Annual Congress 2019 The Danish Microbiological Society - 11 NOVEMBER 2019 COPENHAGEN DENMARK - dms.dk

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Annual Congress 2019 The Danish Microbiological Society - 11 NOVEMBER 2019 COPENHAGEN DENMARK - dms.dk
Programme & Abstracts

The Danish Microbiological Society

Annual Congress 2019

11 NOVEMBER 2019
     EIGTVEDS PAKHUS
  COPENHAGEN · DENMARK

                            www.dmselskab.dk
Annual Congress 2019 The Danish Microbiological Society - 11 NOVEMBER 2019 COPENHAGEN DENMARK - dms.dk
Index
Programme  .            .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .     4

Flash poster presentations .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  . 7

About DMS  .           .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .   9

Speakers’ abstracts .                     .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .     11

Industry symposia  .                      .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .  .   31

Poster sessions and Poster Index  .                                             .  .  .  .  .  .  .  .  .  .  .  .  .  .    35
Annual Congress 2019 The Danish Microbiological Society - 11 NOVEMBER 2019 COPENHAGEN DENMARK - dms.dk
Programme
09.00   Registration, poster mounting and coffee                                                         12:35-   Bruker Symposium:
                                                                                                         13:00    ISO 16140-6 validation of Maldi Biotyper (see p. 31) ROOM II, second floor
                   ROOM II Second floor                              ROOM III Second floor
                                                                                                         13:10-   Nordic Biosite Symposium:
10.00   Welcome and opening address by                   Welcome and opening address by                  13:35    Standardizing microbiomics - removing bias in collection,
        DMS President                                    DMS Vice-president                                       purification and analyses (see p. 31) ROOM II, second floor
        Carsten Suhr Jacobsen                            Trine Rolighed Thomsen
                                                                                                                                                      PARALLEL SESSIONS
10.15   [O01] Citizen science in systematic and          [O02] Vaccine hesitancy – a major threat
        applied microbiology                             for global health                                        ROOM II Second floor             ROOM III Second floor            ROOM IV Second floor
        Speaker: Preben Nielsen, Novozymes               Speaker: Kåre Mølbak, Statens Serum Institut
                                                                                                                  Big data and deep learning       Novel antimicrobial              The challenge of slow
10.45   Coffee and exhibition First & second floor                                                                meets microbiology               therapies                        growing bacteria
                                                                                                                  Chair: Mads Albertsen,           Chair: Ole Højberg, Aarhus       Chair: Peter Stougaard,
                                             PARALLEL SESSIONS                                                    Aalborg University               University                       Aarhus University
        ROOM II Second floor              ROOM III Second floor          ROOM IV Second floor                     DMS co-chair: Trine              DMS co-chair: Rikke Louise       DMS co-chair: Kasper
                                                                                                                  Thomsen, Teknologisk             Meyer, Aarhus University         Nørskov Kragh, University of
        Biodiversity and                  Vaccines                       Microbial food web in soil               Institut                                                          Copenhagen
        biogeography                      Chair: Anders Miki Bojesen,    Chair: Tim Urich, Universität
        Chair: Per Halkjær Nielsen,       University of Copenhagen       Greifswald                      13.45    Chair introduction               Chair introduction               Chair introduction
        Aalborg University                DMS co-chair: Mette            DMS co-chair: Carsten Suhr      13.50    [O12] Microbes inside and        [O15] ICARS - The                [O18] Microbiological and
        DMS co-chair: Trine               Burmølle, University of        Jacobsen, Aarhus University              out                              International Center for         pathogenesis features
        Thomsen, Teknologisk              Copenhagen                                                              Daniel Mc Donald, University     Antimicrobial Resistance         distinguishing Lyme
        Institut                                                                                                  of California San Diego, USA     Solutions                        disease and relapsing fever
11.00   Chair introduction                Chair introduction             Chair introduction                                                        Robert Skov, Statens Serum       spirochetes
                                                                                                                                                   Institut                         Sven Bergström, Umeå
11.05   [O03] Microflora Danica           [O06] Preventing disease       [O09] Predatory bacteria                                                                                   University
        meets Earth Microbiome            and antimicrobial use in       in soil: key-stone taxa of
        Project                           animals by vaccination         microbial foodwebs?             14.15    [O13] Binning microbial          [O16] Fecal transplants          [O19] Tuberculosis
        Per Halkjær Nielsen,              Anders Miki Bojesen,           Tim Urich, Universität                   genomes using deep               – the ultimative probiotic       – diagnosis from a
        Aalborg University & Daniel       University of Copenhagen       Greifswald, Germany                      learning                         for pigs?                        laboratory point of view
        McDonald, University of                                                                                   Simon Rasmussen,                 Nuria Canibe, Aarhus             Dorte Bek Folkvardsen,
        California, San Diego, USA                                                                                University of Copenhagen         University                       Statens Serum Institut

11.30   [O04] Project SoilTracker         [O07] Using viruses to fight   [O10] Significance of micro-    14.30    [O14] Trash to treasure:         [O17] Local drug delivery to     [O20] Slow-growth and
        - solving crimes with soil-       viruses – perspectives for     predator diversity in the                exploring the wastewater         target biofilm infections        no-growth in natural
        microbiodiversity                 future vaccines                rhizosphere                              system with high quality         Rikke Meyer, Aarhus              environment
        Tobias Guldberg Frøslev,          Allan Randrup Thomsen,         Flemming Ekelund,                        MAGs from large-scale            University                       Hans Røy, Aarhus University
        Centre for GeoGenetics,           University of Copenhagen       University of Copenhagen                 Nanopore data
        University of Copenhagen                                                                                  Caitlin Singleton, Aalborg
                                                                                                                  University
11.45   [O05] Atmospheric                 [O08] Adjuvants: From          [O11] Patterns of
        microorganisms in the             immunologist’s dirty little    microbiome predator             14.45    Flash poster presentations       Flash poster presentations       Flash poster presentations
        Arctic                            secret to vaccinologist’s      communities at a global                  (see page 7)                     (see page 7)                     (see page 7)
        Tina Santl-Temkiv, Aarhus         requisite for success          scale                           15.00    Coffee and exhibition First & second floor
        University                        Dennis Christensen, Statens    Stefan Geisen,
                                          Serum Institut                 Department of Terrestrial       15.15    POSTER SESSION (UNEVEN NUMBERS)
                                                                         Ecology, Netherlands                     SALON B, ground floor & first floor
                                                                         Institute of Ecology, NIOO-
                                                                         KNAW                            15:15-   Leymus Genomics Symposum:
                                                                                                         15:40    Long-read single molecule on Illumina instruments for a ground-breaking new level
12.00   Flash poster presentations        Flash poster presentations     Flash poster presentations               of bacteria identification (see p. 33) ROOM II, second floor
        (see page 7)                      (see page 7)                   (see page 7)
                                                                                                         15:50-   Clinical Microbiomics Symposium:
12.15   Lunch Salon C, ground floor                                                                      16:15    Nothing in biology makes sense except in the light of evolution (see p. 33) ROOM II, Second floor
12.15   Exhibition First & second floor                                                                  16.15    Pasteur travel grant ceremony ROOM III, Second floor
12.35   GENERAL ASSEMBLY Det Danske Pasteur Selskab ROOM IV, second floor                                16.30    [O21] Keynote by Kevin Foster ROOM III, Second floor
                                                                                                                  Cooperation and competition in bacteria: from model systems to the microbiome
12.45   POSTER SESSION (EVEN NUMBERS) SALON B, ground floor & first floor
                                                                                                         17.30    Reception with fermented beverage SALON C, ground floor
                                                                                                         19.00    Optional congress dinner Spiseloppen, Christiania

