THE EFFECT OF BLA GABAA RECEPTORS IN ANXIOLYTIC-LIKE EFFECT AND AVERSIVE MEMORY DEFICIT INDUCED BY ACPA
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EXCLI Journal 2015;14:613-626 – ISSN 1611-2156 Received: February 19, 2015, accepted: March 09, 2015, published: May 11, 2015 Original article: THE EFFECT OF BLA GABAA RECEPTORS IN ANXIOLYTIC-LIKE EFFECT AND AVERSIVE MEMORY DEFICIT INDUCED BY ACPA Katayoon Kangarlu-Haghighia, Shahrbanoo Oryana, Mohammad Nasehib, Mohammad-Reza Zarrindastb,c,d,e* a Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran b Cognitive and Neuroscience Research Center (CNRC), Medical Genomics Research Center and School of Advanced Sciences in Medicine, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran c Department of Pharmacology School of Medicine, Tehran University of Medical Sciences, Tehran, Iran d Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran e School of Cognitive Sciences, Institute for Research in Fundamental Sciences (IPM), Tehran, Iran * Corresponding author: M.R. Zarrindast (zarinmr@ams.ac.ir); Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran), P. O. Box 13145-784, Tel: +9821-66402569, Fax: +9821-66402569 http://dx.doi.org/10.17179/excli2015-201 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). ABSTRACT The roles of GABAergic receptors of the Basolateral amygdala (BLA) in the cannabinoid CB1 receptor agonist (arachydonilcyclopropylamide; ACPA)-induced anxiolytic-like effect and aversive memory deficit in adult male mice were examined in elevated plus-maze task. Results showed that pre-test intra-peritoneal injection of ACPA induced anxiolytic-like effect (at dose of 0.05 mg/kg) and aversive memory deficit (at doses of 0.025 and 0.05 mg/kg). The results revealed that Pre-test intra-BLA infusion of muscimol (GABAA receptor agonist; at doses of 0.1 and 0.2 µg/mouse) or bicuculline (GABAA receptor antagonist; at all doses) impaired and did not al- ter aversive memory, respectively. All previous GABA agents did not have any effects on anxiety-like behav- iors. Interestingly, pretreatment with a sub-threshold dose of muscimol (0.025 µg/mouse) and bicuculline (0.025 µg/mouse) did not alter anxiolytic-like behaviors induced by ACPA, while both drugs restored ACPA- induced amnesia. Moreover, muscimol or bicuculline increased and decreased ACPA-induced locomotor activi- ty, respectively. Finally the data may indicate that BLA GABAA receptors have critical and different roles in an- xiolytic-like effect, aversive memory deficit and locomotor activity induced by ACPA. Keywords: ACPA, GABA, anxiety, memory, amygdala INTRODUCTION pounds (Burns, 2006). Various researches have shown that the administration of mari- Cannabis sativa (Marijuana) is common- juana affects various cognitive and non- ly used all over the world. The plant extract cognitive behaviors including impairment of composes of almost 70 cannabinoid com- spatial (Uchida et al., 2012; Wise et al., 613
EXCLI Journal 2015;14:613-626 – ISSN 1611-2156 Received: February 19, 2015, accepted: March 09, 2015, published: May 11, 2015 2009) and non-spatial learning and memory, 2003; Sah and Lopez De Armentia, anxiety-like behaviors, mood, locomotor, 2003). and euphoria experience both in animal It has been proven that CB1 cannabinoid models and human subjects (Burgdorf et al., receptors are expressed at high levels in the 2011; Kilmer et al., 2011; King et al., 2002; BLA amygdala nuclei (Herring et al., 2003; Pacula, 2011). Some reports revealed that the Marsicano et al., 2002). Expression of the endogenous cannabinoid system is critically CB1 protein is limited to a definite and no- linked to the extinction of aversive memories ticeable subpopulation of GABAergic inter- (Marsicano et al., 2002). Endocannabinoids neurons corresponding to large cholecysto- are thought to be retrograde messengers re- kinin-positive cells (Jazi et al., 2009). In- leased by neurons to modulate release of depth and comprehensive analysis has shown neurotransmitters (Kreitzer and Regehr, that CB1 receptors exist presynaptically on 2001; Nicolle et al., 2001; Ohno-Shosaku et cholecystokinin-positive axon terminals, al., 2001; Wilson and Mogil, 2001). Three which establish symmetrical GABAergic main cannabinoid receptors have been identi- synapses with their postsynaptic targets fied so far as CB1, CB2, and CB3 (non-CB1 (Azad et al., 2003; Katona et al., 2001). In and -CB2), which are engaged in canna- the mammals brain, γ-aminobutyric acid binoids’ functions (Mackiewicz et al., 2006; (GABA) is the most abundant inhibitory Ryberg et al., 2008). CB1 receptors are plen- neurotransmitter (Nicoll et al., 1990), work- tifully expressed in the central nervous sys- ing by means of different