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CELL CULTURE
CATALOGUE
Classical Media
Special Media
Salt Solutions
Buffers
Reagents
Supplements
Sera
Technical Information
CELL CULTURE CATALOGUE
Paradiesrain 14
Postfach 427
CH-4123 Allschwil 1
Switzerland
Tel. +41 (0)61 486 80 80
Fax +41 (0)61 486 80 00
info@bioconcept.ch
www.bioconcept.ch
Dear Customer
We are proud to present our catalogue, which provides you
with a concise overview of our company and our products.
For our catalogue, we have chosen “life in the ocean” as the
theme. The oceans were the original incubator of life and
provide a complex and delicate balance, which supports the
diversity of life. With our cell culture products, BioConcept Ltd.
seek to emulate nature by providing the optimal medium to
support cell cultivation.
Thank you for using our products and services,
BioConcept Ltd.
Martin Howald
CEO / President
1
Who we are
BioConcept Ltd. is
supplying high-tech tools
and products to the
biological research
community since 1978.
We are a dedicated
system supplier, providing
our customers with a
comprehensive product
portfolio and customized
solutions.
3
Going
Austria Latvia
Czech Republic Lithuania
Iceland Denmark Netherlands
ahead
Estonia Poland
Finland Portugal
Germany Slovakia
Hungary Sweden
Canada Italy China
USA
India
Sub-Saharan Africa Japan
Singapore
Taiwan
Over the years we have become
a leading supplier and service 65 %
Sales international
partner for numerous respected
pharmaceutical and academic
institutions in Switzerland and
around the world.
2018
With our new facilities, and
our convenient location near
Basel airport, we aspire to 5%
expand further internationally Sales international
while also serving local
biological researchers. We
shall continue to listen to the 35 %
needs of the market and be Sales Switzerland
flexible when meeting them.
1995
95 %
Sales Switzerland 5
4
Construction and Move to today’s location
Foundation of Flow completion of Liquid I at Paradiesrain 14, Construction
Laboratories AG and Powder I Allschwil, Switzerland of Liquid II
1978 1992 1993 1995 2006 2009 2015 2017
Change of name Implementation Construction Revision
into BioConcept Ltd. of the ISO 9001 of Powder II of Liquid I
and acquisition of norm for the whole
Looking
AMIMED Produkte AG company
back Giving
At BioConcept Ltd. we are proud
of the progress we have made since back
our foundation in 1978.
We believe that we can
We have been producing within a certified
only operate well in
quality management system since 1995
and our new cell culture and sterile liquid
a healthy society and
production plant has vastly improved our environment.
already high standards. BioConcept Ltd.’s
expansion into the tissue culture market
in 1992 allowed us to meet the needs of the
sophisticated and evolving pharmaceutical Hence we commit
and bio-pharmaceutical markets. ourselves to support
various cultural and
educational programs.
6 7
Support of the Swiss research community Jazz and Blues Events, Marabu Blues Night
PhD- and postdoctoral retreats are events aiming Built as a school, turned into a cinema and now a
to foster scientific exchange and progress. cultural institution in Gelterkinden, the Marabu
As scientific areas are no longer stand alone has a long and fascinating history and a unique
research fields and interdisciplinary research is ambience. Today, the independent non-profit
the new gold standard, such retreats encourage organization Kultur Marabu Gelterkinden puts
the collaboration between the PhD students and together a diverse program and the annual
postdoctoral Fellows. They have the opportunity Blues Night is definitely a highlight in the agenda.
to share their work, talk about science and build The live music event is dedicated to promoting
new relationships. blues music in Switzerland and presents local bands.
Ozeanium Basel Frauenplus
At the Ozeanium, which will be part of the Basel Frauenplus Baselland is an independent non-profit
Zoo, visitors dive into fascinating underwater women’s organization dedicated to helping
worlds and learn more about the complex blue women and their families in difficult situations.
ecosystem that covers our planet. On an area The organization’s objective is to alleviate
of approximately 10 000 m2, 40 aquariums hardship fast and in a straightforward manner.
accommodate sharks, rays, penguins, corals
and many more species in over 4.6 Mio. litres
of water. Several thousands of animals from
all over the globe convey the size of the ocean,
its diversity, its beauty, but also its fragility and
its threats. The entire Ozeanium revolves around
the subject of resources and sustainability,
turning its audience into experts for a world that
needs to be valued and protected. 1
Tembea
Tembea is the name of the new elephant
enclosure in the Basel Zoo. After just over three
years of construction, it opened its doors
for the public in 2017. At over 5 000 m2, the new
facility is two and a half times the size of the
old one. The public can view the elephants in the
open enclosure and the near-natural savannah 1
landscape. In addition to African elephants,
harvester ants, brown rats and a few species
of fish live in the Elephant house and the
enclosure provides nesting areas for birds and
accommodation for bats. 2
Isele
In Isele, a small village in East African Tanzania,
a new village centre is built with the help
of several sponsors. An administration building
2
with offices and a meeting room, such as a
shop and an Internet café are already completed.
Furthermore, there will be an economy building
with workshops and a corn mill will complement
the new compound. Thus, on the one hand,
economic activities are promoted and, on the
other hand, already existing organizations
and social structures are consolidated locally.
In the future, further buildings are to be built
in the surrounding area and incorporated into a
village structure. 3 3
8
About
BioConcept Ltd.
BioConcept Ltd.
About
3 – 9
Classical
Classical Media
Media
11 – 41
Special
Special Media
Media
43 – 67
Salt Solutions / Buffers /
Buffers / Reagents
& Supplements
Salt Solutions /
Reagents & Supplements
69 – 87
Sera
89 – 95 Sera
Technical Information /
Information /
Protocols
Protocols
Technical
97 – 123
Index
Index
125 – 132
About
BioConcept Ltd.
BioConcept Ltd.
About
About us
Infrastructure
Products
Great Barrier reef
The great barrier reef, located north east of
Australia, contains an immense variety of life. It is
inhabited by 400 types of coral and 1 500
known species of fish, which is around 10 % of all
fish species in the world. The coral system is in
fact made up of 2 900 individual reefs, covers an
area of 344 400 square meters, and is the world’s
largest living structure. Sadly, it is currently facing
some of the greatest threats in its life span, as
recent heatwaves have bleached 90 % of the corals
in some places. There is some hope, however.
Scientists around the world are constantly finding
new ways to support the reef and help it recover.
Furthermore, the Australian government has
recently unveiled a £60 million protection plan for
the reef as a response to its current problems.
2 3
About us
BioConcept Ltd.
About
BioConcept Ltd. is a privately owned company with more
than 40 years of experience in serving the biological
community. We strive to combine this experience with an
innovative spirit to support our customer’s needs with
the best possible solutions.
To do so, BioConcept Ltd. establishes close relationships with its customers to be
able to respond to their wishes. To guarantee that our products are of the highest
standard, BioConcept Ltd. has been producing within a certified quality management
system (ISO 9001) since 1995. The current ISO standard is 9001:2015. Our SQS
certificate can be downloaded at www.bioconcept.ch.
At BioConcept Ltd. we are proud of our dynamic team and the available know-how we
like to share with our partners. We are happy to move on together.
Please contact our specialists if you would like
to discuss possibilities in further detail:
Tel. +41 (0)61 486 80 80
info@bioconcept.ch
4 5
1
Infrastructure
BioConcept Ltd.
About
BioConcept Ltd. is currently running two independent liquid
media production facilities (Liquid I and Liquid II) and two
independent powder media production facilities (Powder I
and Powder II).
2 Both are equipped with qualified ISO 5 (GMP A) cleanrooms. Liquid I is used for
production of special media and small batch sizes from 5 L up to 1 500 L. With
Liquid I we are able to handle a broad range of container sizes from 1 ml up to 500 L,
1 The 5 000 L state-of-the-art media
tank is linked to a cleaning-
giving us a great deal of flexibility to fulfil our customers requests. Liquid II is the fully
and sterilization-in-place system automated production facility of BioConcept Ltd., which went online in 2015. It is
(CIP/SIP system). exclusively used for Animal Component Free (ACF) production. The plant has been
2 Powder II with a batch size up designed to maximize efficiency, sustainability and productivity. Its layout as well
to 800 kg. as its equipment is in accordance with GMP guidelines, e.g. air and water preparation
3 Liquid I filling line for special facility, calibrated and qualified instruments, validated processes, fully integrated
media in the ISO 5 cleanroom. Standard Operating Procedures (SOPs) and comprehensive documentation of
4 In-house QC (Quality Control) production. The state-of-the-art air processing system provides us with the optimal
ensures high level of quality. conditions required for sterile liquid production. We constantly monitor and control
the pressure, temperature, humidity and particle count in the air and we regularly
disinfect our facilities using H2O2. The water preparation facilities are specifically
engineered to efficiently generate the highest standard Water For Injection (WFI)
available. This gives us the capability to generate 5 000 L of media a day, allowing us
to meet the rising demands from our customers. For the preparation of the bottles
3 we use a cleanroom robot to minimalize the particle count. All further steps, including
sealing and labelling, are also performed automatically. In summary, the plant has
been designed to create an environment that upholds the highest degree of sterility
possible, thereby ensuring a sterile final product.