4                                                                                                                                                                                                                 5
Annual Congress 2019 The Danish Microbiological Society - 11 NOVEMBER 2019 COPENHAGEN DENMARK - dms.dk
novozymes.com

                                                                          Flash poster presentations
                                                                                                             PARALLEL SESSIONS, MORNING
                                                                                     ROOM II Second floor            ROOM III Second floor          ROOM IV Second floor
                                                                                  Biodiversity & biogeography     Vaccines                       Microbial food web in soil
                                                                          12.00   [P021] Metagenomics             [P077] Campylobacter phages    [P049] Two novel
                                                                                  profiling uncovers diversity,   mimic the host defense         bacteriophage genera
                                                                                  ecological success and          strategy by using phase        discovered in a groundwater
                                                                                  habitat preference of           variation to create new        reservoir indicate large
                                                                                  comammox nitrospira             phenotypes with modified       predator-prey diversity in
                                                                                  Barth Smets, Technical          receptor binding proteins      subsurface environments
                                                                                  University of Denmark           Martine Sørensen,              Ole Hylling,
                                                                                                                  University of Copenhagen       Aarhus University
                                                                          12.05   [P013] Smoke on the water:      [P101] One size does not       [P050] Biological drivers
                                                                                  Drafting the genome             fit all; the gap between       of bacterial communities
                                                                                  of the pigmented algea          standardized in vitro          in the arctic water inflow
                                                                                  mesotaenium berggrenii          biofilm-infected wound         region revealed through
                                                                                  that darkens the greenland      models and in vivo clinical    manipulations of microbial
                                                                                  ice sheet                       settings                       food web interactions
                                                                                  Athanasios Zervas,              Ida Clement Thaarup,           Oliver Müller,
                                                                                  Aarhus University               University of Copenhagen       University of Bergen
                                                                          12.10   [P075] MIDAS 4: A               [P097] Effect of A-hemolysin   [P071] Multi-kingdom
                                                                                  comprehensive reference         producing e. coli in two       microbial communities in

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                                                                                  Morten Simonsen Dueholm,        Hengameh Chloe
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                                                                                                                  Statens Serum Institut

in biological                                                                                               PARALLEL SESSIONS, AFTERNOON

solutions
                                                                                  Big data and deep learning      Novel antimicrobial            The challenge of slow
                                                                                  meets microbiology              therapies                      growing bacteria
                                                                          14.45   [P036] Increasing the           [P004] Antibacterial           [P089] Domain analysis
Together with customers, partners and the global                                  phylogenetic resolution         cue from ecological            of the cell wall proteinase,
                                                                                  of pseudomonas in soil          neighbor increases             lactocepin, of lactococcus
community, we improve industrial performance while                                microbiomes by rpod-            holomycin production in        lactis
preserving the planet’s resources and helping to build                            specific primers                photobacterium galatheae       Ida Nynne Laforce,
                                                                                  Jonas Greve Lauritsen,          Yannick Buijs, Technical       National Food Institute
better lives. As the world’s largest provider of enzyme                           DTU Bioengineering              University of Denmark
and microbial technologies, our bioinnovation enables                     14.50   [P037] Big production           [P008] Combination of          [P084] Deep purple: The
higher agricultural yields, low-temperature washing,                              without the use of              cannabidiol and bacitracin     biological darkening of the
energy-efficient production, renewable fuel and many                              antibiotics - impact on         against resistant bacteria     greenland ice sheet
                                                                                  the pig resistome and           Claes S. Wassmann,             Laura Halbach,
other benefits that we rely on today and in the future.                           microbiome                      University of Southern         Aarhus University
We call it Rethink Tomorrow                                                       Katrine Wegener Tams,           Denmark
                                                                                  DTU Bioengenerring
                                                                          14.55   [P043] Uncovering the           [P002] Potential of a          [P083] The oxygen
                                                                                  hidden diversity of asgard      bacteriophage cocktail         consumption rate of cable
                                                                                  archaea                         to treat rainbow trout         bacteria
                                                                                  Jakob Brandt,                   fry syndrome (RTFS):           Stefano Scilipoti,
                                                                                  Aalborg University              Comparison of delivery         Aarhus University
                                                                                                                  methods
                                                                                                                  Valentina Laura Donati,
                                                                                                                  Technical University of
                                                                                                                  Denmark

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Annual Congress 2019 The Danish Microbiological Society - 11 NOVEMBER 2019 COPENHAGEN DENMARK - dms.dk
About DMS
                                                                            The Danish Microbiological Society (DMS)         Scientific Committee
                                                                            is a professional association in the fields of
                                                                            human and veterinary medical microbiology,       Thomas Bjarnsholt
                                                                            general microbiology, food microbiology,         (Congress Chairman and Treasurer),

BIOCHEMICAL JOURNAL
                                                                            environmental microbiology and biotech-          Department of Immunology and
                                                                            nology. DMS has existed since 1958, and is       Microbiology, University of Copenhagen
                                                                            dedicated to the advancement of microbi-
                                                                            ology, both applied and basic, and promotes      Carsten Suhr Jacobsen
                                                                            microbiological information to the public.       (President of Danish Microbiological Society),
                                                                            These aims are achieved by organizing            Department of Environmental Sciences,
Molecular mechanisms in biology                                             annual congresses, workshops and symposia        Aarhus University
                                                                            - and by taking part in the current microbio-

