DICLOFENAC EPOLAMINE TOPICAL PATCH FOR THE TREATMENT OF PAIN - Biolife
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JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 435-441 (2018)
EDITORIAL
DICLOFENAC EPOLAMINE TOPICAL PATCH FOR THE TREATMENT OF PAIN
G. AFFAITATI1, M.A. GIAMBERARDINO1, D. LAPENNA1 and R. COSTANTINI2
1
Department of Medicine and Science of Aging, “G. D’Annunzio” University of Chieti and Center
of Sciences of Aging and Translational Medicine, CeSI-MeT, Chieti, Italy; 2Institute of Surgical
Pathology, “G. D’Annunzio” University of Chieti, Chieti, Italy
Received January 19, 2018 – Accepted April 11, 2018
Topical nonsteroidal anti-inflammatory drugs produce local pain relief while avoiding systemic
adverse events, thanks to minimal systemic absorption. This review evaluates the effectiveness and
safety of a topical diclofenac preparation, diclofenac epolamine (DHEP) patch 1.3% or diclofenac
epolamine patch with heparin as excipient (DHEP+H) in treating mild-to-moderate pain. DHEP patch
was associated with significant pain relief and improved function in numerous pain conditions, from
minor soft tissue injuries to osteoarthritis and myofascial pain syndromes. Tolerability was good-to-
excellent in all studies, with no serious adverse events. DHEP+H further improved efficacy without
affecting tolerability. This patch is effective and safe for localized mild-to-moderate somatic pain.
0393-974X (2018)
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DISCLOSURE: ALL AUTHORS REPORT NO CONFLICTS OF
INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 443-447 (2018)
EDITORIAL
TIME TO LOOK PAST TNF AND THALIDOMIDE FOR CACHEXIA - COULD MAST
CELLS AND FLAVONOIDS BE THE ANSWER?
L. KING1, I. TSILIONI2 and T.C. THEOHARIDES2,3,4
1
Friedman School of Nutrition, Tufts University, Boston, MA, USA; 2Molecular Immunopharmacology
and Drug Discovery Laboratory, Department of Immunology, Tufts University School of Medicine,
Boston, MA, USA; 3Sackler School of Graduate Biomedical Sciences, Tufts University, Boston, MA,
USA; 4Department of Internal Medicine, Tufts University School of Medicine and Tufts Medical
Center, Boston, MA, USA
Received May 17, 2018 - Accepted June 10, 2018
Cachexia is a wasting condition associated with late stages of many chronic illnesses and may be
present in up to 80% of patients with advanced cancers. Cachexia is a metabolic derangement resulting
in a disturbance to the homeostasis of muscle breakdown and synthesis, favoring catabolism and muscle
loss. Despite making strides in treating cancer itself, there have been no major advances in the treatment
of cachexia pharmacologically or nutritionally. Clinical trials using anti-TNF biologics and thalidomide
have largely failed. A new approach may be to focus on other possible waste-inducing mediators, possibly
derived from mast cells, and the beneficial action of select natural flavonoids.
0393-974X (2018)
Copyright © by BIOLIFE, s.a.s.
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reproduced without written permission from the copyright holder.
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DISCLOSURE: ALL AUTHORS REPORT NO CONFLICTS OF
INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 449-454 (2018)
EDITORIAL
NEW CONCEPTS IN NEUROINFLAMMATION: MAST CELLS
PRO-INFLAMMATORY AND ANTI-INFLAMMATORY CYTOKINE MEDIATORS
Al. CARAFFA1, C. CONTI2, C. D’OVIDIO3, C.E. GALLENGA4, L. TETTAMANTI5,
F. MASTRANGELO6, G. RONCONI7, S.K. KRITAS8 and P. CONTI9
Department of Pharmacology, University of Perugia, Perugia, Italy; 2Department of
1
Psychological, Health, and Territorial Sciences, University “G. d’Annunzio” of Chieti-Pescara,
Chieti, Italy; 3Section of Legal Medicine, Department of Medicine and Aging Sciences, “G.
d’Annunzio” University of Chieti–Pescara, Italy; 4Department of Biomedical Sciences and
Specialist Surgery, Section of Ophthalmology, University of Ferrara, Italy; 5Department of Medical
and Morphological Science, University of Insubria, Varese, Italy; 6Department of Medical Science
and Biotechnology, University of Foggia, Foggia, Italy; 7UOS Clinica dei Pazienti del Territorio,
Policlinico Gemelli, Rome, Italy; 8Department of Microbiology and Infectious Diseases, Aristotle
University of Thessaloniki, Macedonia, Greece; 9Immunology Division, Postgraduate Medical
School, University of Chieti-Pescara, Chieti, Italy
Received May 19, 2018 - Accepted June 6, 2018
The activation of brain nociceptors and neurons may lead to neurogenic inflammation, an event
that involves immune cells including mast cells (MCs). Microglia are similar to macrophages and
secrete pro-inflammatory IL-1 family members and TNF. TNF is rapidly released (first 10 minutes
from MC granules) and is subsequently secreted along with other pro-inflammatory cytokines with
a new synthesis after several hours. MC-derived TNF is a very powerful pro-inflammatory cytokine
which mediates sensitization of the meningeal nociceptors. Here, we report the involvement of MCs in
neuroinflammation, the role of inflammatory cytokine IL-1 family members, and of TNF, as well as the
potential inhibition of IL-37.
0393-974X (2018)
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reproduced without written permission from the copyright holder.