BioConcept Ltd. runs two powder media plants (Powder I and II) with a capacity
of 100 kg/day and 800 kg/day, respectively. The impact mills produce powder with
an average grain size of < 20 µm. The plants are made for batch sizes from 2 kg
up to 800 kg, which can be filled into various container sizes, in accordance to the
customers needs. The advantages for the customer of our powder plants are the
high homogeneity of the product, with a low particle size, a low risk of
cross-contamination and the easy and safe handling.
In addition to these production facilities, BioConcept Ltd. guarantees the high quality
of the products with its in-house quality control (QC). BioConcept Ltd. performs
tests for sterility, pH-value, osmolality, cell growth, endotoxin levels and conductivity.
For powder formulations we also test the bioburden. Further tests, if required, are
outsourced to certified analytical laboratories.
4
6 71
Products
BioConcept Ltd.
About
The strength and focus of BioConcept Ltd. lies in
manufacturing customized cell culture media as well as
defined media for recombinant protein production.
Besides that BioConcept Ltd. offers all standard (classical) media and solutions.
2 Furthermore, the production facilities are superbly equipped to manufacture sterile
QC liquids, microbial broths and agars. Therefore, our product line includes:
1 The robotic arm plays a crucial – Special customer-designed media
role in the automatic filling line – Contract manufacturing of sterile liquids and powder formulations
in LQII.
– Production media for CHO, Hybridoma and Insect Cells
2 The automated filling process – Individual solutions for your cell culture requirements
ensures reliable sterility.
– Complete cell systems applications for CHO cells
3 In LQII containers up to 1 000 L – Standard media
are easily handled.
– Serum-free and ACF media
– Liquid as well as powder media formulations
– Buffers and balanced salt solutions
– Supplements and auxiliary reagents
– Animal sera
In addition to the broad range of cell culture products, we can offer the highest
degree of flexibility and customization in a timely manner. Our customers have the
following options:
– Modifications of standard products
3
– New products according to customers recipes
– Outsourcing of media production
– Variable batch sizes starting from 5 L up to 5 000 L (liquid) and 2 kg up to 800 kg
(powder)
– Variable packaging sizes (1 ml up to 1 000 L) and packaging systems (PET / glass
bottles, sterile bags, customized tubing systems, as well as customer specifications)
– Sterilization through sterile filtration (0.22 µm) or hot air/vapour sterilization
Customized products can be delivered within six to eight weeks after the order has
been placed, including QC. For more detailed information please contact us at
info@bioconcept.ch.
8 9Classical
Media
Basal Medium Eagle with EBS
Dulbecco's MEM (DMEM)
Classical Media
Dulbecco's MEM Low Glucose
Dulbecco's MEM High Glucose
DMEM / Ham's F-12 (1:1)
Ham's F-10 Medium
Ham's F-12 Medium
IMDM
Leibovitz L-15 Medium
Medium 199
McCoy’s Medium
MEM Alpha
MEM EBS
MEM HBS
RPMI 1640
Waymouth's Medium
William's E Medium
Manta Ray [ Manta birostris ]
Manta Rays, also known as devil fish due to
their devilish horns, can reach the massive
width of 7 meters. Because of their size, and
general appearance, they were often feared by
sailors who believed they could sink their boats.
In 1978, however, divers in California found
the animal to be surprisingly friendly. While they
normally live alone, large groups have been
seen together during times of migration and at
points called “cleaning stations”, where the
mantas are cleaned of parasites by small fish
looking for an easy snack.
10 11Classical Media
Basal Medium Eagle with EBS
Basal Medium Eagle (BME) was originally developed for studies to determine essential
nutrient requirements of mouse “L” cells and HeLa cells in culture. This medium with
a host of modifications is widely used and supports both the primary culture of a wide
Classical Media
variety of normal mammalian and transformed cell lines. BioConcept Ltd. offers BME
liquid with either Earl's Standard formula, Hank's salts and/or stable Glutamine.
Basal Medium Eagle
Cat. No. 1-06F01-l
BME EBS
Concentration
Inorganic Salts mg / L Amino Acids mg / L
CaCI2 2H2O 265 L-Phenylalanine 16.5
KCI 400 L-Threonine 24
MgSO4 7H2O 200 L-Tryptophan 4
NaH2PO4 H2O 140 L-Tyrosine 18
NaCl 6 800 L-Valine 23.5
NaHCO3 2 220 Vitamins
Other Components myo-Inositol 1
D(+)-Glucose 1 000 (+)-Biotin 1
Phenol Red 10 D-Pantothenic acid Calcium salt 1
Amino Acids Choline chloride 1
L-Arginine HCl 21 Folic acid 1
L-Cystine 12 Nicotinamide 1
L-Histidine HCl H2O 10.81 Pyridoxal HCl 1
L-Isoleucine 26 Vitamin B2 0.1
L-Leucine 26 Vitamin B1 HCl 1
L-Lysine HCl 36.5 To be added separately
L-Methionine 7.5 L-Glutamine 292
Available Basal Medium Eagle
Cat. No Modification Size
1-06F01-l BME with EBS w/o L-Gln 500 ml
1-06F50-l BME with EBS, with stable Gln (434 mg / L) 500 ml
1-08F01-l BME with HBSS, w/o l-Gln 500 ml
1-08F50-l BME with HBSS, with stable Gln (434 mg / L) 500 ml
Other modifications are available upon request at info@bioconcept.ch.
References
1. Eagle, H. (1955) Proc. Soc. Exp. Biol. Med 89, 362 3. Eagle, W.R. (1956) Ann. NY. Acad. Sci. 63, 666
2. Eagle, H. (1955) J. Biol. Chem. 214, 839 4. Eagle, H. (1959) Science 130, 432
12 13Dulbecco's MEM (DMEM) Available Dulbecco's MEM Low Glucose Media
Dulbecco's MEM is the most widely used modification of BME. It contains four Cat. No Modification Size
times the concentration of amino acids and vitamins. Non-essential amino acids 1-25F01-I DMEM LG w/o L-Gln 500ml
and certain essential trace elements were added and the bicarbonate concentration 1-25F03-I DMEM LG with L-Gln 500ml
was increased. The standard formula is with 1 000 mg / L glucose, the “high glucose” 1-25F04-I DMEM LG w/o L-Gln, with 25 mM HEPES 500ml
variation with 4 500 mg / L. Dulbecco’s MEM was originally developed for the culture 1-25F22-I DMEM LG w/o L-Gln w/o Phenol Red 500ml
of mouse embryonic cells. Today it finds a broad application for serum free culture of 1-25F23-I DMEM LG (1.0 g / L) with stable Gln (868 mg / L), 500ml
normal and transformed mouse and chicken cells, e.g. 1:1 with Ham's F-12. w/o Phenol Red
Classical Media
1-25F24-I DMEM LG w/o L-Gln, with 25 mM HEPES, 500ml
Dulbecco's MEM Low Glucose w/o Phenol Red
1-25F50-I DMEM LG with stable Gln (868 mg / L) 500ml
Cat. No. 1-25F01-I
DMEM 1-25F51-I DMEM LG with 25 mM HEPES, 500ml
Low Glucose with stable Gln (868 mg / L)
1-25K03-I DMEM LG 10 × w/o L-Gln, w/o NaHCO3, 500ml
Concentration
w/o Folic Acid
Inorganic Salts mg / L Amino Acids mg / L
1-25K34-I DMEM LG 10 × w/o L-Gln, w/o NaHCO3, 500 ml
CaCl2 2H2O 264 L-Methionine 30
w/o Folic Acid, w/o Phenol Red
Fe(NO3)3 9H2O 0.1 L-Phenylalanine 66
1-25S16-l DMEM w/o L-Gln, w/o Glucose 500ml
MgSO4 7H2O 200 L-Serine 42
1-25P02-K, -L, -M, -N, -W DMEM LG Powder, with L-Gln 1 L, 5 L, 10 L, 50 L, 100 L
KCl 400 L-Threonine 95
1-25P32-K, -L, -M, -N, -W DMEM LG Powder, with L-Gln, 1 L, 5 L, 10 L, 50 L, 100 L
NaCl 6 400 L-Tryptophan 16
w/o Phenol Red
NaH2PO4 H2O 125 L-Tyrosine 72
NaHCO3 3 700 L-Valine 94 Other modifications are available upon request at info@bioconcept.ch.
