FOR THE SAKE OF CLARITY - Draeger
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FOR THE SAKE OF CLARITY Sewage doesn’t disappear that easily. It is purified in up to four stages and then fed back into bodies of water. On the way there, the finest analysis provides detailed information – even about the sociodemographics of the population. TEXT NILS SCHIFFHAUER PHOTOS PATRICK OHLIGSCHLÄGER 50 DRÄGER REVIEW 123 | 1 / 2021
RUBRIK SCIENCE THEMA SEWAGE ALL CLEAR The purified sewage ends up in one of these final clarifiers been using chemical and microbiological analysis to look beyond the supply and disposal of water in order to gain insights ou are what you eat, so they say. into people’s health and their consumption And if you think a few hours further ahead, habits as well as the sociodemographic something else can be added to this saying: structure of entire districts. “... and what you leave behind you.” Urine To this end, the sewage is analyzed and feces end up in the toilet, together using ever finer methods. More recently, with toilet paper and other things that these methods have also helped to trace don’t actually belong there. This mixture the SARS-CoV-2 virus, which caused the is flushed away with water and ends up disease known as Covid-19 (“coronavi- in the wastewater treatment plant via the rus”). Even before the WHO declared the sewer system. Domestic and industrial infection a global pandemic on 11 March wastewater mostly accumulates here. 2020, Professor Gertjan Medema, senior For a number of years, scientists have microbiologist at the KWR Water Research DRÄGER REVIEW 123 | 1 / 2021 51
SCIENCE SEWAGE VIRUSES IN THE SEWAGE INDICATE INFECTIONS – INCLUDING COVID-19 Institute in the Dutch city of Nieuwegein, lutionary water researchers, and Aachen- set to work with his team to prove the pres- based water researchers also showed that ence of the specific residues of the caus- the presence of genetic SARS-CoV-2 mate- al virus in the sewage: “If the number of rial in water treatment plants can also be infected people increases, the viral pollu- proved with modern molecular methods. tion in the sewage should also increase,” In all nine of the water treatment plants says the microbiologist, explaining his tested in April 2020, analyses recorded approach. “As such, corresponding mon- between three and 20 genocopies per itoring of the sewage can ultimately serve milliliter of raw sewage, a concentra- as an early-warning system for the out- tion level also measured in studies in break (or recurrence) of Covid-19.” The the Netherlands and the USA. “If sensi- team pursued a similar concept in 2009. tive enough, such analyses could serve as an early-warning system for the authori- SKIMMING SIGNS IN THE SEWAGE ties,” summarize the authors, “to enable Large screws keep the aquatic plants at bay Back then, the scientists were initially them to identify a local rise in cases in in the clarifying basins looking at the bird flu virus; then they the area around a water treatment plant that obviously feel broadened their interest to include causes at an early stage.” at home here of diseases such as polio, hepatitis A, and The project launched in spring the norovirus, which causes vomiting 2020 under the aegis of Professor and diarrhea. Health officials in the Georg Teutsch from the Helmholtz Netherlands recorded the first Covid-19 Center for Environmental Research in case on February 27, 2020, almost Leipzig (“Integral SARS-CoV-2 Sewage three months after the outbreak of the Monitoring”) essentially wants to bring know, for example, whether the virus epidemic in the Chinese city of Wuhan. clarity to the matter. “By continuously content in the stools changes in one and The microbiologists started taking the monitoring the sewage, we want to the same person as the disease progresses first samples just three weeks before shine a light on the unreported cases, or whether the rate differs from one the country’s first case. Their aim: to provide a basis for taking decisions, person to the next.” These are questions prove the presence of virus-specific and establish an early-warning system,” that make it just as difficult to translate nucleic acids excreted in the stools of says the hydrogeologist. Members of the virus figures from the sewage into those who were infected. The scientists the German Association for Water, the actual number of infected people as traced the geographic progress of the Wastewater, and Waste (DWA) – including it does to establish a clear and reliable infection over time by taking samples at Cologne’s municipal drainage works early-warning system. We currently have specific locations agreed in conjunction (StEB) – are also involved in the project. around 20 people working on clarifying with the health authorities. Professor Many problems have reportedly already this and other issues,” adds Prof. Teutsch. Medema’s study in Amersfoort provided been solved, allowing