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DISCLOSURE: ALL AUTHORS REPORT NO CONFLICTS OF
INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 455-464 (2018)
EFFECTS OF SOYBEAN ISOFLAVONES ON Wnt/β-CATENIN AND THE TGF-β1
SIGNALING PATHWAY IN RENAL TISSUE OF TYPE 2 DIABETIC RATS
CL. LIU1*, L. YAN1*, KR. CAI1, K. SUN2, Y. QI3, YL. HAN2,
XD. ZHANG4 and XD. SUN1
Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang,
1
Heilongjiang, People’s Republic of China; 2Department of Biology, Mudanjiang Medical University,
Mudanjiang, Heilongjiang, People’s Republic of China; 3Department of Public Health, Mudanjiang
Medical University, Mudanjiang, Heilongjiang, People’s Republic of China; 4Department of
Physiology, Mudanjiang Medical University, Mudanjiang, Heilongjiang, People’s Republic of China
*
These authors contributed equally to this work
Received December 8, 2017 – Accepted March 12, 2018
To observe the effect of Soyisoflavones (SI) on the expression of Wnt/β-catenin signaling pathway elements,
transforming growth factor-β (THGF-β) and its related proteins in the renal interstitia of diabetic nephropathic
(DN) rats, 48 DN rats were randomly divided into 4 groups: DN model group (group DN), soybean isoflavone
treatment group (group DA), DN model group + losartan treatment group (group DL), DN model group + soybean
isoflavones combined with losartan treatment group (group SL). Each group comprised 12 rats. Twelve healthy
Wistar rats were selected as normal controls (group N). After 12 weeks of continuous administration of soybean
isoflavone or losartan or those two combined, the body weight of rats was recorded and serum urea nitrogen (BUN)
and creatinine (Scr) were measured. The expression of Wnt4, β-catenin, and TGF-β1 proteins, as well as mRNA,
in the renal interstitium were detected by immunohistochemistry and real-time quantitative PCR (FQ-PCR). In
all the groups, Wnt4, β-catenin and TGF-β1 protein were only expressed in renal interstitial and renal tubular
epithelial cells. There was no significant difference between group DA and group DL (P> 0.05). FQ-PCR results
showed that Wnt4, β-catenin and TGF-β1 mRNA were consistent with the expression of these proteins in the renal
tissue of each group. Soy isoflavones can reduce 24-h urinary protein quantification, alleviate renal interstitial
pathological damage, and regulate the expression of Wnt4, β-catenin and TGF-β1 in the renal interstitium. This
suggests that soybean isoflavones could delay the process of renal interstitial fibrosis in DN rats by decreasing the
expression of Wnt4, β-catenin and TGF-β1 in the renal interstitium, thus demonstrating that soybean isoflavones
plus losartan have the best protective effects against diabetes-induced renal fibrosis.
1
0393-974X (2018)
Copyright © by BIOLIFE, s.a.s.
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reproduced without written permission from the copyright holder.
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DISCLOSURE: ALL AUTHORS REPORT NO CONFLICTS OF
INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 465-478 (2018)
THE ROLE FOR CYCLIC GLYCINE-PROLINE, A BIOLOGICAL REGULATOR OF
INSULIN-LIKE GROWTH FACTOR-1 IN PREGNANCY-RELATED OBESITY AND
WEIGHT CHANGES
J. GUAN1,2,3, G. SINGH-MALLAH2,3,4, K. LIU1,2, E. THORSTENSEN4, P. SHORTEN5,6,
E.A. MITCHELL7, R. TAYLOR8, P. HARRIS9, M. BRIMBLE9,3, J.M.D. THOMPSON7
and R. MURPHY10
Department Pharmacology and Clinical Pharmacology, School of Medical Sciences, Faculty of
1
Medicine and Health Sciences, University of Auckland, Auckland, New Zealand;
2
Centre for Brain Research, Faculty of Medicine and Health Sciences, University of Auckland,
Auckland, New Zealand; 3Brain Research New Zealand, New Zealand; 4The Liggins Institute, University
of Auckland, Auckland, New Zealand; 5AgResearch Ltd, Ruakura Research Centre, Hamilton, New
Zealand; 6Riddet Institute, University of Massey, Palmerston North, New Zealand; 7Department of
Paediatrics, Child and Youth Health, School of Medicine, Faculty of Medicine and Health Sciences,
University of Auckland, Auckland, New Zealand; 8Department of O&G, School of Medicine, Faculty
of Medicine and Health Sciences, University of Auckland, Auckland, New Zealand; 9Department
of Medicinal Chemistry, School of Chemistry, University of Auckland, Auckland, New Zealand;
10
Department of Medicine, School of Medicine, University of Auckland, Auckland, New Zealand
Received October 16, 2017 – Accepted March 26, 2018
Cyclic Glycine-Proline (cGP) regulates the homeostasis of insulin-like growth factor (IGF)-1 function and
cGP/IGF-1 ratio determines IGF-1 bioactivity in vitro and in vivo. Plasma IGF-1 represents largely inactive
IGF-1 and weakly associated with human obesity and hypertension. We evaluated the regulatory role for cGP
in pregnancy-related obesity and hypertension, and in obesity status between pregnancy and postpartum.
Women were recruited in their first pregnancy. A cross-sectional study compared plasma concentration
of cGP, IGF-1 and IGF binding protein (IGFBP)-3 in women with obesity and/or hypertension to normal
controls 6-year postpartum using UPLC-MS and ELISA. A longitudinal study compared the changes of
these peptides from 15-week gestation to 6-year post-partum in the women who remained normal weight,
remained obese or changed to obese or to normal respectively. Study 1 is a cross-sectional study. The obese
group had lower IGF-1(p = 0.001), higher cGP/IGF-1 ratio (p = 0.0055) and the hypertensive group had
lower IGFBP-3 (p = 0.046) and cGP (p = 0.043) than the controls. Study 2 is a longitudinal study. Women
with weight loss had increased cGP/IGF-1 ratio (p = 0.0026) and decreased IGFBP-3 (p = 0.0001) compared
with women whose weight remained normal. Women with weight gain had lower IGFBP-3 (p < 0.0001) only.
Women who remained obese had increased cGP/IGF-1 ratio (p = 0.006) only. Increase in cGP/IGF-1 ratio is
associated with obesity, but not hypertension. Changes of IGFBP-3 and/or cGP/IGF-1 ratio are associated
with weight changes. The data suggest the role for cGP in obesity through autocrine regulation of IGF-1.
0393-974X (2018)
Copyright © by BIOLIFE, s.a.s.
This publication and/or article is for individual use only and may not be further
reproduced without written permission from the copyright holder.
