Investigation of Microplastic Contamination in Vietnamese Sea Salts Based on Raman and Fourier-Transform Infrared Spectroscopies

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Investigation of Microplastic Contamination in Vietnamese Sea Salts Based on Raman and Fourier-Transform Infrared Spectroscopies
EnvironmentAsia 14(2) 2021 1-13
                                                                                DOI 10.14456/ea.2021.11
                                                               ISSN 1906-1714; ONLINE ISSN: 2586-8861

  Investigation of Microplastic Contamination in Vietnamese
  Sea Salts Based on Raman and Fourier-Transform Infrared
                        Spectroscopies
     Vo Thi Kim Khuyen1*, Dinh Vu Le2*, Le Hung Anh3, Axel René Fischer1
                           and Christina Dornack1
 1
  Institut für Abfall- und Kreislaufwirtschaft, Technische Universität Dresden, Pirna, Germany
          2
           Faculty of Chemical Engineering, Industrial University of Ho-Chi-Minh City,
                                     Ho-Chi-Minh City, Vietnam
               3
                Institute for Environmental Science, Engineering and Management,
              Industrial University of Ho-Chi-Minh City, Ho-Chi-Minh City, Vietnam
          *Corresponding author: kimkhuyenvo@gmail.com, ledinhvu@iuh.edu.vn
     Received: January 14, 2021; Revised: February 26, 2021; Accepted: March 28, 2021

Abstract
Microplastics (MPs) are plastic items smaller than 5 mm as a result of the degradation of
plastic products and wastes in the environment. They have been encountered in a variety of
sea products, including sea salts. The aim of this research is to investigate the microplastic
contamination in Vietnamese sea salts based on Raman and Fourier-Transform infrared
(FT-IR) spectroscopies. A total of 14 salt brands and no-name salt bags collected randomly at
six urban and suburban groceries in Ho-Chi-Minh city were analyzed. The results showed the
presence of polymers in the decreasing percentages - PET, PE, PP, PVC, PS, PA-6, and PMMA.
there was a higher fluctuation in MPs amount in non-branded salts compared with branded
salts. However, the average of MPs was fairly similar – 133.62 items/kg, corresponding to
487.71 items entering the human body per year via salt consumption. All these obtained results
revealed MPs pollution in Vietnam sea salts may come from seawater. This study supplied more
information about MPs pollution in the sea and for environmental research and food safety.

Keywords: Anthropogenic microfibers; Sea salt contamination; Raman and Fourier-Transform
infrared spectroscopies; Can Gio Vietnam

1. Introduction
     Plastic pollution has been one of the             a result of physical, chemical and biological
major global environmental concerns due to             fragmentation of larger plastics (Urbanek
the triple increase in amount of the global            et al., 2018). The later form can be seen in
plastic waste (Lebreton and Andrady, 2019).            a heterogeneous mixture of diverse shaped
Vietnam is in world-top 5 countries with the           plastic materials such as pellets, fragments,
estimated amount of plastic waste discharged           fibers and other shapes in the range of
into the sea from 0.28 to 0.73 million tons/year       0.1 – 5000 µm (Hidalgo-Ruz et al., 2012).
(Le, 2019). Plastic waste experienced                  They have a variety of colors and shapes
degradation in silent ways in the environment          commonly particles and fibers depending
by breaking into tiny pieces called micro              on their sources and relevant degradations
plastics (MPs). They are classified into               (Rezania et al., 2018). Light micro debris
primary meso plastics (
Investigation of Microplastic Contamination in Vietnamese Sea Salts Based on Raman and Fourier-Transform Infrared Spectroscopies
V. T. K. Khuyen et al / EnvironmentAsia 14(2) (2021) 1-13