receptor types: the tem regions such as the hippocampus, amyg- ionotropic GABAA and GABAC receptors dala, cerebellum and cortex (Davies et al., (both of which activate Cl− currents) and the 2002; Pertwee and Ross, 2002; Wilson et al., metabotropic GABAB receptor (G protein 2002). CB2 receptors mostly are expressed coupled receptor). The present study, in ac- peripherally rather than in brain tissues. It is cordance with the above mentioned data, was believed that neuropsychological functions designed to examine the role of BLA GA- of endocannabinoids are related to CB1 re- BAergic (GABAA receptors) system on the ceptors. CB1 is mainly expressed in the ACPA (selective CB1 cannabinoid receptor amygdala (Katona et al., 2001; McDonald agonist), which causes emotional amnesia in and Mascagni, 2001), an essential part and the EPM test-retest protocol in mice. component of the limbic circuitry. The amygdala is an integral part in controlling MATERIALS AND METHODS the emotional behavior such as conditioned fear, anxiety (McKernan and Shinnick- Animals Gallagher, 1997), and pain perception Male albino NMRI (Institute Pasture, (Gauriau and Bernard, 2002; Paulson et al., Iran) mice weighting 27-30 g (9-10 week 2002). old), were applied. The animals were kept Amygdala nuclei mainly are classified under a 12/12-h light-dark cycle, with light into the three groups: beginning at 0700 h and at a controlled tem- 1) the deep or basolateral group, which in- perature (22 ± 2 c). They had free access to cludes the lateral nucleus, the basal nu- food and water. The animals were housed 10 cleus, and accessory basal nucleus used per cage (45cm × 30cm × 15cm). Eight ani- as auxiliary and helping nucleus; mals were used in each experiment. Each an- 2) the superficial or cortical-like group, imal was only used once. All procedures in which consists of cortical nuclei and nu- this investigation are in accordance with the cleus of the lateral olfactory tract; and guide for the Care and Use of Laboratory 3) the centromedial group composed of the Animals as adopted by the Ethics Committee medial and central nuclei (Faber and Sah, of Faculty of Science, Tehran University (357: November 2000). 614
EXCLI Journal 2015;14:613-626 – ISSN 1611-2156 Received: February 19, 2015, accepted: March 09, 2015, published: May 11, 2015 Drugs injection unit was connected by polyethylene Ketamine and xylazine (Alfasan Chemi- tubing to a 1 µl Hamilton syringe. The for- cal Co., Woerden, Holland) were applied for ward movement of a small air bubble inside animal anesthesia. Muscimol (GABAA re- the polyethylene tubing interposed among ceptor agonist), bicuculline (GABAA recep- the upper end of needle and the microsyringe tor antagonist) and ACPA (CB1 cannabinoid was taken as evidence of drug flow. The in- receptor agonist) have been used in this jection needles were left in place for an addi- study that purchased from Tocris, Bristol, tional 60 s to allow diffusion after which the UK). All drugs were dissolved in sterile stylets were reinserted into the guide cannu- 0.9 % saline, just before the experiment, ex- lae (Ebrahimi-ghiri et al., 2012; Nasehi et al., cept for bicuculline. Bicuculline was dis- 2012; Zarrindast et al., 2011a). solved in 1 drop of glacial acetic acid using a Hamilton microsyringe, then made up to a Apparatus and behavioral testing volume of 5 ml with sterile 0.9 % saline and We applied a wooden elevated plus-maze then diluted to the required volume. Musci- (EPM) apparatus set up 50 cm above the mol and bicuculline were administered into floor which included two oppositely posi- the BLA of amygdala at volume of 0.6 µl/ tioned open-arms (50 × 10 cm) and two en- mouse (0.3 µl/site). The control animals re- closed arms (50 × 10 × 40 cm), surrounded ceived saline or vehicle. The timing of drugs by1cm high Plexiglas ledge so that to pre- administration was defined as per our pilot vent falls. The junction area of the four arms results and the previous studies (Chegini et (central platform) measured 10 × 10 cm al., 2014; Yousefi et al., 2013). (Carobrez and Bertoglio, 2005; Zarrindast et al., 2010, 2011b). The EPM test is used to Surgical procedures and microinjections assess anxiety and memory processes in ro- All surgical procedures were organized dent models of CNS disorders. under ketamine-xylazine (100 mg/kg keta- Findings demonstrate that aversive learn- mine-5 mg/kg xylazine) anesthesia. Cannu- ing and memory may be studied at the same lae were implanted with bilateral 27-gauge time as anxiety in rodents exposed to the stainless steel cannulas into either the BLA EPM test/retest. Animals retested in the of amygdala. Drugs were injected into the EPM avoid exploring the open spaces, dis- amygdala (coordinates from bregma as fol- playing a clear enclosed arm preference with lows: AP = -0.7 mm, ML = ± 2.7, DV = 3. 