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DISCLOSURE: ALL AUTHORS REPORT NO CONFLICTS OF
INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 479-487 (2018)
EXPRESSION OF PROTEOGLYCANS IN TWO TYPES OF AMELOBLASTOMA: NOVEL
IMMUNOHISTOCHEMICAL FINDINGS
Z. GÓMEZ-HERRERA1, N. MOLINA-FRECHERO2, P. DAMIÁN-MATSUMURA3,
R. GONZÁLEZ-GONZÁLEZ4, J.E. FARFÁN-MORALES5 and R. BOLOGNA-MOLINA6
1
Biological and Health Sciences Ph.D Program, Metropolitan Autonomous University (Universidad
Autónoma Metropolitana) Mexico City, Mexico; 2Health and Care Department, Metropolitan
Autonomous University (Universidad Autónoma Metropolitana- Iztapalapa) Mexico City, Mexico;
3
Department of Biology of Reproduction, Metropolitan Autonomous University (Universidad
Autónoma Metropolitana-Xochimilco) Mexico City, Mexico; 4Research Department, Juarez
University of the Durango State, Durango, Mexico; 5Molecular Pathology Laboratory, National
Institute of Pediatrics, Mexico City, Mexico; 6Molecular Pathology Area, School of Dentistry,
University of the Republic (UDELAR), Montevideo, Uruguay
Received January 6, 2018 – Accepted March 28, 2018
Alterations in cellular and extracellular matrix components play an important role during
tumorigenesis; proteoglycans are included among these components. Ameloblastomas are odontogenic
tumors distinguished as invasive and infiltrative neoplasms and are divided into different histological
types, the most common of which are the unicystic ameloblastoma and the conventional ameloblastoma.
The aim of this study was to identify the presence of two proteoglycans, perlecan and biglycan, in different
types of ameloblastoma. Using immunohistochemistry, we determined the presence of both proteins in 28
unicystic ameloblastomas and 23 conventional ameloblastomas. We identified the cytoplasmic and nuclear
presence of perlecan and the cytoplasmic presence of biglycan in both types of ameloblastoma. The mean
values of immunoexpression were higher in the conventional type compared to the unicystic type. Neither
the presence of biglycan in ameloblastomas nor the nuclear presence of perlecan in any odontogenic tumor
has previously been reported. The differential immunoexpression of perlecan and biglycan in these types
of ameloblastomas suggests their participation in the developmental process of these tumors.
0393-974X (2018)
Copyright © by BIOLIFE, s.a.s.
This publication and/or article is for individual use only and may not be further
reproduced without written permission from the copyright holder.
Unauthorized reproduction may result in financial and other penalties
DISCLOSURE: ALL AUTHORS REPORT NO CONFLICTS OF
INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 489-495 (2018)
TETRAMETHYLPYRAZINE INHIBITED HYPOXIA-INDUCED EXPRESSION OF
CALCIUM-SENSING RECEPTORS IN PULMONARY ARTERY SMOOTH MUSCLE
CELLS IN CHICKENS
H. ZHANG1,2,3*, Z. CHANG3*, K. MEHMOOD3,4, M.K. YANG1,2, Z. LIU1,2, Z. DUAN1,2,
F. YUAN1,2, M.M. ALI5, M. ADNAN6, M.U. QASIM6, S. SHAHEEN7, R.Z. ABBAS8,
Y. TIAN1,2 and R. GUO1,2
Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan, China;
1
2
Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture)
Wuhan, China; 3College of Veterinary Medicine, Huazhong Agricultural University, Wuhan,
China; 4University College of Veterinary and Animal Sciences, Islamia University of Bahawalpur,
Pakistan; 5University of Veterinary and Animal Sciences Lahore, Pakistan; 6College of Plant Science,
Huazhong Agricultural University, Wuhan, P. R. China; 7Bahauddin Zakariya University Multan,
Pakistan; 8Departmet of Parasitology, University of Agriculture Faisalabad, Pakistan
Received March 1, 2018 – Accepted March 21, 2018
*
These authors made equal contributions to this article.
Tetramethylpyrazine (TMP) is a biologically active ingredient, which is isolated from a popular
Chinese medicinal plant. It has been used effectively to treat ischemic heart problems, cerebrovascular
and thrombotic vascular diseases. This study was designed to evaluate the effect of TMP on calcium-
sensing receptors in pulmonary artery smooth muscle in chickens. For this purpose forty day-old
chicks were distributed into five groups: the control group, the hypoxia group (kept under low Oxygen
treatment), and TMP groups (kept under low Oxygen treatment along with treatment of different
concentrations of TMP). The pulmonary artery smooth muscle cells were also cultured on 6-well plates
in high glucose culture medium and divided into the same five groups. We used in vivo and in vitro study
models by applying immunohistochemistry, RT-qPCR assay and Western blotting analysis. Our results
showed that pre-incubation with hypoxia markedly stimulated the activation of calcium-sensing receptor
(CaSR) in pulmonary artery smooth muscle cells (PASMCs). The TMP decreased the mRNA and protein
levels of CaSR. Treatment with TMP clearly inhibited the activation of all CaSR in a dose-dependent
manner. Our data demonstrated that TMP can down-regulate the expression of CaSR. Therefore, these
findings provide a new target to treat pulmonary arterial hypertension (PAH) under hypoxic conditions.
0393-974X (2018)
Copyright © by BIOLIFE, s.a.s.
This publication and/or article is for individual use only and may not be further
reproduced without written permission from the copyright holder.
Unauthorized reproduction may result in financial and other penalties
DISCLOSURE: ALL AUTHORS REPORT NO CONFLICTS OF
INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 497-505 (2018)
REGULATION OF VITAMIN D RECEPTOR AND GENISTEIN ON BONE
METABOLISM IN MOUSE OSTEOBLASTS AND THE MOLECULAR MECHANISM OF
OSTEOPOROSIS
CF. YE1, YM. PAN1 and H. ZHOU2
Department of Orthopaedics, Tonglu County Chinese Medicine Hospital, Hangzhou, China;
1
2
Department of Orthopaedics, Hangzhou Traditional Chinese Medicine Hospital, Hangzhou, China
Received February 8, 2018 – Accepted April 10, 2018
The first two authors contributed equally to this work
The aim of this work was to study the mechanisms of vitamin D receptor (VDR) and Genistein (Gen)
on the regulation of bone metabolism of phytoestrogens from cellular and epidemiological perspectives.
MC3T3-E1 cells were treated with different concentrations of Gen, and the cell-proliferation rate was
detected by an MTT colorimetric assay. The effect of the VDR receptor blocker ZK159222 on the Gen
effect was then observed; after adding Gen to MC3T3-E1 cells, we detected the expression of VDR protein
via Western blotting. After adding estrogen receptor α-blocker MPP and estrogen receptor β-blocker
PHTPP, we observed the effect of Gen on the regulation of the VDR protein. DNA was extracted from
the blood samples of 200 postmenopausal women in the early epidemiological survey, and the restriction
fragment length polymorphism of VDR gene Apa I and Bsm I in each sample was observed. The results
were analyzed using dietary survey and bone mineral density examination. The results show that
10-8mol/L Gen can promote the proliferation of MC3T3-E1 cells (P
0.05). In addition, there was no significant correlation between dietary phytoestrogen intake and BMD
in different genotypes (P> 0.05). In conclusion, VDR can mediate the effect of Gen on the proliferation
of MC3T3-E1 cells. The up-regulated expression of VDR protein in Gen is not mediated by the estrogen
receptor. Moreover, the VDR gene polymorphism is not related to the BMD in various parts and is not
related to the bone metabolism effect of the dietary plant estrogen intake.
