Laboratory Tests for Allergy - Melbourne Pathology

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Laboratory Tests for Allergy - Melbourne Pathology
Laboratory Tests for Allergy
Laboratory Tests for Allergy - Melbourne Pathology
Allergen Specific IgE - RAST Testing                                                                                                                      Billing Policy

                                                                                                                For example if you request:
                                       Our fees for different allergen types
                                        Single                         $5                                       Three single allergens @ $5                          $15
                                        Mixed                          $10                                      Two mixed allergens @ $10                            $20
                                        Allergen Components            $40                                      One allergen component @ $40                         $40

                                       Billing policy for allergy testing                                       Your patient will receive an invoice for $100 (which
                                                                                                                includes our allergen test fee of $25).
                                       Our policy is to respect your request for the allergens
                                       and decode them according to our best practice. Even                     Practically, this means we will not accept a bulk-billed
                                       if you have requested more allergens than our allowance                  request for more than four single allergens, two
                                       under Medicare, we will bill your patient according to                   mixed allergens, or allergen components.
                                       the pricing using the key above. If you have nominated                   Please note that eligible patients may be able to
                                       the patient to be bulk-billed or if the patient has signed               claim a rebate on four occasions per year for testing
                                       a Medicare assignment form, this billing policy will still               performed on four separate blood collections, with
                                       apply for all tests above our allowance under Medicare.                  each episode at least 14 days apart.
                                       You will see in our Allergen Menu that we now list a                     Ordering allergy tests
                                       price for each allergen reagent and that this is organised
                                                                                                                We ask that you specifically nominate which allergens you
                                       into different allergen classes: single allergens are $5,
                                                                                                                would like tested. Please do not write the allergens to be
                                       mixed allergens are $10. Our highly specialised allergen
                                                                                                                tested in the clinical notes section of the request form.
                                       components are $40.
                                                                                                                A comprehensive menu of allergens that we test for is
                                       If the price of the allergens requested is more than the                 available in this brochure. If allergens are not available we
                                       Medicare rebate of $22.80, the laboratory will not accept                may use cross-reactive ones or advise you on the report.
                                       bulk-billing for the request and the patient will be billed
                                                                                                                Some “allergy symptoms” may result from intolerance
                                       according to the price on the menu with our additional
                                                                                                                mechanisms (salicylates, amines, MSG, metabisulphite)
                                       allergen test fee of $25 and may claim a rebate of
                                                                                                                and detection of IgE to them is not useful or possible. You
                                       $22.80, subject to coning.
                                                                                                                can request the allergens using their alphanumeric codes.
                                                                                                                To assist staff performing data entry, we would appreciate
                                                                                                                if you precede these codes by “Specific IgE for” or
                                                                                                                “Allergy serology for” or “RAST for”.

                                       Initial Investigation Panels                                      A1: CHILD 6 years or less                  A2: ADULT, or CHILD over 6 years
                                       If you write “RAST” or “Allergy serology”, but do                 Total IgE                                  Total IgE
                                       not specify the allergens, we will perform the tests              F2     Cow’s milk                          M6        Alternaria

                                       listed under A1 (for a child 6 years or less) or A2               D1     D. Pteronyssinus (Dustmite)         E1        Cat
                                       (Adult, or child over 6 years).                                   F1     Egg white                           E5        Dog
                                                                                                         F13    Peanut                              D1        D. Pteronyssinus (Dustmite)
                                       Any subsequently requested additional allergens
                                                                                                         F14    Soy                                 G5        Grass Pollen
                                       are charged according to the billing policy outlined                                                                   (Perennial Rye grass)
                                       above. For panels A1, A2 or A3 we will accept the
                                       Medicare rebate.

                                       A3: FOOD & INHALANTS
                                       FX5 Staple Foods (egg white,         G5   Grass Pollen                  D1     D. Pteronyssinus (Dustmite)        M6    Alternaria
                                            cow's milk, peanut, soy,             (Perennial Rye grass)
                                            wheat, and codfish)
                                                                                                                                                                                            3
Laboratory Tests for Allergy - Melbourne Pathology
Background and Testing Strategies

