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Contents Submitting Samples 3 Our Pathology Team 4-5 Top-Up Testing 6-7 At-a-glance Profile Table 8-9 Biochemistry Tests 10 - 11 Additional Biochemistry Tests 12 - 13 Autoimmune Disease 13 Haematology 14 Thyroid Endocrinology 15 Adrenocortical Endocrinology 16 - 17 Reproductive Endocrinology 18 - 19 Therapeutic Drug Monitoring & Toxicology 20 General Microbiology 21 Faecal Analysis 22 - 24 Dermatology Testing 24 Urinalysis 25 Infectious Disease 26 - 32 PCR Testing 33- 34 Allergy Testing & Immunotherapy 35 - 37 Cytology 38 - 40 Histopathology 41 - 43 Exotic Animal Services 44 - 45 MiLab in-house analysers 46 - 51 Index 52 - 61 Terms & Conditions 62 - 63 2 email@finnpathologists.com
Handy Hints and Tips Handy Hints and Tips 1. Please ensure patient details and a succinct clinical history are included on the submission form. 2. Ensure antibiotic treatment is detailed on microbiology submissions. 3. Submitting a fresh blood smear helps us give you the best haematology service. Sample Checks Before Referral 1. Check that the correct tube is used for the test (see below and also refer to our price guide) 2. Check the sample volume required for a specific test and if in doubt please phone us. 3. For all anti-coagulated tubes mix gently but thoroughly to prevent clotting. 4. Use a small wooden stick to check for clots in EDTA samples by gently stirring and scooping the blood. 5. If a plain/gel sample is to be centrifuged before being submitted check that the sample has clotted before centrifugation to avoid a ‘jelly’ supernatant. IMPORTANT NOTE: Samples should be sent in appropriate packaging not only to ensure best quality of result but also to ensure the wellbeing of those handling these packages. If you are unsure on how best to package your sample, please do not hesitate to contact the team or see our guides on our website. Please be aware that making inappropriately packaged samples safe to handle adds extra time and cost to the process and persistent receipt of such packages may incur a Safe Handling charge. We will always consult you in such instances. FINN Blood Tube Identification EDTA LITHIUM HEPARIN 1.3ml (pink top) 1.3ml (orange top) For haematology EDTA is best for Heparin is an anticoagulant preservation of cell morphology. which prevents clotting. EDTA is the sample tube of choice for cytology RED CELLS are NOT needed for the vast majority (with a plain tube if culture is required). of biochemical tests and presence may lead to Please fill to the fill line. haemolysis. Whole heparin is the preferred sample for short samples, e.g. exotics and fractious cats, as it can be used for PLAIN/ SERUM cell counts with a fresh film. Heparin is not as good as EDTA 1.3ml (white top) for preservation of cell morphology. Allow the sample to clot, then centrifuge and separate serum from the clot SODIUM CITRATE and decant into a new plain tube prior to sending. 1.3ml (green top) RED CELLS are NOT needed for the vast majority of Used for coagulation testing. biochemical tests and presence may lead to haemolysis It is essential that this tube is correctly filled as the anticoagulant to GEL BARRIER 1.3ml blood ratio is crucial. The sample may be (brown top) centrifuged and separated citrated plasma Allow the sample to clot, placed in a plain tube. This must then be then centrifuge. The gel sits between clearly stated on the form and tube. May also be the cells and serum protecting the serum from used for D-Dimer. Separated and frozen citrated any haemolysis. The gel may interfere through possible plasma must be used for vWF testing. absorption with the concentration of certain hormones. Some therapeutic drug concentrations are reduced when a sample is stored in a gel tube for greater than 2 hours. Telephone: 01379 854180 3
Finn Pathologists Pathology Team Melanie Dobromylskyj Mark Wessels David Shearer Annalize Ide (Head of Histopathology) (Deputy Head) BVetMed PhD CertSAD BVSc (Hons) MMedVet BSc (Hons), BVSc PhD, BVetMed (Hons), PGCert(VetEd) FHEA CBiol (Path) MRCVS FRCPath MRCVS FRCPath MRCVS MRSB MRCVS Ann Pocknell Joyce Bass Ladislav Novotny Tom Vicek DVM MVetSci DipRCPath BVSc (Pret) MMedVet MVDr, PhD, MSc, FRCPath, DVM PhD Dipl ACVP DipACVP MRCVS (Path) MRCVS MRCVS MRCVS Chris Knott Maria Jose Pinilla Richard Fox Johan Vorster BVSc Cert LAS MRCVS DVM MVETMED MRCVS BVetMed DipECVP MRCVS BVSc MMedVet(Path) Nick Woodger Alistair Cox Debra Fews Gordon Davidson BSc BVet Med FRCPath BVMS, MSc, MRCVS, BSc BVM&S Cert VA Dip (Resident Histopathologist) MRCVS FRCPath RCPath FRCPath FHEA BVMS MRCVS MRCVS RCVS 4 email@finnpathologists.com
Viviana Le Donne Veterinary Consultants DVM, MRCVS, PhD, Diplomate ACVP Susie Kenefick Simone Knudsen MA VetMB DiplECVIM- DVM MRCVS CA MRCVS Susan McLeish BVSc MA (BLT) MSc MRCVS Rachel Millar Barbara Gallagher BVMS CertSAM MRCVS MVB MRCVS DECVIM-CA John Dunn BVM&S MVetSci MA DSAM DiplECVIM DiplECVCP FRCPath MRCVS Jelena Ristic Elizabeth H. Normand BVetMed DSAM CertVC BVSc CertSAM MRCVS MRCVS Jane Miller BVetMed FRCPath MRCVS Frances Dunn Ellie Mardell BVSc MRCVS Cert SAM MA VetMB DSAM (feline) MRCVS RCVS Recognised Specialist in Feline Medicine Guy Davies (Resident Clinical Pathologist) BSC Vet Path (Hons) BVetMed (Hons) AMRSB MRCVS Telephone: 01379 854180 5
Top-Up Testing Any of the tests on these two pages can be ordered at a discounted (Top-Up) rate when ordered in conjunction with one of the chemistry profiles found on pages 10 & 11. This discounted rate only applies if requested at the same time as ordering the chemistry profile. Subsequent requests for additional testing will be at the list price. Page Top-Up Profile Test Price Code no. Price CHEMISTRY & ENDOCRINOLOGY Please specifiy one or more from: Albumin, ALP, ALT, Amylase, BHB (Ketones) Total Bilirubin, Calcium, Cholesterol, Individual Chemistry Chloride, Creatinine, CK, DGGR Lipase, GGT, GLDH, Glucose, 12 Tests Magnesium, Phosphate, Potassium, Protein, Sodium, Triglycerides, Urea, Uric Acid ACTH Stimulation Test Pre + Post samples required (see protocol on page 16) 16 TOPACTC Canine Bile Acid (Basal) 12 TOPBILE Bile Acid Stimulation Pre + Post samples required (see protocol on page 12) 12 TOPBAST Test Basic Thyroid Profile - TOPT4TSH/ 15 Canine TOPFET4TSH Electrolyte Profile 1 12 TOPELEC TOPFRUCFE/ Fructosamine 18 K9 Please note that Top-Up Ionised Calcium can only be requested at the time of submission and only if the appropriate sample Ionised Calcium 12 TOPIONCA type has been received, otherwise result will be inaccurate. See page 12 for sampling protocol. Phenobarbitone 20 TOPBARB Canine Pancreatic 13 TOPCPSP Screening Package Feline Pancreatic 13 TOPFPSP Screening Package Serum Cortisol 16 TOPCORT Canine B12 & Folate 13 TOPB12FK9 Feline B12 & Folate 13 TOPB12FFEL TLI*, B12 & Folate * A fasted blood sample is required for TLI test 13 TOPTLBFLK9 Canine Total T4 15 TOPT4 TOPTSH/ TSH 15 TOPFETSH Free T4 Free T4 by equilibrium dialysis 15 TOPFREET4 SDMAR† (Symmetric dimethylarginine)R† 12 TOPSDMA R = Referral Test †= Non-discountable Test Top-Up Testing If you run a minimum of a pre-surgical profile or more on your MiLab analyser, please attach the results to your submission to be eligible for any of the top-up tests from these two pages at the discounted top-up rate. 6 email@finnpathologists.com