0393-974X (2018)
Copyright © by BIOLIFE, s.a.s.
This publication and/or article is for individual use only and may not be further
reproduced without written permission from the copyright holder.
Unauthorized reproduction may result in financial and other penalties
DISCLOSURE: ALL AUTHORS REPORT NO CONFLICTS OF
INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 507-516 (2018)
miR-145 IS CRITICAL FOR MODULATION OF VASCULAR SMOOTH MUSCLE CELL
PROLIFERATION IN HUMAN CAROTID ARTERY STENOSIS
Z. HAN1,2, H. HU3, M. YIN4,5, X. LI4, J. LI6, L. LIU1,2 and B. LIU1
Department of General Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin,
1
China; 2Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Department of General
Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin, China; 3Department
of Vascular and Thyroid Surgery, the First Hospital of China Medical University, Shenyang,
China; 4Department of Dermatology, Xiangya Hospital, Central South University, Changsha,
China; 5Pathology and the Vascular Biology and Therapeutics Program, Yale University School of
Medicine, New Haven, Connecticut, USA; 6Department of Endodontics, the First Affiliated Hospital
of Harbin Medical University, Harbin, China
Received March 3, 2018 – Accepted March 21, 2018
miR-145 is highly expressed in vascular cells, where it regulates phenotypic switching and vascular
homeostasis, but its role in carotid artery stenosis (CAS) is controversial. In the present study, the
expression of miR-145 was assessed by real time quantitative reverse transcriptase polymerase chain
reaction (qRT-PCR) in human samples (both plasma and/or endarterectomy samples) from patients with
symptomatic CAS and in controls without CAS. The mouse carotid artery ligation (CAL) model was used
to determine the role of miR-145 on vascular smooth muscle cells in vivo (VSMCs) by using a mimic of or
an inhibitor of miR-145. We found that miR-145 expression was significantly reduced in the plasma and
plaque from patients with CAS (pJOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 517-527 (2018)
AMINO ACID AND HYALURONIC ACID MIXTURES DIFFERENTIALLY REGULATE
EXTRACELLULAR MATRIX GENES IN CULTURED HUMAN FIBROBLASTS
B. DE SERVI1, A. ORLANDINI2, E. CAVIOLA1 and M. MELONI1
1
VitroScreen – In Vitro Research Laboratories, Milan, Italy; 2Professional Dietetics, Milan, Italy
Received October 18, 2017 – Accepted March 15, 2018
The aim of this screening study was to evaluate the efficacy of different proprietary mixtures of amino
acid and hyaluronic acid (HA) in stimulating the production of extracellular matrix (ECM) components,
particularly the neo-synthesis of elastin, and in promoting a more efficient deposition of elastic fibres
(elastogenesis), while at the same time maintaining the stimulation of collagen. The study has allowed
identification of the optimal ratios between the amino acids (AA) for the production of collagen and elastin.
Human primary dermal fibroblasts from a 44-year-old female donor were used as a test system in an
experimental design based on the evaluation of the expression of relevant ECM genes using a transcriptomic
dynamic approach. The expression of ECM genes was evaluated by RTqPCR from 24 to 120 hours in the
presence of the test items. Moreover, the production of ECM proteins was verified by Western blot analysis
after a 120 h treatment period. In addition to elastin, collagen IV, a fundamental structural component of the
basal lamina responsible for epithelial and connective tissue anchoring, was analysed as potential target for
the modulation of ECM protein production by human fibroblast. The first phase of the study demonstrated
that alanine and valine are essential to promote production of elastin, of which they are important constituents.
The second phase of the study, which was conducted to clarify the interactions between the different clusters
of AA, demonstrated that it is necessary to choose a mixture that contains specific amounts of amino acids of
both proteins, collagen and elastin, to give a significant response and a significant production of both. This also
proves the existence of a ratio between the 2 clusters (AA elastin/AA collagen) that guarantees an adequate
and balanced response to gene expression and production by fibroblasts, collagen and elastin. The study has
allowed identification of the optimal ratios between the AA for the production of collagen and elastin.
0393-974X (2018)
Copyright © by BIOLIFE, s.a.s.
This publication and/or article is for individual use only and may not be further
reproduced without written permission from the copyright holder.
Unauthorized reproduction may result in financial and other penalties
DISCLOSURE: ALL AUTHORS REPORT NO CONFLICTS OF
INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 529-536 (2018)
ROLE OF APC-MEDIATED MDR-1/CLCX-1 SIGNALING PATHWAY IN OVARIAN TUMORS
Y. WANG, C. CAO, D. FANG and Y. HU
Department of Gynecology, Maternal and Child Health Care Hospital, Laiwu City, Shandong
Province, China
Received September 3, 2017 – Accepted April 17, 2018
We explored the role of APC-mediated MDR-1/CLCX-1 signaling pathway in ovarian tumors. In this
study, ovarian tumor cell lines SKOV-3, ES-2 and MCV-152 were used to conduct the research. Fluorescence
quantitative PCR and Western-blotting were used to investigate the effects of MDR-1/CLCX-1 signaling
pathway in ovarian tumors. The effects of the APC gene silencing and overexpression on proliferation and
apoptosis of ovarian tumor cells were detected by flow cytometry. Compared to normal cells, the expression
of APC gene mRNA in ovarian tumor cells was significantly decreased (pJOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 537-543 (2018)
DIFFERENCES IN EXPRESSION OF YKL-40 AND TLR4 IN NASAL SINUS MUCOSA OF
CHRONIC SINUSITIS PATIENTS WITH AND WITHOUT NASAL POLYPS
X.Z. LI1, S.C. ZHAO2, X.L. CAI1, Y.F. WANG2, J. CHEN2, X.F. MA2 and H. ZHANG2
Department of Otolaryngology, Qilu Hospital, Shandong University; Key Laboratory of
1
Otolaryngology, Chinese Ministry of Health, Shandong, China; 2Department of Otolaryngology,
Affiliated Hospital of Binzhou Medical University, Shandong, China
Received April 3, 2018 – Accepted April 17, 2018
This work studies the expression differences of YKL-40 and TLR4 in nasal sinus mucosa of chronic
sinusitis patients with and without nasal polyps and its clinical significances. Fifty chronic sinusitis
patients with nasal polyps and 50 chronic sinusitis patients without, accepted by our hospital during
February 2016-February 2017, were included and taken as group A and group B, respectively. In
addition, another 50 patients with nasal deviation were taken as group C (control group). The ostiomeatal
complex mucosa of group A and B and the inferior turbinate mucosa of group C were taken and the
fluorescence quantitative PCR method was applied to detect the expression of YKL-40, TLR4 and NF-
κB of the mucosa and explore and influence of YKL-40 and TLR4 on NF-κB. There was a negative
correlation between YKL-40 and TLR4 in group A, and the difference was statistically significant (PJOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 545-551 (2018)
THE INVOLVEMENT AND MECHANISM OF FEBUXOSTAT IN NON-ALCOHOLIC
FATTY LIVER DISEASE CELLS
W. TANG1, J. MU2, QI. CHEN3, X. LI4 and H. LIU1
Department of endocrinology, Xiangya Hospital, Central South University, Changsha, Hunan, China;
1
2
Department of Endocrinology, The First Hospital of Harbin, Harbin City, China; 3Department of
Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin City, China; 4Department
of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing City, China
Received March 9, 2018 – Accepted April 4, 2018
It has been proved that hyperuricemia is associated with non-alcoholic fatty liver disease (NAFLD).