                                                                                                                                      Diagnostic use of sIgE testing                                     As with other tests in pathology, diagnostic accuracy is
      ƒƒ T
          esting for IgE to specific             ƒƒ T
                                                      his information provides a          ƒƒ D
                                                                                               ue to Medicare funding                                                                                   not 100 percent, ie. the presence of sIgE to a particular
         allergens (“RAST” testing) is an            summary of how this testing can          constraints, the laboratory is          sIgE is usually measured to confirm an allergic aetiology
                                                                                                                                      for symptoms when there is a history that suggests a               allergen is not 100 percent diagnostic of clinical reactivity
         important step in the diagnosis             be best utilised and outlines the        unable to accept bulk billing                                                                              to that allergen and absence of sIgE to a particular
         of allergy                                  cost of testing                          requests for more than very             possible allergic cause. sIgE measurements are used in
                                                                                                                                      the diagnostic algorithm of Type I allergy as presented in         allergen does not 100 percent exclude clinical reactivity
                                                                                              basic allergy testing requests
                                                                                                                                      Figure 2.                                                          to that allergen.
                                                                                                                                      Testing for sIgE is not affected by medications.                   While the level of sIgE correlates with the likelihood of
    Background                                                         “RAST” tests, which due to new laboratory techniques,          Interpretation of sIgE testing is very dependent on the            allergy, it does not correlate with the severity of an allergic
    Immediate (Type I) hypersensitivity refers to a specific type      are more accurately referred to as specific IgE (or) sIgE      clinical picture.                                                  reaction. ie. severe reactions may occur in those with low
    of immune response in which Immunoglobulin E (IgE)                 tests, detect the presence of specific IgE in the serum                                                                           level sIgE and vice versa.
    plays a central role. Clinical manifestations of immediate         and were introduced into clinical practice in the early
    hypersensitivity most commonly involve the skin                    1980’s. Techniques for detection of allergen-specific IgE
                                                                                                                                                                                      History and examination
    (urticaria), eyes (conjunctivitis), respiratory tract (rhinitis    have improved significantly since this time.
    and asthma) and cardiovascular system (hypotension).               Melbourne Pathology has introduced a new platform
    IgE is produced after first exposure to an allergen                on which sIgE testing is performed – the Phadia
    (an environmental molecule that induces an allergic                ImmunoCap 250. As the majority of published studies in
    response). This IgE subsequently binds to mast cells in            allergy use this testing method, it is widely considered the
                                                                                                                                                                         Suggest Type I allergy                                             Doesn’t suggest Type I
    tissues, such as those mentioned above. Subsequent                 benchmark for quantifying sIgE.
                                                                                                                                                                                                                                                    allergy
    exposure to allergen causes activation of the mast cells,
    to which the allergen-specific IgE is bound, causing
    release of chemicals that cause the clinical manifestations
    of allergy (see Figure 1 below).
                                                                                                                                                     sIgE positive                                    sIgE negative                            sIgE testing is not
                                                                                                                                                                                                                                                   indicated*

           First Exposure to Allergen                                                                                                                                                                                                    *Please note: If sIgE testing
                                                                                                                                                                                                                                         has been done inadvertently
                                                                                                                                                  Allergy confirmed                                Allergy not excluded                  in this situation and is positive,
                                                                                                                                                                                                                                         consider referral to an allergist.

                                                                                 IgE                                                    Avoidance or consider desensitisation              Referral to allergist, if IgE mediated
                                                                              production                                                     (aeroallergens and stinging                  pathology is considered highly likely
            Allergen                                         B cell                                                                                 insect allergy)                    clinically, or if the risks of a false negative
                                                                                                     IgE attachment                                                                     result are high (eg. Allergen associated
                                                                                                       to mast cell                                                                                   with anaphylaxis)
           Second Exposure to Allergen                                                                                                Figure 2

                                                             Exposure to
                                                               allergen

                 Mast cell with                                                                Allergen attachment and
             allergen-specific IgE                                                            degranulation of mast cell

