FALCON IITM Wide-Field Hyperspectral Imaging System
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FALCON II TM Wide-Field Hyperspectral Imaging System Unique, Easy-To-Use Software for Automated System Control and Sophisticated Image and Data Analysis Produce Chemical Images that Detail Material Morphology, Composition, Structure and Concentration Modular Configuration with the Ability to Add Other Imaging Modes Such as Near-IR and Fluorescence
Get The FALCON IITM Advantage
The FALCON II is the only Wide-field Raman Chemical Imaging
System optimized for both chemical imaging and spectroscopy.
It employs high throughput Liquid Crystal Tunable Filter (LCTF)
technology and Electron Multiplication Charge Coupled Device
(EMCCD) cameras for Wide-field Hyperspectral Imaging. This
liquid crystal based imaging method enables users to obtain
simultaneous spatial and chemical information. The system is
also equipped with a separate channel for performing standard
dispersive spectroscopic measurements. This combination
of powerful analytical techniques combined with optional
Hyperspectral Near-Infrared (NIR) or Visible/Fluorescence
Imaging are a few examples of how FALCON II can provide
your complete microscopic chemical imaging solution.
What is Raman Chemical Imaging?
Raman Chemical Imaging (RCI) combines digital imaging
and Raman spectroscopy to provide chemical images that
reveal material morphology, composition, structure and
concentration. Chemical Imaging takes advantage of the Features Advantages
user’s natural visual senses and perception to make complex
Liquid Crystal Tunable • Rapid imaging
analysis more intuitive and straightforward.
Filter (LCTF) based • Capture the full field-of-
The FALCON II has diffraction-limited spatial resolution imaging view at once
approaching 0.35 microns when using 100x magnification. • Chemically specific
The spatial resolution of point mapping systems are imaging
typically 1-2 microns and ultimately limited by the laser • High spatial resolution
beam spot size. However, with a FALCON II system • High spectral resolution
every pixel in the chemical image contains an associated • Nondestructive
spectrum for chemical identification. • Little to no sample
preparation
Real time imaging • View dynamic systems
Save Time & Money • Target specific chemical
entities
FALCON II generates spatially and spectrally accurate images
with increased speed compared to point mapping systems. Duet Vision Technology* • Simultaneous
acquisition of spectra
Point mapping systems often oversample spectrally *Available on Professional & and images to identify
and under sample spatially resulting in the collection of Plus Series areas of interest
extraneous information at the cost of measurement time. By
employing liquid crystal tunable filters, FALCON II chemical Automated data • Less user time required
images may be collected only over the spectral regions acquisition • Run experiments
of interest often decreasing data acquisition time without continuously, even over
sacrificing data quality. night
For Raman Chemical Imaging or spectroscopic applications, Option for multiple • Additional chemical
limited or no sample preparation is required. Nondestructive imaging platforms information without
sample characterization can be performed through glass (e.g. Fluorescence or NIR) moving the sample
containers, thin plastic bags or blister packs. In addition,
Raman Chemical Imaging and spectroscopy are compatible Powered by • Rapid data acquisition
with aqueous systems. ChemImage Xpert TM • Powerful data analysis
Software platform
Application Possiblities
The FALCON II has an extensive list of applications and provides
the data necessary for accurate, fast decisions that accelerate
product development and increase your competitive advantage.
Samples Capable of Being Measured with
Little to No Sample Preparation*
• API characterization • Illicit drug substances
• Tablets • Explosive residue
• Emulsions • Semiconductors
• Polymers/solid drug • Photonic materials
solutions • Ceramics/composites
• Polymorphs • Bio/chem threats
• Controlled release beads • Toxic industrial
• Stents chemicals
• Nasal sprays • Toxic industrial
• Molecular probes materials
• Hair/fibers • Glass
• Paint • Corrosion products
• Tape • Interferences
• Inks/dyes/stains
* Some applications require additional method development
Automated & Highly Configurable
The FALCON II is an automated system, including features like: 2D montage imaging; time lapse or dynamic imaging; autofocus;
vibration dampening due to a built-in pneumatic table; and overnight data acquisition capabilities. A full range of custom options
and accessories are offered to meet both standard and more challenging application requirements. The FALCON II can be configured
to operate in the following imaging modes for a complete imaging solution:
Fluorescence NIR VIS
Imaging Measurement Raman
(Optional) (Optional) (Optional)
Ingredient-specific particle sizing X X X X
Content uniformity X X X X
% composition determination X X
Coatings/layer thickness X X X X
Classification/identification X X
Amorphicity, crystallinity, orientation & strain X
CIE L*A*B* color quantification X
FALCON II additional imaging modalities include: brightfield reflectance, brightfield transmission, cross polarization and
differential interference contrast.