The xanthine oxidase (XO) inhibitor, febuxostat, decreases free fatty acids-induced fat accumulation in
HFDT-fed mice. Here, it is shown that febuxostat attenuates fat accumulation and reactive oxygen species
(ROS) in HepG2 cells. It was further found that the underlying mechanism is related to the reduction
in expression of NLRP3/caspase-1/IL-18/IL-1beta and improved insulin resistance (IR). This finding
highlights the possible molecular pathways involving NLRP3 activation for management of ROS and
insulin IR. In conclusion, febuxostat may be a promising potential treatment for patients with NAFLD.
0393-974X (2018)
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DISCLOSURE: ALL AUTHORS REPORT NO CONFLICTS OF
INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 553-563 (2018)
DIFFERENT MOLECULAR SUBTYPES OF BREAST INVASIVE DUCTAL CARCINOMA
L.J. ZHENG, D. YANG, L.J. SUN, S.S. LI, J.Y. WANG and S.C. YE
Department of Oncology, Affiliated Hospital of Jining Medical University, Jining City, Shandong, China
Received February 10, 2018 – Accepted May 3, 2018
This study aims to analyze the clinical characteristics of breast invasive ductal carcinoma (BIDC)
in patients with different molecular subtypes and identify possible correlation to prognosis. miR-
10b expression level was detected using real-time quantitative polymerase chain reaction (RT-PCR).
Tissue sections were collected and stained using the immunohistochemical method. The samples were
grouped into human epidermal growth factor receptor 2, (HER2) overexpression, Triple negative,
Luminal A and Luminal B groups. Age, tumor size, breast cancer molecular subtype, clinical stage,
miR-10b positive expression, positive expression of Ki-67 and survival rate of patients diagnosed with
BIDC were analyzed. The expression of miR-10b was down-regulated in the breast carcinoma tissues.
Age and clinical stage were distinctly different among patients with different molecular subtypes of
BIDC (p < 0.05). Tumor size was not remarkably different (p > 0.05) among different subtypes.
The positive expression rate of miR-10b was lowest in patients with Luminal B BIDC; the positive
expression of Ki-67 was in different correlation with the expression of different receptors, and there
was a remarkable difference (p < 0.05); moreover, the survival rate of patients with Luminal A and
B BIDC was significantly higher compared to patients with other molecular subtypes (p < 0.05).
Clinical characteristics and prognosis of BIDC vary among different molecular subtypes. This study
provides valuable input on BIDC therapy.
0393-974X (2018)
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LETTER TO THE EDITOR
PERCUTANEOUS TRANSFORAMINAL ENDOSCOPIC DISCECTOMY AND MINI-
INCISION SURGERY IN THE TREATMENT OF LUMBAR INTERVERTEBRAL DISC
PROTRUSION
HX. NING1, YW. YUAN2, QY. ZHANG3, ZZ. SUN1, HY. NING4 and P. WANG1
1
Spine Surgery, Binzhou Medical University Hospital, Binzhou, China; 2Binzhou Hospital of
Traditional Chinese Medicine, Binzhou, China; 3Anesthesia Department, Binzhou Medical
University Hospital, Binzhou, China; 4Tianjin Haihe Hospital, Tianjin, China
Received August 28. 2017 – Accepted December 18, 2017
Lumbar intervertebral disc protrusion (LIDP) is a frequently occurring disease and 10-20% of
patients require surgical treatment. Percutaneous transforaminal endoscopic discectomy (PTED) and
mini-incision surgery are currently the most common surgeries for patients. To analyze the efficacy of
PTED and mini-incision surgery in the treatment of lumbar intervertebral disc protrusion, this study
selected 216 patients with LIDP who were admitted to the hospital between February 2014 and June 2015.
The subjects were randomly divided into an observation group and a control group, 108 each. Patients
in the observation groups were treated by PTED, while patients in the control group were treated by
mini-incision surgery, and treatment efficacy of the two groups was observed. The results demonstrated
that the duration of surgery and length of hospital stay of the observation group were significantly
shorter than those of the control group, the intraoperative blood loss of the observation group was
significantly less than that of the control group and the size of surgical incision of the observation group
was much smaller than that of the control group (PJOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 571-576 (2018)
LETTER TO THE EDITOR
PRE- AND POST-TREATMENT COMPUTED TOMOGRAPHIC FINDINGS OF A
PRIMARY INTRANASAL TRANSMISSIBLE VENEREAL TUMOR IN A CANINE PATIENT
M. PATSIKAS1, K. ADAMAMA-MORAITOU1, A. THOMAS2, C. SOULTANI2,
I. CHRISSOGONIDIS3, A. FOTIADOU4, G. TRIKOUPI1, P. PAPADOPOULOU1,
G. ILIA3, P. KOSMAS2, P. FARMAKIS2, T. ILIA3,
M. KRITSEPI1 and D. PARDALI1
1
School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece; 2Private Practice,
Thessaloniki, Greece; 3School of Medicine, Aristotle University of Thessaloniki, Greece; 4Royal
National Orthopaedic Hospital, Brockley Hill, Stanmore, Middlesex, UK
Received December 7, 2017 - Accepted March 2, 2018
A two-year-old, female intact, cross-breed dog presented with a two-month history of nasal discharge.