THE NEXT HOME FOR YOUR PAPER
                                                                            logical debate.                                  Trine Rolighed Thomsen
                                                                                                                             (Vice-President of the Danish Microbiological
                                                                            Furthermore, DMS collaborates with the           Society), Danish Technological Institute and
                                                                            Danish Pasteur Society on the award of travel    Aalborg University
                                                                            grants to students and researchers in micro-
                                                                            biology, immunology and related science.         Mette Burmølle
                                                                                                                             (Secretary), Department of Biology,
                                                                            Being a member of DMS, you are part of the       University of Copenhagen
                                                                            advancement of microbiology in Denmark.
                                                                            Additionally, as a member of DMS, you are        Marie Allesen-Holm
                                                                            entitled to discounts at FEMS meetings           Novo Nordisk
                                                                            (Federation of European Microbiological
                                                                            Societies) and for FEMS journals.                Lars Bogø Jensen
                                                                                                                             National Food Institute,
                                                                                                                             Technical University of Denmark
                                                                            Contact
                                                                            The Danish Microbiological                       Kasper Nørskov Kragh
                                                                            Society Secretariat                              Department of Immunology and
                                                                            c/o CAP Partner                                  Microbiology, University of Copenhagen
                                                                            Nordre Fasanvej 113
                                  SUBMIT YOUR BEST                          DK 2000 Frederiksberg
                                                                            info@dmselskab.dk
                                                                                                                             Rikke Louise Meyer
                                                                                                                             Interdisciplinary Nanoscience Center
                                      RESEARCH AT                                                                            (iNANO) and Department of Bioscience,
                                                                                                                             Aarhus University
                                      biochemj.msubmit.net                                                                   Michael Thomas-Poulsen
                                                                                                                             Department of Biology,
                                                                                                                             University of Copenhagen

                                                                                                                             Katrine Uhrbrand
                                                                                                                             FORCE Technology

                                                                                                                             Ole Højberg
                                                                                                                             Aarhus University
                                                                                                                             Department of Animal Science

                                                                            The DMS 2019 Congress is supported by the American Society for Microbiology (ASM).
                                                                            Read more about ASM at www.asm.org

portlandpress.com/biochemj        @PPPublishing   PortlandPressPublishing
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Annual Congress 2019 The Danish Microbiological Society - 11 NOVEMBER 2019 COPENHAGEN DENMARK - dms.dk
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Annual Congress 2019 The Danish Microbiological Society - 11 NOVEMBER 2019 COPENHAGEN DENMARK - dms.dk
Speakers’ abstracts
[O01] CITIZEN SCIENCE IN SYSTE-                        [O02] VACCINE HESITANCY - A MAJOR                                                                       [O03] MICROFLORA DANICA MEETS
MATIC AND APPLIED MICROBIOLOGY                         THREAT FOR GLOBAL HEALTH                                                                                EARTH MICROBIOME PROJECT

Preben Nielsen1                                        Kåre Mølbak1                                                                                            Per Halkjær Nielsen1, Mads Albertsen2,
                                                                                                                                                               Daniel A. McDonald3
1
  Novozymes, Microbe Technology, Lyngby,               1
                                                        Statens Serum Institute, Department of Infec-
Denmark                                                tious Disease Epidemiology, Department of                                                               1
                                                                                                                                                                Aalborg University, Aalborg University, Dep. of
                                                       Infectious Disease Epidemiology, Copenhagen,                                                            Chemistry and Bioscience, Aalborg, Denmark
                                                       Denmark                                                                                                 2
                                                                                                                                                                 Aalborg University, Center for Microbial
                                                                                                                                                               Communities, Dept. Chemistry and Bioscience,
                                                                                                                                                               Aalborg, Denmark
“Masseeksperimentet 2018” was an extensive             In 2019, WHO identified vaccine hesitancy as        Research has not been able to confirm the as-       3
                                                                                                                                                                 University of California, San Diego, United States
Citizen science project conducted by school            one of the ten threats to global health. Thus,      sociation between HPV vaccine and adverse
children from all over Denmark. We wanted to           vaccine hesitancy ranks among major health          events including autonomic dysfunctions.
nurse the interest in biology and biotechnolo-         threats including ebola and other high-threat       Nonetheless, the concerns seems to linger on        How many microbial species exist on the
gy, show that “good” microbes are surrounding          pathogens, pandemic influenza, dengue and           as vaccination uptake is not yet back to the lev-   planet, and how do environments shape their
us, and that by relatively simple tools we can         antimicrobial drug resistance.                      el we saw before the crisis.                        communities? How are they distributed across
show the presence of Lactic Acid Bacteria.                                                                                                                     natural and cultivated habitats on a local and
                                                       Vaccine hesitancy is the reluctance or re-          Suboptimal vaccine coverage should not au-          global scale? These are some of the questions
In 3 weeks, 25,000 pupils collected samples            fusal to vaccinate despite the availability         tomatically be framed as vaccine hesitancy.         the two projects, “Microflora Danica” and
and tested for presence of lactic acid bacteria        of vaccines, and threatens to reverse pro-          Research shows that vaccine hesitancy is not        “Earth Microbiome Project” are trying to an-
by a simple fermentation experiment. More              gress made in tackling vaccine-preventa-            the major cause for being non-vaccinated in         swer. Novel DNA-based sequencing technolo-
than 11,000 positive samples were found and            ble diseases. Vaccination is one of the most        Denmark. While nearly all infants receive the       gies now allow a robust and high-throughput
submitted to the project. From a subset of             cost-effective ways of avoiding disease – it        first vaccine at 3 months of age, some parents      identification of microorganisms in any habi-
5,000 samples, 12,000 bacterial strains were           currently prevents 2-3 million deaths a year,       (in the busy every-day life) simply forget about    tat. Here, we present the plans and first results
identified to species level, of which 4000             and a further 1.5 million could be avoided if       vaccines when children grow older. We envis-        for Microflora Danica, or “the Microbiome of
unique isolates were cultivated, preserved in a        global coverage of vaccinations improved.           age that a new national and “smart” reminder        Denmark”, where over the next 3 years we will
strain collection and characterized by shut gun                                                            service will serve as a tool to further increase    establish a reference database over “all” mi-
WGS. Based on comparative WGS a subset of              Measles, for example, has seen a 30% increase       vaccination coverage in Denmark.                    crobes in Denmark cross various habitats. In
the strains could be affiliated to at least 10 novel   in cases globally. The reasons for this rise are                                                        conjunction with the Earth Microbiome Pro-
species. The data were presented to the pupils         complex, and not all of these cases are due to                                                          ject and The Microsetta Initiative, we applied
in a FAST-ANI tree linking up to a map of Den-         vaccine hesitancy. However, some countries                                                              the same DNA-based sequencing technique
mark showing sample locations and metadata             that were close to eliminating the disease have                                                         to a collection of over 500 samples from a di-
(see bacteriadanica.dk).                               seen a resurgence. Weak health care and con-                                                            verse set of geographically distributed envi-
                                                       sequences of conflicts may play an important                                                            ronments. These samples include host and
                                                       role - but in many settings, vaccine hesitancy is                                                       non-host associated collections, including
                                                       part of the challenge.                                                                                  multiple distinct human populations, and pro-
                                                                                                                                                               vide a glimpse of deep bacterial RNA operon
                                                       The reasons why people choose not to vacci-                                                             diversity at a global scale. We show a recapitu-
                                                       nate are complex. Lack of awareness of vac-                                                             lation short read based observations of the 16S
                                                       cine-preventable diseases, inconvenience in                                                             gene for the same samples, provide substan-
                                                       accessing vaccines, belief in advantages of                                                             tial increase in taxon specificity, and describe
                                                       natural infections, and lack of confidence to                                                           the distribution operon elements with respect
                                                       experts and authorities are key reasons un-                                                             to environmental variables. These data pro-
                                                       derlying hesitancy. In spite of research that                                                           vide a critical expansion to these open-access
                                                       confirm the safety of the MMR vaccine, myths                                                            resources pairing with other rich data gener-
                                                       about association between MMR vaccine and                                                               ation presently underway for the same Earth
                                                       autism continues to play a role in the public                                                           Microbiome Project sample set.
                                                       discourse. In Denmark, concerns about the
                                                       safety of HPV vaccines caused a temporary
                                                       set-back for the HPV vaccination programme.
                                                       This concern was based on case stories that
                                                       were disseminated by sensationalist media
                                                       coverage and social media platforms.

