Product Catalogue 2021 - Life Science for a Better Life - Solis BioDyne
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Product Catalogue 2021 Life Science for a Better Life
Dear Partners, About us
I am pleased to announce that Solis BioDyne has turned an Solis BioDyne manufactures high-quality molecular
important page in our history. Thanks to 25-years of dedicated biology reagents since 1995. The company and
work, our valued partners and the strictest dedication to state- main production is based in Tartu, Estonia (EU),
of-the-art science, we are now focused on significant expansion. an international academic town with a growing
We urge you to join us! biotechnology sector. Our youthful and growing team
is dedicated to providing:
We believe that success lies in delivering increased value to our
customers, which is why quality has always been our number • state-of-the-art products
one priority. We are determinedly investing in developing new • sustainable solutions
products, improving technical support and meeting even higher • personal service
quality standards.
Our product line-up includes DNA polymerases,
During the year of Covid, we have established several new key master mixes for PCR/qPCR, reverse transcriptases
partnerships, from all over the world. Our dedicated support and other reagents – all safe to handle at room
team has stepped up despite the challenges, and we have temperature thanks to our patented polypeptide
managed to deliver personalized and successful results for stabilization technology. Our technology has enabled
our customers. our customers to completely eliminate the need for
cold-chain transport of enzymes that are traditionally
In 2021, we will continue to focus on these key areas, while considered extremely sensitive to ambient tem-
expanding production capacity through innovation and peratures. For 25-years, all of our products have
improvements. We thank you for your continued support. been shipped without ice, to our satisfied customers
around the globe.
Sincerely,
By choosing Solis BioDyne products, you choose
Oliver Ploomipuu sustainability, without increasing costs or sacrificing
Chief Executive Officer product quality.
PRODuCt CatalOguE 2021 1
Table of Contents
Polypeptide Stabilization technology: Stability tag 2
Quality Control & ISO Standards 5
Product Format and Packaging 5
Endpoint PCR Enzymes and Master Mixes: Product Selection guide 6
Hot-start PCR Mixes
Standard PCR Mixes
PCR Enzymes
qPCR Mixes: Product Selection guides 16
Dye-based qPCR: Mix Compatibility table 17
Dye-based qPCR Mixes
Probe-based qPCR: Mix Compatibility table 23
Probe-based qPCR Mixes
First-strand cDNA Synthesis 29
FIREScript® Reverse transcriptase and cDNa Synthesis Kits and Mixes
One-step Rt-qPCR Kits: Product Selection guide 31
NEW! Dye-based Rt-qPCR Kit
Probe-based Rt-qPCR Kits
additional Enzymes and Reagents 36
Enzymes for MalDI-tOF
Nucleotides: dNtP Mix and Set
DNA Ladders: 100 bp DNA Ladder, 1 kb DNA Ladder
DNA Loading Dye Buffers
Other Reagents: gC-rich Enhancer, 25 mM MgCl2, PCR grade Water
Ordering 40
International Presence & Distributors 42
Product List 44
Teaduspargi 9, 50411 Tartu, Estonia VAT No: EE100587614
T: +372 7409 960 Reg. No: 10242922
F: +372 7402 079
E: info@solisbiodyne.com Bank details: Swedbank AS
Skype: solis.biodyne IBaN code: EE692200221005142234, SWIFt/BIC.: HaBaEE2X
W: solisbiodyne.com Bank address: Liivalaia 8, 15040 Tallinn, Estonia
2
Polypeptide Stabilization
Stability tag
Technology: Stability TAG
3D MODEL OF FIREPOL® DNA POLYMERASE Convenient reaction set-up
INCLUDING STABILITY TAG
Our room temperature stable enzymes
allow ice-free reaction set-up.
• Saves valuable bench space
• Convenient for high-throughput workflows
• No harm is done if you forget your enzymes out of fridge
for weeks
REACTION REACTION SET-UP
SET-UP ON ICE AT ROOM TEMPERATURE
Taq DNA Polymerase Stability TAG
Safe storage
“
Our reagents remain fully active even after a power outage has In May 2019 I got a free sample of HOT
damaged everything else in your freezer, after someone has FIREPol® EvaGreen® Supermix at the
forgotten the reagents on the table overnight, or if there have Labvolution in Hannover. While unpacking
been delays in the customs during shipment. Routine storage at my bag it slipped into the corner of a drawer
-20°C is required to ensure maximum shelf-life. and was unfortunately forgotten. So it
stayed in the drawer in darkness at room
temperature. I found it again in January
2020! So after 7 months without cooling. I
gave it to my undergraduate students for a
No need to pay DRY ICE
IN REGULAR small lab project. They did a comparison of
for shipping the ice INSULATED BOX
qPCR Mastermixes with our Illumina ECO
ACTUAL on a Lambda DNA Template. The students
PRODUCT SIZE found your 5xHotFirePol Supermix was
working best of all! Even after that time of
faulty storage. The results in sensitivity and
”
reproducibility were very satisfying.
Ice-free shipping
Our Stability tag technology means we can ship your order
DIPL-ING CHRISTINA STEFFEN
without dry ice and large insulation boxes, which is better both
Wissenschaftliche Mitarbeiterin,
for the environment and your budget:
Molekularbiologie
• less material used for packaging
• lower package weight
• less fuel used for transportation
• lower shipping charges for you
• no additional regulations for shipping and receiving the goods
• accessible for all labs
PRODuCt CatalOguE 2021 3
STABILITY OF HOT FIREPOL® DNA POLYMERASE
Stability TAG ensures product
Stability tag
HOt FIREPol® Probe universal qPCR Mix shows no loss of activity after
stability at room temperature* for incubation at room temperature for 1 month (upper graph) compared
to storing at –20⁰C (lower graph).
30 days
All enzymes produced at Solis BioDyne, including DNA
polymerases and reverse transcriptases, as well as other proteins
(i.e. RNase inhibitor, uracil-N-glucosylase), are exceptionally
stable at room temperature due to a proprietary genetic
modification in the polypeptide structure - Stability TAG (EU
Patent EP2501716, Korea Patent No 10-1773636 and uS
Patent No 9,321,999).
All our products can withstand 1 month at room temperature
without detectable change in the performance of the product,
which enables shipping our products without ice. the exceptional
product stability is furthermore supported by our unique buffer
composition.
Stability tag enhances also long-term stability of our enzymes
stored at -20°C which is the recommended storage temperature
of all our products upon arrival, to ensure maximum shelf-life
HOt FIREPol® Probe HOt FIREPol®
universal qPCR Mix Probe Universal
* Room temperature is 15-25°C according to "guidelines for the room temperature qPCR Mix -20°C
Storage of Essential Medicines and Other Health Commodities",
World Health Organization (2003)
1E01 STABILITY OF SOLISFAST® DNA POLYMERASE
SolisFAST® Probe qPCR Mix (Purple) was used in 4-plex qPCR
amplification with 3 tenfold serial dilutions of human gDNa (from
1E00
2 ng/µl to 0.02 ng/µl). Reactions were run on applied BioSystems™
QuantStudio™ 6 Flex cycler using Purple dye for normalization.
SolisFAST® Probe qPCR Mix (Purple) shows no loss of activity after
0.177046
0.158365
0.138548 incubation at room temperature for 1 month (upper graph) compared
0.1
to storing at –20⁰C (lower graph).