Top-Up Testing Page Top-Up Profile Test Price Code no. Price HAEMATOLOGY Comprehensive Full blood count, platelets and WBC differential count 14 HAEM Haematology Coagulation profile PT and APTT 14 TOPCOAG Coombs Test Investigation of immune-mediated haemolytic anaemia 14 TOPCOOMB MICROBIOLOGY Aerobic Culture 21 TOPMICR1 Comprehensive General aerobic/anaerobic/fungal cultures as necessary. 21 TOPMICR3 Culture Identification and sensitivity Giardia 23 TOPGIAR Giardia & 23 TOPGRDCR Cryptosporidium Lungworm Baermann's test for lungworm 24 TOPFALW Faecal Profile 1 Selective culture for salmonella & campylobacter 23 TOPFA01 Faecal Profile 1 plus worm egg screen (does not include giardia & Faecal Profile 2 23 TOPFA02 cryptosporidium) Faecal Profile 3 Faecal Profile 2 plus testing for giardia & cryptosporidium 23 TOPFA03 Faecal Profile 3 plus testing for parvovirus (comprehensive faecal Faecal Profile 4 23 TOPFA04 analysis for puppies and kittens) URINALYSIS Protein : Creatinine 25 TOPURPC Ratio Cortisol : Creatinine 25 TOPURCC Ratio Urinalysis 1 Urine biochemistry dipstick, microscopy and specific gravity 25 TOPUR01 Urinalysis 1 PLUS Urinalysis 1 plus protein : creatinine ratio 25 TOPUR01+ Urine biochemistry dipstick, microscopy, specific gravity and Urinalysis 2 25 TOPUR02 aerobic culture with sensitivity Urinalysis 2 PLUS Urinalysis 2 plus protein : creatinine ratio 25 TOPUR02+ Urine biochemistry dipstick, microscopy, specific gravity and Urinalysis 3 25 TOPUR03 aerobic culture with sensitivity and cytology Urinalysis 3 PLUS Urinalysis 3 plus protein : creatinine ratio 25 TOPUR03+ Telephone: 01379 854180 7
Biochemistry Profiles at-a-glance Diagnostic Chemistry Diagnostic Chemistry Liver Profile plus Bile Biochemistry Polydipsia Profile PetScreen Profile Senior PetScreen Acid Stimulation Vomiting Profile Standard Profile Pre-anaesthetic Geriatric Profile Renal Profile Basic Profile Liver Profile Profiles Screen 1 Screen 2 Profile Profile PETSCN SNPETSCN POLYK9/ Code AVC03 BAS STANP GERI LIVP LIVP+ VOMP RENP DIAG1 DIAG2 + HAEM + HAEM FE A:G Ratio l l l l l l l l l l l l l ACTH Stimulation Albumin l l l l l l l l l l l l l ALP l l l l l l l l l l l l ALT l l l l l l l l l l l l Amylase l l l AST l l B12 & Folate BHB Bile Acid Stimulation l Bile Acids l l l l l l l Calcium l l l l l l l l l Chloride l l l l l l l l l Cholesterol l l l l l l l l CK l l l l Creatinine l l l l l l l l l l l DGGR Lipase l l l Faecal Analysis FeLV FIV Fructosamine l GGT l l l l GLDH l l Globulin l l l l l l l l l l l l l Glucose l l l l l l l l l l Haematology Comprehensive l l l Magnesium Phosphate l l l l l l l Potassium l l l l l l l l l Sodium l l l l l l l l l Sodium : Potassium Ratio l l l l l l l l l T4 l l TLI Total Bilirubin l l l l l l l Total Protein l l l l l l l l l l l l l Triglycerides l l l l Troponin I TSH Urea l l l l l l l l l l l l l Basic Urine Screen l l Price 2020 R = Referral Test †= Non-discountable Test 8 email@finnpathologists.com
Biochemistry Profiles at-a-glance Intestinal Blood Test Dermatology Blood Intestinal Profile 1 Biochemistry Cardiac Profile Seizure Profile Profiles Screen Profile Code SEIZPK9/FE DBSFT4+ INTP01 INTBLO CARMON A:G Ratio l l l l ACTH Stimulation Albumin l l l l l ALP l l l l ALT l l l l Amylase l AST l l l B12 & Folate BHB Bile Acid Stimulation Bile Acids l l l l Calcium l l l Chloride l l l Cholesterol l l l l CK l l l Creatinine l l l l DGGR Lipase l Faecal Analysis l FeLV FIV Fructosamine l GGT l l GLDH l l Globulin l l l l Glucose l l l l Haematology Comprehensive Magnesium Phosphate l l l Potassium l l l Sodium l l l Sodium : Potassium Ratio l l l T4 l TLI Total Bilirubin l l Total Protein l l l l l Triglycerides l l Troponin I l TSH Urea l l l l Price 2020 R = Referral Test †= Non-discountable Test Telephone: 01379 854180 9
Biochemistry Sample Type Turn Around Profile Test Price Code Required Time BIOCHEMISTRY PROFILES Total protein, albumin, globulin, A:G ratio, urea, creatinine, Pre-anaesthetic Profile 0.5ml Serum Same Day AVC03 ALP, ALT Total protein, albumin, globulin, A:G ratio, urea, creatinine, Basic Profile 0.5ml Serum Same Day BAS ALP, ALT, glucose, total bilirubin and cholesterol Basic Profile plus sodium, potassium, Na:K ratio, chloride, Standard Profile 0.5ml Serum Same Day STANP calcium, bile acids, CK and phosphate Geriatric Profile Standard Profile plus T4 1ml Serum Same Day GERI Total protein, albumin, globulin, A:G ratio, urea, creatinine, 0.5ml Serum PETSCN PetScreen Profile ALP, ALT, calcium, sodium, potassium, Na:K ratio, phosphate, Same Day 1ml EDTA +HAEM chloride, glucose and comprehensive haematology Total protein, albumin, globulin, A:G ratio, urea, creatinine, Senior PetScreen 1ml Serum 1ml SNPETSCN ALP, ALT, calcium, sodium, potassium, Na:K ratio, phosphate, Same Day Profile EDTA +HAEM chloride, glucose and comprehensive haematology and T4 Total protein, albumin, globulin, A:G ratio, ALP, ALT, AST, GGT, Liver Profile 0.5ml Serum Same Day LIVP GLDH, cholesterol, total bilirubin, bile acids and urea Liver Profile plus Bile 1ml Serum x 2 Liver Profile plus bile acid stimulation test Same Day LIVP+ Acid Stimulation (Pre and Post) Sodium, potassium, Na:K ratio, chloride, total protein, albumin, Vomiting Profile globulin, A:G ratio, urea, creatinine, DGGR lipase, glucose, ALP, 0.5ml Serum Same Day VOMP ALT, calcium, bile acids, triglycerides and amylase Sodium, potassium, Na:K ratio, chloride, total protein, albumin, Polydipsia Profile globulin, A:G ratio, urea, creatinine, glucose, ALP, ALT, calcium, 0.5ml Serum Same Day POLYK9/FE bile acids, cholesterol, triglycerides and fructosamine Sodium, potassium, Na:K ratio, chloride, calcium, total Renal Profile protein, phosphate, albumin, globulin, A:G ratio, urea, 0.5ml Serum Same Day RENP creatinine and glucose Sodium, potassium, Na:K ratio, chloride, calcium, phosphate, total protein, albumin, globulin, A:G ratio, urea, creatinine, Seizure Profile 0.5ml Serum Same Day SEIZPK9/FE glucose, cholesterol, bile acids, ALP, ALT, AST, CK and fructosamine DIAGNOSTIC CHEMISTRY SCREENS Albumin, A:G ratio; ALP, ALT, amylase, bile acid (single), Partial Report calcium, chloride, cholesterol, CK, creatinine, DGGR lipase Diagnostic Chemistry 0.5ml Serum Same Day, GGT, globulin, glucose, phosphate, potassium, sodium, Na:K Screen 1 (Biochemistry and Plain Urine Culture DIAG1 ratio, total bilirubin, total protein, triglycerides, urea and Basic + Basic Urine Screen) ** Report up to Urine Screen (UR02- urine biochemistry dipstick, microscopy, 3 Days specific gravity & culture)** Diagnostic Chemistry 0.5ml Serum, Partial Report Screen 2 (Biochemistry Plain Urine, 1ml Same Day, Diagnostic Chemistry Screen 1 plus Comprehensive + Basic Urine Screen EDTA and Air Culture DIAG2 Haematology** + Comprehensive Dried Smear Report up to Haematology) ** 3 Days Glucose Testing: Please note that a spun gel/separated serum sample is the preferred sample for glucose measurement. Spun gel/serum separation should be performed within half an hour of taking the sample. However if using Fluoride Oxalate the tube MUST be filled to the line. **To qualify for the package price, all required samples must be sent at the time of submission. Samples that follow will be charged at the list price. Add Haematology to any of the profiles on these pages for an additional Quote laboratory code 0.5ml EDTA and an air dried smear required Same day turnaround HAEM Include a veterinary Please ensure you provide a full clinical history if interpretation is required. If not ticked interpretation for an on the submission form, but you wish to discuss the results of a case with a Veterinary additional Consultant at a later point, a charge will be added for this phone call. 10 email@finnpathologists.com