12                                                                                                                                                                                                              13
Annual Congress 2019 The Danish Microbiological Society - 11 NOVEMBER 2019 COPENHAGEN DENMARK - dms.dk
Speakers’ abstracts
[O04] PROJECT SOILTRACKER -                        [O05] ATMOSPHERIC MICROORGAN-                                                                           [O06] PREVENTING DISEASE AND
SOLVING CRIMES WITH SOIL MICRO-                    ISMS IN THE ARCTIC                                                                                      ANTIMICROBIAL USE IN ANIMALS
BIODIVERSITY                                                                                                                                               BY VACCINATION
                                                   Tina Šantl-Temkiv1, IPG Marshall2, C Mi-
Tobias Guldberg Frøslev1                           gnani3, M Alsved4, D Beddows5, M Lever2, P                                                              Anders Miki Bojesen1
                                                   Starnawski2, J Löndahl4, U Gosewinkel6, R
1
 Terrestrial Ecology, University of Copenhagen,    Lange6, A Massling6, H Wex7, K Finster8                                                                 1
                                                                                                                                                            University of Copenhagen, Department of
Denmark                                                                                                                                                    Veterinary Disease Biology, Frederiksberg C,
                                                   1
                                                    Aarhus University, Stellar Astrophysics Centre,                                                        Denmark
                                                   Department of Physics and Astronomy,
                                                   Dept. of Bioscience, Aarhus, Denmark
                                                   2
                                                     Aarhus University, Centre for Geomicrobiology,    particles, (iii) community composition of total     Bacteria are common causes of disease in ani-
                                                   Aarhus, Denmark                                     and active bacterial communities using next         mals kept for food production. For more than a
                                                   3
                                                     University of Basel, Department of Environmen-    generation sequencing of 16S rRNA molecules         100 years prevention of disease by vaccination
DNA extracted from environmental samples           tal Sciences, Basel, Switzerland                    and genes. Selected results of these studies will   has been an option employed to increase animal
(eDNA) – especially soil – is beginning to find    4
                                                     Lund University, Faculty of Engineering, Lund,    be presented that reveal latitudinal and sea-       welfare and production yield. With the advent of
use in forensic case work. It has been estab-      Sweden                                              sonal trends in the concentrations of airborne      antimicrobials prevention of bacterial infections
lished that using eDNA metabarcoding to de-        5
                                                     School of Geography, Earth and Environmental      bacteria, their predominant terrestrial and ma-     by vaccination was significantly less popular
rive “biologigal fingerprints” of soils can be     Sciences, University of Birmingham, Birmingham,     rine sources and their airborne activity, in par-   yet with the rise of antimicrobial resistance re-
used for matching of soil samples. the same        United Kingdom                                      ticular in relation to their climatic relevance.    newed interest has been placed in research and
kind of data is beginning to be used in prov-      6
                                                     Aarhus University, Department of Environmental                                                        development of vaccines targeting bacteria.
enance prediction of unknown samples. As           Science, Roskilde, Denmark                          Key words: airborne bacteria – atmospheric
part of the SoilTracker project we evaluated the   7
                                                     Leibniz Institute for Tropospheric Research,      dispersal – Arctic atmosphere – bacterial ac-       Two major challenges to veterinary vaccine
performance of different molecular marker for      Leipzig, Germany                                    tivity –biogenic ice nucleating particles           development include a considerable antigen-
provenancing soil traces of unknown origin.        8
                                                     Aarhus University, Department of Bioscience,                                                          ic variation within individual bacterial species
We tested the performance of eDNA metabar-         Microbiology Section, Aarhus University,            References                                          and a requirement of a very low production
coding of different genetic markers to predict     Stellar Astrophysics Centre, Department of Phys-    1. Šantl-Temkiv, T. et al. Bioaerosol Field Meas-   cost in order to allow an economically viable
the origin of unknown soil samples, and also to    ics and Astronomy, Aarhus, Denmark                  urements: Challenges and Perspectives in            product. Regarding antigenic variation many
predict habitat characteristics (forest, meadow,                                                       Outdoor Studies. Aerosol Sci. Technol. (2019). do   animal pathogens contain from a few to sever-
agriculture, etc..) of the sample origin. Over-                                                        i:10.1080/02786826.2019.1676395                     al hundred serotypes, each of which in princi-
all, we achieved good predictions of environ-      Arctic is affected by several climatic feedback     2. Šantl-Temkiv, T. et al. A high volume im-        ple would require an individual vaccine to en-
mental gradients and habitat classes to derive     processes, caused by changes in albedo, sea-        pinger for the study of concentration, viabili-     sure protection against disease. For this reason
a relatively accurate description of the “crime    ice extent, ice-sheet melt, and glacial retreats,   ty, activity, and ice nucleation activity of air-   the practical implementation of vaccination at
scene”. Thus, we conclude, that there is a large   which have an impact on the Arctic radiation        borne microorganisms. Environ. Sci. Technol.        farm level has been focused on the most prev-
potential for the use of metabarcoding prove-      budget. Microorganisms dispersing through           51, 11224–11234 (2017).                             alent serotypes knowing that other types could
nancing of soil trace samples.                     the atmosphere can affect some of these pro-        3. Šantl-Temkiv, T., Gosewinkel, U., Starnawski,    represent a risk. Current vaccine research is
                                                   cesses, either by their role in cloud formation     P., Lever, M. & Finster, K. Aeolian dispersal of    therefore highly aware of the need for broad
                                                   or by changing the albedo, and consequently         bacteria in southwest Greenland: their sources,     scope vaccines that cover broader than just
                                                   the melt processes of icy surfaces. In addition,    abundance, diversity and physiological states.      one or a few serotypes. As profit margins in the
                                                   melting of terrestrial ice opens up new envi-       Fems Micriobiology Ecol. 94, 1–10 (2018).           animal production generally are very small any
                                                   ronments for microbial colonization and suc-        4. Šantl-Temkiv, T. et al. Biogenic Sources of      intervention like vaccination need to be cheap
                                                   cession. For all these reasons, the Arctic is of    Ice Nucleation Particles at the High Arctic Site    to be justified economically. This not only ap-
                                                   special interest for aeromicrobiology1. We per-     Villum Research Station. Environ. Sci. Technol.     plies to the cost of the actual vaccines but also
                                                   formed several field campaigns at different lo-     53, 10580–10590 (2019).                             the cost of administering the vaccine. Having
                                                   cations in the Arctic, i.e. Nuuk Basic Research                                                         to inject a vaccine into each and every animal
                                                   Station, Villum Research Station and during a                                                           is not an option e.g. in a poultry flock of 50.000
                                                   cruise in Baffin Bay to investigate bacterial di-                                                       individuals. Technological advances allowing
                                                   versity, sources and environmental impacts of                                                           mass administration of vaccines while secur-
                                                   airborne microbial communities. We collected                                                            ing proper stimulation of a protective immune
                                                   air samples with high-volume impingers2 and                                                             response is thus a very important requirement.
                                                   on filters, precipitation samples and potential
                                                   local terrestrial and marine source samples3,4.                                                         Finally, the trend of moving production animals
                                                   Samples were investigated for (i) bacterial con-                                                        back to their natural habitat as happens in free-
                                                   centrations using cell counts and qPCR target-                                                          range or organic production systems re-intro-
                                                   ing 16S rRNA genes, (ii) concentration of total                                                         duce a much broader spectrum of disease caus-
                                                   and proteinaceous ice-nucleation                                                                        ing microorganism including bacteria, demands
                                                                                                                                                           a considerably more elaborate prevention effort.