0.04824
FaM JUN ABY VIC
0.01
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44
Cycle
1E01
1E00
0.177046
0.158365
0.138548
0.1
0.04824
0.01
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44
Cycle
4
STABILITY OF SOLISCRIPT® 1-STEP COV KIT
Stability tag
SOLIScript® 1-step CoV Kit contains 3 different polypeptides with
Stability tag – SOlIScript® Rt, Ribogrip™ RNase inhibitor and
HOt FIREPol® DNa Polymerase. the kit was used in a 4-plex qPCR
experiment with 4 tenfold serial dilutions of human total RNa (from 4
ng/µl to 4 pg/µl). Reactions were run on Bio-Rad CFX96 qPCR platform.
SOLIScript® 1-step CoV Kit shows no loss of activity after incubation
at room temperature for 1 month (upper graph) compared to storing
at –20⁰C (lower graph).
FaM ROX VIC Cy5
Amplification Plot Melt Curve Plot STABILITY OF FIRESCRIPT®
REVERSE TRANSCRIPTASE
1E01
3.0 Human total RNa (10 ng) was converted
2.5
into cDNA using oligo-dT primers and
2.0
FIREScript Rt (10 u/µl). Real-time PCR
was performed using ß2M primers and HOt
ΔRn
1E00
1.5
1.0
FIREPol® Evagreen® qPCR Mix Plus. there is
no change in enzyme activity after incubation
0.5
of FIREScript® Rt at room temperature for 1
0,1
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44
65.0 70.0 75.0 80.0 85.0 90.0 95.0 month (upper graphs) compared to storing
Cycle
at –20⁰C (lower graphs).
1E01
3.0
2.5
2.0
ΔRn
1E00
1.5
1.0
0.5
0,1
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44
65.0 70.0 75.0 80.0 85.0 90.0 95.0
Cycle
-20°C 22°C / 1 week 22°C / 1 month Human total RNa (10 ng, 1 ng and 0.1 ng) was
converted into cDNA using oligo-dT primers
and FIREScript® Rt (10 u/µl) that had been
stored at –20⁰C and at room temperature
(22°C) for 1 week and for 1 month. PCR
was performed using ß2M primers and HOt
FIREPol® Blend Master Mix.
10 1 0,1 10 1 0,1 10 1 0,1
Total RNA (ng) Total RNA (ng) Total RNA (ng)
PRODuCt CatalOguE 2021 5
Quality Control ISO Certification
Quality Control & Product Format
Solis BioDyne's production and product development Quality has always been the core value of our work. To ensure
takes place in a state-of-the-art laboratory, under the we match the high-quality requirements of our partners in the
supervision of experienced scientists and technicians. research and diagnostic sector, we implemented the ISO 13485
standard in 2018 to complement the ISO 9001 certificate we
To ensure the highest quality and lot-to-lot consistency,
acquired in 2007. Our ISO certificates ensure
strict quality procedures are followed and documented
at all stages of production. Certificates of analysis (Coa) • High-quality products
are available for each lot upon request.
• Lot-to-lot consistency
• Supply chain security and traceability
• Manufacturing process consistency
Solis BioDyne Quality
Management System is
certified to ISO 13485:2016
and ISO 9001:2015
standards.
Product Format and Packaging
5x concentration COMPARISON OF OUR 5X MASTER MIX
TO A STANDARD 2X MASTER MIX
all Solis BioDyne PCR and qPCR mixes are produced Reactions (20 µl
Vial size
in 5x-concentrated solutions to improve stability. the final volume)
higher buffer concentration also leaves 2.5 times more
2x Standard Master Mix 1 ml 100 rxn
room for your template and primers compared with
2x mixes. this is highly advantageous when working 5x Solis BioDyne
1 ml 250 rxn
Master Mix
with low-concentration DNA samples or performing
multiplex assays.
Get 2.5x more reactions
Light-protective packaging done with same volume of
PCR/qPCR Mix
Fluorescent dyes Evagreen®, Solisgreen®, and passive
reference dyes (ROX, Purple) are sensitive to degradation
by light. Solis BioDyne qPCR mixes that contain these
dyes are supplied in special dark vials to minimize light
Update in Product Format
exposure during transportation and reaction set-up.
In 2020, we have implemented several changes in the product
presentation, such as a new tube design, updated Data Sheet
design and content, tube labelling, etc. For example, you will
notice that all of our PCR and qPCR master mixes will no
longer have “5x” (reference to product concentration) in front
of the product name, in order to align with market standards
and reduce mistakes in ordering.
In 2021, we will continue updating product technical
documentation, as well as outer packaging and labelling. To
avoid waste of materials already in stock, we will implement the
updated designs gradually. This means that for our customers,
you may receive some products still in the old packaging, as
they are phased out of production.
6
Product Selection Guide:
Endpont PCR: Product Selection guide
Endpoint PCR Enzymes and Master Mixes
Ready Ampli- gC-rich Multi-
Hot dUTP+ Fidelity Resulting
To fication Speed perfor- plex Page(s)
Start uNg vs. Taq ends
Load Rangea mance PCR
NEW! SolisFAST®
Master Mix • 1x 5 kb 3'A *** * *** 7-8
NEW! SolisFAST®
Master Mix • • 1x 5 kb 3'A *** * *** 7-8
Ready To Load
NEW! SolisFAST®
Master Mix with • • 1x 5 kb 3'A *** * *** 7-8
UNG
NEW! SolisFAST®
Master Mix with • • • 1x 5 kb 3'A *** * *** 7-8
UNG Ready To Load
HOT FIREPol®
Multiplex Mix • 1x 5 kb 3'A * * *** 9
HOT FIREPol®
Multiplex Mix • • 1x 5 kb 3'A * * *** 9
Ready To Load
HOT FIREPol® 3'a/
Blend Master Mix • 5x 5 kb
Blunt
* * * 10-11
HOT FIREPol®
3'a/
Blend Master Mix • • 5x 5 kb
Blunt
* * * 10-11
Ready To Load
HOT FIREPol®
GC Master Mix • 1x 5 kb 3'A * *** * 12
FIREPol® Master Mix 1x 5 kb 3'A * * * 13
FIREPol® Master Mix
Ready To Load • 1x 5 kb 3'A * * * 13
HOT FIREPol® DNA
Polymerase • 1x 5 kb 3'A * ** ** 14
FIREPol® DNA
1x 5 kb 3'A * ** ** 15
Polymerase
a
Enables amplification of up to 5 kb fragments from low complexity DNa templates (e.g. cDNa,
lambda, plasmid DNA), and up to 3 kb from genomic DNA (human, animal, plant).
PRODuCt CatalOguE 2021 7
RESULTS IN 20 MINUTES
SolisFAST PCR ®
Hot-start PCR Mixes
SolisFAST® Master Mixes are suitable for ‘slow’ and ‘fast’
PCR cyclers. HIRA gene fragment (515 bp) from human
Master Mixes gDNa was amplified using SolisFaSt® Master Mix (lane
1-2) and Master Mix with uNg (lane 3-4). Fast cycling
settings (initial activation 1 min at 98°C; denaturation
5 sec at 98°C, annealing/extension 20 sec at 60°C, 25
Description cycles) were used on Biometra T1 Thermocycler (lane 1
and 3) and Eppendorf® Mastercycler® X50s (lane 2 and 4).
a ready-to-use 5x-concentrated solution for fast and ultra-fast singleplex
and multiplex endpoint PCR assays. It contains a novel SolisFaSt® DNA
Polymerase with fast hot-start (30 sec to 3 min) and 2-4 times faster Cycler’s PCR
extension rates (15-30 sec/kb) compared to the wild-type taq DNa PCR cycler ramp run time
rate (min)
polymerase (60 sec/kb), HOt FIREPol® and FIREPol® DNA polymerases.
the mix allows amplification of up to 5 kb DNa templates and has Biometra T1 =
4°C/sec 26
two versions - regular and Ready to load mix which includes loading ‘slow’ machine
dyes for direct loading to gel. additionally, mixes with dutP and uNg
Eppendorf X50s
are available to prevent carry-over contamination. Experiments with = ‘fast’ machine
10°C/sec 20
SolisFAST® Master Mix with dutPs and uNg have also shown a more
robust performance with difficult sample materials (i.e soil).
bp M 1 2 3 4
Benefits
• PCR results in 20 minutes
• fast hot-start (30 sec-3 min)
• fast amplification (15-30 sec/kb)
• sensitive detection of up to 18 targets per reaction
• suitable for templates up to 5 kb
• reliable results in Sanger sequencing applications
• Ready To Load mix available for direct loading to gel
• UNG-mix available to prevent carry-over contamination
• reaction set-up and shipment without ice
Reference:
“
I first attempted to amplify an insect DNA gene with HOT
FIREPol Blend Master Mix in soil samples containing many
inhibitors. The few amplicons obtained were non-specific. Tip!
