18TH ANNUAL IFATS CONFERENCE - FORT LAUDERDALE - FLORIDA - November 18-20, 2021
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International Federation for Adipose Therapeutics and Science
18TH ANNUAL IFATS CONFERENCE
FORT LAUDERDALE - FLORIDA
November 18-20, 2021
THERE’S ONLY ONE IFATS - IFATS.ORG
18th Annual IFATS Conference N ovem ber 18- 20, 2021
Presidential Note
On behalf of the IFATS Board of Directors, I have the pleasure to welcome
you to our 18th Annual Meeting in beautiful Ft. Lauderdale, Florida.
In 2002, IFATS was founded by four scientific visionaries following the historical discovery
of mesenchymal stem cells in human subcutaneous adipose tissue. Since then, the IFATS
annual meeting has presented leading professionals in the exciting field of regenerative
medicine with the opportunity to both present and learn about cutting edge scientific and clinical research.
A robust group of plastic surgeons, cell biologists, research scientists and physicians in other complementary
domains gather at this meeting each year where they exchange the most current knowledge on basic,
translational, and clinical research in adipose-derived products, including adipose-derived stem cells (ASC’s).
IFATS works closely with other leading scientific organizations to organize collaborating panels for attendees that
will be an important part of our 18th annual event. We will once again present the Industry Showcase, and this
year it will take part of our podium presentations. In addition, several of our industry leaders will offer “Lunch and
Learn” Tables available with reserved seating on Thursday.
The IFATS annual meeting provides all our attendees the opportunity to learn about state-of-the-art technology
and clinical practice, as well as, cutting-edge products developed by our exhibiting companies, and further, to
interact with the brightest minds in the field. For the first time, this year will be offered as a hybrid meeting. Those
who cannot attend due to travel restrictions or schedules may join us “Live” on line streaming. The 2021 meeting
features a Thursday evening casual gathering on the hotel pool deck overlooking beautiful Fort Lauderdale beach
during which attendees will enjoy time with colleagues and a chance to interact with international leaders in the
field.
We are very pleased that you will join us at the IFATS annual meeting this year and we are sure you will benefit
from exciting new ideas and valuable tools for your research and clinical practice.
Ivona Percec, MD, PhD
IFATS President
2 For t Lauderdal e, Fl ori da
N ove mb er 18- 20, 2 0 2 1 18th Annual IFATS Conference
Executive Committee - Board of Directors
Executive Committee
William Futrell, MD Adam J. Katz, MD Ramon Llull, MD, PhD Ricardo Rodriguez, MD J. Peter Rubin, MD
Pittsburgh, PA, USA Past President 2005 Past President 2003 Past President 2016 Past President 2004
Winston-Salem, NC, USA Winston-Salem, NC, USA Baltimore, MD, USA Pittsburgh, PA, USA
Board of Directors
Torsten Blunk, PhD Ivona Percec, MD, PhD Guy Magalon, MD Kotaro Yoshimura, MD Bruce Bunnell, PhD
President-Elect 2022 President 2020-2021 Past President 2019 Past President 2018 Past President 2015
Wurzburg, Germany Bryn Mawr, PA, USA Marseille, France Shimotsuke, Japan New Orleans, LA, USA
Marco Helder, PhD Kacey Marra, PhD Sydney Coleman, MD Julie Fradette, PhD Stuart K. Williams, PhD
Past President 2014 Past Co-President 2013 Past Co-President 2013 Past President 2012 Past President 2011
Amsterdam, Netherlands Pittsburgh, PA, USA New York, NY, USA Quebec, QC, Canada Louisville, KY, USA
Louis Casteilla, PhD Keith March, MD, PhD Jeffrey Gimble, MD, PhD
Past President 2008 Past President 2007 Past President 2006
Toulouse, France Gainesville, FL, USA New Orleans, LA, USA
Fort L au d erd al e, F lo r id a 3
18th Annual IFATS Conference N ovem ber 18- 20, 2021
Scientific Program Committee
Torsten Blunk, PhD Ivona Percec, MD, PhD
Bruce Bunnell, PhD Ricardo Rodriguez, MD
Jeffrey M. Gimble, MD, PhD J. Peter Rubin, MD
Guy Magalon, MD
Invited Speakers & Session Moderators
Rosalyn Abbott, PhD Farshid Guilak, PhD Doug Oliver, MSW
Katarina Andjelkov, MD, PhD Marco Helder, PhD Kentaro Onishi, DO
Fabrizio Bembo, PhD LaTonya Hickson, PhD Graham Parker, PhD
Mark Berman, MD Aaron W. James, MD Ivona Percec, MD, PhD
Torsten Blunk, PhD Adam J. Katz, MD Robert Rehnke, MD
Robert Bowen, MD, FCCP, RVPI Roger Khouri, MD Kyle Richardson
Bruce Bunnell, PhD Lauren Kokai, MD Camilo Ricordi, MD
Bill Cimino, PhD Fred Kolle, MD, PhD Ricardo Rodriguez, MD
Sherry Collawn, MD, PhD Benjamin Levi, MD J. Peter Rubin, MD
Jason Dragoo, MD Ramon Llull, MD, PhD Gordon H. Sasaki, MD
Jaime Garza, MD Marc Long, PhD Bernard Siegel, MD
Alexandra Conde-Green, MD Mandi J. Lopez, DVM, MS, PhD, DACVS Filip Stilaert, MD
Julie Fradette, PhD Jeremy Magalon, MD Stuart Williams, PhD
Trivia Frazier, PhD Keith March, MD Kotaro Yoshimura, MD
Pietro Gentile, MD Kacey Marra, PhD
Jeffrey M. Gimble, MD, PhD Susanna Meitienen, PhD
Abstract Reviewers
Rosalyn Abbott Alexandra Conde-Green, MD Malgorzata Kolend, PhD
Carnegie Mellon University Private Practice Klinika Kolasinski
Katarina Andjelkov, MD, PhD Julie Fradette, PhD Fred Kolle, MD
University of Belgrade & BelPrime Clinic LOEX-Universite Laval Aleris-Hamlet Hospitals
Petra Bauer-Kreisel, PhD Trivia Frazier, PhD Kacey Marra, PhD
University of Wuerzburg Obatala Sciences University of Pittsburgh
Torsten Blunk, PhD Jeffrey M. Gimble, MD, PhD Susanna Mietienen, PhD
University of Wuerzburg LaCell LLC and Obatala Sciences Inc. University of Tampere
Robert Bowen, MD, FCCP, RVP Martin Harmsen, PhD Nir Shani, PhD
Private Practice University Medical Centre Groningen Tel Aviv Sourasky Medical Center
Bruce Bunnell, PhD Jeffrey Hartog, MD, DMD Sugii Shigeki, PhD
University of North Texas Health Science Center Private Practice Singapore Bioimaging Consortium / Duke-NUS Graduate
at Fort Worth Medical School
Marco Helder, PhD
Louis Casteilla, PhD VU University Medical Center Filip Stillaert, MD
University of Toulouse University Hospital Ghent
Susanna Kauhanen, MD, PhD
Evangelia Chnari, PhD Helsinki University Hospital Dmitry Traktuev, PhD
MTF Biologics University of Florida College of Medicine
Paul Kingham, PhD
Sherry Collawn, MD, PhD Umeå University Stuart Williams,PhD
University of Alabama at Birmingham University of Louisville
Lauren Kokai, MD
University of Pittsburgh
4 For t Lauderdal e, Fl ori da
N ove mb er 18- 20, 2 0 2 1 18th Annual IFATS Conference
INNOVATION IS A THING OF BEAUTY
A start-to-finish closed An off-the-shelf allograft
system to streamline alternative to small
fat grafting procedures volume fat grafting
mtfbiologics.org | @mtfbiologics MTF Biologics is proud to be a Gold Sponsor of IFATS.
Fort L au d erd al e, F lo r id a 5
18th Annual IFATS Conference N ovem ber 18- 20, 2021
• THURSDAY - NOVEMBER 18
7:00 - 8:00 am Continental Breakfast in Exhibit Hall Los Olas Foyer
Welcome Remarks and Overview
8:00 - 8:30 am Ivona Percec, MD, PhD - IFATS President
Los Olas Ballroom
Keynote Speaker - Jeremy Magalon, MD
8:30 - 9:15 am The (r)evolution of Point of Care Regenerative Medicine Los Olas Ballroom
Moderator: Ivona Percec, MD, PhD
Guest Speaker - Graham Parker, PhD, Stem Cells and Development Journal Editor
9:15 - 9:30 am Moderator: Jeffery M. Gimble, MD, PhD
Los Olas Ballroom
Plenary Session 1 - BEST PAPERS (Award Eligible)
9:30 - 10:30 am Moderator: Torsten Blunk, PhD and Bruce Bunnell, PhD Los Olas Ballroom
1 - MESENCURE—AN ENHANCED ALLOGENEIC ADIPOSE-DERIVED CELL THERAPY DEVELOPED FOR TREATING ACUTE RESPIRATORY
DISTRESS IN COVID-19: FROM BENCHTOP TO BEDSIDE
9:38 am Presenter: Tomer Bronshtein (Israel)
Affiliation: Bonus BioGroup Ltd.
