FluoroSurfactant-500G
0.5kg 1kg
008-FluoroSurfactant-500G 008-FluoroSurfactant-1000G
1kg 008-FluoroSurfactant-1000G
008-FluoroSurfactant dissolved in common fluorinated oil
008-FluoroSurfactant dissolved in common fluorinated oil Product Catalogue Number
Product Catalogue Number
50 g of 2 weight % 008-FluoroSurfactant in HFE7500 008-FluoroSurfactant-2wtH-50G
50 g of 2 weight % 008-FluoroSurfactant in HFE7500 008-FluoroSurfactant-2wtH-50G
50 g of 2 weight % 008-FluoroSurfactant in FC40 008-FluoroSurfactant-2wtF-50G
50 g of 2 weight % 008-FluoroSurfactant in FC40 008-FluoroSurfactant-2wtF-50G
500 g of 2 weight % 008-FluoroSurfactant in HFE7500 008-FluoroSurfactant-2wtH-500G
500 g of 2 weight % 008-FluoroSurfactant in HFE7500 008-FluoroSurfactant-2wtH-500G
500 g of 2 weight % 008-FluoroSurfactant in FC40 008-FluoroSurfactant-2wtF-500G
500 g of 2 weight % 008-FluoroSurfactant in FC40 008-FluoroSurfactant-2wtF-500G
20 g of 5 weight % 008-FluoroSurfactant in HFE7500 008-FluoroSurfactant-5wtH-20G
20 g of 5 weight % 008-FluoroSurfactant in HFE7500 008-FluoroSurfactant-5wtH-20G
20 g of 5 weight % 008-FluoroSurfactant in FC40 008-FluoroSurfactant-5wtF-20G
20 g of 5 weight % 008-FluoroSurfactant in FC40 008-FluoroSurfactant-5wtF-20G
200 g of 5 weight % 008-FluoroSurfactant in HFE7500 008-FluoroSurfactant-5wtH-200G
200 g of 5 weight % 008-FluoroSurfactant in HFE7500 008-FluoroSurfactant-5wtH-200G
200 g of 5 weight % 008-FluoroSurfactant in FC40
200 g of 5 weight % 008-FluoroSurfactant in FC40
008-FluoroSurfactant-5wtF-200G
008-FluoroSurfactant-5wtF-200G
droplet interfaces illuminated by FluoreFS
Starter Kits
Starter Kits
Product Product
Catalogue Number Catalogue Number
008-FS in HFE7500 (10g) at 5wt% 008-FS in HFE7500 (10g) at 5wt%
008-FS in FC40 (10g) at 5wt% 008-FS in FC40 (10g) at 5wt%
StarterKit#1 FS-Kit#1
StarterKit#1
Neat HFE7500, 50g Neat HFE7500, 50g
Neat FC40, 50g Neat FC40, 50g
Neat 008-FS (1g)
Neat 008-FS (1g)
Neat HFE7500, 60g StarterKit#2
Neat HFE7500, 60g FS-Kit#2
StarterKit#2
Neat FC40, 60g
Neat FC40, 60g
Functionalized FluoroSurfactants
Functionalized FluoroSurfactants
Functionality Catalogue Number
Functionality Catalogue Number
Biotin FS-Biotin
Biotin FS-Biotin
BenzylGuanine FS-BG
BenzylGuanine FS-BG
Sugar FS-Sug
Fluorescein FluoreFSSugar FS-Sug
Rhodamine Fluorescein
FS-Rhodamine FluoreFS
Rhodamine FS-Rhodamine
3Simplifying high-throughput biology
Droplets are a powerful tool for biology allowing for the parallelization of up to millions of
samples or reactions.
Throughput increases significantly when using droplets
NEXT STANDARD
PLATE
1536
1
384
1
96
1
reactions / plate
reactions / well
LEVEL
0.012mL
0.025mL
0.2mL
1536 384 96 same wells but filled
BIOLOGY. vs
well well well with 50µm droplets
DROPLETS 183,000 382,000 3,000,000 reactions / well
281,088,000 146,688,000 288,000,000 reactions / plate
Mature tools and methods exist for the partitioning of samples into droplets, and RAN Biotech’s smart
materials support all of them. Whether you are using microfluidic chips or high throughput particle-
based approaches, our materials will ensure that your samples are efficiently partitioned and that they
remain stable throughout the entire workflow.