I then tested the Solis FAST Master Mix UNG on the same
Reduce reagent cost and
samples and was finally able to get better yield and specific
”
reaction set-up time by
amplifications of my target gene.
detecting multiple targets
in a single reaction.
MELLE ELIANE LOUISANNA
UMR ECOlogie des FOrêts de Guyane (ECOFOG)
bp M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 EXCELLENT SINGLEPLEX AND
MULTIPLEX PCR RESULTS
122 bp 177 bp 273 bp 389 bp 515 bp 642 bp 801 bp 988 bp 1199 bp
145 bp 216 bp 339 bp 438 bp 584 bp 722 bp 908 bp 1080 bp 1340 bp Eighteen fragments of human gDNA (ranging from 122
to 1340 bp) were amplified in singleplex (lane 1-18) and
multiplex (lane 19) PCR. amplifications were performed
as a single run on Eppendorf® Mastercycler® X50s using
3-step cycling program optimized for multiplex assays
(initial activation 2 min at 98°C; denaturation 10 sec at
98°C, annealing 10 sec at 60°C, extension 30 sec at
72°C (30 cycles).
8
Send your sample request to orders@solisbiodyne.com
Hot-start PCR Mixes
PRODuCt CAT. NO. RXN/20 µl SIZE in ml
24-01-0000S (free sample) 50 0.2
24-01-00001 250 1
SolisFAST Master Mix
®
24-01-00001-5 5 x 250 5x1
24-01-00020 5000 20
24-02-0000S (free sample) 50 0.2
SolisFAST® Master Mix 24-02-00001 250 1
Ready To Load 24-02-00001-5 5 x 250 5x1
24-02-00020 5000 20
24-21-0000S (free sample) 50 0.2
24-21-00001 250 1
SolisFAST Master Mix with uNg
®
24-21-00001-5 5 x 250 5x1
24-21-00020 5000 20
24-22-0000S (free sample) 50 0.2
SolisFAST® Master Mix with uNg 24-22-00001 250 1
Ready To Load 24-22-00001-5 5 x 250 5x1
24-22-00020 5000 20
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.comPRODuCt CatalOguE 2021 9
HOT FIREPol® MultiPlex Mix &
Hot-start PCR Mixes
MultiPlex Mix
Ready To Load
SENSITIVE AND SPECIFIC RESULTS
Description Different genes from human gDNa were amplified in
multiplex reactions using HOt FIREPol® MultiPlex Mix.
a ready-to-use 5x-concentrated solution for singleplex and multiplex Amplicons ranging from 122 bp to 1340 bp show similar
yield and high specificity with simultaneous amplification
endpoint PCR assays. It contains a hot-start taq DNa polymerase HOt in 4-, 8-, 14, and 18-plex PCR assays.
FIREPol® and allows amplification of up to 5 kb DNa templates. the
mix has two versions - regular and Ready to load mix which includes
M 4-plex 8-plex 14-plex 18-plex M
loading dyes for direct loading to gel.
Benefits
• sensitive detection of up to 18 targets per reaction
• suitable for templates up to 5 kb
• increased sensitivity and yield
• reduced primer dimer formation
• Ready To Load mix available
• reaction set-up and shipment without ice
SIIA UUS PILT
Researchers already trust MultiPlex Mix
Reference:
“
Convinced with the performance and quality of the product
in multiple applications: robust enzyme activity and
reproducible results in single and highly multiplexed PCRs.
”
A ‘‘must-have’’ in the laboratory. Did you know?
Products specifically
developed for multiplex
DR. SERGEY YAKUSHEV
assays contain sufficient
Head of the laboratory
amount of reaction
Microsynth, Switzerland
components for accurate
amplification of all
targets.
Selected publications:
• Bangratz, M. et al., PloS One. (2020)
Send your sample request to orders@solisbiodyne.com
PRODuCt CAT. NO. RXN/20 µl SIZE in ml
04-34-00S20 (free sample) 25 0.1
HOt FIREPol® MultiPlex Mix with
04-34-00120 250 1
10 mM MgCl2
04-34-02020 5000 20
04-36-00S20 (free sample) 25 0.1
HOt FIREPol® MultiPlex Mix
04-36-00120 250 1
Ready To Load with 10 mM MgCl2
04-36-02020 5000 20
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.com10
HOT FIREPol® Blend Master Mix &
Hot-start PCR Mixes
Blend Master Mix
Ready To Load
AMPLICONS OF VARIOUS LENGTH
Description FROM DIFFERENT TEMPLATES
lines 1-4 present an excellent amplification of fragments
a ready-to-use 5x-concentrated solution for more demanding endpoint of various length from λ DNA. Lines 5 and 6 show two
PCR assays. In addition to the hot-start taq polymerase HOt FIREPol® different amplicons amplified from mouse genomic DNa.
all these reactions were carried out using HOt FIREPol®
this master mix contains a proofreading enzyme which offers enhanced Blend Master Mix Ready to load with 7.5 mM MgCl2.
fidelity and performance. the mix has regular and Ready to load version
and different MgCl2 options for easier optimization. Ready to load mix
M 1 2 3 4 5 6
includes loading dyes for direct loading to gel.
Benefits
• increased yield, sensitivity and specificity
• up to 5x higher fidelity
• suitable for templates up to 5 kb
• reduced primer dimer formation
• Ready To Load mix available
• reaction set-up and shipment without ice
Did you know?
Lane Template Amplicon Length
Fidelity is the accuracy of the DNA
1 λ DNA 499 bp
polymerase at incorporating the correct
2 λ DNA 1003 bp
dNTP to the elongating DNA strand. 3 λ DNA 1998 bp
4 λ DNA 4991 bp
5 Mouse genomic DNA 808 bp
6 Mouse genomic DNA 3838 bp
Researchers already trust Selected publications:
Blend Master Mix • Herren, J.K. et al., Nat. Commun. (2020)
• Pent, M. et al., ISME J. (2020)
Reference:
• Popovici, J. et al., Nat. Commun. (2020)
“
• Bahram, M. et al., New Phytol. (2020)
Solis BioDyne has proven to be a great company that
• Roesch, C. et al., J. antimicrob. Chemother.
has customer-oriented services in molecular work. HOT
(2020)
FIREPol Blend Master Mix is convenient to use, store
• Prakash, C. et al., Sci. Rep. (2020)
and produces high quality results. I know what I am
• Piirsoo, A. et al., J. Virol. (2020)
saying because it outperforms same products from other
”
companies.
