NATIinFVG: NIPT FOR TRACEABLE AND COMPUTERIZED ANEUPLOIDIES IN FRIULI VENEZIA GIULIA LABORATORY TRACEABILITY - Speaker: Dott. Diego Boscarino AB ...

NATIinFVG: NIPT FOR TRACEABLE AND
COMPUTERIZED ANEUPLOIDIES IN FRIULI VENEZIA
GIULIA
LABORATORY TRACEABILITY

Final Event, Trieste 17th October 2019

Speaker: Dott. Diego Boscarino (AB ANALITICA SRL)

PRESENTATION OVERVIEW

COMPANY PROFILE
AB ANALITICA IN THE NATIinFVG PROJECT
TRACEABILITY
AUTOMATION

                    NATIinFVG Final Event, Trieste 17th October 2019

AB ANALITICA
     Advance Biomedicine

«We design and produce solutions
     for better diagnostics»

WHO IS AB ANALITICA
❖ Strong experience in the production of
  diagnostic kits and reagents

❖ Since 30 years scientists develop
  innovative test in the diagnostic field

❖ Provider of the best assistance,
  technical support and customers
  service

❖ Eco sustainable company

La nostra crescita
   AB ANALITICA is present in over 40 Countries
       with its trusted distributor network

Engineering Team

                          R&D Team

AB ANALITICA is fully
committed in matching
both talent and skills
of its researchers                         Technical assistance &
                                             Customer support

                         Production Team

DIAGNOSTIC APPLICATION FIELDS

Infectivology      Human Papillomavirus           STI         Hepatitis C virus

           Oncohematology         Thrombophilia         HLA

Technologies

REAL TIME PCR

     REVERSE LINE BLOT

        NEXT GENERATION SEQUENCING

SMART WORKFLOW                     INTERNAL PRODUCTION
Panel of RT-PCR kits with same     High      professional    and
thermal profile, mastermix         qualified production team
ready to use, IC and               for all AB ANALITICA kits
dUTP/UNG inside

EFFICIENT AUTOMATION               SOFTWARE DEVELOPMENT
Automated solution sample to       Ingeneering     team     for
results. Extraction, PCR set-up,   developement              of
amplification and                  interpretation software and
interpretation                     automation software

HIGHT SPECIFICITY and              QUALITY CERTIFIED
PERFORMANCE                        Certified UNI EN ISO 9001
30 years of experience in          and UNI EN ISO 13485 for all
develop of diagnostic kits         products potfolio

Research & Development Workflow

  customer       planning                input

   validation           check        developing

 Final product   transfer

                                output

AB ANALITICA IN THE NATIinFVG PROJECT

                  KNOWLEDGE ACQUISITION AND CONCEPTUAL DEFINITION
•   Analysis of transfer steps in the whole process (sample and related information)
•   Storage requirements (short-term and long-term)
•   Nucleic acid extraction requirements for NGS downstream analysis, traceability and automation
•   Library preparation requirements for traceability and automation

                                        ACHIEVEMENTS
                         • Project biobank
                         • Traceability system
                         • System for automatic library preparation

                                                        NATIinFVG Final Event, Trieste 17th October 2019

TRACEABILITY: BASE PLATFORM

   AB SCAN
    SINGLE                       BioBanker

                                    AB
                                  PRINTER
AB SCAN ARRAY

                   NATIinFVG Final Event, Trieste 17th October 2019

TRACEABILITY: BASE PLATFORM

                                                          AB ANALITICA GENEQUALITY
Ultra congelatori e/o Freezer                                         X 120
Rack per stoccaggio provette                              con sistemi di lettura barcode
                                                               1D e scansione 2D
                                     Sistema
                                   Monitoraggio
                                   Temperatura

                                                               Capper/Decapper
   Stampanti etichette manuale e
                                                               per tappi a vite e
         automatizzate
                                       Sistema                    pressione
                                   riconoscimento
                                     BIOMETRICO

                                                    NATIinFVG Final Event, Trieste 17th October 2019

TRACEABILITY: ANALYSIS

                                                  Visual workflow
Flow chart

                                                Data flow

                       NATIinFVG Final Event, Trieste 17th October 2019

TRACEABILITY: IMPLEMENTATION

  Exploitation of existing functionalities
• 2 progress status fields (DNA extraction and Library Preparation) + primary code tracking field
• DNA extraction phase: barcode check of primary code on printed label physically propagated
  through all the steps
• Library preparation phase: plate well coordinates replicated through the aliquote functionality

    Custom functionalities (designed for more general application)
•   Valorization of the tracking field at sample creation by linking to existing fields (software)
•   ID Code generator (software)
•   Plate pipetting masks with barcode identification (software + hardware)
•   Traceability of QIASymphony output rack to input tube carrier (software + hardware)

                                                         NATIinFVG Final Event, Trieste 17th October 2019

AUTOMATION: DECK LAYOUT

               NATIinFVG Final Event, Trieste 17th October 2019

AUTOMATION: PROGRAMMING

              NATIinFVG Final Event, Trieste 17th October 2019