MICORPS MONITORING MACROINVERTEBRATE

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MICORPS MONITORING MACROINVERTEBRATE
MICORPS
MACROINVERTEBRATE
MONITORING
MICORPS MONITORING MACROINVERTEBRATE
WATERSHED

 Muskegon River Watershed
   Drains ~2,723 mi2 of land
   Starts at
    Houghton/Higgins Lakes
    down to its mouth at
    Muskegon Lake and,
    eventually, Lake Michigan
MICORPS MONITORING MACROINVERTEBRATE
AQUATIC MACROINVERTEBRATES

Small organisms that you can see without a microscope
that lack an internal skeletal system and live part or all of their
lives in water.
                      Why Collect Macros?
Good Indicators                                Good for Volunteers

o Good indicators of stream conditions         o Easy to sample
  (live there all the time)
                                               o Generally abundant communities
o Macro diversity = Healthy stream
                                               o It is a unique experience
o Threats to bug diversity
  o Sedimentation
  o Habitat loss
  o Chemical/organic pollution
MICORPS MONITORING MACROINVERTEBRATE
NEW DATA SHEET
 Ordered from sensitive to tolerant
  0 = Least tolerant; 10 = most tolerant
 Only 19 to know!
 Need at least 100 specimens
  < 30 – These are the worst of the worst
   samples. If you can't get 30 bugs in an hour's
   work, the stream is heavily degraded. Score a
   10.
  < 60 – Certainly a problematic stream that
   should not be given a good score. Score a 7.
  60-100 – No penalty, but always strive to get
   over 100 insects per site.
MICORPS MONITORING MACROINVERTEBRATE
MICORPS SAMPLING
PROCEDURES
MICORPS MONITORING MACROINVERTEBRATE
SAFETY GUIDELINES FOR STREAM
              WORK

Keep your collector in    Move slowly and
       sight                cautiously

  Beware of instream
  items that could be
scientific equipment or   Wear life jackets if
 dangerous (like rebar      appropriate
     and chunks of
       concrete)
MICORPS MONITORING MACROINVERTEBRATE
COLLECTION PROCEDURE

 Collector gets in the water
   Wadable water
   35-45 minutes of total effort
   300-foot stream section
   Work upstream, always heading toward
    clean water (for visibility purposes)
   Sample all representative microhabitats:

   •Riffles           • Stream Margins
   •Pools             • Leaf packs
   •Cobbles           • Undercut banks
   •Aquatic plants    • Overhanging vegetation
   •Runs              • Woody debris
MICORPS MONITORING MACROINVERTEBRATE
COLLECTION
PROCEDURES
 Shuffling, pulling, scraping, dragging,
  grabbing with hands
 The collector is not stopping to
  examine their net and never dumps
  debris back into the river...that's not
  their job!
 Collectors bring debris over to the
  team for immediate picking or put
  debris into a bucket for later picking
 Muck is miserable. Minimize muck
  mass.
MICORPS MONITORING MACROINVERTEBRATE
PICKERS DUTIES

 Place macroinvertebrates into small
  jars of 70% ethanol or 70% isopropanol
 Strive for at least 60 creatures (>100 is
  preferable)
   They don't need to count them, just
    have a good general sense
 Keep everything found within the 60-
  minute picking time limit
 Aside: a head picker or Leader (with
  experience or trained), is a very
  helpful person to have on the stream
  bank.
MICORPS MONITORING MACROINVERTEBRATE
DIRECT YOUR PICKERS TO...

 Don't prefer one type of creature over
  another
 Optional: If they pass >150
  macroinvertebrates, you can have them
  stop
 Count and release any clam, mussel, snail,
  fish, and crayfish
    Exception: Provide New Zealand
     Mudsnail pictures. They should keep
     these snails and tell you about them if
     they saw them
    If you find a New Zealand Mudsnail, get
     in touch with an EGLE biologist
DECONTAMINATION

                   New from 2021: Equipment
                    decontamination with dilute
                    bleach or other chemical like
                    409 is a required component
                    of MiCorps sampling
IDENTIFICATION

 Don't do identification during
  picking except for light education
  purposes; the goal of this time is to
  pick

 An ID expert needs to confirm or do
  all identifications

 You can do ID after collection, or
  hold a different ev ent in the
  following weeks

