Master thesis topics 2019-2020 - Department of Biology Laboratory of Biodiversity and Evolutionary Genomics
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Master thesis topics 2019-2020 Department of Biology Laboratory of Biodiversity and Evolutionary Genomics Prof. Flip Volckaert
The VOLCKAERT Team Ecology Evolution Fundamental research at the interface of ecology & evolution with applications in fisheries, aquaculture, human biology and conservation biology
Topics for master theses Trophic ecology of Arctic fish Adaptation to pollution in freshwater fish Parasitology of freshwater fish
Topics Master in Biology The following five topics can be selected by students following the English taught Master in Biology or Dutch taught Master Biologie. We have also two topics for students in the Master of Sustainable Development.
Patterns of parasite diversity in three riverine Title fishes – I and II Promoter Prof. Dr. Filip Volckaert (Filip.Volckaert@bio.kuleuven.be, 016 32 39 72) Supervisor Io Deflem (io.deflem@kuleuven.be) Aim We’ll study patterns of parasite communities and distribution in three fish species. Previous studies have shown that parasite diversity and abundance differ drastically among populations. We will investigate which factors influence these differences. Low water quality and pollution are well-known problems in Flemish rivers and affect fish communities in many ways but do they also influence fish parasite communities? Or does host diversity play an important role? We will compare the impact between three fish species: three-spined stickleback, a very tolerant and robust species, stone loach, a bottom-dwelling and sensitive species and topmouth gudgeon, a highly tolerant and invasive species, all from the Demer basin. Practical work will focus on dissecting fish and screening them for the presence of ecto- and endoparasites. This project is highly flexible and can be split into multiple subprojects; we aim at two students. Application The research fits in a project with the Flemish Environmental Agency to improve river management.
Patterns of parasite diversity in three riverine fishes – I and II Stone loach, three-spined Sampling locations in the Dijle-Demer basin Sampling stickleback, topmouth gudgeon
IRO Evolutionary ecology – Life history traits of Tanganyika sardines: providing data for sustainable management Promoter Prof. dr. Filip Volckaert filip.volckaert@kuleuven.be, tel: 016 32 39 72 Supervisor Els De Keyzer els,dekeyzer@@kuleuven.be Aim You will work with samples of sardines of Lake Tanganyika in order to understand more of their population structure and life cycle. This way you help in providing much needed information to increase sustainability of the LT fisheries. You will do lab work, including (geo)morphometrics, and will be responsible for data collection and analysis. If the opportunity arises, the project may involve fieldwork in DR Congo (pending a successful application for an IRO grant). Application The fisheries of Lake Tanganyika are an important food source for millions of people living near the lake shores. Almost 60% of the catch consists of two sardine species, and these populations now threaten to collapse. To enable effective management, more information about the biology of the two sardine species is much needed.
Evolutionary ecology – Life history traits of Tanganyika sardines: providing data for sustainable management Relevant for management of Two endemic pelagic clupeids make an essential food source up most of the fisheries catch Stolothrissa tanganicae Limnothrissa miodon Tanganyika, one of the oldest and largest lakes in (geo)Morphometrics to discover the world hidden population structure
Adaptation of the three-spined stickleback to heavy metals Promoter Prof. Dr. Filip Volckaert (Filip.Volckaert@bio.kuleuven.be, 016 32 39 72) Supervisor Anna Kordas (anna.kordas@kuleuven.be, 016 19 42 93) Aim Heavy metal pollution in the aquatic environment remains a major concern. Fish may survive at sites where toxic substances exceed lethal levels. So far we have established that certain populations do seem to survive at higher levels of mercury in the Scheldt and Meuse basin. To understand if this happens due to acclimation or adaptation processes, an experiment involving multi-generational exposure to mercury (Hg) will be performed. Parents are crossed and their sibs raised in aquaria. The progeny are exposed to various concentrations of mercury and various biological end points measured. The findings will help to understand the molecular and genetic mechanisms. Model organism is the freshwater three-spined stickleback (TSSB). Application The results of your thesis will give a better assessment of the risks our freshwater ecosystems are facing.