Computed tomography (CT) demonstrated obliteration of both nasal cavities by soft tissue density,
destruction of the nasal and ethmoidal turbinates, and lysis of the frontal and palatine bones and maxilla.
Frontal sinuses and maxillary recesses were obscured by soft tissue/fluid density. Histopathological
examination of the mass was diagnostic of transmissible venereal tumor. The dog was clinically normal
3 months after treatment initiation with vincristine sulphate and amoxicillin/clavulanate. Six months
after the completion of treatment no mass-like lesion was demonstrated in CT sections. Nasal cavities,
maxillary recesses and frontal sinuses were filled with air. The reticular turbinate nasal plexus appeared
atrophic with focal loss of the nasal turbinates on both sides. The ethmoidal turbinates were well-defined;
however, focal loss of turbinates was also seen. Lysis of the frontal and palatine bones were still evident.
0393-974X (2018)
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LETTER TO THE EDITOR
EFFECT OF SILDENAFIL ON PULMONARY HYPERTENSION ASSOCIATED WITH
LEFT HEART FAILURE
N. WU and G. YANG
Department of Geriatric Cardiology, The First Affiliated Hospital of Zhengzhou University,
Zhengzhou, China
Received October 1, 2017 – Accepted March 12, 2018
This study aims to evaluate the effect of sildenafil on pulmonary hypertension (PH) associated with
chronic left heart failure. Twenty patients with PH and left heart failure were divided into treatment
group (10 cases, with an oral dose of sildenafil 75 mg daily for 8 weeks) and control group (10 cases,
with treatment of cardiac glycosides, diuretics, angiotensin-converting enzyme inhibitor, angiotensin
II receptor blocker and beta-blockers). Left ventricular systolic function (LVEF), the pulmonary
artery systolic pressure (PH), the left ventricular fraction shortening (LVFS), the left atrium diameter
(LAD) and the left ventricular end-diastolic diameter (LVD) were measured by echocardiography, the
left ventricular mass index (LVMI) was also calculated. The level of N-terminal pro-brain natriuretic
peptide (NT-proBNP) was detected by electrochemiluminescence and high sensitivity C-reactive protein
(hsCRP) by immune transmission. The walking distance in 6-minute walk test (6-MWT) was calculated.
Before treatment, there were no significant differences in LVEF, LVFS, NT–proBNP, hsCRP, 6-MWT,
LVD, LAD and LVMI between the treatment group and control group. After four weeks intervention
in the treatment group, LVEF, FS and 6-MWT were significantly increased, while NT–proBNP, hsCRP,
6-MWT, LVD and LVMI were significantly decreased, when compared with the control group. In
conclusion, sildenafil can improve cardiac function and reduce pulmonary artery pressure. In addition,
it can attenuate myocardial remodeling through its anti-inflammatory effect.
0393-974X (2018)
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INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 583-588 (2018)
LETTER TO THE EDITOR
VEGF-A EXPRESSION IN SOFT TISSUES REPAIRED BY SHOCKWAVE THERAPY:
DIFFERENCES BETWEEN MODALITIES
T. SCHNURRER-LUKE-VRBANIC1, V. AVANCINI-DOBROVIC1, I. SOSA2,
O. CVIJANOVIC3 and D. BOBINAC3
1
University Hospital Centre Rijeka, Department of Physical and Rehabilitation Medicine, Rijeka,
Croatia; 2University of Rijeka, Medical Faculty, Department of Forensic Medicine and Criminalistics,
Rijeka, Croatia; 3University of Rijeka, Medical Faculty, Department of Anatomy, Rijeka, Croatia
Received December 7, 2017 – Accepted March 1, 2018
Shockwave therapy has found its place in the medical treatment of various diseases of the locomotor
system such as acute fracture, nonunion, chronic tendinitis and pseudarthrosis. Focused shock waves
enable maximum energy in the therapeutic zone, and depth of penetration can be adjusted. Radial
shockwave therapy primarily affects superficial tissues, so its application in medicine is doubtful. Our
study aimed to assess long bone fracture healing in regard to soft tissues. For this investigation, 84 female
Wistar rats were divided into a focused shockwave group (n=36), a radial shockwave group (n=36) and
a control group (n=12). Conclusively, long bone fracture repair was enhanced in the shockwave groups.
Comparison between focused shock waves and radial shock waves suggested that this latter strongly
stimulated the processes of the healing, as 75% of vascular spaces were VEGF-A positive on the 5th day
of bone healing, and 85% on the 22nd day of healing.
0393-974X (2018)
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INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 589-597 (2018)
LETTER TO THE EDITOR
PURE RED CELL APLASIA WITH T-CELL LARGE GRANULAR LYMPHOCYTIC
LEUKEMIA
G. SHI1, C-M. HU1, Q. YU1, N. YANG1, Z-S. XUE1, B. ZHAO1,
M. GUO1 and Y. ZHENG2
1
Department of Hematology and Oncology, The Second Hospital of Jilin University
2
Department of Anesthesia, China-Japan Union Hospital of Jilin University
Received February 3, 2018 – Accepted March 16, 2018
Pure red cell aplasia (PRCA) develops as a result of erythroid precursors failing to reach maturity in
the bone marrow, which eventually leads to anemia. Here we present a case of a 64-year-old Asian male
with a medical history of colorectal adenocarcinoma who had been treated with 6 cycles of oxaliplatin
and capecitabine four years ago. The patient was diagnosed with PRCA and T-cell large granular
lymphocyte leukemia.
0393-974X (2018)
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INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 593-597 (2018)
LETTER TO THE EDITOR
AUTOLOGOUS PLATELET-RICH PLASMA FOR TREATMENT OF VENOUS LEG
ULCERS: A PROSPECTIVE CONTROLLED STUDY
D. ETUGOV1, V. MATEEVA2 and G. MATEEV1
1
Department of Dermatology and Venereology, Medical University Sofia, Bulgaria; 2Department of
Dermatology and Venereology, Military Medical Academy Sofia, Bulgaria
Received December 5, 2017 – Accepted March 12, 2018
Venous leg ulcers (VLUs) are chronic difficult-to-treat wounds which affect around 1-2% of the world
population. Conventional methods for treatment such as mechanical debridement, occlusive dressings and
local antibiotics in case of infection, often lack effectiveness. Autologous platelet-rich plasma (PRP) is an
alternative method in the treatment of chronic wounds. PRP contains inflammatory mediators, growth
factors, and cytokines that modulate the wound microenvironment to create a better chance for healing.