Other Components Vitamins
D(+)-Glucose 1 000 Choline chloride 4
Phenol Red 15 D-Pantothenic acid calcium salt 4
Pyruvic acid sodium salt 110 Folic acid 4
Amino Acids myo-Inositol 7.2
Glycine 30 Nicotinamide 4
L-Arginine HCl 84 Vitamin B1 HCl 4
L-Cystine 48 Vitamin B2 0.4
L-Histidine HCl H2O 42 Vitamin B6 HCl 4
L-Isoleucine 105 To be added separately
L-Leucine 105 L-Glutamine 584
L-Lysine HCl 146
14 15Dulbecco's MEM High Glucose
References
Cat. No. 1-26F01-I 1. Dulbecco, R. et al.; (1959) Virology 8, 396 9. Dulbecco, R. et al.; (1959) Virology 8, 396
DMEM 2. Smith, J.D. et al.; (1960) Virology 12, 185 10. Smith, J.D. et al.; (1960) Virology 12, 185
High Glucose 3. Morton, H.J. (1970) In Vitro 6, 89 11. Morton, H.J.; (1970) In Vitro 6, 89
4. Rutzky, L.P. et al.; (1974) In Vitro 9, 468 12. Rutzky, L.P. et al.; (1974) In Vitro 9, 468
Concentration 5. Ham, R.G. et al.; (1979) Meth.Enzymol. 53, 44 13. Ham, R.G. et al.; (1979) Meth.Enzymol. 53, 44
Inorganic Salts mg / L Amino Acids mg / L 6. Ham, R.G. (1965) Proc.Nat.Acad.Sci. 53, 288 14. Ham, R.G.; (1965) Proc.Nat.Acad.Sci. 53, 288
CaCl2 2H2O 264 L-Methionine 30 7. Barnes, D. et al.; (1980) Cell 22, 649 15. Barnes, D. et al.; (1980) Cell 22, 649
Fe(NO3)3 9H2O 0.1 L-Phenylalanine 66 8. Barnes, D. et al.; (1980) Anal.Biochem. 102, 255 16. Barnes, D. et al.; (1980) Anal.Biochem. 102, 255
Classical Media
MgSO4 7H2O 200 L-Serine 42
KCl 400 L-Threonine 95
NaCl 6400 L-Tryptophan 16
NaH2PO4 H2O 125 L-Tyrosine 72
NaHCO3 3700 L-Valine 94
Other Components Vitamins
D(+)-Glucose 4500 Choline chloride 4
Phenol Red 15 D-Pantothenic acid calcium salt 4
Amino Acids Folic acid 4
Glycine 30 myo-Inositol 7.2
L-Arginine HCl 84 Nicotinamide 4
L-Cystine 48 Vitamin B1 HCl 4
L-Histidine HCl H2O 42 Vitamin B2 0.4
L-Isoleucine 105 Vitamin B6 HCl 4
L-Leucine 105 To be added separately
L-Lysine HCl 146 L-Glutamine 584
Available Dulbecco's MEM High Glucose Media
Cat. No Modification Size
1-26F01-I DMEM HG w/o L-Gln 500ml
1-26F03-I DMEM HG with L-Gln 500ml
1-26F04-I DMEM HG w/o L-Gln, with 25 mM HEPES 500ml
1-26F22-I DMEM HG w/o L-Gln w/o Phenol Red 500ml
1-26F23-I DMEM HG (4.5 g / L) with stable Gln (868 mg / L), 500ml
w/o Phenol Red
1-26F50-I DMEM HG w/o L-Gln, with stable GIn (868 mg / L) 500ml
1-26F51-I DMEM HG with stable Gln (868 mg / L), 500ml
with 25 mM HEPES
1-26F55-I DMEM HG with 25 mM HEPES, 500ml
with Sodiumpyruvate (110 mg / L)
1-26F58-I DMEM High Glucose with Sodiumpyruvate 500ml
and L-Glutamine
1-26S31-I DMEM HG w/o L-Gln, w/o L-Arg, w/o L-Lys 500ml
1-26P02-K, -L, -M, -N, -W DMEM HG Powder, with L-Gln 1 L, 5 L, 10 L, 50 L, 100 L
1-26P32-K, -L, -M, -N, -W DMEM HG Powder, 1 L, 5 L, 10 L, 50 L, 100 L
with L-Gln, w/o Phenol Red
Other modifications are available upon request at info@bioconcept.ch.
16 17DMEM / Ham's F-12 (1:1) Available DMEM / Ham's F-12 (1:1) Media
Cat. No. 1-26F08-I Cat. No Modification Size
DMEM/F12 1-26F07-I DMEM/F-12 w/o L-Gln, with 15 mM HEPES 500ml
1-26F08-I DMEM/F-12 w/o L-Gln 500ml
Concentration
1-26F09-I DMEM/F-12 with stable GIn (543 mg / L) 500ml
Inorganic Salts mg / L Amino Acids mg / L
1-57F22-I DMEM/F-12 w/o L-Gln, w/o Phenol Red 500ml
CaCl2 2H2O 154 L-Cystine 24
1-57F23-I DMEM/F-12 with stable GIn (543 mg / L), 500ml
CuSO4 5H2O 0.00125 L-Glutamic acid 7.35
w/o Phenol Red
Na2HPO4 anhydrous 71 L-Histidine HCI H2O 31.48
Classical Media
1-57F24-I DMEM/F-12 w/o L-Gln, 500ml
Fe(NO3)3 9H2O 0.05 L-Isoleucine 54.45
with 15 mM HEPES w/o Phenol Red
FeSO4 7H2O 0.417 L-Leucine 59.05
1-57F51-I DMEM/F-12 with 15 mM HEPES, 500ml
MgCl2 6H2O 61 L-Lysine HCI 91.25
with stable GIn (543 mg / L)
MgSO4 7H2O 100 L-Methionine 17.24
1-57F54-I DMEM/F-12 with 15 mM HEPES, 500ml
KCI 311.8 L-Phenylalanine 35.48
with stable GIn (543 mg / L), w/o Phenol Red
NaCl 6999.5 L-Proline 17.25
1-57P02-K, -L, -M, -N, -W DMEM/F-12 Powder with L-GIn 1 L, 5 L, 10 L, 50 L, 100 L
NaH2PO4H2O 62.5 L-Serine 26.25
1-57P06-K, -L, -M, -N, -W DMEM/F-12 Powder with L-GIn, 1 L, 5 L, 10 L, 50 L, 100 L
NaHCO3 2438 L-Threonine 53.45
with 15 mM HEPES, w/o Phenol Red
ZnSO4 7H2O 0.43 L-Tryptophan 9.02
1-57P32-K, -L, -M, -N, -W DMEM/F-12 Powder with L-GIn, 1 L, 5 L, 10 L, 50 L, 100 L
Other Components L-Tyrosine 38.7
w/o Phenol Red
1,4-Diaminobutane 2 HCI 0.08 L-Valine 52.85
D(+)-Glucose 3151 Vitamins Other modifications are available upon request at info@bioconcept.ch.
DL-alpha-Lipoic acid 0.105 (+)-Biotin 0.00365
Hypoxanthine 2.05 Choline chloride 8.98
Linoleic acid 0.042 D-Pantothenic acid calcium salt 2.24
Phenol Red 8.1 Folic acid 2.65
Pyruvic acid sodium salt 55 myo-Inositol 12.6
Thymidine 0.365 Nicotinamide 2.02
Amino Acids Vitamin B1 HCI 2.17
Glycine 18.75 Vitamin B2 0.219
L-Alanine 4.45 Vitamin B6 HCI 2.031
L-Arginine HCI 147.5 Vitamin B12 0.68
L-Asparagine H2O 7.5 To be added separately
L-Aspartic acid 6.65 L-Glutamine 365
L-Cysteine HCI H2O 17.56
18 19Ham's F-10 Medium Available Ham's F-10 Media
Ham's F-10 Medium is a complex formula containing amino acids, vitamins and Cat. No Modification Size
certain supplements and ingredients at optimum concentrations. It was originally 1-13F01-l Ham's F-10 w/o L-GIn 500 ml
developed for serum free culture of Chinese Hamster Ovary (CHO) cells, HeLa cell 1-13F02-l Ham's F-10 with L-GIn 500 ml
clones and mouse “L” cells. Ham's F-10 is today a widely established and popular 1-13F04-l Ham's F-10 w/o L-GIn, with 25 mM HEPES 500 ml
medium for the growth of fastidious cell lines with serum or serum free, eg. diploid 1-13F22-l Ham's F-10 w/o L-GIn, w/o Phenol Red 500 ml
human cells, white blood cells for chromosomal analysis, primary explants of rat, 1-13F50-l Ham's F-10 with stable GIn (217 mg / L) 500 ml
chicken and rabbit.
Other modifications are available upon request at info@bioconcept.ch.
