Service Facilities for Research in Biotechnology - Volume 21, 2022 - Iowa State University Office of Biotechnology
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Office of Biotechnology
Service Facilities
Iowa State University
1210 Molecular Biology Building
2437 Pammel Drive
Ames, IA 50011-1079
for Research in Biotechnology
tel: 515-294-9818
e-mail: biotech@iastate.edu
www.biotech.iastate.edu
Volume 21, 20222022
Iowa State University
Service Facilities for Research in Biotechnology
Developed by
The Office of Biotechnology and The Office of the Vice President for Research
This publication serves as a directory of the service facilities for biotechnology-related research at Iowa State University.
These facilities are available to faculty at Iowa State University and individuals in other universities, government and in-
dustry who are interested in utilizing the facilities’ services for their research projects.
For more information, please contact: Office of Biotechnology, 1210 Molecular Biology Building, 2437 Pammel Drive,
Ames, IA 50011-1079. Email: biotech@iastate.edu. Voice: (515) 294-9818. Online: www.biotech.iastate.edu
Table of Contents
Animal Gene Transfer Facility............................................................. 1
BioCentury Research Farm.................................................................. 1
Biomolecular Nuclear Magnetic Resonance Facility.................. 2
Chemical Instrumentation Facility................................................... 3
Comparative Pathology Core Service............................................. 5
Center for Crops Utilization Research............................................. 6
Cryo-Electron Microscopy Facility.................................................... 7
DNA Facility.............................................................................................. 8
Doubled Haploid Facility..................................................................... 10
Fermentation Facility............................................................................ 11
Flow Cytometry Facility........................................................................ 12
Genome Informatics Facility............................................................... 14
Genomic Technologies Facility.......................................................... 15
Grain Quality Laboratory..................................................................... 16
Roy J. Carver High Resolution Microscopy Facility..................... 17
Hybridoma Services.............................................................................. 20
Macromolecular X-ray Crystallography Facility........................... 21
Materials Analysis and Research Laboratory................................ 22
Materials Preparation Center............................................................. 24
W. M. Keck Metabolomics Research Laboratory......................... 24
W. M. Keck Microfabrication Facility................................................ 25
Nutritional and Wellness Research Center
and Sensory Evaluation Unit...................................................... 26
Olfactory and Air Quality Laboratories........................................... 27
Protein Facility......................................................................................... 28
Iowa State University is an Equal Opportunity/Affirmative Action employer. All qualified applicants will receive con-
sideration for employment without regard to race, color, age, religion, sex, sexual orientation, gender identity, genetic
information, national origin, marital status, disability, or protected Veteran status and will not be discriminated against.
Inquiries can be directed to the Office of Equal Opportunity, 3410 Beardshear Hall, 515 Morrill Road, 515 294-7612,
email eooffice@iastate.edu.Animal Gene Transfer Facility
Location:
1270 Kildee Hall,
806 Stange Road
T he facility provides a variety of services to individuals who wish to utilize transgenic
animals as part of their research programs. The facility maintains equipment necessary for
the creation of transgenic animals via microinjection or somatic cell nuclear transfer and is
Ames, IA 50011-1178 available to life science researchers for technical service and for training of a limited number of
individuals. All work to be performed in this facility must be scheduled with the professor-in-
charge, as the facility is not staffed full-time.
Hours:
By appointment, Equipment housed in the facility includes two microinjection workstations, micropipette
8 a.m. to 5 p.m. weekdays pullers, a microforge, stereo-zoom microscope and CO2 incubator. Additional supporting
equipment located in nearby laboratories to which access may be granted includes a heated
Contact: microcentrifuge, tri-gas incubators, stereo-zoom microscopes, inverted microscope, biosafety
Jason Ross cabinet, osmometer, pH meter, embryo freezing machine, cell fusion machine, gel electropho-
jwross@iastate.edu resis equipment and UV transilluminator.
515-294-8647
515-294-4471 (fax) Service and user fees are determined on a case-by-case basis and are dependent upon
experimental factors.
BioCentury Research Farm
Location:
1327 U Avenue,
Boone, IA 50036
I owa State University’s BioCentury Research Farm (BCRF) is the first-in-the-nation
integrated research and demonstration facility dedicated to biomass production and
processing. It is located seven miles west of the Iowa State University campus on U.S.
Highway 30. The BCRF offers opportunities for large pilot scale and pre-commercial scale
research in biomass feedstock production, harvest, transport, storage, preparation, processing
Hours: and laboratory testing. Field plots for crop production trials, field equipment modification and
8 a.m. to 5 p.m. weekdays
testing facilities, biomass storage facilities and biomass conversion facilities are available for
Other hours by arrangement
internal and external users.
Contact: The biomass processing facility has three 2,700-square-foot pilot plant processing trains which
Matt Darr, Director house biochemical and thermochemical processing systems, four laboratories totaling 2,100
darr@iastate.edu square feet, and over 1,400 square feet of office space. The BCRF also has 4,200 square feet of
515-294-8545 dry biomass feedstock storage and 420 square feet of cold storage.
Andrew Suby, Manager Services available at the BCRF include biomass feedstock production; biomass preparation
asuby@iastate.edu (sizing, drying and chemical pretreat); fermentation production of fuels, industrial chemi-
515-296-6039 cals, and other products (500- and 1,000-liter capacities); thermochemical processing via fast
pyrolysis at a rate of 22 kilograms/hour; solvent liquefaction at a rate of 1 kilogram/hour; and
Rob Hartmann, gasification at a rate of 35 kilograms/hour. The BCRF also has a 720-square-foot algal produc-
Facility Coordinator tion facility capable of producing algal biomass of 1.3-4.5 kilograms/week and a biopolymer
rchartma@iastate.edu processing plant which can process 500 kg/day.
515-296-6302
More information is available online at http://www.biocenturyresearchfarm.iastate.edu.
Fax: 515-432-2701
Web:
http://www.biocentury
research farm.iastate.edu
2022 1B iomolecular Nuclear Magnetic Resonance Facility
Location:
0208 Molecular Biology
T he Biomolecular Nuclear Magnetic Resonance (NMR) Facility is supported by the
Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology. The
facility currently operates Bruker Avance II 700 and Avance III 800 spectrometers, each
Building, 2437 Pammel Drive, capable of performing a broad range of modern multi-nuclear, multi-dimensional NMR
Ames, IA 50011-1079 experiments on biomolecules. The 700 is equipped with a 5 mm H/C/N cryoprobe, a 5 mm
H/C/BB conventional probe and a 3.2 mm H/BBX/BBY-MAS solids probe. The 800 is equipped
Hours: with a 5 mm H/C/N cryoprobe, a 5 mm H/C/BB conventional probe and 3.2 mm H/C/N-P and
9 a.m. to 5 p.m. weekdays, H/C/D solids probes.
or by appointment
The facility has computational resources for processing and analyzing NMR data and obtain-
Contact: ing molecular structures. The facility provides consultation on the application of NMR to solve
Bruce Fulton, Manager research problems. NMR data can be acquired and interpreted as an analytical service. Projects
bfulton@iastate.edu larger in scope can be pursued on a collaborative basis. In the latter case, the facility will
515-294-2139 provide training and guidance for researchers to operate instruments and interpret data.