away from pollution sources (Urbanek et al.,         Sever studies have reported the presence of
2018). Hence, an exact identification of micro       MPs in 94% of salts tested worldwide (Lee
plastic pollution sources and their behaviors        et al., 2019). The MPs abundance in table salts
is still an extreme challenge.                       varied with countries, from 9.7 MPs/kg (PP,
     Although MPs have been found in                 PE, PET) in Taiwan salts (Lee et al., 2019),
various types of samples, it is difficult to         56 – 103 MPs/kg (polyesters, PET, PP, PA, PE
compare their abundance exactly owing to             and PS) in Indian salts (Seth and Shriwastav,
no standardized MPs analysis procedures at           2018) to 50 – 280 MPs/kg (mostly PE and PP)
this moment. A typical workflow comprises            in Spain (Iñiguez et al., 2017).
physical and spectroscopic qualification. The             There are three salt types on Vietnamese
anthropogenic debris could be directly seen          market: crystalized salt coarse, fine salt refined
under a stereomicroscope, or a dissection            by companies, and iodized salt. An average of
microscope after stained with Rose Bengal            5 g per day for a healthy adult is recommended
(Kosuth et al., 2018), or a fluorescence             by The World Health Organization (Peixoto
microscope after incubated with fluorescent          et al., 2019), and MPs entered in the human
dye Nile Red. Although Nile Red method               body with a large amount, (0 – 7.3) x 104 items
is highly sensitive, biogenic materials such         annually via salt consumption (Zhang et al.,
as lipids and chitin creating fluorescent            2020). The trend in salty food consumption
signals will interfere the plastic detection         is increasing in several nations including
(Erni-Cassola et al., 2017). Thus, this              Vietnam with up to 10 g/day (Jensen
method is preferable for environmental               et al., 2018) because salts are the main
than biota samples. In the meanwhile,                component in almost Vietnamese kinds
the Fourier Transform infrared (FT-IR)               of spices. Though, the research of MPs in
spectroscopies have become a conventional            Vietnamese marine salts is still very rare.
tool to determine MPs in bivalves, honey             Up to now, only study of Kim et al. (2018)
and sugar, tap water and beer (Kosuth et             used FT-IR to detect MPs in two salt brands
al., 2018), drinking water, table salt and air       of Vietnam. Focus on these points, this
(Zhang et al., 2020). Recently, Raman has            study aims to quantify the MPs in all salt
attracted more attention in the literature due       types of branded and non-branded salts in
to more advantages than FT-IR. Raman is              Ho-Chi-Minh city by Raman microscope
considered to replace for FT-IR thanks to            and FT-IR for further prediction of MPs
better resolution, wider spectral coverage           accumulation in the human body via salt
and lower water interference (Ribeiro - Claro        digestion.
et al., 2017), and was employed to identify
16 – 84 items/kg MPs in table salt of Turkey         2. Materials and Methods
(Gündoğdu, 2018) and other countries
(Karami et al., 2017).                               2.1 Sampling
     There are no salt mines in Vietnam
and the salt manufacture relies on water                  A total of 14 brands were purchased
evaporation and salt crystallization from            from urban and suburban markets of Ho-Chi
seawater under the prolonged exposure to             Minh city. The commercial names are kept
strong sunlight, favorable wind and moisture         confidential and marked by alphabetically
on thousands of hectares of open ponds               letters. Branded products are labeled with
along with coastal provinces from Northern,          ingredients, production place and expiration
Central Coast to Southern (Bac Lieu, Ben             date. The salts were categorized into two
Tre, Vung Tau and Can Gio). Thus, table              types: coarse (P), refined (R) including
salt is one of the main transport pathways           non-iodized and iodized (Table S1).
of substances including MPs from aquatic             In addition, refined salts are manually
environment to human. In addition, airborne,         packaged in 1 - kg plastic bag without
refinery, packaging and transportation are           any descriptions and sold in local
highly feasible contamination sources since          markets (Figure 1). They are regarded as
sea-salt manufacturing processes are open.           non-branded salts.

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Investigation of Microplastic Contamination in Vietnamese Sea Salts Based on Raman and Fourier-Transform Infrared Spectroscopies
V. T. K. Khuyen et al / EnvironmentAsia 14(2) (2021) 1-13

        Figure 1. Vietnamese commercial sea salts branded (a) and non-branded (b)