Common droplet generation workflows for cell and molecular biology
polydisperse
vortex/shake
droplets
aqueous
oil
droplet
microfluidics
setup
monodisperse
aqueous droplets
oil
e tip
pipett
aqueous monodisperse
gel bead droplets
oil PTE
satellite droplets
4Applications | Particle Templated Emulsification
RAN Biotech is introducting the first commercial kit for Particle Templated
Emulsification. This kit includes the reagents you’ll need to generate an
emulsion within minutes. No need for pumps or chips, even for monodisperse
droplets, just find a pipette or vortex, and you’ll be well on your way.
ulsion
m
e
e tip
SAY HELLO
pipett
Particle
TO THE Templated reagents
TM
NOCHIPDROP Emulsification gel
bead
KIT. fluoro-
cell reagent surfactant
layer
The NoChipDrop kit can be used to emulsify simple alginate
reactions such as PCR as well as to co-encapsulate cells
with barcoded beads for single-cell genomics workflows
crosslink & gel bead
and trapping of cells in 3D hydrogels. remove oil
PTE-based 3D hydrogel production
trapped
cell alginate
growth hydrogel
media
Iterative fluid templation workflows
1. PTE
cell trapped
w/o
in alginate
interface
w/o gel bead
droplets
A549 cell
prepolymer 2. polymerize/crosslink PTE
gel bead 3. remove oil alginate
shell
reagent/media
phase
alginate acrylamide agarose
shell shell shell
HERE’S TO WORKING SMARTER, NOT HARDER.
5| Chip-Free Engineered
Products | NoChipDrop Kit
Products Droplet Generation
ADVANTAGES OF THE NOCHIPDROPTM KIT
> Generate an emulsion of monodisperse droplets in 90 seconds.
> Use standard lab equiptment you already have (pipette or
vortex) and our surfactants and gel beads.
> Our gold-standard surfactants support a variety of enzymatic
formulations for applications such as PCR, cDNA synthesis, and
single cell encapsulation.
> Take droplet generation to the next level: generate multiple
samples at once with a multi-channel pipette still in only 90
seconds.
> Customize beads to fit your needs. Explore our highly
customizable beads on page 8.
> Recover your aqueous phase with ease.
Chip-Free Engineered Droplet Generation Kit (NoChipDropTM kit)
Product Catalogue Number
Emulsion Oil – 500μL
Aqueous Recovery Oil - 125μL NoChipDrop-Kit1
Gel Bead Slurry - 350μL (ca. 1.27M 58um beads)
Emulsion Oil – 2.2mL
Aqueous Recovery Oil – 0.55mL NoChipDrop-Kit2
Gel Bead Slurry – 1.4mL (ca. 5.08M 58um beads)
6Hydrogel Beads
highly
porous
customizable
OUR
reliably
BEADS compressible
monodisperse
ARE...
mechanically
stable
Our highly customizable beads were developed and
pliable optimized by our team of scientists to give you flexibility in
your workflow. Choose from a variety of functionalities, sizes,
sequencing adaptors and more.
Use our Functionalized Gel Beads (FunGelBeadTM) in proven
widely technologies such as DropSeq, inDrops, Single-Cell RNA
applicable sequencing, or the next game-changer brewing in your mind.
dissolvable
RAN Biotech’s superparamagnetic gel beads
are fully functionalizable, compliant and highly
reproducible magnetizable.
superpara-
magnetic
7Applications | Barcoded Gel Beads
RAN Biotech’s barcoded gel beads are highly functionalized, mechanically
stable and compressible. Our beads perform well when using standard
microfluidic approaches as well as with PTE-based methods.
BARCODED
GEL
BEADS.
RAN Biotech’s polyacrylamide and superparamagnetic polyacrylamide
beads with inDrops sequences and barcodes
Photocleavable T7 Promoter PE1 Barcode Barcode UMI TTTTTTTTTTTTTTTTTTT barcoded
linker capture cligo
90
bead diameter (µm)
T
TTATAA
A 80
70
barcoded polyacrylamide 60
hydrogel
50
70
bead diameter (µm)
60
ATATT 50
AAT
barcoded superparamagnetic 40
polyacrylamide hydrogel
FISH and cleavage QCs to highly compressible 30
confirm beads bear barcodes
FUNGELBEADTM
> Highly customizable bead sizing
> Choose from a variety of physical properties
microns
(superparamagnetic, dissolvable, fluorescent)
> Chip-based and chip-free compatible
> Modifiable functionalization to help you capture
what you’re looking for
8Products | Gel Beads
RAN Biotech’s proprietary UniversalBeadTM Technology lets you functionalize hydrogel beads
in your own lab with your molecule of choice via commonly used chemical modifications.