More information is available online at www.public.iastate.edu/~bfulton/nmr_home.html.
Web: www.public.iastate.
edu/~bfulton/nmr_home.html
2 Biotechnology Service FacilitiesChemical Instrumentation Facility
Location:
Suite 1234 Hach Hall
2438 Pammel Drive
T he Chemical Instrumentation Facility (CIF) has more than five million dollars’ worth of
analytical instrumentation available to faculty, graduate students, industry and other edu-
cational institutions. The staff of five highly-qualified professionals supports university research
Ames, IA 50011-1079 through maintaining and updating analytical equipment and by providing application support
and user training. Services available to the research community include the following:
Hours:
24 hours a day, seven days Electronics Repair Service
a week; professional help The CIF has restarted a small electronics repair service for evaluation and repair of various
available from 8 a.m. to 5 p.m. laboratory equipment. Electronics diagnostic equipment is also available for checkout.
weekdays
Magnetic Resonance
Contacts: Five NMR spectrometers of varying frequencies from 400 to 600 MHz are available. Chemical
Sarah Cady, Manager structure and dynamics are determined by using this technique. A fully-equipped EPR system
sdcady@iastate.edu also is available. Equipment located in the facility includes a Bruker Avance II 600 NMR with
515-294-5805 cryoprobe-solution and solids capability; Bruker Avance III 600 and DRX-500 and Agilent MR-
400 (solution) NMRs; and a Bruker NEO-400 equipped with flow cell, reaction kinetics, and
Shu Xu LC-NMR capabilities. There is also a fully-equipped Bruker E-580 FT-EPR.
NMR Lab
sxu@iastate.edu Mass Spectrometry
515-294-5975 The mass spectrometry lab is equipped to provide both low- and high-resolution GC-MS on
mixtures, pyrolysis GC-MS, and high-resolution measurements for determining the elemen-
Kamel Harrata tal composition of pure samples and multi-component mixtures. GC x GC capability is now
Mass Spec Lab offered. Electrospray and APCI are used routinely for the ionization of medium and higher
kamel@iastate.edu molecular weight compounds, including synthetic organics and compounds of biological
515-294-5544 origin. Two dedicated LC-MS instruments are available for routine work and special projects.
MS-MS (parent-daughter relationship) experiments are routine. Equipment located in the facil-
Arkady Ellern ity includes an Agilent 5973 GCMS equipped with a pyrolysis unit, an Agilent 7250 GC-QTOF,
X-ray Lab a Shimadzu LCMS2020, and an Agilent 6540 QTOF LC MSMS.
ellern@iastate.edu
515-294-6419 Preparative HPLC
The Agilent PrepStar SD-1 preparative HPLC system is capable of flows ranging from 1-2 mls
Brett Boote per minute (analytical) to 200 mls per minute (preparative). The system includes two pumps,
Spectrophotometry Lab and a dual-wavelength detector, fraction collector and complete data system for instrument control
Electronics Repair and data processing. Both semi-prep (9 mm i.d.) and preparative (21 mm i.d.) columns are
bboote@iastate.edu available for use for an additional fee.
515-294-5975
X-ray Diffraction
Web: www.cif.iastate.edu A completely equipped X-ray diffraction laboratory provides instrumentation for the study
of the molecular structures of small molecules and powders. The equipment includes Bruker
APEX II CCD and VENTURE D8 single-crystal diffractometers equipped with low-temperature
devices, and a Rigaku Ultima 4 powder diffractometer available for general use. The facility’s
crystallographer provides complete reports that are suitable for publication.
Spectrophotometry
A variety of spectrophotometers are available for routine use in the facility. These instruments
provide fingerprint spectra for characterizing and identifying compounds. These instruments
currently include a Bruker Tensor 37 FT-IR, a Hewlett-Packard HP-8453 Diode Array UV-Vis,
a Cary 100 Bio double beam UV-Vis, a Jasco J-710 circular dichroism spectrophotometer, a
Horiba DeltaFlex TCSPC fluorometer, a steady-state Perkin Elmer LS55 fluorescence spectrom-
eter, and a benchtop polarimeter.
Scanning Electron Microscopy
The facility’s JEOL JSM-IT200 scanning electron microscope with X-ray energy dispersive spec-
troscopy for elemental analysis is capable ofIsothermal Titration Calorimetry (ITC) A Microcal ITC is available to study the thermodynamics of chemical reactions initiated by the addition of a binding compo- nent. It is often used to characterize biomolecular interactions. Elemental Analysis A Thermo Scientific FlashSmart combustion elemental analyzer is available for sample submission or for investigator use. Normally, the instrument is configured for carbon, hydrogen, nitrogen, and sulfur, but oxygen content also can be analyzed upon special request. Thermal Studies A Netzsch STA449F1 TGA/DSC instrument is coupled to a mass spectrometer and an FTIR. This allows simultaneous differential scanning calorimetry, thermogravimetric, and evolved gas studies to be performed in one measurement. Particle Size and Zeta Potential A Malvern Zetasizer-Nano is used to measure particle size and zeta potential. Several cell types are available. The instrument includes an MPT-2 titrator and degasser so that zeta-potential may be studied as a function of titratable parameters. 4 Biotechnology Service Facilities
Comparative Pathology Core Service
Location:
2712 Veterinary Medicine
1800 Christensen Drive
Ames, IA 50011-1134
T he Comparative Pathology Core Service (CPC) provides pathology support services and
consultation for investigators working with animal models of human and animal diseases.
The veterinary pathologists in the CPC provide gross and microscopic tissue examination and
clinical pathology evaluation.
Hours:
By appointment, Monday Services offered include, but are not limited to, tissue processing, staining of all kinds, imag-
through Friday ing, immunohistochemistry, RNAscope (ISH), image analysis via HALO software, morphomet-
rics, microscopic lesion scoring, and more, depending on the needs of the researcher.
8 a.m. to 5 p.m.