     Figure 1

4                                                                                                                                                                                                                                                                             5
Allergens for which sIgE can be measured                                                                                          Clinical vignettes                                               Conclusion
    Several hundred allergens are available for testing. Targeted testing after a good clinical history allows maximisation           1.    35 year old female presents with a history of
                                                                                                                                           A                                                           sIgE testing is very useful for confirmation of an allergic
    of predictive values of sIgE tests.                                                                                                    recurrent upper airway swelling following pesto. In         aetiology in the setting of a history that suggests a
                                                                                                                                           between episodes, she has tolerated pinenut. Pesto          specific allergic cause for symptoms.
      Allergens can be conveniently grouped according to Clinical Syndrome:                                                                often contains cashew nuts, so a cashew specific
                                                                                                                                           IgE is ordered and returns a result of 37 kIU/L.            Dr Gary Unglik
     FOOD                                                                                                                                  As she has tolerated other tree nuts in between             MBBS, FRACP, FRCPA
     Clinical manifestations of immediate hypersensitivity include one or more of acute urticaria, angioedema, wheeze                      episodes, she is told that she can continue to eat          Immunology
     or hypotension.                                                                                                                       them cautiously (with the knowledge that there is a
                                                                                                                                                                                                       After graduating from Monash University
         	Most common in children: Cow’s milk, egg, peanut, tree nuts (most commonly cashew nut), sesame seed,                           small risk of reacting to cross contaminating cashew)       in 1997, Dr Unglik trained at the Royal
            soy and wheat                                                                                                                  and supplied with an adrenaline auto-injector and           Melbourne and Alfred Hospitals.
          Most common in adults: Peanut, tree nuts, “shellfish” (especially crustacean), fish, seeds and egg                              anaphylaxis plan to improve her safety, should she          He obtained combined fellowship with
                                                                                                                                           have accidental exposure.                                   both the Royal Australasian College of
     AEROALLERGENS                                                                                                                                                                                     Physicians and the Royal College of
                                                                                                                                      2.    17 year old male with a history of allergic rhinitis
                                                                                                                                           A                                                           Pathologists of Australasia in 2007.
     Clinical manifestations include one or more of rhinitis, asthma and occasionally dermatitis.
                                                                                                                                           only manifest in the spring has specific IgE ordered
     Perennial                                                                                                                             for House Dust Mite (HDM), Cat, Dog and “Perennial          Dr Unglik joined Melbourne Pathology in February 2010 as a
          House dust mite, pet dander and less commonly moulds (eg. Penicillium/aspergillus) and cockroach                                                                                            Consultant Immunopathologist. He is also a Consultant Clinical
                                                                                                                                           Rye Grass” (a grass that only pollinates in the spring      Immunologist and Allergist in the Department of Clinical Immunology
     Seasonal                                                                                                                              in Victoria). HDM returns a result of 1.5 kIU/L,            and Allergy at the Royal Melbourne Hospital where he is also
        Spring: Perennial Rye grass/Bermuda grass                                                                                         “Perennial Rye Grass”, 3kIU/L with Cat and Dog              Consultant Immunopathologist in the Department of Pathology.
        Summer: Bermuda grass and moulds (eg. Cladosporium, Altenaria)                                                                    giving results of
AllergenMenu
                                                                                                             Allergen Menu                                                                                                                    Allergen Menu

       Single Allergens $5 each                                                                                                             Single Allergens $5 each
       Note: Requests for more than four of these will exceed our allowance under Medicare                                                  Note: Requests for more than four of these will exceed our allowance under Medicare