Authors: BEN DAVID D; NOVAK A; KIVITY V; HAMOUD S; HAYEK T; MERETZKI
2 - DEVELOPMENT OF A 3D BIOPRINTED BREAST CANCER-STROMA CO-CULTURE MODEL UTILIZING ADIPOSE-DERIVED STROMAL
CELL SPHEROIDS
Presenter: Hannes Horder (Germany)
9:46 am Affiliation: University of Wuerzburg
Authors: GUAZA LASHERAS M; GRUMMEL N; NADERNEZHAD A; HERBIG J; ERGUN S; TESSMAR J, GROLL J; FABRY B; BAUER-KREISEL
P, BLUNK T J
3 - VASCULARIZED ADIPOSE TISSUE GRAFT USING A DECELLULARIZED LUNG FOR SOFT TISSUE RECONSTRUCTION
Presenter: Rosalyn Abbott (USA)
9:54 am Affiliation: Carnegie Mellon University
Authors: DEBARI MK; NG WH; KOKAI LE; MARRA KG; RUBIN JP; REN X
4 - ADIPOSE STEM CELL-BASED THERAPIES FOR FIBROSIS AND SCARRING OF THE VULVA ADIPOSE STEM CELL-BASED THERAPIES FOR
FIBROSIS AND SCARRING OF THE VULVA
10:02 am Presenter: Aurora Almadori (Italy)
Affiliation: UCL - Royal Free Hospital
Authors: BOYLE D; HANSEN E; REID W; MACLEAN A; BUTLER PE
5 - CHANGES IN EXPANSION PRESSURES IN EXPANDER-BASED POST-MASTECTOMY DEFECT RECONSTRUCTION: THE EFFECT OF FAT
GRAFTING IN NORMAL AND RADIATED TISSUES
10:10 am Presenter: Donald Browne (USA)
Affiliation: Wake Forest Baptist Health
Authors: MONSERRAT J; MATAS A; SESE B; LLULL R
6 - STROMAL VASCULAR FRACTION MITIGATES RADIATION-INDUCED GASTRO-INTESTINAL SYNDROME
Presenter: Lydia Bensemmane (France)
10:20 am Affiliation: IRSN
Authors: SQUIBAN C; DEMARQUAY C; MATHIEU N; BENDERITTER M; MILLIAT F; LINARD C
10:18 - 10:30 am Discussion Los Olas Foyer
10:30 - 11:00 am Coffee Break in Exhibit Hall Los Olas Ballroom
Free Papers 1 - Exosomes
11:00 - 12:00 pm Moderators: Ramon Llull, MD, PhD, & Marco Helder, PhD
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7 - IMMUNOMODULATORY AND REGENERATIVE EFFECTS OF THE FULL AND FRACTIONED ADIPOSE TISSUE DERIVED STEM CELLS
SECRETOME IN SPINAL CORD INJURY
Presenter: Antonio Salgado (Portugal)
11:00 am Affiliation: University of Minho
Authors: CIBRÃO J.; PINHOE A.; LIMA R; GOMES E; SERRA S; LENTILHAS-GRAÇA J; RIBEIRO C; LANCEROS-MENDEZ S; TEIXEIRA F;
MONTEIRO S; SILVA N
8 - WHARTON’S JELLY MSC-DERIVED SMALL EXTRACELLULAR VESICLES: A NATURALLY NANOTHERAPEUTIC AGENT AMELIORATES
OSTEOARTHRITIS BY CARRYING MIRNA CARGO
11:10 am Presenter: Penghong Chen (China)
Affiliation: Fujian Medical University Union Hospital
Authors: CHEN XS
9 - MESENCHYMAL STEM CELL-DERIVED EXTRACELLULAR VESICLES IN AUTOLOGOUS FAT GRAFTING – LESSONS FROM A
METHODOLOGICAL ANALYSIS
11:20 am Presenter: Mohammad Ghiasloo (Belgium)
Affiliation: Ghent University
Authors: DE WILDE L; SINGH K; TONNARD PL; VERPAELE A; DE WEVER O; HENDRIX A; BLONDEEL PN
10 - INVESTIGATING THE EFFECT OF FACTORS SECRETED FROM ADIPOSE TISSUE ON MYOFIBROBLAST DIFFERENTIATION
Presenter: Samuel Higginbotham (United Kingdom)
11:30 am Affiliation: The University of Sheffield
Authors: WORKMAN VL; GREEN NH; GIBLIN V; LAMBERT DW; HEARNDEN
11 - HUMAN PLATELET LYSATE CONCENTRATION IN CULTURE MEDIA AFFECT ADIPOSE-DERIVED STROMAL/STEM CELL POTENCY
Presenter: Jesper Svalgaard (Denmark)
11:40 am Affiliation: Stemmedical
Authors: BROOKS P; BALLESTEROS O; MUNTHE-FOG L; FISCHER-NIELSEN A; HAASTRUP E
12 - DEVELOPMENT OF AN ORGANOID MODEL DERIVED FROM HUMAN ADIPOGENIC STEM/PROGENITOR CELLS TO STUDY WHITE
ADIPOSE TISSUE PHYSIOLOGY
Presenter: Markus Mandl (Austria)
11:50 am Affiliation: Research Institute for Biomedical Aging Research
Authors: VIERTLER HP; BRUCKER C; HATZMANN FM; WALDEGGER P; RAUCHENWALD T; MATTESICH M; ZWIERZINA M; PIERER G;
ZWERSCHKE W
12:00 - 1:00 pm Lunch & Learn Tables - Stem Cell & Development, MTF Biologics, Obatala Sciences Los Olas Ballroom
Guest Speaker - Gordon H. Sasaki, MD
1:00 - 2:00 pm PRP, Exosomes & Fat Hair Folicle Growth Los Olas Ballroom
Moderator: Katarina Andjelkov, MD, PhD
Industry Showcase
2:00 - 3:00 pm Moderators: Marc Long, PhD and Bill Cimino, PhD
Los Olas Ballroom
Scaling up the Revolve ™ Platform: Introducing Science and Performance Data for REVOLVE ENVI ™ 600
2:00 pm Presenter: Maryellen Sandor, PhD, Associate Director, Biological Research
Affiliation: Allergan Aesthetics an AbbVie company
Fat Quality Using a Start-to-Finish Closed Autologous Grafting System (LipoGrafter®)
2:10 pm Presenter:Anouska Dasgupta
Affiliation: MTF Biologics
Your Website: Does It Trigger Scrutiny By Enforcement Agencies?
2:20 pm Presenter: Douglas Oliver, MSW
Affiliation: Regenerative Outcomes
Designing Adipogenic Microphysiological Systems with ObaGel
2:30 pm Presenter: Trivia Frazier, PhD
Affiliation: Obatala Sciences, Inc.