14                                                                                                                                                                                                        15
Annual Congress 2019 The Danish Microbiological Society - 11 NOVEMBER 2019 COPENHAGEN DENMARK - dms.dk
Speakers’ abstracts
[O07] USING VIRUSES TO FIGHT                        [O08] ADJUVANTS: FROM IMMU-                        [O09] PREDATORY BACTERIA IN SOIL:   [O10] SIGNIFICANCE OF
VIRUSES – PERSPECTIVES FOR                          NOLOGIST’S DIRTY LITTLE SECRET                     KEY-STONE TAXA OF MICROBIAL         MICROPREDATOR DIVERSITY IN THE
FUTURE VACCINES                                     TO VACCINOLOGIST’S REQUISITE                       FOODWEBS?                           RHIZOSPHERE
                                                    FOR SUCCESS
Allan Randrup Thomsen1                                                                                 Tim Urich1                          Flemming Ekelund1
                                                    Dennis Christensen  1

1
  University of Copenhagen, Department of                                                                                                  University of Copenhagen, Denmark
                                                                                                                                           1

Immunology and Microbiology, Copenhagen,            Statens Serum Institute, Copenhagen S,
                                                    1

Denmark                                             Denmark

Most current vaccines work through the in-          Novel vaccine strategies include the so-called                                         While food web models of aboveground eco-
duction of humoral immune responses result-         subunit vaccines, which encompass only the                                             systems normally operate with well-defined
ing in the production of protective antibodies.     part of the pathogen to which immune rec-                                              species with well-defined characteristics; and
However, protection against a number of im-         ognition results in protection. The high pu-                                           include terms such as generalists and special-
portant human infections like HIV and hepati-       rity of these vaccines make adverse events                                             ists, belowground models assemble organ-
tis C also require the generation of cell-mediat-   less likely, but it also makes the vaccines less                                       isms in broad trophic groups; e.g. bacteria,
ed immunity; even in the case a viral infection     immunogenic and therefore potentially less                                             protozoa, nematodes. The vast taxon richness
like flu, a cell-mediated immune response is        effective. Vaccine adjuvants that increase and                                         belowground is most likely a major reason
likely to improve protection. We therefore need     modulate the immunogenicity of the vaccine                                             for this difference. Still, we would probably
vaccines, which are capable of inducing potent      are therefore added to solve this problem. Be-                                         achieve a much better understanding of the
and long-standing T-cell mediated immuni-           sides aluminum salts, which have been used                                             dynamics and functioning of the belowground
ty. While live attenuated vaccines will do that,    in vaccines for 90 years, a number of novel                                            network if we acknowledged that bacteria,
newer split vaccines are generally not efficient    vaccine adjuvants include delivery systems                                             protozoa, nematodes etc. cannot satisfactorily
in raising efficient cell-mediated immunity.        like liposomes and emulsions have been in-                                             be described in this simplistic way and that, for
Returning to our original roots, the next gener-    cluded in licensed vaccines over the last 30                                           example, the generalist vs. specialist concept
ation of vaccines are therefor likely to involve    years. However trial-and-error has been the                                            also is relevant when describing underground
various vector based, but replication impaired      explorative approach of choice, for the de-                                            systems. I will present a few examples that sup-
vaccines that mimic the antigen presentation        sign of novel vaccine adjuvants due to major                                           port this point of view and argue that future re-
associated with live bacteria or viruses. Exam-     gaps in the knowledge about immunological                                              search that follow this road will be fruitful. In
ples of this line of thinking will be presented.    activation processes. Increasing insight into                                          particular, I suggest to search for bacterial spe-
                                                    immunological mechanisms and how to ma-                                                cialist and generalist predators from soil, using
                                                    nipulate them has replaced empirical with ra-                                          largely inedible and largely edible bacteria as
                                                    tional design of adjuvants, leading to vaccine                                         bait, and to test whether presence of certain
                                                    adjuvants with increased and customized im-                                            specialist predators may provide more diverse
                                                    munogenicity profiles without compromising                                             and functionally superior bacterial communi-
                                                    vaccine safety. I will present an overview of                                          ties.
                                                    where vaccine adjuvant research is today.