    ANIMAL & AVIAN PROTEINS                          Chymopapain                            C209   MOULDS, YEASTS & TOXINS               MITES (HOUSE DUST & STORAGE)                     PARASITES                                 VENOMS
    Budgerigar droppings              E77            Gelatin bovine                         C74    Alternaria alternata           M6     Acarus siro                       D70            Anisakis                           P4     Dolichovespula maculata          I2
                                                                                                                                                                                                                                    (White-faced hornet)
    Budgerigar feathers               E78            Insulin human                          C73    Aspergillus flavus             M228   Blomia Tropicalis                 D201           Ascaris                            P1
                                                                                                                                                                                                                                    Honey bee (Apis mellifera)       I1
    Canary bird feathers              E201           Morphine                               C260   Aspergillus fumigatus          M3     Dermatophagoides farinae          D2
                                                                                                                                                                                          TREE POLLENS                              Paper wasp (Polistes spp.)       I4
    Cat epithelium & dander           E1             Penicilloyl G                          C1     Aspergillus niger              M207   Dermatophagoides microceras       D3
                                                                                                                                                                                          Acacia                             T19    Polistes dominulus (European     I77
    Chicken droppings                 E218           Penicilloyl V                          C2     Aspergillus terreus            M36    Dermatophagoides pteronyssinus    D1
                                                                                                                                                                                                                                    paper wasp)
    Chicken feathers                  E85                                                                                                                                                 American beech                     T5
                                                     Pholcodine                             C261   Aureobasidium pullulans        M12    Euroglyphus maynei                D74
                                                                                                                                                                                                                                    Vespa crabro (European Hornet)   I75
    Chicken serum proteins            E219                                                                                                                                                Australian pine                    T73
                                                     Suxamethonium (Succinylcholine)        C202   Botrytis cinerea               M7     Glycyphagus domesticus            D73
                                                                                                                                                                                                                                    Yellow hornet (Dolichovespula    I5
    Cow dander                        E4                                                                                                                                                  Birch                              T3     arenaria)
                                                     Tetanus toxoid                         C208   Candida albicans (yeast)       M5     House dust                        H2
    Dog dander                        E5                                                                                                                                                  Box-elder                          T1     Yellow jacket (Vespula spp.      I3
                                                                                                   Cephalosporium acremonium      M202   Lepidoglyphus destructor          D71
                                                     GRASS & GRAIN POLLENS                                                                                                                Chestnut                           T206   Common wasp)
    Duck feathers                     E86                                                          Chaetomium globosum            M208   Tyrophagus putrescentiae          D72
                                                     Bahia grass                            G17                                                                                           Cottonwood                         T14
    Finch feathers                    E214                                                         Cladosporium herbarum          M2
                                                     Barley grain                           G201                                                                                          Cypress                            T222
    Goat epithelium                   E80                                                          Curvularia lunata              M16
                                                     Bermuda grass                          G2                                                                                            Date                               T214
    Goose feathers                    E70                                                          Epicoccum purpurascens         M14
                                                     Brome grass                            G11                                                                                           Elm                                T8
    Guinea pig epithelium             E6                                                           Fusarium moniliforme           M9
                                                     Cultivated oat                         G14                                                                                           Eucalyptus                         T18
    Horse dander                      E3                                                           Helminthosporium halodes       M8
                                                     Cultivated wheat                       G15                                                                                           Grey alder                         T2
    Mouse epithelium                  E71                                                          Malassezia spp.                M227
                                                     Grass pollen (Fescue)                  G4                                                                                            Italian cypress                    T23
    Mouse serum proteins              E76                                                          Mucor racemosus                M4
                                                     Johnson grass                          G10                                                                                           Japanese cedar                     T17
    Mouse urine proteins              E72                                                          Penicillium glabrum            M209
                                                     Meadow grass                           G8                                                                                            Melaleuca                          T21
    Parakeet droppings                E197                                                         Penicillium notatum            M1     MISCELLANEOUS
                                                     Perennial Rye grass                    G5                                                                                            Mountain juniper                   T6
    Parakeet feathers                 E196                                                         Phoma betae                    M13    Cotton crude fibres               O1
                                                     Sweet vernal grass                     G1                                                                                            Oak                                T7     WEED AND CROP POLLENS
    Parrot feathers                   E213                                                         Rhizopus nigricans             M11    Seminal fluid                     O70
                                                     Timothy grass                          G6                                                                                            Oil palm                           T223   Canola (Rapeseed)                W203
    Pigeon droppings                  E7                                                           Staphylococcus enterotoxin A   M80    Tetramin fish feed                O203
                                                     Velvet grass                           G13                                                                                           Olive                              T9     Careless weed                    z
    Pigeon feathers                   E215                                                         Stemphylium botryosum          M10    Tobacco leaf                      O201
    Rabbit epithelium                 E82                                                                                                                                                 Peppertree                         T217   Common pigweed                   W14
                                                                                                   Tilletia tritici               M201
                                                     INSECTS
    Rabbit serum proteins             E206                                                                                               OCCUPATIONALS                                    Pine                               T213   Common ragweed                   W1
                                                                                                   Trichoderma viride             M15
                                                     Berlin beetle                          I76
    Rabbit urine proteins             E211                                                                                               Alphagal                          K048           Privet pollen                      T210   Dandelion                        W8
                                                                                                   Trichophyton ment. var.        M211
                                                     Blood worm                             I73    interdigitale
    Rat epithelium                    E73                                                                                                Castor bean                       K71            Red cedar                          T57    English plantain                 W9
                                                     Cockroach American                     I206   Trichophyton rubrum            M205
    Rat serum proteins                E75                                                                                                Chloramin T                       K85            Sweet gum                          T211   False ragweed                    W4
                                                     Cockroach Oriental                     I207   Trichosporon pullulans         M203
    Rat urine proteins                E74                                                                                                Ethylene oxide                    K78            Sycamore, London plane             T11    Goosefoot Lamb`s quarters        W10
                                                     Cockroach (Blatella germanica)         I6     Ulocladium chartarum           M204
    Sheep epithelium                  E81                                                                                                Formaldehyde/Formalin             K80            White ash                          T15    Lupin                            W207
                                                     Fire ant (Solenopsis invicta )         I70
    Swine epithelium                  E83                                                                                                Green coffee bean                 K70            White pine                         T16    Mugwort                          W6
                                                     Green nimitti (Cladotanytarsus)        I72
    Swine serum albumin               E222                                                                                               Isocyanate HDI                    K77            Willow                             T12    Ox-eye daisy                     W7
                                                     Grain weevil (Sitophilius granarius)   I202
    Turkey feathers                   E89                                                                                                Isocyanate MDI                    K76                                                      Parietaria judaica               W21
                                                     Horse fly                              I204
                                                                                                                                         Isocyanate TDI                    K75                                                      Rough marshelder                 W16
                                                     Mediterranean Flour Moth               I203
    DRUGS                                                                                                                                Ispaghula                         K72                                                      Saltwort Russian thistle         W11
                                                     Mosquito spp (Aedes communis)          I71
    Amoxicilloyl                      C6                                                                                                 Latex, Hevea braziliensis         K82                                                      Sheep sorrel                     W18
                                                     Moth                                   I8
    Ampicilloyl                       C5                                                                                                 Silk                              K74                                                      Sunflower                        W204
    Cefaclor                          C7                                                                                                 Silk waste                        K73                                                      Western ragweed                  W2
    Chlorhexidine                     C8                                                                                                 Sunflower seed                    K84                                                      Wormwood                         W5
8                                                                                                                                        Trimellitic Anhydride TMA         K86                                                                                              9
Allergen Menu                                                                                                             Allergen Menu