The CPC provides service to investigators both within and outside Iowa State University and
Contact: can assist with a broad range of preclinical studies in a wide range of species. Studies that CPC
Rachel Phillips has completed include toxicology, vaccine studies, infectious disease, nutritional physiology,
rlp79@iastate.edu neoplastic disease, musculoskeletal disease and developmental disorders.
515-294-0953
Rates are based on histopathology lab services and assistant scientist/pathologist’s time. More
Website: detailed information and assistance with experimental study design can be obtained by
https://vetmed.iastate.edu/ contacting Rachel Phillips at rlp79@iastate.edu.
vpath/services/comparative-
pathology-core-service
2022 5Center for Crops Utilization Research
Location:
Administration
1041 Food Sciences Building
T he Center for Crops Utilization Research (CCUR) is a resource to assist Iowa State research-
ers and external businesses with developing new processes, products and markets for corn,
soybean, and other crops. CCUR strives to add value to grain and plant materials by conduct-
536 Farm House Lane ing grant and contract research; offering short courses, workshops, seminars and training; per-
Ames, IA 50011-1054 forming analytical testing, pilot plant processing, and consumer evaluation services; providing
technical consulting services; and offering small-business incubator services. The center has
Pilot Plant Office state-of-the-art laboratory and pilot plant processing and analytical equipment.
1955 Food Sciences Building
536 Farm House Lane The pilot plant facilities include a 5,000-square-foot wet-processing pilot plant (e.g., soy
Ames, IA 50011-1054 protein isolation, corn wet milling); a 2,600-square-foot dry-processing pilot plant (e.g., dry
corn milling, drying, grinding, sieving); a 3,000-square brewery facility, and various process
development and analysis laboratories (e.g., chromatography, grain analysis, vegetable oil refin-
Hours: ing, baking, process development).
Fall, winter, spring:
8 a.m. to 5 p.m., weekdays The sensory facility has the space and capabilities for both trained and consumer panels.
Summer: 7:30 a.m. to 4 p.m., Consumer evaluation includes preference and acceptability tests in private booths. Trained
weekdays panel evaluation, such as descriptive analysis, takes place in one of two focus group rooms and
Other hours by arrangement private booths.
Contacts: A 58-person capacity theater and conference facilities are available for technology transfer
Zhiyou Wen activities.
Interim Director
wenz@iastate.edu CCUR administers and works closely with the Iowa State University Fermentation Facility.
515-294-0426
Rates are available on the facility’s website.
Jordan Funkhouser,
Pilot Plant Manager
jfunk@iastate.edu
515-294-3572
Web: ccur.iastate.edu/
6 Biotechnology Service FacilitiesCryo-Electron Microscopy Facility
Location:
0112-0114 Molecular
Biology Building,
T he Cryo-Electron Microscopy (Cryo-EM) Facility of the Office of Biotechnology provides
research infrastructure and supports interdisciplinary research in structural biology. The
facility was developed with support from the Roy J. Carver Charitable Trust.
2437 Pammel Drive
Ames, IA 50011-1079 The Cryo-EM Facility provides training, education and support in all aspects of cryo-EM
sample preparation; data collection and single-particle data analysis; and structure
Hours: 9:00 a.m. to 5:00 p.m. determination.
Weekdays
The facility houses a Thermo Fisher Scientific 200 kV Glacios microscope equipped with a K3
Contacts: Direct Electron Detector for high-resolution cryo-EM and Vitrobot for sample preparation. The
facility has dedicated 250-TB local storage and 28 GPUs (seven nodes) for computation.
Puneet Juneja, Manager
pjuneja@iastate.edu
The facility services are provided on a fee-for-service basis and include sample preparation,
515-294-1335 Cryo-EM grid screening, and data collection. The facility also provides letters of support and
assistance with grant writing.
Web:
https://www.biotech.iastate. Rates are available at https://www.biotech.iastate.edu/cryoem/.
edu/cryoem/
2022 7DNA Facility
Location:
1184 Molecular Biology
Building, 2437 Pammel Drive
T he Office of Biotechnology’s DNA Facility performs Sanger DNA sequencing, next-gen
(Illumina and Oxford Nanopore) sequencing, automated fluorescent genotyping, DNA
synthesis and primer walking, DNA and RNA characterization, quantitative and digital PCR,
Ames, IA 50011-1079 and plant tissue grinding services. Clients may submit orders through the OnCore LIMS
system. This software allows clients to track order progress and automatically notifies them
when data are ready to download.
Hours:
By appointment, 7:30 a.m. Sanger Sequencing
to 5 p.m. weekdays
(www.dna.iastate.edu/sangersequencing.html)
Sequencing samples are run on the DNA Facility’s Applied Biosystems 3730xl DNA Analyzer
Contacts: which provides up to 900 bases of usable sequence data per reaction and is capable of
David Wright, processing twelve 96-well sample plates in a 24-hour period. DNA is routinely sequenced as
Interim Manager plasmid or PCR products while lambda, cosmid or BAC DNA sequencing services are also
515-294-4705 available. Custom primers can be used with all types of templates and sequencing results are
wrightd@iastate.edu generally returned within 24 hours after sample receipt. The facility also provides a primer
walking service (www.dna.iastate.edu/primerwalking.html) and a four-color sequence data
DNA Sequencing printout can be provided.
dnaseq@iastate.edu
Clients with large numbers of similar samples may achieve significant cost savings by
utilizing the High Throughput Sanger sequencing service. This service is optimized for
DNA Synthesis well-characterized DNA samples in a 96-well plate. Clients are encouraged to submit a few
isu_oligo@iastate.edu test samples through the standard Sanger sequencing service to access primer and template
quality prior to committing to sequencing 96-well plates.
Web: www.dna.iastate.edu
NextGen Sequencing
(www.dna.iastate.edu/nextgensequencing.html)
(https://dna.biotech.iastate.edu/nextgen-libraryprep.html)
(https://dna.biotech.iastate.edu/genomic-dna-extraction.html)
The DNA Facility operates both an Illumina NovaSeq and an Illumina MiSeq. These
instruments can produce up to 3 billion (Novaseq) and 25 million (MiSeq) sequencing reads
per flow cell, respectively, making them ideally-suited for many applications, including
ChIP-seq, genome resequencing, mRNA and small RNA sequencing, and 16S amplicon
sequencing. The facility provides library preparation services for these applications as well
as many of the chromium applications, including single cell RNA, linked reads, single cell
ATAC-seq, and immunoprofiling. Clients can choose various read lengths and either single
or paired-end sequencing. Depending on the application, more than 384 samples can be
multiplexed per flow cell.
