Extending the Shelf-Life of RTE Meat Products - Lynn Knipe The Ohio State University

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Extending the Shelf-Life of
   RTE Meat Products
        Lynn
         y Knipe
              p
        The Ohio State
          U i
          University
                 it
Shelf Life Extension of
      RTE Meat
            M    P
                 Products
                     d
z   F d Spoilage
    Food S il
     • oxidation of color and flavor
z   Safety
     • L. monocytogenes
     • C. perfringens
Food Spoilage
                 p    g
z   Unsaturated fats
     •OOxidation
          id ti catalyzed
                    t l dbby h
                             heat,
                                t light,
                                   li ht
      trace metals.
     • Color
         - metmyoglobin formation
     • Flavor
      – cardboard aroma
z   Warmed over flavor
     • lean meat products
Antioxidants
z   Mostly phenolic compounds
z   Traditional antioxidants
     • BHA,
       BHA BHT,
            BHT TBHQ,
                TBHQ propyl gallate
z   Chelating agents
     • Citric acid, phosphates
z   Cleaner label antioxidants
     • Spices,
       S i     fruit
               f it extracts
                      t   t
Procedures That Delay
     th Onset
     the O   t off Oxidation
                   O id ti
z   Surface
    S  f     b
             browningi
z   S
    Sauces  &gravies
            &       i
z   Vacuum tumbling
             tumbling, mixing,
                       mixing
     stuffing,
            g, etc.
Clean Label
           A ti id t
           Antioxidants
z   Plums, grape seed
    Pl                d extract,
                            t t
     pine bark extract,
     rosemary, sage, oregano,
     marjoram,
         j       thyme,
                 th     mace,
     allspice, cloves, etc.
Plum Products
z   Juice, dried - uncured RTE
     sausage & roastt bbeeff
z   Dried paste - sausage
z   Effective in p
                 pre-cooked and
     uncured products.
Grape Seed Extract
z   Added at 0.02-0.1%
z   No flavor issue
z   R t d microbial
    Retards     i   bi l growth
                             th off
     Listeria,, E. coli and
     Salmonella.
z   D k
    Darkens   meatt color
                        l
z   Gravinol-Super
    Gravinol   Super (Kikkoman)
Rosemary
                 y
z   Oleoresin
     • use at 0.02 to 0.10%,
         depending upon the fat
         content of the product.
     • Herbalox, Kalsec Inc.
z   Essential oil
Other Spices
              p
z   Oregano
    • Water-soluble extract
    • Oreganox WS, RAD
     Natural Technologies)
z   Sage
Food Safety
Food Safety
z   P t L th lit Treatments
    Post-Lethality T t   t
     • effective after post-lethality exposure to
          potential
             t ti l environmental
                        i        t l
          contamination
     • achieve a minimum of 1 log reduction of
          L. monocytogenes
     • Listericidal
z   Growth Inhibitors
     • inhibit growth of L.
                         L monocytogenes to
          not more than 2 logs of growth over
          the duration of the p
                              product’s shelf life
     • Listeriostatic
Traditional Processing
z   Freezing product – prevents
     growth of L.
                L mono.,
                  mono must be
     kept
       p frozen throughout
                      g
     distribution
z   Fermentation
     • High
       Hi h acid
              id content
                    t t (< pH
                            H 4.5)
                              4 5)
Traditional Processing
z   Sodium nitrite
     • contributes
          t ib t tot the
                     th overall
                             ll
         suppression
           pp         of L.
         monocytogenes growth
z   Product Composition
     • higher fat increases Listeria
         heat resistance
Antimicrobial Agents
z   To
    T preventt growth th off post-
                             postt-cook
                                      k
     contamination.
     co  ta      at o
z   Added to product during
     f
     formulation.
            l ti
z   Added to finished product
     before packaging.
z   Added to pkg. materials.
z   Approved list: USDA Directive
     7120.1
Antimicrobial Agents
z   Organic Acids
     • acetic,
          ti citric,
               it i lactic,
                     l ti malic
                            li
z   Salt of Organic Acids
     • lactates,, diacetates,, citrates,,
z   Bacteriocins
     • Nisin, pediocin
z   Clean Label Ingredients
Potential Antimicrobial
            Agents
z   Smoke
    S   k – liquid
            li id or natural
                       t   l
z   Spices
Organic Acids
z   Reduce
    R  d     the
             th pHH off products
                            d t
z   Inhibits growth and/or death
     of microorganisms
z   Dipping or spraying wieners,
     cooked
          k dhham and d bologna
                         b l
     slices in acetic acid, lactic
     acid, and benzoic acid
     solutions
        l ti    was listericidal.
                     li t i id l
Organic Acids
z   Surface application
     reduces
       d      needd for
                    f adding
                        ddi
     to formula
        formula.
z   Vacuum packaging
     distributes over surface.
                      surface
Salts of Organic Acids
z   4.8%
    4  8% sodium
             di   and/or
                     d/ potassium
                            t    i
      lactate and 0.25% sodium
      diacetate approved as
      antimicrobial agents in meat
      product formulation.
z   Synergistic effect of lactates
      and diacetates in inhibiting
      growth (bacteriostatic) of L.
      monocytogenes
               t       i meatt
                       in
      products.
Lactates and Diacetates
z    Effective when added to
     prod ct formulations,
     product  form lations butb t
     not as a dip.
z   2-3% sodium lactate without
     flavor problems.
z   >0.12% sodium diacetate
     can resultlt in
                  i vinegar
                     i      flavor.
