Nanotechnology Test Results of Bacterial Growth on Surfaces after using Zoono at Arlington Heights Veterinary Clinic - ZoonoUSA.com
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Nanotechnology Test Results of Bacterial
Growth on Surfaces after using Zoono® at
Arlington Heights Veterinary Clinic
Pinpoint Resolutions, LLC / Arlington Heights Veterinary Clinic
The following is the summary of a 30-day then an application of the protectant creates
study conducted at Arlington Heights a mechanical barrier providing an
Veterinary Clinic in Bloomington, Indiana antimicrobial environment that cannot
from August through September, 2019. diminish in strength, cannot leave the surface
to which it is applied and cannot allow the
BACKGROUND: organisms that it targets to acclimate. It’s a
stealth solution used in protecting the pets
In discussions with various Veterinarians, it is and people visiting and working at the clinic.
always a regimented course of action to keep
the Veterinary Clinic free of germs and viruses
due to the very nature of the patient. TESTING THE VETERINARY MEDICAL
ENVIRONMENT:
It’s difficult to ask the 185-pound St. Bernard
to stop drooling as he meets another friend Dr. Miller, being the scientist first and the
and explores new areas of the waiting room. compassionate Veterinarian second, wanted
Or the shy, colorfully patterned Munchkin Cat to test the products making sure this was the
to stop sneezing while she allows her best solution. The test was a routine treated
temperature to be taken in the exam room. vs. control environmental, and was measured
All the best sanitizing and cleaning was done, in the number of colonies formed with:
but efforts were a continual practice with the [Low] representing under 1000 colonies;
volume of pets coming to the office for care [Medium] representing 1001–10,000
and comfort. colonies; and
[High] representing over 10,001
Dr. Miller was in search of more practical colonies.
solutions that were being done in other areas
of healthcare that could be adopted to help The results would allow Dr. Miller to measure
the furry extended family. the ability of Zoono® to preserve the sterile
state of surfaces in the Veterinary Clinic as
In speaking with Dr. Miller, Pinpoint compared to normal use conditions. If the
Resolutions LLC introduced the Zoono® Zoono® technology in the most highly
technology that represented a new science trafficked areas of the clinic, can deliver a
named Nanotechnology. With the various meaningfully quantitative benefit as
forms of application, this active microbial compared to current sanitation best practices,
barrier would soon be the exact solution. then Arlington Heights Veterinary Clinic will
utilize this technology throughout the entire
It begins with a simple and safe disinfectant clinic.
based on the chemistry of chlorine dioxide,
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©2019 - All Results are Property of Arlington Heights Veterinary Hospital and Pinpoint Resolutions, LLC
TEST HYPOTHESIS: likely and the true results from the Zoono®
product verses the non-treated area can be
Treatment of hard surfaces with Zoono® will studied in a controlled environment.
result in a measurable sustained hygienic
environmental state as compared to surfaces After application of the Zoono® product, the
under similar conditions, not treated with vertical surface (where the testing is taking
Zoono®. place), will be cultured at one-week intervals
for one month / four weeks. (See Graphs 1-
TESTING PROTOCOL: 4)
The procedure for testing the Zoono® product The culture process will be to use swab and
is to culture two areas within the same culturette. This will then be sent to an
vicinity, in the Veterinary Clinic lobby. This independent outside laboratory to be
will be a vertical surface near the reception evaluated. The laboratory can perform
counter where coughing dogs and sneezing quantity of colonies found and identity of the
cats have the highest exposure. The lobby bacteria.
was chosen as the location to test due to the
high rate of exposure to sick patients. RESULTS SUMMARY:
Most concerns from owners over nosocomial In Table 1 shows the summary of the results
infection, arise from contact with pathogens of each vertical surface. These results
in the lobby, making this an ideal testing area reflected that within the untreated area that
and consequently a strong selling point when bacteria level increased as the cumulative
determining if the product works well. number of days and hours amplified and the
treated area experienced no bacterial growth
The procedure will start with culturing both until one type Sphingobacterium appeared.
surfaces before any products are applied. The This microorganism is ubiquitous in nature
next step is to clean both surfaces with a but is rarely involved in human infections.
quaternary ammonium disinfectant and then Sphingobacterium can be found in soil, on
apply the Zoono® product to one of the two plants, in foodstuffs and in water sources.
areas. The spray will be used instead of the Sphingobacterium species have the ability to
fogger so that cross contamination is less survive in moist hospital environments ₁.