The facility also operates an Oxford Nanopore GridION sequencer. This instrument is
capable of providing very long reads. Depending on the quality of the genomic DNA,
average read lengths of 13,000 base pairs or more can be achieved with some reads greater
than 150,000 base pairs per run, producing a total of 10 to 15Gb of data per flow cell. It is
well-suited for de novo assemblies, for full-length transcript sequencing, or in conjunction
with existing short-read data to fill gaps in assemblies. The facility provides high molecular
weight DNA extraction and library preparation services for most Oxford Nanopore
applications.
Genotyping
(www.dna.iastate.edu/genotyping.html)
The facility can process a number of marker types including SSRs, AFLPs, SNPs, ISSRs, and
T-RFLPS using an Applied Biosystems 3500 DNA analyzer to electrophorese samples and
collect image data. Each sample can have as many markers as the client can identify. The
data are analyzed using Applied Biosystems’ GeneMapper Analysis software, and electronic
files are provided via the facility server.
8 Biotechnology Service FacilitiesOligonucleotide Synthesis Quantitative and Digital PCR
(www.dna.iastate.edu/oligosynthesis.html) (www.dna.iastate.edu/quantitativepcr.html)
(https://dna.biotech.iastate.edu/primerwalking.html) (https://dna.biotech.iastate.edu/biomarkhd.html)
The DNA synthesis service synthesizes DNA oligomers in two The DNA Facility maintains a quantitative, real-time PCR
scales – 50-nmol and 200-nmol – and can make modified instrument, the Applied Biosystems StepOnePlus, and will
oligomers such as the fluorescent primers used in genotyping accept jobs on a ready-to-run basis. Applications include
applications. Oligos are synthesized using a BioAutomation gene expression studies, validation of microarray data, allelic
MerMade-192 DNA synthesizer. In addition, primer design for discrimination, SNP analysis, and screening for GMOs. For
primer walking sequencing projects is available at www.dna. clients with projects that require a higher throughput, the
iastate.edu/primerwalking.html. facility has an available Fluidigm® BioMark™ HD system. This
instrument uses microfluidic technology to accurately combine
reagents and samples to perform thousands of individual
DNA and RNA Characterization nanoliter-scale PCR or genotyping assays in a single run.
(www.dna.iastate.edu/bioanalyzer.html)
(https://dna.biotech.iastate.edu/covaris-ultrasonicator.html)
The facility has two instruments available to assist with nucleic Plant Tissue Grinding Service
acid sizing and quantification. The Agilent Bioanalyzer is (https://dna.biotech.iastate.edu/tissuegrinding.html)
best-suited to smaller numbers of samples with sizes up to The DNA Facility provides a plant tissue and seed grinding
12kb in length. The Advanced Analytical Fragment Analyzer service to on-campus clients using the Spex CertiPrep
can quickly and economically process larger numbers of Genogrinder 2000. Fresh or lyophilized plant tissues
samples up to 200Gbp in length. The facility also has a Qubit are ground in a 96-well format using ball bearings in
instrument for DNA/RNA quantitation, a Covaris ME220 for individual 1.1-ml bullet tubes and seed are ground in ½ inch
DNA shearing, a Sage Blue Pippin for DNA size fractionation polycarbonate tubes. This instrument is operated on a ready-
and a plate reader capable of running a number of different to-run basis. New users will receive initial instrument training.
assays. These instruments are available for client use (training After completion of training, all users will be responsible for
available) or as facility-run. running their own samples.
2022 9Doubled Haploid Facility
Location:
1204 Agronomy Hall
716 Farm House Lane
T he Doubled Haploid Facility (DHF) offers the production of doubled haploid lines in
maize to Iowa State scientists, as well as external academic researchers and breeders. The
facility uses laboratories, greenhouses and nurseries provided by the Department of Agron-
Ames, IA 50011-1051 omy. The development of homozygous lines is an important, but time-consuming, process
in plant breeding and research. The induction and subsequent doubling of haploids is an
Hours: efficient alternative to generate homozygous offspring in two generations.
8 a.m. to 5 p.m. weekdays
The DHF also develops new inducer lines with higher adaptation to the growing conditions
of the Midwest, additional selectable markers for haploid detection, and for use in specialty
Contacts: crops such as popcorn.
Thomas Lübberstedt
Professor-in-Charge The mission of the DHF is to:
thomasl@iastate.edu
515-294-5356 • provide expertise and service in the production of doubled haploid lines in maize
515-294-3163 (fax) • improve the technology in order to get higher success rates and lower costs
• teach and train scientists and students
Ursula K. Frei,
Research Scientist The production of doubled haploid lines consists of three main steps:
ufrei@iastate.edu
515-294-2756 • haploid induction by pollination with inducer lines
• selection for haploid kernels
Web: • doubling of haploid plants and subsequent seed production
https://www.doubledhaploid.
biotech.iastate.edu/ Haploid Induction
The DHF uses their own haploid inducing lines (ISURF #04099) for all induction crosses.
BHI306 and closely related lines have the R1-nj kernel color marker and the Pl1 root color
marker for visual haploid selection. They also have the Ga1 allele, that overcomes partial dent
sterility often present in popcorn backgrounds. All induction crosses are done by hand-polli-
nation. The induction rate in field corn, popcorn and sweet corn varies in the range of 8-15%
depending of the genetic background of the female/donor population.
Haploid Kernel Selection
Kernels with a haploid or F1 embryo can be distinguished by means of the expression of the
dominant anthocyanin marker gene R1-nj. The expression of this gene provides an anthocyan
pigmentation of the embryo and the endosperm. Kernels with a pigmented endosperm and a
non-pigmented embryo are selected as haploids. In materials difficult to select based on R1-
nj, additional selection for haploids can occur at the seedling stage using the red root marker
(Pl1).
Doubling
Haploid plants are sterile. In order to produce seed on these plants, the genomes of cell lines
developing into generative organs (tassel, ear) have to be initiated to double their genomes.
This is usually done by applying a chemical that interferes with cell division. At the DHF,
seedlings at the two to three leaf stage are injected with a colchicine solution (Eder et al.
2002). On average 20-25% of the haploid plants show male fertility restoration, and 10-15%
of the seedlings can be successfully self-pollinated.
The DHF collaborates with a winter nursery in Chile, to be able to return DH lines within
one year.
Spontaneous Haploid Genome Doubling (SHGD)
Haploid genome doubling is still the major bottleneck in DH technology. Introducing spon-
taneous haploid genome doubling into the donor pool might help in the future to improve
doubling rates and eliminate the need for germinating, treating and transplanting haploid
seedlings. A germplasm with high spontaneous haploid genome doubling is now available.
The trait is conferred by one major QTL. Donor lines for SHGD are available for licensing.
Contact the facility for more information.