                            fl
Uncured Products
z   Higher levels of sodium
     lactate addition needed.
                       needed
z   Flavorings may need to be
     added to mildly y flavored
     products, because of
     lactate flavor at higher
     levels.
     levels
Purac’s Opti.Form
     Listeria
      i    i Control
              C      lM
                      Model
                        d l
z   Determine amount of lactate
     andd diacetate
          di    t t based
                    b   d upon
     level of control,
              control and:
z   Finished pproduct salt and
     moisture contents
Sodium Lactate
z   Improves water-
              water-holding
     capacit and cooking
     capacity
     yields.
z   Enhances beef roast flavor
     and reduces fading of beef
     bologna.
Buffered Sodium Citrate
       andd Sodium
            S di     Di
                     Diacetate
                          t t
z   1% Ional-
        Ional-Plus inhibits L.
     monocytogenes
            cytogenes and C.
     perfringens
        fi        germination
                       i ti    &
     outgrowth during chilling
z   Add dry y to p
                 product.
z   Use with fresh or cooked
     products.
         d t
Bacteriocins
z   Polypeptides made by
     bacteria that inhibit other
     bacteria.
     bacteria
z   Nisin & pediocin are
     bacteriocidal to L. mono. &
                 +
     other Gram organisms
z   Nisin commercially available
Nisin
z   Can be added to formula,
     applied to surface, coating
     to the inside of sausage
     casings, and barrier films.
z   Less effective in higher
                        g    fat
     products.
Lauric Arginate
z   Bacteriocidal upon application
z   Limited bacteristatic effect.
                          effect
z   Apply
     pp y as a surface treatment
     • spray into pouch at packaging.
     • dip system
     • tumble
z   Max. use (USDA FSIS) - 200
     ppm (Protect-M,
          (Protect-M Purac)
     • not on label
Octanoic Acid
z   Bacteriocidal fatty acid
     • processing aid
                  aid, not labeled (US)
                                   (US).
z   400 ppm final product weight.
     • Octa-gon (EcoLab)
z   Sprayed
     p y into RTE p   package,
                            g ,
     prior to vacuum sealing (1-2
     logs) and heat shrinking
     (activates).
z   N fl
    No flavor problems.
                  bl
Cetylpyridinium
       Chl id (CPC)
       Chloride
z   Approved
    A      d ffor poultry
                     lt
     carcasses ((Cecure,, Safe
     Foods Corp.), not approved
     f RTE products.
     for         d t
z   Reduces Listeria
     monocytogenes on RTE
     products.
Spices
z   Garlic,
    G   li sage, & cloves
                       l    – inhibit
                              i hibit
          o o
     L. mono.
z   Gram+ more sensitive to
     effects
      ff t off spices.
                   i
z   Spice extracts less effective
     than actual spices.
z   Essential oils of oregano &
     garlic inhibit L.
                     L mono.
                        mono when
     added to edible films.
Spices
z   Levels of spices needed to
     preventt L.
              L monocytogenes
                         t
     in foods high in protein or
     fat are higher
               g    than would
     commonly be used in meat
     products.
         d t .
Smoked Products
z   Natural
      atural & liquid smoke
     inhibit growth of L.  L mono.
                             mono.
z   New
      e liquid
           qu d smoke
                 s o e extract
                           e t act
     (AM--3, without color,
     (AM
     Z
     Zesti
     Zesti/Kerry)
         ti/Kerry)
         ti/K
           /K     ) th
                    thatt is
                          i sprayedd
     onto RTE meat products,
     approved as a flavoring.
Clean Label Products
     f C
     for Controlling
               lli   Listeria
                      i    i
z   C lt
    Cultured
           d sugars
     • Verdad products (Purac)
         •   also contains vinegar
     • DURAfresh (Kerry Seasonings)
         • 0
           0.05-1%
             05 1% addition level
         • Labelled “cultured corn syrup
          solids”
Clean Label Products
     f C
     for Controlling
               lli   Listeria
                      i    i
z   L
    Lemon juice
          j i & vinegar
                 i
     • Surface application, pickles, or
         direct addition
     • MOstatin (WTI,
                 (WTI Inc.)
                       Inc )
Post-Packaging
       Post-
      H t/P t
      Heat/Pasteurization
                  i ti
z   Heat re
         re--applied to package
     surfaces to destroy  y anyy
     surface cross contam’n
                     contam’n..
z   Hot water (185º
               (185ºF/85
                     F/85ººC, 8 min.)
z   Steam (205º
           (205ºF/96
                 F/96ººC,
                       C 15 sec)
z   More effective than
     antimicrobial agents (USDA).
Post-Packaging Heat
     Post-
       Di d
       Disadvantages
                t
z   Difficult to heat all surfaces
z   Mayy adversely y affect p
                            package. g
z   Product changes with heat
     (color,
     (color texture
              texture, purge
                       purge, etc
                               etc.))
z   Expensive
z   Apply heat/moisture in ref.
     room (condensation,
            (    d       i    etc.))
Individually-Wrapped
P d t are Easiest
Products    E i t tot Post-
                      P t
  Package Pasteurize/Heat
Cook-in-the-Bag Processing
z   Listeria contamination is not
     likely to occur as long as
     package is not opened and
        p
     exposed    to the environment.
z   Listeria contamination could
     occur at the point that final
     user opens the th package.
                           k
Lynn Knipe
        Processed Meats
       E t
       Extension
             i Specialist
                 S  i li t
    Ohio State University
                        y
        (614)292-4877
      knipe 1@os ed
      knipe.1@osu.edu
www.ag.ohio-state.edu/~meatsci/
     g
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