Classical Media
Cat. No. 1-13F01-I
Ham's F-10
References
Concentration 1. Ham, R.G. (1963) Exp. Cell Res. 29, 515-526
2. Ham, R.G. (1965) Proc. Nat. Acad. Sci. 53, 288-293
Inorganic Salts mg / L Amino Acids mg / L
CaCl2 2H2O 44 L-Histidine HCI H2O 23
CuSO4 5H2O 0.0025 L-Isoleucine 2.6
Na2HPO4 anhydrous 154 L-Leucine 13.1
FeSO4 7H2O 0.834 L-Lysine HCI 29
MgSO4 7H2O 153 L-Methionine 4.48
KCI 285 L-Phenylalanine 4.96
KH2PO4 83 L-Proline 11.5
NaCl 7400 L-Serine 10.5
NaHCO3 1200 L-Threonine 3.6
ZnSO4 7H2O 0.0283 Tryptophan 0.6
Other Components L-Tyrosine 1.81
D(+)-Glucose 1100 L-Valine 3.5
DL-alpha-Lipoic acid 0.21 Vitamins
Hypoxanthine 4.1 (+)-Biotin 0.024
Phenol Red 1.2 Choline chloride 0.7
Pyruvic acid sodium salt 110 D-Pantothenic acid Calcium salt 0.715
Thymidine 0.7 Folic acid 1.32
Amino Acids myo-Inositol 0.542
Glycine 7.5 Nicotinamide 0.615
L-Alanine 9 Vitamin B1 HCI 1
L-Arginine HCI 211 Vitamin B2 0.375
L-Asparagine H2O 15 Vitamin B6 HCI 0.205
L-Aspartic acid 13.3 Vitamin B12 1.36
L-Cysteine 25 To be added separately
L-Glutamic acid 14.7 L-Glutamine 146
20 21Ham's F-12 Medium Available Ham's F-12 Media
Ham's F-12 Medium is a modification of the complex Ham's F-10 formula, containing Cat. No Modification Size
amino acids, vitamins and trace elements at optimum concentrations. Originally it 1-14F01-l Ham's F-12 w/o L-GIn 500 ml
was developed for serum free culture of Chinese Hamster Ovary (CHO) cells, primary 1-14F03-l Ham's F-12 with L-GIn 500 ml
Rat Hepatocyytes and Prostate Epithelial Cells. Ham's F-12 is today the medium of 1-14F04-l Ham's F-12 w/o L-GIn, with 25 mM HEPES 500 ml
choice for the clonal toxicity assay with CHO-cells (CHD-3 and CHL-1) and supports 1-14F22-l Ham's F-12 w/o L-GIn, w/o Phenol Red 500 ml
the growth of a variety of normal, fastidious and transformed cells with serum or 1-14F50-l Ham's F-12 with stable GIn (217 mg / L) 500 ml
serum free. 1-14K03-l Ham's F-12 10 ×, w/o L-GIn, w/o NaHCO3 500 ml
Classical Media
1-14K34-l Ham's F-12 10 ×, w/o L-GIn, w/o Phenol Red, 500 ml
Cat. No. 1-14F01-I w/o NaHCO3
Ham's F-12 1-14P02-K, -L, -M, -N, -W Ham's F-12 Powder with L-GIn 1 L, 5 L, 10 L, 50 L, 100 L
1-14S50-l Ham's F-12 Kaighn's Modification 500 ml
Concentration
with stable GIn (434 mg / L)
Inorganic Salts mg / L Amino Acids mg / L
CaCl2 2H2O 44 L-Histidine HCI H2O 20.9 Other modifications are available upon request at info@bioconcept.ch.
CuSO4 5H2O 0.0025 L-Isoleucine 3.9
Na2HPO4 anhydrous 142 L-Leucine 13.1
FeSO4 7H2O 0.834 L-Lysine HCI 36.5 References
MgCl2 6H2O 122 L-Methionine 4.48 1. Ham, R.G.; (1963) Exp.Cell.Res. 29, 515 6. Morton, H.J.; (1970) In Vitro 6, 89
2. Ham, R.G.; (1965) Proc.Nat.Acad.Sci. 53, 288 7. Rutzky, L.P. et al.; (1974) in Vitro 9, 468
KCl 223.6 L-Phenylalanine 4.96
3. Barnes, D. et al.; (1980) Cell. 22, 649 8. Ham, R.G. et al.; (1979) Meth. Enzymol. 53, 44
NaCl 7599 L-Proline 34.5
4. Dulbecco, R. et al.; (1959) Virology, 8 396 9. Barnes, D. et al.; (1980) Anal.Biochem. 102, 255
NaHCO3 1176 L-Serine 10.5 5. Smith, J.D. et al.; (1960) Virology 12, 185 10. Kaighn, M.E.; TCMA (1973) 54-58
ZnSO4 7H2O 0.86 L-Threonine 11.9
Other Components L-Tryptophan 2.04
2'-Deoxythymidine 0.73 L-Tyrosine 5.4
1,4-Diaminobutane 2 HCl 0.16 L-Valine 11.7
D(+)-Glucose 1802 Vitamins
DL-alpha-Lipoic acid 0.21 (+)-Biotin 0.0073
Hypoxanthine 4.1 Choline chloride 13.96
Linoleic acid 0.084 D-Pantothenic acid calcium salt 0.48
Phenol Red 1.2 Folic acid 1.32
Pyruvic acid Sodium salt 110 myo-Inositol 18
Amino Acids Nicotinamide 0.037
Glycine 7.5 Vitamin B1 HCI 0.34
L-Alanine 9 Vitamin B12 1.36
L-Arginine HCl 211 Vitamin B2 0.038
L-Asparagine H2O 15 Vitamin B6 HCI 0.062
L-Aspartic acid 13.3 To be added separately
L-Cysteine HCI H2O 35.12 L-Glutamine 146
L-Glutamic acid 14.7
22 23Iscove's IMDM Available Iscove's IMDM Media
Iscove's IMDM, is the most known and widely used modification of Dulbecco's MEM. Cat. No Modification Size
It is a completely defined, serum free medium, supplemented with albumin, 1-28F01-l IMDM w/o L-GIn, with Supplements 500 ml
transferrin and lecithin, selenium, amino acids, vitamins and HEPES buffer, pyruvate 1-28F02-l IMDM with L-GIn, with Supplements 500 ml
and KNO3 instead of Fe(NO3)3. Iscove's IMDM was originally developed for rapid 1-28F16-l IMDM w/o L-GIn, w/o Supplements 500 ml
propagation of erythropoietic and hemopoietic bone marrow precursor cells in serum 1-28F17-l IMDM with L-GIn, w/o Supplements 500 ml
free media. It is ideal also for hybridoma and T-/B-Lymphocytes cell culture of both, 1-28F22-l IMDM w/o L-GIn, w/o Supplements, 500 ml
human and mouse. w/o Phenol Red
Classical Media
1-28F50-l IMDM w/o Supplements, 500 ml
Cat. No. 1-28F16-I with stable Gln (868 mg / L)
IMDM w/o Supplements 1-28P17-K, -L, -M, -N, -W IMDM Powder, with L-GIn, 1 L, 5 L, 10 L, 50 L, 100 L
w/o Supplements
Concentration
Inorganic Salts mg / L Amino Acids mg / L Other modifications are available upon request at info@bioconcept.ch.
CaCl2 2H2O 219 L-Phenylalanine 66
MgSO4 7H2O 200 L-Proline 40
KCI 330 L-Serine 42 References
KNO3 0.076 L-Threonine 95 1. Iscove, N.N. et. al.; (1978) J.Exp.Med. 147, 923
2. Iscove, N.N. et. al.; (1980) Exp.Cell.Res. 126, 121
NaCl 4505 L-Tryptophan 16
3. Dulbecco, R. et al.; (1959) Virology, 8, 396
NaH2PO4 2H2O 141 L-Tyrosine 72
4. H
arbour, C. et. al.; (1991) in Mammalian Cell
NaHCO3 3024 L-Valine 94 Biotechnology, 1st Ed.; IRL Press, Oxford – NY – Tokyo
NaSeO3 5H2O 0.017 Vitamins
Other Components (+)-Biotin 0.013
D(+)-Glucose 4500 Choline chloride 4
HEPES 5958 D-Pantothenic acid calcium salt 4
Phenol Red 15 Folic acid 4
Pyruvic acid sodium salt 110 myo-Inositol 7.2
Amino Acids Nicotinamide 4
Glycine 30 Vitamin B1 HCl 4
L-Alanine 25 Vitamin B2 0.4
L-Arginine HCl 84 Vitamin B6 HCl 4
L-Asparagine H2O 28.4 Vitamin B12 0.013
L-Aspartic acid 30 To be added separately
L-Cystine 70 L-Glutamine 584
L-Glutamic acid 75 Iscove's Supplemented Media contain
L-Histidine HCI H2O 42 BSA 400
L-Isoleucine 105 Linoleic acid 1
L-Leucine 105 Oleic acid 1
L-Lysine HCI 146 Palmitic acid 1
L-Methionine 30 Transferrin 1
24 25Leibovitz L-15 Medium
Medium 199
Leibovitz L-15 Medium is a complex NaHCO3-free formulation for use in carbon
Medium 199 is a totally defined nutritive source for explant tissues and cells in cell
dioxide free cell culture systems. It is buffered by its complement salts, free base
culture. Properly supplemented with serum, Medium 199 offers broad applicability for
amino acids, galactose and phosphates to maintain physiological pH conditions.
cells of different animal species, particularly for the culture of non-transformed cells.
Leibovitz L-15 Medium supports the growth and proliferation of a variety of
It is also used in virology for vaccine production.
established cell lines, eg. HEp-2, as well as primary explants of embryonic and adult
human tissues, eg. nerve tissue. It is supplemented with 10 % Tryptose Phosphate
Cat. No. 1-21F01-I
Broth, L-15 is highly suitable for the culture of insect cells, e.g. bed mug, mosquitoes M 199 EBS
Classical Media
and ticks.