2.2 Sample preparation procedure                      polystyrene (PS) and acrylonitrile butadiene
                                                      styrene (ABS) pellets from Vietnam plastic
     A weight of 200 g of salts was transferred       production companies.
into a glass bottle. The sample in one bottle             The MPs analysis is subject to
was a replicate, and three replicates were            determine individual particles by using
processed to receive representative results of        a Raman microscope (XploRA Horiba
each brand. A particular volume of filtered           Scientific). The microscopic mode was used
water was added and stirred until obtaining           to observe shape and color and their sizes
the homogenized brine solution. Then 30%              were manually calculated. The number of
hydrogen peroxide was added and stirred at            pieces was noted down and classified into
70 - 80°C until the solution was discolored,          shape, color categories (Hidalgo-Ruz et al.,
and stored in dark at room temperature                2012). The Raman spectrometer included
(30 ± 5°C, 60 ± 10% moisture) overnight.              a single beam laser operating at 532 nm of
     The extraction was carried out on the            wavelength coupled with a charge-coupled
funnel so that micro-particles could be               device (CCD) detector. Every individual
captured in the center of filter papers. The          particle was analyzed in a wavelength range
matter in the solution was captured on                of 50 to 3600 cm-1 with an acquisition time
1.6-μm pore size, 47-mm diameter glass fiber          of 15 seconds and a grating of 900 lines
(GF) membrane (GE Healthcare Whatman).                per mm.
It depends on the capacity of filter paper, for           For larger pieces with complex Raman
each sample, the filtration was divided into          spectra, the FT-IR spectroscopy (PerkinElmer
stages: (i) light and floated MPs; (ii) heavy         Frontier, USA) was used to reaffirm the
MPs mixed with undissolved impurities. All            polymer type. The visible item was transferred
filter membranes were kept in Petri dishes,           onto KBr membrane and its IR spectrum
carefully wrapped and let for drying at room          was recorded in the range of mid-infrared
temperature for further analysis.                     wavelength (450 – 4000 cm -1 ) in both
                                                      transmission and absorbance modes.
2.3 Microplastic analysis
                                                      2.4 Quality control
    The library included GF membrane,
polyethylene terephthalate (PET) 300 µm,                  Two types of blank samples were tested
low–density polyethylene (LDPE) 300 µm,               simultaneously to correct the potential
un-plasticized polyvinyl chloride (uPVC)              procedural contamination. The air controls
250 µm, poly(methyl methacrylate) (PMMA)              were clean filter membranes on glass Petri
85 µm, and nylon-6 (PA-6) 15 – 20 µm                  dishes around the working area. The water
purchased from Goodfellow (UK); and,                  controls were processed by the same sample
high – density PE (HDPE), polypropylene (PP),         procedure with filtered water.

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2.5. Statistical analysis                           so time - consuming and, in some cases,
                                                    relatively complicated. In fact, the steps were
     One-way analysis of variance (ANOVA)           based on properties of each particular sample.
was chosen to establish differences in MPs          Depending on the cleanness of salt samples,
abundance (items/kg) between products.              hydrogen peroxide is necessary to oxidase
A Turkey’s multiple comparison test was used        dissolved organic compounds and discolored
analyze the mean differences. All statistical       the solution mixture to a large extent, which
analyses were performed at 95% confidence           is beneficial for the following MPs floatation
interval on SPSS v22 software.                      and filtration. The solution discoloration
                                                    and matter distribution was clearly observed
3. Results and Discussion                           after overnight static incubation (Figure 2c of
                                                    G - branded salt), compared with the status
3.1 Evaluation of sample handling process           once peroxide was added (Figure 2b). The
                                                    results show that the oxidation and amount of
    There are different sample treatment            oxidants varied by samples, particularly large
procedures to extract MPs from sea salts.           amount of hydrogen peroxide was required for
The whole handling process is generally             non-branded and some of branded products.

  Figure 2. The oxidation steps in the samples preparation: (a) before oxidation, (b) once
                        adding H2O2, (c) after oxidation – brand G

              Figure 3. Microplastics found in the supernatant of brand G (a, b),
 and in the sediment of brand N (c, d). Pink circles are visible stone grains, black circles are
  visible undissolved salt particles, blue circle is invisible fiber which was observed at the
                        magnification x10 under Raman microscope

