COHA Translational Fellowship Opportunity for Residency-Trained Veterinary Specialists Vector-borne Disease Epidemiology, Ecology, and Response: A ...
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COHA Translational Fellowship Opportunity
for Residency-Trained Veterinary Specialists
Vector-borne Disease Epidemiology, Ecology, and Response: A One
Health Approach to Tick-borne Disease in the Mid-Atlantic
Area of Research:
The Vector-Borne Disease Epidemiology, Ecology, and Response (VEER) Hub is a multi-
disciplinary research collaboration comprised of researchers and public health practitioners at
North Carolina State University (NC State) and the University of North Carolina at Chapel Hill
(UNC-CH). The primary goal of the collaboration is to develop and implement evidence-based,
surveillance, response, and control strategies to protect people, animals, and livelihoods from the
threat posed by vector-borne diseases.
University/Department:
The position will be based in the Vector-borne Disease Diagnostic Laboratory (VBDDL),
Department of Clinical Sciences and Comparative Medicine Institute (CMI) at NC State’s
College of Veterinary Medicine (CVM). The NC State-CVM is one of the top ranked veterinary
colleges in the US, with 150 faculty members and a 250-acre biomedical campus with state-of-
the-art facilities, including a Biosafety Level 3 laboratory. Fellows will have ample opportunities
to work with mentors and collaborators from the NC State-CVM, NC State Department of
Entomology and Plant Pathology, the UNC-CH School of Medicine, UNC-CH Gillings School
of Global Public Health, the NC Department of Health and Human Services (NCDHHS), and the
Department of Defense via a partnership with Fort Bragg.
Primary Mentor:
Barbara Qurollo, MS, DVM
Associate Research Professor
Department of Clinical Sciences-College of Veterinary Medicine
Vector-borne Disease Diagnostic Laboratory
North Carolina State University
baquroll@ncsu.edu
Mentor Team:
Ross M. Boyce, MD, MSc
Assistant Professor
Division of Infectious Diseases
University of North Carolina School of Medicine
roboyce@med.unc.edu
Michael H Reiskind, MPH, PhD
Associate Professor
Department of Entomology and Plant PathologyNorth Carolina State University mhreiski@ncsu.edu Natalie Bowman, MD, MPH Assistant Professor of Medicine Division of Infectious Diseases University of North Carolina School of Medicine nbowman@med.unc.edu Ed Breitschwerdt, DVM, DACVIM The Melanie S. Steele Distinguished Professorship in Medicine Professor, Internal Medicine Department of Clinical Sciences-College of Veterinary Medicine Vector-borne Disease Diagnostic Laboratory North Carolina State University ebbreits@ncsu.edu Description of Potential Research Project(s): The fellow will be part of a multi-institutional, inter-disciplinary team comprised of computational biologists, epidemiologists, entomologists, physicians, molecular biologists, and veterinarians assembled as part of the Vector-Borne Disease Epidemiology, Ecology, and Response (VEER) Hub in North Carolina. VEER will implement a strong translational research program for advancing our understanding of tick-borne disease (TBD) in NC and the Mid- Atlantic region. The fellow will be expected to lead an independent research project that applies a “One Health” approach to the challenge of TBD in NC and the Mid-Atlantic. Potential projects, pending the fellow’s interest, include: (1) establishing sentinel surveillance sites across NC counties of predicted high TBD transmission intensity and vector suitability, (2) conducting an epidemiological study of newly recognized ticks (e.g. Haemaphysalis longicornis) and emerging, zoonotic pathogens (e.g. Heartland virus) that may impact human and animal health, and (3) surveying TBD in humans, companion animals and reservoir hosts through participating households, clinics and wildlife processing stations, respectively, in NC. Hosts will be tested for a range of bacterial, protozoal and viral TBDs using serological and molecular identification (PCR, RT-PCR and/or NGS modalities). The fellow may also participate in testing intervention programs to minimize tick-host interactions with the goal of reducing the incidence of endemic (i.e. rickettsioses) and emerging (i.e. Lyme disease) pathogens. Additional Training Opportunities: As a fellow at NC State’s CVM, they will have access to seminars, symposia, writing workshops, and teaching opportunities. Specifically, fellows may attend and present at veterinary specialty rounds (at the Veterinary Teaching Hospital), the CVM Research Forum, the Postdoctoral Research Symposium, and the CMI Symposium; participate in grant writing
workshops and a monthly K Grant Writing Group through CVM’s Office of Research; teach veterinary and undergraduate students as a guest lecturer, facilitator, or through week-long electives for veterinary students (allows the fellow to design electives and practice teaching methods such as active and problem-based learning or flipping the classroom); mentor students through the VBDDL, mentors’ laboratories or through the CMI Summer Interdisciplinary Research Initiative; and participate in the Teaching and Communications Certificate program, Preparing the Professoriate program and Academy of Educators program. For further career development opportunities, NC State and UNC both offer Professional Development Series’ including the NC State Preparing Future Leaders program and the UNC Center for Faculty Excellence. Working with collaborators, the fellow will participate in seminars and weekly meetings through UNC-CH School of Medicine, Institute of Global Health and Infectious Diseases, and weekly Infectious Diseases Epidemiology and Ecology Laboratory meetings. The fellow will be allowed to shadow physicians in the Infectious Diseases Clinic and observe TBD management in human medicine. VEER will support travel to a professional conference each year, first authorship of manuscripts and grant writing opportunities for federal and state funding of projects. Fellowships are for 2 years and provide stipend and employee benefits at the NIH post-doctoral pay scale. Fellows may supplement their stipend with up to 25% effort towards clinical work, if such work is in alignment with the research and career development plan. All fellowships will have a start date of fall 2021. Biosketches of primary mentor and mentor team (NIH format, 4 pages):
OMB No. 0925-0001 and 0925-0002 (Rev. 03/2020 Approved Through 02/28/2023)
BIOGRAPHICAL SKETCH
Provide the following information for the Senior/key personnel and other significant contributors.
Follow this format for each person. DO NOT EXCEED FIVE PAGES.
NAME: Barbara Qurollo
eRA COMMONS USERNAME (credential, e.g., agency login): baquroll
POSITION TITLE: Associate Research Professor
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing,
include postdoctoral training and residency training if applicable. Add/delete rows as necessary.)
DEGREE Completion
(if Date FIELD OF STUDY
INSTITUTION AND LOCATION
applicable) MM/YYYY
The University of West Florida, Pensacola FL BS 05/1994 Biology
NC State University, Raleigh NC MS 05/1999 Microbiology
Colorado State University, Fort Collins, CO DVM 05/2010 Veterinary Medicine
Animal Emergency Center and Specialty Services, n/a 08/2011 Veterinary Internship
Glendale, WI
NC State University, Raleigh NC n/a 08/2015 Postdoctoral Fellowship
A. Personal Statement
My research efforts are directed at advancing our understanding of the epidemiological and clinicopathological
effects of vector-borne diseases, with interest in Ehrlichia, Leishmania and Babesia organisms. My most recent
projects are related to vector-borne pathogen surveillance and discovery of zoonotic and emerging infectious
diseases, as well as developing improved molecular diagnostic techniques for vector-borne pathogens. During
my postdoctoral fellowship, I focused on vector-borne disease prevalence studies, clinical manifestations of
tick-borne infections in companion animals, and developing molecular diagnostics for the enhanced detection
of intracellular pathogens, including new assays for Anaplasma, Ehrlichia, Babesia, Bartonella, Leishmania,
Mycoplasma, Apicomplexa, Trypanosoma cruzi, and Rickettsia species. As a Research Associate Professor,
co-direct the Vector-borne Disease Diagnostic Laboratory at NC State College of Veterinary Medicine and am
currently working on several projects focused on vector-borne pathogen surveillance and discovery in Peru
and Madagascar. Furthermore, I am collaborating with two groups to utilize and expand upon next-generation
sequencing to detect novel pathogens in both companion animals and wildlife. Recent and current
collaborators include the University of North Carolina-Microbiome Core Facility, Duke University, The Duke
Lemur Center, and Universidad Peruana Cayetano Heredia, The College of Veterinary Medicine, Western
University in CA, the Universidad del Zulia, Maracaibo in Venezuela; Dipartimento di Sanità Pubblica
Veterinaria Faculty of Veterinary Medicine, University of Bari in Italy; St. George’s University School of
Veterinary Medicine in Grenada; and Oregon State University College of Veterinary Medicine in OR.
