Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics

 
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Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics
Master thesis topics 2019-2020

                        Department of Biology

Laboratory of Biodiversity and Evolutionary Genomics
                                    Prof. Flip Volckaert
Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics
The VOLCKAERT
                Team

                                    Ecology        Evolution

Fundamental research at the interface of
        ecology & evolution

with applications in fisheries, aquaculture, human biology
                 and conservation biology
Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics
Topics for master
     theses

              Trophic ecology
                of Arctic fish

  Adaptation to pollution
    in freshwater fish
                     Parasitology of
                     freshwater fish
Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics
Topics Master in Biology

The following five topics can be selected by students
following the English taught Master in Biology or Dutch
taught Master Biologie.
We have also two topics for students in the Master of
Sustainable Development.
Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics
Patterns of parasite diversity in three riverine
                                     Title
                              fishes – I and II

Promoter        Prof. Dr. Filip Volckaert (Filip.Volckaert@bio.kuleuven.be, 016 32 39 72)
Supervisor      Io Deflem (io.deflem@kuleuven.be)
Aim
We’ll study patterns of parasite communities and distribution in three fish
species. Previous studies have shown that parasite diversity and
abundance differ drastically among populations. We will investigate which
factors influence these differences. Low water quality and pollution are
well-known problems in Flemish rivers and affect fish communities in many
ways but do they also influence fish parasite communities? Or does host
diversity play an important role? We will compare the impact between
three fish species: three-spined stickleback, a very tolerant and robust
species, stone loach, a bottom-dwelling and sensitive species and
topmouth gudgeon, a highly tolerant and invasive species, all from the
Demer basin. Practical work will focus on dissecting fish and screening them
for the presence of ecto- and endoparasites. This project is highly flexible
and can be split into multiple subprojects; we aim at two students.
Application
The research fits in a project with the Flemish Environmental Agency to
improve river management.
Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics
Patterns of parasite diversity in three riverine
                                         fishes – I and II
                                                                             Stone loach, three-spined
Sampling locations in the Dijle-Demer basin         Sampling                 stickleback, topmouth
                                                                              gudgeon
Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics
IRO            Evolutionary ecology –
                            Life history traits of Tanganyika sardines:
                           providing data for sustainable management
Promoter     Prof. dr. Filip Volckaert
             filip.volckaert@kuleuven.be, tel: 016 32 39 72

Supervisor   Els De Keyzer
             els,dekeyzer@@kuleuven.be
                                                              Aim
                                   You will work with samples of sardines of Lake
                                   Tanganyika in order to understand more of their
                                   population structure and life cycle. This way you help
                                   in providing much needed information to increase
                                   sustainability of the LT fisheries.
                                   You will do lab work, including (geo)morphometrics,
                                   and will be responsible for data collection and
                                   analysis. If the opportunity arises, the project may
                                   involve fieldwork in DR Congo (pending a successful
                                   application for an IRO grant).

                              Application
     The fisheries of Lake Tanganyika are an important food
     source for millions of people living near the lake shores.
     Almost 60% of the catch consists of two sardine species,
     and these populations now threaten to collapse. To
     enable effective management, more information about
     the biology of the two sardine species is much needed.
Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics
Evolutionary ecology –
                       Life history traits of Tanganyika sardines:
                      providing data for sustainable management

Relevant for management of                                      Two endemic pelagic clupeids make
an essential food source                                        up most of the fisheries catch

                                                                                   Stolothrissa tanganicae

                                                                                        Limnothrissa miodon

                                                                    Tanganyika, one
                                                                    of the oldest and
                                                                    largest lakes in
                               (geo)Morphometrics to discover       the world
                                 hidden population structure
Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics
Adaptation of the three-spined stickleback
                                   to heavy metals

Promoter                      Prof. Dr. Filip Volckaert
(Filip.Volckaert@bio.kuleuven.be, 016 32 39 72)

Supervisor                    Anna Kordas (anna.kordas@kuleuven.be, 016 19 42 93)

                                       Aim
                                       Heavy metal pollution in the aquatic environment remains a
                                       major concern. Fish may survive at sites where toxic substances
                                       exceed lethal levels. So far we have established that certain
                                       populations do seem to survive at higher levels of mercury in the
                                       Scheldt and Meuse basin. To understand if this happens due to
                                       acclimation or adaptation processes, an experiment involving
                                       multi-generational exposure to mercury (Hg) will be performed.
                                       Parents are crossed and their sibs raised in aquaria. The progeny
                                       are exposed to various concentrations of mercury and various
                                       biological end points measured. The findings will help to
                                       understand the molecular and genetic mechanisms. Model
                                       organism is the freshwater three-spined stickleback (TSSB).

