PRESENTATION DU PROJET ERC REVOLINC - Jérémy Bouyer Lancement du Réseau Vectopôle Sud Journée d'Animation Scientifique " Stratégies Innovantes ...
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PRESENTATION DU PROJET ERC REVOLINC
Jérémy Bouyer
Lancement du Réseau Vectopôle Sud ‐ Journée d’Animation Scientifique
« Stratégies Innovantes de Lutte contre les Insectes Vecteurs et Ravageurs »
3 mai 2018 Agropolis International
1
THE CHALLENGE
2
Toxicity and Reduction of
ecotoxicity of authorized
insecticides insecticides
(by 75% in Europe)
Resurgences
Insecticide Diseases and
resistance pests outbreaks
Economical loses
Environmental Biological
changes invasions
(local/global) (vectors/pests)
3
INNOVATION
Toxicity and Reduction of
ecotoxicity of authorized
insecticides insecticides
(by 75% in Europe)
Resurgences
Insecticide Diseases and
resistance pests outbreaks
Economical loses
Environmental Biological
changes invasions
(local/global) (vectors/pests)
4
… worse in Europe!
Pyrethroids
DDT
Pyrethroids
Pyrethroids
Source: Justin McBeath ISNTD Bites – March 19th 2015 5
THE PRINCIPLE
6
SIT Boosted SIT
Bouyer & Lefrançois Trends Parasitol 2014
INSECT MODELS
8
PUBLIC HEALTH
Mosquitoes
Aedes albopictus
9
2010 2015
10Moustique tigre: « Appel à la vigilance » dans 42 départements métropolitains il y a 4
jours par la Direction Général de la Santé
Situation au 1er Janvier 2018
« surveillance renforcée »
11AGRICULTURE PUBLIC & ANIMAL HEALTH
Fruit flies Tsetse flies
Ceratitis capitata Glossina palpalis gambiensis
12THE TEAM
13Vectopôle
LISIS
.
14WHAT IS NEEDED TO RUN BOOSTED SIT?
WHAT WILL WE ADDRESS?
15Transfer of biocides during mating
and impact on female fertility
(PP & Bti & Densovirus)
Resilience and
+
transfer / mating Transfer breeding
Contamination sites & impact on
technique fecundity
16Survival of sterile males
Glossina palpalis gambiensis
100
Survival of sterile males (%)
90
35mg F15 + Pyriproxyfen
80
70
60 Control
50
40
30
20 Control F15
10
0
0H 6H 48H 4j 7j 9j 13j 15j 17j 21j
Time after treatment
No impact of PP on survival
Master2 Lison LarochePersistency of pyriproxyfen
Glossina palpalis gambiensis
Amount of PP per tsetse (ng)
1400
1200
1000
800
600
400
200
0
0H 3H 6H 24H 48H 72H 4J 6J 8J 10J
Time after treatment
Fast initial decline of PP followed by a stagnation
Master2 Lison LarocheTransfer of pyriproxyfen from males to females
Glossina palpalis gambiensis
Amount of pyriproxyfen per female (ng) 80
71,453
70
60
50 42,78
40
30
20 15,35 17,82 17,65 18,26
10
1,35
0
Nb of contacts without mating
Master2 Lison LarocheSurvival of mosquitoes treated with pyriproxyfen
Aedes albopictus / 40% PP
Reduced survival (+50% mortality in 14 days)Persistency of pyriproxyfen
Aedes albopictus
240
183,48
Amount of PP per individual (ng)
200
132,01
160
107,67
120
62,01
80 42,03
16,76
40
0
T0 (0 heure) T1 (3 T2 (6 T3 (24 T4 (48 T5 (4 jours)
heures) heures) heures) heures)
‐40
Time after treatmentWhat about associating SIT to
DENSOVIRUSES?