Concentration
Cat. No. 1-17F01-I Inorganic Salts mg / L Amino Acids mg / L
Leibovitz L-15 CaCl2 2H2O 265 L-Glutamic acid 66.82
Fe(NO3)3 9H2O 0.72 L-Histidine HCL, H2O 21.88
Concentration
MgSO4 7H2O 200 L-Isoleucine 20
Inorganic Salts mg / L Amino Acids mg / L
KCI 400 L-Leucine 60
CaCl2 2H2O 185,4 L-Asparagine anhydrous 250
NaCl 6 800 L-Lysine HCl 70
Na2HPO4 anhydrous 190 L-Cyteine 120
NaH2PO4 H2O 140 L-Methionine 15
MgCl2 6H2O 200 L-Histidine 250
NaHCO3 2 200 L-Phenylalanine 25
MgSO4 7H2O 200 L-Leucine 125
Other Components L-Proline 40
KCI 400 L-Lysine 75
2-Deoxy-D-ribose 0.5 L-Serine 25
KH2PO4 60 L-Serine 200
4-Aminobenzoic acid 0.05 L-Threonine 30
NaCl 8 000 L-Tryptophan 20
Adenine sulfate 10 L-Tryptophan 10
Other Components L-Tyrosine 300
AMP H2O 0.25 L-Tyrosine 40
D(+)-Galactose 900 Vitamins
ATP disodium salt H2O 1 L-Valine 25
Phenol Red 10 Choline chloride 1
Cholesterol 0.2 Vitamins
Pyruvic acid sodium salt 550 D-Pantothenic acid calcium salt 1
D(+)-Glucose 1 000 (+)-Biotin 0.01
Amino Acids Folic acid 1
D(-)-Ribose 0.5 Vitamin K3 0.02
L-Alanine 225 myo-Inositol 2
Guanine HCI 0.3 Choline chloride 0.5
L-Isoleucine 125 Nicotinamide 1
Hypoxanthine 0.3 D-Pantothenic acid calcium salt 0.01
L-Methionine 75 Riboflavin 5-Phosphate Na 0.1
L-Glutathione reduced 0.05 Folic acid 0.01
L-Phenylalanine 125 Thiamin monophosphate 1
Phenol Red 20 myo-Inositol 0.05
L-Threonine 300 Vitamin B6 HCl 1
CH3COONa anhydrous 50 Nicotinamide 0.025
L-Valine 100 To be added separately
Thymine 0.3 Nicotinic acid 0.025
Glycine 200 L-Glutamine 300
Tween 80 20 Pyridoxal HCl 0.025
L-Arginine 500
Uracil 0.3 Vitamin B1 HCl 0.01
Other modifications are available upon request at info@bioconcept.ch. Xanthine 0.3 Vitamin B2 0.01
Amino Acids Vitamin B6 HCl 0.025
Glycine 50 Vitamin C 0.05
References L-4-Hydroxyproline 10 Vitamin D2 0.1
1. Leibovitz, A.; (1963) Amer.J.Hyg. 78, 173 L-Alanine 25 Vitamin E succinate 0.01
2. Yunker, C.E. et al.; (1979) TCA Manual 5, 1077
L-Arginine HCl 70 Vitamin A acetate 0.14
L-Aspartic acid 30 To be added separately
L-Cysteine HCL, H2O 0.11 L-Glutamine 100
L-Cystine 20
26 27Cat. No. 1-22F01-I Available Medium 199 Media
M 199 HBS (Liquid)
Cat. No Modification Size
Concentration 1-21F01-l M 199 EBS w/o L-GIn 500 ml
Amino Acids mg / L Vitamins mg / L 1-21F04-l M 199 EBS w/o L-GIn, with 25 mM HEPES 500 ml
L-Alanine25 p-Aminobenzoic Acid 0.05 1-21F22-l M 199 EBS w/o L-GIn w/o Phenol Red 500 ml
L-Arginine, HCI 70 Pyridoxin, HCI 0.025 1-21F50-l M 199 EBS with stable GIn (149 mg / L) 500 ml
L-Aspartic Acid 30 Pyridoxal, HCI 0.025 1-22F01-l M 199 HBS w/o L-GIn 500 ml
L-Cysteine, HCI H2O0.11 Riboflavin0.01 1-22F50-l M 199 HBS with stable GIn (149 mg / L) 500 ml
Classical Media
L-Cystine20 Thiamine, HCI 0.01
Other modifications are available upon request at info@bioconcept.ch.
L-Glutamic Acid 66.82 Vitamin A-acetate 0.14
Glycin50 Inorganic Salts
L-Histidine, HCI H2O21.88 CaCl2 2H2O185.5
References
L-4-Hydroxyproline10 NaCl 8 000 1. Morgan, J.F. et al.; (1950) Proc.Soc.Exp.Biol.Med. 73, 1
L-Isoleucine20 KCl400 2. Morgan, J.F. et al.; (1951) Nat. Canc.Inst. 16/2, 557
L-Leucine60 Fe(NO3)3 9H2O0.72
L-Lysine, HCI 70 KH2PO460
L-Methionine15 MgSO4 7H2O200
L-Phenylalanine25 NaH2PO4 anhydrous 47.5
L-Proline40 NaHCO3350
L-Serine25 Others mg / L
L-Threonine30 Adenosine 5'-triphosphate 2Na H2O1
L-Tryptophan10 Adenosine 5'-phosphate H2O0.25
L-Tyrosin40 Adenine Sulphate 10
L-Valine25 L-Glutathione (red.) 0.05
Vitamins Guanine HCI 0.3
Ascorbic Acid 0.05 Deoxy-D-ribose0.5
Tocopherolsuccinate DL-alfa 0.01 D-Ribose0.5
D-Biotin0.01 Glucose, D (+) 1 000
Calciferol0.1 Hypoxanthine0.3
D-Pantothenic Acid (Ca-Salt) 0.01 Sodium Acetate (anhydr.) 50
Choline Chloride 0.5 Tween 80 20
Folic Acid 0.01 Xanthine0.3
Inositol0.05 Uracil0.3
Menadione0.02 Thymine0.3
Niacin0.025 Phenol red 20
Niacinamide0.025 Cholesterol0.2
28 29McCoy's 5A
Available McCoy's 5A Media
McCoy's 5A Medium was created using Basal Medium 5A and is a nutritive
Cat. No Modification Size
substance rich complete medium. McCoy's 5A Medium was originally developed
1-18F01-l McCoy's 5A w/o L-GIn 500 ml
to investigate the amino acid requirements of Novikoff Hepatoma cells. Today this
1-18F03-l McCoy's 5A with L-GIn 500 ml
optimal developed medium has a broad application to support the growth of the
1-18F22-l McCoy's 5A w/o L-GIn (326 mg / L), 500 ml
most demanding primary and continuous cell lines, as well as from biopsy tissues,
w/o Phenol Red
eg. bone marrow, skin, kidney, omentum, adrenal glands, lung, spleen.
1-18F23-l McCoy's 5A with stable GIn (326 mg / L), 500 ml
w/o Phenol Red
Classical Media
Cat. No. 1-18F01-I
McCoy's 5A 1-18F50-l McCoy's 5A with stable GIn (326 mg / L) 500 ml
1-18F51-l McCoy's 5A with 25 mM HEPES, 500 ml
Concentration with stable GIn (326 mg / L)
Inorganic Salts mg / L Amino Acids mg / L 1-18P02-K, -L, -M, -N, -W McCoy's 5A Powder, with L-GIn 1 L, 5 L, 10 L, 50 L, 100 L
CaCl2 2H2O 132.5 L-Methionine 14.9
Other modifications are available upon request at info@bioconcept.ch.