 You should NOT...
   Do the official ID in the field
   Do the official ID on liv e creatures
   Hav e the v olunteers only sav e a
    few insects of each type

 Aside: sav e all samples for at least 5
  years
SCORING-
What is organic pollution?          HILSENHOFF METHOD
Pollution delivered to a stream
through both point and non-point
method
• Strongly connected to oxygen                                   Scores are secondarily
                                     Measures sensitivity to
  levels                             organic pollution (0-10)
                                                                     connected to
                                                                habitat quality and flow
• From natural, agricultural, and
  urban sources
• Waste-water                       Areas with higher organic
                                        pollution will have     More bank erosion; fine
                                    degraded habitat through    sediment; flashy water
• Fertilizers and nutrients           landuse/development               flows
                                             impacts
• Pesticides
WATER QUALITY RATING
(WQR)

1.   Add Total Abundance
2.   Multiply: Count x Sensitivity for each
     line
3.   Sum the Count x Sensitivity Column
4.   Divide that Sum (Count x Sensitivity)
     by the Total Abundance
VARIATION IN PROCEDURE AND
LOGISTICS
                                                      Total collection effort should be 35-
                                                       45 minutes no matter the number
                                                                  of collectors
             Collectors must be trained ahead
               of time w/ practice sampling
                          session

                                                           300-foot stream sections

                  Follow MiCorps collection
                         procedures:

                                                       Don't pick for more than an hour
                                                         (can be less if full time isn't
                                                                   needed)
             Don't do your official ID's out in the
             field. Kill the specimens in alcohol,
               ID in a controlled setting with an
                 expert. Keep the specimens.
                                                      All macros must be collected in a
                                                      2-week window that is similar year
                                                                 after year
HABITAT ASSESSMENT

 Helps us understand WHY we are finding theses
  macroinvertebrates or why their populations are
  changing
 It is a qualitative assessment, not quantitative
 Your team will fill out a form answering questions on
     Stream Size                  • Plant community
     Flow                         • Size/condition of riparian
     Presence of foam/oil/trash     zone
                                   • Sources of degradation
     Stream bank condition
                                   • Optional pebble count

**We will walk through assessment during the field sampling
day
HABITAT
ASSESSMENT
How often?
   Once every 5 years
When to hold it?
   After collecting
    macroinvertebrates
   Summer is a good time to
    conduct a habitat
    assessment (vegetation
    types and density are part
    of the assessment)
HABITAT ASSESSMENT

How to hold the assessment
1.   The group needs to spend some time at the
     stream before answering the habitat
     questions. Explore and poke around the
     entire 300-foot reach
2.   One person walks the group through the
     data sheet
3.   The group, by consensus, rates different
     aspects of the habitat
4.   If the group can't agree on the answer, you
     take averages (when numbers) or record
     comments
MACROINVERTEBRATE
  IDENTIFICATION
KEY BODY
  PARTS
ORDER – MEGALOPTERA: TWO FAMILIES

 Sensitive   Hellgrammite-Dobsonfly/Fishfly   Alderfly                     Sensitive
0→
             • Family-Corydalidae             • Family-Sialidae
     1                                                                         1

     2                                                                         2

     3       Characteristics                  Characteristics                  3
                                                                       4.0 →
     4       • Lateral projections            • Lateral projections            4

     5
             • Three pairs of legs            • Three pairs of legs            5

     6                                                                         6
             • No distinct tail; prolegs      • Distinct single tail
     7                                                                         7

     8
             • Generally larger               • Generally smaller
                                                                               8

     9       • Often found on wood            • Often found in silt            9

     10                                                                        10
 Tolerant                                                                  Tolerant
                     Family-Sialidae
Sensitive
1.3 →   1
                 ORDER-PLECOPTERA: STONEFLIES
        2

        3
                Characteristics
        4
                • Two tails
        5       • Three pairs of legs

        6       • Two tarsal claws
                • Long antennae
        7
                • Sometimes gills are on underside of thorax
        8         but never running up and down the top of
                  the abdomen
        9
                • Often found in gravel or on cobble and
        10        wood
    Tolerant
Sensitive
        1
                ORDER-TRICHOPTERA: CADDISFLIES
        2
3.2 →   3
                Characteristics
                • Three pairs of legs
        4       • Wormlike
        5       • Often with plates on thorax
                • Prolegs on end of abdomen with claw
        6
                • Often in a case (sand, leaves, twigs, etc.)
        7       • Some types are free-living
        8       • Found in many habitats