8 a low percentage of entries and time spent in (Paxinos and Franklin, 2001). Skull cap was the open arms relative to their respective lev- made from dental acrylic. Finally, stainless el on testing. The aversive and fear-inductor steel wires were inserted with plastic caps in- nature of the open arms represents a useful to each cannula to prevent any debris from tool for the study of aversively motivated entering the brain and to maintain patency of learning processes in the EPM. For example, the hollow metal cylinder. The injecting learning and memory have been studied in needle extended 1 mm beyond the tip of the the EPM through avoidance to open-arms in cannulas, reflecting the ultimate desired the retest session. The different analysis in- depth of the apparatus and the actual depth dicated that this response of further avoid- the infusion needle reached during antago- ance to open-arms is gradually acquired nist or buffer infusion. Following surgery all throughout testing, and is thought to reflect animals were allowed 1 week to recover the retrieval of the aversive memory related from surgery and get cleared from anesthet- to the initial EPM exploration (Chegini et al., ics effect. For drug infusion, animals were 2014; Valizadegan et al., 2013). gently restrained in hand and the stylets were Mice were left undisturbed to the testing removed from the guide cannulae and re- room 1 hour prior to the test so that to adapt placed by 27-gauge injection needles. Each to the testing environment. The mice were 615
EXCLI Journal 2015;14:613-626 – ISSN 1611-2156 Received: February 19, 2015, accepted: March 09, 2015, published: May 11, 2015 individually placed in the center of the maze (10 ml/kg, i.p.) and ACPA (0.0125, 0.025, facing a closed arm and allowed 5 min of and 0.05 mg/kg, i.p.) 15 minutes before test- free exploration. Experiments were under a ing. To inquire possible carry-over drug ef- low light (40-lux), during the day phase, be- fects on aversive learning, treated groups tween 9:00 and 14:00 h. During this 5 min, were retested in the EPM 24 h later un- the percentage of open arm time and open drugged. arm entries were calculated as follows: (a) %OAT (the ratio of time spent in the Experiment 3: the effect of pretest open arms to total time spent in any microinjections of muscimol and bicuculline arms×100); on open-arm exploratory-like behaviors (b) %OAE (the ratio of entries into open induced by ACPA arms to total entries×100) and CAE To supply evidence that possible interac- (close arms entries as a relative pure in- tion of GABAA BLA receptors with explora- dex of locomotor activity). tory-like behaviors induced by ACPA, the These behaviors were recorded by a video drugs infusion were made before EPM test- camera while a monitor and a computer- ing for anxiety-like behavior assessment. In recording system were installed in an adja- these experiments the animals received sa- cent room. Raw data were used to manually line (0.6 µl/mouse, 4 groups), sub-threshold calculate these behaviors. Experiments were dose of muscimol (0.025 µg/mouse, 4 performed by someone blind to doses of groups) and bicuculline (0.025 µg/mouse, 4 drugs and statistical results. groups) intra-BLA, 5 min before testing. Furthermore, these animals also received sa- Experimental design line (10 ml/kg, i.p.) and sub-threshold and Experiment 1: effect of pretest micro- effective doses of ACPA (0.0125, 0.025 and, injections of muscimol and bicuculline on 0.05 mg/kg, i.p.) 15 min before testing. In open-arm exploratory-like behaviors order to look into the possible side-effects of To substantiate that the microinjection of intra-BLA drugs on aversive learning, treat- drugs into BLA involves in anxiety, the drug ed groups were retested in the EPM 24 h lat- infusion took place before EPM testing. In er un-drugged. the present experiment nine groups of ani- mals received saline (0.6 µl/mouse, 3 Verification of cannulae placements groups), vehicle (0.6 µl/mouse, 3 groups), After the completion of the experimental muscimol (0.025, 0.05, 0.1, and 0.2 µg/ sessions, each animal was eliminated with an mouse) or bicuculline (0.025, 0.05, 0.1, 0.2 overdose of chloroform. Animals received and 0.4 µg/mouse), 5 min. before testing. In intra-BLA injection of ink (0.3 l/side; 1 % order to investigate possible after-effect in- aquatic methylene blue solution). The brains tra-BLA drugs effects on aversive learning were then removed and fixed in a 10 % for- during test day to aversive memory in retest malin solution for 10 days before sectioning. day, treated groups were retested in the EPM Sections were analyzed to find out the loca- 24 h later un-drugged. tion of the cannulae aimed for bilateral BLA. The cannulae placements were checked by Experiment 2: using the atlas of Paxinos and Franklin effect of pretest microinjections of ACPA on (2001). Data which were obtained from ani- open-arm exploratory-like behaviors. mals with injection into the specified sites, To provide information on the impact of outside these regions were not taken into the ACPA on anxiety, the drug infusion hap- consideration for the analysis. pened before EPM testing. In this experi- ment 4 groups of animals received saline 616
