Lab Recharge Jump start 2018 - GE and Fisherbrand join forces to deliver tools for Life Science applications - Fisher Scientific
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Lab Recharge
Jump start 2018
Isolation and
Amplification
Cell Culture
Sample Prep
Purification
Analysis
GE and Fisherbrand join forces to
deliver tools for Life Science applications
Table of Contents
Isolation and Amplification 3
Nucleic Acid Isolation ...................................................................................................................................................................................... 3
PCR*......................................................................................................................................................................................................................... 3
Why Choose Sera-Mag Beads? .................................................................................................................................................................... 3
Sample Collection and Storage ................................................................................................................................................................... 3
Nucleic Acid Purification and Clean-Up ................................................................................................................................................... 4
Nucleic Acid Amplification ............................................................................................................................................................................ 5
Fisherbrand........................................................................................................................................................................................................ 6
Cell Culture 8
Serum ..................................................................................................................................................................................................................... 8
Specialty Water................................................................................................................................................................................................... 8
Process Liquids and Buffers.......................................................................................................................................................................... 8
Fisherbrand......................................................................................................................................................................................................10
Sample Preparation 12
Membrane Filters ............................................................................................................................................................................................13
Syringe Filters....................................................................................................................................................................................................13
Syringeless Filters ...........................................................................................................................................................................................14
Glass Microfibre Filters .................................................................................................................................................................................14
Thimbles ..............................................................................................................................................................................................................14
Lab Essentials ...................................................................................................................................................................................................15
Fisherbrand .....................................................................................................................................................................................................16
Protein Purification Information Base 18
Protein Purification Information Base ................................................................................................................................................... 18
ÄKTA start ..........................................................................................................................................................................................................19
Affinity Chromatography .............................................................................................................................................................................20
Size Exclusion Chromatography ................................................................................................................................................................23
Desalting/Buffer Exchange .........................................................................................................................................................................23
Fisherbrand .....................................................................................................................................................................................................24
Analysis 27
Gel Electrophoresis ........................................................................................................................................................................................26
Western Blotting Detection .......................................................................................................................................................................27
Transfer ...............................................................................................................................................................................................................27
Labelling ..............................................................................................................................................................................................................27
Imaging ................................................................................................................................................................................................................28
Selection Guides ..............................................................................................................................................................................................29
Fisherbrand......................................................................................................................................................................................................30
2
Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
Isolation and Amplification Savings
up to
25%
Encapsulation Magnetite Core
Nucleic Acid Isolation Why Choose Sera-Mag™ Beads?
illustra™ RNASpin Kits Sera-Mag is the trusted brand for magnetic bead technology
to the scientific community for over 30 years. For robust and
illustra RNAspin RNA Isolation Kits are designed for rapid extraction
reproducible results choose from:
of high-quality RNA (up to 100 µg) from a wide range of sample types.
• Streptavidin coated – isolation of biotinylated targets
• Fast and simple
such as PCR products, Oligos, and Ab
• Easy to use
• Universal bead reagent for increased throughput and
• Available in Mini and 96-well formats precision in immunoassays
• Carboxylate-modified – optimized for covalent coupling
PCR* of proteins and nucleic acid isolation
• Oligo (dT) – excellent for isolating mRNA from a variety
illustra PuReTaq Ready-To-Go™ PCR Beads of sources
• Neutravidin coated – streptavidin blocked surfaces may
Premixed, predispensed, single-dose reactions optimized for
help reduce non-specific binding in some applications
performing standard PCR.
•
•
High-quality PuReTaq DNA polymerase and high-purity reagents
Verified for real-time PCR
Sample Collection and Storage
• Long-term ambient-temperature stable Easy sample collection and storage using FTA™ and
• Available in 96-well plates and Hot Start Mix CloneSaver™ cards.
Ordering information
GE catalogue Fisher Scientific Fisher Scientific
Item description Pack size number number promo price (€)**
Isolation and Amplification
illustra RNAspin 96 Kit (4 × 96 Well Plates) 4 × 96 rxn 25-0500-75 11763299 828,00
illustra RNAspin Mini Kit 50 rxn 25-0500-71 11545065 172,50
illustra PuReTaq RTG PCR 0.2 mL MWP 96 rxn 27-9557-01 10380395 108,17
illustra PuReTaq RTG PCR 0.2 mL Hinged Tube with Cap 96 rxn 27-9559-01 10678095 109,28
illustra Nucleon BACC1 50 rxn RPN8501 10145834 121,50
illustra Nucleon BACC2 50 rxn RPN8502 10637265 237,75
illustra Nucleon HT 50 rxn RPN8509 10499724 192,75
illustra Hot Start Mix RTG 0.5 mL 100 rxn 28-9006-46 10126624 99,52
illustra Hot Start Mix RTG 0.2 mL 96 rxn 28-9006-53 10544625 128,25
Sera-Mag Oligo (dT) Coated Magnetic Particles 1 mL 38152103011150 12305793 83,81
Sera-Mag SpeedBeads Streptavidin-Blocked Magnetic Particles 1 mL 21152104011150 11814982 234,75
Sera-Mag Streptavidin 5 mL 30152105010150 11805022 470,25
SpeedBead Streptavidin 1 mL 66152104011150 11869912 168,00
Sera-Mag SpeedBeads Neutravidin-Coated Magnetic Particles 5 mL 78152104010150 11528692 537,00
CloneSaver Card, 96 Well Format 5/pk WB120028 11989424 122,25
FTA Elute Micro Card 100/pk WB120410 11994416 319,20
Indicating FTA Elute Micro Card 100/pk WB120411 11904426 373,50
** Please enter the promo code 13007 to get access to the promo prices when you order.
3
Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
Double-stranded PCR product
A
Unincorporated G C
primers and dNTPs
T
+ Exonuclease K
Single-stranded DNA digested 3' to 5', releasing
deoxyribonucleoside 5' monophosphates (dNMPs).
5'
5' dNMPs
Alkaline Phosphatase
Catalyzes the dephosphorylation of unincorporated nucleotides and dNMPs
A
G C Nucleosides & Pi
T
5' dNMPs
Intact PCR product ready for
sequencing, cloning, genotyping etc.
Nucleic Acid Purification and Clean-Up
illustra NAP Columns illustra ExoProStar™
These disposable columns are prepacked with Sephadex™ G-25 DNA ExoProStar 1-Step PCR and Sequence Reaction Clean-Up reagent
Grade and require only gravity to run. They allow rapid and efficient provides a simple, single-step method for removing unincorporated
purification of DNA and oligonucleotides in less than 15 min. primers and nucleotides prior to downstream analysis. illustra
• Useful for small-scale purification, desalting and buffer exchange Exonuclease I and Alkaline Phosphatase enzymes are combined
• Available in three sizes depending on sample volume: 0.5 mL, in a single, stable mix requiring just one pipetting step to establish
1 mL or 2.5 mL the clean-up reaction. 30 min protocol.