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©2019 - All Results are Property of Arlington Heights Veterinary Hospital and Pinpoint Resolutions, LLCTABLE 1
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©2019 - All Results are Property of Arlington Heights Veterinary Hospital and Pinpoint Resolutions, LLCGRAPH 1
First Bacteriological Analysis - 336 Hours
Sphingobacterium
Pseudomonas Oryzihabitans
Pantoea sp.
Microbacterium sp.
Gram Positive Coccus
Stenotrophomonas maltophilia
Enterococcus sp.
0 0.5 1 1.5 2 2.5 3 3.5
Treated Untreated
GRAPH 2
Second Bacteriological Analysis - 528 Hours
Sphingobacterium
Pseudomonas Oryzihabitans
Pantoea sp.
Microbacterium sp.
Gram Positive Coccus
Stenotrophomonas maltophilia
Enterococcus sp.
0 0.5 1 1.5 2 2.5 3 3.5
Treated Untreated
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©2019 - All Results are Property of Arlington Heights Veterinary Hospital and Pinpoint Resolutions, LLCGRAPH 3
GRAPH 4
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©2019 - All Results are Property of Arlington Heights Veterinary Hospital and Pinpoint Resolutions, LLCCONCLUSION: Surface and environmental hygiene is an
important piece in maintaining a healthy,
The performance of Zoono® in this study sanitary environment. However, as the
showed that not only would the addition of CDC reports hands are the number one
Zoono® products such as Microbe Shield vector of germ transmission with 80% of
improve the overall level of sanitation in germs that make people sick are
treated areas, the product shows that attributable to hand hygiene. Healthy
bacteria accumulation and formation are hands combined with sanitary surfaces is
reduced, and consequently contamination is the best combination of practices to break
not able to begin. As the continued use of the cycle of infection and ensure healthy
Zoono® products to include, disinfectant and populations, in particular employees so as
then the application of the protectant over to mitigate illness related absenteeism.
time, there will be a hygienically improved
state in the treated environments. “As one Veterinarian to another, I was
amazed with the credibility of the Zoono®
Lastly, the daily cleansing with harmful product and learned the full technology found
disinfectants is not good for constant use. in Nanotechnology. Since my patients can’t
There are some disinfectant manufacturers wash their paws easily, I and my wonderful
that make the contemptible claim that their staff have to protect our patients. By
product will “kill ALL germs and viruses” to completing a 30-day hygienic process to
protect your family. Unfortunately, not all include Zoono® has given me a level of
disinfectants are effective against every type confidence, that I am doing no harm while
of bacteria or bug and while you may think increasing my patients’ and staff safety.”
these products are keeping you healthy and
safe; the actuality is that some of them may Dr. Robert Dale Miller II, DVM -
be harmful to both your health and the Arlington Heights Veterinary Hospital
environment.2
Allow the mechanical properties of
Nanotechnology destroy the harmful bacteria
and create a once-a-month process as the
evidence throughout this paper has
demonstrated.
₁.
Sphingobacterium multivorum: case report
RECOMMENDATIONS:
and literature review; F. Barahona and J.
To improve environmental hygiene of the
Slim; New Microbes New Infect. 2015 Sep;
facility, it is recommended that a broader
7: 33–36.
deployment of Zoono® be considered. This
additional deployment may begin as a 2.
strategic targeting of presumed high Disinfectants: Bad for Microbes, Bad for
contamination areas that may include the You: TUFTS ENVIRONMENTAL HEALTH AND
Clinic surgery area, Recovery or PACU, SAFETY; VOLUME VIII, ISSUE 3; Page 9.
Kennel, Restrooms, Office Waiting Area,
Exam Rooms etc.
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