           Single Allergens $5 each                                                                                                   Single Allergens $5 each
           Note: Requests for more than four of these will exceed our allowance under Medicare                                        Note: Requests for more than four of these will exceed our allowance under Medicare

     FRUIT & VEGETABLES                                  Orange                             F33    Beans - Soya          F14    SPICES                                              Mackerel                           F206   MISCELLANEOUS
     Apple                                F49            Papaya                             F293   Brazil nut            F18    Anise                                F271           Octopus                            F59    Cacao                           F93
     Apricot                              F237           Passionfruit                       F294   Buckwheat             F11    Basil                                F269           Oyster                             F290   Carob (E410)                    F296
     Asparagus                            F261           Peach                              F95    Canola                F316   Bay leaf                             F278           Red Snapper                        F381   Cochineal (Carmine red, E120)   F340
     Aubergine (eggplant)                 F262           Pear                               F94    Cashew nut            F202   Black pepper                         F280           Salmon                             F41    Coffee                          F221
     Avocado                              F96            Persimmon                          F301   Chickpea              F309   Caraway                              F265           Sardine (Japanese Pilchard)        F61    Guar, guar gum (E412)           F246
     Bamboo shoot                         F51            Pineapple                          F210   Coconut               F36    Cardamon                             F267           Scallop                            F338   Gum arabic (E414)               F297
     Banana                               F92            Plum                               F255   Common millet         F55    Chilli pepper                        F279           Shrimp                             F24    Honey                           F247
     Beetroot                             F319           Potato                             F35    Corn                  F8     Cinnamon                             F220           Snail                              F314   Hop (fruit cone)                F324
     Blackberry                           F211           Pumpkin                            F225   Fenugreek             F305   Coriander                            F317           Sole                               F337   Malt                            F90
     Blueberry                            F288           Raspberry                          F343   Gluten                F79    Curry (Santa Maria)                  F281           Squid                              F258   Mushroom (champignon)           F212
     Broccoli                             F260           Red currant                        F322   Hazel nut             F17    Fennel seed                          F219           Swordfish                          F312   Tea                             F222
     Brussel sprouts                      F217           Rockmelons                         F87    Japanese millet       F57    Ginger                               F270           Trout                              F204   Tragacanth (E413)               F298
     Cabbage                              F216           Rose hip                           F330   Lentil                F235   Green pepper (unripe seed)           F263           Tuna                               F40    Yeast (S. cerevisiae)           F45
     Carrot                               F31            Spinach                            F214   Linseed               F333   Mint                                 F332           Whitefish                          F384
     Cauliflower                          F291           Strawberry                         F44    Lupin                 F335   Mustard                              F89
     Celery                               F85            Sweet potato                       F54    Macadamia nut         F345   Oregano                              F283
     Cherry                               F242           Tomato                             F25    Oat                   F7     Paprika sweet pepper                 F218
     Cucumber                             F244           Watermelon                         F329   Pea                   F12    Parsley                              F86
     Date                                 F289                                                     Peanut                F13    Sage                                 F344
                                                         MEAT
     Fennel (fresh)                       F276                                                                                  Thyme                                F273
                                                         Beef                               F27
     Fig                                  F328                                                                                  Vanilla                              F234
                                                         Chicken meat                       F83
     Garlic                               F47
                                                         Mutton                             F88                                 FISH & SHELLFISH
     Grape                                F259
                                                         Pork                               F26                                 Abalone                              F346
     Grapefruit                           F209
                                                         Rabbit meat                        F213                                Anchovy                              F313           MILK
     Guava                                F292
                                                         Turkey meat                        F284   Pecan nut             F201   Blue mussel                          F37            Alpha lactalbumin                  F76
     Kiwi fruit                           F84
                                                                                                   Pine nut (pignoles)   F253   Catfish                              F369           Beta lactoglobulin                 F77
                                                         POULTRY
                                                                                                   Pistachio             F203   Clam                                 F207           Casein                             F78
                                                         Chicken meat                       F83
                                                                                                   Poppy seed            F224   Crab                                 F23            Cheese Cheddar-type                F81
                                                         Egg white                          F1
                                                                                                   Pumpkin seed          F226   Crayfish                             F320           Cheese Mould-type                  F82
                                                         Egg yolk                           F75
                                                                                                   Quinoa                F347   Eel                                  F264           Cow’s whey                         F236
                                                         Turkey meat                        F284
     Lemon                                F208                                                     Rice                  F9     Codfish                              F3             Goat milk                          F300
     Lettuce                              F215                                                     Rye                   F5     Grouper                              F410           Milk                               F2
                                                         SEED, LEGUMES & NUTS
     Lime                                 F306                                                     Sesame seed           F10    Haddock                              F42            Sheep milk                         F325
                                                         Almond                             F20
     Lychee                               F348                                                     Spelt wheat           F124   Hake                                 F307           Sheep whey                         F326
                                                         Barley                             F6
     Mandarin                             F302                                                     Sweet Chestnut        F299   Halibut                              F303
                                                         Beans - green                      F315
     Mango fruit                          F91                                                      Walnut                F256   Herring                              F205
                                                         Beans - Lima                       F182
     Olive (black fresh)                  F342                                                     Wheat                 F4     Jack mackerel (Scad)                 F60
                                                         Beans - Red kidney                 F287
     Onion                                F48                                                      White bean            F15    Lobster                              F80
10                                                                                                                                                                                                                                                                   11
Allergen Menu                                                                                                                                          Allergen Menu