ExoProstar S is an enzymatic PCR clean up technology with
illustra MicroSpin™ G-25 Columns Exonuclease I and Shrimp Alkaline Phosphatase to remove
unincorporated primers and dNTPs. For maximum flexibility it
These are designed for the rapid purification of DNA for use in a
comes in two separate tubes; just two simple pipetting steps
wide range of applications, including desalting, buffer exchange,
needed to prepare the reaction. Complete heat inactivation of
and removal of unincorporated nucleotides from end-labelled
the enzymes within 10 min. Fast 15 min protocol and scalable
oligonucleotides.
for different reaction sizes. Easy to automate.
• Prepacked with Sephadex G-25 DNA Grade and pre-equilibrated
in distilled water containing 0.05% Kathon CG/ICP Biocide
• Requires less than 4 min from sample application to collection illustra GFX™ PCR DNA and Gel Band
of purified product Purification Kit
Isolate and concentrate DNA fragments from PCR mixtures,
agarose gel bands, and enzyme digests in under 10 min.
• Flexible elution volume for different DNA concentrations
• Highly pure DNA is ready for direct use in sequencing, PCR,
labelling, restriction enzyme digestion, and cloning
Ordering information
GE catalogue Fisher Scientific Fisher Scientific
Item description Pack size number number promo price (€)**
Clean-Up/Purification
illustra NAP-5 Columns, 0.5 mL 50 columns 17-0853-02 10218284 300,00
illustra NAP-10 Columns, 1 mL 50 columns 17-0854-02 10208564 308,80
illustra NAP-25 Columns, 2.5 mL 50 columns 17-0852-02 10186464 364,00
illustra MicroSpin G-25 Columns 50 columns 27-5325-01 11743309 146,25
illustra ExoProStar-1 Step 100 rxn US77702 12124082 65,10
illustra ExoProStar S 100 rxn US79010 15209764 51,44
illustra GFX PCR DNA and Gel Band Purification Kit 100 rxn 28-9034-70 10536295 150,40
illustra GFX 96 PCR Purification Kit (10 × 96 Well Plates) 10 × 96 rxn 28-9034-45 11753329 1.089,00
** Please enter the promo code 13007 to get access to the promo prices when you order.
4
Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
Random hexamer Phi29 DNA polymerase
Nucleic Acid Amplification
illustra TempliPhi™ DNA Amplification Kit illustra Single Cell GenomiPhi™ DNA
Prepare DNA sequencing templates from circular DNA for cycle Amplification Kit
sequencing, cloning, and transformation in 4 to 6 hours. This kit combines the novel capabilities of Phi29 DNA polymerase
• Use simple protocol to reduce time, labor, and quantity of with an optimized formulation to amplify genomic DNA from
consumables needed for template preparation - workflow 1-1000 cells without background interference.
enables easy automation • Amplifications can be completed in less than 3 hours with less
• Use amplified DNA directly for cycle sequencing without than 15 min hands-on time
purification • Whole genome amplification of genomic DNA from as little as
• Amplify DNA from bacterial or M13 liquid cultures, colonies, a single cell
plaques, glycerol stocks, or purified circular (plasmid or M13) DNA
illustra GenomiPhi V2 DNA Amplification Kit
This kit offers highly efficient and representative amplification of
the whole-genome.
• Outperforms PCR-based whole genome amplification techniques
• Quick and simple automation-friendly protocol; no thermal
cycler required
• Yields high-quality DNA (e.g., for high-throughput genotyping,
hybridization, and DNA archival)
Ordering information
GE catalogue Fisher Scientific Fisher Scientific
Item description Pack size number number promo price (€)**
Amplification
illustra TempliPhi DNA Amplification Kit 100 rxn 25-6400-10 10794562 171,75
illustra Single Cell GenomiPhi DNA Amplification Kit 25 rxn 29-1081-07 15301652 285,75
illustra Single Cell GenomiPhi DNA Amplification Kit 100 rxn 29-1080-39 15311652 1.026,75
illustra GenomiPhi V2 DNA Amplification Kit 100 rxn 25-6600-31 10628505 342,00
illustra RTG GenomiPhi V3 DNA Amplification Kit 96 rxn 25-6601-96 12967160 389,25
** Please enter the promo code 13007 to get access to the promo prices when you order.
5
Focus on Fisherbrand™ for your PCR Clean Up Needs
Our compact and versatile centrifuges, vortex mixers and dry baths as well as essential bioreagents, microplates, tubes, tube racks,
cell strainers and gloves are an excellent accompaniment to help you with your PCR protocols.