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18th Annual IFATS Conference N ovem ber 18- 20, 2021
Microfragmented Fat:Methods, Mechanisms and Regulations
2:40 pm Presenter: Nathan Katz
Affiliation: Jointechlabs
Autologous Fat Grafting Accelerates Healing of Recalcitrant Chronic Ulceration on the Diabetic Foot,
Non-healing Pressure Sores and Other Problem Wounds of the Lower Limb
2:50 pm Presenter: Tilman Stasch, MBChB, MRCS(Ed.), F EBOPRAS, MD
Affiliation: CAREstream America
3:00 - 3:30 pm Coffee Break in Exhibit Hall Los Olas Foyer
Free Papers 2
3:30 - 4:30 pm Moderators: Jeremy Magalon, MD & Robert Bowen, MD FCCP, RVPI
Los Olas Ballroom
13- SUPERCHARGED MECHANICAL STROMAL-CELL TRANSFER (MEST) : COMBINATION OF PPP AND CONDENSED FAT, ADINIZING,
AND PRP ADDITION
3:30 pm Presenter: Sule Oztan (Turkey)
Affiliation: Stem-bio Cell and Tissue technology
Authors: COPCU HE
14 - HUMAN-PLATELET LYSATE SERUM AS A POTENTIAL CLINICAL-TRANSLATABLE SUPPLEMENT TO SUPPORT HUMAN ADIPOSE-
DERIVED STEM CELLS NEUROTROPHIC PROPERTIES
3:40 pm Presenter: Martino Guiotto (Switzerland)
Affiliation: Centre Hospitalier Universitaire Vaudois - CHUV
Authors: PALOMBELLA S; HIGGINS G; APPLEGATE L; RAFFOUL W; HART A; CHERUBINO M; RIEHLE M; DI SUMMA P
15 - UPDATED PLATELET-RICH PLASMA UNDERSTANDINGS AND THERAPEUTIC CONSIDERATIONS
Presenter: Peter Everts (USA)
3:50 pm Affiliation: Gulf Coast Biologics
Authors: FABIO LANA J; MAUTNER K; ONISHI K; JAYARAM P
16 - AUTOLOGOUS MICRO-FRAGMENTED ADIPOSE TISSUE (LIPOGEMS): THE ROLE OF MESENCHYMAL STEM CELLS FOR THE
TREATMENT OS RECURRENT PERIANAL FISTULAS. MULTICENTRIC - (BRAZIL - ITALY)
4:00 pm Presenter: Ana Luiza Silva (Brazil)
Affiliation: Universidade de Mogi das Cruzes
Authors: ROTTA CM; ELBETTI C; GIANI I; BURG MB; TRAGO S
17 - INVESTIGATION INTO USE OF SOLUBLE AND SUSTAINED RELEASE LATANOPROST FOR REDUCING ADIPOSE VOLUME
Presenter: Matthew Cannon (USA)
4:10 pm Affiliation: University of Pittsburgh
Authors: SUKINIK J; LEE P; GUERRERO D; SEMAN S; NERONE WV; LODER S; SU S; KOKAI LE
18 - HORMONAL, ADIPOGENIC AND ANGIOGENIC ALTERATIONS IN LIPEDEMA ADIPOSE TISSUE
Presenter: Eleni Priglinger (Austria)
4:20 pm Affiliation: Ludwig Boltzmann Institute Traumatology
Authors: HOFMANN M; STROHMEIER K; JACAK J; WEIGL M; HACKL M; HOLNTHONER W; GRILLARI J; REDL H; SANDHOFER M;
WOLBANK S
Panel 1 - Clinical Applications in Soft Tissue Reconstruction
4:30 - 5:30 pm Moderators: Alexandra Conde-Green, MD and Roger Khouri, MD Los Olas Ballroom
Panelists: Roger Khouri, MD; Robert D. Rehnke, MD; Ricardo Rodriguez, MD
Guest Speaker - Farshid Guilak, PhD
5:30 - 6:30 pm Osteoarthritis and Fat: The Good, The Bad, and The Ugly Los Olas Ballroom
Moderator: Jeffrey M. Gimble, MD, PhD
6:30 pm Meeting Adjourns for the day
Conference Reception & Dinner - Hotel Pool Terrace
7:00 - 9:00 pm Pre-registration is required
8 For t Lauderdal e, Fl ori da
N ove mb er 18- 20, 2 0 2 1 18th Annual IFATS Conference
• FRIDAY - NOVEMBER 19
Continental Breakfast in Exhibit Hall
7:00 - 8:00 am Los Olas Foyer
Informal Committee Meetings
Plenary Session 2 - Regenerative Therapies for Orthopedics &
Sports Medicine
8:00 - 9:30 am Moderators: J. Peter Rubin,MD and Mandi J. Lopez, DVM, MS, PhD, DACVS Los Olas Ballroom
Panelists: Kentaro Onishi, DO; Jaime Garza, MD; Farshid Guilak, PhD; Jason Dragoo,
MD; Kyle Richardson
Main Auditorium - Los Olas Ballroom Room 2 - Bonnet
Free Papers 7 - Clinical Topics II
9:30 - 10:30 am Moderators: Sherry Collawan, MD, PhD & LaTonya
Hickson, PhD
46 - VALIDATION OF A NOVEL, SIMPLE AND
INEXPENSIVE SCANNING PROCESS FOR THE THREE-
DIMENSIONAL ASSESSMENT OF THE GLUTEAL REGION
9:30 am Presenter: Carlo Oranges (Switzerland)
Affiliation: Basel University Hospital
Authors: RICKLIN A; BENITEZ B; SCHARMA N; SCHAEFER
DJ; KALBERMATTEN DF; THIERINGER FM
47 - ENRICHMENT OF THE FACIAL FAT GRAFT FOR
INCREASED VOLUME RETENTION, A SYSTEMATIC
REVIEW
9:40 am Presenter: Jan Aart Schipper (Netherlands)
Affiliation: University Medical Centre Groningen
Authors: VRIEND L; TUIN AJ; DIJKSTRA PU; SCHEPERS RH;
VAN DER LEI B; JANSMA J; HARMSEN MC
Panel 2 - Advances in Bone Regeneration
Moderators: Marco Helder, PhD and
48 - ENHANCED UTILITY AND OPERATIVE EFFICIENCY
9:30 - 10:30 am Susanna Meitienen, PhD
OF THE NOVEL PUSH-TO-SPIN HANDHELD (P2S) FAT
Panelists: Marco Helder, PhD; Susanna Meitienen, PhD;
GRAFT PROCESSING DEVICE
Fabrizio Bembo, PhD; Benjamin Levi, MD
9:50 am Presenter: Shawn Loder (USA)
Affiliation: University of Pittsburgh
Authors: LEE PL; KOKAI L; RUBIN JP; GUSENOFF BR;
GUSENOFF JA
49 - MSC MECHANICAL DISSOCIATION SINCE 2006:
THE PARADIGMA SHIFT AND THE NEW CLINICAL
10:00 am APPLICATIONS
Presenter: Hebert Lamblet (Brazil)
Affiliation: Vikaara Klinik
50 - DIODE LASER ASSISTED FAT GRAFT AND
REGENERATIVE SURGERY: 3 YEARS OF EXPERIENCE
Presenter: Elena Fasola (Italy)
10:10 am Affiliation: IRCCS FONDAZIONE CA GRANDA OSPEDALE
MAGGIORE
Authors: LOMBARDI B; CAPRETTI A; LAZZARI L
10:20 am
Fort L au d erd al e, F lo r id a 9
18th Annual IFATS Conference N ovem ber 18- 20, 2021
10:30 - 11:00 am Coffee Break in Exhibit Hall Los Olas Foyer
Main Auditorium - Los Olas Ballroom Room 2 - Bonnet
Free Papers 8 Musculoskeletal Applications
11:00 - 12:30 pm Free Papers 3 - Cell Activity and Senescence 11:00 - 12:30 pm Moderators: Susanna Miettinen, PhD
Moderators: Lauren Kokai, MD & Trivia Frazier, PhD
& Fred Kølle, MD, PhD
19 - QUIESCENCE, STEMNESS AND ADIPOGENIC 52 - BONOFILL FROM BENCH TO BEDSIDE: A NOVEL
DIFFERENTIATION CAPACITY IN HUMAN DLK1-/CD34+/ TISSUE-ENGINEERED PRODUCT GENERATED FROM
CD24+ ADIPOSE STEM/PROGENITOR CELL ADIPOSE-DERIVED MESENCHYMAL STROMAL CELLS
Presenter: Florian Hatzmann (Austria) IN LINE TO REPLACE BONE AUTOGRAFTS FOR LARGE
11:00 am Affiliation: University of Innsbruck 11:00 am SEGMENTAL BONE DEFECT APPLICATIONS
Authors: EJAZ A; WIEGERS GJ; MANDL M; BRUCKER Presenter: Atara Novak (Israel)
C; LECHNER S; RAUCHENWALD T; ZWIERZINA M; Affiliation: Bonus BioGroup LTD.
BAUMGARTEN S; WAGNER S; MATTESICH M; WALDEGGER Authors: NOVAK A; BRONSHTEIN T; KIVITY V; TZUR E;
P; PIERER G; ZWERSCHKE W ROZEN N; MERETZKI S
53 - INTRA ARTICULAR INJECTION OF AUTOLOGOUS
MICROFAT AND PLATELET-RICH PLASMA IN THE
TREATMENT OF KNEE OSTEOARTHRITIS: A DOUBLE
20 - SENESCENCE DID NOT ALTER THE
BLIND RANDOMIZED COMPARATIVE STUDY
CHONDROPROTECTIVE EFFECT OF EXTRACELLULAR
Presenter: Jeremy Magalon (France)
VESICLES FROM ADIPOSE MESENCHYMAL STROMAL CELLS
11:10 am Presenter: Jérémy Boulestreau (France)
11:10 am Affiliation: Culture and Cell Therapy Unit INSERM
CBT1409 C2VN Research Unit
Affiliation: Inserm
Authors: LOUIS ML; GRAVIER DUMONCEAU R; JOUVE
Authors: NOEL D; MAUMUS M; ROZIER P; JORGENSEN C
E; COHEN M; DJOURI R; RICHARDET N; JOURDAN
E; GIRAUDO L; DUMOULIN C; GRIMAUD F; DIGNAT
GEORGE F; VERAN J; SABATIER F; MAGALON J
54 - A NOVEL METHOD TO PROVIDE 3D MRI IMAGING
21 - DETECTION, QUANTIFICATION AND ELIMINATION
FOR EVALUATING HUMAN KNEE CARTILAGE: CLINICAL
OF SENESCENCE IN AGED AND/OR CULTURE EXPANDED
TRAIL OF SVF THERAPY FOR HUMAN KNEE OA
HUMAN ADIPOSE DERIVED STEM CELLS
Presenter: Xin Xiao Zheng (China)
11:20 am Presenter: Sudheer Ravuri (USA) 11:20 am Affiliation: Wuhan University Zhongnan Hospital
Affiliation: Steadman Philippon Research Institute
Authors: LIU R; REN B; XIAO F; XU J; LI L; LI T; RUAN Z;
Authors: MULLEN MM; BILLINGS JB; MITCHELL JM;
BAO Y; LING J; ZHAO J; LIAO W; PAN Z; RUBIN P; XU H;
HAMBRIGHT SH; HUARD JH
TIAN J; CAI L
55 - IMMUNOREGULATORY PROPERTIES OF A HUMAN
22 - ADIPOSE DERIVED STEM CELLS ACCUMULATE
LYOPHILIZED 3D SCAFFOLD FREE TISSUE ENGINEERED
SENESCENCE WITH AGE AND SERIAL PASSAGING
PRODUCT FOR BONE RECONSTRUCTION
11:30 am Presenter: Michael Mullen (USA) 11:30 am Presenter: Carmen Brenner (Belgium)
Affiliation: Steadman Philippon Research Institute
Affiliation: Novadip Biosciences SA
Authors: HUARD J; HAMBRIGHT WS; RAVURI S
Authors: EPISKOPOU H
56 - BONOFILL: A NOVEL TISSUE-ENGINEERED
23 - IDENTIFICATION AND HISTOLOGICAL MAPPING OF AUTOLOGOUS BONE GRAFT FROM ADIPOSE-DERIVED
SENESCENT STROMAL CELLS IN ADIPOSE TISSUE: A PATH MESENCHYMAL STROMAL CELLS FOR MAXILLOFACIAL
TOWARDS TISSUE DESENESCENCE BONE TISSUE REGENERATION
11:40 am Presenter: Anthony Elias (USA)
11:40 am Presenter: Atara Novak (Israel)
Affiliation: Wake Forest Baptist Medical Center Affiliation: Bonus BioGroup LTD.