B. Positions and Honors
2020-present Associate Research Professor, North Carolina State University, College of Veterinary Medicine,
Department of Clinical Sciences, Raleigh, NC
2015-2020 Assistant Research Professor, North Carolina State University, College of Veterinary Medicine,
Department of Clinical Sciences, Raleigh, NC
2012-2015 Postdoctoral Research Scholar, North Carolina State University, College of Veterinary Medicine,
Intracellular Pathogens Research Laboratory, Raleigh, NC2010-2011 Veterinary Internship in Emergency Medicine and Critical Care, Animal Emergency Center and
Specialty Services, Milwaukee, WI
2007-2010 Veterinary Student Research Assistant: Colorado State University (CSU), Animal Cancer
Center, Fort Collins, CO
2005-2006 Scientist: AstraZeneca, Cancer Bioscience Discovery, Waltham, MA
2002-2005 Senior Associate Scientist: AstraZeneca, Cancer Bioscience Discovery, Waltham, MA
2001-2002 Senior Research Associate: Cereon Genomics, Microbial Discovery, Cambridge, MA
1999-2001 Research Associate: Cereon Genomics, Microbial Discovery, Cambridge, MA
Recognitions
2014 Postdoctoral Scholar of the Year Award, Department of Clinical Sciences, NCSU
2014 Travel Grant Award, Center for Comparative Medicine and Translational Research, NCSU
2013 Travel Grant Award, Center for Comparative Medicine and Translational Research, NCSU
2010 Clinical Oncology Award, CSU
2009 Professional Veterinary Medicine student grant
C. Contributions to Science
My most significant scientific contribution has been my involvement in vector-borne pathogen surveillance and
discovery of zoonotic and emerging infectious diseases, as well as developing improved molecular diagnostic
techniques for vector-borne pathogen discovery. Notable contributions include identification of a prevalent
Babesia species (B. vulpes; aka B. microti-like) in dogs in North America that caused clinical disease that was
previously thought to only be in dogs in Europe. Genetic organization of B. vulpes is in a clade that is
genetically different from typical NA canine Babesia spp., where routine molecular diagnostic assays did not
detect this species. I have identified and highlighted a high prevalence of tick-borne disease in Madagascar’s
endangered lemur populations.
D. Additional Information: Research Support and/or Scholastic Performance
Recent List of Published Work (2018-2020)
Qurollo BA, Stillman BA, Beall MJ, Foster P, Hegarty BC, Breitschwerdt EB; Ramaswamy Chandrashekar.
Comparison of Anaplasma and Ehrlichia species-specific peptide ELISA assays to immunofluorescent assays
for serological diagnosis of canine anaplasmosis and ehrlichiosis. 2020. In press AJVR.
First and corresponding author
Ernst E, Qurollo B, Olech C, and Breitschwerdt E. Bartonella rochalimae, a Newly Recognized Pathogen in
Dogs. 2020. In Press JVIM
Co-author, minor contribution
von Fricken ME, Qurollo BA, Boldbaatar B, Wang YW, Jiang RR, Lkhagvatseren S, Koehler JW, Moore TC,
Nymadawa P, Anderson BD, Matulis G. Genetic diversity of Anaplasma and Ehrlichia bacteria found in
Dermacentor and Ixodes ticks in Mongolia. Ticks Tick Borne Dis. 2020;11(1):101316.
Co-author, major contribution
Tyrell JD, Qurollo BA, Tornquist SJ, Schlaich KG, Kelsey J, Chandrashekar R, Breitschwerdt EB. Molecular
identification of vector-borne organisms in Ehrlichia seropositive Nicaraguan horses and first report of
Rickettsia felis infection in the horse. Acta tropica. 2019; 200:105170.
Corresponding author
Barash NR, Thomas B, Birkenheuer AJ, Breitschwerdt EB, Lemler E and Qurollo BA. Prevalence of Babesia
spp. and clinical characteristics of Babesia vulpes infections in North American dogs. JVIM. 2019; (5):2075-81.
Corresponding author
Qurollo BA, Ramaswamy C, Buch J, Beall MJ, Yancey C, Caudil A, Comyn A, and EB Breitschwerdt. Clinical
and laboratory findings in 41 dogs naturally infected with Ehrlichia ewingii. JVIM. 2019; 33:618-629.First and corresponding author Birkenheuer AJ, Marr HS, Wilson JM, Breitschwerdt EB, Qurollo BA. Babesia gibsoni cytochrome b mutations in canine blood samples submitted to a US veterinary diagnostic laboratory. JVIM. 2018; 32:1965-1969. Co-author, minor contribution Vieira T, Qurollo BA, Mongruel A, Baggio RA, Vidotto O, Breitschwerdt EB, Viera RFC. Potentially same novel Ehrlichia species in horses in Nicaragua and Brazil. Emerg Infect Dis. 2018; 24:953. Co-author, major contribution Lado P, Qurollo B, Williams C, Junge R, Klompen H. The microbiome of Haemaphysalis lemuris (Acari: Ixodidae), a possible vector of pathogens of endangered lemur species in Madagascar. Ticks Tick Borne Dis. 2018; 9:1252-60. Co-author, minor contribution Qurollo BA, Larsen PA, Rakotondrainibe HH, Mahefarisoa K, Rajaonarivelo T, Razafindramanana J, Breitschwerdt EB, Junge RE, Williams CV. Molecular surveillance of novel tick-borne organisms in Madagascar's lemurs. Ticks Tick Borne Dis. 2018; 9:672-677. First and corresponding author Yancey CB, Diniz PP, Breitschwerdt EB, Hegarty BC, Wiesen C and Qurollo BA. Doxycycline treatment efficacy in dogs with naturally occurring Anaplasma phagocytophilum infection. J Small Anim Pract. 2018; 59: 286 – 293. Corresponding author My bibliography from NCBI is available here: https://www.ncbi.nlm.nih.gov/myncbi/1zO4fWZkqxUAz/bibliography/public/ Current Research Support IDEXX Laboratories, Inc. Salary support: Identify vector-borne infections and co-infections in companion animals and study associated clinical manifestations (2018-2021) Role: Recipient Completed Research Support Triangle Center for Evolutionary Medicine Pan W (PI), Qurollo BA (Co-I), Lana J (Co-I), Kays RW. (Co-I). Cutaneous Leishmaniasis in Peru: A One Health Working Group (2019-2020) Role: Co-I Comparative Medicine Institute, NC State B. Qurollo (PI), William Pan (PI), Micheal Levy (Co-I), Roland Kays (Co-I), Charles Nunn (Co-I), Maggie Skiles (Co-I), Justin Lana (Co-I). Untangling human-animal transmission of cutaneous leishmanaisis. Funded by NC State-Research Innovation Seed Funding, NCSU-Comparative Medicine Institute, and matching funds Mike Levy (2017-2018). Role: Co-PI American Kennel Club Canine Health Foundation P.P. Diniz (PI), Oakley B. (PI), B. Qurollo (Co-I). Improving and expanding the broad-range detection of canine tick-borne disease diagnostics using next-generation sequencing. Funded by the American Kennel Club Canine Health Foundation (2016- 2019) Role: Co-I Triangle Center for Evolutionary Medicine
B. Qurollo (PI), P. Larsen (Co-I), C. Williams (Co-I), E. Breitschwerdt (Co-I). Prevalence and Zoonotic Potential of Emerging Tick-borne Pathogens in Madagascar. Funded by TriCEM as Seed Funding for Research in Evolutionary Medicine (2016-2019). Role: PI Comparative Medicine Institute/Center for Gastrointestinal Biology and Disease Microbiome Pilot Project Initiative Qurollo (PI) and E. Breitschwerdt (Co-I). Next Generation Sequencing to Detect Novel Pathogens in the Blood of Dogs with Immune-mediated Hemolytic Anemia. Funded by the Comparative Medicine Institute/Center for Gastrointestinal Biology and Disease Microbiome Pilot Project Initiative (2016) Role: PI IDEXX Laboratories, Inc. Qurollo (PI) and E. Breitschwerdt (Co-I). Feline vector-borne disease in cats with acute onset fever (2016- 2018). Role: PI
OMB No. 0925-0001 and 0925-0002 (Rev. 09/17 Approved Through 03/31/2020)
BIOGRAPHICAL SKETCH
DO NOT EXCEED FIVE PAGES.