Fisherbrand for PCR Clean Up
Baths, Block Digital Dry, Isotemp™ Centrifuge, Mini
• Digital controls and display of time and temperature • Small footprint and quiet operation
• Advanced internal temperature sensing probe for outstanding • UV resistant housing of SnapSpin™ rotors for quick and easy change
temperature accuracy and control • Compact ergonomic design and footprint
• Precise temperature control with PID circuit • Max speed 6000 rpm/2000 xg
• Temperature calibration enables to offset the temperature to • Weighted rubberised base removes potential movement and
desired value further dampens any noise output
• Timer to accurately monitor the heating time • Warranty: One year
• Wide range of interchangeable aluminum alloy heat blocks
provide versatility and allow for easy cleaning and disinfecting Centrifuge, Midi
• Powder-coated steel body construction ensures durability • Variable speed setting 500 rpm to 12 500 rpm, in 100 rpm
• Built-in over-temperature protection device warrants sample increments
and user safety • Variable timer 1 s to 30 min, in 1 s increments
• Unit must be ordered with block(s) • Selectable digital display of speed in RCF or rpm
• Low noise, vibration and a small footprint
Microcentrifuges, 17/17R • Imbalance and tilt safety cut out
• Quick spin button
• Capacity: 48 mL (24 × 1.5/2 mL tubes)
• Supplied complete with two rotors and a universal power
• Refrigerated and ventilated models
adapter for worldwide voltages
• Maximum speed/RCF: 13 300 rpm/17,000 xg
• Compact footprint and brushless motor for quiet operation and
• Adjustable 99 min timer and quick-spin feature for short runs
low vibration
• Supplied with rotor and biocontainment lid
• Back lit display showing time, speed and operational
information on the same screen
• UV resistant plastic, increased useful life under harsh light conditions
Ordering information
Fisher Scientific Fisher Scientific
Item description Pack size number price (€)
Fisherbrand Equipment
Isotemp Digital Dry Baths/Block Heaters, Single Block 1 15387928 329,00
Isotemp Digital Dry Baths/Block Heaters, Dual Block 1 15397928 373,00
Isotemp Digital Dry Baths/Block Heaters, Four Block 1 15317938 438,00
Standard Mini Centrifuge, 6000 rpm 1 15358266 243,00
Midi Centrifuge, 500 to 12 500 rpm 1 12972041 575,00
Microcentrifuge ACCUSPIN MICRO 17 with Rotor 24 × 1.5/2.0 mL, Ventilated 1 11516873 1.680,00
Microcentrifuge ACCUSPIN MICRO 17R with Rotor 24 × 1.5/2.0 mL, Refrigerated 1 11526873 4.010,00
6
Tubes, PCR Timer, Four Channel Alarm, Traceable™
• Polypropylene tubes with attached caps • Set four separate channels independently from 99 h, 59 min,
• Available with flat or dome caps 59 sec to one second
• Compatible with standard 0.2 mL or 0.5 mL thermal cycler blocks • Large high display allows viewing from a distance
• Thin walled • Return display to previously programmed countdown time at the
• DNAse and RNAse free touch of a button
• Extra-loud, high decibel alarms for 1 min or can be silenced manually
• Resolution: 1 second and accuracy: 0.01%
• Dimensions 70 × 64 × 13 mm
• Supplied with clip, stand, magnet, battery, Traceable™ certificate
Ordering information
Fisher Scientific Fisher Scientific
Item description Pack size number promo price (€)**
Fisherbrand PCR Plastics
96-Well Semi-Skirted PCR Plates Natural 25 12104122 91,85
96-Well Low-Profile, Skirted PCR Plates, Natural 25 12154112 82,58
96-Well Non-Skirted PCR Plates, Natural 25 12104112 80,73
96-Well PCR Rack, Assorted Colors (Blue, Green, Pink, Yellow, Orange), Polypropylene 5 11710344 58,37
Traceable Big-Digit Timer/Stopwatch, 4 Channels 1 11745863 33,68
PCR Tube 0.2 mL, No Cap, PP, Natural 250 11849241 101,38
PCR 8-Caps Strip, Domed, PP, Natural 250 11859251 37,03
PCR 8-Caps Strip, Flat, PP, Natural 250 12184102 34,80
PCR 8-Tubes 0.2 mL Strip, Flat Cap, Natural 250 12179770 178,71
PCR Tube 0.2 mL, Flat Cap, PP, Natural 1000 12134102 44,69
PCR Tube 0.5 mL, Flat Cap, PP, Natural 1000 12194142 34,15
PCR Tube 0.2 mL, Domed Cap, PP, Natural 1000 11899221 41,76
Microcentrifuge Tube Racks, Polypropylene, 80 Well Assorted 5 11728084 42,37
(Blue, Green, Pink, Yellow, Orange)
Polypropylene, Rota Rack™ Duo, Tube Rack 1 11304095 35,97
Microcentrifuge Tubes with Locking Snap Cap 0.5 mL 500 15422545 25,00
Microcentrifuge Tubes with Locking Snap Cap 1.5 mL 500 15432545 15,44
Microcentrifuge Tubes with Locking Snap Cap 2.0 mL 500 15442545 21,16
Cellstrainer 40 µm 50 11587522 64,80
Cellstrainer 70 µm 50 11597522 65,04
Cellstrainer 100 µm 50 11517532 64,76
Fisher BioReagents™ PCR Reagents
Taq DNA Polymerase 100U 10449693 22,46
PCR Nucleotides Mix, 10 mM of Each 200 µL 10163103 30,39
PCR Nucleotides Mix, 10 mM of Each 1 mL 10091743 112,68
** Please enter the promo code 13007 to get access to the promo prices when you order.
7
Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
Cell Culture Savings
up to
60%
Serum Specialty Water
FetalClone™ Products Molecular Biology Grade water is purified,
HyClone™ FetalClone products are an engineered alternative to FBS. These high performance tested, and certified to have no detectable
products provide high lot-to-lot consistency, low endotoxin and hemoglobin levels, and enzyme activity in DNase, RNase, and
IgG levels comparable with those found in FBS. protease assays.
• Significantly low levels of endotoxins, with
HyClone FetalClone I HyClone FetalClone II HyClone FetalClone III maximum allowable limit of 0.05 EU/mL
• For multiple cell culture, buffer
• Optimized for the growth • Optimized for the • The most widely
preparation, DNA/RNA purification, DNA
of hybridoma cells growth of CHO cells applicable FetalClone
sequencing and libraries, gene mapping,
• Demonstrated cell and derivatives product, designed to
recombinant DNA and cloning, PCR
growth performance • Improved cell growth include fibroblasts in
analysis, Northern and Southern blots
equivalency to FBS performance relative its applications
• Sterile filtered using to FBS • Offers the same basic
three sequential 100 nm • Offering the same formulation found in Process Liquids
(0.1 µm) pore size
rated filters. Each lot
basic formulation
as FetalClone I, with
FetalClone II, with
additional growth factors
and Buffers
undergoes extensive additional growth and supplements A range of high quality ready made buffers
quality performance and factors and supplements • Improved cell growth is available to save your time.
analytical testing performance relative See selection of Whatman syringe filters
to FBS on pages 13 and 14.
Ordering information
GE catalogue Fisher Scientific Fisher Scientific
Item description Pack size number number promo price (€)**
Serum
HyClone FetalClone I 500 mL SH30080.03 10780245 207,60
HyClone FetalClone II 500 mL SH30066.03 10326762 207,60
HyClone FetalClone III 500 mL SH30109.03 10570083 207,60
Specialty Water
Water, WFI Quality, Meets Current USP Monograph Criteria for WFI Packaged in 1000 mL SH30221.10 10317002 8,05
Bulk for Commercial use Elsewhere. Not for Diagnostic or Therapeutic Use
Water, Cell Culture Grade, Endotoxin-free (< 0.005 EU/mL), Deionized, Distilled, 1000 mL SH30529.03 10011342 7,22
0.1 µm Sterile Filtered
Water, Molecular Biology Grade, DNase, RNase, Protease Free (no Activity 1000 mL SH30538.03 10307052 8,87
Within Assay Detectability Limits), Deionized, Distilled, 0.1 µm Sterile Filtered
Process Liquids and Buffers
Earle's Balanced Salt Solution (EBSS), 1X, with Calcium, Magnesium, Phenol Red 500 mL SH30029.02 10306382 5,94
Dulbecco’s Phosphate Buffered Saline (DPBS), 1X, with Calcium, Magnesium, 500 mL SH30264.01 10798134 6,09
without Phenol Red
Hank’s Balanced Salt Solution (HBSS), 1X, with Calcium, Magnesium, Phenol Red 500 mL SH30030.02 10225362 5,94
Phosphate Buffered Saline (PBS), 1X, 0.0067M PO4, without Calcium, 500 mL SH30256.01 10462372 6,09
Magnesium, Phenol Red
HEPES 1M Solution 100 mL SH30237.01 10204932 17,91
Trypsin 2.5% 10X Solution, with 25.0 g Porcine Trypsin (1:250)/L in HBSS, 100 mL SV30037.01 10217723 16,51