22Drawn by Steve Higgs 23
The Lancet Global Health 2016 4, DOI: (10.1016/S2214-109X(16)00082-6) Copyright © 2016 Bouyer et al. Open Access article distributed under the terms of CC BY-NC-ND Terms and Conditions
Mosquitoes Densoviruses
Ongoing trials
Production en cellules
(virus équivalent génome – Test d’infection
DVs moustiques Origine des DVs
veg/µl) sécrétion dans le en larves
surnageant
Aedes albopictus C6/36P chroniquement
Densovirus 2 infectées
Jousset et al., 1993 2,4 x 108 En cours
AalDV2
Récolte des surnageants de culture
Clone viral C6/36 3,4x106
origine: synthèse de
genes
AAG2
Production de virus par 1,2x103
Aedes aegypti transfection de culture de
Densovirus 1 cellules de moustiques
Clone viral C6/36 1,3 x 101
AaeDV1 origine : clonage génome
viral
Production de virus par AAG2 3,8x106 En cours
transfection de cultures
de cellules de moustiquesHigh specificity
Carlson et al. 2006
Advances in virus
research
26Aedes albopictus Densovirus 2 produced from infectious plasmid
Infection in Aedes albopictus and Aedes aegypti larvae
Protocol: Protocol:
• 1 larvae L1, P96 well, +/- virus • 1 larvae L1, P96 well, +/- virus
• Transfert 48hpi in P24 well plate + food • Virus +/- FB28 0,1%
• Transfert 48hpi in P24 well plate + food
Survival curve for Ae. albopictus larvae (Italian strain) Survival curve for Ae. aegypti larvae (Bora strain)
infected with AalDV2 virus infected with AalDV2 virusAedes albopictus Densovirus 2 produced from C6/36 chronically
infected cell line
Infection in Aedes albopictus larvae
Protocol:
• 100 larvaes L1, 5 ml cell culture
• Transfert 48hpi in 300 ml water + food
• Analysis 14 dpi
AalDV2 effects :
• ↗ mortality
• ↘ development
• ↗ cannibalism
Individuals number at 14 dpiImpact of boosted SIT in semi‐field
and field trials with the best biocide
(Ae. albopictus & C. capitata)
Semi‐field trials Experimental field trials
29Relative impacts of SIT and boosted‐SIT
on population dynamics and resilience
Deterministic models Individual‐based models
30Preliminary models with Pyriproxyfen
Elimination with minimised Rtotal:
RSIT opt = 2528
RBSIT opt = 1214
David Pleydell 31Preliminary models with Pyriproxyfen
David Pleydell 32Population dynamics of Ae. albopictus in
La Réunion (AlboRun)
Spatial model
Accounting for Climate
Validated on entomological data
Used by Agence Régionale de Santé
(ARS) to target monitoring and control
Modèle
Observation
PhD Marion HarambourgFirst results of integrating SIT to the model
200,000 sterile males released
weekly for 3 months
With a constant climate, return
to equilibrium only 1.5 month
after releases
Perspectives
Boosted SIT
Study various release scenarios
Impact of Climate on vector control
PhD Marion HarambourgMass rearing and irradiation
procedures
35Sex separation method (female elimination) and
quality control
Sexing of mosquitoes and handling Quality control of produced strains
procedures
2 genetic strategies to be compared to
Tragsa Laser sorter:
‐ Development of non‐transgenic GSS
‐ RNAi sexing
36
PhD students to be interviewed tomorrowDevelopment of an automatic release machine
Photo WeRobotics
Successful trial of the drone release system in Brazil on Aedes aegypti
in March 2018
37Regulatory issues
and social acceptability of boosted SIT
Risk assessment Regulatory issues Social acceptability
Review of regulatory issues, technical constraints and
social acceptability of genetic control in Europe
38Revolinc & regulatory issues
1 expert / 2 audited experts
39Revolinc & social sciences
• La Reunion island
• 29 jan ‐ 16 feb 2018
• Survey by observations
• Meeting of scientific actors
from CIRAD, Revolinc, IRD,
TIS‐Réunion, ARS and
collaborators on CYROI
platform of Saint‐Denis in the
context of phase 2
preparation meeting of TIS‐
project
PhD Kamil GhouatiRevolinc & social sciences
• Vienna, Seibersdorf
(Austria)
• 26 ‐ 29 mar. 2018
• Survey by interviews &
observations
• Meeting of members from
Insect Pest Control Lab (
FAO / IAEA Programme on
Nuclear Techniques in
Food and Agriculture )
PhD Kamil GhouatiRevolinc & social sciences
• Vienna, Seibersdorf (Austria)
• 23 ‐ 30 april 2018
• Survey by interviews &
observations
• Meeting with european
stakeholders in charge of
mosquito control during a
meeting on stakeholder
engagement in mosquito SIT
projects organized by IAEA
PhD Kamil GhouatiRevolinc & social sciences
Preliminary results
1. Identification of actors in the different sites: project
leaders, stakeholders, collaborators, funding agencies,
civil society, public(s), beneficiaries…
2. Identification of actors logics, categories involved to
account for the action & social dimension of SIT projects,
on scientific & technical thematics in the society, on
technical democracy and on research participation:
education, sensitization, communication, vulgarization,
etc.
3. Study of social relationships and their dynamics along
with ongoing projects deployment
PhD Kamil GhouatiThanks!
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