10 Biotechnology Service FacilitiesFermentation Facility
Location:
1621 Food Sciences Building
536 Farm House Lane
Ames, IA 50011-1054
T he Fermentation Facility is designed to help researchers develop new fermentation tech-
nologies and products and provide benchtop- and pilot-scale fermentation equipment.
The facility has equipment for scaling up the production of industrially important chemicals,
(campus location) chemical feedstocks, genetically-modified organisms and enzymes.
1327 U Avenue The facility includes ten benchtop fermenters with 2-liter to 10-liter working volume capaci-
Boone, IA 50036 ties and two pilot-scale fermenters with 500-liter and 1000-liter working volume capacities.
(BioCentury Research Farm) Downstream processing equipment includes a spiral wound membrane filtration system, three
semi-continuous tubular centrifuges, a continuous disc centrifuge, a pilot scale homogenizer
(20 liters per hour), an eight-shelf freeze dryer with stoppering capability, a distillation tower, a
Hours: falling film evaporator, and a rotary steam tube dryer.
8 a.m. to 5 p.m. weekdays
The equipment is housed in the Food Sciences Building at the Iowa State University central
Contacts: campus in Ames, Iowa, and BioCentury Research Farm (BCRF) at an Iowa State University
Zhiyou Wen, Interim Director satellite campus located seven miles west of Ames, Iowa, on U.S. Highway 30.
Center for Crops Utilization
Research
wenz@iastate.edu
515-294-0426
Youjie Xu
Fermentation Specialist
youjiexu@iastate.edu
515-294-0643
Fax: 515-294-6261 or
515-294-8181
Web: www.ccur.iastate.edu/
fermentationfacility
2022 11Flow Cytometry Facility
Location:
Main Facility
1104 (Office) or 1117 (Lab) T he Flow Cytometry Facility of the Office of Biotechnology offers flow cytometric analysis
and cell sorting for a wide range of research applications. Facility personnel are trained to
assist researchers in flow cytometry experimental design, sample preparation techniques, and
Molecular Biology
Building, 2437 Pammel Drive, data analysis. Training sessions to provide general instruction in these areas are also offered in
Ames, IA 50011-1079 the facility. All facility services are open to internal Iowa State University clients, as well as to
external institutions and individuals.
Hours: Flow cytometry is used to analyze bacteria, mammalian cells, fungi, yeast, insect cells, mi-
8 a.m. to 5 p.m. weekdays, croscopic parasites, plant cells, nuclei, organelles and chromosomes. Immunofluorescence
by appointment measurements are used to provide information on intracellular and cell surface receptor densi-
ties. DNA/RNA-specific stains supply information on genome size, chromatin structure, and
cell cycle kinetics. Fluorochromes are available for quantitating a number of cellular metabolic
Contacts: functions, such as calcium mobilization and oxidative burst. Phagocytosis of fluorescently
Shawn Rigby, Manager labeled particles (beads, yeast or bacteria) can be quantified. Cell viability and concentra-
rigbysm@iastate.edu tion can be measured for mammalian cells, as well as bacteria. Fluorescent lipophilic dyes are
515-294-2472 available that have been utilized as a means of tracking cell life and tissue localization in vivo.
Levels of enzyme marker gene expression, such as beta-galactosidase, can be correlated with
Christine Deal the fluorescence intensity of cleaved substrate by flow cytometry. Intracellular protein products
Assistant Scientist can be measured by immunofluorescent labeling of fixed cells. Fluorescence in situ hybridiza-
515-294-2472 tion (FISH) techniques provide information on the mRNA expression level of a specific gene
and can be used in conjunction with flow cytometry to provide quantitative gene expression
information on a cell-by-cell basis. Necrotic versus apoptotic-mediated cell death can be dis-
Web: tinguished using flow cytometry. It is also possible to sort individual cell populations via flow
https://flow.biotech.iastate.edu cytometry, enabling researchers to separate and further characterize subpopulations of cells.
Flow Cytometry Data Acquisition and Cell Sorting
The Flow Cytometry Facility maintains two flow cytometers for data acquisition and cell
sorting: a BD Biosciences FACSAria III and BD Biosciences FACSCanto. The facility also main-
tains several computer workstations with software packages for performing off-line analysis of
flow cytometry data.
BD Biosciences FACSAria III
The BD FACSAria III is a sorting flow cytometer. The power of multi-color flow cytometry and
the highly sensitive analysis platform of this instrument allow investigators to categorize an
almost endless variety of unique cell subpopulations. Once cells of interest are identified, the
high-speed sorting platform of the FACSAria III can simultaneously isolate and collect these
target events for further study.
The FACSAria III has three excitation lasers (405, 488 and 633 nm) and is configured to detect
nine fluorescent parameters (Pacific Blue, AmCyan, FITC, PE, PE-TexasRed, PerCP-Cy5.5, PE-
Cy7, APC and APC-Cy7), as well as forward and side scatter. This gives investigators extensive
flexibility in reagent selection and experimental design. The FACSAria III has the capacity for
11-parameter detection on particle sizes ranging from 0.5 to 50 μm in diameter. Thus, viable
cell, aseptic sorts can be performed on a wide range of cell types (i.e. bacteria, mammalian leu-
kocytes, plant protoplasts, etc.). Up to four unique populations can be sorted simultaneously
into 1.5 mL microtubes, 12 x 75 mm, or 15 mL tubes. Cells can also be sorted into several
different types of tissue culture plates and slides, and both sample and collection chambers can
be maintained at pre-determined temperatures.
Digital electronics allow the FACSAria III to achieve data acquisition rates as high as 70,000
events per second. For many common sorting experiments, an event rate of 25,000 per second
will consistently attain ≥98% sort purity and ≥80% of expected yield. Higher event rate sorts
(i.e. 50,000 – 70,000 per sec.) can be achieved in many instances with minimal loss of sort
purity and only moderate loss of expected yield.