without Calcium and Magnesium, without EDTA or Phenol Red
** Please enter the promo code 13007 to get access to the promo prices when you order.
8
Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
ActiPro™ and ActiSM™ Cell Culture Media Transfection Media for CHO and HEK
ActiPro medium is intended for batch, fed-batch, or perfusion TransFx-C™ and TransFx-H™ media were developed through the
processes in a variety of culture formats and scales. ActiPro medium HyClone Metabolic Pathway Design process to provide consistent
is used for achieving high yield of active protein during cultivation performance and maximize process yields in transfection and
of recombinant CHO cells. Using ActiPro with Cell Boost 7a and transient expression of recombinant proteins. It allows for flexible
7b supplements in fed-batch or perfusion cultures can contribute use, from microliter volumes to production scale volumes in
to significantly increased cell growth and protein yield. bioreactors.
ActiSM Adaptation Medium Classical Media
ActiSM allows for the cultivation or adaptation of recombinant A wide range of high quality, sterile, ready to use classical media
CHO cells in small-scale shaker flasks or spinner bottles prior to with and without modifications is available to support your cell
transfer of cells to the ActiPro. ActiSM medium can help simplify culture needs.
and shorten the time-consuming adaptaion to fed-batch culture.
His-tagged Protein Purification – Eukaryotic
Media Feeds and Supplements
Cell Culture
These HyClone Cell Boost™ supplements are used in various
feeding strategies to enhance product titer and protein quality His Mag Sepharose™ excel magnetic beads are designed for
in mAb and recombinant protein productions. small-scale purification and screening of his-tagged proteins
secreted in eukaryotic cell culture.
Ordering information
GE catalogue Fisher Scientific Fisher Scientific
Item description Pack size number number promo price (€)**
Classic Liquid Media
RPMI 1640 Medium, with 25 mM HEPES, with L-Glutamine 500 mL SH30255.01 11521881 10,19
RPMI-1640, without L-Glutamine 500 mL SH30096.01 11531851 7,11
DMEM with High Glucose, with 4.0 mM L-Glutamine, with Sodium Pyruvate 500 mL SH30243.01 10749864 7,11
DMEM with High Glucose, with 4.0 mM L-Glutamine, without Sodium Pyruvate 500 mL SH30022.01 10103542 7,11
DMEM/F12 1:1, with 2.50 mM L-Glutamine, 15 mM HEPES 500 mL SH30023.01 10770245 10,19
MEM with Earle's Balanced Salt Solution (EBSS), with 2.0 mM L-Glutamine 500 mL SH30024.01 11591801 7,11
L-Glutamine, 200 mM 100 mL SH30034.01 10453332 9,50
Proprietary Liquid Media
HyCryo 2x Cryopreservation Media 100 mL SR30001.02 13493809 100,39
HyCell™ TransFx-C Liquid Media 100 mL SH30941.02 15220506 41,51
HyCell TransFx-H Liquid Media 1000 mL SH30939.02 15280496 41,62
SFM4CHO with L-Glutamine, with 2.2 g/L Sodium Bicarbonate 1000 mL SH30549.02 10483522 31,07
SFM4CHO without L-Glutamine, with 2.2 g/L Sodium Bicarbonate 1000 mM SH30548.02 10376852 31,36
ActiPro Liquid Medium 500 mL SH31039.01 15835261 30,38
ActiSM Liquid Medium 500 mL SH31040.01 15825261 27,55
Cell Boost 7a Powder Supplement 1L SH31026.07 15805261 112,06
Cell Boost 7b Powder Supplement 0.5 L SH31027.08 15815261 30,23
Cell Boost 2 100 g SH30596.01 11566291 96,97
Cell Boost 5 100 g SH30865.01 11566341 151,54
Cell Boost 6 100 g SH30866.01 11576341 127,68
Sample Prep
His Mag Sepharose excel (HisTag Protein Purification-Eukaryotic Cell Culture) 2 × 1 mL 17-3712-20 12691206 106,54
** Please enter the promo code 13007 to get access to the promo prices when you order.
9
Focus on Fisherbrand™ for your Cell Culture Needs
This section features a wide variety of Fisherbrand consumables and small equipment designed to supplement your everyday cell
culture needs in the laboratory. These items range from water baths, bottle rollers and rockers through to our comprehensive
range of liquid handling and safety products.
Fisherbrand for Cell Culture
Water Baths, Shaking, Isotemp Open Air Rocker
• Intuitive digital control and easy to use • Used in a variety of environmental conditions
• Easy cleaning and maintenance with coil-free design • The electronic tilt adjustment alters the rocking angle while
• Optimise scheduling with auto-on and auto-off timers the unit is operating. Precise speed control provides smooth,
• Help prevent bath overheating and damage with low-fluid low-speed rocking motion down to 1 rpm
protection • Touchpad controls have easy-to-read symbols for speed, tilt
• Conveniently save commonly used settings with four angle, and time. Unit provides repeatable and accurate results.