MgSO4 7H2O 200 L-Phenylalanine 16.5
KCl 400 L-Proline 17.3
NaCl 6 460 L-Serine 26.3
References
NaH2PO4 H2O 580 L-Threonine 17.9 1. Neumann, R.E. et al.; (1958) Proc.Soc.Exp.Biol.Med. 98, 303
NaHCO3 2 200 L-Tryptophan 3.1 2. McCoy, T.A. et al.; (1959) Proc.Soc.Exp.Biol.Med. 100, 115
Other Components L-Tyrosine 18.1 3. Hsu, T.C. et al.; (1960) J.Natl.Canc.Inst. 25, 221
4-Aminobenzoic acid 1 L-Valine 17.6 4. Iwakata, S. et al.; (1964) N.Y. State J.Med. 64, 2279
Bacto Peptone 600 Vitamins 5. Morton, H.J.; (1970) In Vitro 6, 89
6. Park, M.S. et al.; (1974) Transpl. 18, 520
D(+)-Glucose 3 000 (+)-Biotin 0.2
7. Patterson, M.K. et al.; (1978) TCA Manual 4, 737
L-Glutathione reduced 0.5 Choline chloride 5
Phenol Red 10 D-Pantothenic acid calcium salt 0.2
Amino Acids Folic acid 10
Glycine 7.5 myo-Inositol 36
L-4-Hydroxyproline 19.7 Nicotinamide 0.5
L-Alanine 13.9 Nicotinic acid 0.5
L-Arginine HCl 42.1 Pyriodoxal HCl 0.5
L-Asparagine anhydrous 45 Vitamin B1 HCl 0.2
L-Aspartic acid 19.97 Vitamin B2 0.2
L-Cysteine 31.5 Vitamin B6 HCl 0.5
L-Glutamic acid 22.1 Vitamin B12 2
L-Histidine HCI H2O 20.96 Vitamin C 0.5
L-Isoleucine 39.36 To be added separately
L-Leucine 39.36 L-Glutamine 219.2
L-Lysine HCl 36.5
30 31MEM Alpha Available MEM Alpha Media
MEM Alpha is a highly complex formulation and is enriched with additional amino Cat. No Modification Size
acids, vitamins, lipoic acid and pyruvate. It is highly enriched if supplemented 1-23F01-l MEM Alpha w/o L-GIn, with Nucleosides 500 ml
with ribo- and desoxyribonucleosides. Originally used for growth of hamster kidney 1-23F09-l MEM Alpha w/o L-GIn, w/o Nucleosides 500 ml
cells, MEM Alpha supports the growth and proliferation of bone marrow and 1-23F22-l MEM Alpha w/o L-GIn, w/o Nucleosides, 500 ml
amniotic cells, both in monolayer- and suspension-culture. Supplementation with w/o Phenol Red
serum or certain proteins supports the culture. BioConcept Ltd. offers MEM Alpha 1-23F23-l MEM Alpha w/o L-GIn, with Nucleosides, 500 ml
with and without nucleosides as liquid / powder variations. Modifications are w/o Phenol Red
Classical Media
possible on customer requests. 1-23S50-l MEM Alpha with stable GIn (434 mg / L), 500 ml
with Nucleosides
Cat. No. 1-23F01-I 1-23F50-l MEM Alpha with stable GIn (434 mg / L), 500 ml
MEM Alpha with Nucleosides w/o Nucleosides
1-23P10-K, -L, -M, -N, -W MEM Alpha w/o Nucleosides, Powder 1 L, 5 L, 10 L, 50 L, 100 L
Concentration
Inorganic Salts mg / L Amino Acids mg / L Other modifications are available upon request at info@bioconcept.ch.
CaCl2 2H2O 265 L-Threonine 48
MgSO4 7H2O 200 L-Tryptophan 10
KCl 400 L-Tyrosine 36 References
NaCl 6 800 L-Valine 46 1. Stanners, C.P. et al.; (1971) Nat.New.Biol. 230, 52
2. Stanners, C.P. et al.; (1975) J.Gen.Virol. 29, 281
NaH2PO4 H2O 140 Vitamins
3. Earle, W. (1943) J.Natl.Cancer.Inst. 4, 165
NaHCO3 2 200 (+)-Biotin 0.1
Other Components Choline chloride 1
D(+)-Glucose 1 000 D-Pantothenic acid calcium salt 1
DL-alpha-lipoic acid 0.2 Folic acid 1
Phenol Red 10 myo-Inositol 2
Pyruvic acid sodium salt 110 Nicotinamide 1
Amino Acids Pyriodoxal HCl 1
Glycine 50 Vitamin B1 HCl 1
L-Alanine 25 Vitamin B2 0.1
L-Arginine HCl 127 Vitamin B12 1.4
L-Asparagine H2O 50 Vitamin C 50
L-Aspartic acid 30 Ribonucleosides
L-Cysteine 100 Adenosine 10
L-Cystine 24 Cytidine 10
L-Glutamic acid 75 Guanosine 10
L-Histidine HCI H2O 42 Uridine 10
L-Isoleucine 52 Deoxyribonucleosides
L-Leucine 52 2'-Deoxyadenosine 10
L-Lysine HCI 73 2'-Deoxycytidine HCl 11
L-Methionine 15 2'-Deoxyguanosine H2O 10.67
L-Phenylalanine 32 2'-Deoxythymidine 10
L-Proline 40 To be added separately
L-Serine 25 L-Glutamine 292
32 33MEM EBS and MEM HBS Available MEM EBS Media
MEM Eagle, a complex amino acid and vitamin rich medium, was developed from Cat. No Modification Size
Eagle's Basal Medium (BME). Based on Eagle's Hank's BSS, MEM is one of the most 1-31F01-l MEM EBS w/o L-GIn 500 ml
widely used of all synthetic cell culture media. MEM Eagle with EBS or HBS supports 1-31F03-l MEM EBS with L-GIn 500 ml
the growth and proliferation of a variety of fastidious primary mammalian cells and 1-31F04-l MEM EBS w/o L-GIn, with 25 mM HEPES 500 ml
established cell lines, both in monolayer- and suspension-culture. Supplementation 1-31F18-l MEM EBS w/o L-GIn, w/o Leucine 500 ml
with serum or certain proteins supports the culture. 1-31F19-l MEM EBS w/o L-GIn, w/o Phosphate 500 ml
1-31F20-l MEM EBS w/o L-GIn, w/o Methionine 500 ml
Classical Media
Cat. No. 1-31F01-I 1-31F21-l MEM EBS w/o L-GIn, with 25 mM HEPES, 500 ml
MEM EBS w/o Folic acid
1-31F22-l MEM EBS w/o L-GIn, w/o Arginine 500 ml
Concentration
1-31F24-l MEM EBS w/o L-GIn, w/o Phenol Red 500 ml
Inorganic Salts mg / L Amino Acids mg / L
1-31F50-l MEM EBS with stable GIn (434 mg / L) 500 ml
CaCl2 2H2O 267 L-Phenylalanine 33
1-31F51-l MEM EBS with 25 mM HEPES, 500 ml
MgSO4 7H2O 200 L-Threonine 47.6
with stable GIn (434 mg / L)
KCl 400 L-Tryptophan 10.2
1-31P02-K, -L, -M, -N, -W MEM EBS Powder with L-GIn 1 L, 5 L, 10 L, 50 L, 100 L
NaCl 6 800 L-Tyrosine 36.2
1-31P05-K, -L, -M, -N, -W MEM EBS Powder, with L-GIn, 1 L, 5 L, 10 L, 50 L, 100 L
NaH2PO4 H2O 140 L-Valine 46
with 25 mM HEPES
NaHCO3 2 200 Vitamins
Other Components Choline chloride 1 Other modifications are available upon request at info@bioconcept.ch.
D(+)-Glucose 1 000 D-Pantothenic acid calcium salt 1
Phenol Red 10 Folic acid 1
Amino Acids myo-Inositol 2 References
L-Arginine HCl 126.4 Nicotinamide 1 1. Eagle, H. et al.; (1956) J.Biol.Chem. 214, 845
2. Eagle, H. (1955) Science 122, 501
L-Cystine 24 Pyridoxal HCl 1
3. Eagle, H. (1959) Science 130, 432
L-Histidine HCI H2O 41.93 Vitamin B1 HCl 1
4. Parker, R.C. (1961) In: Meth.Tis.Cult.; 3rd Ed. Harper NY
L-Isoleucine 52 Vitamin B2 0.1 5. Eagle, H. (1976) TCA Manual 3, 517
L-Leucine 52 To be added separately 6. Daniel, M.D. et al.; (1969) Proc.Soc.Exp.Biol.Med. 127, 3
L-Lysine HCl 73.06 L-Glutamine 292 7. Ham, R.G. et al.; (1979) Meth.Enzymol. 53, 44
L-Methionine 14.9 8. Earle, W. (1943) Natl.Cancer.Inst. 4, 165
34 35MEM HBS RPMI 1640
Cat. No. 1-33F01-I RPMI 1640 was developed as a modification of McCoy's 5A medium and is based
MEM HBS on RPMI 1630. RPMI 1640 uses a bi-carbonate buffering system and differs from
most mammalian cell culture media in its typical pH 8 formulation. With or without
Concentration
supplemented serum or other defined proteins, RPMI 1640 represents today’s most
Inorganic Salts mg / L Amino Acids mg / L
widely applied culture medium for short as well as long term cultivation of many
CaCl2 2H2O 185.4 L-Methionine 14.9
cell types, especially human T / B-lymphocytes (e.g. 72 h PHA stimulation assay), bone
Na2HPO4 anhydrous 47.5 L-Phenylalanine 33
marrow cells, hybridoma cells.
