Insulin Resistance in HIV Infection: Drugs, Host Responses, or Restoration to Health?

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Insulin Resistance in HIV Infection        Volume 16 Issue 2 June/July 2008

Perspective
Insulin Resistance in HIV Infection: Drugs, Host Responses,
or Restoration to Health?
Protease inhibitors (PIs) are widely assumed to be associated with a syn-                      are rarely used because of the need to
drome of insulin resistance accompanied by hyperlipidemia and fat redis-                       perform insulin clamp studies to make
tribution. Insulin resistance in HIV infection has numerous other causes,                      the diagnosis (Kahn et al, Diabetes Care,
however, which include not only the direct effects of antiretroviral drugs                     2005). Epidemiologically, insulin resis-
but also factors such as aging and restoration to health accompanied by fat                    tance is strongly linked with coronary
accumulation. Studies of PIs in HIV-infected and noninfected patients indi-                    artery disease, and debate exists over
cate that some of these drugs are associated with reduced insulin sensitivity                  whether it is the prime causal factor or
(greater acute versus chronic effects) that may be due to direct blockade of                   a risk marker. Factors known to cause
the insulin-sensitive glucose transporter in muscle and fat cells. Other studies               insulin resistance outside the setting of
have shown that insulin levels increase over time with antiretroviral therapy,                 HIV infection include obesity, especially
likely the result of improved health, fat accumulation, and aging, and that                    visceral obesity; physical inactivity; the
increases in visceral fat and upper trunk fat are associated with a higher                     use of some drugs (eg, glucocorticoids
risk of insulin resistance in HIV-infected and -uninfected individuals alike.                  and niacin); and acute bacterial infec-
This article summarizes a presentation on insulin resistance in HIV infection                  tion. Keep in mind, however, that ap-
made by Carl Grunfeld, MD, PhD, at the 10th Annual Ryan White HIV/AIDS                         proximately 20% of healthy, thin indi-
Program Clinical Update in Phoenix in June 2007. The original presentation                     viduals have insulin resistance.
is available as a Webcast at www.iasusa.org.
                                                                                               Insulin Resistance in HIV
                                                                                               Infection: Effects of Protease
Protease inhibitors (PIs) are widely as-      (HOMA-IR), which is calculated as fol-
                                                                                               Inhibitors
sumed to induce a syndrome of insulin         lows: insulin concentration (µU/mL)
resistance accompanied by hyperlip-           multiplied by the result of glucose con-         Studies of acute HIV infection usually
idemia and fat redistribution that re-        centration (mmol/L) divided by 22.5; a           show insulin resistance and hyperglyce-
sembles the metabolic syndrome with           score of higher than 4 indicates insulin         mia (with hypoglycemia appearing dur-
its increased risk of cardiovascular dis-     resistance, which is useful in epide-            ing sepsis). Early studies of the insulin
ease. Researchers now believe, how-           miologic studies. Note, however, that            profile in HIV infection (Hommes et al,
ever, that numerous factors contribute        the calculation depends on measuring             Metabolism, 1991) were remarkable in
to the metabolic alterations observed         insulin level and therefore does not             that no insulin resistance or hypergly-
in HIV-infected patients and that some        work using clinical laboratory measure-          cemia was observed; in fact, patients
of these factors may be overlooked if         ments. Similar calculations can be de-           were often thin, and clamp studies in-
only a single cause is assumed.               rived from results of glucose tolerance          dicated increased insulin sensitivity. In
                                              testing. The current gold standards are          2000, Mulligan and colleagues report-
                                              the insulin tolerance test and the eugly-        ed findings in a study that assessed
Insulin Resistance
                                              cemic-insulin clamp technique, the lat-          changes in glucose and insulin before
Insulin resistance exists, but it cannot be   ter of which measures insulin-mediated           and approximately 3 months after the
diagnosed accurately in the clinic. Clini-    glucose disposal (glucose infusion rate          addition of a PI (indinavir, saquinavir,
cal insulin assays are, in short, terrible    divided by insulin level, or M/I). Neither       or ritonavir) or lamivudine to stable
for providing absolute measurements           is appropriate for use in the clinic. The        antiretroviral therapy and compared
of insulin; these assays were developed       bottom line is that insulin resistance is        those results to those in patients main-
as a clinical tool to detect inappropriate    inferred rather than accurately mea-             tained on a stable regimen that did not
insulin levels during hypoglycemia by         sured in a given patient.                        include a PI or lamivudine (control). As
assessing whether insulin levels change          A diagnosis of insulin resistance             shown in Figure 1, patients receiving a
during fasting. (Research insulin assays      portends an unfavorable outcome. In-             PI had a clinically trivial but nonethe-
may work better.) Many studies assess         sulin resistance is the first step toward        less remarkable increase in glucose
insulin resistance using a homeostasis        diabetes, but it must be accompanied             concentrations over the short study
model assessment of insulin resistance        by a (largely genetically determined)            period, as well as a doubling in insu-
                                              reduction in insulin secretion. Insulin          lin concentrations; also, no change in
                                              resistance is one of the World Health            body fat distribution (limb fat and trunk
Dr Grunfeld is Professor of Medicine at the   Organization (WHO) criteria for meta-            fat) was evident (Mulligan et al, JAIDS,
University of California San Francisco.       bolic syndrome, but the WHO criteria             2000). These findings indicate that