12 Biotechnology Service FacilitiesThe FACSAria III is located in the Molecular Biology Building MoFlo XDP (MBB) main facility and is operated by facility personnel only. The Beckman Coulter MoFlo XDP is a sorting cytometer with the Data acquired on the FACSAria III is loaded onto a network unique ability to sort chromosomes. Flow cytometric chromo- server which allows the remote retrieval of data files via an Eth- some classification (flow karyotyping) is routinely used in the ernet connection. The Flow Cytometry Facility also maintains a analysis of mammalian karyotypes, as well as to assess chromo- backup of user data. An appointment to schedule an experiment somal abnormalities. The MoFlo is equipped with two lasers, on the FACSAria III can be made by simply calling or emailing which allows karyotype analysis and purification of chromosomes Flow Cytometry Facility personnel. by labeling with Hoechst 33258 and Chromomycin A3. BD Biosciences FACSCanto Following purification, DNA and proteins can be extracted from The FACSCanto is a data acquisition flow cytometer that com- the sorted chromosomes for genomics and proteomics studies. bines a patented optical design, digital electronics and a novel sample injection system supporting carryover of less than 0.1%. The MoFlo is located in the Molecular Biology Building and is High-speed data processing, industry-leading sensitivity and min- operated by facility personnel only. An appointment to schedule imal sample-to-sample carryover make this instrument uniquely an experiment on the MoFlo can be made by contacting Flow suited for rare event analysis. The FACSCanto has the capacity for Cytometry Facility personnel. 16-parameter detection on particle sizes ranging from 0.5 to 50 μm in diameter, which includes leukocytes, cell lines, platelets, For more detailed information about chromosome sorting on the bacteria, multiplexed bead technologies and more. MoFlo, please contact the Flow Cytometry Facility. The FACSCanto has three excitation lasers (405, 488, and 633 Additional Laboratory Equipment nm) and is configured to detect 14 fluorescent parameters (in- A refrigerated table-top centrifuge, carbon dioxide incubators, cluding, but not limited to, FITC, PE, PE-TexasRed, PerCP-Cy5.5, biohazard hood, temperature-controlled water baths, analyti- PE-Cy7, APC, Alexa Fluor 700, APC-Cy7, BV421, BV510, BV605, cal balances, pH meter, refrigerators, freezers and a cryostorage BV650, BV711, and BV785), as well as forward and side scatter. system are available. The system can handle high sample flow rates (up to 120 mL/ min.) and fast acquisition rates (up to 10,000 events per sec.). More information is available online at https://flow.biotech.iastate. Digital electronics also facilitate compensation, with no limits to edu. inter- and intra-beam compensation, allowing post-acquisition compensation. Data files are stored raw and compensated as part of the FCS files, allowing flexibility for off-line compensation when viewing data. The FACSCanto instrument is located in the MBB main facility and is operated by facility personnel only. FACSCanto-generated data are uploaded to cloud storage which allows customers to re- trieve data files from their offices or labs via internet web browser. The Flow Cytometry Facility also maintains a backup of user data. An appointment to schedule an experiment on the FACSCanto can be made by contacting facility personnel. 2022 13
Genome Informatics Facility
Location:
448 Bessey Hall
2200 Osborn Drive
T he Genome Informatics Facility (GIF) of the Iowa State University Office of
Biotechnology provides bioinformatics services for investigators within academia,
industry and government. It serves as a centralized resource of expertise on the application of
Ames, IA 50011-4009 emerging sequencing technologies and open source software as applied to biological systems.
Its missions include integrating this knowledge into educational resources and facilitating data
analysis that transforms big data for the acceleration of understanding biological and
Hours: 8 a.m. to 5 p.m. evolutionary processes.
Monday through Friday.
Contact the facility regarding
holiday hours. Available Services
• Grant review (methods, data management plans, outreach)
• Figure generation for papers
Contact: • Experimental design review
Andrew Severin, Manager • Genome assembly and annotation
severin@iastate.edu • Transcriptome assembly and annotation
515-294-1320 • Variant calling (SNP/InDel)
• RNA-seq analysis and single cell RNA-Seq analysis
For general bioinformatic • ChIP-seq
questions or inquiries: • Proteomics analysis
gifhelp@iastate.edu • Metagenomics analysis
• Training of students, postdocs and staff
Web: Work order requests may be submitted through the GIF website: https://gif.biotech.iastate.edu/
http://gif.biotech.iastate.edu users.
www.bioinformaticsworkbook.
org To request bioinformatics support or services, please email the facility at gifhelp@iastate.edu.
Include in the email the type of organism and the types of analyses envisioned. An appoint-
ment can then be made to further discuss the project.
Free tutorials are available in the facility’s Bioinformatics Workbook located online at
www.bioinformaticsworkbook.org.
14 Biotechnology Service FacilitiesGenomic Technologies Facility
Location:
2025 Roy J. Carver T The Genomic Technologies Facility (GTF), an Iowa State core laboratory in the Plant
Sciences Institute, provides access to equipment for genomic research. In-house next
generation sequencing can be performed on the Ion Proton™ platform. One real-time
Co-Laboratory
1111 WOI Road thermocycler, a Roche LightCycler 480, is available to conduct high-throughput gene
Ames, IA 50011-1085 expression analysis. The GTF offers Agilent Bioanalyzer, Sage Science Blue Pippin, Eppendorf
AF2200 Plate Reader, Life Technologies Qubit and Qiagen BioSprint instruments for DNA/RNA
quantification, fragment size selection and DNA/RNA extraction.
Hours: By appointment
Life Technologies™ Sequencing Services (Ion Proton)
Contacts: Ion Proton Sequencing and custom library preparation can be completed in two days. Template
Lakshmi Attigala library sequences are amplified on Ion Sphere Particles and checked for quality. An enrich-
Overall Management ment step purifies the amplified template, and sequencing is performed using Ion Proton’s
lakshmi@iastate.edu semiconductor ion chips. During this process, nucleotides are sequentially incorporated into
515-294-8563 DNA, causing a hydrogen ion to be released. With the Ion Proton P1 chip, 200 bp single-end
sequencing can provide up to 80-100 million reads per run. Sequencing runs can be conduct-
For general inquiries: ed by on-campus customers on a fee-for-service basis.
gtfstaff@iastate.edu
Ion Proton preparations available include Ion Xpress Plus Fragment Library and Ion Total
RNA-Seq.
Shu-Ting Tsao
Billing Questions
After preparation, libraries are quantified to ensure proper loading of the library. Users can
sttsao@iastate.edu
quantify libraries using the GTF’s Bioanalyzer.
515-294-7209
Users are required to provide a signed and approved user agreement to facility staff before
Web: accessing facility equipment and services. After approval of technique, users can operate the
www.plantgenomics.iastate. equipment and conduct analyses themselves. Reservations to use instrumentation may be made
edu using the facility’s on-line scheduler.
2022 15Grain Quality Laboratory
Location:
3167 National Swine T he laboratory provides instrumental analyses of chemical and physical properties of grain
and other agricultural products. The services are intended to support high-throughput
genetic evaluations, surveys of grain quality and other large-scale monitoring operations. The
Research and Information
Center (NSRIC); shipping facility also calibrates various types of instruments for manufacturers and users.
address: 1111 NSRIC, 1029
North University Blvd, Chemical composition is measured with a non-destructive, near-infrared (NIRS) analyzer. For
Ames, IA 50011-3611 best accuracy, this test requires at least 500 grams of product. Sample sizes down to 150 grams
will work with corresponding reductions in accuracy.