The aim of this prospective clinical study was to evaluate the efficacy of intralesional injection of PRP in
the management of VLUs. This study included 23 patients with VLUs. For each patient, two ulcers located
in the same anatomical zone and at the same clinical stage were selected. One was treated with a single
application of autologous PRP. The other ulcer was used as a control and was treated by conventional
methods. The size of the ulcers was assessed at baseline (visit 0), 15 days (visit 1) and 30 days after the
procedure (visit 2). Results showed a significant reduction of the size of the ulcer both in the group treated
with PRP (mean surface 1368.2 mm2 at visit 0 and 596.3 mm2 at visit 2) and in the control group (mean
surface 880.3 mm2 at visit 0 and 582.8 mm2 at visit 2). Statistical analysis showed a significant change in the
size of the ulcer between visit 0 and visit 2 in both groups (р < 0.0001). The application of PRP in difficult-
to-treat venous leg ulcers may be a promising new method for therapy of this condition. The results of this
study correlate with the data from the majority of previous studies and confirm the effectiveness of PRP.
Nevertheless further research in the area is needed to evaluate the therapeutic significance of the method
and eventually show its superiority to conventional treatments in larger cohorts.
0393-974X (2018)
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INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 599-605 (2018)
LETTER TO THE EDITOR
COMPARATIVE STUDY OF METHOTREXATE AND HUMAN UMBILICAL CORD
MESENCHYMAL STEM CELL TRANSPLANTATION IN THE TREATMENT OF
RHEUMATOID ARTHRITIS
YH. WANG1, ZQ. YANG1, SF. ZHU1 and Y. GAO2
1
Department of Pharmacy, Affiliated Hospital of Hebei University, Baoding City, Hebei Province,
People’s Republic of China; 2Department of Nephropathy, Affiliated Hospital of Hebei University,
Baoding City, Hebei Province, People’s Republic of China
Received January 15, 2018 – Accepted March 14, 2018
In this study, a collagen-induced arthritis (CIA) model was established to simulate rheumatoid
arthritis (RA) using two intradermal injections of bovine type II collagen and Freund’s complete
adjuvant mixture given at two-week intervals. Subsequently, the transplantation of human umbilical
cord mesenchymal stem cells (hUC-MSCs) was used to treat RA and the treatment efficacy, as well as
the possible regulatory mechanism underlying hUC-MSC transplantation, was observed. During the
study, forty rats were randomly divided into four groups and their blood samples were collected at
different time points to measure levels of serum cartilage oligomeric matrix protein (COMP). Based
on the symptoms and pathological features of the rats, a total success rate of 83% was achieved by the
treatment. Furthermore, the improvement of joint symptoms was more obvious when methotrexate and
MSC transplantation were used. In summary, it was concluded that MSC transplantation relieved the
symptoms of arthritis by down-regulating the expression of COMP on the synovial membrane and in
the serum of CIA rats.
0393-974X (2018)
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INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 607-611 (2018)
LETTER TO THE EDITOR
EFFECTS OF HOUSEHOLD BLEACH ON SPUTUM SMEAR MICROSCOPY TO
CONCENTRATE ACID FAST BACILLI FOR THE DIAGNOSIS OF PULMONARY
TUBERCULOSIS
G. RASOOL1*, M. RIAZ1, Z. MAHMOOD2, R. MOHY-UD-DIN3, J. AKHTAR1 and I. JAVED4
Department of Allied Health Sciences, Sargodha Medical College, University of Sargodha, Sargodha-
1
Pakistan; 2Department of Biochemistry, Government College University, Faisalabad-Pakistan;
3
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore-
Pakistan; 4Department of Biochemistry, Government College Women University, Faisalabad-Pakistan
Received January 19, 2018 – Accepted March 22, 2018
Tuberculosis (TB) is one of the major public health problem among contagious diseases in Pakistan.
TB diagnosis mainly depends on sputum smear microscopy. The main objective of this study was to
evaluate the effects of household bleach on sputum smear microscopy to concentrate acid fast bacilli for
the diagnosis of pulmonary tuberculosis. Sputum specimens of 200 suspected TB patients were collected
for the study. Smears were prepared from the purulent part of sputum sample before and after bleach
treatment, heat fixed and stained with the ZN technique. The obtained data were analyzed by chi-squared
test using SPSS software. Out of 200 isolates, 22 (11%) patients had positive smears for acid fast bacilli
(AFB) by direct ZN staining. After treatment with household bleach (NaOCL) and centrifugation, the
number of AFB positive patients were increased from 22 (11%) to 37 (18.5%). The bleach-concentration
method for sputum samples significantly increased the TB detection rate as compared to direct sputum
smear microscopy. Thus, a shift from direct sputum microscopy to bleach-concentration technique
should be considered a better method for detection of AFB in sputum through smear microscopy.
0393-974X (2018)
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INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 613-618 (2018)
LETTER TO THE EDITOR
COMPARISON OF GEFITINIB AND PLATINUM-BASED CHEMOTHERAPY AND ONLY
PLATINUM-BASED CHEMOTHERAPY TO TREAT LUNG ADENOCARCINOMA
L. YANG1, JH. FAN2, LL. LIU2, Y. SU1, D. LU1, JY. HUANG1, H. ZHANG1, Y. LI1, HD. HUO1,
ZH. DU1 and GT. LIU3
1
Central Sterile Supply Department, Affiliated HongQi Hospital of Mudanjiang Medical University,
Mudanjiang City, Heilongjiang Province, China; 2Department of Pharmacy, Affiliated HongQi
Hospital of MuDanJiang Medical University, Mudanjiang City, Heilongjiang Province, China;
3
Department of Thoracic Surgery, Affiliated HongQi Hospital of Mudanjiang Medical University,
Mudanjiang City, Heilongjiang Province, China
Received January 24, 2018 – Accepted March 14, 2018
To study the curative effects and safety for patients who adopt both gefitinib and platinum-based
chemotherapy or only platinum-based chemotherapy in the treatment of lung adenocarcinoma, 80 EGFR
mutation-positive lung adenocarcinoma patients in stage IIIB/IV were divided into two groups. Half of
them received both gefitinib and standard chemotherapy (group A), and the others (group B) received
only standard chemotherapy. Overall response rate (ORR), disease control rate (DCR), progression-
free survival (PFS), overall survival (OS) and the related toxicities of both groups were recorded in
order to take certain nursing measures for a variety of toxicities. Next, statistical methods were used to
analyze the curative effects and safety of the two treatments. The results showed that ORR, DCR and
median progression-free (mPFS) survival of the two groups of patients showed no statistical difference
(P>0.05). However, group A (18.56 months) had a longer median overall survival (mOS) than group B
(14.87 months), which was of statistical significance (P0.05). In conclusion, the two treatments have similar safety, but lung adenocarcinoma
patients with drug resistance during stage IIIB/IV after using first-line gefitinib therapy have lower
survival benefits than patients who take both gefitinib and platinum-based chemotherapy.