NAME: Ross Mathew Boyce
eRA COMMONS USER NAME (credential, e.g., agency login): ROSS_BOYCE
POSITION TITLE: Assistant Professor
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include
postdoctoral training and residency training if applicable. Add/delete rows as necessary.)
DEGREE
INSTITUTION AND LOCATION MM/YY FIELD OF STUDY
(if applicable)
Davidson College B.S. 05/01 Chemistry / Russian
London School of Hygiene & Tropical Medicine M.Sc. 03/12 Public Health
University of North Carolina at Chapel Hill (UNC) M.D. 05/12 Medicine
Massachusetts General Hospital (MGH) Residency 06/15 Internal Medicine
University of North Carolina at Chapel Hill (UNC) Fellowship 06/17 Infectious Diseases
A. Personal Statement
I am a physician scientist who uses epidemiological tools to identify, evaluate, and disseminate solutions to public
health problems, especially those related to infectious and vector-borne diseases. My areas expertise include tropical
medicine, clinical epidemiology, and spatial analysis, which I employ to advance our understanding of the geographic
factors that influence disease transmission. The underlying rationale behind my work is a desire to improve routine
disease surveillance and facilitate the implementation of evidence-based interventions in underserved areas.
Development and validation of robust diagnostic tools that can be deployed under sparse conditions to aid clinical
decision-making is a key component of this work. Overall, I believe that the leadership experience gained in the
military, together with formal training in infectious diseases and public health, provides me with a unique skillset to
serve as a mentor to a post-doctoral fellow undertaking One Health oriented research. I am invested and fully
committed to the success of the proposed program.
B. Positions and Honors
Positions and Employment
2002-2003 Rifle Platoon Leader, 2nd Infantry Division, US Army, Republic of Korea
2003-2004 Aide-de-Camp, 7th Infantry Division, US Army, Ft. Carson, CO
2004-2005 Reconnaissance Platoon Leader, 1st Cavalry Division, US Army, Baghdad, Iraq
2009-2010 Civil-Military Officer, 1st Cavalry Division, US Army, Mahmudiyah, Iraq
2012-2015 Resident, Internal Medicine, Massachusetts General Hospital, Boston, MA
Clinical Fellow in Medicine, Harvard Medical School, Boston, MA
2015-2017 Fellow, Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC
2017- Clinical Instructor, Division of Infectious Diseases, University of North Carolina at Chapel Hill
2018- Visiting Associate Professor, Department of Community Health, Mbarara University of Science
and Technology, Mbarara, Uganda
2018- Associate Editor, International Health
2019- Assistant Professor, University of North Carolina at Chapel Hill, Chapel Hill, NC
Other Experience and Professional Memberships
2012- Member, American Medical Association
2012- Member, American College of Physicians
2014- Member, American Society of Tropical Medicine and Hygiene
2015- Member, Infectious Disease Society of America2015- Diplomat, American Board of Internal Medicine: Certification in Internal Medicine
2017- Diplomat, American Board of Internal Medicine: Certification in Infectious Diseases
Honors
1996 Eagle Scout, Boy Scouts of America
2001 Phi Beta Kappa, Davidson College
2001 Magna Cum Laude and Honors in Chemistry, Davidson College
2001 Honor Graduate, United States Army Infantry School
2004 Bronze Star Medal with Valor Device for Heroism in Combat, United States Army
2006 Fullerton Foundation Scholarship, University of North Carolina at Chapel Hill School of Medicine
2012 Okeke Prize & William Simpson Prize, London School of Hygiene & Tropical Medicine
2012 Alpha Omega Alpha, University of North Carolina at Chapel Hill School of Medicine
2012 Merck Manual Award, University of North Carolina at Chapel Hill School of Medicine
2014 MGH Jackson Society White Coat Fund for Resident Research
2015 Outstanding Research Award, MGH Internal Medicine Residency Program
2015 Elsevier Clinical Research Award (3rd Place), ASTMH Annual Meeting
2016 First Prize, International Journal of Epidemiology Photo Essay Competition
2016 UNC Global Health Scholar
2017 ASTMH/Bill & Melinda Gates Annual Travel Meeting Award
2018 Best Clinical Article of the Year, American Journal of Tropical Medicine & Hygiene
C. Contributions to Science
1. Since first traveling to Western Uganda in 2012, I have been focused on the spatial epidemiology of malaria in this
highland area of great geographic diversity. With high mountain ridgelines nearing 2,000 meters in elevation only
a few kilometers from low-lying river valleys, the region is a microcosm of global malaria transmission. My efforts
to date have sought to better describe and explain the heterogeneity in transmission intensity across these
environments. A key component of this work is the use of geographic information systems (GIS), which I used to
create the first village-level map of the area. I am increasingly incorporating more powerful molecular measure of
transmission intensity with the intent of more accurately and effectively targeting control and elimination
interventions.
1. Boyce R, Brazeau N, Fulton T, et al. Prevalence of molecular markers of antimalarial drug resistance across
altitudinal transmission zones in highland Western Uganda . American Journal of Tropical Medicine and Hygiene.
2019; 101(4):799-802. doi: 10.4269/ajtmh.19-0081.
a. Boyce, R, Reyes, R, Mulogo, E, Ntaro, M, Matte, Metlay J, Band, L, Siedner, M. Severe flooding and
malaria transmission in a highland area of Uganda: Implications for disease control in an era of global
climate change. Journal Infectious Diseases. 2016: 214(9): 1403-1410. doi: 10.1093/infdis/jiw363.
b. Boyce R, Hathaway N, Fulton T, et al. Reuse of malaria rapid diagnostic tests for amplicon deep
sequencing to estimate Plasmodium falciparum transmission intensity in Western Uganda. Scientific
Reports. 2018. 8: 10159. doi:10:1038/s41598-018-28534-3.