MgSO4 7H2O 200 L-Threonine 47.6
Classical Media
KCl 400 L-Tryptophan 10.2
Cat. No. 1-41F01-I
KH2PO4 60 L-Tyrosine 36.2 RPMI 1640
NaCl 8 000 L-Valine 46
NaHCO3 350 Vitamins Concentration
Other Components Choline chloride 1 Inorganic Salts mg / L Amino Acids mg / L
D(+)-Glucose 1 000 D-Pantothenic acid calcium salt 1 Ca(NO3)2 4H2O 100 L-Lysine HCl 40
Phenol Red 10 Folic acid 1 Na2HPO4 anhydrous 800 L-Methionine 15
Amino Acids myo-Inositol 2 MgSO4 7H2O 100 L-Phenylalanine 15
L-Arginine HCl 126.4 Nicotinamide 1 KCl 400 L-Proline 20
L-Cystine 24 Pyridoxal HCl 1 NaCl 6 000 L-Serine 30
L-Histidine HCI H2O 41.93 Vitamin B1 HCl 1 NaHCO3 2 000 L-Threonine 20
L-Isoleucine 52 Vitamin B2 0.1 Other Components L-Tryptophan 5
L-Leucine 52 To be added separately 4-Aminobenzoic acid 1 L-Tyrosine 20
L-Lysine HCl 73.06 L-Glutamine 292 D(+)-Glucose 2 000 L-Valine 20
L-Glutathione reduced 1 Vitamins
Phenol Red 5 (+)-Biotin 0.2
Available MEM HBS Media
Amino Acids Choline chloride 3
Cat. No Modification Size Glycine 10 D-Pantothenic acid calcium salt 0.25
1-33F01-l MEM HBS w/o L-GIn 500 ml L-4-Hydroxyproline 20 Folic acid 1
1-33F50-l MEM HBS with stable GIn (434 mg / L) 500 ml L-Arginine HCl 241.9 myo-Inositol 35
1-33F51-l MEM HBS with 25 mM HEPES, 500 ml L-Asparagine anhydrous 50 Nicotinamide 1
with stable GIn (434 mg / L) L-Aspartic acid 20 Vitamin B1 HCl 1
L-Cystine 50 Vitamin B2 0.2
Other modifications are available upon request at info@bioconcept.ch.
L-Glutamic acid 20 Vitamin B6 HCl 1
L-Histidine 15 Vitamin B12 0.005
L-Isoleucine 50 To be added separately
References
1. Hanks, J.H.; (1976) TCA Manual 3, 3 L-Leucine 50 L-Glutamine 300
2. Hanks, J.H. et al.; (1949) Proc.Soc.Exp.Biol.Med. 71, 19
36 37Available RPMI 1640 Media Waymouth's Medium
Cat. No Modification Size Waymouth's Medium was developed as a serum free, chemically defined synthetic
1-41F01-l RPMI 1640 w/o L-GIn 500 ml medium for the culture of mouse cell line L929.
1-41F03-l RPMI 1640 with L-GIn 500 ml
1-41F04-l RPMI 1640 w/o L-GIn with 25 mM HEPES 500 ml Cat. No. 1-46F01-I
1-41F22-l RPMI 1640 w/o L-GIn, w/o Phenol Red 500 ml Waymouth's MB752/1
1-41F23-l RPMI 1640 with stable GIn (446 mg / L), 500 ml
Concentration
w/o Phenol Red
Inorganic Salts mg / L Amino Acids mg / L
Classical Media
1-41F24-l RPMI 1640 w/o L-GIn, with 25 mM HEPES, 500 ml
CaCl2 2H2O 120 L-Lysine HCl 240
w/o Phenol Red
MgCl2 6H2O 240 L-Methionine 50
1-41F50-l RPMI 1640 with stable GIn (446 mg / L) 500 ml
MgSO4 7H2O 200 L-Phenylalanine 50
1-41F51-l RPMI 1640 with 25 mM HEPES, 500 ml
KCl 150 L-Proline 50
with stable GIn (446 mg / L)
NaCl 6 000 L-Threonine 75
1-41F54-l RPMI 1640, with 25 mM HEPES, 500 ml
Na2HPO4 anhydrous 300 L-Tryptophan 40
with stable GIn, w/o Phenol Red
KH2PO4 80 L-Tyrosine 40
1-41S07-l RPMI 1640, w/o L-GIn, w/o Glucose 500 ml
NaHCO3 2 240 L-Valine 65
1-41S37-l RPMI 1640 w/o L-GIn, w/o L-Arg, w/o L-Lys 500 ml
Other Components Vitamins
1-41K03-l RPMI 1640 10 × w/o L-GIn, w/o NaHCO3 500 ml
D(+)-Glucose 5 000 (+)-Biotin 0.02
1-41K34-l RPMI 1640 10 × w/o L-GIn, 500 ml
Glutathione, reduced 15 Choline chloride 250
w/o NaHCO3, w/o Phenol Red
Hypoxanthine 25 D-(Pantothenic acid calcium salt 1
1-41P02-K, -L, -M, -N, -W RPMI 1640 Powder with L-GIn 1 L, 5 L, 10 L, 50 L, 100 L
Phenol Red 10 Folic acid 0.5
1-41P05-K, -L, -M, -N, -W RPMI 1640 Powder with L-GIn, 1 L, 5 L, 10 L, 50 L, 100 L
Amino Acids myo-Inositol 1
with 25 mM HEPES
Glycine 50 Nicotinamide 1
1-41P32-K, -L, -M, -N, -W RPMI 1640 Powder with L-GIn, 1 L, 5 L, 10 L, 50 L, 100 L
L-Arginine HCl 75 Pyridoxine HCl 1
w/o Phenol Red
L-Aspartic acid 60 Vitamin B1 HCl 10
Other modifications are available upon request at info@bioconcept.ch. L-Cysteine HCI H2O 100.3 Vitamin B2 1
L-Cystine 15 Vitamin B12 0.20
L-Glutamic acid 150 Vitamin C 17.5
References L-Histidine HCI H2O 164.1 To be added separately
1. Moore, G.E. et al.; (1967) JAMA 199, 519 L-Isoleucine 25 L-Glutamine 350
2. Moore, G.E. et al.; (1976) TCA Manual, 3, 503
L-Leucine 50
3. Moore, G.E. et al.; (1967) XXl.Ann.Symp.Fund.Canc.Res.Feb., 41
4. Moore, G.E. et al.; (1968) NY.State J.Med. 68, 2054
5. Ham, R.G. et al.; (1979) Meth.Enzymol. 53, 44 Available Waymouth's Media
Cat. No Modification Size
1-46F01-l Waymouth's MB 752/1 w/o L-GIn 500 ml
1-46F22-l Waymouth's MB 752/1 w/o L-GIn, w/o Phenol Red 500 ml
Other modifications are available upon request at info@bioconcept.ch.
References
1. Waymouth, C.; (1959) J.Nat.Cancer Inst. 22, 1003
2. Waymouth, C.; (1968) Private Communication
38 39William's E Medium Available William's E Media
William's E Medium was developed for long term culture of liver epithelial cells of Cat. No Modification Size
adult rats. 1-48F01-l William's E Medium w/o L-GIn 500 ml
1-48F02-l William's E Medium w/o L-GIn, w/o Phenol Red 500 ml
Cat. No. 1-48F01-I
William's E Medium Other modifications are available upon request at info@bioconcept.ch.
Concentration
Inorganic Salts mg / L Amino Acids mg / L References
Classical Media
CaCl2 2H2O 265 L-Lysine HCl 87.6 1. Williams, G.M. et al.; 1974; Exp.Cell Res. 89, 139
2. Williams, G.M. et al.; 1971; Exp.Cell Res. 69, 106
CuSO4 5H2O 0.0001 L-Methionine 15
Fe(NO3)3 9H2O 0.0001 L-Phenylalanine 25
MgSO4 7H2O 200 L-Proline 30
MnCl4 4H2O 0.0001 L-Serine 10
KCl 400 L-Threonine 40
NaCl 6 800 L-Tryptophan 10
NaH2PO4 2H2O 158 L-Tyrosine 35
NaHCO3 2 200 L-Valine 50
ZnSO4 7H2O 0.0002 Vitamins
Other Components (+)-Biotin 0.5
D(+)-Glucose 2 000 Vitamin K3 0.01
L-Glutathione reduced 0.05 Choline chloride 1.5
Methyl linolenate 0.03 D-Pantothenic acid calcium salt 1
Phenol Red 10 Folic acid 1
Pyruvic acid sodium salt 25 myo-Inostol 2
Amino Acids Nicotinamide 1
Glycine 50 Pyridoxal HCl 1
L-Alanine 90 Vitamin B1 HCl 1
L-Arginine 50 Vitamin B2 0.1
L-Asparagine H2O 20 Vitamin B12 0.2
L-Aspartic acid 30 Vitamin C 2
L-Cysteine 40 Vitamin D2 0.1
L-Cystine 20 Vitamin E succinate 0.01
L-Glutamic acid 50 Vitamin A acetate 0.1
L-Histidine 15 To be added separately
L-Isoleucine 50 L-Glutamine 292
L-Leucine 75
40 41Special
Media
MAM-PF (Production Media)
Hybridoma Express Media
Insect Cell Culture Media
BSK-H Medium for the cultivation
of Borrelia spec.
SDM 79 for the cultivation
of Trypanosoma spec.
Special Media
Stem Cell Media
Sea anemone [ Actiniaria ]
Sea anemone is the umbrella term used for this
predatory, colourful animal. They mainly live in
the world’s corals and are quite related to corals
and jellyfish, however, unlike jellyfish, a typical
sea anemone is attached to a hard surface and
lacks the free swimming stage of its life cycle.