EMMY CHEPKOECH
PhD student
University of Eldoret, KenyaPRODuCt CatalOguE 2021 11
Send your sample request to orders@solisbiodyne.com
Hot-start PCR Mixes
PRODuCt CAT. NO. RXN/20 µl SIZE in ml
04-27-00S15 (free sample) 25 0.1
HOt FIREPol® Blend Master Mix
04-27-00115 250 1
with 7.5 mM MgCl2
04-27-02015 5000 20
04-27-00S20 (free sample) 25 0.1
HOt FIREPol® Blend Master Mix
04-27-00120 250 1
with 10 mM MgCl2
04-27-02020 5000 20
04-27-00S25 (free sample) 25 0.1
HOt FIREPol® Blend Master Mix
04-27-00125 250 1
with 12.5 mM MgCl2
04-27-02025 5000 20
Send your sample request to orders@solisbiodyne.com
PRODuCt CAT. NO. RXN/20 µl SIZE in ml
HOt FIREPol®
04-25-00S15 (free sample) 25 0.1
Blend Master Mix
04-25-00115 250 1
Ready To Load
04-25-02015 5000 20
with 7.5 mM MgCl2
HOt FIREPol®
04-25-00S20 (free sample) 25 0.1
Blend Master Mix
04-25-00120 250 1
Ready To Load
04-25-02020 5000 20
with 10 mM MgCl2
HOt FIREPol®
04-25-00S25 (free sample) 25 0.1
Blend Master Mix
04-25-00125 250 1
Ready To Load
04-25-02025 5000 20
with 12.5 mM MgCl2
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.com12
AMPLICONS OF VARIOUS GC-CONTENT
HOT FIREPol GC ®
Hot-start PCR Mixes
12 gC-rich genes were amplified from human gDNa
using HOt FIREPol® gC Master Mix. Final concentration
Master Mix of DNa template and DMSO was 1 ng/µl and 10%
respectively. the Master Mix performed well on
templates with up to 79% gC content.
M 1 2 3 4 5 6 7 8 9 10 11 12
Description
Q4ZIN3 763bp 71%
SOX18 798 bp 76%
B4GN4 719bp 78%
JUND 737bp 64.6%
PO3F3 790bp 78%
BAIP3 788bp 65%
Q8N4 727bp 78%
LMX1 737bp 68%
HES5 753bp 69%
ENST 733bp 77%
a ready-to-use 5x-concentrated solution for endpoint PCR assays,
RET 392bp 79%
NP 758bp 72%
optimised for gC-rich templates. It contains a hot-start taq DNa
polymerase HOt FIREPol® and allows amplification of up to 5 kb DNa
templates. Separate tubes of 25 mM MgCl2 and an additive for difficult
templates (100% DMSO) are supplied with the mix.
Benefits
• excellent amplification with templates up to 79% GC content
• suitable for templates up to 5 kb
• vials of 100% DMSO and 25 mM MgCl2 enable flexibility AMPLICONS OF VARIOUS LENGTHS FROM
in reaction optimization GC-RICH TEMPLATE
• reaction set-up and shipment without ice gC-rich fragments of various length from human gDNa
B4gN4 gene were amplified with HOt FIREPol® gC Master
Mix. Final concentration of DNa template and DMSO was
1 ng/µl and 10% respectively. the Master Mix performed
well with fragments of up to 3000 bp in length.
Did you know?
M 1 2 3 4 5
GC-rich templates need a special PCR master mix
because GC-rich DNA is more difficult to amplify.
It forms stable secondary structures that are more
3041 bp 68%
2573 bp 69%
2002 bp 72%
1569 bp 72%
719 bp 78%
resistant to denaturing and may cause unspecific
amplification.
Researchers already trust
GC Master Mix
Reference:
“
In our lab, GC Master mix gave excellent results with low-
abundance, difficult-to-amplify targets. Afterwards, these
PCR products were cloned into expression vectors and
sequenced - and vast majority of sequences were intact.
So the GC Master mix has low mutation rate and is a good
”
cloning tool as well.
DR ILLAR PATA Selected publications:
IVEX Lab, Estonia • Nahrung, H.F. et al., austral Entomol. (2020)
• O’Callahan, D. et al., Curr. Microbiol. (2020)
Send your sample request to orders@solisbiodyne.com
PRODuCt CAT. NO. RXN/20 µl SIZE in ml
04-33-00S15 (free sample) 25 0.1
HOt FIREPol®
04-33-00115 250 1
gC Master Mix
04-33-02015 5000 20
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.comPRODuCt CatalOguE 2021 13
PLANT GENOMIC DNA
FIREPol Master Mix ®
Standard PCR Mixes
672 bp fragment was amplified from barley genomic
DNa using FIREPol® Master Mix (lane 1-3) and FIREPol®
& Master Mix Master Mix Ready to load (lane 4-6). template DNa
was used at three tenfold dilutions starting from 1 ng/μl.
the Master Mixes performed well even at a template
Ready To Load concentration as low as 0.01 ng/µl.
M 1 2 3 4 5 6
Description
a ready-to-use 5x-concentrated solution for routine endpoint PCR
assays. It contains a thermostable taq DNa polymerase FIREPol® and
allows amplification of up to 5 kb DNa templates. the mix has two
versions - regular and Ready to load mix which includes loading dyes
for direct loading to gel.
Selected publications:
Benefits • touray, a.O. et al., Sci. Rep. (2020)
• Hassan, J. et al., Microorganisms. (2020)
• all-in-one master mix format reduces pipetting errors and saves time • Richards-Rios, P. et al., appl. Environ. Micro-
• suitable for templates up to 5 kb biol. (2020)
• Ready To Load mix available for direct loading to gel • Bielik, V. et al., ann. Nutr. Metab. (2020)
• reaction set-up and shipment without ice • Yehia, H.M. et al., J. Dairy Sci. (2020)
• Furlanis, E. et al., Nat. Neurosci. (2019)
• Li, N. et al., J. Endocrinol. (2019)
Researchers already trust
FIREPol® Master Mix
Reference:
Did you know?
“
We are working with FIREPol Master Mix for few years now
®
MgCl2 acts as a cofactor
and we are very satisfied. We are using it for genotyping and is a catalyzer in PCR
(many different PCR) and we have excellent results. The reaction. Mg2+ ions bind
”
Ready To Load format is very time saving. to the catalytic site of
the DNA polymerase and
catalyze phosphodiester
CLAUDINE CORNELOUP bond formation between
Engineer assistant the two dNTPs.
Platform for Experimental Biology on Mice,
Genotyping Laboratory, ENS de Lyon, France
Send your sample request to orders@solisbiodyne.com
PRODuCt CAT. NO. RXN/20 µl SIZE in ml
04-11-00S15 (free sample) 25 0.1
FIREPol® Master Mix with 7.5 mM MgCl2
04-11-00115 250 1
04-11-00S25 (free sample) 25 0.1
FIREPol® Master Mix with 12.5 mM MgCl2
04-11-00125 250 1
FIREPol® Master Mix 04-12-00S15 (free sample) 25 0.1
Ready To Load with 7.5 mM MgCl2 04-12-00115 250 1
FIREPol® Master Mix 04-12-00S25 (free sample) 25 0.1
Ready To Load with 12.5 mM MgCl2 04-12-00125 250 1
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.com14
HIGHLY COMPETITIVE
HOT FIREPol ®
PCR Enzymes
Four fragments from human gDNa were amplified in multi-
plex reaction using HOt FIREPol® DNA Polymerase (lane 1-2)
DNA Polymerase and two other hot start enzymes from company A (lane
3-4) and company B (lane 5-6). HOt FIREPol® DNA
Polymerase performed well with all four fragments in
both 10x dilutions.