16                                                                                                                                                                                        17
Annual Congress 2019 The Danish Microbiological Society - 11 NOVEMBER 2019 COPENHAGEN DENMARK - dms.dk
Speakers’ abstracts
[O11] PATTERNS OF MICROBIOME                        [O12] MICROBES INSIDE AND OUT                                                                            [O13] BINNING MICROBIAL GE-
PREDATOR COMMUNITIES AT A                                                                                                                                    NOMES USING DEEP LEARNING
GLOBAL SCALE                                        Daniel McDonald1, Justin Shaffer2, Gail Ack-
                                                    ermann2, Yan He3, Hongwei Zhou3, Junjun                                                                  Jakob Nybo Nissen1, Joachim Johansen2,
Stefan Geisen1                                      Wang4, Gabriela Sheets5, Jordan Bisanz6,                                                                 Rosa Lundbye Allesøe2, Casper Kaae Sønder-
                                                    Alexandra Obregon-Tito7, Gregor Reid8, Maria                                                             by3,
1
  Netherlands Institute of Ecology (Nioo-Knaw),     Gloria Dominguez-Bello9, Emily Vogtmann10,                                                               Jose Juan Almagro Armenteros4, Christopher
Wageningen, Netherlands                             Rashmi Sinha10, Global Microbiome Con-                                                                   Heje Grønbech3, Henrik Bjørn Nielsen5,
                                                    servancy consortium11, Mathilde Poyet12,                                                                 Thomas Nordahl Petersen6, Ole Winther7,
                                                    Mathieu Groussin12, Eric Alm12, Edgar Diaz13,                                                            Simon Rasmussen8
                                                    Rob Knight14
The microbiome has a key role in host perfor-                                                             Populations differ in host genetics, diet, life-   1
                                                                                                                                                               Department of Health Technology, Technical
mance and in environmental functioning. We          1
                                                     Uc San Diego, San Diego, United States               style and environment. Within that back-           University of Denmark, Novo Nordisk Foundation
are increasingly understanding the processes        2
                                                      Department of Pediatrics, University of Califor-    ground, unattributed factors drive variation in    Center for Protein Research, Faculty of Health
that structure microbiomes with profound ef-        nia, San Diego, California, United States             the gut microbiome. Here, we bring together        and Medical Sciences, University of Copenhagen,
forts in the last years being conducted in soils.   3
                                                      Microbiome Medicine Center, Division of Lab-        data from The Microsetta Initiative and the        Denmark
Most studies focused on bacteria and fungi,         oratory Medicine, Zhujiang Hospital, Southern         Global Microbiome Conservancy, projects            2
                                                                                                                                                               Novo Nordisk Foundation Center for Protein
the primary drivers of many processes in soils.     Medical University, Guangzhou, China                  which spans multiple populations, and inte-        Research, Faculty of Health and Medical Sciences,
Yet, we lack an understanding of larger mi-         4
                                                      Ministry of Agriculture Feed Industry Centre,       grate these data in a meta-analysis with other     University of Copenhagen, Copenhagen, Denmark
crobiome components, while often ignoring           College of Animal Science and Technology              population datasets that used the Earth Micro-     3
                                                                                                                                                               Bioinformatics Centre, Department of Biology,
(trophic) interactions that likely contribute in    China                                                 biome Project protocols. The combined anal-        University of Copenhagen, Copenhagen, Denmark
structuring soil microbiomes. Here I will show      Agricultural University, Beijing, China               ysis spans over 30,000 human samples. Spe-         4
                                                                                                                                                               Department of Health Technology, Technical
the recent advances that help in filling these      5
                                                      Department of Anthropology, Emory College of        cifically, 16S rRNA V4 amplicon data from The      University of Denmark, Kongens Lyngby, Denmark
knowledge gaps. I will highlight work on pro-       Arts and Sciences, Atlanta, Georgia, United States    Microsetta Initiative and other studies using      5
                                                                                                                                                               Center for Genomic Medicine, Copenhagen
tists and nematodes, both representing the key      6
                                                      Hooper Foundation, University of California, San    common molecular protocols were integrated         University Hospital, Copenhagen, Denmark
predators of bacteria and fungi and as such         Francisco, United States                              using Qiita. These data recapitulate and extend    6
                                                                                                                                                               National Food Institute, Technical University of
likely are important in structuring the microbi-    7
                                                     Department of Anthropology, University of            Yatsunenko et al. (2012, Nature), showing that     Denmark, Kongens Lyngby, Denmark
ome. After giving an overview on these groups       Oklahoma, Oklahoma, United States                     European, Australian, and North American           7
                                                                                                                                                               Bioinformatics Centre, Department of Biolo-
of organisms, I will show their functional im-      8
                                                      Centre for Human Microbiome and Probiot-            populations are more similar to one other than     gy, University of Copenhagen, Department of
portance and global efforts that aim at deci-       ic Research, Lawson Health Research Insti-            each is to South American or sub-Saharan           Applied Mathematics and Computer Science,
phering their community structures in soils.        tute, Department of Biochemistry and Applied          African populations. Unexpectedly, individu-       Technical University of Denmark, Center for
Together I will show that this work will help in    Mathematics, The University of Western Ontario,       als from Bangladesh, the Philippines and Iran      Genomic Medicine, Copenhagen University
furthering our knowledge on the global distri-      London, Canada                                        exhibit an intermediate relationship between       Hospital, Denmark
bution and functioning of soil biodiversity.        9
                                                      Department of Anthropology and of Bioche-           Western and non-Western groupings. These           8
                                                                                                                                                               Nnf Center for Protein Research, University of
                                                    mistry and Microbiology, Rutgers University,          data were then integrated with the Earth Mi-       Copenhagen, København N, Denmark
                                                    New Brunswick, Nj, United States                      crobiome Project, highlighting the microbial
                                                    10
                                                       Metabolic Epidemiology Branch, Division of         connection between humans and the envi-
                                                    Cancer Epidemiology and Genetics, National            ronment. Defining how populations relate is a      Despite recent advances in metagenomic
                                                    Cancer Institute, Bethesda, Maryland, United          critical step in translating microbiome results.   binning, reconstruction of microbial species
                                                    States                                                This backdrop carries medical implications, as     from metagenomics data remains a challeng-
                                                    11
                                                       Center for Microbiome Informatics and Thera-       it is presently unknown whether such massive       ing task. Here we use recent advances in deep
                                                    peutics, Department of Biological Engineering,        differences in microbial community composi-        learning to develop an algorithm that uses
                                                    Massachusetts Institute of Technology, Cam-           tion among populations have the potential to       deep variational autoencoders to encode se-
                                                    bridge, Massachusetts, United States                  modulate microbiome therapies.                     quence co-abundance and k-mer distribution
                                                    12
                                                       Center for Microbiome Informatics and Ther-                                                           information prior to clustering. We show that
                                                    apeutics, Department of Biological Engineering,                                                          our variational autoencoder is able to inte-
                                                    Massachusetts Institute of Technology, The                                                               grate these two distinct data types without any
                                                    Broad Institute of Mit and Harvard, Cambridge,                                                           prior knowledge of the datasets. Our method
                                                    Massachusetts, United States                                                                             outperforms existing state-of-the-art binners
                                                    13
                                                       Department of Pediatrics, University of Califor-                                                      on contig datasets, reconstructing 127-167%
                                                    nia, San Diego, La Jolla, California, United States                                                      as many precise and complete genomes. Ad-
                                                    14
                                                       Department of Pediatrics, University of                                                               ditionally, we developed a multi-sample and
                                                    California, Department of Computer Science &                                                             splitting strategy, that enables assembly of 23-
                                                    Engineering, Center for Microbiome Innovation,                                                           89% more strains compared to commonly used
                                                    University of California San Diego, La Jolla,                                                            single binning strategies. Furthermore, our
                                                    California, United States                                                                                method enables direct high-resolution taxo-
                                                                                                                                                             nomic profiling across samples.

