2nd ICA RESEARCH SYMPOSIUM 2019 |1 - UTM Pagoh
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2nd ICA RESEARCH SYMPOSIUM 2019 |2 CONTENTS WELCOME MESSAGES Deputy Vice-Chancellor (Research and Innovation), Universiti Teknologi Malaysia 3 Prof. Datuk TS. Dr. Ahmad Fauzi bin Ismail The Advisor of 2nd ICA Research Symposium, 4 Prof. Dr. Mohamad Roji bin Sarmidi The Chairman of 2nd ICA Research Symposium, 5 Dr. Cheng Kian Kai . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . PREFACE 6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . TENTATIVE PROGRAMME 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . SCIENTIFIC COMMITTEE MEMBERS 15 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ORGANIZING COMMITTEE MEMBERS 16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . BIOGRAPHY OF KEYNOTE AND INVITED SPEAKERS 19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ABSTRACTS Keynote 26 Oral Presentations 27 Poster Presentations 53 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . LIST OF SPONSORS 79 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . NOTES 81
2nd ICA RESEARCH SYMPOSIUM 2019 |3 MESSAGE FROM Deputy Vice-Chancellor (Research and Innovation) Universiti Teknologi Malaysia Prof. Datuk TS. Dr. Ahmad Fauzi bin Ismail Assalamualaikum WBT, I wish you all a hearty welcome to all participants of the 2nd Innovation Centre in Agritechnology for Advanced Bioprocessing (ICA) Research Symposium 2019. This symposium is an excellent opportunity for all participants from industry, university and agencies to interact with professionals in the field of agriculture and technology. I express my grateful thanks to the symposium organiser, collaborators, and contributors to make this event a success. PROFESSOR DATUK TS. DR. AHMAD FAUZI BIN ISMAIL Deputy Vice-Chancellor (Research and Innovation) Universiti Teknologi Malaysia
2nd ICA RESEARCH SYMPOSIUM 2019 |4 MESSAGE FROM The Advisor of 2nd ICA Research Symposium, Prof. Dr. Mohamad Roji bin Sarmidi Assalamualaikum WBT and Greetings everyone, It is my great pleasure to warmly welcome you to the 2nd Innovation Centre in Agritechnology for Advanced Bioprocessing (ICA) Research Symposium 2019. I also would like to express my highest appreciation to the organizing committee for their remarkable effort in making this conference a reality. It is a huge honor for me to sit under the same roof together with the best speakers, professors, experts, and researchers from all around the world to share and discuss about the issues and challenges arising in today’s world of agriculture. Let’s hope that this symposium can inspire new ideas, inventions, planning and discoveries for the betterment of agriculture. Finally, I wish all of you to have a successful and prosperous conference. Thank you. PROF. DR. MOHAMAD ROJI BIN SARMIDI Advisor, 2nd ICA Research Symposium
2nd ICA RESEARCH SYMPOSIUM 2019 |5 MESSAGE FROM The Chairman of 2nd ICA Research Symposium, Dr. Cheng Kian Kai Ladies and Gentlemen, It is my great pleasure to welcome you to the 2nd ICA Research Symposium 2019 here in Pagoh. This year, the theme of the symposium is “Agriculture and Food Innovation” focusing on recent advancement in agriculture technology and food science research. This symposium is an event organized by Universiti Teknologi Malaysia, in collaboration with the Malaysian Ministry of Education, Universiti Tun Hussein Onn Malaysia, International Islamic University Malaysia and Politeknik Tun Syed Nasir at Pagoh Higher Education Hub. Together, the symposium will provide a platform for the exchange of authoritative views by leading researchers as well as industrial leaders in this exciting field. It will also provide a great opportunity to all participants to share latest research findings and innovative ideas in agriculture and food science with professionals from academia and industry. I am certain we will find the symposium rewarding and enjoyable with opportunity for friendship and collaboration. I wish to express my heartfelt appreciation to members of Organizing Committee for their hardwork and preseverance, distinguish keynote speakers, paper presenters, participants and also to sponsors and exhibitors for their contribution in making 2nd ICA Research Symposium 2019 a success. Finally, I wish all participants to have a delightful and stimulating symposium. Thank you. DR. CHENG KIAN KAI Chairman of the 2nd ICA Research Symposium 2019
2nd ICA RESEARCH SYMPOSIUM 2019 |6 PREFACE The 2nd ICA Research Symposium (ICARS) 2019 is organized by Universiti Teknologi Malaysia, in collaboration with the Malaysian Ministry of Education, Universiti Tun Hussein Onn Malaysia, International Islamic University Malaysia and Politeknik Tun Syed Nasir. The 2nd ICA Research Symposium 2019 will provide a platform for the exchange of authoritative views by leading researchers as well as industrial leaders in agriculture technology and food science research. The 2nd ICARS 2019 will feature a comprehensive scientific programme highlighting the biotic, natural & organic farming, beneficial microorganism, bioactive compounds for health & wellness, nutrient dense & functional food, and socioeconomics in agriculture & food production. It will also include areas of current interest such as sustainable environment, Internet of Things (IoT) & precision farming. The scientific programme will culminate with leading industry players that will see practicality for the latest development of agriculture technology and food science research. Since its inception in 2018, the ICARS annually attracts researchers, scientists, young professionals, and industry player from agriculture and food sector to network and exchange scientific and case studies on a wide range of agriculture technology and food science issue. The organizing committee sincerely hopes that this symposium provides all involved with interesting and multidimensional perspectives and relevant information which is useful for your professional and personal use. Finally, we hope that this 2nd ICARS 2019 opens up new opportunities for delegates to contribute and exchange ideas with like- minded peers in the field of agriculture technology and food science.
2nd ICA RESEARCH SYMPOSIUM 2019 |7 TENTATIVE PROGRAMME TIME PROGRAMME 0730 – 0800 Registration Welcoming speech by Dr. Cheng Kian Kai, Director, Innovation Centre in Agritechnology for Advanced Bioprocessing, Universiti Teknologi Malaysia 0800 – 0830 Opening speech by Prof. Dr. Rosli bin Md. Illias, Dean, Research Alliance of Health and Wellness, Universiti Teknologi Malaysia Opening Ceremony Keynote – “Soil Health and Nutrient Dense Crops” by Prof. Dr. Mohamad 0830 – 0850 Roji Sarmidi, Innovation Centre in Agritechnology for Advanced Bioprocessing, Universiti Teknologi Malaysia Industry – “Smart Farming: Revolutionizing The Malaysian Agriculture 0850 – 0910 Industry” by Mr. Lau Bik Soon, Group Chief Executive Officer, REDtone International Berhad 0910 – 0940 Group photo, morning tea break & poster session Session 1 (Chaired by Dr. Cheng Kian Kai): 0940 – 0950 • Nutrient dense & functional food • Bioactives compound for health & wellness Session 2 (Chaired by Dr. Nor Zalina Othman): 1100 – 1220 • Sustainable environment • Agriculture and food technology 1220 – 1400 Lunch & Poster Session Industry – “EM and Microbiome” by Mr. Yoshitaka Fukugauchi, Country 1400 – 1420 Manager, EMRO Malaysia Sdn. Bhd. Industry - “Embracing sustainable agriculture with EM technology” by 1420 – 1440 Mr. Jeff Tai, Director, Zenxin Agriculture Sdn. Bhd.