Additional Biochemistry Sample Type Turn Around Profile Test Price Code Required Time BIOCHEMISTRY PROFILES CONTINUED Dermatology Blood Total protein, albumin, urea, creatinine, ALP, ALT, 1ml Serum Same Day DBSFT4+ Screen glucose, cholesterol, bile acids and T4 Chemistry 0.5ml Serum Total protein, albumin, globulin, A:G ratio, faecal Same Day, not Heparin & Intestinal Profile 1 analysis (microscopy and worm egg screen, Culture INTP01 Faeces selective culture for Salmonella & Campylobacter) Report up to ** 3 Days Total protein, albumin, globulin, A:G ratio, urea, Chemistry creatinine, ALT, ALP, total bilirubin, amylase, sodium, Intestinal Blood Test 1ml Serum Same Day, potassium, Na:K ratio, calcium, glucose, AST, GLDH, INTBLO Profile not Heparin Final Report GGT, bile acids, cholesterol, triglycerides, DGGR up to 5 Days lipase, chloride, phosphate, CK It can be useful to add a B12 & Folate onto the Intestinal Profile 1 & Intestinal Blood Test Profile when investigating cases of chronic small intestinal diarrhoea. A TLI can also be used to investigate for EPI. A starved serum sample is required. See test codes below. Top-Up Canine B12 and Folate TOPB12FK9 Top-Up Feline B12 and Folate TOPB12FFEL Top-Up Canine TLI, B12 and Folate TOPTLBFLK9 0.5ml Serum Feline TLIR† Up to 7 Days TLIFEL not Heparin Total protein, albumin, globulin, A:G ratio, urea, Part Report creatinine, ALT, ALP, bilirubin, sodium, potassium, 1ml Serum Same Day, Cardiac Profile Na:K ratio, calcium, glucose, AST, GLDH, GGT, CARMON not Serum Gel Final Report bile acids, cholesterol, triglycerides, chloride, up to 4 Days phosphate, CK, troponin I Canine/Feline NT- It may be useful to request this test in conjunction 0.5ml EDTA Up to 7 Days NT-PROBNP proBNP®R† with the Cardiac Profile above plasma PROTOCOL: NT PROBNP SAMPLE HANDLING REQUIREMENTS 1. Draw blood into a syringe. 2. Transfer into standard EDTA tubes. 3. Centrifuge immediately to obtain EDTA plasma. 4. Transfer the plasma into a PLAIN tube ensuring a minimum of 0.5ml & label EDTA plasma. 5. Sample should be kept refrigerated before sending to the lab Samples can be sent to the lab at ambient temperatures but must arrive within 24hrs of sampling. If there is likely to be a delay the samples should be frozen before sending. Frozen samples can be sent to the lab in normal packaging. Glucose Testing: Please note that a spun gel/separated serum sample is the preferred sample for glucose measurement. Spun gel/serum separation should be performed within half an hour of taking the sample. However if using Fluoride Oxalate the tube MUST be filled to the line. R = Referral Test †= Non-discountable Test * A fasted blood sample is required for TLI test. **To qualify for the package price, all required samples must be sent at the time of submission. Samples that follow will be charged at the list price. Telephone: 01379 854180 11
Additional Biochemistry Turn Around Profile Test Sample Type Required Price Code Time Bile Acid (Single) 0.5ml Serum Same Day BILE Top-Up Bile Acid (Single) TOPBILE Bile Acid Stimulation Bile acid x 2 (see protocol below) and total 0.5ml Serum x 2 Same Day BAST Test bilirubin Top-Up Bile Acid Stimulation Test TOPBAST PROTOCOL: BILE ACID STIMULATION 1. Starve the patient for at least 12 hours. 2. Collect first sample into a serum gel or plain tube. 3. Feed a high fat meal (suggested meals include puppy/kitten food or recovery diet). The addition of oil is not usually necessary and reduces palatability. 4. Collect a second sample 2 hours post feeding into a serum and gel or plain tube. Request bile acid stimulation testing. Label tubes with client details and the times the samples were taken. Please specifiy one or more from: Albumin, ALP, ALT, Amylase, BHB (Ketones) Total Individual Chemistry Bilirubin, Calcium, Cholesterol, Chloride, Creatinine, CK, DGGR Lipase, GGT, GLDH, Same Day Tests Glucose, Magnesium, Phosphate, Potassium, Protein, Sodium, Triglycerides, Urea, Uric Acid Top-Up Individual Chemistry Tests Electrolyte Profile 1 Sodium, potassium, Na:K ratio and chloride 0.5ml Serum Same Day ELEC1 Top-Up Electrolyte 1 TOPELEC Electrolyte Profile 2 Calcium, phosphate and Ca:P ratio 0.5ml Serum Same Day ELEC2 Ionised Calcium See Protocol Below Same Day IONCA Top-Up Ionised Calcium TOPIONCA PROTOCOL: IONISED CALCIUM 1. Collect the blood into a serum tube (not serum gel), ensure the tube is 100% full and avoid the inclusion of air bubbles. 2. Allow 15 minutes for the blood to clot then centrifuge at high speed, remove the serum and place into a completely filled airtight tube. 3. We can provide tubes that hold 0.5ml making it easier to fill completely. Please contact the lab to order. Label tube with client details. SDMA (Symmetric Used to detect the early stages of renal 0.5ml Separated Serum or Up to 3 Days SDMA dimethylarginine)R† dysfunction in dogs and cats. 0.5ml Heparin Plasma Top-Up SDMAR† (Symmetric dimethylarginine)R TOPSDMA Alpha 1 AGP † R 0.5ml Serum/Heparin Plasma Up to 7 Days ALPHA Canine Prostate Specific Arginine Prostate hyperplasia 0.5ml Serum Up to 8 Days ODEL Esterase (CPSE)R† CRP (C-Reactive Canine only 0.5ml Serum Same Day CRP Protein) Serum Amyloid A (SAA)R† 0.5ml Serum Up to 4 Days SAA LDH 0.5ml Serum Up to 4 Days LDH Iron & Total Iron Binding Capacity CombinedR† 1ml Serum Up to 7 Days IRTB LeadR† 1ml whole EDTA/Heparin Up to 5 Days PB CopperR† 0.5ml Heparin Plasma Up to 3 Days CU Up to 2 TaurineR† Cannot run on a haemolysed sample 1ml Serum TAUR Weeks Zinc free tubes are available on request, ZincR† alternatively avoid use of tubes with rubber 0.5ml Serum Up to 4 Days ZINC bungs. R = Referral Test †= Non-discountable Test 12 email@finnpathologists.com