They are known to make bonds with other
species in their environments to improve their
chance of survival, the most famous one being
with the clownfish. The clownfish protects
the sea anemone from predators, and in return
the sea anemone offers the fish a place to
call home.
42 43Special Media
MAM-PF® for Serum-Free, Protein-Free and Animal Component
Free (ACF) Cell Culture
The breakthroughs in recombinant protein production granted BioConcept Ltd. the
ability to produce and supply a new type of cell culture media. BioConcept Ltd.’s
MAM-PF® (Mammalian Artificial Media – Protein Free) line of media are tailor made to
satisfy the needs of researchers and producers. These media for the cultivation
of CHO and BHK cells are fully defined with serum-free, protein-free and ACF (Animal
Component Free) versions. ACF media are produced according to EMEA/410/01
all components are certified animal component free – we hold a certificate of origin
for every single component.
Special Media
Products Available
Cat. No. Description Size Serum Protein Animal Formulation
free free Component
free
(ACF)
Mammalian artificial Media – Animal Component free
10-02F24-I MAM-PF® 2 growth 500 ml × × × Proprietary
and production medium
w/o Phenol Red
10-02F25-I MAM-PF® 2 growth 500 ml × × × Proprietary
and production medium
with Phenol Red
10-02F27-I MAM-PF® 1 adaptation 500 ml × × × Proprietary
and growth medium
10-02F33-I MAM-PF® 2 Total 500 ml × × × Proprietary
Selection Medium
(w/o L-Gln, L-Trp, L-His,
Gly, Hypoxanthine
and Thymidine)
10-02S80-I MAM-PF®77 500 ml × × × Proprietary
production medium
10-02P74 MAM-PF® 7e flexible × × × Proprietary
Powder Media
10-02P24 MAM-PF® 2 1 L, 5 L, 10 L, × × × Proprietary
-K,-L,-M,-N,-W growth and production 50 L, 100 L
medium (powder)
w/o Phenol Red,
w/o L-Gln, w/o NaHCO3
5-77F01-H Lipid-Mixture for MAM-PF® 100 ml × × × Proprietary
2 Powder (1 000 ×)
44 45Performance
Cell Density MAM-PF® and Other Suppliers
MAM-PF® Media
CHOSI cells cultured in 35
100
MAM-PF77® have shown
Mammalian Artificial Media – Protein and Animal Component Free (ACF) for CHO, the fastest growth and a
30
Living Cells [%]
25 80
~100% and ~40% higher
BHK, and other mammalian cells MAM-PF® and other Suppliers
Living cells [10E9/L]
cell density compared to
20
Further development of CHO-Express Media resulted in our MAM-PF® Media Series. 60 Performance of MAM-PF77®
Application Data Antibody
media by Suppliers S and
T. This corresponds with 15 and two different CHO media
These are chemically defined media for the cultivation of a variety of cell lines such the final product titers at 40
Titer [%]
the end of the fed-batch, 10 suppliers in a 14-day fed-batch
as CHO (Chinese Hamster Ovary) Cells or BHK cells and expression of recombinant respectively (All cultures 20
5 mAb production.
proteins in suspension cell culture. CHO and BHK cells are the most widely used were fed with FMS3 in
the same feed regime). 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
mammalian cells for the expression of a variety of recombinant proteins. The higher viability in the Culture days
stationary phase shows that
MAM-PF® Media are formulated without L-glutamine to avoid problems associated the glycosylation in MAM- BioConcept Supplier S Supplier T
PF77® was superior.
with L-glutamine degradation including ammonia accumulation. MAM-PF® Media can Living cells [10E9/L] Titer [%]
be obtained with a minimal amount of Phenol Red or without Phenol Red. Performance of MAM-PF77® and two different CHO media suppliers in a 14-day fed-batch mAb
production.
Serum-free culture represents an advance over serum supplemented culture as this FSH
FSH produced with MAM-PF® and FMS3
facilitates purification and downstream processing of expressed products. Most A cell density of over 100
1000 mio. cells per liter can be
of the commercially available serum-free media contain various undefined proteins reached through mixing
Cell Density [E8/L], Viability [%], Product [mg/L]
12
the CHO feed mix FMS3
and/or protein hydrolysates. A completely defined formulation is therefore a major to MAM-PF77®. It is
FSH produced with 10
Special Media
advantage over both serum supplemented and serum-free systems. The absence of 100 possible to achieve a titer
Glucose/lLactate [g/L], pH
MAM-PF® and FMS3 8 of over 350 mg/L of the
animal derived proteins is desirable from a regulatory standpoint and also eliminates 14-day bioreactor production
highly glycosylated follicle-
6 stimulating hormone (FSH)
concerns about consistency and availability. MAM-PF®1, MAM-PF®2, MAM-PF®7d scheme of the high glycosylated 10
within 14 days in a stirring
4 bioreactor tank , making it
and MAM-PF®7e media are chemically defined media optimized for the growth of follicle-stimulating hormone (FSH) a very high quality product.
2 As determined during the
Chinese hamster ovary (CHO) or BHK cells and expression of recombinant proteins using MAM-PF77® and the FMS3
MAM-PF
purification process, 45%
feed mix. ®
of the product showed
in suspension culture. MAM-PF® media contain no proteins or peptides of animal 24 48 72 96 120 144 168 192 216 240 264 288 312 336
an isoform-pattern, low
Runtime [h]
origin and no undefined hydrolysates. L-glutamine (0.6 – 8.00 mM) has to be added by Total Cells Living Cells Viability FSH Glucose pH Lactate
aggregates, and low
oxidized forms. MAM-PF77®
the end user prior to use. Various customers reported best glycosylation patterns can be used to produce
14-day bioreactor production scheme of the high glycosylated follicle-stimulating hormone (FSH) using quality FSH that fulfills its
of the recombinant expressed product when using MAM-PF® Media. MAM-PF77® and the FMS3 feed mix. Ph.Eur. requirements.
Highlights of the MAM-PF® series
Ipilimumab Ipilimumab produced with MAM-PF® and FSU
5
The continuous innovation 25 Animal Component Free 6.0 Liquid batch capacity
®
and development of the MAM-PF media do not contain proteins or undefined hydroly- Batch sizes ranging from 5-5000 litre, water for injection (WFI) is the
sates. 5.0 highest quality available.
MAM-PF® media series 20 Ipilimumab produced with
has lead to the brand new Chemically defined 4.0
MAM-PF Powder
®
and batch FSUcapacity
Living Cells 10E9/L
MAM-PF77® cell culture 15
Titer (g/L)
BioConcept holds TSE certificates for each component to ensure Batch sizes ranging from 1 - 800 kg, our milling process results in
medium and CHO Feed EMA/410/01 conformity. 3.0 High yields of mAbs,
particle sizes of arounde.g. > 5This
20 µm (d50). g/Lleads to a quick dissoluti-
on of the powder medium.
Mixes FMS3 and FMU. 10
MAM-PF® media now
Easy adaptation 2.0 Iplimumab can be reached in a
In many cases it is possible to switch directly from your current Feed mixes
increase productivity of 5 medium to MAM-PF . ®
1.0
14-day fed-batch
Various feed mixessystem
are availableusing
for high density cell culture and
4 Ipilimumab (monoclonal high
® productivity.
antibody) by up to 5g/L. High cell density combined with high product MAMPF77
yield plus the novel CHO
Glycosylation
The new expression system 0 1 2 3 4 5 6 7 8 9 10- Antibody
11 production
12 of up to 5.5 g/L.
13 14
- EPO of up to 2.5 g/L (see graph below).
feed mix FMU.
Best glycosylation pattern observed.
is also viable in fed-batch Culture days - Cell density up to 3.7 x 107 cells/ml.
and perfusion systems. Titer Viable cells
High yields of mAbs, e.g. > 5g/L Iplimumab can be reached in a 14-day fed-batch system using MAM-
PF77® plus the novel CHO feed mix FMU.
MAM-PF® series
Development of MAM-PF® MAM-PF® (Mammalian
Artificial Medium - Protein
2500
6 Free) media are Animal
Component Free (ACF)
2000
and in accordance with
Development of MAM-PF® the strict quality guidelines
EMA/410/01. MAM-PF®
Increase of Erythropoietin (EPO) is a production media.
[mg/L]
1500
It is protein-free and
yields during the system protein hydrolysates free,
1000 chemically defined and for
development. Within the last 4 high cell density cultivation
of a variety of cell lines such
years the product yield could be 500 as CHO (Chinese Hamster
All graphics created by quadrupled up to 2.3 g/L using Ovary) cells or BHK (Baby
Hamster Kidney) cells and
the MAM-PF77® medium and FMS3 1998 2000 2002 2004 2006 2008 2010 2012 2014 2016
the high level expression
of recombinant proteins.
in a fed-batch. Year BioConcept holds a
MAM-PF®
certificate for every single
component used in the
Increase of Erythropoietin (EPO) yields during the system development. Within the last 4 years the product yield MAM-PF® media series to
could be quadrupled up to 2.3 g/L using the MAM-PF77® medium and FMS3 in a fed-batch. guarantee an untainted and
exceptional final product.
3
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