Data Analysis Example: Ingredient-Specific (A)
Particle Sizing of a Nasal Spray API
Particle sizing techniques based on laser scattering, cascade
impaction or optical image analysis cannot discriminate
between particles of API and those of an excipient or a
surfactant. Multi-stage cascade impaction followed by
extraction and HPLC analysis is an option but can be extremely
time consuming and labor-intensive and is not reproducible.
Consequently, the development of an efficient ingredient- (B) (C)
specific approach is essential for ensuring that all ingredients ______
fall into the proper size range, particularly during the early 40mm
stage of product development.
The FALCON II system efficiently collects millions of
spectrally unique data points at sub-micron spatial Figure 1. (A) Brightfield Reflectance/Raman fusion image
resolution. The particle size distribution of the API in a nasal illustrating the spatial distribution of the nasal spray API shown
spray suspension is ultimately determined from the chemical in green across the sample (B & C) Enlarged regions of interest
images. A Brightfield Reflectance & Raman fusion image is to emphasize the detection of agglomerated API particles bound
shown in Figure 1 for both a large image montage as well to excipient material
as two individual fields-of-view. Representative Raman
spectra of the API and excipients are shown in Figure 2. Raman Spectra
The histogram in Figure 3 shows the distribution of 123 API
particles calculated by ChemImage XpertTM. The D50 size of
the API particles were 3.9 ± 3.0 µm.
EXCIPIENT #1
Offset Intensity
Software Tools API
EXCIPIENT #2
The FALCON II comes equipped with ChemImage Xpert
EXCIPIENT #3
Software. ChemImage Xpert contains sophisticated spectral
matching and image processing capabilities rivaling commonly EXCIPIENT #4
available spectroscopic and imaging software packages. In 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 3000
addition, this software provides automated hardware control Raman Shift (cm-1)
for overnight experiments or remote access needs.
Figure 2. Raman spectra of the nasal spray API and excipient
With ChemImage Xpert you can selectively detect, analyze components
and identify chemical and biological samples, then visualize Particle Size Distribution
the data on any dimension-of-interest using both traditional 25
image-processing techniques and sophisticated chemometric Total Particles:123
spectral analysis techniques. The combined spatial and 20
D10 = 1.5 mm
spectral information reveals subtle features of a material often D50 = 3.9 mm
Frequency
15 D90 = 8.2 mm
not observable using traditional imaging techniques. Std. Dev. = 3.0 mm
10
Whether you are collecting and analyzing pharmaceuticals,
chemical warfare agents, polymers, air samples, or other 5
substances, ChemImage Xpert will help you understand the
collected materials in terms of what substances are present, 0
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
how much, how pure and where they are found. Max. Chord, mm
Figure 3. Particle size distribution for 123 particles based on
the maximum chord of each particle
Technical Specifications Available Near Infrared Imaging
• Optical resolution
Basic Imaging Specifications (@ 760 nm=1 µm)
• Par-focal among objective selections • NIR spectral range
• Par-centricity ± 10 µm 900 –1700 nm
• Passive environmental vibration damping • Spectral resolution 8 nm
• InGaAs FPA CCD camera
Raman Imaging • Maximum optical
• Optical resolution near diffraction limited to 350 nm magnification 200X
• Spectral resolution 9 cm-1 FWHM (based on Raleigh criteria) • Excitation source – Quartz
• 0.5 cm-1 peak shift discrimination tungsten Halogen, 100W
• Measured Raman range from 244 cm-1 to 5,464 cm-1
shift from laser line Mechanical Specifications
• High sensitivity deep cooled back-illuminated CCD –70°C • Basic dimensions – 92cm
• Optional EMCCD (Professional & Standard Series) deep x 69cm wide x
• Maximum optical magnification 400X 191cm tall
• Excitation source solid-state single frequency, single • Weight ~295 kg (Max.)