2nd ICA RESEARCH SYMPOSIUM 2019 |8 Session 3 (Chaired by Dr. Umi Aisah Asli): 1440 – 1550 • Internet of Things (IoT) & precision farming • Sustainable environment • Other topics 1550 – 1610 Break & Poster Session Session 4 (Chaired by Dr. Ong Pei Ying): 1600 – 1700 • Sustainable environment • Other topics 1700 – 1715 Closing Ceremony 1715 until Buffet Dinner end
2nd ICA RESEARCH SYMPOSIUM 2019 |9 ORAL PRESENTATIONS SESSION 1 Nutrient dense & functional food Bioactives compound for health & wellness Amino acid profiling for Clean and Unclean 1 Nurul Alia Azmi 0950 - 1000 Edible Bird’s Nest Effect of Aqueous Extraction Temperature Dr. Noor Soffalina 2 1000 - 1010 on Physicochemical Properties of Okra Sofian Seng Gum (Abelmoschus esculentus) Momordica charantia suppresses 3 Lee Shi Yan 1010 - 1020 inflammation and glycolysis in LPS- activated RAW264.7 cells Use of antioxidant active gelatin films to Dr. Noorul Syuhada enhance the oxidative stability of cooked 4 1020 - 1030 Mohd Razali chicken breast meat stored in aerobic and vacuum-skin packaging conditions Enhancing The Total Phenolic Content and Rachael Kaur A/P Jagjit 5 1030 - 1040 Antioxidant in Dates Fruit By Applying Singh UVC Radiation Antioxidant properties of Hylocereus Siti Nor Azlina Abd 6 1040 - 1050 polyrhizus aqueous extract and its effect to Rashid lipid stability in bakery product on muffin Characterization of pyroligneous acid Raja Safazliana Raja 7 1050 - 1100 produced from microwave-assisted Sulong treatment of palm kernel shell SESSION 2 Biotic, natural and organic farming Beneficial microorganism Dr. Mohd Fitri Mohd Designing of organic fertilizer from food 8 1110 - 1120 Yakub waste Volatile organic compounds produced by Dr. Raja Kamarulzaman 9 1120 - 1130 fermentation process of stingless bee Raja Ibrahim honey Aiman Farhana Entrapment of Volvariella Volvacea Spores 10 1130 - 1140 Azaddin in Electrospun Nanofibers Mats A short review on recent development of Norsyafiqah Amalina 11 1140 - 1150 laccase immobilization on different Ahmad Jafri support materials Potential of Ficus carica leaf extract in 12 Norashikin Anjur 1150 - 1200 bacterial disease treatment of Tilapia (Oreochromis niloticus) in vivo Effect of Organic Pesticide in Controlling 13 Dr. Norahim Ibrahim 1200 - 1210 Pest and Disease in Capsicum Cultivation Phenotypic and Genetic Variation of 14 Zulaikha Sarobo 1210 - 1220 Capsicum annuum Germplasm Collection
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 10 SESSION 3 Internet of Things (IoT) & precision farming Sustainable environment Other topics Nur Alyani Nadhiya Control and monitoring system for 15 1450 - 1500 Fakharulrazi livestock feeding time via smartphone Dr. Mohd Fitri Mohd Building a smart gardening system and 16 1500 - 1510 Yakub plant monitoring using IoT Ainur Nalisa Abd Quality Assessment of Meats Using FT-IR 17 Rashid / Dr. Saliza 1510 - 1520 Spectroscopy, Texture Analyzer, Color Asman Spectrophotometer and Image Analysis Nuraznee Mashodi / Detection of Extra Virgin Olive Oil 18 1520 - 1530 Saliza binti Asman Adulteration Using FTIR Spectroscopy Anis Shahira Nor Dye-Adsorption Characteristics of Biochar 19 1530 - 1540 Effendi Produced From Coconut Husk Intelligent Home 1.0 System Based on 20 Mustafa Kamal Surif 1540 - 1550 Arduino Platform SESSION 4 Sustainable environment Other topics Rapid Discrimination of Porcine and Tilapia Gelatin using Fourier Transform Dr. Norhidayu 21 1610 - 1620 Infrared- Attenuated Total Reflection Muhamad Zain (FTIR-ATR) combined with 2 Dimensional Infrared (2DIR) Correlation Analysis Production of Pre-Treatment Agent of 22 Thanes Vengades 1620 - 1630 Used Cooking Oil Using Paddy Husk Dr. Syaripah Za'imah Bioproduct From Chicken Waste Using 23 1630 - 1640 Haji Syed Jaapar Ultrasonic Extraction Method Utilization of Used Cooking Oil and Methivanan A/L 24 1640 - 1650 Cassava Peel as Degradable Bioplastic Loganathan Film Production Of Corrosion Inhibitor By 25 Azmir Iqbal Ibrahim 1650 - 1700 Using Piperine From Black Pepper (Piper Nigrium)
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 11 POSTER PRESENTATIONS Session: 1200 - 1400 Acute toxicity study of local Ziziphus mauritiana (bidara) 1 Dr. Arnida Hani Teh fruit extract Characterisation of Asystasia gangetica and Phyllanthus Dr. Ozahrah Hasan 2 niruri Extracts: Total Phenolic Content, Antioxidant and Basir Antibacterial Activities Dr. Nadiatur Akmar 3 A1 and A2 milk: A Review Zulkifli Plant growth performance of maize (Zea mays L.) cultivars 4 Mohd Farid Ismail influenced by different fertilizer application rate and method under biotic farming condition Effect of organic and inorganic fertilization on soil organic Nur Amalina Mohd 5 matter, carbon and nitrogen accumulation in a newly Ropi cultivated farmland Session: 0850 - 0920 Carbon and Nitrogen Accumulation in Abelmoschus Norfakhrina Mohd esculentus, and Vigna unguiculata subsp. sesquipedalis 6 Noor treated with different fertilizer regime Under Polyculture System Monoculture vs Polyculture System: Effect of culture 7 Noorafizah Dzahir system on Okra, Yardlong Bean, Water Spinach Root Growth Germination and Vegetative Performance of F1 and F2 8 Salimah Ab Malik Generations of Okra Zaheda Mohamad Nutritional Requirements for Growth of Azotobacter 