Authors: JUSTICE JN; LLULL R Authors: BEN-DAVID D; BRONSHTEIN T; ROZEN N; KIVITY
V; TZUR E; MERETZKI S
10 For t Lauderdal e, Fl ori daN ove mb er 18- 20, 2 0 2 1 18th Annual IFATS Conference
Main Auditorium - Los Olas Ballroom Room 2 - Bonnet
24 - INVOLVEMENT OF STROMAL AGE IN BREAST CANCER 57 - MECHANICALLY DERIVED TISSUE-STROMAL
ENDOCRINE RESPONSE VASCULAR FRACTION ACTS ANTI-INFLAMMATORY ON
Presenter: Katie Hamel (USA) CHONDROCYTES IN VITRO
11:50 am Affiliation: Louisiana State University 11:50 am Presenter: Lucienne Vonk (Netherlands)
Authors: CAVALIER MB; LIIMATTA KQ; ROZANSKI GL; KING Affiliation: University Medical Center Groningen
CT; BELGODERE JA; BRATTON MR; BUNNELL BA; MARTIN Authors: VAN BOXTEL J; VAN DONGEN J; VONK LA;
EC STEVENS HP
58 - BONE-MARROW DERIVED MESENCHYMAL STEM/
25 - IN VITRO PBMC ASSAYS TO EVALUATE
STROMAL CELLS HAVE ENHANCED VASCULOGENIC
IMMUNOMODULATORY CELL PRODUCT POTENCY AND
POTENCY OVER ADIPOSE DERIVED MESENCHYMAL
PREDICT IN VIVO THERAPEUTIC RESPONDERS
STEM/STROMAL CELLS IN PERFUSED IN VITRO CULTURES
12:00 pm Presenter: Elaine Richards (USA) 12:00 pm
Presenter: Susanna Miettinen (Finland)
Affiliation: University of Florida
Affiliation: Tampere University
Authors: SAVA R; ZAMAN MO; HANDBERG EM; PEPINE CJ;
Authors: MYKULIAK A; YRJÄNÄINEN A; GEBRAAD A;
MARCH KL
VUORENPÄÄ H
59 - ISOLATION OF HUMAN ADIPOSE-DERIVED
STROMAL CELLS USING SUCTION-ASSISTED OR
26 - PERSONAL CELL THERAPY – THE BLACK SWAN OF ULTRASOUND-ASSISTED LIPOASPIRATION AND THEIR
REGENERATIVE MEDICINE CAN WE HEAL LYMPHEDEMA? THERAPEUTIC POTENTIAL IN CARTILAGE TISSUE
12:10 pm 12:10 pm
Presenter: Mark Berman (USA) ENGINEERING
Affiliation: University of Southern California Presenter: Jian Wang (China)
Affiliation: Suzhou Hospital of Anhui Medical University
Authors: CAI CH; ZHOU XU
60 - AN ALLOGENIC TISSUE-ENGINEERED PRODUCT
27 - CHARACTERIZATION AND POTENTIAL USE OF
FOR BONE REGENERATION ENABLING INDUCTION OF
NOVEL PLURIPOTENT ADIPOSE STEM CELLS (PASCS) IN
ENDOCHONDRAL OSSIFICATION AND INHIBITION OF
REGENERATIVE MEDICINE AND CELL THERAPY
12:20 pm 12:20 pm OSTEOCLASIA
Presenter: Gregorio Chazenbalk (USA)
Presenter: Hara Episkopou (Belgium)
Affiliation: UCLA
Affiliation: Novadip Biosciences SA
Authors: CASANUEVA DE ROSA P; GIMENO M
Authors: THEYS N; THIRION G
12:30 - 1:30 pm Lunch & Learn with Allergan Aesthetics an AbbVie company Los Olas Ballroom
Late Breaking Abstracts
1:30 - 2:00 pm Los Olas Ballroomr
Moderators: Bruce Bunnell, PhD & Jeffery M. Gimble, MD, PhD
Main Auditorium - Los Olas Ballroom Room 2 - Bonnet
Free Papers 4 - Characterization and Behavior Free Papers 9 - Radiation Injury
2:00 - 3:00 pm 2:00 - 3:00 pm
Moderators: Kacey Marra, PhD & Rosalyn Abbott, PhD Moderators: Julie Fradette, PhD & Pietro Gentile, MD
28 - EXADEX: TOWARDS A MICROPHYSIOLOGICAL
SYSTEM MODELING HUMAN ADIPOSE PROGENITOR 61 - ADIPOSE MESENCHYMAL STEM CELLS TREATMENT
CELL EXPANSION IN AN OBESE ADIPOSE TISSUE LIMITS CHRONIC RADIATION CYSTITIS
MICROENVIRONMENT Presenter: Clement Brossard (France)
2:00 pm Presenter: Christian Dani (France) 2:00 pm Affiliation: IRSN
Affiliation: iBV Authors: LEFRANC AC; DOS SANTOS M; DEMARQUAY C;
Authors: DANI V; BRUNI-FAVIER S; CARRIÈRE A; BUARD V; TARLET G; SQUIBAN C; LINARD C; MATHIEU N;
DEVINEAUX L; KÉOPHIPHATH M; CHIGNON-SICARD B; SIMON JM; MILLIAT F; CHAPEL A
CASTEILLA L; DOGLIO A; DANI C
Fort L au d erd al e, F lo r id a 1118th Annual IFATS Conference N ovem ber 18- 20, 2021
29 - IN SITU TRANSPLANTATION OF ADIPOSE-DERIVED 62 - ALLOGENEIC ADIPOSE DERIVED STEM CELLS
STEM CELLS VIA PHOTOACTIVATION IMPROVES GLUCOSE MITIGATE ACUTE RADIATION SYNDROME
METABOLISM IN OBESE MICE Presenter: Somaiah Chinnapaka (USA)
2:10 pm 2:10 pm
Presenter: Jianbo Wu (China) Affiliation: University of Pittsburgh
Affiliation: Southwest Medical University Authors: YANG S; SAMADI Y; EPPERLY M; HOU W;
Authors: ZHU LZ GREENBERGER J; EJAZ A; RUBIN P
63 - CLINICAL TRIAL EVALUATING THE EFFICACY OF
MESENCHYMAL STROMAL CELL INJECTIONS FOR THE
30 - REGULATION OF ADIPOGENIC POTENTIAL IN TREATMENT OF CHRONIC PELVIC COMPLICATIONS
DEDIFFERENTIATED LIPOSARCOMAS INDUCED BY RADIATION THERAPY
Presenter: Elizabeth Floyd (USA) Presenter: Alain Chapel (France)
2:20 pm 2:20 pm
Affiliation: Pennington Biomedical Research Center Affiliation: IRSN
Authors: DANG TN; TIONGCO RP; BROWN LM; TAYLOR JL; Authors: SEMONT A; LINARD C; MATHIEU N;
LYONS JM; LAU FH DEMARQUAY C; SQUIBAN C; VOSWINKEL J; ROUARD H;
LATAILLADE JJ; MARTINAUD C; BENDERITTER M; GORIN
NC; SIMON JM; MOTHY M
31 - MONOCYTE CHEMOATTRACTANT PROTEIN-1- 64 - ALLOGENEIC ADIPOSE TISSUE-DERIVED MATRIX
SUPPLEMENTED PLASMA ENHANCE ADIPOGENESIS MITIGATE RADIATION-INDUCED FIBROSIS (RIF)
THROUGH IL-33/DPP4 Presenter: Asim Ejaz (USA)
2:30 pm 2:30 pm
Presenter: Lee-Wei Chen (Taiwan) Affiliation: University of Pittsburgh
Affiliation: Kaohsiung Veterans General Hospital Authors: CHINNAPAKA S; KATHERINE Y; EPPERLY M;
Authors: LI M; CHEN P WEN U; GREENBERGER J; RUBIN P
65 - ADIPOSE-DERIVED REGENERATIVE CELLS AND
LIPOTRANSFER IN ALLEVIATING BREAST CANCER-
32 - CHARACTERIZATION OF ADIPOSE TISSUE AND
RELATED LYMPHEDEMA: AN OPEN-LABEL PHASE I TRIAL
ADIPOSE-TISSUE DERIVED STEM CELLS IN LIPEDEMA
WITH 4-YEARS OF FOLLOW-UP
2:40 pm Presenter: Sara Al-Ghadban (USA) 2:40 pm
Presenter: Mads Jørgensen (Denmark)
Affiliation: University of North Texas Health Science Center
Affiliation: Odense University Hospital
Authors: HERBST KL; BUNNELL BA
Authors: TOYSERKANI NM; JENSEN CH; ANDERSEN DC;
SHEIKH SP; SØRENSEN JA
33 - VITAMIN D AS A METABOLIC LIPOPROTECTANT: 66 - LONG TERM FOLLOW UP IN FEMALE LICHEN
PHARMACOLOGIC ENHANCEMENT OF FAT GRAFT SCLEROSUS FAT GRAFT
SURVIVAL Presenter: Massimiliano Brambilla (Italy)
2:50 pm Presenter: Phoebe Lee (USA) 2:50 pm Affiliation: IRCCS FONDAZIONE CA GRANDA OSPEDALE
Affiliation: University of Pittsburgh School of Medicine MAGGIORE
Authors: LODER SL; LEFTWICH PA; NERONE WN; SINGH- Authors: BOERO V; LIBUTTI G; RONCELLA E; IORIO M;
VARMA AS; MARRA KM; RUBIN PR; KOKAI LK CETERA G; CAIA C; MONTI E; VERCELLINI P
Main Auditorium - Los Olas Ballroom Room 2 - Bonnet
Free Papers 5 - Clinical Topics I Free Papers 10 - Matrix
3:00 - 4:00 pm 3:00 - 4:00 pm
Moderators: Ivona Percec, MD, PhD & Filip Stillaert, MD Moderators: Rosalyn Abbott, PhD & Aaron W. James, MD
67 - COMBINATION OF LYOPHILIZED ADIPOSE-DERIVED
34 - INJECTABLE TISSUE REPLACEMENT AND
STEM CELL CONCENTRATED CONDITIONED MEDIUM