EXCLI Journal 2015;14:613-626 – ISSN 1611-2156 Received: February 19, 2015, accepted: March 09, 2015, published: May 11, 2015 Statistical analysis with incorrectly positioned cannulae tips Given the normality of distribution and were excluded. the homogeneity of variance, the results were statistically evaluated using the repeated The effect of pretest microinjections of measure and two-way analysis of variance muscimol into BLA on open-arm explorato- (ANOVA), in which mean ± S.E.M was em- ry-like behaviors ployed for the comparison of outcomes be- Repeated measure and post-hoc analysis tween experimental groups and their corre- demonstrated that intra-BLA injection of sponding controls. Where F-value was sig- muscimol at dose 0.1 and 0.2 µg/mouse in- nificant, one-way analysis of variance creased %OAT (Figure 1; Panel 2A) and (ANOVA) and post-hoc analysis (Tukey’s %CAE (Figure 1; Panel 2B) but not CAE test) were performed. Differences with (Figure 1; Panel 2C) in the retest day, while P < 0.05 between groups were considered these interventions did not alter all behaviors statistically significant. in the test day (Figure 1; Panels 1A, 1B and 1C). In conclusion, the data uncovered that RESULTS muscimol did not induce any effect on anxie- Histology ty behaviors, while impaired aversive For the statistical analyses, we included memory acquisition. All the experimental the data only from animals with correct can- repeated measure results are summarized in nulae implants (320 animals). 27 animals the Table 1. Figure 1: Open-arms exploratory behavior following pretest microinjections of muscimol (Panels 1 and 2) and bicuculline (Panels 3 and 4) into BLA. After 24 h, all groups were retested in the EPM un- drugged. %OAT (A); %OAE (B) and CAE (C). Values are expressed as mean±S.E.M (n=8 in each group). **P < 0.05 different from respective saline group in the panel 1. +P < 0.05, ++P < 0.01 and +++P < 0.001 different from control saline group in Panel 2. ψ < 0.05 different from the saline group in the Panel 4. 617
EXCLI Journal 2015;14:613-626 – ISSN 1611-2156 Received: February 19, 2015, accepted: March 09, 2015, published: May 11, 2015 Table 1: The table describes repeated measure analysis with P values for the effect of GABAA agonist and antagonist on exploratory-like behaviors. Final results Day-Dose inter- Experiments Behaviors Day effect Dose effect conclusion for action effect each experiment Repeated measure analysis F(1,35) P F(4,35) P F(4,35) P results for muscimol microinjection into the BLA %OAT 64.25 0.0005 12.234 0.0005 10.15 0.0005 Muscimol into (between Panel 1 and 2 of %OAE 6.511 0.001 15.342 0.0005 5.978 0.001 BLA impaired Figure 1) aversive memory CAE 2.6545 0.087 3.477 0.0921 3.054 0.0857 acquisition Repeated measure analysis F(1,42) P F(5,46) P F (4,46) P results for bicuculline micro- %OAT 8.45 0.0005 4.657 0.001 4.021 0.0005 Bicuculline into injection into the BLA (be- tween Panel 3 and 4 of Fig- %OAE 6.245 0.001 5.346 0.001 3.542 0.052 BLA decreased locomotor activity ure 1) CAE 3.546 0.046 1.645 0.1648 4.679 0.041 The effect of pretest microinjections of sition. All the experimental repeated measure bicuculline into BLA on open-arm explora- results are summarized in the Table 2. tory-like behaviors Repeated measure and post-hoc analysis The effect of pretest microinjections of demonstrated that intra-BLA injection of muscimol and bicuculline on open-arm ex- bicuculline at dose 0.4 µg/mouse decreased ploratory-like behaviors induced by ACPA CAE (Figure 1; Panel 4C) but did not alter Two-way ANOVA and post-hoc analysis %OAT (Figure 1; Panel 4A) and %CAE showed that pretest intra-BLA injection of (Figure 1; Panel 4B) in the retest day. The sub-threshold dose of muscimol (0.025 µg/ interventions did not alter any behaviors in mouse) or bicuculline (0.025 µg/mouse) po- the test day (Figure 1; Panels 3A, 3B and tentiated %OAT (Figure 2 Panel 4A for 3C). In conclusion, the data showed that muscimol and Figure 2 Panel 6A for bicucul- bicuculline did not induce any effect on anx- line) and %OAE (Figure 2 Panel 4B for iety behaviors and aversive memory