                                       Application
                                       The results of your thesis will give a better assessment of the risks
                                       our freshwater ecosystems are facing.
Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics
Adaptation of the three-spined stickleback
                                  Title
                          to heavy metals

                       This project has three components

                                      http://carinbondar.com/wp-
                         content/uploads/2010/04/stickleback.jpg

                                                                   Challenge tests of juveniles
                              Lab work: breeding
Field work                                                         exposed to mercury
                              of F1 generation
Evolutionary
                 Evolutionary ecology – ecology –
                                        Metabarcoding   of
                       Metabarcoding
                polar fish: microbiome ofcomposition
                                          polar fish: in a
               microbiome coposition
                              changing in a changing Arctic
                                        Arctic
Promoter         Prof. Dr. Filip Volckaert (filip.volckaert@bio.kuleuven.be, 016 32 39 72)
Supervisor       Sarah Maes (sarah.maes@kuleuven,be)
Aim
Climate-induced changes put an increasing pressure on the Arctic ecosystem and
its populations, including the abundant circumpolar fish polar cod (Boreogadus
saida). Changes in the abundance and distribution of this keystone species will
impact the entire Arctic food web. Therefore, good knowledge of polar cod’s
genetics and ecology is essential. Gut microbiota of fish can be sensitive to dietary
changes. Moreover, microbiota diversity and composition can vary among
populations. In this project, we aim to investigate microbiome composition of polar
cod and the link between diet and microbiome.

Application
Next to climate change, also global fisheries are
expanding towards the poles and put increasing pressure
on the ecosystems Solid understanding of local
populations is needed to advance conservation
measures and/or sensible management.
Evolutionary ecology – Metabarcoding of
                   polar fish: microbiome composition in a
                                changing Arctic
This project involves molecular laboratory techniques (DNA extraction, PCR, gel
electrophoresis) and statistical examination of the gut microbiome composition.

     Boreogadus saida

This is a great opportunity for students with interest in marine polar biology & genetics!
Good to remember
Our research involves :
- field sampling                                  Trophic ecology
                                                    of Arctic fish
-   lab work
-   collaborative research
-   excellent tutoring
                                     Adaptation to pollution
-   international contacts             in freshwater fish
-   societal applications                               Parasitology of
                                                        freshwater fish

For information consult bio.kuleuven.be/eeb/lbeg and
   https://wet.kuleuven.be/apps/thesis
Please drop by at our lab for more information!

                                               http://bio.kuleuven.be/eeb/lbeg
Conservation Genetics –
                       Conservation genetics of the Edwards’s
                           pheasant (Lophura edwardsi)
 Promoter           Prof. Dr. Filip Volckaert (filip.volckaert@bio.kuleuven.be, 016 32 39 72)
 Co-promoter Dr. Peter Galbusera (Peter.Galbusera@kmda.org

 Aim
 The pheasant Lophura edwardsi lives in the forests of central Vietnam and is listed Critically
 Endangered. The species might be even closer to extinction and more difficult to rescue than
 previously thought. Fortunately, representatives of the species are still present in a captive stock
 that was set up between 1924 and 1930 and is currently housed in both private collections and at
 zoological gardens, with no extra influx from the wild. This population has been proposed to serve
 as a source to rescue the species in the wild. Unfortunately the population is derived from 28
 individuals of which only 6 to 8 females. Previous mitochondrial DNA analyses showed that
 genetic variability within this captive population has seriously declined, most likely due to a severe
 bottleneck that occurred between 1942 and 1947. Since analyses have been based on
 mitochondrial or maternally inherited DNA, some hybrid birds might have been left undetected.
 However, after several back-crosses with pure birds, the chance to discover a hybrid becomes
 more and more difficult. Hence a sufficient number of nuclear (microsatellite) markers has to be
 used to separate pure from hybrid L. edwardsi. The student will generate detailed insights in the
 (relative) amount, spread and optimal management of genetic diversity in the captive
 population. Historical samples stored in musea will serve as a reference for the wild population, a
 reference that will be of use to further verify for hybridization within this population.

Application
Your results support the conservation of biodiversity and the forest in Central Vietnam.
Conservation Genetics –
                 Conservation genetics of the Edwards’s
                     pheasant (Lophura edwardsi)

This project involves literature and database research, sample prepation, genotyping
and computational work. Knowledge of numerical computing in R is appreciated.

It is a great opportunity for students with an interest in the management of tropical
fowl. Working place will be Leuven with regular visits to the zoo in Antwerp.
Evolutionary ecology – Global Minds
                   Hybridization of Schistosoma species in Zimbabwe
                  and South Africa: Implications for animal and human
                                  disease transmission

                            Co-promoter: Dr. Tine Huyse (tine.huyse@kuleuven.be, 02 769 57 63)
              Promoter: Prof. Dr. Filip Volckaert (filip.volckaert@kuleuven.be, 016 32 39 72)

    Aim: Man-made lakes are especially prone to the introduction of non-indigenous species,
              due to their young age and high niche availability. When these species become
           invasive, they can destabilize the ecosystem. Lake Kariba is one of the largest man-
          made lakes in the world, but studies on non-indigenous species are scarce. Here we
      focus on invasive freshwater molluscs that can host trematode parasites, which are the
             causative agent of fasciolosis and other snailborne diseases affecting wildlife and
         livestock. The following tasks are planned. (1) 6 weeks of fieldwork, collection of snail
       hosts and stool samples from putative wildlife hosts (Zimbabwe)and (2) literature study,
      PCR analysis to identify parasite infection, data analysis and reporting at Royal Museum
                                                    for Central Africa and KU Leuven (Belgium).
Justification: This study will allow to identify the role of invasive snail species in the
      epidemiology of infectious diseases and to quantify the infection pressure for wildlife in
      Kariba.
Evolutionary ecology – IRO
                     Hybridization of Schistosoma species in Zimbabwe
                    and South Africa: Implications for animal and human
                                    disease transmission.

Life cycle Schistosoma   Schistosome parasite    Bulinus snails           shedding experiments
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