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     The insoluble impurities are the main                 and in some cases, the real colors were slightly
interference. The remaining products such as               different from visible colors (Figure 4d).
algae fibers floating on the surface could be              A very hot needle pointed directly at particle
removed easily after oxidation, while others tend          bundles and plastic debris will produce melting
to experience temporal accumulation, commonly              odor or get curl especially fibers. If bundles were
sedimentation in the water column (Wang et al.,            broken, they were not plastics. If they changed
2016). The MPs distribution in the mixture has             the cellular structure, twisted and stuck around
changed over time and their movements are easily           the needle, they would be microalgae filaments.
affected by physical forces during the incubation                Microplastics were classified into two
with hydrogen peroxide and filtration process. In          shape groups: (i) fibers and (ii) non-fibers. In the
addition, smaller MPs preferentially aggregate             former group, pieces are fibrous or thin uniform
with other matter due to strong Brownian motion            plastic strands, but not segmented (Zhang
to form larger and heavier precipitated particles          et al., 2019). The fibers should be equally thick
(Sun et al., 2020). Thus, long time oxidation is           throughout their entire length so that Raman
not recommended to minimize the aggregation                spectra could be exactly produced (Figure 4e).
of MPs with impurities. To avoid missing                   If the fibers were too thin and segmented, they
MPs, the extraction were carried out by stages             would be organic fibers (Figure 4f). In later
depending on the capacity of filter membranes.             group, MPs were observed in pellets (rounds
The first stage called supernatant, and the last           with solid structure, cylindrical, flat, sphere,
stage so-called sediment should be paid attention          ovoid), fragments (round, sub - round), or
to. Filter membranes of supernatant filtrate had           other shapes (rectangle, triangle, angular,
brighter (Figure 3a) with clearer micro plastic            sub-angular). The last care is overlapping
observation at x10 magnification (Figure b)                between particles. Invisible MPs, especially
than the sediment’s membranes (Figure 3c, d).              microfibers were encountered on the surface of
As many particles were on the membranes,                   larger MPs (Figure 4g).
physical methods were firstly applied for fast                   The polymer identification is based on
characterization. The suspected particles were             specific bands caused by vibrations of the
analyzed with Raman spectroscopy.                          functional groups (Table 2). An example is
                                                           taken in Figure 5. In the initial observation,
3.2 Microplastic characterizations                         it is a pink non-fiber fragment. As the piece
                                                           was seen by the naked eyes (1 mm in length),
     MPs could be recognized by the physical               it could be analyzed by both FT-IR and Raman
appearance. Many impurities with certain                   microscope. In Raman spectrum (Figure 5a),
shapes similar to MPs might be distinguished               a medium band at 1440.82 cm-1 is subjected to
by the color. The impurities usually appeared              -CH2- bending. The last signal is a strong band
in dim black color and unclear shapes (Figure              at 2852.09 cm-1, which assigns to stretching
4a) while the color of MPs should be clear and             vibrations of C - H bonds. The second
homogenous (Figure 4b) (Hidalgo-Ruz et al.,                intensive band at 830.103 cm-1 is in the border
2012). Since plastic products were produced                of 550 - 800 cm-1 range of vibrations of C - Cl
with various colors, colored particles such as             bonds. However, a different study assigned
yellow or pink would be highly potential MPs.              stretching vibrations of C - Cl bonds to
The color was categorized in 4 groups – (i)                bands at 635 and 695 cm-1 (Solodovnichenko
 light color, (ii) mid color, (iii) dark color             et al., 2016). The comparison with the library
(Hidalgo - Ruz et al., 2012). If the filter                shows its spectrum looks like the spectrum
membrane was seen through homogenous                       of PVC and PS. Hence, it is necessary to use
pieces, the pieces were called colorless (group iv).       an additional method to confirm the polymer
Brown debris was investigated carefully to avoid           type. Three regions should be taken into
the false identification with sand or mud particles        account in FT - IR spectrum (Figure 5b).
which tended to gather and overlap plastic                 Firstly, 1200-1400 cm-1 is assigned to
particles (Figure 4c). A melting test was applied          C - H bonds, particularly medium peak
to separate sand, salt particles from visible              at 1426.57 cm -1 in bending structure of
whitish and brown MPs (De Witte et al., 2014)              CH2-, 1254.87 cm-1 (Ul-Hamid et al., 2015)

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Investigation of Microplastic Contamination in Vietnamese Sea Salts Based on Raman and Fourier-Transform Infrared Spectroscopies
V. T. K. Khuyen et al / EnvironmentAsia 14(2) (2021) 1-13

and 1322.96 cm -1 of C-H bending (Jung               to C - C stretching. The most important
et al., 2017). This medium band of CH2-              band is 614.30 cm-1 corresponding to C - Cl
was also observed in Raman spectra at                stretching (Ul - Hamid et al., 2015; Jung
1440.82 cm-1. The CH2- rocking is present            et al., 2017). This fragment is now believed to
at 961.65 cm -1 (Ul-Hamid et al., 2015).             be PVC as only PVC has C - Cl bonds amongst
A relative small band at 1094 cm-1 is assigned       the plastic types within this study scope.

     Figure 4. The recognition of micro plastics by naked eyes and stereomicroscope:
   (a) Blurred items were discarded, and b) Shaped items were highly potential MPs; (c)
  Distiguish mud and sand particles with microplastics under microscope; (d) Distinguish
  white salt particles and non-plastic items with visible MPs by smelting test and observe
  color of MPs under the stereomicroscope; (e) Anthropogenic microfibers and f) Organic
                  bundle of fibers; (g) The overlap between micro plastics.

