Chloroquine/ hydroxychloroquine prevention of coronavirus disease (COVID-19) in the healthcare setting; a randomised, placebo-controlled ...
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Chloroquine/ hydroxychloroquine prevention of coronavirus disease
(COVID-19) in the healthcare setting; a randomised, placebo-
controlled prophylaxis study
UK Sample Management v3.0, 2nd July 2020
Laboratory Testing & Sample
Processing
Clotted Capillary TNS
EDTA (Throat and Sputum*
Blood blood
Nose Swab)
Plasma Serum PK-DBS
Cell pellet
PK-DBS * At this time we are not collecting
sputum. You will be informed in
future if this situation changes.
V3.0, 02 July 2020 2
Work-Flow
V3.0, 02 July 2020 3
Work-Flow (Cont.)
Onset of Covid-19 or ARI
V3.0, 02 July 2020 4
Sample Management:
This is a screenshot of the sample management system we are using,
OpenSpecimen.
You will be granted access to this system. It allows us to track the location and
number of samples, as well as the dates of collection and other essential data.
If you have any specific queries about this system that aren’t covered in the training
manual then please contact the central trial team.
V3.0, 02 July 2020 5
Sample Management (Cont.)
When you start typing a participant’s ID in the top menu bar it will
allow you to click on the participant.
V3.0, 02 July 2020 6
Sample Management (Cont.)
This page provides access to each collection time point, and you just
press collect to start this process.
V3.0, 02 July 2020 7
Sample Management (Cont.)
This screen allows
you to fill in all
the necessary
information, as
well as complete
the blood
collection data
entry in
OpenSpecimen.
V3.0, 02 July 2020 8
Sample Management (Cont.)
This slide shows
how to
enter a
sample’s
location in
the storage box.
V3.0, 02 July 2020 9
Labelling
This is a picture of the labels you will be sent; they contain a round sticker to be placed
on the top of the tube and a rectangular one to be placed around the side.
These are pre-printed so all you need to do is affix them.
V3.0, 02 July 2020 10Plasma, cell pellet, serum processing
Invert 6-8
times before 3 vials
EDTA processing 0.5 mL plasma
(store
at 4°C) 1 vial
0.5 mL buffy
1,500 rcf, coat Immediate
10 minutes, Freeze
Serum 25 °C At < -80 °C
tube No brake
3 vials
(room
1 mL serum
temp for
30 mins)
V3.0, 02 July 2020 11DBS on 31 ET Chr paper for CQ level
Sample Label
100 µL
from EDTA
tube
BEFORE
spin (D0) • 1 DBS/Bag
Air dry
50 µL / spot 6 hr - o/n • Keep in cool ,
dry place
Finger • Attach circular
prick
No sticker to top
(D30, D60,
D90) hair right, below
dryer!! seal
V3.0, 02 July 2020 12Example for ‘Good’ and ‘Bad’ Quality DBS
V3.0, 02 July 2020 13SHIPMENTS
• 2 - 5 batches (depends on site facilities, number of samples, etc).
• Shipments will be pre-paid for with Davies International, and samples can be
transported 12 or 26 boxes at a time.
• When a collection is requested, a polystyrene box – already filled with dry ice – will
be sent to sites for arrival on a Tuesday. Sites will then fill the box, and it will be
collected on the same Tuesday for delivery to Oxford (DTU/OCDEM) on
Wednesday.
• Sample log data must be sent to the central trial team once shipment organized.
• Davies Int. will apply all the appropriate stickers to the boxes, eg UN3373, UN1845
etc.
V3.0, 02 July 2020 14Additional Information
Reference Documents:
• COPCOV UK Protocol
• COPCOV UK Lab Guidelines and SOP
Always refer to www.copcov.org/sites for correct version of
documents
If you have any further questions please contact copcov@dtu.ox.ac.uk
V3.0, 02 July 2020 15You can also read