0393-974X (2018)
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INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 619-625 (2018)
LETTER TO THE EDITOR
ANTI-TUMOR MECHANISM OF IL-21 USED ALONE AND IN COMBINATION WITH
5-FLUOROURACIL IN VITRO ON HUMAN GASTRIC CANCER CELLS
ZQ. FU, Q. ZHOU, S. ZHU and W. LIU
Department of General Surgery, Shi Dong Hospital, Shanghai City, People’s Republic of China
Received January 15, 2018 – Accepted March 14, 2018
To study the effect and related mechanism of IL-21 alone and in combination with 5-Fluorouracil on
the proliferation and growth, transferability, and apoptosis of gastric cancer cells, we cultivated gastric
cancer cell SGC-7901 and created four experimental groups with varying concentrations of IL-21 and
5-Fluorouracil: IL-21 group (IL-21 100ng/ml), semi-combination group (5-Fluorouracil 25μg/ml+IL-21
100ng/ml), 5-Fluorouracil group (5-Fluorouracil 50μg/ml), and combination group (5-Fluorouracil 50μg/
ml+IL-21 100ng/ml). The MTT (3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-di- phenytetrazoliumromide)
assay was used to detect the inhibitory effect of each group on the proliferation and growth of gastric
cancer cells. A scratch-wound assay was carried out to detect the inhibitory effect of each group
on transferability. TUNEL assay was used to detect the effect of each group on the apoptosis of the
gastric cancer cells, and Western blot was used to detect the expression of caspase-3, caspase-8, bcl-
2, and c-myc, which are the proteins related to apoptosis, after the drug effect in each group. The
results show that, compared to the 5-Fluorouracil group, the inhibitory effects after 24 h of the IL-21
group and the semi-combination group on SGC-7901 were weaker (P0.05). The scarification test showed that all groups could inhibit the
transferability of SGC-7901 and that the effect increased successively from the IL-21 group, the semi-
combination group, the 5-Fluorouracil group, to the combination group. The TUNEL assay indicated
that all groups could promote the apoptosis of SGC-7901. The percentage of cell apoptosis increased,
and the Western blot showed that the expression of caspase-3, caspase-8, and c-myc, respectively, in the
semi-combination group, the 5-Fluorouracil group, and the combination group increased successively
and that the successive increasing of c-myc was the most significant. The expression of bc1-2 tended
to decrease. In conclusion, IL-21 used alone and in combination with 5-Fluorouracil are anti-tumor
mechanisms in SGC-7901.
0393-974X (2018)
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INTEREST RELEVANT TO THIS ARTICLE.JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 627-633 (2018)
LETTER TO THE EDITOR
EFFECT OF MANNOSE ON THE LUNG FUNCTION OF RATS WITH ACUTE
PANCREATITIS
WF. ZHANG1*, ZT. LI1*, JJ. FANG1, GB. WANG1, Y. YU1, ZQ. LIU1, YN. WU1,
SS. ZHENG2 and L. CAI3
1
Surgical Intensive Care Unit, The 1st Affiliated Hospital of Zhejiang University, Hangzhou, China;
2
Surgery, The 1st Affiliated Hospital of Zhejiang University, Hangzhou, China; 3Department of
Critical Care Medicine, Hangzhou Hospital of Chinese Medicine Hangzhou, China
Received February 1, 2018 – Accepted March 27, 2018
These authors contributed equally to this work.
*
The present study aimed to investigate the mechanisms by which mannose protects the lung injury
induced in rats with acute pancreatitis (AP). An AP combined with Acute Lung Injury (ALI) model was
established. A total of 90 healthy adult male Sprague-Dawley rats (300±50g weight) were randomly divided
into three groups: sham operation group (SO group), severe acute pancreatitis lung injury group (SAP
group), and mannose intervention group (MT group). Subsequently, each group was divided into two
subgroups based on the time passed from intervention, namely 6 and 12 h. Each subgroup comprised 15
rats. The ratio of wet/dry weight of the lung tissue exhibited no significant change at different time points
in the SO group. This parameter was significantly increased in the SAP group compared with the SO group
at each time point of the treatment (PJOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 32, no. 3, 635-639 (2018)
LETTER TO THE EDITOR
SALINE CONDITIONS ALTER MORPHO-PHYSIOLOGICAL INTENSIFICATION IN
PURSLANE (PORTULACA OLERACEA L.)
S. ZAMAN1, S-B. SHAH2, Y-T. JIANG3 and S-Q. CHE1
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China; 2State Key
1
Laboratory of Microbial and Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong
University, Shanghai, China; 3Department of Geography, East China Normal University, Shanghai, China
Received November 16-2017 – Accepted March 1, 2018
In this study, primary investigations of selected cultivar of purslane named as Tall Green under
particular salinity stress were evaluated to understand the basic concept of different mechanisms of
physiological attributes which will play an important role for molecular and proteomic level research.
The evaluation of morphological and physiological attributes under 0 mM (without salt addition) 100
mM and 200 mM salt stress changed dramatically. The results showed high salt stress at 200 mM
significantly decreasing the morphological attributes and performance of leaves, stems, and roots. At
moderate salt stress levels, 100 mM, the ratio of Fv/Fm slightly increased compared to high stress.
In addition, salt stress significantly decreased the total chlorophyll content (chl a+b) at 200 mM. The
relative water content percentage was high at 0 mM. Moreover, the electrolyte leakage (EL) significantly
increased with increasing salinity stress compared to control 0 mM.
0393-974X (2018)
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