the scientists to “That’s how science works!” an indication of the potential suitability of identify evaluable signals in the highly the concept as an early-warning system – diluted samples. The main question now FINDING THE SOURCE subject to further research. Traces of the turns to what it all means. “An infected This is also the starting point for the virus were found in the sewage before a individual,” explains Prof. Teutsch, “does supply and disposal of water as we know single case had even been reported to the not excrete viruses on the very first it today. In 1854, the English physician local health authority. day. And the amount of virus excreted John Snow bent over a map of London In Germany, a consortium of Frank- differs for each infected person. Since and marked every cholera fatality with furt-based virologists, ecotoxologists, evo- we measure the total content, we don’t a dash. Then he focused on the water 52 DRÄGER REVIEW 123 | 1 / 2021
“THERE IS A RISE IN THE AMOUNT OF PAINKILLERS” Dr. Andrea Poppe is the head of the StEB’s sewage institute and is a member of the board at the DWA* – it boasts 30 years’ experience in the sampling and analysis of sewage. Dr. Poppe, what is the purpose of performing this analysis? With around 30 employees in our laboratory, we keep the population safe by constantly monitoring the quality of the sewage and ensuring compliance with legal regulations. Even if we are not allowed to conduct research ourselves, because sewage charges directly serve our actual purpose, we are, of course, focused on the future. Here we take part in scientific studies like the Helmholtz Center’s coronavirus project. What has changed over the years in terms of the analysis we know today? It now gives us much more precise and in-depth insights into the substances contained in the sewage. Chemical analysis complements biological methods where changes in the behavior of fish are examined, for example. What things in the sewage are especially problematic nowadays? We are seeing a rise in the amount of pharmaceutical substances, such as diclofenac, which is contained in painkillers. These are freely available to buy and are clearly taken in large quantities prophylactically. They end up in the water via excrement or as a result of not being disposed of properly. Pain relief ointments also contain this substance, which then ends up in the pumps and marked these with a dot. The geographical link between the two was sewage when showering. X-ray contrast agents from doctor’s surgeries or uncanny: The cholera epidemic of 1854 hospitals are equally problematic. And while toilet paper disintegrates in was clearly caused by polluted water at the process, the same cannot be said of moist wipes, which are increasing one of the pumps. This ran counter to being used. They block pipes and pumps and don’t belong in the toilet! the theory popular at the time that this How will the future of analysis develop? disease was caused by miasmas. Yet this It will certainly improve in terms of the quantities and substances it is able to proved to be incorrect. It was a bacterium, detect. The examination of sewage must keep pace with the changes in the which an Italian doctor had discovered in the same year. Even though it would be behavior of our customers. Microplastics, for example, are another challenge. another 30 years before Robert Koch could * The German Association for Water, Wastewater, and Waste prove the hypothesis of microorganisms, John Snow’s discovery had consequences. Particularly in the wake of the sewage scandal of 1858, known as the Great Stink, it led to a fundamental change in the way water was supplied and disposed of in London. “In a certain way, we are still guided by these insights and methods,” says Dr. Andrea Poppe, head of the StEB’s sewage DRÄGER REVIEW 123 | 1 / 2021 53
SCIENCE SEWAGE FROM SEWAGE TO CLEAR WATER WITHIN A DAY 4.5 institute. One of Europe’s biggest and most modern sewage treatment plants runs right along the Rhine in the northeast of the city. The StEB manages its large treatment plant on a site covering 35 hectares. “It is designed for the almost 1.6 million so-called ‘population equivalents,’ which billion people worldwide live we use to refer to the combined capacity for without functioning sewer systems households and industry,” explains Martin Grudzielanek. According to the head of operations, this is where the purification begins for up to 8.2 cubic meters of water per second – from households and industry. That is the equivalent to a total PRECISE CHECK Sewage is constantly of almost 80 million cubic meters per year. 80% being sampled and At the end of a process that takes just 24 analyzed at different hours, during which the main task is to locations absorb bacteria, around 220 million liters of purified water flow into the Rhine from eight final clarifiers up to 70 meters in of the world’s sewage is channeled diameter. into the environment