Adaptation of the three-spined stickleback Title to heavy metals This project has three components http://carinbondar.com/wp- content/uploads/2010/04/stickleback.jpg Challenge tests of juveniles Lab work: breeding Field work exposed to mercury of F1 generation
Evolutionary Evolutionary ecology – ecology – Metabarcoding of Metabarcoding polar fish: microbiome ofcomposition polar fish: in a microbiome coposition changing in a changing Arctic Arctic Promoter Prof. Dr. Filip Volckaert (filip.volckaert@bio.kuleuven.be, 016 32 39 72) Supervisor Sarah Maes (sarah.maes@kuleuven,be) Aim Climate-induced changes put an increasing pressure on the Arctic ecosystem and its populations, including the abundant circumpolar fish polar cod (Boreogadus saida). Changes in the abundance and distribution of this keystone species will impact the entire Arctic food web. Therefore, good knowledge of polar cod’s genetics and ecology is essential. Gut microbiota of fish can be sensitive to dietary changes. Moreover, microbiota diversity and composition can vary among populations. In this project, we aim to investigate microbiome composition of polar cod and the link between diet and microbiome. Application Next to climate change, also global fisheries are expanding towards the poles and put increasing pressure on the ecosystems Solid understanding of local populations is needed to advance conservation measures and/or sensible management.
Evolutionary ecology – Metabarcoding of polar fish: microbiome composition in a changing Arctic This project involves molecular laboratory techniques (DNA extraction, PCR, gel electrophoresis) and statistical examination of the gut microbiome composition. Boreogadus saida This is a great opportunity for students with interest in marine polar biology & genetics!
Good to remember Our research involves : - field sampling Trophic ecology of Arctic fish - lab work - collaborative research - excellent tutoring Adaptation to pollution - international contacts in freshwater fish - societal applications Parasitology of freshwater fish For information consult bio.kuleuven.be/eeb/lbeg and https://wet.kuleuven.be/apps/thesis Please drop by at our lab for more information! http://bio.kuleuven.be/eeb/lbeg
Conservation Genetics – Conservation genetics of the Edwards’s pheasant (Lophura edwardsi) Promoter Prof. Dr. Filip Volckaert (filip.volckaert@bio.kuleuven.be, 016 32 39 72) Co-promoter Dr. Peter Galbusera (Peter.Galbusera@kmda.org Aim The pheasant Lophura edwardsi lives in the forests of central Vietnam and is listed Critically Endangered. The species might be even closer to extinction and more difficult to rescue than previously thought. Fortunately, representatives of the species are still present in a captive stock that was set up between 1924 and 1930 and is currently housed in both private collections and at zoological gardens, with no extra influx from the wild. This population has been proposed to serve as a source to rescue the species in the wild. Unfortunately the population is derived from 28 individuals of which only 6 to 8 females. Previous mitochondrial DNA analyses showed that genetic variability within this captive population has seriously declined, most likely due to a severe bottleneck that occurred between 1942 and 1947. Since analyses have been based on mitochondrial or maternally inherited DNA, some hybrid birds might have been left undetected. However, after several back-crosses with pure birds, the chance to discover a hybrid becomes more and more difficult. Hence a sufficient number of nuclear (microsatellite) markers has to be used to separate pure from hybrid L. edwardsi. The student will generate detailed insights in the (relative) amount, spread and optimal management of genetic diversity in the captive population. Historical samples stored in musea will serve as a reference for the wild population, a reference that will be of use to further verify for hybridization within this population. Application Your results support the conservation of biodiversity and the forest in Central Vietnam.
Conservation Genetics – Conservation genetics of the Edwards’s pheasant (Lophura edwardsi) This project involves literature and database research, sample prepation, genotyping and computational work. Knowledge of numerical computing in R is appreciated. It is a great opportunity for students with an interest in the management of tropical fowl. Working place will be Leuven with regular visits to the zoo in Antwerp.
Evolutionary ecology – Global Minds Hybridization of Schistosoma species in Zimbabwe and South Africa: Implications for animal and human disease transmission Co-promoter: Dr. Tine Huyse (tine.huyse@kuleuven.be, 02 769 57 63) Promoter: Prof. Dr. Filip Volckaert (filip.volckaert@kuleuven.be, 016 32 39 72) Aim: Man-made lakes are especially prone to the introduction of non-indigenous species, due to their young age and high niche availability. When these species become invasive, they can destabilize the ecosystem. Lake Kariba is one of the largest man- made lakes in the world, but studies on non-indigenous species are scarce. Here we focus on invasive freshwater molluscs that can host trematode parasites, which are the causative agent of fasciolosis and other snailborne diseases affecting wildlife and livestock. The following tasks are planned. (1) 6 weeks of fieldwork, collection of snail hosts and stool samples from putative wildlife hosts (Zimbabwe)and (2) literature study, PCR analysis to identify parasite infection, data analysis and reporting at Royal Museum for Central Africa and KU Leuven (Belgium). Justification: This study will allow to identify the role of invasive snail species in the epidemiology of infectious diseases and to quantify the infection pressure for wildlife in Kariba.
Evolutionary ecology – IRO Hybridization of Schistosoma species in Zimbabwe and South Africa: Implications for animal and human disease transmission. Life cycle Schistosoma Schistosome parasite Bulinus snails shedding experiments
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