Description
M 1 2 3 4 5 6
a chemically modified hot-start version of a thermostable taq DNa
polymerase FIREPol®. This enzyme is activated only after heat treatment
at 95°C which prevents any unspecific polymerase activity at lower
3041 bp 68%
2573 bp 69%
2002 bp 72%
1569 bp 72%
temperatures during reaction set-up. HOt FIREPol® DNA polymerase is
719 bp 78%
supplied with 2 reaction buffers, 25 mM MgCl2 and an additive for difficult
templates. HOt FIREPol® 10x Buffer B2 contains non-ionic detergent
suppressing inhibitory effects of the traces of DNa extraction buffers
and enhancing PCR yield and efficiency.
Benefits
• increased specificity and sensitivity
• reduced primer dimer formation
• suitable for TA cloning
• reaction buffer with and without detergent included
• Solution S included in a separate vial for GC-rich templates
• MgCl2 included in a separate vial
• reaction set-up and shipment without ice
Did you know?
Our polymerases and master mixes are compatible
with a downstream restriction enzyme digest
without cleaning up the PCR reaction.
Researchers already trust HOT FIREPol® Selected publications:
• Delavaux, C.S. et al., ISME J. (2020)
Reference:
• Kavazović, I. et al., PlOS Biol. (2020)
“
• Kamboj, N. et al., Mater. Sci. Eng. C. (2020)
We had some problems with the implementation of a
• amorim, J.P. et al., Cell Rep. (2020)
protocol, we tried for a long time with different enzymes • Kluckova, D. et al., Sci. Rep. (2020)
without any positive results. We tested the HOT FIREPol® • Schreurs, R.R.C.E. et al., Immunity. (2019)
and it was the perfect troubleshooting, besides the great
”
• tessema, S.K. et al., Cell Host Microbe. (2019)
technical support received from Solis BioDyne. • Stavusis, J. et al., Ann. Neurol. (2019)
DR MARIA JOSE SUAREZ
CIHATA, University of Costa Rica
Send your sample request to orders@solisbiodyne.com
PRODuCt CAT. NO. SIZE in U
01-02-0000S (free sample) 100
HOt FIREPol® 01-02-00500 500
DNa Polymerase (5 u/µl) 01-02-01000 1000
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.comPRODuCt CatalOguE 2021 15
MOUSE GENOMIC DNA
FIREPol DNA Polymerase ®
PCR Enzymes
1200 bp fragment of Beta-synuclein gene was amplified
from mouse genomic DNa using FIREPol® DNA Polymerase
with two different buffers: B (lane 1-3) and BD (lane
4-6). Template DNA was used at three tenfold dilutions
starting from 1 ng/μl. FIREPol® DNA Polymerase was
used at 0.04 u/µl.
Description M 1 2 3 4 5 6
A genetically modified thermostable Taq DNA polymerase that
provides robust and reproducible results. FIREPol® DNA polymerase
is supplied with 2 reaction buffers, 25 mM MgCl2 and an additive for
difficult templates. FIREPol® 10x Buffer B contains non-ionic detergent
suppressing inhibitory effects of the traces of DNa extraction buffer
and enhancing PCR yield and efficiency.
Benefits
M 1 2 3 4 5 6
• robust amplification for routine applications
• suitable for templates up to 5 kb
• suitable for TA cloning M 1 2 3 4 5 6
• reaction buffers with and without detergent included
PLANT GENOMIC DNA
• Solution S included in a separate vial for GC-rich templates
672 bp fragment was amplified from barley genomic
• MgCl2 included in a separate vial DNa using FIREPol® DNA Polymerase with two buffers:
• reaction set-up and shipment without ice B (lane 1-3) and BD (lane 4-6). Template DNA was
used at three tenfold dilutions starting from 1 ng/μl. the
enzyme performed well even at a template concentration
Reference: as low as 0.01 ng/µl. FIREPol® DNA Polymerase was
used at 0.04 u/µl.
“
I found that for FIREPol® DNA Polymerase the quality
was comparable to similar products even though the price M 1 2 3 4 5 6
was much cheaper for the Solis product. Therefore, Solis
BioDyne are head and shoulders above their competitors
when it comes to value for money which is especially
important given the funding situation in these straitened
”
times.
DR. GARY LOUGHRAN
Research Fellow Selected publications:
School of Biochemistry and Cell Biology • Sadek, M. et al., antimicrob. agents
University College Cork, Ireland Chemother. (2020)
• Rodriguez-Campos, S. et al., Sci. Rep. (2020)
• Barna, L. et al., Fluids Barriers CNS. (2020)
• Šeligová, B. et al., Helicobacter. (2020)
Tip! • Doma, A.O. et al., Antibiotics. (2020)
• Hummitzsch, l. et al., Stem Cells Int. (2019)
During PCR cycling, keep your primer • Chanchaithong, P. et al., J. Antimicrob.
annealing temperature 2-5°C below the Tm Chemother. (2019)
of the primer having the lowest Tm. • Müller, P. et al., RNa Biol. (2019)
Send your sample request to orders@solisbiodyne.com
PRODuCt CAT. NO. SIZE in U
01-01-0000S (free sample) 100
FIREPol® DNA Polymerase 01-01-00500 500
(5 u/µl) 01-01-01000 1000
01-01-02000 2000
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.com16
qPCR Mixes: Product Selection Guides
qPCR Mixes: Product Selection guides
Mixes for dye-based qPCR assays
gC-rich Visuali-
Speed Sensitivity dUTP Page
performance zation dye
NEW! SolisFAST® SolisGreen® qPCR Mix *** ** * 18
HOT FIREPol® SolisGreen® qPCR Mix * *** * 19
HOT FIREPol® EvaGreen® qPCR Supermix * ** *** • • 20
HOT FIREPol® EvaGreen® qPCR Mix Plus * * * 21
HOT FIREPol® EvaGreen® HRM Mix * *** * 22
For Cycler Compatibility, please check qPCR Mix compatibility table for dye-based mixes on page 17.
Mixes for probe-based qPCR assays
gC-rich Multiplex
Speed dUTP uNg Page
performance qPCR
NEW! SolisFAST® Probe Mix *** * ≤ 5 targets 24-25
NEW! SolisFAST® Probe Mix with UNG* *** * ≤ 5 targets • • 24-25
HOT FIREPol® Probe Multiplex qPCR Mix * *** ≤ 4 targets • 26
HOT FIREPol® Probe Universal qPCR Mix * *** ≤ 2 targets • 27
HOT FIREPol® Probe qPCR Mix Plus * * ≤ 2 targets 28
* SolisFAST® Probe qPCR Mixes with uNg available upon request.