untreated THEY COME UNANNOUNCED has generated itself. The StEB employees For anyone who has stood at the strong- regularly check the contents of the sewage smelling entry point and screening plant, system: “As such, I see places that not which rakes out the coarsest matter before everybody gets to see that easily,” says draining it of water (up to 6,000 tons Tobias Krebs. The chemistry technician per year), the result is a minor miracle. “draws sewage samples,” as they say in the That’s because it is not just fecal matter industry. “Including from companies or that floats along the sewage system to An estimated the airport – wherever the sewage enters 55% Stammheim. It is joined by toilet paper, the system – and of course unannounced.” hygiene articles, food waste, dead rats, and Immediately afterwards while still in the other things. Dräger fixed gas detectors car, the fresh suspension is homogenized (Dräger PIR 3000/7000 and Polytron on a stirrer, divided into smaller vials, 3000) raise the alarm as soon as the gas is the figure by documented, and taken to the laboratory content (such as hydrogen sulfide or which global water consumption in a cool bag for further examination – methane) exceeds certain values. Sand is expected to rise by 2050 including a retained sample so that the and suspended matter are filtered out in original sewage can be used at any time in several stages. The sludge is treated further the event of a need to discuss any matters. ILLUSTRATION: ISTOCK until it harmlessly burns and can be used The Helmholtz researchers also advised for generating energy. From 2030, the Dr. Andrea Poppe’s laboratory on the city of Cologne plans to completely power best places to take samples for proving its treatment plants using electricity it the presence of the virus. “We are also 54 DRÄGER REVIEW 123 | 1 / 2021
WHAT MAKES SOCIETY TICK? A new trend involves using sewage to produce sociodemographic data, which could even dispense with the need for a census or at least complement it. How many residents in a city have a school education? What percentage of them are divorced? Saer Samanipour studies this and much more, not just with demographic statistics, but by analyzing the wastewater. The doctor of chemistry and current professor of molecular science used precise sensory analysis to test the sewage of Australian cities for 40 substances from completely different areas: drugs, antidepressants, sweeteners, or food. He used the concentration of substances such as ethanol and amitriptyline as markers for the consumption of alcohol and antidepressants. He linked his findings with sociodemographic data gathered from a census conducted at the same time. This allowed Samanipour to develop a system of equivalents: a kind of yardstick that he could place alongside the results of the chemical analysis in order to btain data about the population’s social structure. From sewage systems of unknown origin to him, he was able to make sometimes accurate assertions about the social structure of the corresponding district. FATBERGS CLOG SEWER PIPES Time and again, sewer workers find large quantities of fat in the pipe systems during inspections. Over time, they grow into huge lumps. Such deposits in the sewer system occur when fats and oils are disposed of via washbasins or toilets and end up in the sewage. getting involved in this project by sending Objects such as wet wipes, diapers or cotton swabs that do not samples,” says Dr. Poppe. belong in the toilet also cause these lumps. In 2017, one such There is still no absolute certainty “fatberg” blocked London’s sewer system. It was 250 meters long as to whether the coronavirus spreads and weighed more than 100 tons. Every year, Thames Water spends through the sewage. A study published more than 13 million euros alone on clearing such deposits in the in Nature in the summer found that sewer system of the British capital. excreted viruses can remain active for a few days in the wastewater of a sewer Here is a selection of things that do not belong in the toilet: system and in aerosols. It concludes that •Leftover food; kitchen waste a risk assessment should be carried out •Medication; tablets and proposes disinfection with chlorine, •Moist wipes, moist toilet paper treatment with ozone, or processes such as •Hygiene articles like panty liners, condoms, disposable micro- and ultrafiltration with membrane diapers, or sticking plasters filters. Other scientists dismiss this and •Toxic and aggressive substances like some toilet cleaners point to the high dilution level, the short •Deep-frying fat; cooking oils lifespan, and the fact that just a fraction of the virus material actually contains active viruses in any case. Mankind’s sewage disposal has always been put to good use in the past. Analysis and evaluation continue to write the story in unimagined ways. DRÄGER REVIEW 123 | 1 / 2021 55
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