        9

        10
    Tolerant
Sensitive   ORDER – EPHEMEROPTERA: MAYFLIES
        1

        2
                   Characteristics
3.5 →   3          • Three tails
        4          • Long antennae
                   • Three pairs of legs
        5
                   • Feathery or plate like gills on abdomen
        6            NOT thorax
                   • Found in many habitat types
        7

        8

        9

        10
    Tolerant
Sensitive   ORDER-ODONATA; SUB-ORDER:
        1        ANISOPTERA (DRAGONFLY)
        2

        3
4.0 →
        4
                 Characteristics
        5
                 • Stout body
        6        • Labium "mask" to catch prey

        7        • Two pairs of wing pads
                 • Large eyes
        8
                 • Three pairs of legs
        9        • Five stiff points on end of
                   abdomen
        10
    Tolerant
Sensitive   ORDER-ODONATA; SUB-ORDER:
        1         ZYGOPTERA (DAMSELFLY)
        2

        3

        4

        5         Characteristics
                  • Slender body
        6
                  • Two pairs of wingpads

7.7 →   7         • Three pairs of legs

        8         • Labial mask
                  • Three tails (gills)
        9

        10
    Tolerant
THREE TAIL CONFUSION...

Damselfly                           Mayfly
Does not have gills along abdomen   Has gills along abdomen
Sensitive   ORDER-COLEOPTERA:
        1       BEETLES
        2
                Characteristics
        3
                • Generally harder bodies (Elytra)
        4       • Oval shaped
5.1 →   5       • Chewing mouthparts

        6       • Well-developed eyes
                • Three pairs of legs
        7
                • Found in many habitats, but many like slower
        8       flowing water and vegetation

        9       • Both adults and larvae live in water and look
                • very different
        10
    Tolerant
BEETLE LARVAE
Characteristics

• Eye spots and antennae usually visible

• Mandibles often visible

• Three pairs of segmented legs (4-5 segments of each)

• Sometimes lateral appendages at end of abdomen
CONFUSION EXPECTED!! BEETLES,
ALDERFLIES, AND DOBSON FLIES
1. Beetle (Hydrophilidae)
2. Beetle (Dytiscidae)
3. Alderfly (Sialidae)
4. Dobson Fly (Corydalidae)
5. Beetle (Gyrinidae)
BEETLE ,ALDERFLY OR DOBSONFLY
LARVAE?
                                Alderfly

                                Beetle

                                Beetle

                                Hellgrammite-
                                Dobsonfly

             Beetle
CADDISFLY   BEETLE
LARVAE      LARVAE
ORDER-DIPTERA
"TRUE FLY"

Larvae General Characteristics
• So many kinds!!!
• Very small
• Soft bodied/worm like
• No legs
• No visible wing pads
• Many have poorly developed head
• Some have fleshy or filamentous things that may
  look like a tail
MICORPS SIMPLIFIED TAXA
LIST FOR FLIES

Listed by sensitivity
0 = least tolerant
10 = most tolerant
Sensitive   ORDER-DIPTERA: THREE FAMILIES OF
1.0→   1            TRUE FLIES (SENSITIVE)
       2
                       Water Snipe Fly (Athericidae)
       3               Characteristics
       4               • Found in fast flowing water
                       • Burrow in gravel
       5
                                                         Net-Winged Midge (Blephariceridae)
       6                                                 Characteristics
               Dixid Midge (Dixidae) Characteristics
                                                         • Suction cups on each body division
       7       • Flat terminal lobes fringed with hair
               • Prolegs with hooks on abdominal
       8         sections 1-2
       9       • Can see head
               • Found in shallow/slow water with
       10        plants
   Tolerant    • Found on surface of water when
                 picking
Sensitive           ORDER-DIPTERA: TRUE FLIES
       1                TOLERANT TO POLLUTION
       2        Mosquito (Culicidae) Characteristics
       3        • Head capsule visible
                • Fused thoracic segments (bulb shape)
       4
                • Found in slow water
       5        • Breathe air with spiracle              Soldier Fly (Stratiomyidae)
                                                         Characteristics
       6                                                 • Flattened body
       7                                                 • Leathery looking
                                                         • Can see head
       8
8.7→           Rat-tailed Maggot/Hover Fly (Syphridae)   • Found in muck
               Characteristics
       9                                                 • Pollution tolerant
               • Cannot see head
       10      • Mouth hooks
   Tolerant    • Long spiracle to breathe air
               • Pollution tolerant
DIXID MIDGE         MOSQUITO