Hours: Crop-specific Composition Tests:
By appointment during pan-
demic. Contact the Facility. • Corn: moisture, protein, oil, starch and density. Ethanol yield and feed value can be calcu-
lated from the NIR results for proximate analysis.
Contacts: • Soybeans: moisture, protein, oil, fiber, total saturates and linolenic acid. Meal and oil esti-
Connie Hardy mated processed value (EPV) can be calculated from the NIR results
chardy@iastate.edu • Soybean meal: moisture, protein, oil and fiber
515-450-8026 • Distillers grain: moisture, protein, oil and fiber
Charles R. Hurburgh, Jr., General Tests
Professor-in-Charge
tatry@iastate.edu • Seed weight
515-294-8629 • Test weight
• Other contracted services
• Adaptation of NIRS instrumentation for individual situations and specialty measurements
Web: www.iowagrain.org provided upon request
The facility’s calibration service calibrates electronic measurement equipment of various types
and trains users in quality control validation. These can be done for instrument manufacturers
or for individual users on a wide range of products and instrument types.
The lab has an in-house quality management system to verify accuracy and reproducibility of
data. Advice and set-up of quality control programs for analytical data can be provided.
Pricing and Sample Submission
Please see details at https://www.extension.iastate.edu/grain/lab
Note: Iowa State clients, please contact Connie Hardy by phone at 515-450-8026 or by
email.
16 Biotechnology Service FacilitiesRoy J. Carver
H igh Resolution Microscopy Facility
Locations:
Atomic Force, Electron and T he Roy J. Carver High Resolution Microscopy Facility (HRMF) of the Office of Biotechnol-
ogy provides a variety of instrumentation, technical assistance, consultation and training
to individuals and groups of life sciences and biotechnology researchers who want to im-
Light Microscopy
0122 Molecular Biology age biological samples by using atomic force microscopy, photomacrography, laser capture
Building, 2437 Pammel microdissection (LCM), light microscopy, scanning electron microscopy, transmission electron
Drive, Ames, IA 50011-1079 microscopy, confocal and multiphoton microscopy, lightsheet microscopy and optical worksta-
tions, cryopreservation, cytochemistry, tomography, X-ray microanalysis and image analysis.
Confocal and Multiphoton In addition to the round-the-clock availability for trained individuals, the HRMF carries out
Microscopy; Lightsheet service work for both on- and off-campus researchers. The staff of the HRMF is available for
Microscopy, Laser Capture consultation and individual help. For imaging material samples, please contact the Materials
Microdissection and Image Analysis and Research Laboratory.
Analysis
0116 Molecular Biology Atomic Force and 3D Microscopy
Building, 2437 Pammel
Drive, Ames, IA 50011-1079 • Digital Instruments Dimension™ ICON Scanning Probe Microscope
The Dimension ICON scanning probe microscope (SPM) brings together all SPM
techniques in a single platform and handles a wide range of sample sizes and types. A
Hours: rigid, low vibration construction of the Dimension ICON SPM ensures the highest quality
8 a.m. to 5 p.m. weekdays, images and measurements.
by appointment
Samples up to eight inches in diameter can be scanned in ambient air or fluids using the
Contacts: Dimension ICON SPM. The Dimension ICON SPM requires little or no sample prepara-
HRMF@iastate.edu tion, and the simple vacuum protein mounting system allows easy and convenient setup.
Curtis Mosher, Manager, Superior linearity and resolution in all three dimensions are obtained, even for large sam-
Atomic Force and 3D ples. Integrated top-view video optics with motorized zoom and 1.5 µm optical resolution
Microscopy help to quickly and easily identify areas of interest for detailed scanning. Changing scan-
515-294-6513 ning techniques, for example from Tapping Mode™ to ScanAsyst™, requires no tools.
Margie Carter, Manager, • Digital Instruments MultiMode with Tapping Mode™
Confocal and Multiphoton The MultiMode system features multiple scanners that permit the user to tailor the system
Microscopy; Lightsheet Mi- for individual research. Scanners with large scan ranges up to 120 microns on the X–Y
croscopy, Image Analysis and axes and a Z range up to 6 microns, as well as high-resolution scanners with 0.5
Laser Capture microns X–Y axes and submicron Z range, are available. The vertical-engage “JV” and
Microdissection “EV” scanners allow the tip to be positioned at any point on the surface, without adjusting
515-294-1011 for lateral movement during approach. The MultiMode is controlled with a NanoScope
IIIa controller. This controller provides 16-bit resolution on all three axes, with three
independent 16-bit digital-to-analog converters (DACs) in X and Y for control of the scan
Tracey P. Stewart, Manager,
pattern, scaling and offset. This configuration provides 16-bit resolution of the lateral
Electron and Light
scanning motion at any scan size.
Microscopy
515-294-3872
• Digital Instruments Dimension™ 3100 Scanning Probe Microscope
The Dimension 3100 is controlled with a Nanoscope IV controller. The NanoScope IV
Website: features up to ten-times-faster scanning, as well as increased functionality, bandwidth,
http://microscopy.biotech. flexibility and expandability.
iastate.edu
• Sensofar S neox Non-contact 3D Surface Profiler
The S neox uses Sensofar’s patented technology, based on a microdisplay. The ferroelectric
liquid crystal on silicon (FLCoS) microdisplay is a fast-switching device that makes the
scanning of confocal images fast and very stable with additional interferometry and focus
variation scanning options. Combining this approach with Sensofar’s high NA objectives
(0.95), vertical resolution is reduced to below 1 nm with state-of-the-art lateral resolution.
Confocal and Multiphoton Instrumentation
Confocal microscopy allows for real-time optical sectioning of fixed and living specimens, pro-
viding significant improvements in optical contrast and resolution over traditional light and
fluorescence microscopy. The facility is equipped with a Leica SP5 X MP confocal/multiphoton
microscope system with an inverted microscope front end. Technology available on this sys-
tem includes a white light laser, IR laser and optical parametric oscillator (OPO).