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Table 1. IR and Raman band assignments of seven investigated polymers

(a) Ung et al (2017); (b) Daniel and Hélio (2019); (c) Gopanna et al (2018);
(d) Solodovnichenko et al., 2016; (e) Anwar Ul-Hamid et al. (2015);
(f) Gündoğdu (2018); (g) Käppler et al. (2015); (h) Alexiou et al. (2020);
(i) Bruckmoser et al. (2015); (j) Milani (2015)

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  Figure 5. Polymer identification of a visible potential microplastic: Raman spectrum (a)
                                  and FT-IR spectrum (b).

3.3. The microplastics abundance in                    For this reason, the quality of non - branded
commercial sea salts                                   salts was affected by not only the seawater
                                                       where their pellets were crystalized but
     As seen in Figure 6, the proportions              also the quality of local water sources,
of MPs were generally below 50% of the                 production environment where the salt
total number of potential plastics identified          coarse were ground and minced salts were
by microscopic observation in individual               packaged. As a result, the MPs quantities
sediment and supernatant parts. More                   varied in the markets. Can Gio non-
importantly, the majority of MPs were                  branded salts contained a low level of MPs,
partitioned in the supernatant. For instance,          only 56.98 ± 23.81 items/kg. The MPs
the percentage of MPs was only 3 - 5%                  abundance in salts bought at the urban
sediment in G - brand salts, and no micro              markets of Ho-Chi-Minh city varies in
plastics were found in the sediment of other           a small range (48.93 ± 25.14 to 119.07
brands (E, F, J, K L, M). However, MPs                 ± 71.80 items/kg). Nevertheless, there
accounted for high proportions in the sediment         was a considerably high number of MPs
of several brands (A, B, I and N). Although            in salts purchased at a suburban district,
the number of particles depositing in the              up to 402.52 ± 168.43 items/kg (MM4).
sediment of non-branded products was higher            Indeed, the Turkey’s test shows that
than those of branded salts, the most of them          only MM4 was statistically different
were grain stones. These stone particles had           from other non-branded samples (Sig.
similar appearances to plastic particles, for          values < 0.014, Table S2c). Figure 7a
example, homogenous black, pink and yellow             shows that PET accounted for highest
particles (Figure 3). Brand B is a special case.       percentage (33.63%), followed by
The vast sum of MPs was encountered in the             PE (24.78%), PS (20.35%) and PVC
sediment of salt coarse particle (B.P), while          (8.85%). In contrast, PP and acrylic
they tended to distribute in the supernatant of        glass were not dominant. Almost non-
refined type (B.R sample).                             fibrous items were fragments smaller
     The ANOVA analysis shows statistically            than 50 µm, and their minimal size
significant differences in MPs amount                  was 20 µm. Nevertheless, for fibrous
between non-branded salts (n = 6, Sig.                 structures, long fibers ( >100 µm) were
= 0.00185, Table S2b), possibly because                predominant, and only there were few
contaminations came from different                     of fibers longer than 500 µm.
sources. The refined salt is more widely used               The plastic composition in brands was
in daily cooking and meals. Therefore, non-            different from non-labeled salts. The period
branded fine salts were manually produced              of use of branded salts is one to two years
on the household scales or local factories.            from the production date. The samples

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were analyzed within 5 months from the             differences between brands (n = 14,
production date. Hence, the possibility            Sig. = 0.473 - ANOVA in Table S3b. In
of transferring PP micro plastics from             addition, the Turkey’s test shows that
polypropylene packages to the salts inside         there were no differences among branded
was fairly low. PET was again dominant             products with the minimum Sig. value of
(51.74%), followed by PE (23.7%) (Figure           0.483 (Table S3c – Supplementary data).
7b). The findings are similar to Spanish           The salts had similar quality because the
(Iñiguez et al., 2017) and Chinese salts           process and quality were controlled to
(Yang et al., 2015). It is suggested heavy         meet Vietnam’s food safety regulations.
PET debris tended to move to the bottom            Nevertheless, in this group, the presence
and remain with the salt during the salt           of MPs varies from pellet to refined salts,
crystallization because of high density (1.3       which reveals the effects of salt processing
g/cm3) compared with PE (0.94 g/cm3) and           on MPs accumulation in salts to some
PP (0.90 g/cm3) (Yang et al., 2015).               extent. In refined salts, the amount of
    On the other hands, MPs amounts did            insoluble impurities was lower, thereby
not demonstrate statistically significant          requiring simpler sample treatment.

                Figure 6. The partition of micro debris in the saline mixture

         Figure 7. Anthropogenic pieces in non-branded (a) and branded salts (b).