For cycler compatibility, please check qPCR mix compatibility table for probe-based mixes on page 23.PRODuCt CatalOguE 2021 17
qPCR Mix Compatibility Table:
Dye-based qPCR: Comatibility table
Dye-based qPCR Mixes
Fast cycling Standard cycling HRM
HOt FIREPol® Solisgreen®
HOt FIREPol® Evagreen®
HOt FIREPol® Evagreen®
HOt FIREPol® Evagreen®
HOt FIREPol® Evagreen®
HOt FIREPol® Evagreen®
HOt FIREPol® Evagreen®
qPCR Mix Plus (Capillary)
qPCR Mix Plus (no ROX)
SolisFAST® Solisgreen®
SolisFAST® Solisgreen®
qPCR Mix Plus (ROX)
qPCR Mix (no ROX)
HRM Mix (no ROX)
qPCR Mix (ROX)
HRM Mix (ROX)
qPCR Supermix
qPCR Mix
Applied Biosystems
5700, 7000, 7300, 7700,
7900Ht, 7900Ht Fast, • • •
StepOne™, StepOnePlus™
7500, 7500 Fast, Viia™7,
QuantStudio™ 3, 5, 6 Flex, 7 Flex, • • • • •
12K Flex
Agilent/Stratagene
Mx3000P™, Mx3005P™,
Mx4000™ • • • • •
Bio-Rad
CFX96™, CFX384™ • • • • •
iQ™5, MyiQ™, Chromo4™,
Opticon®2; MiniOpticon® • • • •
Bio Molecular Systems (BMS)
Mic • • • • •
Eppendorf
Mastercycler® ep Realplex • • • •
Qiagen
Rotor-gene® 3000, Rotor-gene®
6000, Rotor-gene® Q
• • • • •
Thermo Scientific
PikoReal™ • • • • •
Illumina
the Eco™ • • • • •
Roche Applied Science
LightCycler® 480, LightCycler®
Nano, LightCycler® 96
• • • • •
LightCycler® 1.x, 2.0 •
Takara
thermal Cycler Dice™ (tP800) • • • • •18
ACCURATE AND SENSITIVE qPCR
SolisFAST SolisGreen ® ®
Dye-based qPCR Mixes
amplification of a 75 bp fragment of B2M gene using five
tenfold dilutions of human cDNA (100 ng – 10 pg with
qPCR Mixes three replicates at each concentration) with SolisFAST®
Solisgreen® qPCR Mix. qPCR was performed on a Mic
qPCR cycler (Bio Molecular Systems). thermal conditions:
activation 30 sec at 95°C, cycling 5 sec at 95°C, 20 sec
Description at 60°C.
Fast and sensitive ready-to-use 5x-concentrated solution for dye-based
qPCR detection of DNa targets using Solisgreen® dsDNA intercalating dye
6.31
Slope = -3.347
and SYBR®/FaM detection channel. the mix contains a novel SolisFaSt®
3.98
E = 99.6%
R2 = 0.9895
DNa Polymerase with fast hot-start and 2-4 times faster extension
2.51
Normalized Fluorescence
rates (15-30 sec/kb) compared to the wild-type taq DNa polymerase
1.58
(60 sec/kb), HOt FIREPol® and FIREPol® DNA polymerases. It has two
1.00
versions to match different instruments. the ROX-mix is compatible with
0.63
qPCR instruments that require low ROX level for signal normalization.
0.40
Solisgreen® qPCR mixes are suitable for commercial and diagnostic
0.25
applications and require no additional licensing.
5 10 15 20 25 30 35 40
Cycles
Benefits 0.18
0.16
• qPCR results 2x faster 0.14
• fast hot-start (30 sec-3 min)
0.12
0.1
• fast amplification (15-30 sec/kb)
-dF/dT
0.08
• bright and sensitive SolisGreen® dye 0.06
• suitable for commercial applications
0.04
0.02
• compatible with most cyclers, except high ROX 0
• different product versions: no ROX and ROX 75 80 85
Temperature (°C)
90
• reaction set-up and shipment without ice
RESULTS IN 30 MINUTES!
100
Duration of a qPCR run with 90
standard thermal conditions using
Did you know?
80
regular qPCR mix and fast thermal 70
Run time (min)
conditions using SolisFAST® 60
Solisgreen® qPCR Mix.
50
40
SolisGreen® and
30 EvaGreen® dyes are
20
detected in the same
10
0 channel as SYBR® Green I.
LightCycler 480
(Roche)
CFX96
(Bio-Rad)
MIC
(Bio Molecular
You don't have to change
Systems)
any detection settings on
Standard qPCR reaction your qPCR cycler.
Fast / ultra Fast qPCR reaction
Send your sample request to orders@solisbiodyne.com
PRODuCt CAT. NO. RXN/20 µl SIZE in ml
28-41-0000S (free sample) 50 0.2
SolisFAST® Solisgreen® qPCR Mix 28-41-00001 250 1
(no ROX) 28-41-00001-5 5 x 250 5x1
28-41-00020 5000 20
28-46-0000S (free sample) 50 0.2
SolisFAST® Solisgreen® qPCR Mix 28-46-00001 250 1
(ROX) 28-46-00001-5 5 x 250 5x1
28-46-00020 5000 20
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.comPRODuCt CatalOguE 2021 19
HIGHLY COMPETITIVE
HOT FIREPol ®
Dye-based qPCR Mixes
amplification of six tenfold dilutions of human gaPDH
cDNa with HOt FIREPol® Solisgreen® qPCR Mix (red)
SolisGreen® qPCR Mix and a qPCR mix from another vendor (green). Reactions
were performed on an applied BioSystems™ Quant-
Studio™ 6 Flex.
Description
a highly sensitive ready-to-use 5x reaction mix for dye-based qPCR
detection of DNa targets using Solisgreen® dsDNA intercalating dye
and SYBR®/FaM detection channel. the mix has excellent performance
with low template amounts. It contains an internal reference based on
ROX dye and is compatible with qPCR instruments that require low ROX
level for signal normalization. Solisgreen® qPCR mixes are suitable for
commercial and diagnostic applications and require no additional licensing. Solis BioDyne Competitor B
Benefits EXCELLENT SENSITIVITY
amplification of human PPIa cDNa with HOt FIREPol®
• enhanced performance with low DNA concentrations Solisgreen® qPCR Mix on an applied BioSystems™
• bright and sensitive SolisGreen® dye QuantStudio™ 6 Flex shows excellent linearity across
• suitable for commercial applications different DNA concentrations. The amount of cDNA per
reaction ranges from 100 ng to 0.1 pg.
• one qPCR mix for all cyclers, except high ROX
• reaction set-up and shipment without ice
Slope: -3.458
R2: 0.998
Researchers already trust SolisGreen®
Reference:
“
Excellent product quality along with affordable prices and
100 ng 10 ng 1 ng 0.1 ng
committed customer service: these are the reasons why Solis
10 pg 1 pg 0.1 pg
BioDyne is our strategic enzyme supplier since many years.
Now we continue our partnership with the robust, stable
and very sensitive SolisGreen mixes for our qPCR product
”
platform.
Did you know?
Compared to SYBR® Green I,
DAVIDE ROASCHIO
our next generation dyes
Scientist in Product Development
SolisGreen® and EvaGreen®
Loewe Biochemica GmbH, Germany
have:
• higher fluorescence level
• higher sensitivity for
detecting low template
concentrations
• higher stability
Send your sample request to orders@solisbiodyne.com
PRODuCt CAT. NO. RXN/20 µl SIZE in ml
08-46-0000S (free sample) 50 0.2
08-46-00001 250 1
HOt FIREPol® Solisgreen® qPCR Mix
08-46-00008 2000 8
08-46-00020 5000 20
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.com20
TRUSTWORTHY PERFORMANCE
HOT FIREPol EvaGreen ® ®
Dye-based qPCR Mixes
amplification plots of tenfold dilution series for the human
gaPDH gene performed on applied Biosystems™ Viia™7
qPCR Supermix (upper graph) and Roche lightCycler® 480 (lower graph).
The amount of DNA per reaction ranges from 0.01 to 10
ng. the results show high linear range and high efficiency
across a wide range of DNA concentrations on different
qPCR platforms.