mode 532 ± 0.1nm 2-watt laser • Sample stage travel X and
Y = 10 cm x 10 cm
Color Video Imaging • Sample X and Y positioning
• High quality color video resolution – 0.05 µm
• Integration to 8.5 sec. allows use in low light • Sample Z resolution – 0.1 µm
• DSP control for precise, flexible imaging • Sample formats - accepts 25 mm x 75 mm, 50 mm x 75
mm microscope slides, and standard well plate
Available Fluorescence Imaging
Electrical Specifications
• Optical resolution near diffraction limited to 350 nm
• Fluorescence spectral range 400-720 nm • 110 VAC 60Hz
• Spectral bandpass 7 nm
• High sensitivity deep cooled CCD –70°C
• Maximum optical magnification 400X ChemImage Xpert File Compatibility
• Excitation wavelength 365 nm, derived from Hg arc lamp
Image Import (Open)
and excitation filter
• ChemImage Hypercube (.tif)
Raman Dispersive (Professional Series) • Tiff (.tif)
• Dispersive spectral resolution 3 cm-1 FWHM (based on • Perkin Elmer (.fsm)
Raleigh criteria) • Envi (.img)
• Measured Raman range from 244 cm to 4,454 cm shift • Winspec (.spe)
-1 -1
from laser line • User Defined
• Automated three grating turret
Image Export (Save)
• Automated slit width
• High sensitivity deep cooled back-illuminated CCD –70°C • ChemImage Hypercube (.tif)
• Excitation source same as Raman Imaging • Tiff (.tif)
• Patented Duet Vision Technology enables simultaneous • Microsoft Video (.avi)
Raman imaging and Raman dispersive measurements
Available FALCON II Configurations
ChemImage offers four different possible FALCON II configurations to make it easy for you to choose the equipment that’s
right for your applications and your budget. Below are side-by-side comparisons of four possible options, to help you
decide which FALCON II configuration is best suited for your needs. More detailed descriptions of each series can be found
on pages 7-10. If you have questions about any of the FALCON II series systems you can contact us at toll free at 1-877-
241-3550 or via email at info@chemimage.com.
FALCON II Professional Series FALCON II Plus Series
Description The Professional Series provides users with best wide- The Plus Series provides users with capabilities
field Raman chemical imaging capabilities available for both wide-field Raman chemical imaging and
including EMCCD cameras and a research grade spectroscopy capabilities.
(triple-grating) spectrometer.
The Plus Series systems are modular and can be
The Professional Series systems are modular and can upgraded later with higher sensitivity cameras, a
be upgraded later with additional imaging modalities. 3-grating spectrometer and additional imaging modalities.
Hardware • Optical microscope – cross polarized and • Optical microscope – cross polarized and
differential interference contrast imaging differential interference contrast imaging capable
capable • Scientific CCD for Raman imaging
• EMCCD for Raman imaging • RGB video imaging
• RGB video imaging • Fixed grating dispersive spectrometer with Duet
• Automated triple grating dispersive Vision Technology
spectrometer with Duet Vision Technology • Laser: 2-watt, 532 nm Nd:YAG laser
• Laser: 2-watt, 532 nm Nd:YAG laser • Option for Near-IR or Fluorescence
• Option for Near-IR or Fluorescence hyperspectral imaging
hyperspectral imaging
Software Tool • ChemImage Xpert™ software • ChemImage Xpert™ software
Time-to-Image • 1 minute • 10 minutes
Performance
Spectral •
Service & Support
After your installation is complete, our customer care department will assist you with any questions you may have.
Our dedicated staff will answer any service related questions as well as scheduling training. Customers receive 5
days of training on the FALCON II with their applications. We also take pride in our ability to quickly respond to each
customer in a timely manner. To learn more about our after purchase service and support you may call us toll free at
1-877-241-3550 or via email at info@chemimage.com.
FALCON II Standard Series FALCON II Lite Series
The Standard Series offers users the best wide-field The Lite Series is a low cost option to bring in the best
Raman chemical imaging capabilities on the market wide-field Raman chemical imaging capabilities on the
today with a high sensitivity EMCCD camera. market into your laboratory.
The Standard Series systems are modular and can The Lite Series systems are modular and can be
be upgraded later with spectrometers and additional upgraded later with higher sensitivity cameras,
imaging modalities. spectrometers and additional imaging modalities.
• Optical microscope – cross polarized and differential • Optical microscope – cross polarized and differential
interference contrast imaging capable interference contrast imaging capable
• EMCCD for Raman imaging • Scientific CCD for Raman imaging
• RGB video imaging • RGB video imaging
• Laser: 2-watt, 532 nm Nd:YAG laser • Laser: 2-watt, 532 nm Nd:YAG laser
• Option for Near-IR or Fluorescence hyperspectral imaging • Option for Near-IR or Fluorescence hyperspectral imaging
• ChemImage Xpert™ software • ChemImage Xpert™ software
• 1 minute • 10 minutes
• 2.1 cm-1 + 0.6 • 2.1 cm-1 + 0.6
FALCON II Professional Series
Overview
The Professional Series provides users with the best wide-field
Raman chemical imaging capabilities available including EMCCD
cameras and a research grade (triple-grating) spectrometer.
The Professional Series systems are modular and can be
upgraded later additional imaging modalities.
Hardware & Software
• Optical microscope for brightfield, cross polarized and
differential interference contrast imaging
• Hyperspectral imaging channel for Raman chemical
imaging with liquid crystal based tunable filter and EMCCD
• Raman spectroscopy channel: Triple-grating spectrometer
with Duet Vision Technology
• RGB video imaging
• Laser: 2-watt, 532 nm Nd:YAG laser
• Software: ChemImage XpertTM
• And more
Time-to-Image Performance 1 minute
Spectral Performance
FALCON II Plus Series
Overview
The Plus Series provides users with capabilities for both wide-
field Raman chemical imaging and spectroscopy capabilities.