Additional Biochemistry Sample Type Profile Test Turn Around Time Price Code Required Canine/Feline 0.5ml EDTA Please refer to protocol below Up to 7 Days NT-PROBNP NT-proBNP®R† plasma PROTOCOL: NT PROBNP SAMPLE HANDLING REQUIREMENTS 1. Draw blood into a syringe. 2. Transfer into standard EDTA tubes. 3. Centrifuge immediately to obtain EDTA plasma. 4. Transfer the plasma into a PLAIN tube ensuring a minimum of 0.5ml & label EDTA plasma. 5. Sample should be kept refrigerated before sending to the lab Samples can be sent to the lab at ambient temperatures but must arrive within 24hrs of sampling. If there is likely to be a delay the samples should be frozen before sending. Frozen samples can be sent to the lab in normal packaging. 0.5ml Serum Troponin I Up to 4 Days TROPI not Serum Gel Serum Protein 0.5ml Serum Up to 5 Days AXSPE Electrophoresis 0.5ml Serum Canine TLI Fasted blood sample required Same Day TLIK9 not Heparin 0.5ml Serum Feline TLIR† Fasted blood sample required Up to 7 Days TLIFEL not Heparin B12 0.5ml Serum Same Day B12 Folate 0.5ml Serum Same Day FOLV Canine B12 and Folate 0.5ml Serum Same Day B12FK9 Top-Up Canine B12 and Folate TOPB12FK9 Feline B12 and Folate 0.5ml Serum Same Day B12FFEL Top-Up Feline B12 and Folate TOPB12FFEL 0.5ml Serum Canine TLI, B12 and Folate Fasted blood sample required for TLI test Same Day TLBFLK9 not Heparin Top-Up Canine TLI, B12 and Folate TOPTLBFLK9 PANCREATIC SCREENING PACKAGE This package consists of an initial screening test which will be performed on the day of receipt and reported as positive, negative or equivocal. Any equivocal results will be sent away to a referral lab for cPLI or fPLI (at no additional cost) and a follow up report issued once received. Same Day / Follow up Canine Pancreatic 1ml Serum Fasted blood sample required Report (If Required) up CPSP Screening Package not Heparin to 3 Days Top-Up Canine Pancreatic Screening Package TOPCPSP Same Day / Follow up Feline Pancreatic 1ml Serum Fasted blood sample required Report (If Required) up FPSP Screening Package not Heparin to 3 Days Top-Up Feline Pancreatic Screening Package TOPFPSP Serum Osmolarity † R 0.5ml Serum Up to 5 Days SOSMO AUTOIMMUNE DISEASE Anti-nuclear Antibodies 0.1ml Serum Up to 7 Days ANA Acetylcholine Receptor AntibodiesR† 0.3ml Serum Up to 14 Days CSACRAB Muscle Fibre Antibody Type 2MR† 0.3ml Serum Up to 21 Days CS2MAB Rheumatoid Factor Waaler-Rose 0.1ml Serum Up to 2 Days ROSE R = Referral Test †= Non-discountable Test Include a veterinary Please ensure you provide a full clinical history if interpretation is required. If not ticked interpretation for an on the submission form, but you wish to discuss the results of a case with a Veterinary additional Consultant at a later point, a charge will be added for this phone call. Telephone: 01379 854180 13
Haematology Test Turn Around Profile Sample Type Required Price Code Please submit relevant clinical history Time Clinical Pathologist haematology blood Pathologist Haematology smear review and report. This can be *Same Day PHR Review added to comprehensive haematology submissions only *Please note that requests received after midday cannot be guaranteed to be fulfilled on the same day. Differential white blood cell count and Blood Smear Examination smear exam performed by a highly Air Dried Smear Same Day HAEMEX Only trained haematology technician Comprehensive Haematology Full blood count, platelets and WBC 0.5ml EDTA & Air Dried Smear Same Day HAEMO only (Canine, Feline, Equine) differential count Comprehensive Haematology Hb, PCV, WBC Manual differential count, 0.5ml EDTA/ Heparin & Air Dried Up to 3 Days HAEMAV only (Exotics, Avians) RBC and Platelet comment Smear Comprehensive Haematology Comprehensive Haematology added to a 0.5ml EDTA & Air Dried Smear Same Day HAEM with profile Biochemistry Profile Comprehensive Haematology Comprehensive Haematology added to a 0.5ml EDTA/ Heparin & Air Dried Up to 3 Days HAEMAV+ with profile (Exotics, Avians) Biochemistry Profile Smear HAEMATOLOGY SAMPLING When obtaining samples for haematology testing, where possible, attempt to obtain sufficient sample to fill sample tubes to the indicated "fill line". With all reptile and some avian species, blood for haematology must be supplied in heparin tubes. FILM REPORT Where abnormalities are present, and/or clinical history indicates, a manual WBC differential and film review will be performed by a highly trained haematology technician. Reticulocyte counts are added in all feline anaemia cases. Investigation of immune-mediated Coombs Test 0.5ml EDTA & Air Dried Smear Same Day COOMB haemolytic anaemia Top-Up Coombs Test TOPCOOMB Manual PCV Packed cell volume 0.2ml EDTA Same Day PCV Reticulocyte Count 0.5ml EDTA & Air Dried Smear Same Day RET Buffy Coat Smear Examination 0.5ml EDTA Same Day BUFF Platelet Count Platelet count + assessment 0.5ml EDTA Same Day PLC Fibrinogen By heat precipitation 0.5ml EDTA Same Day FIB Functional assay based on the time for fibrin clot formation, more sensitive for Fibrinogen, Clauss Method Citrate Tube Filled to Line 1 Day CFIBR low fibrinogen levels compared with the heat precipitation method Prothrombin Time Single PT Citrate Tube Filled to Line Same Day PT Coagulation Profile PT and APTT Citrate Tube Filled to Line Same Day COAG Top-Up Coagulation Profile TOPCOAG Platelet count + assessment, PT, APTT and Citrate Tube Filled to Line, 0.5ml Extended Coagulation Profile Same Day COAGEX fibrinogen EDTA & Air Dried Smear Haemorrahage Investigation Haemotology, PT, APTT, D-Dimers & 0.5ml Whole EDTA, Air Dried 1 Day HAEMOC Comprehensive Fibinogen (Clauss) Smear & Citrate Tube Filled To Line PT: Test to check the extrinsic coagulation pathway, to diagnose vitamin K antagonist toxicity and monitor therapy and to test for hepatic associated coagulation disorders. APTT: Test to check the intrinsic coagulation pathway, to diagnose factor VIII, IX, XI and XII deficiencies and to monitor heparin therapy. Von Willebrand's Factor 0.5ml Citrate Plasma See protocol below Up to 5 Days VWF TestingR† FROZEN PROTOCOL: VON WILLEBRAND’S FACTOR SAMPLE HANDLING REQUIREMENTS 1. Draw blood into a syringe. 2. Transfer into standard Sodium Citrate tube (filled to line). 3. Centrifuge immediately to obtain citrate plasma. 4. Transfer the plasma into a PLAIN tube ensuring a minimum of 0.5ml & label Citrate plasma. 5. Freeze samples immediately. Samples need to arrive at the laboratory frozen. Please contact us to request a cool box and ensure that you follow the instructions carefully. D Dimers (Canine) Citrate Tube Filled to Line 1 Day DDIME Blood Groups (Canine/Feline) 0.5ml Whole Blood EDTA 1 Day BLGR R = Referral Test †= Non-discountable Test 14 email@finnpathologists.com
Thyroid Endocrinology Sample Type Turn Around Profile Test Price Code Required Time First line test for diagnosis of hyperthyroidism Total T4 (Canine/Feline) 0.5ml Serum Same Day TT4 in felines Top-Up Total T4 (Canine/Feline) TOPT4 TSH (Canine) Test for canine thyroid stimulating hormone 0.5ml Serum Same Day TSH Top-Up TSH (Canine) TOPTSH TSH (Feline) 0.5ml Serum Same Day FETSH Top-Up TSH (Feline) TOPFETSH Basic Thyroid Profile (Canine) Total T4 and TSH (Canine) 0.5ml Serum Same Day T4TSH Top-Up Basic Thyroid Profile TOPT4TSH Basic Thyroid Profile (Feline) Total T4 and TSH (Feline) 0.5ml Serum Same Day FET4TSH Top-Up Basic Thyroid Profile TOPFET4TSH CANINE HYPOTHYROIDISM MONITORING Soloxine, Leventa, Thyroxyl: collect sample 4-6 hours post pill. Thyforon: collect sample 3 hours post pill. Trough TT4 (immediately pre pill) can be useful in certain cases. We recommend the Basic Thyroid Profile above. 