ac- muscimol and Figure 2 Panel 6B for bicucul- quired. All the experimental repeated meas- line) induced by ACPA in the retest day. The ure results are summarized in the Table 1. interventions showed that muscimol and bicuculline did not %OAT (Figure 2 Panel The effect of pretest intraperitoneal 3A for muscimol and Figure 2 Panel 5A for injections of ACPA on open-arm bicuculline) and %OAE (Figure 2 Panel 3B exploratory-like behaviors for muscimol and Figure 2 Panel 5B for bi- Repeated measure and post-hoc showed cuculline) induced by ACPA in the test day. that intraperitoneal injection of ACPA at the Interesting data showed co-administration of highest dose (0.05 mg/kg) increased %OAT muscimol or bicuculline with ACPA decree- (Figure 2; Panel 1A) and %CAE (Figure 2; sed and increased respectively, locomotor Panel 1B) but not CAE (Figure 2; Panel 1C) activity both test and retest days (Figure 2 in the test day. The same interventions in the Panel 3C and 4C for muscimol and Figure 2 retest day showed that ACPA increased Panel 5C and 6C for bicuculline). Finally, %OAT (Figure 2; panel 2A at doses 0.025 the data revealed that the main effect of and 0.05 mg/kg) and %CAE (Figure 2; Panel muscimol and bicuculline is on ACPA-in- 2B at dose 0.05 mg/kg) but not CAE (Figure duced locomotor activity rather than anxiety 2; Panel 2C). In conclusion, the data demon- and aversive memory. All the experimental strated that ACPA induced anxiolytic-like repeated measure results are summarized in effect and impaired aversive memory acqui- the Table 2. 618
EXCLI Journal 2015;14:613-626 – ISSN 1611-2156 Received: February 19, 2015, accepted: March 09, 2015, published: May 11, 2015 Figure 2: Panels 3 and 4 for muscimol and Panels 5 and 6 for bicuculline show the effect of intra- BLA pre-testing injection of sub-threshold dose muscimol and bicuculline on open-arms exploratory- like behavior induced by both sub-threshold and effective doses of ACPA. After 24 h, all groups were retested in the EPM un-drugged which showed by %OAT (A), %OAE (B) and CAE (C). Values are expressed as mean±S.E.M (n = 8 in each group). *P < 0.05 and **P < 0.01 different from saline group in the Panel 1. ψψψP < 0.001 different from saline group in Panel 2. For panels 3 φφ < 0.01 and φφφ < 0.001 are compared to respective group in the Panel 1, while δ < 0.05, δδ < 0.01 and δδδ < 0.001 are compared to the respective group in the Panel 2. DISCUSSION 2001, 2005; Canli et al., 2000), thus, it seems that amygdala and the hippocampal system It has been presumed that in the events jointly play a critical role in the emotional which are connected to the feelings and emo- memory improvement (Dolcos et al., 2004). tions, the amygdala (a major brain region) Moreover, emotional situation including regulates hippocampal formation activity for aversion and fear, may improve or may driving information recording to cortical are- weaken memory formation (McGaugh, as. For instance, BLA activation into inacti- 2004). Since the existing animal models of vation situation (i.e. damage) change learning and memory have a limited ability memory from the event in favor of the essen- to detect the effect of drugs on anxiety and tial part to visual details (Adolphs et al., fear memory, as measured by these models, 619
EXCLI Journal 2015;14:613-626 – ISSN 1611-2156 Received: February 19, 2015, accepted: March 09, 2015, published: May 11, 2015 Table 2: Repeated measure analysis with P values for the effect of ACPA by itself and two-way ANO- VA results for the effect of GABAA agonist and antagonist on exploratory-like behaviors induced by ACPA. Treatment-dose Final results con- Treatment Experiments Behaviors Dose effect interaction ef- clusion for each effect fect experiment Two-way ANOVA analysis F(1,56) P F(3,56) P F(3,56) P results for the effect of muscimol microinjection in- %OAT 2.314 0.0845 2.154 0.152 2.351 0.129 to the BLA on exploratory- Muscimol reduced like behaviors induced by %OAE 2.945 0.164 2.364 0.124 1.872 0.0961 locomotor activity ACPA in the test day (be- induced by ACPA tween panel 1 and 3 of Fig- CAE 3.214 0.042 3.134 0.048 3.045 0.05 ure 2) Two-way ANOVA analysis F(1,56) P F(3,56) P F(3,56) P results for the effect of muscimol microinjection in- %OAT 6.342 0.001 7.125 0.001 3.164 0.05 Muscimol potentiat- to the BLA on exploratory- ed and decreased like behaviors induced by %OAE 3.021 0.041 4.12 0.04 5.215 0.01 aversive memory ACPA in the retest day (be- deficit and locomo- tween panel 2 and 4 of Fig- tor activity induced ure 2) CAE 4.284 0.034 2.641 0.07 4.12 0.01 by ACPA, respec- tively Two-way ANOVA analysis F(1,56) P F(3,56) P F(3,56) P results for the effect of bicuculline microinjection %OAT 2.31 0.081 2.745 0.084 1.97 