temperature and shaking speed presets Timer will display elapsed time or, when programmed, will
• Help protect your work with audible alarms shut off unit when time reaches zero
• Easily operate and monitor with icon-based graphical display • Audible and visual signals will activate when system detects
• Features next-generation shaker motor an obstruction or overload of the tray
• Baths come with stainless steel gable cover and shaking tray • Slowly increases speed to desired set-point to avoid splashing
• Operating conditions: usable in refrigerators, cold rooms or non-
CO2 incubators, maximum 80% relative humidity, non-condensing
Aspiration Advantage System • Applications include: staining and destaining gels, hybridisations,
• Superior chemical resistant diaphragm pump with long service life haematology and blotting techniques
• Automatic flow-stop when four litre receiver is full
• Hydrophobic filter for bacteria and viruses, diameter 55 mm,
minimum pore size 0.22 μm Bottle Roller, Digital, CO2 and Humidity Resistant
• Rapid action coupling hose connectors with automatic • Anti-corrosive and wear resistant construction enables use in
isolation valves humid and CO2 environments
• Pumping speed 15 L/min • Stacking kit allows you to stack units two high, saving valuable
• Ultimate pressure 100 mbar bench space
• Tubing required DN 8 mm • Optional remote control facilitates operating unit while in
incubator and adds timer function
• Includes ‘O’ rings for smooth rolling of 15 mL or 50 mL tubes
• Applications include: cell culture, hybridisation assays, liquid
mixing and bioproduction
Ordering information
Fisher Scientific Fisher Scientific
Item description Pack size number price (€)
Fisherbrand Equipment
Isotemp Shaking Water Baths 15 L 1 15325887 1.665,00
Isotemp Shaking Water Baths 27 L 1 15335887 2.100,00
Aspiration Basic System, 15 L/min, 150 mbar 1 15567479 1.130,92
Aspiration Advantage System, 15 L/min, 150 mbar 1 15348191 2.013,73
Open Air Rocker, 1 to 50 rpm, 100–240V, EU and UK Plug 1 15514070 700,00
Fisherbrand Bottle/Tube Roller, Variable Speed, Digital 1 15376617 1.270,00
10Pipette Filler, Motorised Nitrile Disposable Gloves, Extended Cuff,
• For glass and plastic pipettes from 0.1 mL to 200 mL PPE Category III
• Ergonomic hand grip Choose a glove with exceptional durability and added protection.
• Variable speed with two pipette buttons The Fisherbrand extended cuff nitrile gloves are extra-thick and
• Safety valve fully textured for a superior grip.
• NiMH battery, 2.4V, 700maH • Powder free, latex free
• Recharging indicator LED • Fully textured finish for a superior wet or dry grip
• Ambidextrous and non-sterile with beaded cuff
• Thickness: 0.15 mm (palm), 0.18 mm (finger)
• Color: teal green
• Length 305 mm
• Conforms to EN ISO 374/Type B
• Certified for food contact
Ordering information
Fisher Scientific Fisher Scientific
Item description Pack size number promo price (€)**
Fisherbrand Cell Culture Plastics
L-Shaped Cell Spreaders 100 15625467 9,61
L-Shaped Cell Spreaders 500 15615467 39,73
Fisherbrand Safety
Unisex 65% Polyester/35% Cotton Laboratory Coats, Size S 1 11558102 27,75
Unisex 65% Polyester/35% Cotton Laboratory Coats, Size M 1 11568102 27,62
Unisex 65% Polyester/35% Cotton Laboratory Coats, Size L 1 11578102 27,62
Unisex 65% Polyester/35% Cotton Laboratory Coats, Size XL 1 11588102 27,62
Unisex 65% Polyester/35% Cotton Laboratory Coats, Size 2XL 1 11598102 27,62
Unisex 65% Polyester/35% Cotton Laboratory Coats, Size 3XL 1 11508112 27,62
Extended Cuff Nitrile Gloves XS 50 15612367 9,67
Extended Cuff Nitrile Gloves S 50 15662357 8,72
Extended Cuff Nitrile Gloves M 50 15672357 8,72
Extended Cuff Nitrile Gloves L 50 15682357 8,72
Extended Cuff Nitrile Gloves XL 50 15692357 8,72
Fisherbrand Liquid Handling
Motorized Pipet Controller 1 15249805 181,60
Serological Pipet, PS, 5 mL, Magnifier Stripe, Blue Bulk Packed, Sterile, 50/bag 200 11829660 30,68
Serological Pipet, PS, 10 mL, Magnifier Stripe, Orange, Individually Wrapped, Sterile 200 11839660 33,16
Serological Pipet, PS, 25 mL, Magnifier Stripe, Red, Individually Wrapped, Sterile, 50/bag 200 11517752 70,08
Serological Pipet, PS, 5 mL, Magnifier Stripe, Blue, Ind. Paper/Plastic Wrapped, Sterile, 50/bag 200 11869181 30,16
Serological Pipet, PS, 10 mL, Magnifier Stripe, Ind. Paper/Plastic Wrapped, Sterile, 50/bag 200 11879181 37,08
Serological Pipet, PS, 25 mL, Magnifier Stripe, Ind. Paper/Plastic Wrapped, Sterile, 50/bag 200 11839181 71,92
Serological Pipet, PS, 5 mL, No Stripe, Blue, Ind. Paper/Plastic Wrapped, Sterile, 50/bag 500 11899660 66,36
Serological Pipet, PS, 10 mL, No Stripe, Orange, Ind. Paper/Plastic Wrapped, Sterile, 50/bag 500 11809670 80,08
Serological Pipet, PS, 50 mL, No Stripe, Purple, Ind. Paper/Plastic Wrapped, Sterile, 50/bag 100 11829670 108,54
** Please enter the promo code 13007 to get access to the promo prices when you order.
11New lab start up programme Visit the following link to find out more about the GE product offering in the Fisher new lab start up programme: www.eu.fishersci.com/go/nlsu 12
Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
Sample Preparation Savings
up to
40%
Layers 1 and 2
Glass microfiber
prefilter
Layers 1 and 2
Layer 3 Glass microfiber
prefilter
Glass microfiber
prefilter
Layer 4
Membrane
filter Layer 3
Glass microfiber
prefilter
Layer 4
Membrane
filter
Membrane Filters
• Tested and certified for the absence of UV-absorbing
Regenerated Cellulose Membranes substances at wavelengths of 210 and 254 nm with water,
Whatman™ regenerated cellulose membrane filters are microporous methanol, and acetonitrile
cellulose acetate filters made without wetting agents. • Documented batch-to-batch quality and consistency,
ensuring reproducible result
• Chemical resistance suitable for a wide range of aqueous and
organic media Whatman GD/X™ Syringe Filters
• Low extractable levels to minimize sample contamination
These filters are specifically designed for filtration of viscous or
• Mechanically stable with low protein binding
otherwise hard-to-filter samples with high solids content.
• Spontaneous wetting with very good wet strength
• High loading capacity for samples with high solids content
• Three layer glass fiber prefiltration stack for filtering larger
Syringe Filters sample volumes with less back pressure build-up
• Process three to seven times more sample volume than filters
SPARTAN™ HPLC Syringe Filters without prefilter
These filters offer reproducible filtration for ultra-high performance
liquid chromatography (UHPLC) and high performance liquid Puradisc FP Syringe Filters
chromatography (HPLC) sample preparation. SPARTAN filters can The FP 30 filter range contains a low protein binding cellulose
also be used in capillary electrophoresis, protein filtration, UV/VIS acetate membrane, for sterile filtration of cell, tissue culture media
analysis, and any application requiring a chemically resistant, low and samples containing proteins. Available in different pore sizes.
protein binding, hydrophilic membrane in a polypropylene housing.