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International AIDS Society–USA                               Topics in HIV Medicine

                                                                                                                                                                  P = .009
                                                   Pretherapy        related to restoration of health or a di-
                                                   On therapy                                                                              22
                                                                     rect result of the PI. These studies again

                                                                                                                   M/I (mg/kg*min/µU/mL)
                                                                                                                                           20
                   100        *                                      showed no alteration in visceral or sub-                              18
Glucose (mg/dL)

                                                                                                                                           16
                   80                                                cutaneous fat with PI administration.                                 14
                   60                                                Indinavir, but not lopinavir/ritonavir,                               12
                   40                                                was associated with statistically signifi-                            10
                                                                                                                                            8
                   20                                                cant increases in fasting glucose and in-                              6
                     0                                               sulin concentrations (Figure 2; Noor et                                4
                                                                                                                                            2
                                                                     al, AIDS, 2001; Lee et al, AIDS, 2004).
                                                                                                                                            0
                              *
                                                                         These findings thus suggest that the                                     Mean       Pretreat- Indinavir   Mean
                   30                                                effects of at least some PIs on metabo-                                     Pretreat-     ment              Indinavir
Insulin (µU/mL)

                                                                                                                                                   ment
                                                                     lism do not depend on antiretroviral
                   20                                                                                                                                             P = .026
                                                                     therapy–related restoration of health
                   10                                                and improved immune response or on                                    200
                                                                     changes in body composition. This is not                              180
                     0                                                                                                                     160

                                                                                                                   Glucose (mg/dL)
                         PI          Lamivudine     Control          to say, however, that metabolic changes                               140
                                                                     related to body composition factors do                                120
                                                                     not also occur. Clamp studies in the pa-                              100
 Figure 1. Changes in plasma glucose and in-                         tients receiving indinavir showed a sta-                               80
 sulin levels in HIV-infected patients after add-                                                                                           60
                                                                     tistically significant decrease in insulin                             40
 ing a protease inhibitor (PI) (indinavir, n = 16;                   sensitivity; results of 2-hour oral glucose                            20
 saquinavir, n = 2; or ritonavir, n = 2) or lami-                    tolerance testing showed a statistically                                0
 vudine (n = 9) to stable treatment containing                                                                                                    Mean       Pretreat- Indinavir   Mean
                                                                     significant increase in glucose levels,                                     Pretreat-     ment              Indinavir
 neither (control, n = 12). Asterisks indicate                       with 1 patient meeting the criterion for                                      ment
 statistically significant difference. Adapted                       diabetes and 2 patients meeting the cri-
 from Mulligan et al, JAIDS, 2000.                                   terion for impaired glucose tolerance            Figure 3. Changes in insulin-mediated glu-
                                                                     (Figure 3). However, the insulin resis-          cose disposal (glucose infusion rate divided
                                               Pretherapy            tance observed in these patients was             by insulin level [MI]) in clamp studies (top),
                                               4-week therapy
Glucose (mmol/L)

                   5.6
                                                                     not classic insulin resistance because           and glucose levels in 2-hour oral glucose
                                  P = .05
                   5.4                                               no resistance to the effects of insulin on       tolerance testing (bottom) in HIV-seronega-
                   5.2                                                                                                tive patients receiving 4 weeks of indinavir.
                     5
                                                                     lipid metabolism was observed. Mea-
                   4.8                                               surement of free fatty acid levels during        Top dashed line indicates diabetes thresh-
                   4.6                                               oral glucose tolerance testing showed            old; bottom dashed line, impaired glucose
                   4.4
                         Indinavir          Lopinavir/ritonavir      that levels were normally suppressed in          tolerance threshold. Adapted from Noor et
                                                                     both indinavir and lopinavir/ritonavir re-       al, AIDS, 2001.
Insulin (pmol/L)

