Contents of antinutritional factors in different faba bean cultivars - @scenoprot
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Hemp seed processing Fermented quinoa Phytochemicals and and buckwheat antinutrients of protein rich plants Enzymatic treatment Fermentation using Analysis of Oil removal different lactic acid antinutrient and Germination bacteria strains phytochemical levels from faba bean, lupin, rapeseed press cake, hemp seed, buckwheat and Evaluation of protein Protein digestion quinoa solubility and other and availability features analysis by using Infogest method Results from different plants Development of several concept published in: products “Contents of phytochemicals and Product development antinutritional factors in commercial protein-rich plant products” 12.3.2021 2
• Seven faba bean cultivars • Three collection points • Vicine/convicine, trypsin inhibition, condensed tannins, lectins and phytic acid 12.3.2021 3
Antinutrients Vicine and convicine Trypsin inhibitors Condensed tannins toxic to the persons suffering can limit protein availibility. can have positive and negative from genetic loss of one Especially when faba bean used effects to the health. enzyme. Otherwise non-toxic. as feed. Compromise intake of proteins, polysaccharides and metal ions. Can inhibit bacterial growth and thus ruminal digestion. Lectins Phytic acid can cause food poisoning like chelates metal ions like iron and symptoms when consumed high calcium making them amounts. unavailable. 12.3.2021 4
Vicine and convicine • Tiffany is known low level variety. Genetical background known. • Vroma also had low levels of vi/co when fully maturated • Gloria had relatively low level of convicine when fully maturated. • Young seeds contain more than maturated 12.3.2021 5
Trypsin inhibition • Age do not effect • Overall, Tiffany and Julia shows strongest trypsin inhibition, Talia and Gloria mildest. 12.3.2021 6
Condensed tannins • Talia and Gloria are zero-tannin varieties (white flower) • Tiffany and Julia contain most when seeds are young • In line with the trypsin inhibition results 12.3.2021 7
Lectins • Lectins were analysed by mixing faba bean extract with red blood cells • Differences are more related to cultivar than age • Sampo and Julia contained the most • Least lectin was in Vroma 12.3.2021 8
Phytic acid Phytic acid levels are low when seeds are young as expected. No significant differences between varieties 12.3.2021 9
26.7 Oligosaccharides 100 Oligosaccharides causes 50 • stomach pain 0 Sampo Louhi Gloria Vroma Tiffany Talia Julia • Some interesting cultivars, Glucose Sucrose Fructose like Louhi when fully Raffinose Stachyose Verbascose developed • Green beans are rich in 2.8 16.8 sucrose 50 40 30 20 10 0 Sampo Louhi Gloria Vroma Tiffany Talia Julia Gloria Vroma Tiffany Talia Julia 12.3.2021 10
11 Feed preservation reduce antinutrients Vicine and convicine nearly disappear - 100 with fermentation % Reduction of tannins Variety: Kontu Rinne et. al. 2020: Fermentation quality of ensiled crimped faba beans using different additives with special attention to changes in bioactive compound - 82 % - 29 %
Conclusions • Tiffany is safe for vicine/convicine regard • Talia and Gloria could be potential for animal feed since low tannin and trypsin inhibition levels. • Vroma contained lowest level of lectin. However safe level for lectin is not known. • Phytic acid levels are at its lowest when beans are used young and green. 12.3.2021 12
Jarkko Hellström Juha-Matti Pihlava Minna Kahala Pirjo Mattila Anne Pihlanto Academy of Finland Thank you! Apetit Oyj www.luke.fi/scenoprot @scenoprot
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