Post-polymerization bead functionalization
with a photocleavable DNA oligo via an
RAN Biotech’s UniversalBeads azide modification.
Gel Bead Modification
present exceedingly high numbers
of functional groups across the
NH 2
DBC
inner and outer bead surfaces.
NH 2
DB
NHS ester
DBCO
O
CO
DBC
NH 5’ phosphate NHS-FAM
2
O
3’ phosphate
NH Azide - iSpPC - DNA (67 nt) . . . .
2 . . . . DNA - FAM
O be
DBC ad
s in
E
bu
)
DBCO
ts
PT
ffe
le
r
p
ro
Azide-FAM
(d
DBC
O azide bead
DB
CO
OH droplet
OH
λ365nm
for 30s
OH
amine
O
H
bead
1 micron λ365nm
for 60s
SEM of gel bead
droplet
Gel Beads
Try functional gel Product Name
beads in your Non-functionalized gel beads DummyBeads
NoChipDropTM kit
inDrops Photocleavable Barcode Beads PCBarcodeBeadsTM
Functionalized Gel Beads, including Barcoding,
Dissolvable and Superparamagnetic Gel Beads - FunGelBeadsTM
Custom Ordering
Chemically functionalized beads that react with
NHS/azide/amine/hydroxyl modified molecules UniversalBeadsTM
>Amine>DBCO>Hydroxy>Custom
9Microbe Capture & Detection
RAN Biotechnologies’ patented microbe capture materials mimic the receptors that microbes use to attach
to mammalian cells, and can be used to capture microbes for detection, isolation and and even identification.
RAN Biotech’s affinity capture resins consists of hyperbranched
chains of receptors attached to a functional scaffold.
Receptor mediated
prote o lycan
receptors
concentration of microbes
g
at a cell surface.
hyperbranched
linker
resin sa
m ple
scaffold
adsorption (%)
Microbes have a strong affinity for the receptors while the
scaffolding material can be actvated for the capture of qe
nucleic acids or even magnetized.
Mississippi River Isolates - From detection to metagenomics CFU/mL
ri
a mv e r passive
s
ple S1 S2 S3 S4 S5 pellets from river pellet
detection samples formation
assays
S1 S2 S3 S4 S5
kb
time
4 S1
Illumina
S2
2 S3
fluorescence
Nextera XT
S4
isolate 1.25 metagenomic
gDNA
0.8 prep sequencing
0.5
libraries
0.3
0.2 gDNA isolated from inoculum
0.1 assay pellets
0.1 0.2 0.3 0.4 0.6 1 3 10.4
length (kb)
Direct cultivation of microbes
Syst
Class em 1 from detection assay pellets
n
50 % of ai
Sp
m
Do
ec
Max
45
ie
s
ily
80
r4
MiSeq 40
analysis
Fam
Rive
35
30 60
Genus
Q score
25
3
20 0 20 40 60 80 100 120 140 160
River
15
Cycle
Or
de
10 r2
ve
r
Phy
5 lum Ri
0 River 1
We have optimized a process that allows for molecular biology
and sequencing-based identification of the captured microbes GET MORE FROM
from the detection assays. YOUR FINDINGS.
10Products | RMD
When microbial growth represents a risk to safety, efficiency, or functionality, then detecting and
measuring their presence is essential. But culture-plate methods take days, and newer rapid microbe
detection methods mean a capital investment and a higher cost per test.
Not with RMD. Our patented approach detects microbes accurately,
affordably and within hours, all with our easy-to-learn technology that fits
in the palm of your hand.
Our RMD
assay is as
easy as Mix your sample Confirm microbial presence using our
1, 2, 3: and RAN Biotech Incubate handheld ViewerDevice or simply by
resins. looking with the naked eye.
RMD Products
Product Catalogue Number
Aqueous suspension of proprietary RMD1/2 resins designed to
universally capture microbes. RMD1/2-GM
Custom amounts depending on test sample size
Patent–pending ViewerDevice assists in collecting and analyzing ViewerDevice
images of the visible RMD Pellet and mobile phone (optional)
Library construction and sequencing LibraryConst/Seq
11WE’RE HERE
FOR YOU.
Efficiency and immediate-order info@ranbiotechnologies.com
processing are at the core of
our customer support.
+1 833 726 2661
To order, please submit a quote
request by email, phone, or visit
www.ranbiotechnologies.com
our website.
For payment, we accept Purchase Orders (Net30Days) and all major
credit cards (Visa, Mastercard, American Express, and Discover).
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