Description
a universal ready-to-use 5x-concentrated solution for dye-based qPCR 1E01
detection of DNa targets using Evagreen® dsDNA intercalating dye and
1E00
SYBR®/FaM detection channel. the mix is optimised for highly specific
results and reduced primer-dimer formation with excellent amplification of
ΔRn
0,2
0.2 Efficiency: 97.9
gC-rich regions. the Mix contains dutPs to prevent cross-contamination 0.1
Slope: -3.37
when used with uNg treatment, and a visible dye to ease reaction set-up. R2:1
It contains an internal reference based on ROX dye and is compatible 0.01
18 20 22 24 26 28 30 32 34 36 38 40 42 44
with most qPCR instruments, including those that require low and high Cycle Number
ROX level for signal normalization.
31.225
Benefits
Fluorescence (465-510)
• high sensitivity with low DNA concentrations 21.225
• robust amplification of GC-rich targets
• blue visualization dye to ease pipetting Efficiency: 101,25
11.225 Slope: -3.263
• reduced primer dimer formation
• contains dUTP to prevent cross-contamination when used in
combination with uNg 1.225
• one qPCR mix for all cyclers (except capillary) 18 20 22 24 26 28 30 32 34 36 38 40 42 44
• reaction set-up and shipment without ice Cycle Number
Did you know?
The average GC-content
in human genome ranges
from 35% to 60% across
100 kb fragments,
Selected publications: with a mean of 46.1%.
• Hégarat, N. et al., EMBO J. (2020) GC-content above 60% is
• Santa-Marinha, l. et al., Cell Rep. (2020) considered as high GC.
• Dahlem, C. et al., Cancers (Basel). (2020)
• Valbuena Perez, J.V. et al., Aging Cell. (2020)
• Vaid, R. et al., Nucleic acids Res. (2020)
• Ventura, R.D. et al., Free Radic. Biol. Med. (2020)
• Carfagna, I.E. et al., Mol. Immunol. (2020)
• Roesch, C. et al., J. antimicrob. Chemother. (2020)
• Singh, R.K. et al., Int. J. Mol. Sci. (2020)
• Pasin, F. et al., Plant Commun. (2020)
• Noelia, M. et al., Biochem. Pharmacol. (2020)
Send your sample request to orders@solisbiodyne.com
PRODuCt CAT. NO. RXN/20 µl SIZE in ml
08-36-0000S (free sample) 50 0.2
HOt FIREPol® Evagreen® 08-36-00001 250 1
qPCR Supermix 08-36-00008 2000 8
08-36-00020 5000 20
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.comPRODuCt CatalOguE 2021 21
EXCELLENT SENSITIVITY AND SPECIFICITY
HOT FIREPol EvaGreen ® ®
Dye-based qPCR Mixes
the amplification of a 98 bp fragment of gaPDH gene
exhibits sensitive and efficient reaction curves (upper
qPCR Mix Plus graph) with highly specific peak in melt curve analysis
(lower graph) using HOt FIREPol® Evagreen® qPCR Mix
Plus (no ROX). amplification was performed on human
genomic DNa using Rotor-gene® 6000 qPCR cycler
following cycling protocols recommended by the supplier.
Description
10
a cost-effective ready-to-use 5x-concentrated solution for dye-based
qPCR detection of DNa targets using Evagreen® dsDNA intercalating
Norm. fluoro.
dye and SYBR®/FaM detection channel. the mix has three versions to 1
match different instruments. the ROX-mix is compatible with qPCR
instruments that require low or high ROX level for signal normalization. 0.2
the capillary-mix is optimised for capillary based systems. 0.1
Benefits 0.01
15 17 19 21 23 25 27 29 31 33 35
Cycle Number
• cost-effective solution for less demanding assays
• suitable for most dye-based applications 2.8
• high sensitivity and specificity 2.4
• excellent efficiency 2
• compatible with most cyclers
dF / dT
1.6
• different product versions: no ROX, ROX, capillary 1.2
• reaction set-up and shipment without ice 0.8
0.4
0
66 70 74 78 82 86 90 94
°C
Selected publications: Tip!
• Hégarat, N. et al., EMBO J. (2020)
Analyze your primers for
• Santa-Marinha, l. et al., Cell Rep. (2020)
self-complementarity and
• Dahlem, C. et al., Cancers (Basel). (2020)
• Valbuena Perez, J.V. et al., Aging Cell. (2020)
stable secondary structures
• Vaid, R. et al., Nucleic acids Res. (2020) (e.g. hairpins) in their
• Ventura, R.D. et al., Free Radic. Biol. Med. (2020) sequences. Avoid the 3'-self
• Carfagna, I.E. et al., Mol. Immunol. (2020) complementarity, because
• Roesch, C. et al., J. antimicrob. Chemother. (2020) it increases possibility of
• Singh, R.K. et al., Int. J. Mol. Sci. (2020) primer-dimers formation.
• Pasin, F. et al., Plant Commun. (2020)
• Noelia, M. et al., Biochem. Pharmacol. (2020)
Send your sample request to orders@solisbiodyne.com
PRODuCt CAT. NO. RXN/20 µl SIZE in ml
08-24-0000S (free sample) 50 0.2
HOt FIREPol® Evagreen® qPCR Mix Plus 08-24-00001 250 1
(ROX) 08-24-00008 2000 8
08-24-00020 5000 20
08-25-0000S (free sample) 50 0.2
HOt FIREPol® Evagreen® qPCR Mix Plus 08-25-00001 250 1
(no ROX) 08-25-00008 2000 8
08-25-00020 5000 20
08-26-0000S (free sample) 50 0.2
HOt FIREPol® Evagreen® qPCR Mix Plus 08-26-00001 250 1
(Capillary) 08-26-00008 2000 8
08-26-00020 5000 20
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.com22
SENSITIVE HRM GENOTYPING
HOT FIREPol EvaGreen ® ®
Dye-based qPCR Mixes
High Resolution Melt analysis was used to genotype a C
insertion in BRCa1 gene, a breast cancer susceptibility
HRM Mix gene, with HOt FIREPol® Evagreen® HRM Mix (two
graphs below). Reactions were performed on Corbett
Rotor-gene® 6000. green lines represent wildtypes
without an insertion, red lines represent a C insertion and
blue line represents a patient with unknown phenotype.