0.5ml Serum Free T4 Free T4 by equilibrium dialysis Up to 5 Days FREET4 not heparin Top-Up Free T4 TOPFREET4 Total T3R† 1ml Serum Up to 3 Days TT3 Thyroglobulin Testing for the presence of thyroglobulin 0.5ml Serum Up to 8 Days TGAA Autoantibodies (TGAA) autoantibody (canine only) TSH Same Day Silver Thyroid Profile 1ml Serum TSH and Free T4 Free T4 up to 5 THYSIL (canine only) not heparin Days T4/TSH Same Day Platinum Thyroid Profile 1ml Serum Total T4, TSH, Free T4 and TGAA Final Report up THYPLAT (canine only) not heparin to 8 Days T4 Same Day Feline Hyperthyroid 1ml Serum Total T4 and Free T4 Free T4 up to 5 FEHYTH Diagnostic Profile not heparin Days Feline Hyperthyroid Total T4, urea, creatinine, ALT, ALP, calcium, 0.5ml Serum Same Day FE-HYPERT Monitoring Profile phosphate PARATHYROID ENDOCRINOLOGY 0.5ml EDTA Plasma Used to investigate persistant hypo or Parathyroid Hormone (PTH)R† Only (Frozen) - Do Not Up To 7 Days PTH hypercalcaemia Freeze Whole EDTA Used to investigate persistent hypercalcaemia. 0.5ml EDTA Plasma PTHrPR† Increased levels usually associated with Only (Frozen) - Do Not Up To 7 Days PTHRP malignancy. (Note: specific sample requirements) Freeze Whole EDTA Please contact the lab for sampling protocol and cool box. Interpretation of PTH & PTHrP requires concurrent measurement of serum Calcium & Ionised Calcium. Prices & sampling requirements for these tests can be found on page 12. PARATHYROID HORMONE RELATED PEPTIDE (PTHrP) • Collect blood into plastic chilled EDTA tube - DO NOT fill completely. • Mix very well but gently by inversion. • Centrifuge as quickly as possible (ideally in a refrigerated centrifuge). • Transfer the plasma into a cooled plastic (not glass) PLAIN tube kept on ice. • Immediately freeze (< -10°C) the plasma sample and keep frozen until dispatch in the transport pack. • Contact lab to request a cool box. Sample MUST arrive at lab frozen. Indications & Uses: Investigation of persistent hypercalcaemia. Increased levels usually associated with malignancy. Interpretation requires concurrent measurement of serum Calcium and Ionised Calcium: prices and sampling requirements on pages 12. Add Haematology to any of the profiles above (THYSIL, THYPLAT, FEHYTH, FEHYPERT) for an additional Quote laboratory code 0.5ml EDTA and an air dried smear required Same day turnaround HAEM Telephone: 01379 854180 15
Adrenocortical Endocrinology Sample Type Turn Around Profile Test Price Code Required Time Used as a screening test for hyperadrenocorticism. The urine sample must be collected first thing in Urine (plain Cortisol:Creatinine Ratio Same Day URCC the morning, pre-feeding and must be from a non- universal) stressed animal. Top-Up Cortisol:Creatinine Ratio TOPURCC Used as a screening test for monitoring hypoadrenocorticism or for the monitoring Serum Cortisol 0.5ml Serum Same Day CORT of hyperadrenocorticism pre-trilostane administration. Top-Up Serum Cortisol TOPCORT Cortisol x 2. Pre & post samples required Canine ACTH Stimulation Test 0.5ml Serum x 2 Same Day ACTHK9 (see protocol below) Top-Up Canine ACTH Stimulation Test TOPACTC PROTOCOL: CANINE ACTH STIMULATION TEST Used to help diagnose hyperadrenocorticism (Cushing's) and hypoadrenocorticism (Addison's). 1. Collect a first, baseline sample in a serum gel or plain tube. 2. Inject 0.005mg/kg synthetic ACTH (e.g. Tetracosactide or Synacthen) intravenously or intramuscularly. 3. Collect a second sample 1 hour later. 4. Submit samples together requesting canine ACTH stimulation test. Label tubes with client details and the times the samples were taken. Canine Low-dose Dexametha- Cortisol x 3, Pre + Post samples required 0.5ml Serum Same Day LDDSK9 sone Suppression Test (see protocol below) x3 PROTOCOL: CANINE LOW-DOSE DEXAMETHASONE SUPPRESSION TEST Used to help diagnose spontaneous hyperadrenocorticism. The LDDST is not suitable for the detection of iatrogenic hyperadrenocorticism. 1. Collect a first, baseline sample in a plain tube. 2. Inject 0.015 mg/kg dexamethasone intravenously. 3. Collect a second sample 3 hours post dexamethasone in a plain tube. 4. Collect a third sample 8 hours post dexamethasone in a plain tube. 5. Submit samples together requesting low-dose dexamethasone test. Label tubes with client details and the times the samples were taken. MONITORING OF DOGS ON TRILOSTANE (VETORYL) OPTIONS The clinical picture is very important in decision making about which tests to run and dose changes. Please include as full a current clinical picture as possible. A clinical assessment questionnaire can be found on the Dechra website, which may be of use. Please feel free to call our internal medicine consultants if you would like to discuss further. A sample should be taken pre-pill at the time the Cortisol pre-pill (single tablet is due. The tablet can be postponed for up to 0.5ml Serum Same Day CORT cortisol) 2hrs to allow a sample to be taken pre-pill. Cortisol pre-pill plus Single cortisol, sodium, potassium & sodium 0.5ml Serum Same Day TRILBAS electrolytes potassium ratio Cortisol pre-pill Single cortisol, sodium, potassium, sodium 0.5ml Serum Same Day TRILMON comprehensive potassium ratio, ALP, ALT, urea & creatinine ACTH stimulation test 4 - 6 Cortisol x 2. Pre & post samples required (see 0.5ml Serum x 2 Same Day ACTHK9 hours post-pill protocol above). ACTH stimulation test Cortisol x 2, sodium, potassium & sodium 4-6 hours post-pill plus 0.5ml Serum x 2 Same Day TRIL1 potassium ratio electrolytes ACTH simulation test 4 - 6 Cortisol x2, sodium, potassium, sodium potassium 0.5ml Serum x 2 Same Day VETMON hours post-pill comprehensive ratio, ALP, ALT, urea & creatinine 16 email@finnpathologists.com
Adrenocortical Endocrinology Turn Around Profile Test Sample Type Required Price Code Time CANINE ADDISONS (HYPOADRENOCORTICISM) Once a diagnosis has been obtained and therapy is in progress, adrenal function will be further suppressed by the therapy. An ACTH stimulation test will not provide any further useful information. These cases should be monitored on the basis of electrolyte levels and clinical signs. Addisonian Diagnostic Cortisol x 2, sodium, potassium (+ ratio) Pre + Post 0.5ml Serum x 2 Same Day ADDIS Profile samples required (see protocol on p16) Used to differentiate pituitary dependent Endogenous ACTH hyperadrenocorticism (PDH) from 0.5ml Separated EDTA Up To 3 Days SAEAC Measurement hyperadrenocorticism caused by an adrenal tumour Plasma Only (FROZEN) (AT)/adrenal dependent hyperadrenocorticism (ADH). PROTOCOL: ENDOGENOUS ACTH ASSAY: 1. Place plastic EDTA tubes and syringes in freezer for several minutes prior to sample collection. 2. Collect blood into chilled plastic EDTA tube (do not use glass tubes). 3. Centrifuge immediately. 4. Decant plasma into chilled plain plastic tube. 5. Freeze immediately. 6. Contact lab to request a cool box. 7. Sample MUST arrive at lab frozen. Indications & Uses: Differentiating pituitary dependent HAC from an adrenal tumour, differentiating primary and secondary hypoadrenocorticism. Feline Low-dose Dexameth- Cortisol x 3 Pre + Post asone Suppression Test Used to help diagnose spontaneous samples required (see Same Day LDDSFE (cortisol before and after hyperadrenocorticism. protocol below) 0.5ml dexamethasone) Serum x 3 PROTOCOL: FELINE LOW-DOSE DEXAMETHASONE SUPPRESSION TEST 1. Collect a first, baseline sample in a serum gel or plain tube. 2. Inject 0.1mg/kg dexamethasone intravenously. 