REGENERATION (ITR2): OBJECTIVE ANALYSIS OF FACIAL
AND POLYSACCHARIDE HYDROGEL IN THE INHIBITION
VOLUME 19 MONTHS AFTER TREATMENT
3:00 pm 3:00 pm OF HYPERTROPHIC SCARRING
Presenter: Steven Cohen (USA)
Presenter: Xiasong Chen (China)
Affiliation: University of California San Diego
Affiliation: Union Hospital Of Fujian Medical University
Authors: WESSON J; TIRYAKI T
Authors: CHEN PH
12 For t Lauderdal e, Fl ori daN ove mb er 18- 20, 2 0 2 1 18th Annual IFATS Conference
68 - DIFFERENCES OF EMBEDDING ADIPOSE-DERIVED
35 - ENGINEERED FAT GRAFT ENHANCED WITH ADIPOSE-
STROMAL CELLS IN NATURAL AND SYNTHETIC
DERIVED STROMAL VASCULAR FRACTION CELLS FOR
SCAFFOLDS FOR DERMAL AND SUBCUTANEOUS
BREAST AUGMENTATION AND RECONSTRUCTION:
DELIVERY
3:10 pm CLINICAL, HISTOLOGICAL AND INSTRUMENTAL 3:10 pm
Presenter: Frederik Mamsen (Denmark)
EVALUATION
Affiliation: StemMedical
Presenter: Pietro Gentile (Italy)
Authors: MUNTHE-FOG L; KRING MK; DUSCHER D;
Affiliation: University of Rome Tor Vergata
TAUDORF M; KATZ AJ; KØLLE SF
69 - SKIN-DERIVED EXTRACELLULAR MATRIX
HYDROGELS LOADED WITH ADIPOSE TISSUE-DERIVED
36 - STUDY INTO THE EFFECT OF FAT GRAFTING ON SKIN STROMAL CELL- SECRETED FACTORS AS TREATMENT
CONTRACTION IN VITRO FOR ENHANCING WOUND REGENERATION AND
3:20 pm Presenter: Victoria Workman (United Kingdom) 3:20 pm VIABILITY OF SKIN FLAPS: A RAT MODEL
Affiliation: University of Sheffield Presenter: Viktor Sinkunas (Netherlands)
Authors: GIBLIN V; GREEN NH; MACNEIL S; HEARNDEN V Affiliation: University and Medical Center Groningen
Authors: VAN DONGEN JA; CAMARGO CP; VAN DER LEI
B; HARMSEN MC; VRIEND L
70 - THE EFFECT OF STROMAL VASCULAR FRACTION
37 - SKIN REJUVENATION USING CONCENTRATE
CELLS MIXED WITH DIFFERENT TYPES OF HYALURONIC
STROMAL VASCULAR TISSUE: AN INNOVATIVE TECHNIQUE
ACID FILLERS IN IMMUNODEFICIENT MICE
3:30 pm FOR DIFFICULT AREAS 3:30 pm
Presenter: Heetae Koo (SouthKorea)
Presenter: Sophie Menkes (Switzerland)
Affiliation: Un Seoul National University Hospital
Affiliation: Forever Institut
Authors: JIN US
38 - TISSUE STROMAL VASCULAR FRACTION TO PREVENT
DERMAL SCARRING: A PROSPECTIVE RANDOMIZED
71 - 3D PRINTED MICRONIZED FAT-LADEN COLLAGEN
MULTICENTER CLINICAL TRIAL
CONSTRUCTS FOR TREATMENT OF CHRONIC WOUNDS
Presenter: Joris van Dongen (Netherlands)
3:40 pm 3:40 pm Presenter: Nathan Katz (USA)
Affiliation: University Medical Center Groningen
Affiliation: Jointechlabs Inc
Authors: VAN BOXTEL J; UGUTEN M; BROUWER LA;
Authors: SCHMITT T.; KISHORE V.
VERMEULEN KM; MELENHORST WB; NIESSEN FB;
HARMSEN MC; STEVENS HP; VAN DER LEI B
72 - EXTRACELLULAR MATRIX-DERIVED HYDROGELS TO
AUGMENT DERMAL WOUND HEALING: A SYSTEMATIC
39 - PRECISE SUBDERMAL FAT GRAFT INJECTION
REVIEW
TECHNIQUE FOR SAFE GLUTEAL AUGMENTATION
3:50 pm 3:50 pm Presenter: Cristina Camargo (Netherlands)
Presenter: Ricardo Rodriguez (USA)
Affiliation: University and Medical Center Groningen
Affiliation: Cosmeticsurgnet
Authors: SINKUNJAS V; VRIEND L; VAN DER LEI B;
HARMSEN MC; VAN DONGEN JA
4:00 - 4:30 pm Coffee Break in Exhibit Hall Los Olas Foyer
Main Auditorium - Los Olas Ballroom Room 2 - Bonnet
Free Papers 11- Skin and Scars and Pain
Free Papers 6 - Adipose Tissue Processing
4:30 - 5:30 pm 4:30 - 5:30 pm Moderators: Alexandra Conde-Green, MD
Moderators: Kotaro Yoshimura, MD & Fred Kølle, MD, PhD
& Ivona Percec, MD, PhD
40 - TOWARDS STANDARDIZATION IN MECHANICALLY 73 - STROMAL VASCULAR FRACTION-ENRICHED FAT
ISOLATED STROMAL VASCULAR FRACTION RESEARCH – GRAFTING AS TREATMENT OF ADHERENT SCARS: STUDY
PRELIMINARY RESULTS OF A METHODOLOGICAL ANALYSIS DESIGN OF A PROSPECTIVE COHORT STUDY
Presenter: Ann-Sophie Madeleyn (Belgium) Presenter: Linda Vriend (Netherlands)
4:30 pm 4:30 pm
Affiliation: Ghent University Affiliation: University and Medical Center Groningen
Authors: DÍAZ JM; LOBATO RC; BUELVAS BUSTILLO AM; Authors: VAN DONGEN JA; PIJPE A; NIEUWENHUIS MK;
SINGH K; BLONDEEL PN; TONNARD PL; VERPAELE A; JONGEN SJ; HARMSEN MC; VAN ZUIJLEN PM; VAN DER
GHIASLOO M LEI B
Fort L au d erd al e, F lo r id a 1318th Annual IFATS Conference N ovem ber 18- 20, 2021
41- IMPROVING THE TECHNIQUE OF NON-ENZYMATIC
74 - DIRECT COMPARISON OF BIOLOGICAL-BASED
METHOD FOR OBTAINING THE STROMAL-VASCULAR
THERAPIES ON HUMAN FOLLICLE DERMAL PAPILLA
FRACTION
CELL FUNCTION
4:40 pm Presenter: Natalia Khramtsova (Russia) 4:40 pm
Presenter: John Cole (USA)
Affiliation: EA Vagner Perm State Medical University
Affiliation: PHTC
Authors: PLAKSIN SA; SOTSKOV AY; PONOMAREV DN;
Authors: COLE MA
GULYAEVA NI
75 - AUTOLOGOUS FAT GRAFTING AS TREATMENT OF
42 - TO LYSE, OR NOT TO LYSE? POSTHERPEUTIC NEURALGIA - RESULTS OF A DOUBLE-
Presenter: Gabriela Aguilo-Seara (USA) BLINDED PLACEBO-CONTROLLED RCT
4:50 pm 4:50 pm
Affiliation: Wake Forest School of Medicine Presenter: Martin Sollie (Denmark)
Authors: SHANG H; NORTHRUP S; LLULL R; KATZ AJ Affiliation: Odense University Hospital
Authors: THOMSEN JB; SØRENSEN JA
43 - CELL VIABILITY IS NOT COMPROMISED IN
MECHANICALLY SHEARED CELL-DENSE STROMAL CELL 76 - REVIEW OF ADIPOSE GRAFTING AND PLATELET
AGGREGATES RICH PLASMA FOR VOLUMIZATION AND SCAR RELEASE
5:00 pm Presenter: Ramon Llull (USA) 5:00 pm Presenter: Sherry Collawn (USA)
Affiliation: Wake Forest School of Medicine Affiliation: UAB
Authors: SESE B; MONSERRAT J; SHANG H; SANFILLIPO Authors: JAFFE TJ; TEKUMALLA ST
WA; KATZ AJ; AGUILO SEARA G
44 - LONG TERM CLINICAL RESULTS OF MECHANICAL
77 - TRANSDERMAL NANOFAT DELIVERY ACCELERATES
STROMAL-CELL TRANSFER (MEST) AND ADJUSTABLE
SKIN RESURFACING RECOVERY FOLLOWING CO2 LASER
REGENERATIVE ADIPOSE TRANSFER (ARAT) BY USING
AND MINIMALLY INVASIVE PERCUTANEOUS COLLAGEN
5:10 pm ULTRA-SHARP BLADES 5:10 pm
INDUCTION TREATMENT
Presenter: Eray Copcu (Turkey)
Presenter: Brannon Claytor (USA)
Affiliation: MEST
Affiliation: Claytor/Noone Plastic Surgery
Authors: OZTAN S
45 - DEVELOPMENT AND VALIDATION OF A FULLY GMP-
COMPLIANT PROCESS FOR MANUFACTURING STROMAL
VASCULAR FRACTION: A COST-EFFECTIVE ALTERNATIVE TO
78 - SYNGENEIC FAT GRAFTING ATTENUATES
AUTOMATED METHODS
HYPERSENSITIVITY IN A LEWIS RAT NERVE CRUSH
Presenter: Pauline Francois (France)
INJURY MODEL
5:20 pm Affiliation: C2VN Aix marseille univ INSERM 1263 INRA 5:20 pm
Presenter: Benjamin Scott (USA)
1260
Affiliation: Massachusetts General Hospital
Authors: MAGALON J; GIRAUDO L; VERAN J;
Authors: MCCORMACK MC; BEJAR MK; AUSTEN WG
BERTRAND B; DUMOULIN C; ABOUDOU H; GRIMAUD F;
VOGTENSPERGER M; VELIER M; ARNAUD L; LYONNET L;