Description
a ready-to-use 5x-concentrated solution for High Resolution Melt (HRM)
90
analysis of DNa targets using Evagreen® dsDNA intercalating dye and
Normalized Fluorescence
SYBR®/FaM detection. the mix has two versions to match different 70
instruments. the ROX-mix is compatible with qPCR instruments that
require low or high ROX level for signal normalization. 50
Benefits 30
• excellent resolution in HRM assays allows detection 10
of DNA sequence variations 77 78 79 80 81 82 83
°C
• contains sensitive EvaGreen® dye
Normalized minus 5382 mut insC
• compatible with most cyclers 12
• different product versions: no ROX, ROX 10
• reaction set-up and shipment without ice 8
6
4
2
Did you know? 0
-2
High resolution melt analysis can be used 77 78 79 80 81 82 83
for SNP genotyping, discovering mutations, °C
screening for heterozygosity, analyzing No. Color Name Genotype Confi. %
DNA methylation. 37 Unknown 5382 wt 99.18
phenotype
40 Wildtype 1 5382 wt 97.33
41 Wildtype 2 5382 wt 100.00
42 Mutation 1 5382 mut insC 100.00
43 Mutation 2 5382 mut insC 97.47
Selected publications:
• Hégarat, N. et al., EMBO J. (2020)
• Santa-Marinha, l. et al., Cell Rep. (2020)
• Dahlem, C. et al., Cancers (Basel). (2020)
• Valbuena Perez, J.V. et al., Aging Cell. (2020)
• Vaid, R. et al., Nucleic acids Res. (2020)
• Ventura, R.D. et al., Free Radic. Biol. Med. (2020)
• Carfagna, I.E. et al., Mol. Immunol. (2020)
• Roesch, C. et al., J. antimicrob. Chemother. (2020)
• Singh, R.K. et al., Int. J. Mol. Sci. (2020)
• Pasin, F. et al., Plant Commun. (2020)
• Noelia, M. et al., Biochem. Pharmacol. (2020)
Send your sample request to orders@solisbiodyne.com
PRODuCt CAT. NO. RXN/20 µl SIZE in ml
08-33-0000S (free sample) 50 0.2
HOt FIREPol® Evagreen® 08-33-00001 250 1
HRM Mix (ROX) 08-33-00008 2000 8
08-33-00020 5000 20
08-31-0000S (free sample) 50 0.2
HOt FIREPol® Evagreen® 08-31-00001 250 1
HRM Mix (no ROX) 08-31-00008 2000 8
08-31-00020 5000 20
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.comPRODuCt CatalOguE 2021 23
qPCR Mix Compatibility Table:
Probe-based qPCR: Mix Compatibility table
Probe-based qPCR Mixes
Fast cycling Standard cycling
qPCR Mix Plus (Capillary)
qPCR Mix Plus (no ROX)
SolisFAST® Probe qPCR
SolisFAST® Probe qPCR
HOt FIREPol® Multiplex
HOt FIREPol® Multiplex
HOt FIREPol® Multiplex
qPCR Mix Plus (ROX)*
universal qPCR Mix*
HOt FIREPol® Probe
HOt FIREPol® Probe
HOt FIREPol® Probe
HOt FIREPol® Probe
qPCR Mix (Purple)**
qPCR Mix (ROX)*
Mix (no ROX)
Mix (ROX)*
qPCR Mix
Applied Biosystems
5700, 7000, 7300, 7700,
7900Ht, 7900Ht Fast, • • • •
StepOne™, StepOnePlus™
7500, 7500 Fast, Viia™7,
QuantStudio™ 3**, 5, 6 Flex, 7 • • • • •
Flex, 12K Flex
Agilent/Stratagene
Mx3000P™, Mx3005P™,
Mx4000™ • • • •
Bio-Rad
CFX96™, CFX384™ • • • •
iQ™5, MyiQ™, Chromo4™,
Opticon®2; MiniOpticon® • • • •
Bio Molecular Systems (BMS)
Mic • • • •
Eppendorf
Mastercycler® ep Realplex • • • •
Qiagen
Rotor-gene® 3000, Rotor-gene®
6000, Rotor-gene® Q • • • •
Thermo Scientific
PikoReal™ • • • •
Illumina
the Eco™ • • • •
Roche Applied Science
LightCycler® 480, LightCycler®
Nano, LightCycler® 96 • • • •
LightCycler® 1.x, 2.0 •
Takara
thermal Cycler Dice™ (tP800) • • • •
SolisFAST® Probe qPCR Mixes with Purple reference dye and/or with uNg enzyme are available upon request.
* Mixes that contain ROX can not be used with ROX, JuN and texas Red labelled probes.
** Mixes with Purple reference dye are not compatible with applied Biosystems QuantStudio™ 324
ACCURATE AND SENSITIVE qPCR
SolisFAST Probe ®
Probe-based qPCR Mixes
amplification of a 101 bp fragment of PPIA gene using
six tenfold dilutions of human cDNa (100 ng – 1 pg, three
qPCR Mixes replicates at each concentration). qPCR was performed
on a CFX96™ qPCR cycler (Bio-Rad) using SolisFaSt®
Probe qPCR Mix (no ROX), with detection in FaM channel.
Thermal conditions: activation 30 sec at 95°C, cycling
Description 2 sec at 95°C, 10 sec at 60°C.
Fast and sensitive ready-to-use 5x-concentrated solution for probe-based
qPCR detection of DNa targets using taqMan® and other hydrolysis probe Slope = -3.314
E = 100.3%
R2 = 1.000
types. the mix contains a novel SolisFaSt® DNA Polymerase with fast
hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared
to the wild-type taq DNa polymerase (60 sec/kb), HOt FIREPol® and
FIREPol® DNA polymerases, and is optimised for sensitive detection of
up to 5 targets in one reaction. It has three versions to match different
instruments and assay requirements. ROX-mix is compatible with qPCR
instruments that require low and high ROX level for signal normalization,
Purple-mix is compatible with instruments that use Mustang Purple™ EXCELLENT FOR MULTIPLEX ASSAYS
for signal normalisation. dutP and uNg containing mixes are available Four-plex qPCR amplification with four tenfold serial
upon request to prevent carry-over contamination. dilutions of human gDNA (40 ng – 40 pg, three replicates
at each concentration). qPCR was performed on a
QuantStudio™ 6 Flex qPCR cycler (applied BioSystems™)
Benefits with SolisFAST® Probe qPCR Mix (ROX) using ROX dye
for normalization. Thermal conditions: activation 3 min at
• qPCR results 2x faster 95°C, cycling 5 sec at 95°C, 20 sec at 60°C.
• fast hot-start (30 sec-3 min)
• fast amplification (15-30 sec/kb)
• analyze 1-5 targets in 1 reaction
• mix with dUTP and UNG available to prevent
carry-over contamination
• compatible with most cyclers
• different product versions: no ROX, ROX, Purple
• reaction set-up and shipment without ice
FaM ABY VIC Cy5
Did you know?
You can avoid carry-over
contamination using our UNG
Mixes containing dUTPs and
UNG enzyme
100 2X LESS TIME FROM SAMPLE TO RESULTS
90
80 Duration of a 40-cycle qPCR run with standard thermal
70 conditions using regular qPCR mix (initial activation 10-12
60
Run time (min)
50
min; denaturation 15 sec, annealing/extension 40-60 sec)
40 and fast thermal conditions using SolisFAST® Probe qPCR
30
Master Mix (initial activation 2-3 min; denaturation 2-5
20
10 sec, annealing extension 10-20 sec). amplifications were
0
Standard qPCR reaction performed on human gDNA.
MIC CFX96 QuantStudio
(Bio Molecular (Bio-Rad) 6 Flex Fast qPCR reaction
Systems) (Applied
Biosystems)PRODuCt CatalOguE 2021 25
Send your sample request to orders@solisbiodyne.com
Probe-based qPCR Mixes
PRODuCt* CAT. NO. RXN/20 µl SIZE in ml
28-01-0000S (free sample) 50 0.2
28-01-00001 250 1
SolisFAST Probe qPCR Mix (no ROX)
®
28-01-00001-5 5 x 250 5x1
28-01-00020 5000 20
28-02-0000S (free sample) 50 0.2
28-02-00001 250 1
SolisFAST® Probe qPCR Mix (ROX)
28-02-00001-5 5 x 250 5x1
28-02-00020 5000 20
28-03-0000S (free sample) 50 0.2
28-03-00001 250 1
SolisFAST Probe qPCR Mix (Purple)**
®
28-03-00001-5 5 x 250 5x1
28-03-00020 5000 20
* SolisFAST® Probe qPCR Mixes with uNg available upon request
** SolisFAST® Probe qPCR Mixes with Purple dye available upon request
Products and samples
can be ordered via e-mail: orders@solisbiodyne.com, via skype: solis.biodyne, via phone: +372 740 9960, or via our e-shop: solisbiodyne.comYou can also read