2022 17Researchers are no longer limited to three or four lasers at three The Hitachi FE-SEM offers excellent resolution (1.3nm at 1kV)
or four fixed wavelengths but can tune the white light laser to any and can operate at ultra low voltages (100-500 volts) to provide
wavelength between 470 and 670 nm, which has a similar effect high-resolution imaging of sensitive samples. This system has
to having 200 lasers and 200 wavelengths. This allows research- a Quorum cryo-imaging system and a Thermo Fisher Scientific
ers to select the excitation wavelength that is best suited to their Pathfinder elemental imaging/analysis (X-ray) system.
samples and will result in maximum fluorescence emission. On
the emission side, the system has an Acousto-Optical Beam Split- Ancillary equipment available for preparation of specimens for
ter (AOBS) which allows researchers to precisely set the emission electron microscopy includes a Denton critical point appara-
wavelengths they would like to capture rather than be limited tus, Cressington 208HR sputter coater, HPM-100 cryo-fixation
to preset emission ranges determined by fixed filter sets. Both of system, LEICA AFS II freeze substitution system, two LEICA UC6
these technologies help to separate fluorescence signals with close ultramicrotomes, a LEICA UC6 with cryo-sectioning system, and
excitation and/or emission spectra. a Leica glass knife maker.
Other features include faster scanning, higher resolution and in- Light Microscopy
creased sensitivity. Researchers will now be able to work with live Light microscopy instrumentation includes Zeiss AxioImager,
cells over an extended period using live cell equipment, including AxioObserver, and Macrozoom imaging systems that are fully
a heated stage with microcontainment or macrocontainment sys- automated, digital, and offer multiple fluorescent filters for
tem, active gas regulation, cell cultivation chamber and microin- multi-mode imaging, including tiling, z-stacking and time lapse.
jection system. For capturing images of fast-moving samples or The HRMF also has a Zeiss Axioplan II compound microscope
events that occur in a fraction of a second, the resonant scanner equipped with AxioCam color and B/W digital cameras and the
enables video rate scanning with speeds up to 16,000 lines per following optical modes – bright-field, phase-contrast, polarizing,
second. The system also has FRET, FRAP, timelapse, hyperspectral dark-field, fluorescence and Nomarski (DIC). In addition, this
signal separation software, 3D imaging, colocalization, decon- microscope has an Apotome for creating thin optical slices; an
volution, region of interest (ROI) scanning, brightfield and DIC Olympus stereomicroscope with reflected and transmitted light
capabilities. In addition to the white light laser, the system has a sources and a high-resolution digital color camera; dissecting
405 laser providing UV excitation and an Argon laser providing microscopes; and Olympus compound microscopes, one with
CFP excitation and extra power for bleaching. a digital camera. Microscopes with digital cameras have image
analysis capabilities.
The system allows users to easily switch between confocal and
multiphoton mode. In multiphoton mode, users can image deep- Preparative equipment and space for light microscopy include
er into their samples than is possible with confocal systems. The fixing and processing hoods; paraffin and resin embedding areas;
higher excitation wavelengths are less damaging to live samples, rotary-, cryo- and ultra-microtomes; cryostat, knife maker; knife
as well. The OPO expands the excitation range of the multipho- sharpener, Vibratome, vacuum oven, and centrifuges.
ton laser to include the red and high red ranges, enabling use of
essential fluorophores such as mCherry, DsRed2, mRFP, Texas
Red, Alexa Fluor 660 and others. Researchers are able to precisely
Lightsheet Microscopy
The Zeiss Lightsheet 7 microscope enables long-term imaging of
set the emission wavelengths they would like to capture.
whole-mount multicellular samples such as plant roots, zebrafish
and Drosophila embryos, whole brains, and organoids, as well as
A Zeiss 780 confocal microscope system is also available. This sys-
dynamic cell processes. The Lightsheet 7 uses two sources of illu-
tem has five lasers allowing excitation wavelengths of 405, 458,
mination juxtaposed at a 45-degree angle, exposing the specimen
488, 514, 561, 594, and 633 nm and is mounted on an AxioOb-
to a thin plane of light and reducing photo damage and bleaching.
server Z1 inverted microscope. It is equipped with two PMTs,
As a result, living specimens can be imaged repetitively over long
one 32-channel GaAsP detector and one transmission detector.
periods of time yielding more complete, in-depth, three-dimen-
Emission wavelengths between 290 and 750 nm can be detected.
sional images. By rotating the specimen, it is possible to image
A variety of dry, water, multi-immersion, and oil objectives rang-
virtually any plane with multiple views at different angles.
ing in magnification from 2.5x to 100x are available. Images are
acquired and analyzed using Zeiss Zen Black software.
In lightsheet microscopy, a laser is used to illuminate a very thin
section of a whole mount sample. The image is collected by a
Electron Microscopy full-frame, camera-based detector, resulting in image acquisition
Electron microscopy instrumentation includes a 200kV JEOL of a single thin slice of the sample at speeds 100 to 1,000 times
2100 scanning/transmission electron microscope (STEM) with faster than confocal point-scanning methods. As the laser sweeps
elemental analysis, tomography, and image analysis systems, and through the sample, a full 3D image is captured. The speed of
an Hitachi SU4800 field emission scanning electron microscope image acquisition offers several advantages. First, the laser only
(FE-SEM). illuminates a single plane of the sample for a very short period of
time. This results in minimal phototoxicity to live samples and
The JEOL 2100 STEM, withmental samples such as embryos. A lightsheet microscope also Laser Capture Microdissection System – LCM
is able to image more deeply into a sample, several millimeters Laser capture microdissection is a system used to identify and
as opposed to 200 microns for a confocal microscope or 800 retrieve individual cells from tissue sections. The retrieved cells
microns for multi-photon microscopy. can be used for assessment/analysis of RNA, DNA, protein and
other biochemical properties.
The system includes:
• 5x, 10x, 20x and 40x detection objectives plus clearing Instruction, Training and Assistance
objectives (1.45 and 1.53 refractive index) An Individual Module Training Program (IMTP) trains individu-
• 5x and 10x illumination objectives als to specifically process and visualize their research materials
• 405, 488, 561, and 638 nm lasers using targeted methods and instrumentation to accomplish their
• CO2, temperature and daylight controls objectives. This program allows an individual to begin the IMTP
• 2 PCO.edge 4.2 liquid cooled cameras within a short time after an initial consultation and acceptance of
• Arivis Vision 4D software for data analysis the training conditions. Costs for IMTP include technical training
• Chambers for standard size, large size and cleared samples assistance and instrument, lab and materials fees. The length of an
IMTP depends on the individual’s abilities and aptitudes and the
Molecular Printer System – Nano eNabler™ complexity of the training. Individuals should contact the HRMF
The Nano eNabler™ system is a highly-flexible molecular printer staff for further details and/or to schedule a consultation.
that can dispense minute volumes of liquid at defined positions to
create patterns of spots or lines with high spatial accuracy. This Rates are available on the HRMF website, http://microscopy.
technology enables the deposition of 1-30 um sample droplets biotech.iastate.edu.
quickly and precisely using materials such as: proteins, nucleic
acids, lipids, colloids, quantum dots, UV-curable adhesives,
etchants, and catalysts.
2022 19You can also read