SIMONCINI S; GUILLET B; DIGNAT-GEORGE F; SABATIER F
Guest Speaker - Camilo Ricordi, MD
Micro-fragmented Tissue in Regenerative Surgery: A Transformational
5:30 - 6:30 pm Cost-effective Opportunity for the Future Los Olas Ballroomr
of Healthcare
Moderator: Stuart Williams, PhD
6:30 pm Meeting Adjourns for the day
14 For t Lauderdal e, Fl ori daN ove mb er 18- 20, 2 0 2 1 18th Annual IFATS Conference
• SATURDAY - NOVEMBER 20
7:30 - 8:30 am Continental Breakfast in Exhibit Hall Los Olas Foyer
8:00 - 9:00 am IFATS Members Meeting Los Olas Ballroom
Guest Speaker - Arnold Caplan, PhD
9:00 - 10:00 am MSCs: Bigger and Better than Ever Los Olas Ballroom
Moderator: Ricardo Rodriguez, MD
10:00 - 10:30 am Coffee Break in Exhibit Hall Los Olas Foyer
Plenary Session 3 - Regulatory Affairs
10:30 - 11:30 am Moderators: Adam Katz, MD & Keith March, MD Los Olas Ballroom
Panelists: Mark Berman,MD, Bernard Siegel, MD, Keith March, MD, Doug Oliver, MSW
11:30 - 11:45 am Closing Remarks - Torsten Blunk, PhD Los Olas Ballroom
11:45 am Meeting Adjourns
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Fort L au d erd al e, F lo r id a 1518th Annual IFATS Conference N ovem ber 18- 20, 2021
ABSTRACTS
ABSTRACT 1 ABSTRACT 2
MESENCURE—AN ENHANCED ALLOGENEIC ADIPOSE- DEVELOPMENT OF A 3D BIOPRINTED BREAST CANCER-
DERIVED CELL THERAPY DEVELOPED FOR TREATING ACUTE STROMA CO-CULTURE MODEL UTILIZING ADIPOSE-DERIVED
RESPIRATORY DISTRESS IN COVID-19: FROM BENCHTOP TO STROMAL CELL SPHEROIDS
BEDSIDE
Presenter: Hannes Horder (Germany)
Presenter: Tomer Bronshtein (Israel) Affiliation: University of Wuerzburg
Affiliation: Bonus BioGroup Ltd. Authors: GUAZA LASHERAS M; GRUMMEL N; NADERNEZHAD A;
Authors: BEN DAVID D; NOVAK A; KIVITY V; HAMOUD S; HERBIG J; ERGUN S; TESSMAR J, GROLL J; FABRY B; BAUER-KREISEL
HAYEK T; MERETZKI S P, BLUNK T
Mesenchymal stromal cells (MSC) have attracted much attention Introduction: In breast cancer, stromal cells associated with the
for treating ARDS in Covid-19. These efforts are, nonetheless, tumor microenvironment, such as adipose-derived stromal cells (ASCs)
overshadowed by earlier studies that showed marginal efficacy and adipocytes, contribute to various stages of tumor development,
attributable to loss of MSC potency. Also, concerns were raised progression, and metastasis. To investigate the complex interplay
regarding the hemocompatibility of MSCs in coagulopathic Covid-19 between tumor and stromal cells in vitro in a more physiological
patients. Relying on years of experience and manufacturing capacity context, we developed a 3D breast cancer-stroma model utilizing
of clinical-grade MSCs, Bonus BioGroup developed MesenCure—an bioprinted human ASC spheroids and MDA-MB-231 breast cancer cells.
enhanced allogeneic adipose-derived MSC product for IV injection
professionalized to treat ARDS in Covid-19. Methods: ASC spheroids were generated on a large-scale and
dispersed in a thiol-modified hyaluronic acid bioink for the use in
MesenCure is based on technologies for the efficient and standardized an extrusion-based bioprinting setup. Viability and differentiation
isolation and cultivation of adipose MSCs yielding 600k p0 MSCs per capability of the printed spheroids were assessed. Adipogenically
ml fat and up to 45k doses per donor cell bank under 20 PDLs. In differentiated and undifferentiated spheroids were co-cultured with
addition, the cells are formulated for cold storage, avoiding potency MDA-MB-231 breast cancer cells in printed 3D constructs. Cancer-
loss associated with cryopreservation while providing sufficient shelf- induced alterations of the lipid content and ECM composition of the
life for global supply. Importantly, MesenCure cells are professionalized spheroids were analyzed after 9 days of co-culture.
to treat ARDS by exposure to a combination of biological and physical
priming conditions that enhance their potency and safety. Results: Bioink composition and the printing process were optimized
with regard to homogeneous spheroid distribution and cell viability.
Compared to unprimed MSCs, MesenCure cells express higher levels The printing process had no negative impact on the differentiation
of immunomodulatory and regenerative genes, including EDIL3, IL6R, behavior of spheroids, as shown regarding triglyceride accumulation
and FGF7. In vitro, MesenCure inhibited the proliferation of activated and adipogenic marker gene expression (PPARγ, C/EBPα, FABP4).
PBMCs twice more effectively than unprimed MSCs. MesenCure, but Differentiated spheroids developed into adipose microtissues,
not unprimed MSCs, alleviated edema in an acute lung injury model exhibiting a native adipose tissue-like ECM composition, with highly
by 60% and reduced the leukocytes’ counts in the lung fluids by 40% elevated levels of laminin and collagen IV, enhanced collagen I and VI,
while reversing the pneumonia pathological presentation. MesenCure and a marked reduction of fibronectin. In the printed co-culture model,
cells are also more hemocompatible with 50% less Tissue Factor at the differentiated spheroids revealed a significant reduction of lipid content
mRNA, protein, and activity levels and >2-fold higher level of Tissue and ECM remodeling with increased collagen I, VI and fibronectin,
Factor Pathway Inhibitor compared to unprimed MSCs. as compared to the monoculture control. Undifferentiated spheroids
also showed ECM remodeling, with increased levels of tenascin c and
MesenCure is tested in a Phase II study in severe Covid-19 patients collagen I.
hospitalized at the Rambam Health Care Campus (Haifa, Israel).
Results from a Phase I/II study on ten such patients with high-risk Conclusions: A 3D-bioprinted breast cancer model was developed
comorbidities (90%) demonstrated significant improvements in all as proof-of-concept that recapitulates characteristic cancer cell-
objective and subjective health parameters following MesenCure induced alterations in stromal cells known from in vivo studies, such
treatment with no IMP-related AEs. Patients were discharged within 1 as reduction of lipid content in adipocytes and ECM remodeling. The
day following treatment, requiring no respiratory support and pointing biofabricated 3D model may facilitate to further decipher the complex
to MesenCure’s potential for treating ARDS and other conditions. tumor-stroma crosstalk in breast cancer in a defined, native-like
environment.