  Sensitivity = 1    Sensitivity = 8.6
ORDER-DIPTERA: TRUE FLIES NOT LISTED AS
    Sensitive
              SENSITIVE (1) OR TOLERANT  (8.7)
                   SOMEWHAT SENSITIVE
       1

        2
                            Black Fly (Simulidae) Characteristics
        3                   •   Bowling pin shape

        4                   •   Stand up in water column
                            •   Filter fans at head
        5                   •   Found attached to vegetation, rocks, logs
6.0 →   6
                   Midges (Chironomidae) Characteristics
        7                                •   Head exposed
                            •   Thoracic and anal prolegs
        8

        9                        Cranefly (Tipulidae) Characteristics
                                 •   Found in leaf packs
        10                       •   Cannot see head
    Tolerant                     •   Abdomen end varies
                                 •   Can get large
Sensitive
        1
                    ORDER-HEMIPTERA: TRUE BUGS
        2

        3

        4       Characteristics

        5       •   Piercing/sucking mouthparts
                •   Wings hardened near the base and membranous
        6           everywhere else
                •   Three pairs of legs
7.7 →   7
                •   Adults look same as young
        8
                •   Found in many habitats, but many like slower flowing
                    water and vegetation
        9

        10
    Tolerant
Sensitive         ORDER-AMPHIPODA: SCUD
        1       (FRESHWATER SHRIMP, SIDESWIMMERS)
        2

        3
4.0 →
        4

        5

        6
                Characteristics
        7       • Two pairs of antennae
        8       • Seven pairs of legs
                • Flattened (laterally)
        9
                • Often found in silt or along stream banks
        10
    Tolerant
Sensitive
        1
                    ORDER-ISOPODA: SOWBUGS
        2

        3
                Characteristics
        4
                • Two pairs of antennae
        5
                • Seven pairs of legs
        6
                • Flattened (dorsally-ventrally)
        7
                • Leg-like gills on posterior end
8.0 →
        8
                • Adults look same as young
        9

        10
    Tolerant
Sensitive
        1
                  ORDER-DECAPODA: CRAYFISH
        2

        3        Don't collect, but count them
        4

        5
6.0 →   6

        7

        8

        9

        10
    Tolerant
Sensitive     ORDER-VARIOUS CLAMS AND
        1       FRESHWATER MUSSELS (BI-VALVES)
        2

        3

        4        Don't collect, but count them
        5       Characteristics
                • Very old, slow growth
6.9 →
        6
                • Many are special concern
        7
                • Place carefully back into sediment
        8         the way you found them
                • Don't count empty shells
        9
                • Some are tiny
        10
    Tolerant
Sensitive
        1
                         ORDER-VARIOUS SNAILS
        2

        3

        4       Characteristics
                • Many types
        5
                • Watch for New Zealand Mudsnail*

6.9 →
        6       • Don't count empty shells
        7
                   Don't collect, but count them
        8
                   Collect zebra mussels
        9
                *If you suspect a mudsnail at a location that is
        10      not known to be infested, please contact Paul
                Steen or EGLE
    Tolerant
Sensitive
         1
                        ORDER-VARIOUS: LEECHES
         2

         3        Characteristics
         4
                  • Many, many striations (surficial only)

         5
                  • Two suckers: front and back

         6

         7

         8

         9

10   → 10
      Tolerant
Sensitive
         1
                  ORDER-VARIOUS: AQUATIC WORM
         2

         3

         4

         5

         6
                  Characteristics
         7

         8
                  • Segmented

         9        • May look like an earth worm

10   → 10
      Tolerant
QUESTIONS?
NEXT TRAINING SESSION
 Marshall Memorial Park, Newaygo: 246 State
  Rd, Newaygo, MI 49337
 10am-1pm
 Meet in parking lot
 Need to bring: water, sunscreen, bug
  spray, waders (we can supply), good
  walking/hiking shoes (there will be walking)
 Official Sampling: May 20th 11am-2pm @ Ed
  Henning Park, Newaygo (Wheeler Drain and
  Brooks Creek)
 SarahKrzemien@ferris.edu
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