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MPs might be removed along with the                 3.4. The enhanced toxicity of MPs-contaminated
impurities. This is why refined type                salt consumption
contained 140 plastic items slightly lower
than the pellet type (178 items/kg) in brand             The daily salt intake has increased by
A. In contrast, salts could be contaminated         50 times in the civilized societies, average
with MPs from the environment during                from 9 to 12 g/day today. WHO recommends
the grinding and refinery, leading to the           a healthy level of 5.1 g salt because an
higher number of MPs in the refined                 inappropriate salt/sodium intake has resulted
salts (201 items) compared with 144                 in kidney diseases, hypertension, and mortality
items/kg in salt pelelts of brand B. PVC            (Partearroyo et al., 2019). The daily salt intake
fibers was most predominant in fine salts           varies from genders, ages to countries, and is
though PVC is not a common plastic in               not fully conscious during cooking or at the
the sea water. Hence, PVC from water                table (Partearroyo et al., 2019). Therefore, the
pine could probably intrude into the salt           exact amount of daily digested plastic items
during the refinery. In Figure 8, PMMA              via salts cannot well established.
did not appear in salt pellets, whilst small             The annual MPs intake was calculated
particles were present in refined salts. PE         on daily salt consumption of 10 g/day (Table
fibers dominated while PA-6 fibers were             3). Though MPs in Turkish sea salts were
not found in the coarse salts. In contrast          relatively low, the salt consumption was
long fibers were found in fine salts that           very high, up to 18.5 g per day, leading to
could come from the clothing of workers             a high number of 554.95 MPs consumed per
at the factory. PE, PVC and PMMA pieces             year. In almost European countries, the daily
were commonly in transparent and light to           salt consumption is 7 to 13 g/day in adults
mid colors (white, blue), while PET and             (Partearroyo et al., 2019), and an estimated
PS pieces were in mid to dark colors (blue,         of over 1000 plastic pieces could be digested
orange, purple). There was no presence              via Spanish salts (Iñiguez et al., 2017).
of green and white-striped black items              The MPs contents in Vietnamese, Indonesian
in all salts.                                       and Korean commercial salts were similar
     No MPs or impurities were seen in              (Kim et al., 2018), much higher than
water control. Several black particles              Taiwanese (9.77 particles/kg) (Lee et al.,
were on air controls, however, they were            2019). The amount of MPs in Chinese
dust. MPs frequently appeared when the              sea salts was highest, up to 718 particles/
laboratory was so crowded, but in a small           kg (Yang et al., 2015) relevant to 2620
amount, only 5 ± 2 particles/filter. It is          MPs ingested annually as effects of heavy
strongly believed contamination from the            plastic pollution in 81% of coastal regions
laboratory coat did not occur because of no         according to the Chinese State Oceanic
cotton fibers found                                 Administration (SOA) (Wang et al., 2019).

Figure 8. Composition and size of microplastics in branded coarse salts (a) and refined salts (b)

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Table 3. Summarized information of in commercial sea salts and their annual
intake via salt consumption route in the world