        Mixed Allergens $10 each                                                                                                                               Mixed Allergens $10 each
        Note: Requests for more than two of these will exceed our allowance under Medicare                                                                     Note: Requests for more than two of these will exceed our allowance under Medicare

     HOUSE DUST                                                                 Tree Mix 6      Box elder, Birch,    TX6         (T1 T3 T5 T7 T10)          COMBINATION INHALANT MIXES                                                FOOD MIXES
                                                                                                American Beech, Oak,
     Dust & Mite Mix   Dust and mite mix        HX2    (H2 D1 D2 I6)                            Walnut                                                      Inhalant 3       Bermuda grass,       RX3      (G2 G5 G17 W1 W9 W10)      Cereal Mix        Wheat, Oat, Maize,       FX3    (F4 F7 F8 F10 F11)
                                                                                                                                                                             Perennial Rye grass,                                                       Sesame seed,
                                                                                Tree Mix 7      Olive, Willow, White      TX7    (T9 T12 T16 T18 T19 T21)                    Bahia grass, Common                                                        Buckwheat
     ANIMAL DANDER MIXES                                                                        Pine, Eucalyptus,                                                            Ragweed, English
                                                                                                Acacia, Melaleuca                                                            Plantain, Goosefoot                                      Fruit Mix         Orange, Apple,           FX15   (F33 F49 F92 F95)
     Animal Mix 1      Cat dander, Horse        EX1    (E1 E3 E4 E5)                                                                                                         Lamb’s Quarters                                                            Banana, Peach
                       dander, Cow dander,                                      Tree Mix 8      Box elder, Birch,    TX8         (T1 T3 T4 T7 T11)
                       Dog dander                                                               Hazel, Oak, Sycamore                                        Inhalant 4       Sweet Vernals grass,   RX4    (G2 G5 G1 W1 W6 W9)        Meat Mix          Pork, Beef, Chicken      FX73   (F26 F27 F83)
                                                                                                                                                                             Bermuda grass,                                           Nut Mix           Peanut, Hazel nut,       FX1    (F13 F17 F18 F20 F36)
     Animal Mix 2      Cat dander, Dog          EX2    (E1 E5 E6 E87 E88)       Tree Mix 9      Grey Alder, Birch,        TX9    (T2 T3 T4 T7 T12)                           Perennial Rye grass,
                       dander, Guinea pig                                                       Hazel, Oak, Willow                                                                                                                                      Brazil nut, Almond,
                                                                                                                                                                             Common Ragweed,                                                            Coconut
                       epithelium, Rat,                                                                                                                                      Mugwort, English
                       Mouse                                                    Tree Mix 10     Grey Alder,               TX10   (T2 T3 T4 T15)
                                                                                                Birch,White Ash                                                              Plantain                                                 Seafood Mix       Fish, Shrimp, Blue       FX2    (F3 F24 F37 F40 F41)
     Animal Mix 3      Guinea pig epithelium, EX70     (E6 E82 E84 E87 E88)                                                                                                                                                                             mussel, Tuna, Salmon
                       Rabbit epithelium,                                                                                                                   OCCUPATIONAL MIXES                                                        Spice Mix 1       Tarragon, Marjoram,      FX70   (F272 F274 F273 F275)
                       Hamster epithelium,                                      WEED POLLEN MIXES                                                                                                                                                       Thyme, Lovage
                       Rat, Mouse                                                                                                                           Chemicals 1      Isocyanates (TDI,      PAX5   (K75 K76 K77 K79)
                                                                                Weed & Flower   Common ragweed,           WX5    (W1 W6 W7 W8 W12)                           MDI, HDI), Phthalic                                      Spice Mix 2       Caraway, Mace,           FX71   (F265 F266 F267 F268)
     Bird Mix          Budgerigar feathers,     EX72   (E78 E201 E196 E213      Mix             Mugwort, Ox-eye                                                              anhydride                                                                  Cardamon, Clove
                       Canary bird feathers,           E214)                                    Daisy, Dandelion,
                       Parakeet feathers,                                                       Golden rod                                                  Chemicals 2      Ethylene oxide,        PAX6   (K78 K79 K80 K85)          Spice Mix 3       Basil, Fennel seed,      FX72   (F269 F219 F270 F271)
                       Parrot feathers, Finch                                                                                                                                Phthalic anhydride,                                                        Ginger, Anise
                       feathers                                                 Weed Mix 1      Common ragweed,           WX1    (W1 W6 W9 W10 W11)                          Formaldehyde,