0.245 into the BLA on explorato- Bicuculline in- ry-like behaviors induced %OAE 2.54 0.154 1.87 0.86 1.548 0.154 creased locomotor by ACPA in the test day activity induced by (between panel 1 and 5 of ACPA CAE 7.25 0.001 5.21 0.001 21.354 0.001 Figure 2) Two-way ANOVA analysis F(1,56) P F(3,56) P F(3,56) P results for the effect of bicuculline microinjection %OAT 5.31 0.001 5.02 0.001 4.32 0.001 Bicuculline potenti- into the BLA on explorato- ated and increased ry-like behaviors induced %OAE 3.25 0.01 3.04 0.01 2.91 0.048 aversive memory by ACPA in the retest day deficit and locomo- (between panel 2 and 6 of tor activity induced Figure 2) CAE 5.85 0.001 6.65 0.001 8.14 0.0005 by ACPA, respec- tively the exact subject matter and points may be The effect of ACPA on open-arm misinterpreted and misunderstood. There- exploratory behaviors in native mice fore, elevated plus maze (EPM) task is an at- subjected to the EPM tempt to assess the effects of drugs on anxie- The present results show that, intra- ty, learning, and memory happening at the peritoneal infusion of selective CB1 canna- same time in rodents (Asth et al., 2012). The binoid receptor agonist, ACPA, make anxio- justification for utilizing the EPM in testing lytic-like behaviors appear in the EPM. anxiety relies on the natural tendency of an- Moreover, anxiolytic effects of ACPA re- imals to avoid dangerous situation when they vealed in retest day. These results propose an face height and open spaces (Chegini et al., impairment of aversive memory acquisition 2014; Zarrindast et al., 2010). on testing presented itself in ACPA-treated groups. Meanwhile, the drug did not alter lo- comotor activity in the test and retest days. It has been showed that cannabinoids have several effects on the cognitive and non- 620
EXCLI Journal 2015;14:613-626 – ISSN 1611-2156 Received: February 19, 2015, accepted: March 09, 2015, published: May 11, 2015 cognitive behaviors such as short-term nabinoid receptors are one of the major re- memory deficit, mood alterations, increased ceptors in the emotional learning and body awareness, decreased attention, sleepi- memory and its neural plasticity process ness and discoordination (Court, 1998; (Laviolette and Grace, 2006; Marsicano et Heishman et al., 1997). In terms of anxiety- al., 2002) and they have high expression in like behaviors, it appears that the effects of BLA and medial prefrontal cortex (mPFC) cannabinoid agonists on this phenomenon (McDonald and Mascagni, 2001). are complex and often contradictory and conflicting in both humans and animals. In The effect of BLA GABAA agents on open- the anxiety animal model these agents in- arm exploratory behaviors duced dose-dependent regulation which The results showed that intra-BLA infu- seems the animal is strongly affected by en- sion of muscimol and bicuculline did not al- vironmental context. For instance, low doses ter anxiety-like behaviors. Interestedly, fur- of nabilone (Onaivi et al., 1990), CP55, 940 ther analyses showed that muscimol im- (Marco et al., 2004) and Delta9-tetrahydro- paired memory formation and locomotor ac- cannabinol (Berrendero and Maldonado, tivity. A large number of studies have re- 2002) as CB1 cannabinoid receptor agonists ported that amygdale plays an important role induced anxiolytic-like effects in the EPM in anxiety-like behaviors (Roozendaal et al., and light–dark tasks. On the other hand, the 2009); specifically amygdale BLA nucleus CB1 knockout mice also showed an anxio- (Wang et al., 2011). The amygdale BLA and genic-like behavior in the EPM and social lateral nucleus receives GABAergic neuron interaction task (Haller et al., 2002; Uriguen input from the raphe nucleus (Smith and et al., 2004). A recent study reported that Porrino, 2008). This system and its receptors cannabinoid agonists at high and low doses are involved in the regulation of cognitive induced opposite effects on cognitive behav- and non-cognitive behaviors including anxie- iors (Moreira and Wotjak, 2010). For in- ty-like behaviors, emotional memory, loco- stance, high and low doses of these com- motor activity, attention, biorhythms, food pounds induced anxiogenic- and anxiolytic- intake and body temperature (Abrams et al., like behaviors, respectively. The effects can 2005; Bonn et al., 2013; Holmes, 2008; be blocked by CB1 cannabinoid antagonists Kriegebaum et al., 2010). GABA is the ma- (Haller et al., 2007). jor inhibitory neurotransmitter in the mam- A large body of evidence shows that the mals CNS, including the brain stem. The endocannabinoidergic system plays a crucial GABA induces most of its effects through role in physiological mechanisms of learning activation of either GABAA or GABAB re- and memory (Lichtman et al., 