19 Azam chroococcum with Potential as Biofertilizer The response of extracellular soil enzyme activities under 10 Suhir Sulaiman different treatment of fertilizer in polycropping system Energy conversion from human heat into electricity using 11 Mohd Azlan Jalal thermoelectric generator The effects of Weeping Willow (Salix babylonica) grounds Mohd Nadzreen 12 in soil mix as growing media for Choy Sum (Brassica Hidayat Sarjuni chinensis Var. Parachinensis) Interaction of rutin with selected polyphenols affects its 13 Khairunnisa Embi total antioxidant activity Potential of Lemongrass (Cymbopogon citratus) Extract as 14 Norashikin Anjur Antiparasite in Tilapia (Oreochromis niloticus) Culture In Vitro Antioxidant and Antimicrobial Activities of 15 Nur Fashya Musa Garcinia cambogia 16 Rozaliana Ab Karim Vertical Cultivation System for Sustainable Farming A Rafidah A Mohd Development and Sensory Attributes of Mixed Bitter Gourd 17 Yunos (Momordica charantia) and Green Apple Juice Puvendaran A/L 18 Synthesis of Natural Coagulant from Petai Belalang Peel Neelamalai Relationship between the Exchangeable bases and the Soil 19 Hajar Aminah A. Karim Cation Exchange Capacity: Case Studies of Oil Palm Plantation in Malacca
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 12 The Simultaneously Deinking and Bioethanol production of 20 Mohamad Azzuan Rosli Recycle Office Paper to Enhance Environmental Sustainability The Preparation of Pour Point Depressant of Waxy Crude 21 Kausalyaa Oil Optimization of cell mass production and functional 22 Nur Sazwani Daud characteristic of exopolysaccharide from Paenibacillus polymyxa The Effect of Solid-To-Liquid Ratio and Particle Size on the 23 Norliza Abdul Latiff Extraction of Quercitrin from Cosmos Caudatus (C.C) Development of an integrative process for the production Dr. Norfahana Abd 24 of microbial biomass protein (MBP) from rice straw for Talib animal feeds application A development of a model on Human Resource Zaheed Husein 25 Management for the sustainability of the Agriculture sector Mohammad Al Din in Bangladesh
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2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 15 SCIENTIFIC COMMITTEE MEMBERS Mohamad Roji Sarmidi, Prof. Dr. (UTM) Cheng Kian Kai, Dr. (UTM) Umi Aisah Asli, Dr. (UTM) Nor Zalina Othman, Dr. (UTM) Nicole Leong Hong Yeng, Dr. (UTM) Muhammad Helmi Nadri, Dr. (UTM) Ong Pei Ying, Dr. (UTM) Lee Chew Tin, Prof. Dr. (UTM) Harisun Yaakob, Dr. (UTM) Rosnani Hasham @ Hisam, Dr. (UTM) Saleha Shahar, Dr. (UTM) Yanti Maslina Mohd Jusoh, Dr. (UTM) Shakila Abdullah, Dr. (UTM) Mohd Hefni Rusli, Dr. (MPOB) Wan Abd Al-Qadr Imad Wan Mohtar, Dr. (UM) Saiful Irwan Zubairi, Dr. (UKM) Aida Firdaus Muhammad Nurul Azmi, Dr. (UiTM) Wong Won Fen, Dr. (UM) Mohd Fadzelly Abu Bakar, Prof. Madya Ts. Dr. (UTHM) Razauden Mohamed Zulkifli, Dr. (UTM) Samshul Amry Abdul Latif, Dr. (UIAM) Norahim Ibrahim, Dr. (UTM) Wan Mohd Nuzul Hakimi Wan Salleh, Dr. (UPSI) Zainul Akmar Zakaria, Prof. Madya Dr. (UTM) Asraf Fizree Mohamad@Abdullah, (Politeknik Jeli, KPM) Lee Ting Hun, Dr. (UTM) Sity Aishah Mansur, Dr. (UTHM) Nadirul Hasraf Mat Nayan, Dr. (UTHM)
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 16 ORGANIZING COMMITTEE MEMBERS ADVISOR Prof. Dr. Mohamad Roji Sarmidi CHAIRMAN Dr. Cheng Kian Kai VICE CHAIRMAN Dr. Nicole Leong Hong Yeng SECRETARY Publicity Mohd Farid Rahmat Mohd Farid Ismail Rozaliana Ab Karim Mohammad Azhari Abdul Rahman Nor Azilah Abd Aziz Khairul Nizam Hamzah Noraimy Kamisan Marketing TREASURER Dr. Aidee Kamal Khamis Aini Fariza Ahmad Muhammad Rahmat Naim Ab Rahman Rafidah Abdul Rahim Noor Azizah Hj. Mansor Logistic Abd Salihul Halim Wagiman Khairilanuar Mohd Hanim Mohd Azlan Jalal AUDITOR (post event) Nurul Aishah Husin Safety Shahwalludin Ghazali SCIENTIFIC COMMITTEES Hairulnizam Gendot Dr. Muhammad Helmi Nadri A.Rafidah A. Mohd Yunos Hospitality Muhammad Hazim Yusof Siti Nor Azlina Abd Rashid Salimah Ab Malik COMMITTEES Khairunnisa Embi Registration Sponsorship Siti Norsalihin Md Radzi Dr. Umi Aisah Asli Hajar Aminah A. Karim Noorafizah Dzahir Pre Symposium Workshop Nurliza Abd Rahman Dr. Nor Zalina Othman Rafizah Rauf Zaheda Mohamad Azam Siti Saadiyah Ismail Mohamad Azzuan Rosli Nardhiha Nadia Mastan Post Symposium Open Day Dr. Ong Pei Ying Mohd Nadzreen Hidayat Sarjuni Zulaikha Sarobo
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2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 18 BIOGRAPHY OF KEYNOTE AND INVITED SPEAKERS
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 19 Keynote Speaker 1 Prof. Dr. Mohamad Roji Sarmidi Innovation Centre in Agritechnology for Advanced Biprocessing Biography Prof. Dr. Mohamad Roji bin Sarmidi is a Professor of Bioprocess Engineering at Universiti Teknologi Malaysia (UTM). His research interests is in the development of novel bioproducts for human health and wellness. He is the Chairman of the board of directors of Phyto Biznet Sdn Bhd, a spin off company providing wellness solutions for healthy living. He is also the Deputy President of the Association of Nutritional Medicine Practitioners Malaysia (MNMedP). He was the recipient of the 11th National Academy Award under the Product Innovation and Commercialization category in 2017.