                                                                     cipients (Figure 4); similar findings were
                   150
                   100
                                  P = .04                            made in clamp studies.                           hibited insulin resistance (reduced M/I),
                    50                                                   The findings indicating normal lipid         with the reduction in glucose infusion rate
                     0                                               kinetics in these subjects are of particu-       during the clamp study evident within
                         Indinavir          Lopinavir/ritonavir      lar interest given in vitro findings indi-       30 minutes. Glucose disposal during the
                                                                     cating that PIs bind to and block the            hyperinsulinemic clamp is caused by in-
                                                                     insulin-sensitive glucose transporter            sulin increasing glucose transport by in-
  Figure 2. Plasma glucose and insulin levels                        (GLUT4; Hruz, Am J Infect Dis, 2006).            creasing the activity of GLUT4 in muscle
  before and after 4 weeks of protease inhib-                        Protease inhibitors decreased insulin-           and fat. Studies of other PIs showed that
  itor treatment in healthy HIV-seronegative                         stimulated glucose transport in fat cells        single-dose ritonavir—but not amprena-
  patients. Adapted from Noor et al, AIDS,                           with no blockade of any aspect of in-            vir—reduced M/I (Figure 6), and that 10
  2001, and Lee et al, AIDS, 2004.                                   sulin activation (eg, phosphorylation,           days of lopinavir/ritonavir also reduced
                                                                     lipid metabolism). The direct effect on          M/I. The picture that has emerged from
 whereas PIs do cause some metabolic                                 GLUT4 was supported by showing that              these studies is that the effects caused
 changes, the changes are not mediated                               glucose transport was blocked in non-            by some PIs of increasing insulin resis-
 by alteration of body composition.                                  insulin-sensitive cells transfected with         tance are strong in the short term and
    Dr Grunfeld and colleagues thus per-                             GLUT4. These effects of PIs were evi-            reduced with long-term dosing (Lee et
 formed studies in which indinavir or                                dent within minutes of exposure.                 al, Curr HIV/AIDS Rep, 2000). The great-
 lopinavir/ritonavir were administered                                   Dr Grunfeld and colleagues therefore         est effects are observed with indinavir
 to healthy HIV-seronegative patients to                             examined the effects of single doses of          and full-dose ritonavir, the drugs in use
 determine whether the metabolic ef-                                 PIs in healthy HIV-seronegative patients.        when diabetes began to appear in HIV-
 fects in HIV-infected patients might be                             As shown in Figure 5, every patient ex-          infected patients.

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Insulin Resistance in HIV Infection                                    Volume 16 Issue 2 June/July 2008
Free Fatty Acid Level (mmol/L)

                                 .50                         Pretreatment                that all groups had a statistically sig-                                                                               P < .001
                                                             Indinavir                   nificant increase in insulin levels over                                                      20

                                                                                                                                                              M/I (mg/kg*min/µU/mL)
                                 .40                                                                                                                                                   18
                                                                                         time (Figure 7). Shorter-term follow-
                                                                                                                                                                                       16
                                 .30                                                     up among nonrandomized patients                                                               14
                                                                                         receiving didanosine/stavudine versus                                                         12
                                 .20                                                     abacavir/lamivudine showed a statis-                                                          10
                                                                                                                                                                                        8
                                                                                         tically nonsignificant greater increase
                                 .10                                                                                                                                                    6
                                                                                         in the patients receiving nucleoside                                                           4
                                  0                                                      analogue reverse transcriptase inhibi-                                                         2
                                        0        30     60   90   120   150   180        tors (nRTIs) than in patients receiving                                                        0
                                                  Indinavir (minutes)                                                                                                                             Mean      Placebo   Indinavir     Mean
                                                                                         NNRTIs (nRTIs are themselves associ-                                                                    Placebo                          Indinavir
                                                                                         ated with specific effects on metabo-
                                                                                         lism that are not discussed here; Shlay
                                                                                                                                                                                            14                         Indinavir
                                                             Pretreatment                et al, JAIDS, 2005; Shlay et al, JAIDS,

                                                                                                                                                              Glucose Infusion Rate
                                                                                                                                                                                                                       Placebo

                                                                                                                                                              (mg/kg*min/µU/mL)
                                                                                                                                                                                            12
Free Fatty Acid Level (mmol/L)

                                 .50   P = .04               4-week treatment            2007).
                                                                                                                                                                                            10
                                                                          P = .003
                                                                                            The factors causing increased in-
                                 .40                                                                                                                                                         8                                          P < .05
                                              P = .01
                                                                                         sulin resistance in these treated pa-
                                                                                         tients, which appear to overwhelm the                                                               6
                                 .30
                                                                                         changes produced by PIs, are likely                                                                 4