• Hydrophilic regenerated cellulose membrane exhibits low
protein binding Click here for GE Whatman Filtration for HPLC
• Excellent chemical resistance to common aqueous and Sample Preparation
organic solvents
www.fishersci.co.uk/gb/en/promotions/ge-whatman-filtration-hplc-sample-preparation.html
Ordering information
GE catalogue Fisher Scientific Fisher Scientific
Item description Pack size number number promo price (€)**
Membrane Filters
RC55 Membrane Circles, Regenerated Cellulose, 0.45 µm, 47 mm 100/pk 10-4102-12 11365394 143,40
RC55 Membrane Circles, Regenerated Cellulose, 0.45 µm, 50 mm 100/pk 10-4102-14 10166770 145,80
Syringe Filters
SPARTAN HPLC Syringe Filter, 30 mm, Regenerated Cellulose, 0.2 µm 100/pk 10-4630-60 11369344 141,00
SPARTAN HPLC Syringe Filter, 30 mm, Regenerated Cellulose, 0.45 µm 100/pk 10-4630-50 10589241 139,20
SPARTAN HPLC Syringe Filter, 13 mm, Regenerated Cellulose, 0.2 µm 100/pk 10-4631-00 10701991 122,40
SPARTAN HPLC Syringe Filter, 13 mm, Regenerated Cellulose, 0.45 µm 100/pk 10-4631-10 11309354 122,40
GD/X Syringe Filter, 25 mm, Regenerated Cellulose, 0.2 µm 150/pk 6887-2502 12336603 270,60
GD/X Syringe Filter, 25 mm, Regenerated Cellulose, 0.45 µm 150/pk 6882-2504 10706279 301,20
Puradisc FP Syringe Filter, 30 mm, Cellulose Acetate, 0.45 µm, Sterile 50/pk 10-4621-00 10363051 85,91
Puradisc FP Syringe Filter, 30 mm, Cellulose Acetate, 0.2 µm, Sterile 50/pk 10-4622-00 11399304 85,91
** Please enter the promo code 13007 to get access to the promo prices when you order.
13Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
Syringeless Filters Thimbles
Mini-UniPrep™ Syringeless HPLC Filters Cellulose Thimbles
Whatman Mini-UniPrep Syringeless Filters integrate an autosampler Whatman cellulose thimbles are manufactured from high-
vial, filtration membrane, plunger, and cap/septa into one consumable quality alpha cellulose cotton linter for high-performance
product. They are built for fast and easy HPLC/UHPLC sample use in automated Soxhlet extractor systems. Used in air and
preparation. waste gas analysis.
• Compatible with most major autosamplers for high- • Used in a wide variety of solid-liquid extraction studies,
throughput analysis including lipid and pesticide analyses in food and soil samples
• All-in-one filtration device for quick and cost-effective • Wall thickness of standard thimbles is 1–1.5 mm to support
sample processing application need
• Suitable for temperatures up to 120°C (248°F)
• Excellent mechanical strength and retention
Glass Microfiber Filters
GF/A Glass Microfiber Filters
Whatman Grade GF/A glass microfiber filters feature fine retention
and are suitable for water quality parameter measurement and Click here to find the right filter for your research.
air filtration applications. They have a nominal particle retention
of 1.6 µm.
www.fishersci.co.uk/gb/en/brands/IL10A63H/ge-healthcarehtml#genericTabContent3
Ordering information
GE catalogue Fisher Scientific Fisher Scientific
Item description Pack size number number promo price (€)**
Syringeless Filters
Mini-UniPrep Syringeless Filter, Regenerated Cellulose, 0.2 µm 100/pk UN203NPERC 11308754 142,80
Mini-UniPrep Syringeless Filter, Regenerated Cellulose, 0.45 µm 100/pk UN203NPURC 11305124 142,80
Glass Microfiber Filters
GF/A Glass Circles, 47 mm 100/pk 1820-047 11969144 22,93
GF/A Glass Circles, 55 mm 100/pk 1820-055 11390554 22,93
Thimbles
Grade 603 Cellulose Thimbles, 33 × 80 mm – Thickness 1.5 mm 25/pk 10-3502-40 11310824 45,88
Grade 603 Cellulose Thimbles, 33 × 94 mm – Thickness 1.5 mm 25/pk 10-3502-42 11333085 54,69
Cellulose Thimbles, 33 × 100 mm – Thickness 1.0 mm 25/pk 2800-330 11390704 51,49
Cellulose Thimbles, 33 × 80 mm – Thickness 1.0 mm 25/pk 2800-338 11310714 53,90
** Please enter the promo code 13007 to get access to the promo prices when you order.
14Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
Lab Essentials
Benchkote™ Surface Protector Vacu-Guard 50 mm Disc Filter
Whatman Benchkote is an absorbent lab paper that protects The Vacu-Guard filter is designed for in-line use with stepped
surfaces against hazardous spills. After use, the bench protector barb connections for 10 to 12 mm i.d. hose.
sheet is incinerated or disposed of according to local regulations. • Excellent for protecting vacuum pump systems from solvent
vapor or gaseous contaminants and aqueous aerosols
Grade 105 Lens Cleaning Tissue • Available with choice of chemical trap: activated carbon,
This high-quality lens cleaning tissue is chemically pure and free molecular sieve, or desiccant
from silicones and other additives. • Membrane retains 99.99% of airbone particles greater than 0.1 µm
• Features hydrophobic polytetrafluoroethylene (PTFE) membrane
Kjeldahl Analysis Weighing Boat
Hepa-Vent 50 mm Disc Filter
This weighing boat is designed so users can collect, transfer, and
drop both sample and weighing boat into an acid solution during Hepa-Vent high-efficiency particulate arrestance (HEPA) air
a Kjeldahl analysis without influencing analytical results. filters are used in industrial, research, and scientific applications
that require extremely high retention of airborne particles.
• No glue or chemical additives
• Excellent for both weighing and quantitative sample transfer • Meets high-efficiency particulate arrestance standard,
retaining 99.97% of all particles ≥ 0.3 µm in air
• Depth design results in extremely high retention and
1 Chr Chromatography Paper
bidirectional flow rate
Whatman cellulose chromatography papers are manufactured
from the highest quality cotton linters with no additives.
The papers are manufactured and tested specifically for
chromatographic techniques and undergo rigorous quality control
to ensure uniformity. 1 Chr has a smooth surface, 0.18 mm thick
with a linear flow rate (water) of 130 mm/30 min. These papers
offer good resolution for general analytical separations.