      Humans are ingesting hundreds of MPs              combination harmful effects of inappropriate
from salt alone. Nonetheless, the particular            salt consumption and hundreds of plastic
harmful effects of MPs on human health are              particles via salt digestion every year.
still being investigated. MPs of 0.2 to 150 µm               The success of salt reduction depends
were believed to translocate across living cells        on eating habits of consumers and objective
to intestinal lymphatic and circulatory systems         factors such as availability, food accessibility
of human body (Hussain et al., 2001), but their         and salt costs on the market. On Vietnamese
mechanisms has not fully understood yet. A              salt market, in no-name bags varied
study reported the human’s excretory system             noticeably, up to 402.67 items/kg, compared
could itself eliminate over 90% of micro                with maximum of 276.25 items/kg in
to nano - plastic debris via feces (Peixoto             branded salts. Hence, it is so necessary to
et al., 2019) while the 10% were absorbed               add MPs parameter in food safety standards,
into the blood steam and actually their                 control more strictly the quality and price of
behaviours in our body are well unknown.                non - labeled salts as well.
Bioaccumulation of non-degradable plastics
is dangerous because they can be a home                 Supplementary data
for microorganisms, hydrophobic organic
substances and heavy metals.                               Supplementary data are available at
                                                        EnvironmentAsia Journal website.
4. Conclusions
                                                        Acknowledgment
     Although there were differences in the
number of MPs in brands and no - name                       This research was funded by German
salts, the average of MPs was finally similar           Academic Exchange Service (DAAD) under
(133.62 items/kg), which shows MPs                      the doctoral program Sustainable Water
contamination in seawater of coastal salt               Management 2018. The authors gratefully
production locations was similar in Vietnam.            thank the support of MSc. Nguyen Vu Duy
PET was dominant followed by PE and                     Khang at Vietnam Academy of Science
PP, whilst PMMA was the least common.                   and Technology for facilities and scientific
Vietnamese people are suffering from                    equipment.
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References                                           Hidalgo-Ruz V, Gutow L, Thompson RC,
                                                        Thiel M. Microplastics in the Marine
Alexiou VF, Mathioudakis GN, Andrikopoulos              Environment: A Review of the Methods
   KS, Soto Beobide A, Voyiatzis GA.                    Used for Identification and Quantification.
   Poly(ethylene Terephthalate) Carbon-                 Environmental Science and Technology
   Based Nanocomposites: A Crystallization              2012; 46(6): 3060-3075.
   and Molecular Orientation Study.                  Hussain N, Jaitley V, Florence AT. Recent
   Polymers (Basel) 2020; 12(11): 1-14.                 advances in the understanding of
Ul-Hamid A, Soufi KY, Al-Hadhrami LM,                   uptake of microparticulates across the
   Shemsi AM. Failure investigation of an               gastro-intestinal lymphatics. Advanced
   underground low voltage XLPE insulated               Drug Delivery Reviews 2001; 50: 107-142.
   cable Anti-Corrosion. Methods and                 Iñiguez ME, Conesa JA, Fullana A.
   Materials 2015; 62(5): 281-287.                      Microplastics in Spanish Table Salt.
Bruckmoser K, Resch K, Kisslinger T, Lucyshyn           Scientific Report 2017; 7(8630): 1-7.
   T. Measurement of interdiffusion in               Jensen PN, Tran QB, Tran TTH, Heckbert
   polymeric materials by applying Raman                SR, Annette LF, James PL, Truong LVN,
   spectroscopy. Polymer Testing 2015; 46:              Mokdad AH. The association of estimated
   122-133.                                             salt intake with blood pressure in a Viet Nam
José da Silva D, Wiebeck H. Predicting                  national survey. PloS ONE 2018; 13(1): 1-12.
   LDPE/HDPE blend composition by                    Jung MR, Horgen FD, Orski SV, Rodriguez
   CARS-PLS regression and confocal                     VC, Viviana, Beers KL, Balazs GH,
   Raman spectroscopy. Polímeros 2019;                  Jones TT, Work TM, Brignac KC, Royer
   29(1): 1-11.                                         SJ, Hyrenbach KD, Jensen BA, Lynch
Witte BD, Devriese L, Bekaert K, Hoffman                JM. Validation of ATR FT-IR to identify
   S, Vandermeersch G, Cooreman K,                      polymers of plastic marine debris, including
   Robbens K. Quality assessment of                     those ingested by marine organisms. Marine
   the blue mussel (Mytilus edulis):                    Pollution Bulletin 2018; 127: 704-716.
   Comparison between commercial and                 Karami A, Golieskardi A, Choo CK, Larat V,
   wild types. Marine Pollution Bulletin                Galloway TS, Salamatinia B. The presence
   2014; 85(1): 146-155.                                of microplastics in commercial salts from
Erni-Cassola G, Gibson MI, Thompson                     different countries. Scientific Report
   RC, Christie-Oleza JA (2017). Lost,                  2017a; 7(46173): 1-9.
   but Found with Nile Red: A Novel                  Käppler A, Windrich F, Löder MGJ, Malanin
   Method for Detecting and Quantifying                 M, Fischer D, Labrenz M, Eichhorn KJ,
   Small Microplastics (1 mm to 20 μm) in               Voit B. Identification of microplastics by
   Environmental Samples. Environmental                 FTIR and Raman microscopy: a novel
   Science and Technology 2017; 51(23):                 silicon filter substrate opens the important
   13641-13648.                                         spectral range below 1300 cm-1 for FTIR
Gopanna A, Mandapati RN., Thomas SP,                    transmission measurements. Analytical
   Rajan K, Chavali M. Fourier transform                and Bioanalytical Chemistry 2015; 407:
   infrared spectroscopy (FTIR), Raman                  6791-6801.
   spectroscopy and wide angle X ray                 Kim JS, Lee HJ, Kim S K, Kim HJ. Global
   scattering (WAXS) of polypropylene                   pattern of microplastics (MPs) in
   (PP)/cyclic olefin copolymer (COC)                   commercial food- grade salts: sea salt as
   blends for qualitative and quantitative              an indicator of seawater MP pollution.
   analysis. Polymer Bulletin 2019;                     Environmental Science and Technology
   76:4259-4274.                                        2018; 52: 12819-12828.
Gündoğdu S. Contamination of table salts             Kosuth M, Mason SA, Wattenberg EV.
   from Turkey with microplastics, Food                 Anthropogenic contamination of tap
   Additives & Contaminants Part A 2018;                water, beer, and sea salt. PLoS ONE 2018;
   35(5): 1006-1014.                                    13(4): 1-18.