3. Collect further samples at 3-4 hours and 8 hours after dexamethasone injection. 4. Submit samples together requesting low-dose dexamethasone test. Label tubes with client details and the times the samples were taken. Cortisol x 3, Pre + Post samples required (see protocol below). This test can be used to investigate possible Feline ACTH Stimulation hyperadrenocorticism. Note false negatives can 0.5ml Serum x 3 Same Day ACTHFE Test occur with this test. Please phone for advice prior to requesting this test. PROTOCOL: FELINE ACTH STIMULATION TEST Hyperadrenocorticism in cats is rare. This test has a low sensitivity for the detection of hyperadrenocorticism in felines. 1. Collect a first, baseline sample in a serum gel or plain tube. 2. Inject 0.125mg synthetic ACTH (Synacthen) intravenously (0.25mg may be used in cats over 5kg). 3. Collect further blood samples 60 and 90 minutes later (NB: If Synacthen given IM then collect samples at 30 and 60 minutes later. Please note, however, IV is the preferred route for this test in felines). 4. Submit samples together requesting feline ACTH stimulation test. Label tubes with client details and the times the samples were taken. 17-OH Progesterone Pre & Additional test for the investigation of hyperadreno- 0.5ml Serum x2 Up to 4 Days 17OHP Post ACTHR† corticism. Pre & post samples required. For the investigation of hyperaldosteronism and Aldosterone (single)R† 0.5ml Serum Up to 7 Days ALDE sometimes in hypoadrenocorticism Aldosterone before and after ACTH. Additional test Aldosterone (pre & post for the investigation of adrenal diseases. Pre & post 0.5ml Serum x2 Up to 7 Days ALDACT ACTH)R† samples required. 2ml EDTA Plasma ReninR† Lab MUST be contacted before requesting this test. Up to 14 Days RENIN (FROZEN) 0.5ml Serum (NOT Combined Aldosterone and Lab MUST be contacted before requesting this test. FROZEN) 2ml EDTA Up to 14 Days REAL ReninR† Plasma (FROZEN) HYPERALDOSTERONISM Please contact the lab for the protocol and a cool box as there are specific handling requirements for the renin sample. R = Referral Test †= Non-discountable Test Telephone: 01379 854180 17
Reproductive Endocrinology Sample Type Turn Around Profile Test Price Code Required Time ASSESSMENT OF NEUTER STATUS/DETECTION OF OVARIAN REMNANT AT ALL STAGES OF CYCLE (CANINE/FELINE) Sensitive test for detection of gonadal tissue and assessment of neuter Anti-Mullerian status in dogs and cats. Occasional false negatives can occur in dogs 0.5ml Serum Up to 7 Days MULL HormoneR† in the progesterone dominated phase of the cycle, measurement of progesterone can help determine status in such cases. Anti-Mullerian hormone is the test of choice for detecting gonadal tissue and assessment of neuter status (including ovarian remnants) for adult dogs and cats. Other test options can be found below but false negatives can occasionally occur. Occasional false negatives can occur in the progesterone dominant phase of the cycle. Measurement of progesterone can aid interpretation in such cases. OVARIAN REMNANTS (FELINE) IF QUEEN IS SHOWING SIGNS OF OESTRUS Can be used as a first line screen as increased concentrations suggest Basal OestradiolR† that ovarian remnant or oestrogen excess due to adrenocortical 0.5ml Serum Up to 8 Days OEST disease. Dynamic Pre - Same Day Progesterone x 2. Pre + Post hCG samples required (see protocol 0.5ml Serum Progesterone Post - Up to 7 DHCGF below). x2 Stimulation Test Days PROTOCOL: DYNAMIC PROGESTERONE STIMULATION TEST Samples should be taken between day 1 and 3 after the onset of oestrus behaviour. 1. Collect a first, baseline sample in a serum gel or plain tube. Allow to clot and separate serum. 2. Inject 44 iu/kg hCG intramuscularly (please note off licence use). 3. Take a second sample 7 - 14 days later in a plain or serum gel tube. Allow to clot and separate serum. 4. Request progesterone on each sample. Label tubes with client details and the times the samples were taken. OVARIAN REMNANTS (FELINE) IF QUEEN IS REMOTE FROM A PERIOD OF SHOWING SIGNS OF OESTRUS Oestradiol x 2. Pre + Post GnRH samples required (see protocol Dynamic Oestradiol 0.5ml Serum below) Used to identify ovarian remnants in feline and can be used Up to 7 Days GRHSTM Stimulation TestR† x2 when feline is not showing signs of oestrus. PROTOCOL: FELINE DYNAMIC OESTRADIOL STIMULATION TEST 1. Collect a first, baseline sample in a serum gel or plain tube. 2. Inject 2µg/kg Buserelin/synthetic GnRH (Receptal®, Intervet SP) intramuscularly or 44 iu/kg hCG intramuscularly (please note off licence use). 3. Take a second sample 2 hours later in a serum gel or plain tube. 4. Request GnRH Stimulation test Label tubes with client details and the times the samples were taken. OVARIAN REMNANTS (CANINE) IF BITCH IS SHOWING SIGNS OF OESTRUS Screening test for bitches showing signs of oestrus. Some bitches with Progesterone signs of oestrus may have increased progesterone. Progesterone is 0.5ml Serum Same Day PROGK9 (canine) expected to stay high throughout dioestrus (approximately 60 days). Screening test; increased oestradiol is consistent with follicular Basal Oestradiol activity and is supportive of ovarian remnant. Also for investigation 0.5ml Serum Up to 7 Days OESTC (canine)R† of possible Sertoli cell tumour in males. Pre and post hCG 0.5ml Serum Dynamic Oestradiol Oestradiol x 2. Pre + Post hCG samples required. Up to 8 Days DYOES x2 (canine)R† OVARIAN REMNANTS (CANINE) IF BITCH IS REMOTE FROM A PERIOD OF SHOWING SIGNS OF OESTRUS Pre and post hCG False negatives can occassionally occur. Please contact the lab for 0.5ml Serum Dynamic Oestradiol Up to 8 Days DYOES further details. x2 (canine)R† PROTOCOL: PRE AND POST HCG DYNAMIC OESTRADIOL (CANINE) 1. Take basal serum or heparinised plasma sample. 2. Inject 2µg/kg synthetic GnRH (Receptal®, Intervet SP) intramuscularly or 44 iu/kg hCG intramuscularly (please note off licence use). 3. Collect second sample 2 hours later. 4. Oestradiol is measured on both samples. Label tubes with client details and the times the samples were taken. R = Referral Test †= Non-discountable Test 18 email@finnpathologists.com