2. My initial interest in malaria rapid diagnostic tests (RDT) was spurred by an observation, made to me by a
community health worker, that patients with both HRP-2 and pLDH antigen bands positive appeared more illthan those with only a single positive antigen band. This led me to explore possible biologic mechanisms
underlying the observation, eventually resulting in a small pilot study showing that patients with both antigen
bands positive had higher parasite density and more severe anemia. These findings provided the basis for the
larger, prospective Rapid Diagnostic Tests for Severe Malaria study, in which we attempted to use the
discriminatory ability of the multiple-antigen RDT to identify patients at high risk for severe malaria. The
overarching goal of this work is to improve the case management of malaria in high-transmission, rural settings
without access to more advanced diagnostic modalities.
a. Boyce, R, Reyes, R, Matte, M, Ntaro, M, Mulogo, E, Siedner, M. Use of a dual-antigen rapid diagnostic test to
screen children for severe P. falciparum malaria in a high-transmission, resource-limited setting.
Clinical Infectious Diseases. 2017: 65(9): 1509-1515. doi: 10.1093/cid/cix592
b. Murungi, M, Fulton, T, Reyes, R, Matte, M, Ntaro, M, Mulogo, E, Juliano, J, Siedner, M, Boum, Y, Boyce, R.
Improving the specificity of P. falciparum malaria diagnosis in high transmission settings with a two-step
RDT and microscopy algorithm. Journal of Clinical Microbiology. 2017; 55(5): 1540-1549. doi:
10.1128/JCM.00130-17.
c. Boyce, R, Muiru, A, Reyes, R, Mulogo, E, Ntaro, M, Matte, M, Siedner, M. Impact of rapid diagnostic tests
for the diagnosis and treatment of malaria at a peripheral health facility in Western Uganda: An
interrupted time series analysis. Malaria Journal. 2015: 14: 203.
3. During the World Health Organization’s most recent update of guidelines for the prevention and control of dengue
(2009), it became clear that the evidence base supporting vector control strategies was extremely limited. Led by
Dr. Olaf Horstick, we formed a collaboration seeking to consolidate existing studies through a series of systematic
reviews in order to highlight best practices and identify knowledge gaps. We are now approaching the conclusion
of this multi-year effort. I am transitioning to prospective, cross-sectional research to better define the
epidemiology of dengue in East Africa with particular interest in the potential misdiagnosis of dengue as malaria.
a. Horstick, O, Boyce, R, Runge-Ranzinger, S. Buidling the evidence base for dengue vector control:
searching for certainty in an uncertain world. Pathogens and Global Health. 2018: 112(8): 395-403.
b. Samuel, M, Maoz, D, Manrique, P, Ward, T, Ranzinger, S, Toledo, S, Boyce, R, Horstick, O. Community
Effectiveness of Indoor Residual Spraying as a Method: A Systemic Review. PLoS Negl Trop Dis. 2017:
11(8): e0005837. https://doi.org/10.1371/journal.pntd.0005837
c. Boyce, R, Lenhart, A, Kroeger, A, Velayudhan, R, Roberts, B, Horstick, O. Bacillus thurengiensis irsraelensis
(Bti) for the control of Dengue vectors: systemic literature review. Tropical Medicine & International
Health. 2013: 18(5): 564-577. doi:10.1111/tmi.12087
Complete list of published work in My Bibliography:
https://www.ncbi.nlm.nih.gov/sites/myncbi/1FS5fC8omYg51/bibliography/48276562/public/?sort=date&directi
on=ascending
D. Additional Information: Research Support and/or Scholastic Performance
Ongoing Research Support
Not assigned Boyce/Aiello (PI) 05/01/2019-04/30/2021
CDC/NC DHHS
Chatham County COVID Cohort (C4)
The goal of this longitudinal population-based cohort is to monitor the seroprevalence of SARS-CoV-2 infection
among a representative sample of residents in Chatham County.
Role: Co-PICOVID-19-2020-1 Hernandez (PI) 04/01/2020-09/1/2020 PCORI Healthcare Worker Exposure Response and Outcomes of Hydroxychloroquine Trial (HERO-HCQ) This multi-site, randomized controlled trial aims to test the effectiveness of hydroxychloroquine as prophylaxis against COVID-19 in a large cohort of frontline healthcare workers. Role: Site-PI K23 AI141764 Boyce (PI) 12/01/2018-11/31/2023 NIAID Serial killers to Mosquitos: The Spatial Targeting of Larval habitats in rural Uganda using geographic Profiling This study seeks to evaluate the use of geographic profiling, a novel spatial analysis tool derived from criminology, to more efficiently identify Anopheles larval habitats. Role: PI OPP1199232 Boyce (PI) 11/01/2018 – 04/30/2020 Gates Foundation Accessible metrics of access: Novel tools to measure immunization coverage The goal of the study is to assess three different metrics of healthcare access as surrogate measures of immunization coverage by comparing facility-based estimates of healthcare access to immunization rates as determined by a cross-sectional seroprevalence survey. Role: PI CFH Foundation Boyce (PI) 03/01/2018-12/30/2019 Getting malaria “off the back” of women and children in Uganda The aim of this pilot study is to determine the preliminary efficacy of applying insecticide to the cloth Ugandan mothers use to carry young children on their backs to reduce the incidence of malaria among infants. Role: PI Takeda Vaccines Boyce (PI) 07/01/2017 – 11/30/2019 Dengue as a cause of non-malarial febrile illness in Southwestern Uganda The aim of this study is to determine if the dengue virus is endemic in Southwestern Uganda by testing patients presenting with acute febrile illness with rapid diagnostic tests and PCR. Role: PI Completed Research Support 5T32AI007151-39 Margolis (PI) 07/01/2017 – 11/30/2018 NIAID Differing parasite populations in symptomatic and asymptomatic malaria To determine the genetic relatedness of P. falciparum parasite populations among children with symptomatic and asymptomatic malaria infection Role: Post-doctoral trainee Thrasher Research Fund Boyce (PI) 12/18/2014 – 12/30/2017 Early Career Award (13469) Rapid diagnostic tests for the identification of severe malaria The goal of this study is to explore the efficacy of a multiple antigen RDT as a low-cost, scalable marker of severe malaria for use in rural and peripheral health facilities Role: PI
OMB No. 0925-0001/0002 (Rev. 08/12 Approved Through 8/31/2015)
BIOGRAPHICAL SKETCH
Provide the following information for the Senior/key personnel and other significant contributors.
Follow this format for each person. DO NOT EXCEED FIVE PAGES.
NAME: Reiskind, Michael
eRA COMMONS USER NAME (agency login): MREISKIND
POSITION TITLE: Associate Professor
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as
nursing, include postdoctoral training and residency training if applicable.)