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 20 Invited Speaker 1 Mr. Jeff Tai Director of Zenxin Agriculture Sdn Bhd Biography Mr. Jeff Tai, born on April 1981, is the eldest son of Mr. Tai Kok Kong. He was doing research since year 2004 in Nanyang Technological University for 3 years and achieved his Master of Sciences (Life Sciences). Before this, he graduates as Bachelor of Scienced (Biochemistry) from National University of Singapore in 2003. He completed his secondary education in Kluang Chong Hwa Chinese School in year 1999. He joined Zenxin Agri-Organic Food Sdn Bhd at year 2007, whi involved in the organic fruits and vegetable crops cultivation. At the same time, he manages organic fertilizer division of Zenxin Agriculture Sdn Bhd and is responsible for technical and operation of the fertilizer factory. With his research background and years of experience and knowledge in the agriculture industry, he effectively leads the fertilizer division to another pinnacle. In year 2012, he became the Executive Director of Zenxin Plantation Sdn Bhd, who leads the management of the oil palm estates operation nationwide for the land bank of 4,200 acres. In year 2015, he became the Director of Zenxin Agriculture Sdn Bhd, who take charge of sales and marketing department of the layer farm with daily production of 600,000 eggs daily.
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 21 Invited Speaker 2 Mr. Yoshitaka Fukugauchi Country Manager, EMRO Malaysia SdnBhd Biography Mr. Yoshitaka Fukugauchi has been working in EM Research Organization in Okinawa, Japan, since 1996. He was active in the African continent and was a manager in South Asia, before moving to Malaysia in 2012 to promote EM Technology in Malaysia, Singapore, Indonesia, Philippines, Australia and New Zealand. Mr. Fukugauchi is currently the Managing Director of EMRO Malaysia in Johor.
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 22 Invited Speaker 3 Mr. Lau Bik Soon Group Chief Executive Officer, REDtone International Bhd. Biography Mr. Lau Bik Soon was appointed to the Board of Directors of the Company on 13 August 2008. He assumed the position of REDtone’s Group Chief Executive Officer on 8 July 2011. Mr. Lau has a First Class Honours Degree in Electrical Engineering from University Technology Malaysia. Having guided the Company to achieve a firm footing in the data and broadband space, Mr. Lau will continue to play a significant role in driving REDtone as it expands its spectrum of services. He was awarded the 2014 Asia Pacific Entrepreneurship Awards, a regional award for outstanding entrepreneurship. His extensive experience in the ICT and telecommunications industry spans over 24 years during which he held key positions with international organizations such as Cisco Systems, Sun Microsystems, Compaq Computer, TQC Consultant (IT Division) Sdn Bhd and Motorola. He won numerous sales management excellence awards and accolades during his time there. Prior to joining REDtone, he was the Country Manager for Hitachi Data Systems Malaysia.
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2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 25 ABSTRACTS
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 26 Keynote Soil Health and Nutrients Dense Crops Prof. Dr. Mohamad Roji Sarmidi Innovation Centre in Agritechnology for Advanced Biprocessing, University Teknologi Malaysia Malaysia allocates about 7.8 million hectares of agriculture land for the planting of cash, food, plantation and horticulture crops providing sustainable economic growth and food security. Thus it is important to maintain and enhance the quality of the agriculture land to ensure a sustainable future. The presentation is to cover the role of healthy soil in the production of sustainable high yield nutrient dense crops for the support of healthy population. The role of nutrient dense crop for the support of a healthy cellular metabolism will be highlighted. Practical method to promote soil health and soil remineralization will presented. Healthy soil with the appropriate physical-chemical-biological parameters provide the plants with optimum access to water, air and nutrients for optimum growth. Healthy nutrient dense crops are crucial for the health grazing animals and human population as nutrient dense crops exhibit a higher content of micronutrients, minerals and phytochemicals. In addition, nutrients dense crops are superior in their taste, texture and aroma and are known to a have a longer shelf life. Their resistant to plant diseases and pest are also higher so are their productivity. By having a healthy relationship with agriculture land, our health and wellbeing are rest assured.
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 27 ORAL PRESENTATIONS
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 28 Oral presentation 1 Amino acid profiling for cleaned and uncleaned edible bird’s nest N.A. Azmi1, T.H. Lee1*, & C.H. Lee1 1Schoolof Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor Abstract Edible Bird’s Nest (EBN) is a secretion produced by swiftlets. It is traditionally used as natural health supplement and functional food. It is one of the most expensive animal by-products. The quality of EBN in the current market is solely based on the physical appearance but not based on in its composition. Hence, the purpose of this study was to develop a standardized sample preparation method and to analyze the amino acid profile for the cleaned and uncleaned EBN. A total of 65 samples were randomly collected from 13 states in Malaysia (5 pieces each states). Amino acid composition of EBN samples were determined by High Performance Liquid Chromatography (HPLC). Data obtained was analysed by Independent t-test analysis, Person Correlation analysis, Principal Component analysis and Orthogonal Partial Least Square Discriminant analysis (OPLS-DA). There was significant difference between cleaned and uncleaned EBN in all amino acids detected except glycine, tryptophan, alanine and proline. There were highly significant different correlations among amino acids in cleaned and uncleaned EBN samples. The model constructed by OPLS-DA was found to be a promising tool with high predictive power of 76.1%. Robustness of the model was validated and blind test samples were correctly assigned to their respective cluster. In addition, this analysis was able to select statistically significant components according to Variable Importance on Projection (VIP). Our methodology clarified significant difference between cleaned and uncleaned EBN and providing new insights to the EBN study. Keywords: edible bird’s nest, quality indicator, cleaned and uncleaned samples, amino acid * Corresponding author Name: Dr. Lee Ting Hun Address: School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor Email: leetinghun@utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 29 Oral presentation 2 Effect of aqueous extraction temperature on physicochemical properties of okra gum (Abelmoschus esculentus) N.-S. Sofian-Seng1*, & Z. H. Rashid Ridha1 1Centre for Biotechnology and Functional Food, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor. Abstract Okra gum is the polysaccharides-rich mucilage obtained from okra (Abelmoschus esculentus). This work aims to determine the physicochemical properties of extracted okra gum at different aqeuous extraction temperature (25, 40, 60, and 80℃). Physicochemical analyses include determination of water holding capacity, viscosity, Total Phenolic Content (TPC) as well as antioxidant activities (2,2-diferil-1-picrihydrazyl Radical Scavenging Activity (DPPH) and Ferric Reducing Antioxidant Power (FRAP) analyses). Results showed that the yield of extracted okra gum has no significant difference (p>0.05) at extraction temperature of 25℃ and 40℃. However, 60℃ and 80℃ showed significant increase (p