Reproductive Endocrinology Sample Type Turn Around Profile Test Price Code Required Time PRE-MATE TESTING (CANINE) May be used to help predict ovulation in bitches. Testing is generally recommended to begin from 7 days after the onset of Basal Progesterone 0.5ml Serum Same Day PROGK9 the season. Also used in conjunction with oestradiol to stage oestrus cycle in bitches showing ambiguous signs. CRYPTORCHIDISM (CANINE & FELINE) Screening test. False negatives common. Fluctuations occur during the day. Only Testosterone 0.5ml Serum Up to 4 Days TEST high levels confirm the presence of testicular tissue. Testosterone x 2. Pre + Post hCG samples Dynamic required (see protocol below) If testicular Testosterone 0.5ml Serum x 2 Up to 4 Days CRYTEST tissue is present then a significant rise in Stimulation Test testosterone is detected post stimulation. PROTOCOL: DYNAMIC TESTOSTERONE STIMULATION TEST (For Feline Testosterone please call for advice prior to sampling) 1. Collect a first, baseline sample in a serum tube 2. Inject 2µg/kg synthetic GnRH (Receptal®, Intervet SP) intramuscularly or 44 iu/kg hCG intramuscularly (please note off licence use). 3. Take a second sample 1 - 2 hours post-injection in a serum gel or plain tube. 4. Request testosterone on each sample Label tubes with client details and the times the samples were taken OTHER HORMONES 0.5ml Serum. Allow sample to clot then spin/separate serum Glucose Insulin (RIA) and glucose. An insulin result R within 30 minutes of sample Same Day Insulinoma ProfileR† cannot be interpreted unless there is INSGL collection. Ideally enclose an ice Insulin up to confirmed, concurrent hypoglycaemia. pack, otherwise a small decrease 3 Days in insulin may occur. Fructosamine 0.5ml Serum Same Day FRUCK9/FRUCFE Top-Up Fructosamine TOPFRUCK9/FE Diabetes Profile Fructosamine and glucose 0.5ml Serum Same Day DIABK9/FE IGF-1R† Insulin Like Growth Factor-1 0.5ml Serum 7 to 10 Days IGF1 Glucose Testing: Please note that a spun gel/separated serum sample is the preferred sample for glucose measurement. Spun gel/serum separation should be performed within half an hour of taking the sample. However if using Fluoride Oxalate the tube MUST be filled to the line. R = Referral Test †= Non-discountable Test Telephone: 01379 854180 19
Therapeutic Drug Monitoring & Toxicology Sample Type Turn Around Profile Test Price Code Required Time 0.5ml Serum Phenobarbitone Same Day BARB not Serum Gel Top-Up Phenobarbitone TOPBARB Phenobarbitone Phenobarbitone, ALT, ALP, GGT, albumin and total 0.5ml Serum Same Day PHENOM Monitoring bilirubin not Serum Gel Extended Phenobarbitone 0.5ml Serum Phenobarbitone Monitoring plus AST and bile acids Same Day PHENEX Monitoring not Serum Gel Extended Phenobarbitone Extended Phenobarbitone Monitoring plus cholesterol 0.5ml Serum Same Day PHENEX+ Monitoring Plus and triglycerides not Serum Gel PHENOBARBITONE It is advisable to check hepatic function during therapy. In stable animals sample timing is not critical. In un-stable animals consider a peak and trough sample. 0.5ml Serum Digoxin Up to 4 Days DIG not Serum Gel Part Report Same Digoxin, ALT, ALP, total protein, albumin, globulin, 1ml Serum Digoxin Monitoring Profile Day, Digoxin up to DIGMON urea, creatinine, sodium, potassium (+ ratio) not Serum Gel 4 Days PROTOCOL: DIGOXIN Serum levels, after initial diagnosis, should be given 5-7 days to adjust. A blood sample obtained 3-8 hours post dosing is to determine peak levels. Be aware that in some animals digoxin has a short half life and peak levels may develop within 3 hours of dosing. It is advisable to monitor liver and renal function and Na:K ratio at the same time. 1. Collect a serum sample 3-8 hours post dosing. 2. Label the tube with the patient details. Do not use glass containers with rubber stoppers as it may bind the drug and decrease measured concentrations. 0.5ml Serum Potassium Bromide Up to 4 Days POTB not Serum Gel POTASSIUM BROMIDE (KBR) Potassium Bromide levels do not stabilise until the patient has been on therapy for at least 2-3 months. The same sample taken for monitoring phenobarbitone levels is acceptable for monitoring potassium bromide levels. The timing of the sample is not critical for this assay. Combined Potassium Phenobarbitone 1ml Serum Bromide and Same Day PHBRO not Serum Gel Phenobarbitone KBr up to 4 Days Sample collection 12 hours after last dose for trough, 0.5ml Whole CyclosporinR† Up to 3 Days CYCLO peak 2 - 4 hours post pill. EDTA Keppra (Levetiractetam)R† Sample collected at trough directly before the next dose. 0.5ml Serum Up to 3 Days KEPP TOXICOLOGY We recommend a minimum volume of 2ml whole blood or plasma or serum; 5ml plain urine (10 ml if paraquat and/or diquat required); 10g fresh tissue; 10g feed or contaminated materials; 5ml stomach contents, as applicable. Samples from at least two different sites are preferable as many drugs leave the blood relatively quickly. Samples may be frozen. Formalin fixed tissue or faeces is unsuitable. If insufficient sample is received, it may not be possible to complete all the requested tests and the sensitivity of toxin/drug detection could be reduced. Screens for 30,000 drugs and toxins. Please contact Toxicology PanelR† the lab to check that the panel includes the drugs and See above Up To 7 Days TOXP toxins you require. Toxicology Panel plus Ethylene GlycolR† See above Up To 7 Days TOXPEG Ethylene Glycol † R See above Up To 7 Days ETHG Warfarin and Coumarin DerivativesR† See above Up To 7 Days WCD This test is particularly helpful in cases where blood is the only sample type available and is routinely Vitamin K1/K1 Epoxide † R Whole Heparin Up To 12 Days K1EP recommended for suspected Vitamin K antagonist rodenticide toxicity. For other toxins, please contact the laboratory. R = Referral Test †= Non-discountable Test Include a veterinary Please ensure you provide a full clinical history if interpretation is required. If not ticked interpretation for an on the submission form, but you wish to discuss the results of a case with a Veterinary additional Consultant at a later point, a charge will be added for this phone call. 20 email@finnpathologists.com
General Microbiology Sample Type Turn Around Profile Test Price Code Required Time Aerobic Culture with Sensitivity Tissue/Fluid/Swab 2 to 3 Days MICRO1 Top-Up Routine Aerobic Culture with Sensitivity TOPMICR1 Anaerobic Culture Tissue/Fluid/Swab 2 to 3 Days MICR2 Comprehensive General aerobic/anaerobic/fungal cultures as necessary. Tissue/Fluid/Swab 2 to 3 Days MICR3 Culture Identification and sensitivity Top-Up Comprehensive Culture TOPMICR3 GUIDELINES FOR BACTERIAL CULTURE Please note any information regarding antibiotic treatment, either current or recent, on the submission form. Aerobic and Anaerobic Culture: This would be recommended for most samples submitted. Aerobic Culture: This is suitable for bacteria growing in oxygen-rich areas, such as superficial skin, conjunctiva, ears and urine. Anaerobic Culture: This is suitable for bacteria that can live in the absence of oxygen as with blood, bile, bone marrow, CSF, direct lung aspirates, tissue biopsy or fluid from a normally sterile site (such as joint or body cavity), abscesses, deep wounds and deep skin infections. Susceptibility testing is not performed on organisms usually considered to be commensal to aid with antibiotic governance (except if specifically requested). Sample requirements: Charcoal swabs, plain fluids and fresh tissue are recommended samples. EDTA tubes and viral transport media swabs are not recommended as they can inhibit bacterial growth. Aerobic culture with sensitivity and a Gram stain for Ear Swab Examination Charcoal Swab 2 to 3 Days EARS yeast cells Ear Swab Examination Aerobic culture, basic cytology smear exam & Charcoal Swab & 2 2 to 7 Days EARS+ PLUS microscopic exam for parasites & yeast culture unstained smears Examination for Tissue/Fluid/Swab/ Gram stain examination Same Day GRAM Bacteria/Yeasts Slides Aerobic and anaerobic culture with sensitivity. Please contact the Lab to obtain blood culture bottles Blood Culture/Joint (additional