INSTITUTION AND LOCATION DEGREE Completion FIELD OF
(if applicable) Date STUDY
MM/YYYY
Amherst College, Amherst, MA AB 06/1995 Biology
University of Michigan, Ann Arbor, MI MPH 05/1999 Epidemiology
University of Michigan, Ann Arbor, MI PHD 05/2005 Biology
Florida Medical Entomology Laboratory, University Postdoctoral 08/2008 Mosquito
of Florida, Vero Beach, FL Fellow Biology
A. PERSONAL STATEMENT
I have the experience, training, and motivation to perform my role in the proposed research. I received
training in infectious disease epidemiology at the University of Michigan from 1997-1999, where I
completed thesis research on the epidemiology of dengue fever in the Amazonian region of Peru. This
research resulted in a publication that showed the importance of travel to urban centers as a risk factor for
dengue, and not peridomestic factors, an important discovery that other researchers have built upon. Since
that time, I have continued to focus my research on vector-borne disease, with a shift in focus to how
vector biology affects disease transmission in humans and animals. I have experience in several
mosquito-borne virus systems, including West Nile fever and chikungunya fever, in addition to dengue.
My research crosses several ecological scales, from individual physiology and behavior of mosquitoes to
landscape ecology of mosquito assemblages. I have also (starting in 2018), begun a research program on
the ecology of ticks as vectors of bacterial disease. My training and research experience are well suited to
the current proposal, as it will include a strong entomological component with a classic approach to the
epidemiology of a vector-borne disease. Publications pertinent to my abilities with regards to this
proposal:
a. Reiskind, M.O.B., Labadie, P.E., Bargielowski, I., Lounibos, L.P., and Reiskind, M.H. 2018.
Rapid evolution and the genomic consequences of selection against interspecific mating.
Molecular Ecology 27 (18): 3641-3654. Doi: https://doi.org/10.1111/mec.14821.Reiskind, M.H.
and Hopperstad, K.A. 2017. Gas stations for container breeding larval mosquito surveillance. J
Med Entomol. doi: https://doi.org/10.1093/jme/tjx129
b. Reiskind, M.H. and A.A. Zarrabi. 2012. Is bigger really bigger? Differential Responses to
Temperature in Measures of Body Size of the Mosquito, Aedes albopictus. J Insect Physiol. 58
(7): 911-917.
c. Reiskind, M.H. and Lounibos, L.P. 2009. Effects of intraspecific larval competition on adult
longevity in the mosquitoes Aedes aegypti and Aedes albopictus. Medical and Veterinary
Entomology 23: 62-68.B. POSITIONS AND HONORS
Positions and Employment
2008 - 2012 Assistant Professor, Department of Entomology and Plant Pathology, Oklahoma State
University, Stillwater, OK
2012 - Present Assistant Professor, Department of Entomology, North Carolina State University,
Raleigh, NC
Other Experience and Professional Memberships
2004 - Member, Society of Vector Ecology
2005 - Member, Entomological Society of America, Medical, Urban, and Veterinary Section
Officer (VP, P, PP), 2018-2022 (anticipated).
2005 - 2009 Member, Ecological Society of America
2008 - 2012 Editorial Board, Journal of Medical Entomology, Entomological Society of America
Honors
2011 Best Paper, 2009-2011, Medical and Veterinary Entomology, Royal Entomological
Society
2016 Singapore Infectious Disease Initiative Short-term Visitorship, Singapore (through
Duke-NUS Medical School)
2016 CALS Teaching Award of Merit
2017 Outstanding Publication, University Center for Atmospheric Research, Boulder, CO.
C. CONRIBUTIONS TO SCIENCE
1. In my career, I have focused on the ecology of infectious disease, primarily through the lens of
mosquito-borne pathogens. I have examined the host-arthropod-pathogen interaction from the perspective
of the mosquito, the most important arthropod vector of disease. As with any ecological system, this
interaction benefits from an analysis at multiple scales. As such, I have examined how the ecology of
mosquitoes has potential impacts on the vectorial capacity of mosquito populations. At a coarse scale, my
research is examining mosquito distribution across a landscape and understanding how that may change
the risk of disease. I have described what landscape factors determine what species of mosquitoes are
found where, a critical piece of information knowing where to focus control efforts. Examples of this
include:
a. Spence Beaulieu, M.R., Federico, J.L. and Reiskind, M.H. 2020. Mosquito diversity and dog
heartworm prevalence in suburban areas. Parasites and Vectors 13, 12.
https://doi.org/10.1186/s13071-019-3874-0
b. Monaghan, A.J., Morin, C.W., Steinhoff, D.F., Wilhelmi, O., Hayden, M., Quattrochi, D.A.,
Reiskind, M., Lloyd, A.L., Smith, K., Schmidt, C.A., Scalf, P.E., Ernst, K. 2016. On the
seasonal occurrence and abundance of the Zika virus vector Aedes aegypti in the contiguous
United States. PLoS Currents 8. doi:
10.1371/currents.outbreaks.50dfc7f46798675fc63e7d7da563da76.
c. Alto, B.W., Lounibos, L.P., Mores, C.N. and Reiskind, M.H. 2008. Larval competition alters
susceptibility of adult Aedes mosquitoes to dengue infection. Proceedings of the Royal Society
Biological Sciences 275: 463-471.
d. Cooper, V. S., Reiskind, M. H., Miller, J. A., Shelton, K. A., Walther, B. A., Elkinton, J. S. and
Ewald, P. W.. 2002. Timing of transmission and the evolution of virulence of an insect virus.
Proceedings of the Royal Society of London Series B-Biological Sciences 269:1161-1165.2. A second scale of inquiry to which I have made contributions is the interaction between species, with a
focus on competition between mosquito species, critical in determining the local/regional/global
distribution of vectors. Contributions in this area have been empirical and theoretical. I have also made a
unique contribution by illuminating the role of predation on adult mosquitoes with a study examining bats
and oviposition in Culex mosquitoes. These interactions can affect disease transmission either by altering
the landscape distribution (see previous) or by changing the vectorial capacity of a population of vectors.
a. Yee D.A., Himel E., Reiskind M.H., Vamosi S.M. 2014. Implications of saline concentrations for
the performance and competitive interactions of the mosquitoes Aedes aegypti (Stegomyia
aegypti) and Aedes albopictus (Stegomyia albopicta). Med Vet Entomol. 28(1):60-9. doi:
10.1111/mve.12007.. PubMed PMID: 23607885.
b. Reiskind M.H., Wund M.A.. Experimental assessment of the impacts of northern long-eared bats
on ovipositing Culex (Diptera: Culicidae) mosquitoes. J Med Entomol. 2009 Sep;46(5):1037-44.
PubMed PMID: 19769034; PubMed Central PMCID: PMC3749376.
c. Reiskind M.H., Wilson M.L. Interspecific competition between larval Culex restuans Theobald
and Culex pipiens L. (Diptera: Culicidae) in Michigan. J Med Entomol. 2008 Jan;45(1):20-7.
PubMed PMID: 18283938.
d. Vandermeer, J., Evans, M. A., Foster, P., Hook, T. Reiskind, M. and Wund, M. 2002. Increased
competition may promote species coexistence. Proceedings of the National Academy of Sciences
of the United States of America 99: 8731-8736.
3. Finally, I am interested in tying larval physiology and behavior to outcomes at the adult stage critical
for disease transmission. These include daily survival, oviposition behavior, and vector-competence.
These contributions include highly cited articles and provide important parameters for predictive
mathematical models. I also want to note that often my work transcends at least two distinct scales of
inquiry (see sections 1 and 2).
a. Spence-Beaulieu, M. and Reiskind, M.H. 2020. Comparative vector efficiency of two prevalent
mosquito species for dog heartworm in North Carolina. Journal of Medical Entomology, 57 (2),
608-614.
b. Reiskind, M.H., and Janairo, M.S. 2018. Tracking larval behavior across development: effects of
temperature and nutrients on individuals’ foraging behavior. Journal of Medical Entomology 55
(5): 1086-1092. https://doi.org/10.1093/jme/tjy073.
c. De Jesus, C.M. and Reiskind M.H. 2016. The importance of male body size on sperm uptake and
usage, and female fecundity in Aedes aegypti and Aedes albopictus. Parasites and Vectors 9:447.