Ordering information
GE catalogue Fisher Scientific Fisher Scientific
Item description Pack size number number promo price (€)**
Lab Essentials
Benchkote Surface Protector, Sheets, 460 × 570 mm 50/pk 2300-916 11300674 55,24
Grade 105 Lens Cleaning Tissue, 100 × 150 mm, 25 Wallets of 25 Sheets 25 × 25/pk 2105-841 11360654 91,67
Kjeldahl Analysis Weighing Boat, Grade 609 100/pk 10-3130-32 11393115 54,41
Grade 1 Chr Cellulose Chromatography Paper, 200 × 200 mm 100/pk 3001-861 11390724 40,32
Grade 1 Chr Cellulose Chromatography Paper, 460 × 570 mm 100/pk 3001-917 11310734 136,80
Vacu-Guard 50 mm Disc Filter 10/pk 6722-5000 11364634 94,80
Hepa-Vent 50 mm Disc Filter 10/pk 6723-5000 11374634 55,25
** Please enter the promo code 13007 to get access to the promo prices when you order.
15Focus on Fisherbrand™ for your Sample Preparation Needs
Our Fisherbrand balances and our new range of water purification systems, which provide Type 1 water suitable for your sample
preparation applications, are complemented in this section by a selection of Fisher chemicals and safety disposal gloves.
Fisherbrand for Sample Preparation
Water Systems, Ultrapure, ACCU20 Balances, Analytical Series
• Produce ultrapure water or RO water on demand from tap • Weighing modes include weighing, parts counting and
water directly with no additional pretreatment filters percentage weighing
• Equipped with a safeguard ACCUDuo ‘P’ pack cartridge and a • Large draft shield with sliding top and side doors
dual-column ACCUDuo ‘U’ pack ultrapurification cartridge to • Dimensions (w x d x h) 196 × 320 × 287 mm
ensure optimum quality of the final product water • Linearity: 0.2 mg
• No external storage tank is required, removing the risk • Repeatabilty: 0.1 mg
of water degradation by contaminants from the container • Communication interface: RS232
or tubing
• Contains automatic cleaning cycles and self-maintenance
functions for the RO membrane, increasing the lifetime and
Vortex Mixers, Classic and Wizard™
efficiency of the RO membranes as well as the cartridges • Innovative and attractive ergonomic design for outstanding
• Automatic hourly system recirculation in the loop prevents comfort
bacterial growth and maintains water purity at all times • Epoxy-coated zinc alloy construction for durability and
• System status and key quality parameters are clearly displayed excellent chemical resistance
on large LCD screen for easy system performance monitoring • Anti-slip feet help to absorb vibration and increase stability
• Wizard model features novel infrared tube sensing system
• Protection: IP42
• Speed 0 to 3000 rpm
• Orbit diameter 4.5 mm
• Warranty: One year
Ordering information
Fisher Scientific Fisher Scientific
Item description Pack size number price (€)
Fisherbrand Equipment
ACCU20 Ultrapure Water System 1 15489689 4.255,00
ACCU20 UV Ultrapure Water System 1 15499689 5.180,00
Analytical Balance 65 g, Readability 0.1 mg, Internal Calibration 1 15395103 1.110,00
Analytical Balance 210 g, Readability 0.1 mg, External Calibration 1 15305113 1.066,00
Analytical Balance, 210 g, Readability 0.1 mg, Internal Calibration 1 15315113 1.254,00
Classic Vortex Mixer 1 11726744 180,00
Wizard Infrared Vortex Mixer 1 11746744 215,00
16Gloves, Latex, Disposable, PPE, Category III Weighing Boats, Polystyrene
• Latex examination gloves providing a protective barrier • Flexible polystyrene boats feature smooth surfaces to provide
• Contain soothing Aloe vera to help prevent skin from drying, accurate pour-outs with minimal sample loss
cracking and chapping • Select white or black for best color contrast to ensure sample
• Textured to provide excellent grip is completely removed
• Powder free • Biologically inert and resistant to dilute and weak acids, aqueous
• To EN 374:2003 (AQL 1.5) solutions, alcohols and bases. Temperature range -10°C to 70°C
• Length 240 mm • Can be used with microwave moisture analysers
• All are FDA/food-grade compliant
• Available in regular or anti-static material formulation
• Disposable
Ordering information
Fisher Scientific Fisher Scientific
Item description Pack size number promo price (€)**
Fisher Chemicals
Ethyl Acetate, for HPLC E/0906/15 Glass 1L 10724181 35,56
Ethyl Acetate, for HPLC E/0906/17 Glass 2.5 L 10456870 62,88
Isopropanol, for HPLC P/7507/15 Glass 1L 10284250 36,24
Isopropanol, for HPLC P/7507/17 Glass 2.5 L 10674732 74,00
Isopropanol, HPLC for Gradient Analysis P/7508/17 Glass 2.5 L 10561802 53,60
Methanol, for HPLC M/4056/15 Glass 1L 10365710 23,80
Methanol, for HPLC M/4056/17 Glass 2.5 L 10675112 39,76
Water, HPLC for Gradient Analysis W/0106/15 Glass 1L 10367171 14,96
Water, HPLC for Gradient Analysis W/0106/17 Glass 2.5 L 10449380 30,08
Acetonitrile for LC-MS Optima™ 2.5 L 10001334 252,24
Fisherbrand Weighing Boats
Weighing Boats, Polystyrene, Anti-Static Black, Diamond, Capacity 5 mL 500 12912860 10,53
Weighing Boats, Polystyrene, Anti-Static Black, Diamond Capacity 30 mL 500 12922860 19,17
Weighing Boats, Polystyrene, Anti-Static Black, Diamond Capacity 100 mL 500 12932860 35,49
Weighing Boats, Polystyrene, Anti-Static Black, Square Capacity 10 mL 500 12942860 8,24
Weighing Boats, Polystyrene, Anti-Static Black, Square Capacity 85 mL 500 12952860 39,51
Weighing Boats, Polystyrene, Anti-Static Black, Square Capacity 280 mL 500 12962860 41,22
Fisherbrand Safety
Latex Gloves with Aloe Vera S 100 15153269 20,72
Latex Gloves with Aloe Vera L 100 15163269 20,72
Latex Gloves with Aloe Vera M 100 15173269 20,72
Latex Gloves with Aloe Vera XL 100 15183269 20,72
** Please enter the promo code 13007 to get access to the promo prices when you order.
17Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
Protein purification information base
Experience the protein purification community
Discussion forum – share insights and ask questions related to protein purification
Knowledge library – including downloads, videos and other resources
Products for proteins – browse tools for protein purification including ÄKTA systems
and prepacked columns
Protein blog and news – keep up to date with latest news and events
ÄKTA™ club – access to information about your ÄKTA system has never been so easy
proteins.gelifesciences.com
GE Purify App
Need further guidance? Protein purification made easy: The Purify app
simplifies the purification process by helping you to quickly select the
right product(s) for your needs.