charge) and sampling instructions. Blood Culture Bottle 8 to 10 Days BLOC Fluids Culture of additional bottles for the same patient are charged at 50% of the list price Nocardia/Actinomyces Culture Tissue/Swab 7 to 14 Days NOAC Examination for Ziehl-Neelsen staining for Mycobacteria Tissue Up to 2 Days ZN Mycobacteria A charcoal swab of both nostrils & Screening for MRSA/ Selective culture for screening carriers of methicillin buccal mucosa and 2 to 3 Days MRSA MRSP resistant Staphylococci. a second charcoal swab of the axilla & perineum Routine culture from environmental swabs to help Environmental Environmental monitor hygiene controls. Includes total viable count, Swabs (request from Up to 7 Days MICENV1 Monitoring bacterial identification, yeast and fungal culture, laboratory) selective culture for MRSA/MRSP Mycoplasma Culture Tissue/Fluid/Swab Min of 7 Days MYCOP Mycobacterium Fresh tissue is the preferred sample type, however Fresh Tissue/Pus Up to 6 Weeks MYBAC CultureR† pus/fluid can be submitted if required. Multi-Resistance Screening Panel using Sensititre Microbroth dilution plate. Appropriate for request Aerobic Bacterial MIC Determination† Up to 4 Days MICE following a culture report exhibiting multi-drug Isolate resistant antimicrobial susceptibility results. Identification of a cultured isolate. If you are struggling to identify a bacterial isolate you have cultured in Isolate Identification house, send it to us for a second opinion. Please check Bacterial Isolate 7 to 14 Days IDENT transport regulations before sending cultured isolate or contact the laboratory for further guidance BACTERIAL CULTURE PRICING - MULTIPLE SAMPLES Please note that when submitting samples for any of the above cultures or stains that if submitting more than one sample (for example, two swabs) for one case, only the first sample will be charged at the full price. All subsequent samples will be charged at 50% of the list price. If you specially wish multiple samples to be pooled and treated as one and therefore charged just once, please specify this on the submission form. R = Referral Test †= Non-discountable Test Telephone: 01379 854180 21
Faecal Packages at-a-glance Feline panleukopenia virus PCR Feline enteric cornoavirus PCR Giardia intestinalis (AtoF) PCR Cryptosporidium Microscopy Clostrdium perfringens type Canine distemper virus PCR Tritrichomonas foetus PCR Giardia immunotographic Clostridium perfringens Campylobacter culture Cryptosporidium PCR Salmonella spp. PCR Clostridium culture Parvovirus Antigen Salmonella Culture Yersinia culture Parvovirus PCR Parasitology antigen test enterotoxin A. PCR Laboratory Test Code Price 2020 FA01 / l l TOPFA01 FA02 / l l l TOPFA02 FA03 / l l l l l TOPFA03 FA04 / l l l l l l TOPFA04 WEC l FAEX2 l l l PCCGI l l l l l l PCCGE l l l l l l l l PCFGI l l l l l l PCFGE l l l l l l l l l GRDCR l l GIARO l CRYPO l FACA l FASA l YER l CLOSC l CLOST l When working up a case of diarrhoea the following tests may also merit consideration: B12 and folate (starved serum sample, see p13): useful in investigation of chronic small intestinal diarrhoea. TLI (starved serum sample, see p13): used to investigate for EPI. A Canine/Feline B12 & Folate (TOPB12FK9/TOPB12FFEL) or Canine TLI, B12 & Folate (TOPTLBFLK9) can be added to our Faecal Profiles FA01, FA02, FA03 or FA04 at the reduced Top-Up price providing all of the required samples are submitted together at the time of submission (it is not possible to add the test on at the Top-Up price following the original submission). Intestinal Profile 1 (0.5ml serum and faeces, see p11) Total protein, albumin, globulin, A:G ratio, faecal analysis (microscopy and worm egg screen, selective culture for Salmonella & Campylobacter). Intestinal Blood Test Profile (1ml serum, see p11) Total protein, albumin, globulin, A:G ratio, urea, creatinine, ALT, ALP, total bilirubin, amylase, sodium, potassium (+ ratio), calcium, glucose, AST, GLDH, bile acids, cholesterol, triglycerides, lipase, chloride, phosphate & CK. A Canine/Feline B12 & Folate (TOPB12FK9/TOPB12FFEL) or Canine TLI, B12 & Folate (TOPTLBFLK9) can be added to either of the Intestinal Profiles above at the reduced Top-Up price providing all of the required samples are submitted together at the time of submission (it is not possible to add the test on at the Top-Up price following the original submission). 22 email@finnpathologists.com
Faecal Analysis Sample Type Turn Around Profile Test Price Code Required Time Faecal Profile 1 Selective culture for Salmonella & Campylobacter Faeces 2 to 3 Days FA01 Top-Up Faecal Profile 1 TOPFA01 Faecal Profile 1 plus worm egg screen (does not include At least 3g of Faecal Profile 2 2 to 3 Days FA02 Giardia & Cryptosporidium) faeces Top-Up Faecal Profile 2 TOPFA02 Faecal Profile 2 plus testing for Giardia & At least 3g of Faecal Profile 3 2 to 3 Days FA03 Cryptosporidium faeces Top-Up Faecal Profile 3 TOPFA03 Faecal Profile 3 plus testing for parvovirus At least 3g of Faecal Profile 4 2 to 3 Days FA04 (comprehensive faecal analysis for puppies and kittens) faeces Top-Up Faecal Profile 4 TOPFA04 A Canine/Feline B12 & Folate (TOPB12FK9/TOPB12FFEL) or Canine TLI, B12 & Folate (TOPTLBFLK9) can be added to our Faecal Profiles FA01, FA02, FA03 or FA04 at the reduced Top-Up price providing all of the required samples are submitted together at the time of submission (it is not possible to add the test on at the Top-Up price following the original submission). Canine B12 and Folate 0.5ml Serum Same Day B12FK9 Top-Up Canine B12 and Folate TOPB12FK9 Feline B12 and Folate 0.5ml Serum Same Day B12FFEL Top-Up Feline B12 and Folate TOPB12FFEL Canine TLI, B12 and 0.5ml Serum Fasted blood sample required for TLI test Same Day TLBFLK9 Folate not Heparin Top-Up Canine TLI, B12 and Folate TOPTLBFLK9 At least 3g of Worm Egg Screen Microscopic examination for worm eggs and Coccidia Same Day WEC Faeces At least 3g of Basic Faecal Screen Worm egg screen plus Giardia and Cryptosporidium Same Day FAEX2 Faeces Giardia Immunochromatographic antigen test Faeces Same Day GIARO Top-Up Test for Giardia TOPGIAR Giardia Microscopy Faeces 2 Days GIARD Giardia intestinalis (assemblages A to F) PCR Faeces Up To 6 Days PCGIA Cryptosporidium Microscopy Faeces Same Day CRYPO Cryptosporidium spp. PCR Faeces Up To 6 Days PCCRY Giardia & Immunochromatographic antigen test/microscopy Faeces Same Day GRDCR Cryptosporidium Top-Up Test for Giardia & Cryptosporidium TOPGRDCR Selective culture for Campylobacter & Salmonella, worm At least 3g of Exotic Faecal Analysis 2 to 3 Days EXFAE egg screen, Giardia & Cryptosporidium Faeces Gram stain examination of faecal smear from exotic Faecal Gram Stain Faeces Same Day FGRAM species Faeces/Tissue/ Campylobacter Selective Culture 2 to 3 Days FACA Swab Faeces/Tissue/ Salmonella Selective Culture 2 to 3 Days FASA Swab Salmonella spp PCR Faeces Up To 6 Days PCSAL Faeces/Tissue/ Yersinia Selective Culture 2 to 3 Days YER Swab Clostridium Anaerobic culture for the isolation of Clostridium spp. Faeces 2 to 3 Days CLOSC R = Referral Test Telephone: 01379 854180 23
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