Doi: 10.1186/s13071-016-1734-8.
d. Westbrook C.J., Reiskind M.H., Pesko K.N., Greene K.E., Lounibos L.P. Larval environmental
temperature and the susceptibility of Aedes albopictus Skuse (Diptera: Culicidae) to Chikungunya
virus. Vector Borne Zoonotic Dis. 2010 Apr;10(3):241-7. doi: 10.1089/vbz.2009.0035. PubMed
PMID: 19725768; PubMed Central PMCID: PMC2883477.
My bibliography from the NCBI is available
here: http://www.ncbi.nlm.nih.gov/sites/myncbi/1RiV8OBwveWA9/bibliograpahy/47426242/public/?sor
t=date&direction=ascendingD. RESEARCH SUPPORT
Completed Research Support
Faculty Research Development Program (internal). 2018-2019. “Testing Landscape Hypotheses of
Dengue Vector Mosquito Distributions.” $6,592. PI: M. Reiskind. Role: PI.
Inter-Institutional Planning Grant (UNC-GA). 2018-2019. “Establishing a State-Wide Public Health
Research Consortium: Addressing national entomological surveillance needs by
developing novel diagnostic methods through transdisciplinary collaborations.” $10,000.
PI: B. Byrd (WCU). Role: Co-PI
Department of Defense, Deployed Warfighters Protection Program, 2015-2019. “Kill-Pull-Kill: A New
Management Strategy for Filth Flies.” $749,109. PI: D.W. Watson, NCSU. Role: Co-PI.
Wake County, 2018-9. “Mosquito Surveillance Program for Wake County” $15,000. PI: M. Reiskind.
Role: PI.
North Carolina Department of Health and Human Services, 2018-9. “Mosquito-borne Disease Response
and Readiness for North Carolina.” $44,785. Role: PI.
North Carolina Department of Health and Human Services, 2018-9. “Tick Surveillance in North
Carolina.” $15,000. Role: PI.
North Carolina Department of Health and Human Services, 2018. “Investigations into North Carolina’s
Mosquito Threat.” $36, 916. Role: PI.
Wake County, 2018. “Mosquito Surveillance Program for Wake County ” $15,101. PI: M. Reiskind.
Role: PI.
North Carolina Department of Health and Human Services, 2017. “Mosquito Survey and Insecticide
Resistance in NC.” $54,844. PI: B. Byrd, Western Carolina University. Role: Co-PI.
North Carolina Department of Health and Human Services, 2017. “Vector-borne Disease Response.”
$30,000. PI: M. Reiskind. Role: PI.
North Carolina Department of Agriculture and Consumer Services, Pesticide Environmental Trust Fund,
2016-2017. “Regional Survey of Mosquito Control Knowledge and Usage in North
Carolina.” $6,625. PI: S. Richards, Eastern Carolina University. Role: Co-PI.
North Carolina Department of Health and Human Services, 2016. “Survey for Container-inhabiting
Aedes of Public Health Importance: North Carolina Preparedness Activities.”
$15,036.00. PI: B. Byrd, Western Carolina University. Role: Co-PI.
Wynne Innovation Grant, 2014-2015. “Development and application of a new genome sequencing
method for comparative genomics and gene mapping in non-model and ecologically
important species, the southern flounder (Paralichthys lethostigma) and the yellow fever
mosquito (Aedes aegypti). PI: M.O.B. Reiskind. Role: Co-PI. $40,000.
On-going Research Support
North Carolina Department of Health and Human Services, 2019-20. “Tick Surveillance for NC”
$70,000. Role: PI.
Wake County, 2019-20. “Mosquito Surveillance Program for Wake County” $15,000. PI: M. Reiskind.
Role: PI.
National Science Foundation, Systematics, Division of Environmental Biology, 2018-2021.
“Phylogenomics to Advance Understanding of World Mosquito Diversity and Vector
Ecology.” $1,190,595. PI: B. Wiegmann, NCSU. Role: Co-PI.OMB No. 0925-0001 and 0925-0002 (Rev. 09/17 Approved Through 03/31/2020)
BIOGRAPHICAL SKETCH
Provide the following information for the Senior/key personnel and other significant contributors.
Follow this format for each person. DO NOT EXCEED FIVE PAGES.
NAME: Bowman, Natalie McCarter
eRA COMMONS USER NAME: nbowman
POSITION TITLE: Assistant Professor of Medicine
EDUCATION/TRAINING
DEGREE Completion
(if Date FIELD OF STUDY
INSTITUTION AND LOCATION
applicable) MM/YYYY
Harvard College, Cambridge, MA AB 06/1999 Chemistry
Cambridge University, Cambridge, UK MPhil 11/2001 Biochemistry
Columbia University College of Physicians and MD 05/2007 Medicine
Surgeons, New York, NY
The Johns Hopkins Hospital, Baltimore, MD Residency 06/2010 Internal Medicine
University of North Carolina, Chapel Hill, NC Fellowship 06/2013 Infectious Diseases
UNC Gillings School of Global Public Health, MPH 08/2013 Epidemiology
Chapel Hill, NC
A. Personal Statement
I have diverse research and clinical experience in vector-borne infections, including Chagas disease, Zika, and
dengue. I have specific experience in the use of permethrin-impregnated clothing to protect from mosquito
bites, in a project where we demonstrated that permethrin-impregnated clothing maintained significant
repellant activity against Aedes aegypti mosquitoes despite genetic resistance to killing by pyrethroids. I also
have experience studying triatomines carrying At UNC, I have obtained a MPH in Epidemiology, which has
given me a strong methodological background in study design and data analysis. I have received funding from
the National Institutes of Health, Fogarty International Center, the UNC Center for AIDS Research (CFAR), and
the Burroughs Wellcome Fund/American Society of Tropical Medicine and Hygiene to support my research. I
am a practicing Infectious Diseases physician and routinely take care of vector-borne diseases in my patients. I
am currently working with the PI Dr. Boyce on another project examining the invasion of the Asian long-horned
tick into North Carolina, and I look forward to expanding our collaboration with this exciting new proposal.