2002; ceptors. Synaptically released GABA acti- Marsicano et al., 2002). For example, sys- vates postsynaptic GABAB receptors, which temic injections of Δ9-THC, anandamide or increase the membrane permeability to chlo- intra-hippocampal injections of WIN55212-2 ride, evoking a hyper-polarizing inhibitory impair memory acquisition, consolidation, postsynaptic current (IPSC). Inside the syn- and recall in rodents (Costanzi et al., 2004; apse, the concentration of GABA to a rela- Mishima et al., 2001; Nasehi et al., 2010). In tively high level (milimolar range) is in- terms of emotional memory, several brain creased by synaptic release (Cathala et al., regions including hippocampus, amygdala, 2005; Farrant and Nusser, 2005). The short and cortex with high density of CB1 receptor current which is named as “phasic” inhibi- expression have critical role in emotional tion happens as a result of the synaptic re- behavior regulation (Viveros et al., 2005). It lease of GABA from presynaptic terminals. seems that the endocannabinoidergic system But new detailed examinations have revealed plays a major role in aversive memory ex- that GABA released from presynaptic termi- tinction (Marsicano et al., 2002). CB1 can- nals can escape, or spillover, from the synap- 621
EXCLI Journal 2015;14:613-626 – ISSN 1611-2156 Received: February 19, 2015, accepted: March 09, 2015, published: May 11, 2015 tic cleft, or there may be a spillover of damide following a foot shock after hearing GABA from the synaptic cleft, leading to ac- in the amygdale (Marsicano et al., 2002). tivations of the receptors either at the syn- Moreover, the contribution of amygdale en- apse or distant from it (Cathala et al., 2005; docannabinoids in the extinction of aversive Farrant and Nusser, 2005). On the contrary memories has been also proposed (Azad et our results two previous studies showed that al., 2004). It has been reported that in stress- GABAA receptor activation induced anxio- ful stimuli, as well as rewarding experiences, lytic-like behaviors (Naseri et al., 2014; mediate changes in the expression level of Rezayat et al., 2005), Many drugs such as the CB1 receptor specifically in GABAergic benzodiazepines, barbiturates and alcohols terminals (Rossi et al., 2008; Yamodo et al., seem to elicits their effects via GABAA re- 2010). It is well worth considering that, the ceptors, (Morrow, 1995). GABAA receptors stress-mediated regulation of the GABAergic are ligand-gated heterooligomeric complexes CB1 receptor has been postulated as a com- comprising distinct subunits. Pharmacologi- pensatory mechanism needed to restore the cal studies have highlighted the crucial role balance between GABAergic and glutama- of the GABAergic system in the regulation tergic neurotransmission in emotional home- of anxiety. For instance, using pentylene- ostasis (Ruehle et al., 2012). CB1 canna- tetrazole (a GABAA receptors blocking binoid receptors are expressed in GABAer- agent) is shown to induce anxiety-like ef- gic terminals of the amygdale (Haring et al., fects. On the contrary, using benzodiaze- 2007). Thus, the endocannabinoidergic sys- pines (with increasing effect on GABAergic tem can modulate GABAergic transmission transmission) induce anxiolytic-like effect through regulating the activity of afferents (Kalueff and Nutt, 1997). The data from into GABA producing neurons (Haj- bicuculline-included experiments may sug- Dahmane and Shen, 2005), and through di- gest that under normal conditions, the BLA rectly modulating the functions of a subset of GABAA receptors are not necessary for the GABAergic neurons (Haring et al., 2007). In anxiety-like behaviors and aversive memory the connection of cannabinoidergic and GA- formation. Some other studies have reported BAergic systems interactions, some reports similar effects of intra-CA1 bicuculline on have mentioned that cannabinoids and their memory retention (Chegini et al., 2014; receptor agonists such as anandamide and Zarrindast et al., 2002), spatial change and ACPA inhibit the uptake of GABA into the non-spatial novelty detection (Yousefi et al., cortical synaptosomes and this may happen 2013). through reducing the activity of the uptake energy source Na+/K+-ATPase (Steffens and The effect of BLA GABAergic system on Feuerstein, 2004). Thus, using of canna- open-arm exploratory behavior induced by binoid receptor agonist, blocks respective ACPA transporters and finally increases GABA The data uncovered that muscimol and level in different brain regions (Köfalvi, bicuculline did not alter anxiolytic-like be- 2007). In vivo study showed GABAA recep- haviors induced by ACPA, while both drugs tors have a critical role in modulation effect restored ACPA-induced amnesia. Interest- of