16 For t Lauderdal e, Fl ori daN ove mb er 18- 20, 2 0 2 1 18th Annual IFATS Conference
ABSTRACT 3 ABSTRACT 4
VASCULARIZED ADIPOSE TISSUE GRAFT USING A ADIPOSE STEM CELL-BASED THERAPIES FOR FIBROSIS AND
DECELLULARIZED LUNG FOR SOFT TISSUE RECONSTRUCTION SCARRING OF THE VULVA
Presenter: Rosalyn Abbott (USA) Presenter: Aurora Almadori (Italy)
Affiliation: Carnegie Mellon University Affiliation: UCL - Royal Free Hospital
Authors: DEBARI MK; NG WH; KOKAI LE; MARRA KG; RUBIN JP; Authors: BOYLE D; HANSEN E; REID W; MACLEAN A; BUTLER PE
REN X
Introduction: Minimally invasive regenerative therapies including
Introduction: Innovation is needed to engineer critically sized adipose autologous fat transfer, adipose-derived stem cells (ASCs) and platelet-
tissue implants that can fill large tissue defects, rapidly integrate rich plasma (PRP) have been proposed as new option to treat women
into the host vascular system and maintain volume. This will result in with fibrosis and scarring in the vulvar and perineal area. The aim of
minimal donor site morbidity, can be implemented in lean patients, the study was to evaluate the outcome in a mixed cohort of women
and eliminates the need for immunosuppressive therapy. By using a presenting fibrosis and scarring of the vulva due to lichen sclerosus,
decellularized lung matrix (DLM), perfusable, pre-vascularized, large- post-partum episiotomy/lacerations, and female genital mutilation.
volume adipose grafts can be developed using patient-derived cells.
Materials & Methods: A series of 50 patients was included and
Materials & Methods: Rat lungs were isolated and decellularized. prospectively assessed. The outcome evaluation included: clinical signs
Mature adipocytes and adipose derived stem cells (ADSCs) were of disease assessed with the Vulvar Architecture Severity Scale (VASS),
isolated from primary human adipose tissue obtained from liposuction/ symptoms (Visual Analogue Scale), sexual function (Female Sexual
panniculectomies. Human umbilical vein endothelial cells were seeded Function Index), sexual distress (Female Sexual Distress Scale), anxiety
into the DLM through the vasculature two hours before the cell and depression (Hospital Anxiety and Depression Scale), and romantic
solution was injected into the alveoli through the trachea. The lung was relationship (Relationship Assessment Scale).
perfused with adipocyte maintenance media (DMEM, 10% FBS, and
1% Pen/Strep) through the vasculature for seven days. The lungs were Results: The clinical score showed an overall improvement in the
then fixed and stained (Adipored and CellTracker green dye). Separate treated areas, particularly in labia minora, clitoral area and posterior
lungs were seeded with ADSCs and cultured statically in differentiation fourchette (p18th Annual IFATS Conference N ovem ber 18- 20, 2021 ABSTRACT 5 ABSTRACT 5 Images: CHANGES IN EXPANSION PRESSURES IN EXPANDER-BASED POST-MASTECTOMY DEFECT RECONSTRUCTION: THE EFFECT OF FAT GRAFTING IN NORMAL AND RADIATED TISSUES Presenter: Donald Browne (USA) Affiliation: Wake Forest Baptist Health Authors: MONSERRAT J; MATAS A; SESE B; LLULL R Introduction: The impact of fat grafting on the viscoelasticity of irradiated tissues is poorly defined. We investigate the effect of subcutaneous fat grafting on post-mastectomy tissue expansion in patients undergoing delayed breast reconstruction. We hypothesize fat grafting changed the trophic and viscoelastic properties of the breast soft tissue envelope. Methods: Post-mastectomy defects delayed more than two years and reconstructed with sub-pectoral tissue expanders were prospectively studied. Control (no irradiation, no fat grafting, n=7), fat grafted control (no irradiation, fat grafting n=8), and radiated mastectomy (irradiation, fat grafting, n=9) groups were included (Figure 1). Hydrostatic pressure of the tissue was measured before and immediately after expansion, and again post expansion day 1 and prior to the following expansion session (Figure 2). Pressure changes calculated as “post expansion-relaxation interval”: difference between maximal pressure at each expansion and the minimal pressure prior to each expansion session. Results: Hydrostatic pressure plots reflect the soft tissue envelope’s ability to accommodate sequential expansion. Fat grafted breasts demonstrated a statistically significant increased “post expansion- relaxation interval” versus the non-grafted control group (P< 0.0001, Figure 3). Viscoelastic changes impact the overall expansion time: the fat grafted group achieved total expansion two weeks earlier than the nongrafted control group (P< 0.05). The fat grafted, radiated group completed expansion in similar time interval as non-grafted control group. Complications were not significantly different between the irradiated, grafted breasts and control breasts. Conclusions: Observed viscoelastic changes impact the overall expansion time. Fat grafting in non-radiated mastectomy defects allows for shorter expansion period. Fat grating in radiated mastectomy defects appears to allow safe expansion, and expansion durations equivalent to non-radiated, non-fat grafted control defects. 18 For t Lauderdal e, Fl ori da
N ove mb er 18- 20, 2 0 2 1 18th Annual IFATS Conference
ABSTRACT 6 ABSTRACT 7
STROMAL VASCULAR FRACTION MITIGATES RADIATION- IMMUNOMODULATORY AND REGENERATIVE EFFECTS OF THE
INDUCED GASTRO-INTESTINAL SYNDROME FULL AND FRACTIONED ADIPOSE TISSUE DERIVED STEM CELLS
SECRETOME IN SPINAL CORD INJURY
Presenter: Lydia Bensemmane (France)
Affiliation: IRSN Presenter: Antonio Salgado (Portugal)
Authors: SQUIBAN C; DEMARQUAY C; MATHIEU N; Affiliation: University of Minho
BENDERITTER M; MILLIAT F; LINARD C Authors: CIBRÃO J.; PINHOE A.; LIMA R; GOMES E; SERRA S;
LENTILHAS-GRAÇA J; RIBEIRO C; LANCEROS-MENDEZ S;
Introduction: The intestine is particularly sensitive to moderate-high TEIXEIRA F; MONTEIRO S; SILVA N
radiation dose and the development of gastrointestinal syndrome
(GIS) leads to the rapid loss of intestinal mucosal integrity, resulting in Spinal cord Injury (SCI) is a dramatic pathology, with a high number
bacterial infiltration, sepsis that comprise patient survival, the death of new cases emerging every year. The injury itself triggers several
occurs between 10 and 15 days post-exposure. There is an urgent biological events such as the activation of apoptotic pathways, the
need for effective and rapid therapeutic countermeasures. The stromal release of inflammatory cytokines and also the formation of a glial scar
vascular fraction (SVF) derived from adipose tissue is an easily, rapidly that primarily contains further damage, but also releases biomolecules
accessible source of cells with angiogenic, anti-inflammatory and that inhibit axons outgrowth, causing motor and sensory functional
regenerative properties. We studied the therapeutic impact of SVF and loss below the level of the spinal cord which translates in poor living
its action on the intestinal stem cell compartment. conditions to the patients.
Methods: Mice exposed to the abdominal radiation (18 Gy) received In this study, we intended to dissect the role of the secretome of
a single intravenous injection of stromal vascular fraction (SVF) adipose tissue derived stem cells (ASCs), as well as its individual
(2.5*106 cells), obtained by enzymatic digestion of inguinal fat tissue, vesicular and proteic individual fractions, in in vitro and in vivo models
on the day of irradiation. Mortality was evaluated as well as intestinal of axonal growth, inflammation and spinal cord injury, respectively.
regeneration by histological analyses and absorption function. In vitro experiments revealed that the unfractionated secretome had
a significant effect growth, when compared o its protein or vesicular
Results: The SVF treatment limited the weight loss of the mice and fractions, on axonal growth and neuroinflammatory profile of microglial
inhibited the intestinal permeability and mortality after abdominal cells. Following on this data we then evaluated the impact of ASCs
irradiation. Histological analyses of intestine showed that SVF secretome on the histological and functional recovery of a severe
treatment stimulated the regeneration of the epithelium by promoting compression-based SCI model in mice. Results of these experiments
numerous enlarged hyperproliferative zones. SVF restored CD24+/ revealed that ASCs secretome induced a significant improvement
lysozyme- and Paneth cell populations in the intestinal stem (p18th Annual IFATS Conference N ovem ber 18- 20, 2021
ABSTRACT 8 ABSTRACT 9
WHARTON’S JELLY MSC-DERIVED SMALL EXTRACELLULAR MESENCHYMAL STEM CELL-DERIVED EXTRACELLULAR
VESICLES: A NATURALLY NANOTHERAPEUTIC AGENT VESICLES IN AUTOLOGOUS FAT GRAFTING – LESSONS FROM A
AMELIORATES OSTEOARTHRITIS BY CARRYING MIRNA CARGO METHODOLOGICAL ANALYSIS
Presenter: Penghong Chen (China) Presenter: Mohammad Ghiasloo (Belgium)
Affiliation: Fujian Medical University Union Hospital Affiliation: Ghent University
Authors: CHEN XS Authors: DE WILDE L; SINGH K; TONNARD PL; VERPAELE A;
DE WEVER O; HENDRIX A; BLONDEEL PN
Background: As a cell-free nanotherapeutic agent, extracellular
vesicles can effectively surmount many obstacles derived from Introduction: The mesenchymal stem cell-derived extracellular
mesenchymal stem cell-based therapy or synthetic nanoparticles in vesicles (MSC-EVs) are a promising cell-free modality in autologous
regenerative medicine. Here, our study aimed to investigate the therapeutic fat grafting (AFG). However, the study of EVs mandates a rigorous
effects and the mechanism of Wharton’s jelly mesenchymal stem cell- methodology and transparent reporting –as established by the
derived extracellular vesicle (WJMSC-sEVs) in osteoarthritis (OA). MISEV2018 guidelines– thereby facilitating reproducibility. We
methodologically evaluated studies investigating MSC-EV-enriched AFG
in preclinical studies.