18                                                                                                                                                                                                          19
Speakers’ abstracts
                                                  [O14] TRASH TO TREASURE: EXPLOR-                   [O15] ICARS-THE INTERNATIONAL                     [O16] FECAL TRANSPLANTS – THE
                                                  ING THE WASTEWATER SYSTEM                          CENTER FOR ANTIMICROBIAL                          ULTIMATIVE PROBIOTIC FOR PIGS?
                                                  WITH HIGH QUALITY MAGS FROM                        RESISTANCE SOLUTIONS
                                                  LARGE-SCALE NANOPORE DATA                                                                            Nuria Canibe1
                                                                                                     Robert Leo Skov1
                                                  Caitlin Singleton1, Martin Andersen2,                                                                Aarhus University, Tjele, Denmark
                                                                                                                                                       1

                                                  Rasmus Kirkegaard2, Thomas Michaelsen2,            SSI, Microbiology, Denmark
                                                                                                     1

                                                  Søren Karst2, Morten Dueholm2, Per Nielsen2,
                                                  Mads Albertsen2

                                                  1
                                                    Aalborg University, Centre for Microbial
Finally, to demonstrate the scalability of our    Communities, Department of Chemistry and           Despite recent global efforts to mitigate the     It is becoming increasingly evident that the gas-
method, we bin a human gut microbiome da-         Bioscience, Aalborg University, Aalborg,           impact of antimicrobial resistance (AMR), in-     trointestinal microbiota has a significant impact
taset from 1,000 samples. By employing GPU        Denmark                                            formation and evidence on the effects of AMR      on the overall health and performance of the pig.
acceleration for training and clustering we re-   2
                                                    Aalborg University                               on health and the economy have so far failed      This has led to intensified studies on the compo-
construct 30% more near-complete bins com-                                                           to be translated into actions in many parts of    sition of the gastrointestinal microbiota, factors
pared to state-of-the-art and even ensemble                                                          the world, especially in low and middle income    affecting it, and the impact of the microbiota on
strategies. Our method Variational Autoencod-     Improvements to wastewater resource re-            countries (LMICs). The International Centre for   health, growth performance, and more recently,
ers for Metagenomic Binning (VAMB) can be         covery and treatment efficiency are essential      Antimicrobial Resistance Solutions – ICARS –      behavior of the host. Pig production research has
run on standard hardware and is freely avail-     for fulfilling the UN sustainable development      is a new partnership that aims to conduct pol-    been heavily focused on assessing the effects of
able at https://github.com/RasmussenLab/          goals for both sustainable cities (goal 11) and    icy relevant applied research at international,   feed additives and dietary modifications to al-
vamb.                                             responsible production and consumption             national and local level to identify knowledge    ter or take advantage of select characteristics of
                                                  (goal 12). Microbes are central to the processes   and evidence-based solutions in close collab-     gastrointestinal microbes to improve health and
                                                  underpinning wastewater treatment, however         oration with countries and key stakeholders.      feed conversion efficiency. Research on faecal
                                                  knowledge of this complex community is lim-        ICARS will support LMICs to conduct applied       microbiota transplantation (FMT) as a possible
                                                  ited to isolates, marker genes or incomplete       research relevant to their local challenges       tool to improve outcomes in pigs through ma-
                                                  genomes. To improve our capacity to charac-        and therefore implementing aspects of their       nipulation of the gastrointestinal microbiome is
                                                  terise this community at the genome level, we      AMR National Action Plans (NAPs). It will aim     very recent and limited data is available. Results
                                                  conducted large-scale long-read sequencing         to bridge the gap between science and poli-       on FMT in humans demonstrating the transfer
                                                  of 23 Danish full-scale wastewater treatment       cy translating national action plans into evi-    of phenotypic traits from donors to recipients
                                                  plant (WWTP) metagenomes using the Nano-           dence based practices on the ground. Projects     and the high efficacy of FMT to treat Clostridium
                                                  pore PromethION platform, producing >1 Tbp         and activities will aim to build local capacity   difficile infections in humans, together with data
                                                  (or 17 TB raw) of long-read data. These data       and capability to sustain and scale up the ev-    from pigs relating gut microbiota composition
                                                  enabled the recovery of 987 metagenome-as-         idence-based solutions identified. Output will    with growth performance has likely played an
                                                  sembled genomes (MAGs), including 36 cir-          be made accessible and where appropriate in       important role in the interest towards this strate-
                                                  cular genomes, after short-read data polishing     open repositories. ICARS will work interna-       gy in pig production.
                                                  (>0.9 Tbp Illumina). The recovered MAGs met        tionally as a solution development partner-
                                                  stringent high-quality draft requirements of       ship with projects and activities in various      There are obvious inherent biosecurity and
                                                  >90% completeness,
Speakers’ abstracts
[O17] LOCAL DRUG DELIVERY TO                                                                               [O18] MICROBIOLOGICAL AND                          [O19] TUBERCULOSIS–DIAGNOSIS
TARGET BIOFILM INFECTIONS                                                                                  PATHOGENESIS FEATURES DISTIN-                      FROM A LABORATORY POINT OF
                                                                                                           GUISHING LYME DISEASE AND                          VIEW
Rikke Louise Meyer1, Raoul Walter2, Sig-                                                                   RELAPSING FEVER SPIROCHETES
ne Maria Nielsen2, Pernille Ommen2, Line                                                                                                                      Dorte B. Folkvardsen1
Hansen2,                                                                                                   Sven Bergstrom1
Hieu Quang3, Jørgen Kjems2, Alexander Ze-                                                                                                                     1
                                                                                                                                                               Statens Serum Institut, International Reference
likin4, Rikke Christiansen5                                                                                Molecular Biology, Umeå University, Umeå,
                                                                                                           1
                                                                                                                                                              Laboratory of Mycobacteriology, Copenhagen S,
                                                                                                           Sweden                                             Denmark
1
  Interdiciplinary Nanoscience Centre (Inano),
Faculty of Science and Technology, Aarhus                                                                  The recent proposal of splitting the genus
University, Aarhus, Denmark                           Aptamer-targeted liposomes accumulated in                                                               Mycobacteria are a diverse group of actino-
                                                                                                           Borrelia into two genera in the newly formed
2
  Interdisciplinary Nanoscience Center (Inano),       S. aureus biofilms, resulting in eradication of                                                         bacteria, comprising more than 100 species.
                                                                                                           family of Borreliaceae, i.e. Borrelia and
Aarhus University, Aarhus, Denmark                    biofilms in vitro, while non-targeted lipos-                                                            The most well-known and well characterized,
                                                                                                           Borreliella has motivated us to reflect upon
3
  Aarhus University, Denmark                          omes were less effective. Although this result is                                                       Mycobacterium tuberculosis (Mtb), is the caus-
                                                                                                           how these organisms has been characterized
4
  Department of Chemistry, Aarhus University,         promising, the burst release offers little control                                                      ative agent of tuberculosis (TB). Other species
                                                                                                           and differentiated. Herein we therefore aim to
Aarhus C                                              over dosage and exposure time. We therefore                                                             are Mycobacterium leprae, causing leprosy and
                                                                                                           take a closer look on the biology and virulence
5
  Interdisciplinary Nanoscience Center (Inano),       proceeded with prodrug therapy. The embed-                                                              non-tuberculous mycobacteria (NTM) that
                                                                                                           attributes of the two suggested genera, i.e.
Aarhus University, Denmark                            ded enzyme continuously converted the prod-                                                             are common in the environment and some of
                                                                                                           those causing Lyme borreliosis and relapsing
                                                      rug to moxifloxacin at the implant surface,                                                             them are opportunistic pathogens.
                                                                                                           fever borreliosis, respectively.
                                                      which prevented biofilm formation, even un-
Antibiotic treatment of biofilm infections often      der flow.                                                                                               It is not an easy group of bacteria to culture,
fail because the dose that can be administered                                                             In fact, both these genera has a lot in common     ranging from a few days to positive culture for
safely is insufficient to eradicate the biofilm. We   Targeted drug delivery and pro-drug therapy          with similar infection biology features . They     the NTM fast growers, to 56 days until a nega-
hypothesize that treatment will be more effec-        enable local antibiotic therapy. Drug the ex-        are both characterised as bacterial zoonoses,      tive culture for the majority, to the almost-im-
tive if drugs are delivered or synthesized at the     posure time and concentration is better con-         transmitted by hematophagous arthropods            possible-to-grow Mycobacterium leprea.
site of infection, leading to a high local con-       trolled in prodrug therapy, and we believe that      with almost identical microbiological appear-      Tuberculosis is the number one cause of death
centration with minimal side effects. We aimed        our encouraging results will pave the way for        ance. Nevertheless, a closer look at their gen-    related to an infectious disease, recently it has
at delivering a high local dose of antibiotics        implementing more potent drugs that target           otypic and phenotypic characteristics clearly      surpassed HIV/AIDS.
through two different approaches: i) targeted         persister cells in treatment of biofilm infec-       reveals several differences that might moti-
delivery of encapsulated antibiotics, and ii) lo-     tions.                                               vate the suggested split. On the other hand, a     The quest for the fastest way to diagnose TB
cal drug synthesis through prodrug therapy.                                                                change of this well-established classification     in the lab is still ongoing, and a number of
                                                                                                           within the genus Borrelia might impose an          molecular tests to rapid detection in primary
Targeted drug delivery uses drug encapsulation,                                                            economical burden as well as a great confusion     specimens have been developed. However,
accumulation in the biofilm, and a triggered                                                               in the society, including medical and scientific   the sensitivity is still lower than for culture,
burst release. We encapsulated vancomycin and                                                              societies as well as the general population.       which besides taking several weeks, requires
rifampicin in temperature-sensitive liposomes                                                                                                                 expensive equipment, specialized media, and
decorated with aptamers that bind Staphylococ-                                                                                                                skilled personnel. A big portion of the analyzes
cus aureus. Accumulation, drug release, and kill                                                                                                              applied to TB specimens, are still delayed until
efficiency was quantified on S. aureus biofilms.                                                                                                              sufficient growth of an isolated culture is ob-
                                                                                                                                                              tained.
Prodrug therapy uses immobilized enzymes to
convert non-toxic prodrugs to the active drug.                                                                                                                Mtb is an enigma -a slow growing, not very
We developed a novel method for synthesizing                                                                                                                  contagious, hard to culture bug, with huge
glucuronide prodrugs, opening the door for an-                                                                                                                success worldwide.
timicrobial prodrug therapy. We immobilized
the catalyst (β-glucuronidase) in a layer-by-lay-
er coating on titanium implants. Glucuronide
prodrugs of moxifloxacin was administered in
solution, and the effect on S. aureus viability
and biofilm formation was quantified.