cannabinoid (Beinfeld and Connolly, ingly muscimol or bicuculline increased and 2001). For example it showed that blocked decreased ACPA-induced locomotor activi- of GABAA receptors by bicuculline com- ty, respectively. This mainly showed that pletely restored Δ9-THC-induced deficits in there is a dealing mechanism between anxie- both the Morris water maze working- ty and cannabinoid level, to the extent that memory task and an alternation T-maze task anxiety events increased endocannabinoid (Varvel et al., 2005). However a study tone level for the reduction of anxiety phe- showed that microinjection of the GABAA nomenon, for instance, the increase of anan- agonist muscimol into the central nucleus of 622
EXCLI Journal 2015;14:613-626 – ISSN 1611-2156 Received: February 19, 2015, accepted: March 09, 2015, published: May 11, 2015 the amygdala but not into the BLA nucleus Asth L, Lobao-Soares B, Andre E, Soares Vde P, of the amygdala, reduced the antinociceptive Gavioli EC. The elevated T-maze task as an animal model to simultaneously investigate the effects of effects of systemic WIN55, 212-2 (Manning drugs on long-term memory and anxiety in mice. et al., 2003), Rea and et al. (2013) demon- Brain Res Bull. 2012;87:526-33. strated that CB1 receptors in the BLA facili- tate the expression of fear-conditioned anal- Azad SC, Eder M, Marsicano G, Lutz B, Zieglgans- berger W, Rammes G. Activation of the cannabinoid gesia, through a mechanism which is likely receptor type. 1 decreases glutamatergic and GABA- to involve the modulation of GABAA signal- ergic synaptic transmission in the lateral amygdala of ing. the mouse. Learn Mem. 2003;10:116-28. CONCLUSION Azad SC, Monory K, Marsicano G, Cravatt BF, Lutz B, Zieglgansberger W, et al. Circuitry for associative In conclusion, the findings of the present plasticity in the amygdala involves endocannabinoid study proposed that ACPA induced anxiolyt- signaling. J Neurosci. 2004;24:9953-61. ic-like effect and aversive memory deficit. Beinfeld MC, Connolly K. Activation of CB1 canna- Furthermore, muscimol and bicuculline did binoid receptors in rat hippocampal slices inhibits not and restored anxiolytic-like effect and potassium-evoked cholecystokinin release, a possible aversive memory deficit by ACPA, respec- mechanism contributing to the spatial memory defects produced by cannabinoids. Neurosci Lett. 2001;301: tively. Moreover, muscimol or bicuculline 69-71. increased and decreased locomotion by AC- PA, respectively. It seems that the main ef- Berrendero F, Maldonado R. Involvement of the fect of GABAA in ACPA-induced behaviors opioid system in the anxiolytic-like effects induced by is on locomotor activity rather than anxiety Delta(9)-tetrahydrocannabinol. Psychopharmacology (Berl). 2002;163:111-7. and aversive memory behaviors. Bonn M, Schmitt A, Lesch KP, Van Bockstaele EJ, Acknowledgments Asan E. Serotonergic innervation and serotonin recep- The authors thank the Iran National Sci- tor expression of NPY-producing neurons in the rat lateral and basolateral amygdaloid nuclei. Brain Struct ence Foundation (INSF) for providing the fi- Funct. 2013;218:421-35. nancial support for the project. Burgdorf J, Zhang XL, Weiss C, Matthews E, Dister- Conflicts of interest hoft JF, Stanton PK, et al. The N-methyl-D-aspartate receptor modulator GLYX-13 enhances learning and There are no conflicts of interest. memory, in young adult and learning impaired aging rats. Neurobiol Aging. 2011;32:698-706. REFERENCES Burns JW. Arousal of negative emotions and Abrams JK, Johnson PL, Hay-Schmidt A, Mikkelsen symptom-specific reactivity in chronic low back pain JD, Shekhar A, Lowry CA. Serotonergic systems patients. Emotion. 2006;6:309-19. associated with arousal and vigilance behaviors following administration of anxiogenic drugs. Neuro- Canli T, Zhao Z, Brewer J, Gabrieli JD, Cahill L. science. 2005;133:983-97. Event-related activation in the human amygdala associates with later memory for individual emotional Adolphs R, Denburg NL, Tranel D. The amygdala's experience. J Neurosci. 2000;20:RC99. role in long-term declarative memory for gist and detail. Behav Neurosci. 2001;115:983-92. Carobrez AP, Bertoglio LJ. Ethological and temporal analyses of anxiety-like behavior: the elevated plus- Adolphs R, Tranel D, Buchanan TW. Amygdala maze model 20 years on. Neurosci Biobehav Rev. damage impairs emotional memory for gist but not 2005;29:1193-205. details of complex stimuli. Nat Neurosci. 2005;8:512- 8. Cathala L, Holderith NB, Nusser Z, DiGregorio DA, Cull-Candy SG. Changes in synaptic structure under- lie the developmental speeding of AMPA receptor- mediated EPSCs. Nat Neurosci. 2005;8:1310-8. 623
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