B. Positions and Honors
Positions and Employment
1999-2000: Research assistant, Cardiology, Brigham and Women’s Hospital, Boston, MA
2004-2006: Research fellow, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, and AB
PRISMA, Lima, Peru
2007-2010: Internal medicine resident, Johns Hopkins Hospital, Baltimore, MD
2010-2011: Freelance writer, Johns Hopkins POC-IT Guide, Baltimore, MD
2010-2013: Infectious diseases fellowship, University of North Carolina at Chapel Hill, Chapel Hill, NC
2011-2013: Locum tenens, Moses Cone Healthcare, Greensboro, NC
2013: Locum tenens, Wake County Health Department HIV clinic, Raleigh, NC
2013-2014: Fogarty Global Health Fellow, University of North Carolina at Chapel Hill, Chapel Hill, NC
2013-2014: Clinical Instructor, University of North Carolina at Chapel Hill, Chapel Hill, NC
2014-present: Assistant Professor, University of North Carolina, Chapel Hill, Chapel Hill, NCLicensing and Certifications
2010-present: North Carolina Medical License #2010-01099
2010-present: American Board of Internal Medicine certification in Internal Medicine
2012-present: American Board of Internal Medicine certification in Infectious Diseases
Professional Memberships
2011-present: Member, Infectious Diseases Society of America
2011-present: Member, HIV Medicine Association
2012-present: Member, American Society of Tropical Medicine and Hygiene
Honors and Awards
1995-1999: Harvard College Scholarship
2000-2001: Cambridge Overseas Trust Bursary
2004-2005: Fogarty Ellison Overseas Fellowship in Global Health and Clinical Research
2006: Honorable mention, Alpha Omega Alpha Pharos Medical Student Essay Contest
2006: CDC Foundation OC Hubert Fellowship
2012: ID Week Fellows’ Day Workshop Travel Grant
2012: American Society of Tropical Medicine and Hygiene 2012 Annual Meeting Travel Award
2012-present: NIH Loan Repayment Program
2013: Delta Omega Honorary Society in Public Health
2013-2014: Fogarty Global Health Fellowship
2014-2016: UNC CFAR Developmental Award
2016: UNC Junior Faculty Development Award
2016: Caregivers at Carolina Travel Award
2017-present: Doris Duke Caregivers at Carolina Award
2019: Dr. James W. Woods Junior Faculty Award
C. Contributions to Science
1. Epidemiology and biology of disease vectors
I have been involved in projects characterizing the biology and ecology of insect vectors of Chagas disease
(Triatoma infestans) and flaviruses including dengue and Zika (Aedes aegypti). Studies I have led recently
have examined the phenotypic repellant effect of pyrethroids on pyrethroid-resistant mosquitoes and
characterized the hindgut microbiome of triatomines.
• Waltmann A, Willcox AC, Balasubramanian S, Borrini Mayor K, Mendoza Guerrero S, Salazar Sanchez
RS, Roach J, Condori Pino C, Gilman RH, Bern C, Juliano JJ, Meshnick SR, Levy MZ, Bowman NM.
Hindgut microbiota in laboratory-reared and wild Triatoma infestans. PLoS Neglected Tropical Diseases,
(2019) 13:e0007383. PMC6522061.
• Bowman NM, Akialis K, Cave G, Barrera R, Apperson C, Meshnick SR. Pyrethroid insecticides maintain
repellent effect on knockdown resistant populations of Aedes aegypti mosquitoes. PLoS One (2018)
13:e0196410. PMC5953453.
• Levy MZ, Bowman NM, Kawai V, Waller L, Cornejo del Carpio JG, Cordova Benzaquen E, Gilman RH,
Bern C. Peri-urban infestation by Triatoma infestans carrying Trypanosoma cruzi in Arequipa, Peru.
Emerging Infectious Diseases (2006) 12:1345-1352. PMC17073082.
2. Epidemiology, control, and pathogenesis of Zika virus infection
With collaborators in León, Nicaragua and at UNC, I participated in several projects studying the epidemiology
and pathogenesis of Zika virus infection during the American outbreak 2015-2017. My primary work involved a
longitudinal cohort of patients presenting with acute illness concerning for Zika virus. We followed these
patients for 6-12 months and measured viral shedding in various body fluids as well as examined risk factors
for infection. I was also involved in a pregnancy cohort and related birth cohort to examine neuropsychological
outcomes of Zika infection prior to birth or in early childhood.
• Becker-Dreps S, Stringer EM, Bucardo F, Bowman NM, Boivin MJ. Is there a silver lining to the American
Zika epidemic? Lancet Infectious Diseases (2020) 20:14-15• Kalmin M, Gower E, Stringer E, Bowman N, Rogawksi E, Westreich D. Misclassification in defining and
diagnosing microcephaly. Accepted to Paediatric and Perinatal Epidemiology (2019) 13:e0007383.
PMC6662617.
• *Reyes Y, *Bowman NM, Becker-Dreps S, Centeno E, Collins MH, Liou GJA, de Silva A, Bucardo F.
Prolonged shedding of Zika virus RNA in vaginal secretions from Nicaraguan women. Emerging Infectious
Diseases (2019) 25:808-810. PMC6433009.
• Collins MH, Tu HA, Gimblet-Ochieng C, Liou G-JA, Jadi RS, Metz SW, Thomas A, McElvany BD, Davidson
E, Doranz BJ, Reyes Y, Bowman NM, Becker-Dreps S, Bucardo F, Lazear HM, Diehl SA, de Silva AM.
The human antibody response to Zika targets type-specific quaternary structure epitopes. JCI Insight
(2019) 4:e124588. https://doi.org/10.1172/jci.insight.124588. PMC6538335.
3. The epidemiology of urban Chagas disease
This body of work describes the epidemiology of Chagas disease in periurban communities of Arequipa, Peru,.
In some neighborhoods, triatomines infested the majority of houses, but the presence of Trypanosoma cruzi
was more focalized. Our work showed that the parasite became established in a series of “micro-epidemics”
that occurred after widespread triatomine infestation in these neighborhoods. Since Chagas disease has
historically been a problem mainly in rural areas, these finding have important implications for control efforts,
especially vector control and human screening programs. Levy MZ, Small DS, Vilhena DA, Bowman NM,
Kawai V, Cornejo del Carpio JG, Cordova-Benzaquen EG, Gilman RH, Bern C, Plotkin J. Retracing micro-
epidemics of Chagas disease using epicenter regression. PLoS Computational Biology (2011) 7:e1002146.
PMC21935346.
• Levy MZ, Bowman NM, Kawai V, Plotkin JB, Waller LA, Cabrera L, Steurer F, Seitz AE, Pinedo-Cancino
VV, Cornejo del Carpio JG, Cordova-Benzaquen E, McKenzie FE, Maguire JH, Gilman RH, Bern C. Spatial
patterns in discordant diagnostic results for Chagas disease: links to transmission hotspots. Clinical
Infectious Diseases (2009) 26:134-145. PMC19278335.
• Bowman, NM, Kawai V, Levy MZ, Cornejo del Carpio JG, Cabrera L, Delgado F, Malaga F, Cordova
Benzaquen E, Pinedo VV, Steurer F, Seitz AE, Gilman RH, Bern C. Chagas disease transmission in
periurban communities of Arequipa, Peru. Clinical Infectious Diseases (2008) 46:1822-8. PMC18462104.
4. Epidemiology of Plasmodium falciparum malaria
I have worked on several projects investigating the epidemiology of Plasmodium falciparum malaria in Africa.
In the first, we found that while humoral immune responses to malaria were long-lived in Kenyan children, only
shorter-lived cellular responses were associated with protection from infection. In the second, we found that
malaria diversity in Lilongwe, Malawi was higher in children than in adults, perhaps due to poorly developed
immunity, and that there were differences in parasite populations locally. In the third, we analyzed a cohort of
pregnant women followed and discovered that HIV only increased risk of parasitemia in multigravidae.
• Bowman NM, Juliano JJ, Snider CJ, Kharabora O, Meshnick SR, Vulule J, John CC, Moormann AM.
Longevity of genotype-specific immune responses to Plasmodium falciparum merozoite surface protein 1 in
Kenyan children from regions of different malaria transmission intensity. American Journal of Tropical
Medicine and Hygiene (2016) 95:580-587. PMC5014263.