The Plus Series systems are modular and can be upgraded
later with higher sensitivity cameras, a triple-grating
spectrometer and additional imaging modalities.
Hardware & Software
• Optical microscope for brightfield, cross polarized and
differential interference contrast imaging
• Hyperspectral imaging channel for Raman chemical imaging
with liquid crystal based tunable filter and scientific CCD
• Raman spectroscopy channel: Fixed, single-grating
spectrometer with Duet Vision Technology
(7-9 cm-1 FWHM resolution 250-3700 cm-1)
• RGB video imaging
• Laser: 2-watt, 532 nm Nd:YAG laser
• Software: ChemImage XpertTM
• And more
Time-to-Image Performance 10 minutes
Additional Imaging Modalities
Spectral Performance 1.0 cm-1 + 0.3
Users may also upgrade their imaging capabilities by
purchasing additional hyperspectral imaging modalities to
Upgrade Pathway be added to their existing system. Users may choose either
Electron Multiplication CCD Near-IR or Fluorescence hyperspectral imaging depending on
which best suits their application needs.
Users may upgrade their hyperspectral imaging capabilities by
purchasing an Electron Multiplication CCD (EMCCD) camera. Triple-Grating Spectrometer
This camera provides significant sensitivity enhancement over Users may upgrade their overall Raman spectroscopic
the standard CCD camera. Due to this increase in sensitivity, capabilities by adding a triple- grating spectrometer.
users can also increase speed of data acquisition. Upgrading to include this option allows users to study
materials in more detail without having to move the sample
from one instrument to another.
FALCON II Standard Series
Overview
The Standard Series offers users the best wide-field Raman
chemical imaging capabilities on the market today with a high
sensitivity EMCCD camera.
The Standard Series systems are modular and can be upgraded
later with spectrometers and additional imaging modalities.
Hardware & Software
• Optical microscope for brightfield, cross polarized and
differential interference contrast imaging
• Hyperspectral imaging channel for Raman chemical
imaging with liquid crystal based tunable filter and
EMCCD device
• RGB video imaging
• Laser: 2-watt, 532 nm Nd:YAG laser
• Software: ChemImage XpertTM
• And more
Time-to-Image Performance 1 minute
Spectral Performance 2.1 cm-1 + 0.6
Upgrade Pathway
Additional Imaging Modalities
Users may also upgrade their hyperspectral imaging
capabilities by purchasing additional imaging modalities to
be added to their existing system. Users may choose either
Near-IR or Fluorescence hyperspectral imaging depending on
which best suits their application needs.
Single or Triple-Grating Spectrometer
Users may upgrade their overall Raman spectroscopic
capabilities by adding either a single- or triple- grating
spectrometer. Upgrading to include this option allows users to
study materials in different ways without having to move the
sample from one instrument to another.FALCON II Lite Series
Overview
The Lite Series is a low cost option to bring in the best
wide-field Raman chemical imaging capabilities on the
market into your laboratory.
The Lite Series systems are modular and can be upgraded
later with higher sensitivity cameras, spectrometers and
additional imaging modalities.
Hardware & Software
• Optical microscope for brightfield, cross polarized and
differential interference contrast imaging
• Hyperspectral imaging channel for Raman chemical imaging
with liquid crystal based tunable filter and scientific CCD
• RGB video imaging
• Laser: 2-watt, 532 nm Nd:YAG laser
• Software: ChemImage Xpert™
• And more
Time-to-Image Performance 10 minutes
Spectral Performance 2.1 cm-1 + 0.6
Upgrade Pathway Single or Triple-Grating Spectrometer
Users may upgrade their overall Raman spectroscopic
Electron Multiplication CCD
capabilities by adding either a single- or triple- grating
Users may upgrade their hyperspectral imaging capabilities by spectrometer. Upgrading to include this option allows users to
purchasing an Electron Multiplication CCD (EMCCD) camera. study materials in different ways without having to move the
This camera provides significant sensitivity enhancement over sample from one instrument to another.
the standard CCD camera. Due to this increase in sensitivity,
users can also increase speed of data acquisition.
Additional Imaging Modalities
Users may also upgrade their hyperspectral imaging capabilities
by purchasing additional imaging modalities to be added to
their existing system. Users may opt for either Near-IR or
Fluorescence hyperspectral imaging depending on which best
suits their application needs.ChemImage Corporation
7301 Penn Avenue, Pittsburgh, PA 15208
Toll Free: 1.877.241.3550, Tel: 412.241.7335, Fax: 412.241.7311
info@chemimage.com, www.chemimage.com
PSREV004 10/2010
© 2010 ChemImage Corp. All Rights Reserved. ChemImage products and services are protected by U.S.and International issued and pending patents.You can also read