Methods: WJMSC-sEVs were isolated by size-exclusion
chromatography. In vitro, IL-1?-induced OA chondrocytes were Methods: A systematic review was performed according to the
established to evaluate cell proliferation and migration by CCK-8 PRISMA guidelines. Embase, PubMed and CENTRAL databases were
assay and transwell assay, respectively. Meanwhile, OA-related and used. Included studies were submitted to EV-TRACK (http://evtrack.org)
macrophage-related genes expression was quantified using qPCR. In –a crowdsourcing knowledgebase– thereby producing the EV-METRIC
vivo, a rat OA model were induced by transecting anterior cruciate of the experiments within the individual studies. The EV-METRIC
ligament. This was followed by intra-articular injection of either is expressed as a percentage of fulfilled components from a list of
WJMSCs or their sEVs. Cartilage destruction was evaluated with nine –evaluating the EV isolation method, as well as biophysical and
micro-CT, histological and immunohistochemistry analyses. Next, small biochemical analysis of separated EVS– with each component deemed
miRNA-seq was used to explore the protective mechanism of WJMSC- as indispensable for unambiguous interpretation and independent
replication of EV experiments.
sEVs on chondrocytes.
Results: Of 2474 studies, 61 underwent full text screening. Finally,
Results: WJMSC-sEVs were approximately 50–150 nm in diameter seven studies were included, consisting of 12 experiments. The EV-
and expressed CD9, CD63, and TSG101 but did not express calnexin. METRIC was 15-38% (Figure 1). Six different EV isolation methods
sEVs successfully fused into chondrocyte, and attenuated IL-1?- were observed, mainly based on a combination of (differential)(ultra)
induced chondrocyte proliferation and migration inhibition. WJMSC- centrifugation and filtration. No density gradient centrifugation
sEVs increased chondrogenic genes Col2A1, decreased MMP13 was used, nor were separated EVs analyzed for their density.
and ADAMTS5. Furthermore, WJMSC-sEVs promoted macrophage Biophysical analysis could only be reproduced in 5/12 (42%) and
polarization toward the M2 phenotype. In the animal study, both 2/12 (17%) experiments, respectively. Western blotting was used in
of WJMSCs and WJMSC-sEVs treatment significantly upregulated 9 experiments to analyze the EV-enriched –positive marker– and non
Collagen II and downregulated MMP13 and ADAMTS5 protein. EV-enriched proteins –negative marker– in the EV mixtures. None of
the experiments specified the EV lysate preparation method. Positive
Moreover, WJMSC-sEVs treatment promote subchondral bone
markers were used in 9/12 (75%) experiments, whereas negative
reconstruction similar to WJMSCs. Of note, we found that many potent markers were controlled in only 4/12 (33%) experiments. Finally, only
miRNAs abundant in WJMSC-sEVs has been confirmed to can promote 3/10 (30%) provided sufficient data on the used antibodies.
cartilage regeneration through activating PI3K/Akt, NOTCH and Hippo
pathway. Conclusion: MSC-EV-enrichment appears to be a novel approach in
improving AFG survival. However, a lack of transparency in reporting
Conclusion: WJMSC-sEVs possess great biocompatibility has limited the value of previous reports. Therefore, early adherence to
and effectively protect against cartilage erosion. The excellent the MISEV2018 guidelines and EV-TRACK knowledgebase should be
regeneration capacity of WJMSC-sEVs was attributed to EV-containing encouraged, thus allowing knowledge gaps to be identified at an early
miRNAs. Therefore, compared to their parent cells and complex stage.
EV-enriched proteins
synthetic nanoparticles, natural WJMSC-sEVs may be a preferred ABSTRACT 9 Image:
nanotherapeutic paradigm and will open clinically meaningful lysate preparation non EV-enriched proteins
perspectives for OA treatment.
qualitative and quantitative particle
antibody specifics
analysis
UC specifics electron microscopy images
EV density density gradient
Figure 1 Spider chart representing the percentage of included studies that adhere to each of the respective EV-METRIC parameters (http://evtrack.org/)
20 For t Lauderdal e, Fl ori daN ove mb er 18- 20, 2 0 2 1 18th Annual IFATS Conference
ABSTRACT 10 ABSTRACT 10 Images:
INVESTIGATING THE EFFECT OF FACTORS SECRETED FROM
ADIPOSE TISSUE ON MYOFIBROBLAST DIFFERENTIATION
Presenter: Samuel Higginbotham (United Kingdom)
Affiliation: The University of Sheffield
Authors: WORKMAN VL; GREEN NH; GIBLIN V; LAMBERT DW;
HEARNDEN V
Aberrant activation of fibroblasts to myofibroblasts is a key event in the
formation of hypertrophic scars. Dysregulated differentiation leads to
excessive production of extracellular matrix (ECM) components, such
as collagen, resulting in thick, inflexible, painful scars for which there is
no current effective treatment (1). Autologous fat grafting is an exciting
new surgical option that has been shown to regenerate and improve
the appearance and function of scar tissue (2). Despite this reported
effect, we still have a limited understanding of how adipose tissue
exerts these effects at a bio-molecular level and there is a gap between
our scientific understanding and clinical observations. The aim of this Aberrant activation of fibroblasts to myofibroblasts is a key event in the formation of hypertrophic
scars. Dysregulated differentiation leads to excessive production of extracellular matrix (ECM)
study was to determine how adipose tissues and factors secreted components, such as collagen, resulting in thick, inflexible, painful scars for which there is no current
from them affected fibroblast to myofibroblast differentiation and scar effective treatment (1). Autologous fat grafting is an exciting new surgical option that has been
shown to regenerate and improve the appearance and function of scar tissue (2). Despite this
behaviour in vitro. reported effect, we still have a limited understanding of how adipose tissue exerts these effects at a
bio-molecular level and there is a gap between our scientific understanding and clinical
Adipose tissue was processed into clinically relevant formulations and observations. The aim of this study was to determine how adipose tissues and factors secreted from
them affected fibroblast to myofibroblast differentiation and scar behaviour in vitro.
used to condition tissue culture medium. This was added to human
Adipose tissue was processed into clinically relevant formulations and used to condition tissue
dermal fibroblasts in combination with transforming growth factor culture medium. This was added to human dermal fibroblasts in combination with transforming
beta-1 (TGFβ-1, which stimulates myofibroblast differentiation). growth factor beta-1 (TGFβ-1, which stimulates myofibroblast differentiation). Following this,
markers of a myofibroblast phenotype were measured to assess the effect adipose tissue factors had
Following this, markers of a myofibroblast phenotype were measured to on differentiation.
assess the effect adipose tissue factors had on differentiation.
ECM components (collagen and fibronectin) and the myofibroblast marker α-SMA were significantly
reduced in treated cells demonstrating that factors secreted from adipose tissue can inhibit the
ECM components (collagen and fibronectin) and the myofibroblast differentiation of fibroblasts into myofibroblasts. Further analysis to elucidate the mechanisms
behind this anti-fibrotic effect has been performed to identify potential target molecules.
marker α-SMA were significantly reduced in treated cells demonstrating
Our data shows that adipose tissue can inhibit canonical TGFβ-1 signaling in dermal fibroblasts.
that factors secreted from adipose tissue can inhibit the differentiation Increasing our understanding of the mechanisms responsible for scar improvement presents new
of fibroblasts into myofibroblasts. Further analysis to elucidate the opportunities to design and optimise future fat-based regenerative therapies to improve patient
outcomes.
mechanisms behind this anti-fibrotic effect has been performed to
identify potential target molecules. 1.Darby I, Laverdet B, Bonté F, Desmoulière A. Fibroblasts and myofibroblasts in wound healing.
Clinical, Cosmetic and Investigational Dermatology 4, 7, 2014;
2.Klinger M, Marazzi M, Vigo D, Torre M. Fat injection for cases of severe burn outcomes: a new
Our data shows that adipose tissue can inhibit canonical TGFβ-1 perspective of scar remodeling and reduction. Aesthetic Plastic Surgery 119, 5, 2008;
signaling in dermal fibroblasts. Increasing our understanding of
the mechanisms responsible for scar improvement presents new
opportunities to design and optimise future fat-based regenerative
therapies to improve patient outcomes.
1.Darby I, Laverdet B, Bonté F, Desmoulière A. Fibroblasts and
myofibroblasts in wound healing. Clinical, Cosmetic and Investigational
Dermatology 4, 7, 2014;
2.Klinger M, Marazzi M, Vigo D, Torre M. Fat injection for cases of
severe burn outcomes: a new perspective of scar remodeling and
reduction. Aesthetic Plastic Surgery 119, 5, 2008;
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