• Bowman NM, Congdon S, Mvalo T, Patel JC, Escamilla V, Emch M, Martinson F, Hoffman I, Meshnick
SR, Juliano JJ. Comparative population structure of Plasmodium falciparum circumsporozoite protein
NANP repeat lengths in Lilongwe, Malawi. Scientific Reports (2013) 3:1990. doi:10.1038/srep01990.
PMC23771124.
• Nhkoma ET, Bowman NM, Kalilani-Phiri L, Mwpasa V, Rogerson SJ, Meshnick SR. The effect of HIV
infection on the risk, frequency, and intensity of Plasmodium falciparum parasitemia in primigravid and
multigravida women in Malawi. American Journal of Tropical Medicine and Hygiene (2012) 87:1022-1027.
PMC23045249.
Online list of published work in MyBibliography:
https://www.ncbi.nlm.nih.gov/myncbi/natalie.bowman.1/bibliography/public/
D. Additional Information: Research Support
Ongoing Research SupportUniversity of North Carolina Bowman (PI) 04/03/2020-present Epidemiological and immunological aspects of Coronavirus disease 2019 (COVID-19) in North Carolina Role: Principal investigator The goal of this project is to characterize clinical features of COVID-19 in the North Carolina population and describe viral transmission through the population during the evolving SARS-CoV-2 epidemic. NC TraCS 550KR242003 Bowman (PI) 05/01/2020-04/30/2021 Epidemiological and immunological aspects of Coronavirus disease 2019 (COVID-19) in North Carolina Role: Principal investigator The goal of this project is to characterize clinical features of COVID-19 in the North Carolina population and describe viral transmission through the population during the evolving SARS-CoV-2 epidemic. University of North Carolina NC Collaboratory, NC DHHS Bowman (PI) 06/01/2020-12/31/2020 Tracking COVID-19 outbreaks in the food supply industry The goal of this project is to characterize transmission of SARS-CoV-2 in poultry, pork, and agricultural industries and to define factors that affect risk of transmission in the workplace and home. Role: Principal Investigator 2R01 AI107028 Gilman (PI) 07/01/2019-06/31/2024 Title: Predictors of cardiomyopathy progression in a Chagas disease cohort in Bolivia This study, a renewal of a cohort study examining biomarkers of Chagas cardiomyopathy, aims to more deeply examine the pathogenesis of Chagas cardiomyopathy using cardiac testing and transcriptomics to characterize immune and inflammatory pathways that play a role. Role: Coinvestigator R01 AI136722-01 Gilman (PI) 06/18/2018-05/31/2023 Title: Novel nanoparticular diagnostics for cerebral toxoplasmosis and Chagas in HIV patients living in Latin America The goal of this award is to develop new nanoparticle-based antigen detection tests using urine and/or cerebrospinal fluid to detect toxoplasmosis, Chagas disease, and tuberculosis in HIV-infected patients. Role: Coinvestigator R01 AI127845-01 Becker-Dreps (PI) 09/27/2016-08/31/2021 Natural history, immunity, and transmission patterns of sapovirus in a Nicaraguan birth cohort The goal of this award is to understand the epidemiology and natural history of sapovirus, characterize the development of immunity to sapovirus in early childhood, and describe patterns of transmission in households. Role: Coinvestigator R21 AI137902-01 Bowman, Becker-Dreps, Bucardo (co-PIs) 04/01/2019-03/31/2021 Title: Implications of congenital Zika virus infection This project evaluates the effect of maternal dengue immunity on risk of Zika virus infection during pregnancy, assesses the impact of congenital Zika virus exposure on primary dengue infection, and describes kinetics of Zika-specific maternally-acquired antibodies. Role: Lead Principal Investigator TriCEM Seed Grant Boyce (PI) 02/01/2020-01/31/2021 Title: Emergence of pyrethroid resistance in response to permethrin-treated uniforms This project has a goal to evaluate the ecological effect of the use of permethrin-impregnated uniforms on the prevalence of insecticide resistance in local mosquito populations. UNC Explorations in Global Health Bowman (PI) 08/01/2019-12/01/2020 Title: The use of novel point-of-care diagnostics for toxoplasmosis and Chagas disease in people living with HIV in Peru and Bolivia The goal of this project is to develop recombinase polymerase amplification-lateral flow assays to detect these pathogens in the blood and cerebrospinal fluid of HIV-infected patients and to transfer this knowledge to laboratories at our sites in Peru and Bolivia.
BIOGRAPHICAL SKETCH
Provide the following information for the Senior/key personnel and other significant contributors.
Follow this format for each person. DO NOT EXCEED FIVE PAGES.
NAME: : Edward B. Breitschwerdt
eRA COMMONS USER NAME (credential, e.g., agency login): ebbreits
POSITION TITLE: Professor of Medicine and Infectious Diseases
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing,
include postdoctoral training and residency training if applicable. Add/delete rows as necessary.)
Completion
DEGREE
Date FIELD OF STUDY
INSTITUTION AND LOCATION (if applicable)
MM/YYYY
University of Maryland, College Park, MD BS 1970 Animal Science
University of Georgia, College of Veterinary Medicine, DVM 1974 Veterinary Medicine
Athens, GA
Internship 1975 Board Certification,
University of Missouri, Columbia MO Medicine 1977 Veterinary Internal
Residency Medicine
A. Personal Statement
I have spent my career as an academic veterinary internist with a research interest in vector-transmitted
intracellular pathogens. For over 25 years, I have been the co-director of the NCSU-CVM Vector-Borne
Diseases Diagnostic Laboratory, principal investigator for the Intracellular Pathogens Research Laboratory and
more recently, Director of the NCSU Biosafety Level III Laboratory in the NCSU Comparative Medicine
Institute. Historically, our research has focused on important tick-transmitted pathogens in the genera
Anaplasma, Babesia, Borrelia, Ehrlichia and Rickettsia.
For the past decade, we have emphasized studies directed at the genus Bartonella. From a comparative
medical perspective, Bartonella are critically important emerging pathogens in both human and veterinary
medicine. Most recently, my research group has lead efforts to enhance the diagnostic detection of these
highly fastidious bacteria in animal and human patient samples. As a result, we have generated preliminary
case-based data to support chronic intravascular infection with several Bartonella spp. in immunocompetent
humans. We have also generated data to support a potential role for persistent Bartonella spp. infection as a
cause of cardiovascular, neurologic and rheumatologic abnormalities in human beings.
1. Breitschwerdt EB, RG Maggi, WL Nicholson, NA Cherry, CW Woods: Bartonella spp. bacteremia in
patients with neurological and neuro-cognitive dysfunction. J Clin Microbiol 46:2856-2861; 2008.
2. Maggi RG, PE Mascarelli, EL Pultorak, BC Hegarty, JM Bradley, BR Mozayeni, EB Breitschwerdt: Bartonella
spp. bacteremia in high-risk immunocompetent patients. Diagn Microbiol Infect Dis. 71: 43-37; 2011.
3. Yancey CB, Hegarty BC, Qurollo BA, Levy MG, Birkenheuer AJ, Weber DJ, Diniz PPVP, Breitschwerdt EB.
Regional seroreactivity and vector-borne disease co-exposures in dogs in the United States from 2004-2010:
Utility of canine surveillance. Vector Borne Zoonotic Dis 14:724-732; 2014.
B. Positions and Honors
1982–present Associate/Professor: Medicine and Infectious Diseases, North Carolina State University College
of Veterinary Medicine, Raleigh, NCYou can also read