22                                                                                                                                                                                                          23
Speakers’ abstracts                                 Keynote abstract
[O20] SLOW-GROWTH AND                               [O21] COOPERATION AND COMPE-
NO-GROWTH IN NATURAL                                TITION IN BACTERIA: FROM MODEL
ENVIRONMENT                                         SYSTEMS TO THE MICROBIOME

Hans Røy1                                           Kevin Foster1

1
  Institute for Bioscience, Aarhus University,      1
                                                        University of Oxford, Oxford, United Kingdom
Aarhus C, Denmark

Research within the ocean drilling projects         Microbes display a dizzying array of social
have demonstrated large populations of              traits, from enzymes released to break down
prokaryotes that live deep below the seafloor       nutrients and antibiotics, through slimey se-
under extreme energy limitation. These or-          cretions that protect and disperse, to draconi-
ganisms are not merely the dying remains of         an molecular machines that stab, rupture and
the once thriving surface community, but cells      poison their competitors. But what determines
that live in a delicate balance with the flux of    whether microbes cooperate or compete with
energy that can be harvested from slow degra-       each other, and how does this affect their
dation of ancient organic material. The com-        hosts? To answer these questions, we combine
munity composition is distinctively different       theory and experiments with pathogenic bac-
from that of the energy rich and dynamic sed-       teria and the mammalian microbiome. This
iment surface, but the transition between the       has revealed that clonemate patches naturally
two occur only 10 cm below the seafloor. Close      emerge in microbial communities, which fa-
examination of the ratio between community          vours strong cooperation by kin selection. But
size and community respiration confirms that        interactions between strains and species are
the energy available for the individual organ-      often competitive. We find that bacteria are of-
isms this close to the seafloor is just as meager   ten at war and are even capable of reciproca-
as for those that have been buried for millions     tion, detecting incoming attacks and respond-
of years without resupply of organic substrates.    ing collectively in devastating counterattacks.
This demonstrate a life strategy linked to cellu-   Microbial interactions follow the same evolu-
lar metabolism far below what we know from          tionary principles that were first understood
laboratory cultures, which is still distinct from   through the study of animal behavior. Howev-
dormancy. This this type of organisms are not       er, one fascinating property of microbes is that
restricted to exotic niches buried deeply below     their entire ecosystem can lie within another
the seafloor. They can be studied with relative     evolving organism – a host - that is trying to
ease in sedimentary environments where they         control them and their interactions. The result
constitute a dominating fraction of the com-        appears to be a complex coevolutionary dance
munity. In environments where they co-exist         with the host and its immune system on one
with fast growing types, however, their activity    side, and the whole microbiota on the other.
may easily escape detection.

24                                                                                                     25
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