Download the free Purify App at gelifesciences.com/purify
18Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
ÄKTA™ start
An easy-to-learn and easy-to-use system to remove the hassles
of manual protein purification
Purify tagged proteins and antibodies easily
Gain insight from real-time monitoring
Evaluate and share your results
User friendly – Easy-to-use touchscreen display allows you to start the run at the touch of a button
Convenient – Easy transition from manual to automatic purification
Gain deeper insights – Gain valuable insights from real-time monitoring and control software
Simplify your workflow – Purify tagged proteins and antibodies easily using prepacked column
Need syringe filters for your sample preparation?
See page 13 and 14 for selected Whatman range.
Ordering information
GE catalogue Fisher Scientific Fisher Scientific
Item description Pack size number number promo price (€)**
ÄKTA start
AKTA start – Main Instrument 1 system 29-0220-94 15269764 6.497,00
UNICORN™ start 1.1 1 item 29-2769-64 15873461 840,58
Frac 30 1 item 29-0230-51 15279764 1.274,00
** Please enter the promo code 13007 to get access to the promo prices when you order.
19Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
Purification Savings
up to
20%
Affinity Chromatography
HisTrap™ excel HisTrap HP
The HisTrap excel is a ready-to-use immobilized metal ion affinity The HisTrap HP is prepacked with Ni Sepharose High Performance
chromatography (IMAC) column prepacked with Ni Sepharose excel. and designed for simple, high-resolution purification of his-tagged
Designed for capture and purification of histidine-tagged proteins proteins by IMAC.
from various sample types, including E. coli lysates. Nickel ions are
• High binding capacity, at least 40 mg/mL chromatography resin
very strongly bound to the resin, making it especially suitable for
• Compatible with a wide range of reducing agents, detergents,
purification of his-tagged proteins secreted into eukaryotic cell
denaturants, and other additives
culture supernatants or for samples causing nickel stripping.
• Simple manual operation with a syringe, pump, or
• Increase target protein yield and decrease degradation through chromatography system such as ÄKTA
reduced and simplified sample handling
• Resistant to EDTA (100 mM) and reducing agents such as HisTrap FF crude
DTT (5 mM)
HisTrap FF Crude columns are high binding capacity IMAC columns
for his-tagged protein purification when scalability is needed.
HiTrap™ TALON® crude
• Excellent flow properties – great performance even at high
The HiTrap TALON crude allows for rapid separations and easy
flow rates
scale-up with a minimum of sample preparation and equipment.
• HiTrap column format – compatible with syringe, pump, or
• Suitable for IMAC purification of his-tagged proteins when chromatography system such as ÄKTA systems or other
Ni2+ is not the optimal choice of metal ion FPLC systems
• Compatible with commonly used IMAC reagents and appropriate • High binding capacity – binds up to 40 mg his-tag protein/mL resin
for purifying proteins under native or denaturing conditions • Optimized for unclarified samples – short purification time can
minimize potential negative effects, such as degradation and
oxidation of proteins
• His-tag protein purification from unclarified lysates
A suitable and high dynamic binding capacity will help to save costs. Make sure the resin and column
you choose match to the needed expressed target protein. HiTrap columns can be connected easily in
series up-to three to increase capacity.
Ordering information
GE catalogue Fisher Scientific Fisher Scientific
Item description Pack size number number promo price (€)**
Affinity Chromatography
HisTrap excel 5 × 1 mL 5 columns/pk 17-3712-05 12632966 180,89
HiTrap TALON crude, 5 × 1 mL 5 columns/pk 28-9537-66 11618893 147,26
HisTrap HP 5 × 1 mL 5 columns/pk 17-5247-01 10431065 167,26
HisTrap FF crude 5 × 1 mL 5 columns/pk 11-0004-58 10685355 147,26
** Please enter the promo code 13007 to get access to the promo prices when you order.
20Isolation and
Cell Culture Sample Prep Purification Analysis
Amplification
Ni Sepharose excel His GraviTrap TALON
Ni Sepharose excel is a his-tagged protein purification resin for The His GraviTrap TALON is a prepacked gravity flow column for
minimized Ni-leakage and maximized protein recovery when convenient manual purification of his-tagged proteins.
samples contain stripping agents. • Offers a different selectivity compared to nickel-charged resins
• Versatile – suitable for eukaryotic samples, including insect • One run takes about 30 min, and large sample volumes can be
and CHO cells, as well as bacterial samples such as E. coli applied all at once
• Retained binding capacity of up to 10 mg/mL resin • Convenient his-tagged purification with high purity (> 90%)
• Flexible formats – prepacked and loose resin formats for and binding capacity of up to 15 mg/column
optimized screening and preparative his-tag purification
• Simplified sample handling – fewer steps needed compared His SpinTrap™
with conventional workflow This is a single use, prepacked spin column for purifying his-tagged
• EDTA-resistant resin for IMAC chromatography proteins by immobilized metal ion affinity chromatography (IMAC).
His GraviTrap™ • Allows direct purification of both clarified and unclarified cell lysates
• Yields up to 750 µg pure protein per column
The His GraviTrap is a prepacked, single-use column for • For simple, small-scale purification of his-tagged proteins and
purification of his-tagged proteins by immobilized metal affinity rapid expression screening
chromatography (IMAC). This column gives fast and simple
gravity-flow purifications without any need for a purification.
His SpinTrap TALON
• Convenient, prepacked gravity-flow column
• Allows direct purification of both clarified and unclarified cell lysates The His SpinTrap TALON is a single-use column for rapid screening
• High binding capacity; approximately 40 mg of pure protein with small samples of his-tagged proteins.
per column • Suitable for purification of multiple samples in parallel, such
as screening
• With a microcentrifuge, one run takes about 10 min
• Designed for high purity, small-scale purification and screening
of his-tagged proteins from clarified cell lysates
Ordering information
GE catalogue Fisher Scientific Fisher Scientific
Item description Pack size number number promo price (€)**
Affinity Chromatography
Ni Sepharose excel 100 mL 1 bottle 17-3712-02 12664557 1.271,69
Ni Sepharose excel 25 mL 1 bottle 17-3712-01 13415187 347,24
His GraviTrap 10 columns/pk 11-0033-99 11564865 205,18
His GraviTrap TALON 10 columns/pk 29-0005-94 12664506 169,12
His SpinTrap 50 columns/pk 28-4013-53 11733319 267,45
His SpinTrap TALON 50 columns/pk 29-0005-93 12654506 252,90
** Please enter the promo code 13007 to get access to the promo prices when you order.
21You can also read