                                                12
V. T. K. Khuyen et al / EnvironmentAsia 14(2) (2021) 1-13

Lebreton L, Andrady A. Future scenarios of              Solodovnichenko VS, Polyboyarov VA,
   global plastic waste generation and disposal.           Zhdanok AA, Arbuzov AB, Zapevalova
   Palgrave Communications 2019; 5(6): 1-11.               ES, Kryazhev Yu.G, Likholobov VA.
Le DT. Overview of Marine Plastic Debris                   Synthesis of carbon materials by the
   in Vietnam in Relation to International                 short-term mechanochemical activation of
   Context FIG Working Week 2019.                          polyvinyl chloride. Procedia Engineering
   Geospatial information for a smarter                    2016; 152: 747-752.
   life and environmental resilience Hanoi,             Sun H, Jiao R, Wang D. The difference
   Vietnam, April 22-26, 2019.                             of aggregation mechanism between
Lee H, Kunz A, Shim JW, Walther BA.                        microplastics and nanoplastics: Role of
   Microplastic contamination of table salts               Brownian motion and structural layer
   from Taiwan, including a global review.                 force. Environmental Pollution 2020; 268
   Scientific Report 2019; 9(10145): 1-9.                  (Part B): 115942.
Milani A. Unpolarized and Polarized Raman               Urbanek AK, Rymowicz W, Mironczuk
   Spectroscopy of Nylon-6 Polymorphs:                     AM. Degradation of plastics and plastic-
   A Quantum Chemical Approach. Journal                    degrading bacteria in cold marine habitats.
   of Physical Chemistry B 2015; 119(9):                   Applied Microbiology and Biotechnology
   3868-3874.                                              2018; 102: 7669-7678.
Partearroyo T, Samaniego-Vaesken ML,                    Wang MH, He Y, Sen B. Research and
   Ruiz E, Aranceta-Bartrina J, Gil A,                     management of plastic pollution in coastal
   González-Gross M, Ortega RM,                            environments of China. Environmental
   Serra-Majem L, Varela-Moreiras G.                       Pollution 2019; 248: 898-905.
   Sodium Intake from Foods Exceeds                     Wang J, Tan Z, Peng J, Qiu Q, Li M. The
   Recommended Limits in the Spanish                       behaviors of microplastics in the marine
   Population: The ANIBES Study. Nutrients                 environment. Marine Environmental
   2019; 11(10): 24-51.                                    Research 2016; 113: 7-17.
Peixoto D, Pinheiro C, Amorim J,                        Yang D, Shi H, Li L, Li J, Jabeen K,
   Oliva-Teles L, Guilhermino L, Vieira MN.                Kolandhasamy P. Microplastic Pollution
   Microplastic pollution in commercial                    in Table Salts from China. Environmental
   salt for human consumption: A review.                   Science and Technology 2015; 49(22):
   Estuarine, Coastal and Shelf Science 2019;              13622-13627.
   219: 161-168.                                        Zhang C, Zhou H, Cui Y, Wang C, Li Y, Zhang
Rezania S, Park J, Din MFM, Shazwin MT,                    D. Microplastics in offshore sediment
   Amirreza T, Yadav KK, Kamyab H.                         in the Yellow Sea and East China Sea,
   Microplastics pollution in different aquatic            China. Environmental Pollution 2019;
   environments and biota: A review of recent              244: 827-833.
   studies. Marine Pollution Bulletin 2018;             Zhang Q, Xu GE, Li J, Chen Q, Ma L, Zeng
   133: 191-208.                                           EY, Shi H. A Review of Microplastics in
Paulo RC, Nolasco MM, Araújo C. Chapter                    Table Salt, Drinking Water, and Air: Direct
   5 – Characterization of Microplastic by                 Human Exposure. Environmental Science
   Raman Spectroscopy. In: Comprehensive                   & Technology 2020; 54(7): 3740-3751.
   Analytical Chemistry, Vol 75, 2017,
   p.119-151.
Seth CK, Shriwastav A. Contamination
   of Indian sea salts with microplastics
   and a potential prevention strategy.
   Environmental Science and Pollution
   Research 2018; 25: 30122-30131.

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