                                                                                                Mugwort, English                                                             Chloramin T                                              Staple Food Mix   Egg white, Milk,         FX5    (F1 F2 F3 F4 F13 F14)
     Feather Mix       Goose feathers,          EX71   (E70 E85 E86 E89)                        Plantain, Goosefoot                                                                                                                                     Fish, Wheat, Peanut,
                       Chicken feathers,                                                        Lamb’s Quarters,                                            Occupational 4   Wheat & Soy flour,     PAX4   (F4 F14 K87 I202)                            Soybean
                       Duck feathers, Turkey                                                    Saltwort                                                                     alpha-amylase,
                       feathers                                                                                                                                              Sitophilus granarius                                     Vegetable Mix     Carrot, Potato,          FX19   (F31 F35 F214 F244)
                                                                                Weed Mix 2      Western ragweed,          WX2    (W2 W6 W9 W10 W15)                                                                                                     Spinach, Cucumber
                                                                                                Mugwort, English
                                                                                                Plantain, Goosefoot                                                                                                                   Food Mix 7        Tomato, Yeast, Garlic,   FX7    (F25 F45 F47 F48 F85)
     GRASS POLLEN MIXES
                                                                                                Lamb’s Quarters,                                                                                                                                        Onion, Celery
     Grass Mix 1       Cocksfoot, Meadow     GX1       (G3 G4 G5 G6 G8)                         Scale Lenscale                                                                                                                        Food Mix 20       Wheat, Rye, Barley,      FX20   (F4 F5 F6 F9)
                       Fescue, Perennial Rye
                                                                                                                                                                                                                                                        Rice
                       grass, Timothy grass,
                       Meadow grass                                             MOULD MIXES                                                                                                                                           Food Mix 26       Egg white, Cow’s milk, FX26     (F1 F2 F13 F89)
                                                                                                                                                                                                                                                        Peanut, Mustard
     Grass Mix 2       Bermuda grass,           GX2    (G2 G5 G6 G8 G10 G17)    Mould Mix 1     Penicillium               MX1
                       Perennial Rye grass,                                                     chrysogenum,                                                                                                                          Food Mix 74       Cod, Herring,            FX74   (F3 F205 F206 F254)
                       Timothy grass,                                                           Cladosporium                                                                                                                                            Mackerel, Plaice
                       Meadow grass,                                                            herbarum, Aspergillus
                       Johnson grass, Bahia                                                     fumigatus, Alternaria
                       grass                                                                    alternata
     Grass Mix 4       Sweet Vernal grass,      GX4    (G1, G5, G7, G12, G13)   Mould Mix 2     Penicillium               MX2    (M1 M2 M3 M5 M6 M8)
                       Perennial Rye grass,                                                     chrysogenum,
                       Common reed,                                                             Cladosporium
                       Cultivated rye, Velvet                                                   herbarum, Aspergillus
                       grass                                                                    fumigatus,
                                                                                                Candida albicans,
                                                                                                Alternaria alternata,
     TREE POLLEN MIXES                                                                          Setomelanomma
                                                                                                rostrata
     Tree Mix 1        Box elder, Birch, Oak,   TX1    (T1 T3 T7 T8 T10)
                       Elm, Walnut                                              Mould Mix 4     A. fumigatus, A. niger,   MX4    (M3 M207 M36 M228)
                                                                                                A. terreus, A. flavus
     Tree Mix 2        Box elder, Oak, Elm,     TX2    (T1 T7 T8 T14 T22)
                       Cottonwood, Pecan
                       Hickory

     Tree Mix 3        Mountain Juniper,        TX3    (T6 T7 T8 T14 T20)
                       Oak, Elm,
                       Cottonwood,
                       Mesquite

     Tree Mix 4        Oak, Elm, Sycamore,      TX4    (T7 T8 T11 T12 T14)
                       Willow, Cotttonwood

     Tree Mix 5        Grey Alder, Hazel,       TX5    (T2 T4 T8 T12 T14)
                       Elm, Willow,
                       Cottonwood

12                                                                                                                                                                                                                                                                                                              13
Allergen Menu           Notes