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 30 Oral presentation 3 Momordica charantia suppresses inflammation and glycolysis in LPS-activated RAW264.7 cells Shi Yan Lee1, Won Fen Wong2, & Kian-Kai Cheng1* 1 Innovation Centre in Agritechnology, Universiti Teknologi Malaysia, 84600 Pagoh, Johor, Malaysia 2Departmentof Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur. Abstract Momordica charantia, also known as bitter gourd, is a climber that is widely cultivated and available in Asia, India, East Africa, and South America. Although M. charantia is known for its anti-diabetic and anti-inflammatory activities, the mechanism of its anti- inflammatory action is yet to be fully understood. The current study aimed to examine the bioactivity of M. charantia in modulating LPS-induced inflammation in murine RAW 264.7 macrophages. Briefly, real-time PCR was applied to analyze the expression of genes involved in inflammatory pathway and glucose metabolism in the lipopolysaccharide (LPS) activated RAW264.7 cells treated with or without M. charantia. The current results showed that the mRNA level of pro-inflammatory cytokines such as IL6, TNF-α, IL1B, glycolytic gene such as HK2 and the main glucose transporter GLUT1 were upregulated significantly by LPS. Notably, these changes in gene expression can be inhibited by M. charantia treatment. Furthermore, M. charantia was found to inhibit LPS-induced on NF-κB (p65) nuclear translocation, which can be linked to downregulation of IκB-α phosphorylation. Previously, upregulated glycolysis, also known as the Warburg effect, has been associated with inflammation event in RAW264.7 cells. Consistently, our results showed that induction with LPS increased the consumption of glucose and lactate production in activated RAW264.7 cells, but the upregulated glycolysis can be inhibited by M. charantia treatment. Taken together, the current study provided evidence suggesting an association between anti-inflammatory activity of M. charantia with downregulation of glucose metabolism. The current findings contribute to the accumulating evidences showing the potential of M. charantia in providing therapeutic effect to inflammation and inflammation-related disorders. Keywords: Momordica charantia, anti-inflammation, glucose metabolism, gene expression, macrophage * Corresponding author Name: Dr. Cheng Kian Kai Address: Innovation Centre in Agritechnology, Universiti Teknologi Malaysia, 84600 Pagoh, Johor, Malaysia Tel: 06-9742801 Email: chengkiankai@utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 31 Oral presentation 4 Use of Antioxidant Active Gelatin Films to Enhance the Oxidative Stability of Cooked Chicken Breast Meat Stored in Aerobic and Vacuum-skin Packaging Conditions. M. R. Noorul Syuhada1*, M. O’Grady2, & J. P. Kerry2 1Centre for Biotechnology and Functional Food, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia 2Food Packaging Group, School of Food and Nutritional Sciences, College of Science, Engineering and Food Science, University College Cork, Ireland Abstract Two mixtures of grape seed, olive leaf and sweet chestnut bark (GS/OL/CB1 and GS/OL/CB2) and tea catechin (TC) extract (positive control) were incorporated into active gelatin films at two concentrations (0.7 and 1.5 mg/ml). The effect of active films on the oxidative stability of cooked chicken breast meat was examined. Film solutions were casted and dried (25°C, 50% RH) for 48 hr. The thickness, colour, light transmission, opacity, water vapour permeability (WVP) and oxygen permeability (OP) of control and active films were investigated. Cooked-sliced-cored chicken breast samples were overwrapped with control and active films and stored in aerobic and vacuum-skin packaging at 4°C for up to 6 and 18 days, respectively. The ability of active films in influencing the lipid oxidation of cooked chicken was investigated. Both concentrations of the active films had similar thickness, contact angle and WVP relative to the controls. High concentration TC films had lower (p
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 32 Oral presentation 5 Enhancing The Total Phenolic Content and Antioxidant in Dates Fruit by Treatment with UVC Radiation Rachael Kaur Jagjit Singh1*, Ainnur Balqis Zulikiflee1, Nor Hairul Palal1, Rahimawati Abdul Rahim1 1Politeknik Tun Syed Nasir Syed Ismail, Hab Pendidikan Tinggi Pagoh, KM 1 Jalan Panchor 84600 Pagoh, Johor Abstract Dates are important cause there are believe to be rich in nutrients and has many beneficial nutrients particularly polyphenols which have antioxidant properties. Previously, UV-C radiation was found to promote antioxidant and bioactive compounds of fresh fruit and vegetables. The aim of this study was to enhance the total phenolic content and antioxidant activity by applying UV radiation in Medjool dates fruit, leading more bioactive compounds available for extraction. In this study, small cube of dates were placed under a UV lamp for different duration while untreated dates was used as a control. The total phenolic content and antioxidant were significantly affected by UV-C radiation. A longer treatment time led to the higher amount of total phenolic content and antioxidant activity. Notably, the UV radiation exposure are more effective at 180 seconds than other time limit to increase the antioxidant and total phenolic content in Medjool date fruits. The results of this study showed that UV-C treatment has the potential to increase the extraction of bioactive compounds of dates fruits. Dates fruit is considered a source of antioxidant. Keywords: Medjool dates, UV-C radiation, Antioxidant, Total phenolic * Corresponding author Name: Rachael Kaur A/P Jagjit Singh Address: No 3 Jalan St3 Lot 3521 Kampung Baru Kundang 48050 Rawang Selangor Tel: 018-4004236 Email: rachaelkaur99@gmail.com
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 33 Oral presentation 6 Antioxidant properties of Hylocereus polyrhizus aqueous extract and its effect to lipid stability in bakery product on muffin Siti Nor Azlina Abd Rashid1*, Noriham Abdullah2, Cheng Kian Kai1, Leong Hong Yeng1 1 Innovation Centre in Agritechnology, Universiti Teknologi Malaysia, 84600 Pagoh, Johor, Malaysia 2 Faculty of Applied Science, Universiti Teknologi Mara (UiTM), 40450, Shah Alam, Selangor, Malaysia Abstract Baked goods contained high amount of fat and are prone to oxidative rancidity that can decrease its shelf life to less than a week. Synthetic preservatives have commonly been used in commercial bakery products. The use of synthetic preservatives has long been a concerned for its safety and long-term health effects. Due to this, safe and natural preservatives is highly demanded. The bright red colour of red pitaya fruit (Hylocereus polyrhizus) has gained increasing interest for its antioxidative potential to be used as natural preservatives. In this study, aqueous extract of skin and flesh of pitaya fruit were analysed for its antioxidant activity and its effect on the oxidative stability of fat in muffin was investigated. Firstly, the antioxidant activity of the red pitaya flesh and peel aqueous extracts were determined. The total phenolic contents (TPC) and the antioxidant activity were analyzed using 1,1-diphenyl-2-picrylhydrazyl free radical scavenging (DPPH) assay and its IC50 were compared to synthetic antioxidant; butylated hydroxyanisole (BHA). Then, the extract with the highest properties of antioxidant was added into muffin and its effect on the quality of muffin were determined by using peroxide value (PV) assay. The TPC of the flesh and peel were 0.21 mg g-1 and 0.25mg g-1 gallic acid equivalent respectively. The IC50 DPPH scavenging power of peel and flesh extract is 2900 ppm and 3270 ppm respectively. The peel aqueous extract was then chosen to be added into muffins and the PV analysis showed that the extract was able to delay the oxidative rancidity of fat in the muffin and extend its shelf life for more than 8 days and were comparable to BHT. The