                                 .20                                                     related to changes such as improved                                                                 2
                                                                                         health and gaining of body fat, as well
                                 .10                                                     as aging. In the Fat Redistribution and                                                                  0   20 40 60 80 100 120 140 160 180 200
                                                                                                                                                                                                                 Minutes
                                  0
                                                                                         Metabolism (FRAM) study (Grunfeld et
                                        0        30     60   90   120   150   180        al, JAIDS, 2007), Dr Grunfeld and col-
                                            Lopinavir/ritonavir (minutes)                leagues assessed metabolic and body                                      Figure 5. Effect of single dose of indinavir in
                                                                                         composition profiles in nondiabetic                                      HIV-seronegative patients on insulin sensi-
                                                                                         HIV-infected patients and healthy con-                                   tivity (M/I; top) and on glucose infusion rate
 Figure 4. Comparison of free fatty acid lev-
                                                                                         trols. Glucose levels were similar in                                    over time during clamp study (bottom).
 els during oral glucose tolerance testing in
                                                                                         male and female patients and con-
 healthy HIV-seronegative patients receiving                                                                                                                      assessed by HOMA-IR values above 4
                                                                                         trols; HOMA-IR levels were somewhat
 4 weeks of indinavir or lopinavir/ritonavir.                                                                                                                     is stronger in controls than in HIV-in-
                                                                                         higher in HIV-infected women, but not
 Adapted from Noor et al, AIDS, 2001, and                                                                                                                         fected subjects because other factors,
                                                                                         in HIV-infected men, compared with
 Lee et al, AIDS, 2004.                                                                                                                                           such as antiretroviral drugs, contribute
                                                                                         controls. Multivariate analysis showed
                                                                                         that being in the upper tertiles for vis-                                to insulin resistance in patients with
                                                                                         ceral fat and upper trunk fat was asso-                                  HIV infection that are not operative in
 Other Factors in Insulin                                                                ciated with a markedly increased risk                                    controls. Factors predictive of greater
 Resistance: Restoration of                                                              of having a HOMA-IR value higher than                                    visceral fat were male sex, white race,
 Health, Fat, and Aging                                                                  4 (Table 1). The relationship between                                    increasing age, not smoking, and re-
                                                                                         fat deposits and insulin resistance as                                   duced physical activity (Table 2).
 The effects of indinavir on insulin re-
 sistance are greater in HIV-infected pa-
 tients than in noninfected patients, in-                                                                                       P = .007                                                                         P = NS
                                                                                                             14                                                                        14
 dicating that factors in addition to the
                                                                                     M/I (mg/kg*min/µU/mL)

                                                                                                                                                               M/I (mg/kg*min/µU/mL)

 potential blocking of insulin-mediated                                                                      12                                                                        12

 glucose transport are operative in HIV                                                                      10                                                                        10
 infection. That such factors are active                                                                      8                                                                         8
 in addition to the specific drug effect
                                                                                                              6                                                                         6
 of some PIs is supported by long-term
 data. In the Flexible Initial Retrovirus                                                                     4                                                                         4
 Suppressive Therapies (FIRST) study,                                                                         2                                                                         2
 patients in whom a PI was added to                                                                           0                                                                         0
 treatment had an early increase in in-                                                                           Average   Placebo   Ritonavir   Average                                        Average    Placebo Amprenavir Average
 sulin levels compared with those add-                                                                            Placebo                         Ritonavir                                      Placebo                      Amprenavir

 ing a nonnucleoside analogue reverse
 transcriptase inhibitor (NNRTI) or PI
 plus NNRTI treatment; however, fol-                                                     Figure 6. Effect of single doses of ritonavir (left) and amprenavir (right) on insulin sensitivity
 low-up of more than 5 years showed                                                      (M/I) in HIV-seronegative patients. NS indicates not statistically significant.

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International AIDS Society–USA                                 Topics in HIV Medicine

                                          Change                                                                                                           Change
                                                       SE       P value                                                                                                  SE     P value
                                        (unit/month)                                                                                                     (unit/month)
                             PI              0.07      0.02
Insulin Resistance in HIV Infection         Volume 16 Issue 2 June/July 2008

Table 2. Effects of Subject Characteristics on Visceral Adipose Tissue in Fat Redistribution        Diabetes Association and the European As-
and Metabolism Study                                                                                sociation for the Study of Diabetes. Diabetes
                                                                                                    Care. 2005;28:2289-2304.
                                         Men                               Women                    Lee GA, Rao MN, Grunfeld C. The effects
                                                                                                    of HIV protease inhibitors on carbohydrate
                            Percent Effect      P Value       Percent Effect       P Value
                                                                                                    and lipid metabolism. Curr HIV/AIDS Rep.
Ethnicity, vs White                                                                                 2005;2:39-50.

 African American                 –55             .001              –21              .089           Lee GA, Seneviratne T, Noor MA, et al. The
                                                                                                    metabolic effects of lopinavir/ritonavir in HIV-
 Hispanic                          –3              .74               +6              .78            negative men. AIDS. 2004;18:641-649.
Age, per decade                   +31
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