        Allergen Components $40 each

     ANIMALS                                                               TREES
     rCan f1 (recombinant dog)                                      E101   rBet v 1 PR-10, Birch Betula verrucosa                T215
     rCan f2 (recombinant dog)                                      E102
                                                                           MOULDS
     rFel d1 (recombinant cat)                                      E94
                                                                           rAlt a 1 (component alternaria)                       M229
     nBos d 6 BSA, Cow Bos spp.                                     E204
                                                                           rAsp f 3 (recombinant aspergillus)                    M220
     nCan f 3 Dog serum albumin Canis familiaris                    E221
                                                                           rAsp f1 (recombinant aspergillus)                     M218
     nFel d 2 Cat serum albumin Felis domesticus                    E220
                                                                           rAsp f2 (recombinant aspergillus)                     M219
     FOODS                                                                 rAsp f4 (recombinant aspergillus)                     M221
     nBos d 4 a-lactalbumin, Milk Bos spp.                          F76    rAsp f6 (recombinant aspergillus)                     M222
     nBos d 5 b-lactoglobulin, Milk Bos spp.                        F77
                                                                           LATEX
     nBos d 8 Casein, Milk Bos spp.                                 F78
                                                                           rHev b 1 Latex Hevea brasiliensis                     K215
     nBos d Lactoferrin, Milk Bos spp.                              F334
                                                                           rHev b 3 Latex Hevea brasiliensis                     K217
     nGal d 1 Ovomucoid, Egg Gallus spp.                            F233
                                                                           rHev b 5 Latex Hevea brasiliensis                     K218
     nGal d 2 Ovalbumin, Egg Gallus spp.                            F232
                                                                           rHev b 6.01 Latex Hevea brasiliensis                  K219
     nGal d 3 Conalbumin, Egg Gallus spp.                           F323
                                                                           rHev b 6.02 Latex Hevea brasiliensis                  K220
     rAra h 1 Peanut Arachis hypogaea                               F422
                                                                           rHev b 8 Profilin, Latex Hevea brasiliensis           K221
     rAra h 2 Peanut Arachis hypogaea                               F423
                                                                           rHev b 9 Latex Hevea brasiliensis                     K222
     rAra h 3 Peanut Arachis hypogaea                               F424
                                                                           OCCUPATIONAL ALLERGENS
     rAra h 8 PR-10, Peanut Arachis hypogaea                        F352
                                                                           nCar p 1 Papain, Papaya Carica                        K201
     rAra h 9 LTP, Peanut Arachis hypogaea                          F427
                                                                           nAsp o 1 a-amylase Aspergillus oryzae                 K87
     rCor a 1 PR-10, Hazel nut Corylus avellana                     F428
     rCor a 8 LTP, Hazel nut Corylus avellana                       F425   OTHERS (to exclude CCD reactivity)
     rGad c 1 Cod Gadus morhua                                      F426   nAna c 2 Bromelin, Pineapple Ananas comosus           K202
     rPen a 1 Tropomyosin, Shrimp Penaeus aztecus                   F351   nO214 MUXF3 CCD, Bromelin                             O214
     rPru p 1 PR-10, Peach Prunus persica                           F419
                                                                           MISCELLANEOUS
     rPru p 3 LTP, Peach Prunus persica                             F420
                                                                           Alpha-gal (Gal-alpha-1,3-Gal Thyroglobulin, bovine)   U953
     rPru p 4 Profilin, Peach Prunus persica                        F421
     rTri a 19 Omega-5 Gliadin, Wheat Triticum spp                  F416
     Note: These allergens are priced as single allergens ($5 ea)

     VENOM
     rApi m 1 Phospholipase A2, Honey Bee                           I208
     rVes v 1 Phospholipase A1, Common Wasp                         I211
     rVes v 5 Common Wasp                                           I209
     rPol d 5 European Paper Wasp                                   I210

14                                                                                                                                              15
Head Office & Central Laboratory                 Regional Laboratories
103 Victoria Parade Collingwood, Victoria 3066   Ballarat
Switchboard                        9287 7700    63 Victoria Street 3350                              5331 6429
                                                 Bendigo
Facsimile                           9419 1486
                                                 First Floor, 1 Chum Street 3550                      5444 2382
Results                            9287 7777
                                                 Box Hill
Couriers                           9287 7788    Epworth Eastern Medical Centre
Helpdesk                           9287 7799    1 Arnold Street 3128                                 9899 3830

Accounts Department                9287 7888    Frankston
                                                 Frankston Private Hospital
www.mps.com.au                                   24–28 Frankston-Flinders Rd 3199                     9783 5582
                                                 Heidelberg
                                                 Suite 11, 210 Burgundy Street 3084                    9459 7404
                                                 Mornington
                                                 The Bays Hospital, Vale Street 3931                   5973 5811
                                                 Mulgrave
                                                 The Valley Private Hospital
                                                 Suite 4, 529 Police Road 3170                         9790 1388
                                                 Richmond
                                                 Epworth Centre
                                                 Suite 2.2, 32 Erin Street 3121                       9429 2222

                                                                         Details correct at time of printing – November 2013
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