study indicated that the aqueous peel extract from pitaya has strong antioxidant properties and can be used as a potential natural antioxidant in food to prolong the shelf life. Keywords: Hylocereus polyrhizus, antioxidants, lipids, muffin, peroxide value * Corresponding author Name : Siti Nor Azlina Bt Abd Rashid Address: Innovation Centre in Agritechnology, Universiti Teknologi Malaysia, 84600 Pagoh, Johor, Malaysia Tel. no.: 06-9742859 Email: sitinorazlina@utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 34 Oral presentation 7 Characterization of pyroligneous acid produced from microwave-assisted treatment of palm kernel shell R.S. Raja Sulong2, S.E. Zulkifli2, R. Hasham2, & Z.A. Zakaria1* 1Schoolof Chemical & Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Johor Bahru, Johor, Malaysia 2Instituteof Bioproduct Development, Universiti Teknologi Malaysia, 81310, Johor Bahru, Johor, Malaysia Abstract Oil palm plantation is one of the largest cultivation in Malaysia. The massive production of palm oil has led to huge palm oil biomass by product such as palm kernel shell that has become an increasingly threatening environmental issue. Conversion of biomass through microwave pyrolysis has become one of the solutions to manage the abundance of biomass. Therefore, the aim of this study was to utilize the palm kernel shell for the production of pyroligneous acid (PA) by using microwave-assisted pyrolysis which would then be evaluated for its total phenolic content (TPC) and scavenging DPPH free radicals. Pyroligneous acid of palm kernel shell will be produced from the condensation of smoke generated during pyrolysis process. From this study, the TPC observed was 49.96 mg GAE/g, whereas for the IC50, DPPH value obtained was, 66.19 µg/mL. Pyroligneous acid produced during the pyrolysis process has the potential to be applied in various applications and could serve as an alternative eco-friendly source of natural antioxidant. Keywords: Biomass, Palm Kernel Shell, microwave-assisted pyrolysis, pyroligneous acid * Corresponding author Name: PM Dr Zainul Akmar bin Zakaria Address: School of Chemical & Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Johor Bahru, Johor, Malaysia Tel: 012-6270423 Email: zainul@ibd.utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 35 Oral presentation 8 Designing of organic fertilizer from food waste through implementation of internet of things M.N. Baba1, L.F. Zhao1, A. Rafiq1, H.H. Yudu1, N.A. Fakharulrazi2, F. Yakub2*, A. Azizan3 1Department of Electronic System Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia 2Wind Engineering for Environment Laboratory, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia 3Razak Faculty of Technology and Informatics, Universiti Teknologi Malaysia, 54100 Kuala Lumpur, Malaysia Abstract The objective of this paper is to build a fully automated composting machine that can help to reduce food waste using a more efficient and environmental friendly method. This machine has its special features of heating, cooling and grinding which is simple and easy to use for every consumer at just one touch of a button. In addition, it uses a special filter to eliminate unpleasant odor to ensure consumer’s peace of mind. The process of composting uses node microcontroller (MCU) to run its operation and using Internet of Things (IoT) with a developed mobile application to measure the amount of food waste, current process and its moisture content before turning the waste into high nutrient flakes at around 10% of its original volume. It will also notify the consumer when the whole process is done and the final product is ready to use. The produced flakes are good for nurturing soils, use as fertilizer, and renewable source of energy or animal feed. The benefit is to help reduce handling cost at landfill. Excessive logistical energy is required to send food waste to landfill if applied conventional equipment. This product has a high potential to penetrate the end users who usually cooks at home and also the industrial food manufacturer, whether from medium to large which produces a lot of raw waste. Essentially, this machine can result less food waste been thrown through implementation of IoT and change it to usable fertilizer also helps in pest control. Keywords: food waste, automated composting machine, MCU microcontroller, Internet of Things * Corresponding author Name: Fitri Yakub Address: Wind Engineering for Environment Laboratory, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia Tel. no.: 03-22031286 Email: mfitri.kl@utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 36 Oral presentation 9 Volatile organic compounds produced by fermentation process of stingless bee honey R.K. Raja Ibrahim1,2*, K.N.A. Mohammed Hassan1, & H.H. J. Sapingi1,2 1,2 Department of Physics, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia 2Laser Centre T05, Ibnu Sina ISI/SIR, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia Abstract This work focuses on analyzing stingless bee honey quality based on volatile organic compounds (VOCs) produced by natural fermentation process of honey. Fresh stingless bee honey harvested at the same bee farm was divided into eight samples with each contains 100 ml and filled into a tight container. The VOCs produced was then collected and filled into a gas chamber with 8 m effective optical pathlength. Fourier transformed infrared spectrometer (FTIR) was used to detect and subsequently quantify the VOCs collected from each honey sample over a month period. FTIR results revealed that ethanol, acetic acid, and methanol are among VOCs produced by the process. FTIR analysis of VOCs profile suggested that it has potential to be used in determining stingless bee honey quality and purity. Keywords: VOCs, stingless bee honey, FTIR, ethanol * Corresponding author Name: Raja Kamarulzaman Raja Ibrahim Address: Department of Physics, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia Tel: 019-7544121 Email: rkamarulzaman@utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 37 Oral presentation 10 Entrapment of Volvariella Volvacea Spores in Electrospun Nanofibers Mats Aiman Farhana Azaddin1, Roshanida A. Rahman2*, Rosli Md Illias3, Mazura Jusoh4, Juhana Jaafar5 1Department of Bioprocess, School of Chemical Engineering & Energy Engineering, UTM 2Department of Bioprocess, School of Chemical Engineering & Energy Engineering, UTM 3Department of Bioprocess, School of Chemical Engineering & Energy Engineering, UTM 4Department of Chemical Engineering, School of Chemical Engineering & Energy Engineering, UTM 5Advanced Membrane Technology Research Centre (Amtec), School of Chemical Engineering & Energy Engineering Abstract Nanofibers mats containing Volariella volvacea spores was prepared by electrospinning technique. It has been one of the promising and alternative technique in immobilizing microorganism cells including bacteria, virus and fungi through the entrapment process. The advantages that offered by the nanosize of the electrospun nanofibers exhibit outstanding properties includes high porosity and reusability which make them an ideal candidates for various type of potential application especially in bioprocess development. In the present study, the spores of Volvariella volvacea was suspended in a series of polymer solutions (poly (vinyl alcohol) and polyvinyl pyrrolidone) and subjected to an electrostatic field supplied by the power voltage. The viability of the entrapped spores was estimated through microorganism cells test by culture the obtained nanofibers mats on potato dextrose agar (PDA) and studying their growth rate. Meanwhile, the presence of the spores was determined by using scanning electron microscopy (SEM). The present result demonstrated that electrospinning process possess high potential in entrapping and immobilization of living microorganism cells. Keywords: Entrapment, Electrospinning, Volvariella volvacea, viability *Corresponding author Name: PM. Dr. Roshanida binti A. Rahman Address: Department of Bioprocess, School of Chemical Engineering & Energy Engineering, Faculty of Engineering Tel: 013-7520497 Email: roshanida@cheme.utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 38 Oral presentation 11 A short review on recent development of laccase immobilization on different support materials A.J. Norsyafiqah Amalina, A. Noorhalieza, & A.R. Roshanida* School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor Abstract Laccase is a bio catalytic agents and multi-copper enzyme containing oxidases that has great potential in oxidizing large number of phenolic and non-phenolic compounds. However, low stability, high production cost, non-reusability, sensitive towards denaturing and poor storage ability of free enzymes are the limitations occurred for large scale use of laccase. These problems lead to the progress in laccase immobilization in order to facilitate the efficient recovery and re-use of the enzyme, thus enabling cost- effective in continuous processes. Apart from discussing on different methods in laccase immobilization such as entrapment, encapsulation and cross-linking in general, we review a recent development in laccase immobilization on different supports or carriers binding (natural and synthetic). Future works should focus on the innovative strategies on the modified supports to improve the enzyme immobilization as well as a sensible entrapment technique for the industrial application. Keywords: Laccase, immobilization, support materials * Corresponding author Name: PM Dr. Roshanida A. Rahman Address: School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor. Tel: 07-5558436 Email: r-anida@utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 39 Oral presentation 12 Potential of Ficus carica leaf extract in bacterial disease treatment of Tilapia (Oreochromis niloticus) in vivo A. Norashikin1*, S.F. Sabran1,2, & S.M. Syukri3 1Department of Technology and Natural Resources, Faculty of Applied Sciences and Technology (FAST), Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Educational Hub, 84600 Pagoh, Muar, Johor, Malaysia 2Centre of Research for Sustainable Uses of Natural Resources (CoR-SUNR), Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Educational Hub, 84600 Pagoh, Muar, Johor, Malaysia 3Department of Agrotechnology and Bio-industry, Politeknik Sandakan, Education Hub, Jalan Sg. Batang, Batu 10, 90000 Sandakan, Sabah, Malaysia Abstract Fifteen days of study was conducted to observe the potential of Ficus carica leaf extract in the treatment of tilapia (Oreochromis niloticus) infected by Aeromonas hydrophila. A. hydrophila commonly found as pathogenic bacteria in aquaculture. This study is projected to the application of plant extracts in order to replace antibiotics used towards green aquaculture. 120 fish were injected intraperitoneally with 0.1ml of A. hydrophila (106 cfu ml-1) and stock in 12 aquaria (13L). Three concentrations of F. carica leaf extract (0.3g/L, 0.5g/L and 0.7g/L) were used as the treatment and the control is 0g/L. Each treatment is in triplicate. Water parameters such as dissolved oxygen, pH and temperature of the treatment tank were in the range of (4.05 – 4.20 ppm), (7.3 – 8.0) and (25.6 – 28.8⁰C) respectively. The potential of the extract as a treatment agent in the infected fish was recorded in term of fish survival and physical condition such as the sign of bacterial infection and swimming behaviour. The results showed disease tilapia treated with F. carica leaf extract (0.7g/L) possess the highest survival rate at day 5 (67%) and remain until day 15. Compared to the survival rate of 0%, 33% and 53% for the concentration F. carica leaf extract 0g/L, 0.3g/L, and 0.5g/L respectively. Treatment with higher concentration of F. carica leaf extract showed improvement in physical condition and swimming behaviour of disease Tilapia. It could be concluded that F. carica leaf extract has potential in treatment of A. hydrophila associate disease in Fish. Keywords: Ficus carica, Aeromonas hydrophila, bioassay, Oreochromis niluticus, in vivo * Corresponding author Name: Norashikin Binti Anjur Address: Department of Technology and Natural Resources, Faculty of Applied Sciences and Technology (FAST), Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Educational Hub, 84600 Pagoh, Muar, Johor, Malaysia Tel: 017-4897946 Email: norashikinanjur@gmail.com
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 40 Oral presentation 13 Effect of Organic Pesticide in Controlling Pest and Disease in Capsicum Cultivation I. Norahim1* 1Faculty of Science, Department of Biosciences, Universiti Teknologi Malaysia, 81310 Abstract One of the plant pathogens responsible for diseases in chilli cultivation is anthracnose. Anthracnose disease infected by Colletricum species is one of the diseases that contributed vastly to the low yield of crop production. Using bio-agricultural practices, neem extract has become one of the promising antifungal agent in combating Anthracnose and also an alternative to chemical approach, and was conducted via in vivo and vitro. For in vivo, the effectiveness of organic pesticides was evaluated via foliar application. Neem oil and combined application of neem, garlic and chilli at different concentrations were sprayed at chilli plants and the results were examined 2 months after the treatment. For in vitro, the efficacy was carried out using food poisoned technique. Above all, pathogens were isolated from infected leaves. Isolation resulted in 3 different colonies; presumably characterized as C. capsici, C. gloeosporiodes, and C. truncatum. For foliar application, treated plants showed less defoliation of infected leaves and newly formed leaves and fruits showed fewer symptoms of Anthracnose disease and less occurrence of insect biting post foliar application compared to that of before. Through poisoned food technique, the highest percentage inhibition of mycelial growth of Colletotrichum sp. was recorded by NGC (1.5%) as compared to neem oil and NGC with varied concentrations as well as for control. These results conclude that application of organic pesticide is effective in reducing densities of pests for agricultural activities. Keywords: Chilli, plant pathogen, anthracnose, neem, organic pesticide * Corresponding author Name: Dr Norahim Bin Ibrahim Address: Faculty of Science, Department of Biosciences, Universiti Teknologi Malaysia. Tel: 07-5557544 Email: norahim@utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 41 Oral presentation 14 Phenotypic and Genetic Variation of Capsicum annuum Germplasm Collection Zulaikha S.1*, Sarmidi M.R.1, & Rafii M.Y.2 1Innovation Centre in Agritechnology for Advanced Bioprocessing (ICA) Universiti Teknologi Malaysia Pagoh 2Faculty of Chemical and Energy Engineering (FCEE), Universiti Teknologi Malaysia 3Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia Abstract Capsicum annuum is widely cultivated in Malaysia. However, production of the local chili varieties is low and very prone to insects and diseases. Breeding program will be a complementary study to insects and diseases study and agronomic practices. Introduction and introgression of new germplasm from abroad for local environment adaptability is very important for development of new superior varieties. This study aimed to estimate genetic diversity and heritability values of 30 genotypes of C. annum collected from Asian Vegetable Research and Development Center (AVRDC) and to identify potential superior accessions with high yield potential, and disease and insect resistant for future breeding program. The Capsicum annuum genotypes were characterized for days to flowering (DF), days to maturity (DM), plant height (PH), fruit yield (FY), fruit length (FL), number of fruit per plant (NFL), leaf area (LA) and disease score (DS). The data of these traits were subjected to the analysis of variance (ANOVA), heritability component and correlation analysis, and multivariate analysis using cluster analysis based on percent similarity coefficient. Results showed significant differences for all the traits except on DM and PH at week 8, 10, and 12. High heritability values (> 60%) were observed for DF, FY, FL, DS, LA and, PH at weeks 2 and 4. Through cluster analysis, Cluster III (AVPP0012, AVPP0904, AVPP9905, and AVPP9813) was the best cluster as compared to others. In addition, cluster VII (AVPP 0804) was highly tolerance to mosaic disease. The genotypes in Cluster III had good performance in FY, NFL, DS and LA while Cluster VII for disease score. These potential genotypes can be recommended for future breeding program. Keywords: Genotypes, genetic diversity, Capsicum annuum, heritability component analysis, cluster analysis * Corresponding author Name: Zulaikha binti Sarobo Address: Innovation Centre in Agritechnology for Advanced Bioprocessing (ICA), Universiti Teknologi Malaysia Pagoh, Jalan Edu Hub UTM 2, Hub Pendidikan Tinggi Pagoh, 84600 Pagoh, Johor Darul Takzim Tel: 06-9742854 Email: zulaikha-s@utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 42 Oral presentation 15 Control and monitoring system for livestock feeding time via smartphone K. Aina1, J.S. Eik1, U. Nasrah1, M.A. Ilham1, N.A. Fakharulrazi2, & F. Yakub2* 1 Department of Electronic System Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia 2Wind Engineering for Environment Laboratory, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia Abstract The livestock farming sector usually requires a lot of manpower and may face with problems such as adhering the designated feeding schedule. Therefore, this paper intends to aid in this sector by bringing the Smart Feeder System. The main objective of this paper is to control and monitor livestock feeding time using application via smartphones. This is to ensure that the livestock are fed according to the right schedule. This paper also highlighted the advantage of the Smart Feeder System where it can be used to save up money and energy that can be obtained by having less labor workers. This is because of the hopper storage that can accommodate the food up to three days. The importance of this system is its ability to automate the feeding system for the livestock. The Smart Feeder System designed is different from the existing livestock feeding machines that are available anywhere in the market due the implementation of Internet of Things into the system. The usage of Internet of Things allows farmers to set the feeding schedule automatically by using application via smartphone. Besides, users will also be informed on the weight of food in the storage and food container through the application. This will allow the users to monitor the food storage and only come to refill it when it is empty. Farmers can also observe whether the livestock have eaten or not by getting updated on the food level in the level in the food storage using ultrasonic sensor. Keywords: Smart Feeder System, livestock farming, Internet of Things * Corresponding author Name: Fitri Yakub Address: Wind Engineering for Environment Laboratory, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia Tel: 03-22031286 Email: mfitri.kl@utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 43 Oral presentation 16 Building a smart gardening system and plant monitoring using IoT H.S. Miin1, S. Maria1, M.H. Aizam1, M.S. Fauzan1, N.A. Fakharulrazi2, & F. Yakub1,2* 1Department of Electronic System Engineering, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia 2Wind Engineering for Environment Laboratory, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia Abstract Gardening activities usually required a lot of time and gardeners may face varieties of problems such as sticking to the designated watering schedule. Thus, this paper intends to provide a way to solve the risen problems or a way out for the gardeners to deal with this situation by introducing the Smart Gardening System. By using this system, users will be able to control and monitor the watering schedule and the sufficiency of lights while ensuring that the plants are taken care of accordingly. The smart gardening system is different from the normal gardening products that are already available in the market because of the implementation of Internet of Things (IoT) in the system to facilitate the work for its users. By using this home based system, user can set the watering and lighting schedule automatically by using the designated application via smartphone. Besides, users will also be notified on the moisture level of soil, light - exposure and the water level in water tank through the application. This will allow the users to monitor the watering system and only come to refill it when the water tank is empty. This system is at advantage as it can run automatically. From this research, the benefit of smart gardening system is proven via the execution of IoT which requires less human intervention for the system to operate and also the usage of all kind of sensors that highlighted the problems faced by gardeners facilitate the work required. Keywords: Internet of Things, smart gardening system, plant monitoring, prototype, sensors * Corresponding author Name: Fitri Yakub Address: Wind Engineering for Environment Laboratory, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia Tel: 03-22031286 Email: mfitri.kl@utm.my
2 n d I C A R E S E A R C H S Y M P O S I U M 2 0 1 9 | 44 Oral presentation 17 Quality Assessment of Meats Using FT-IR Spectroscopy, Texture Analyzer, Color Spectrophotometer and Image Analysis Ainur Nalisa Abd Rashid1, Saliza Asman2* 1Department of Food Technology and Natural Resources, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia, UTHM Pagoh Campus, Pagoh Higher Education Hub, KM 1, Jalan Panchor, 84600 Muar, Johor, Malaysia. 2Department of Physics and Chemistry, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia, UTHM Pagoh Campus, Pagoh Higher Education Hub, KM 1, Jalan Panchor, 84600 Muar, Johor, Malaysia. Abstract Rapid growth in consumer demand for meat has increased and become focused attention on maintaining meats safety and quality. However, improper handling and storage of meats may cause unfavorable feature on meats as it is a highly perishable food with short shelf-life. The implementation of rapid and non-destructive quality assessment method of meat have become increasingly important to satisfy consumer demand. This study implements such method by using Fourier Transform Infrared Spectroscopy (FT-IR), color spectrophotometer, texture analyzer and image analysis to classify meats of different types and their shelf-life during freezing temperature storage (0˚C) for five days in a row. Three types of common commercial meats (chicken, cow and goat’s meat) with boiling process were selected. The FT-IR analysis revealed presence of functional group that represents absorbance bands of proteins, triglycerides, fatty acids and carbohydrates with different intensities according to types of meat, type of processing (boiled) and days of storage. Freezing storage caused slightly alterations for the color parameters and partial degradation of some proteins in meat. The texture analysis also shows significant different in all of the samples with different types of meat and boiling method and the image analysis shows significant degradation of textural surface of the meat with increasing storage period. Keywords: Meats, FTIR, texture analysis, color spectrophotometer, image analysis * Corresponding author Name: Dr. Saliza binti Asman Address: Department of Physics and Chemistry, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia, UTHM Pagoh Campus, Pagoh Higher Education Hub, KM 1, Jalan Panchor, 84600 Muar, Johor, Malaysia Tel: 06-974 5129 Email: salizaa@uthm.edu.my
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